Microplate Reader LMR 9602G
Contents
1. MICROPLATE READER User s Manual k Model LMR 9602G Labnics Equipment Please read the manual carefully before using the instrument Some Precautions and Security Prompts LMR 9602G Microplate Reader is specially used for vitro diagnosis Please take the 10 11 following precautions before using the instrument and strictly follow them Take protective measures before turning on the instrument by wearing gloves and other defending clothes as some samples may have some adverse effects Do not randomly discard the used sample All samples negative or positive controls standards and waste should be treated as infectant The instrument wires should be connected properly to avoid electric shock especially ground wires should be connected properly with the ground The AC power supply should be stabilized Avoid using the same power supply with high power equipments especially break frequently and avoid disturbing by strong electromagnetism To drag off the power wire hold the plug itself instead of power wire lf you find smog peculiar smell or strange sound coming from the instrument please cut off the power supply immediately and contact the franchiser Cut off the power supply of instrument and cover it with dustproof cover after completing the detection process If you need to uninstall the cover for repair or other causes you should first turn off the instrument and then cut off the2. and smooth running of operation etc Display is a liquid crystal one with alohanumeric and graphic options Parameters can be checked on the display without using difficult command sequences 1 2 Main Functions 1 The instrument is mostly used for the detections of viruses germina tumour detection human body s hormones and other items based on the principles of immunology in the clinical tests 2 Corresponding detection for microanalysis in chemistry or biochemistry 1 3 Denominating Rule LMR 9602G Serial Number Designing Number Symbol of the Instrument Symbol of Computer 1 4 Working Conditions 1 1 Environmental temperature 10 30 C 2 2 Relative humidity 70 3 Keep it away from strong electromagnetic field and avoid direct irradiation by strong light 4 Avoid dust and shake There must be no caustic gas such as hydrochloric acid in air 5 After switching on the instrument wait for 20 minutes before starting the experiment so that the equipment gets warm up 1 5 Dimensions and Weight Dimension 420Lx325Hx210W mm Weight 10 kg Chapter 2 PRINCIPLE AND FEATURES 2 1 Working Principle This instrument works on the principle of Photoelectric Colorimetry It can detect the absorbance of detecting sample and standard liquid Also it can analyze and calculate the absorbance value and finally produce the values or the condition for detecting sample in the liquid 2 2 Structure Features The instrument has two ma
3. appear Then you can enter the item No 0 119 2 Click on ltem Name icon to input the name of program The name may be a combination of numbers and letters for example 8IG If you have not entered a name the system will designate a default one 3 Select working mode either as single wavelength or dual wavelength based on the reagent specification The pink box means the current selection Click the number of wavelength to change the inputted value see the functionof SET 4 Select single well detection or dual wells detection If you select the latter the instrument will combine two wells inthe row direction as A1 A2 A3 and A4 H11 H12 and use the means of two absorbance 5 Ifyou want to exit programming click on the up arrow in the status bar on the right side of the screen or click the icon at the left bottom Click on the down arrow to go to the next programming interface 5 2 2 Vibration Mode and Detection Mode Explanation 1 Instrument has vibration function But we suggest you to use external vibration if you have 2 If you just want to get the absorbance of sample and do not need any analysis and calculation please select Absorbance 3 Ifyou need to check the quality positive or negative of sample please select Ciualitative Detection Between the two qualitative modes CUT OFF Mode uses the calculated value asthe cut off value and CUT OFF Control uses the absorbance of the lower p
4. bol of 7 2 Wavelength Setting 7 2 1 Enter the wavelengths nm of filters in turn Pay attention to make the input data accord with the arrangement of the filters in the tray otherwise it will cause wrong results There are four filters in the instrument and they are arranged as 405nm 450nm 492nm and 630m 7 2 2 The instrument can maximum set 10 filters Ifthe numberof wavelength is less than 10 enter 0 7 2 3 Continue clicking Delete all items and then the numbers of 1 2 3 appears When number 3 appears the instrument will save all items information and the wavelengths set But all the detection results will be deleted 7 2 4 The function of Debug is to check the work parameters when the instrument is maintaining Click the icon of STOP to exit the function Chapter 8 INQUIRY The function is designed for inquiring the saved quality control data and detection results Click Inquire on the main menu and then the following interface appears Explanation 1 If you wantto print the quality control results click on Result of QC The screen displays the symbol of that means symbol the item number 0 5 The six items are quality control items detected in this month When you enter the item number the LCD shows the data of this item amp 2 When Detection Result is selected the screen will display the list of detection results Enter the result number you want to inquire so that it will display t
5. ct these errors before using the instrument After that if auto check process gets over machine will go to the main menu and wait for further orders At that time you can operate program detect inquire according to your demands Before you begin to detect Wait for 20 minutes since turning on the instrument so that the instrument get a complete warm up If instrument doesn t get any command for 30 minutes it will automatically comes to sleeping state in order to prolong its life When you start operating again instrument will return to its normal state Chapter5 PROGRAMMING Before beginning detection you should first decide the order of programs according to the needs for your convenience order of program numbers should be based on the following principles 1 Putthe most commonly used detection items in the front and arrange combined items together 2 Because the instrument only allows five items to process quality controls synchronously you Should arrange the items accordingly which needs quality control according to their preference 5 1 Programming Introduction 5 1 1 The following picture is the main menu of the Instrument Explanation Detect After programming is finished you Can click on the icon to start detection process It then allows to select item set the row and column number and decide whether to use the calibrating factors saved in the instrument or not Program Click the icon to enter the interface o
6. ction invalid filter must be changed by professional time to time 9 2Lamp The instrument will prompt if the light lamp fails At that time you should open the lid of the instrument and examine it If lamp is not working properly please change it with same standard lamp If something is wrong with the power repair it 9 3 Tray If there is something wrong with the tray it can t pass in and out of the instrument possible reasons for this error are may be the down lead of step motor is not properly connected synchronous strap may be loose or doffed moving parts shift and get blocked etc So find outthe reason behind this error get rid of the malfunction 9 4 Fuse If fuse burns out cut off the power supply first and then change the fuse with the same standard You may change the fuse under the professional s guidance Chapter 10 TRANSPORTATION 1 Range of Environmental Temperature 20 C 55 C 2 Relative Humidity of Environment 93 3 Range of Atmosphere Pressure 500hpa 1060hpa Chapter 11 PACKING LIST Name Number 1 LMR 9602G Microplate Reader 1 2 Printer 1 3 User s Manual 1 4 gt Product Certificate 1 5 Users Checking and Accepting Sheet 1 6 Accessories a Wire for Power Supply 1 b Fuse p5x20 2A 2 c Plastic Cover 1 Chapter 12 QUALITY GUARANTEE We will repair or replace the instrument or accessories free of cost if there is any problem with the instrument provided that you
7. e Explanation 1 The definitions of letters in above formula are as follows NCV Negative Comparison Value S Absorbance ofthe Sample N Average Absorbance of Negative control P Average Absorbance of Positive control A B F A1 B1 F1 A2 B2 F2 Parameters requires to enter according to reagent specification CO CO1 CO2 Cut off values used for judgment 2 Click on Single so that the instrument display next interface for entering the parameters A B F Now enter the three parameters according to the reagent specification Click on the down arrow in the status bar to finish the operation of entering three parameters The parameters set will not display in the process of programming but appears in detection If the calculation mode is Single means positive and means negative The Dual calculation mode requires entering two groups of parameters The first group is A1 B1 and F1 The second group is A2 B2 and F2 Take these two cut off values as upper limit and lower limit to judge quality positive or negative Inputting method is same as that of Single If the calculation mode is Dual means positive means negative blank means the absorbance value is between the two cut off values Enter two parameters at the Limit Value position Click on underline and the keyboard appears Here two parameters are the upper limit and the lower limit for subsection of absorbance respecti
8. e status bar to return to the previous interface or click on the down arrow to show information on the detected item selected When the correct information has been displayed continue to click the down arrow to begin detection 6 4 When detection starts screen display Detecting now wait for few seconds till detection gets finished Now instrument displays absorbance of every well then it will give the result of qualitative or quantitative detection Results from column 1 to column 6 first appear on LCD If you wish to see the results from column 7 ta column 12 please click on Next button at the bottom of the display The symbol of means there is na detectian at this position If you want to print the absorbance please click Print Click enter to go into the next interface or return If the detecting results need to be stored for query click save The instrument then sends all the data to the printer together You can do other operations once the printing starts and needn t wait The printing farmat shows as table 3 len Pte Wavdength Date Time Umi Opentor 01 00 03 40560610 E E l0 Oh B p f D IE F H Megative Positive Cutoff wiuve Ooabtcontrel Explanation The program decides the output of detecting results Detected results are mainly four 1 Absorbance 2 Result of Cut off Judgment and value of FP TO 3 Concentration 4 Judgment based on Normal Concentration Value H L If detected res
9. f programming The instrument can save 120 programs from 0 to 119 Set The instrument allows setting wavelengths system time and deleting all saved items detection results quality control results and the calibrating factors Inquire Query the results of quality control detection results or parameters of programs Time After clicking on the Time icon the current time will be shown on the bottom of the display This time will be appearing for few seconds and then it will return to the main menu Finish After clicking an the Finish icon the tray returns to the instrument and then does not respond for other icons till you click the icaon again to make the tray comes out and after that you can click on other functional icons Caution After clicking on the finish icon the system will save some important parameters For example results of quality control and detection results So you must click the icon of Finish and wait till the tray returns to the instrument Then turn off the instrument 5 1 2 When you need to input some numbers click the inputting position Then a keyboard will be displayed as cen Pe J You can directly click the number you want to select When all the numbers have been entered press ENTER Ifthe number needs to be changed please click the button Pet to delete the number entered and enter it again The item name may be a combination of numbers and letters Now when you click on the inputting posit
10. have complied with the instructions of this user s manual and it is within one year from the date of purchase You will be charged if you have not followed this user s manual or you have disassembled the instrument without authorization Our company will answer maintenance related questions for the instrument even after the one year warranty period Chapter 13 WARNING 1 The power wire should be earthed properly 2 Filter must be changed by the professional SERVICE REPORT Customer s Address Contact Person Tel No Fax No Weekly Off Dept Date Configuration Model Serial No STOK Q NGR AAA a Demonstration Y Maintenance Contract EA o Repairs O ro pop o Apio A Bilbe te e lt lt JAR BH AO AR Nature of Problem Observation Action Taken Customer s Remarks Parts Replaced Parts Recommended Action Required Yes A No Q Service Engineer s Name 4 Signature Requisition Number Customer s Name Signature Date Stamp Page gt Of DO O OID
11. he saved result There are 100 results saved in the instrument Click on up arrow or the down arrow to go to the other interface Click the icon of STOP to exit inquiry 3 When instrument displays the detection results or the quality control results akeyboard appears at the bottom When user wants to inquire the detection results the user should select Print or Trans and keep in mind that the function of Save is invalid But when instrument displays the quality control results the functions of Trans and Save are invalid Click the button of Next and then it will displays X and SD You should enter the two parameters based on reagent specification Then the quality control chart will generate and it can be printed 4 The format of Item Information is same as that appeared on the interface of detection Click on Item Information and display shows At this moment enter the item number 1 3 bits and click jenreq 5 Clickon up ordown arrow in the status bar to leave the interface Chapter 9 MAINTENANCE Instrument needs cleaning only in normal usage But the following assemblies will be invalid or have malfunctions after along time usage You may as well maintain them under the guidance of professional 9 1 Filter The transmission rate of filters will be decline after a period of usage When the instrument doesn t work properly it will give you a clue or prompt In order to maintain the accuracy of dete
12. in components the mainframe and the printer The mainframe composed of optic system 8 channel detection system singlechip system and enzymatic plate driving system Instrument can detect enzymatic plate that has 96 or 48 wells 2 3 System Configuration 8 Channel Enzymatic Drive circuit Photoelectric detection Steady voltage Simulative signal disposal power and A D conversion circuit Display Keyboard Rs232 serial port Fig 1 System Configuration Chapter 3 SPECIFICATIONS 3 1 Functions 1 Detect absorbance as well as used in qualitative quantitative and kinetic detection 2 Itcan store maximum 120 programmes in generaluse and display programs parameters on LCD 3 The instrument can automatically select filters for different items 4 4 On starting the instrument it will first undergo auto check process by checking initial settings and if there is some error the corresponding prompt will appear on the screen 5 The instrument has printing function 3 2 Technical Specifications 1 Range of working wavelength is from 400nm to 700nm It has 4 filters when leaving factory 405nm 450nm 492nm 630nm 2 Characters of the filters Accuracy of wave nm Half width nm 3 0 12 Table 1 Characters of the Filters 3 Detection range of instrument is from 0 1A to 3 0A The errors of absorbance accuracy are as follows Absorbance A Accuracy Error 0 1 1 0 1 0 o
13. ion of item name a keyboard will be displayed as 16 ED e Ei By 5 A 1 a 3 0 sem z us DEL In the above keyboard 1 means a conversion between number and letter When it is converted to letter the button x will appear for selecting letters from A to Z When the well s position is required to select the keyboard will be displayed as oa i a wc wo 4 E s F e v 8 9 SEE Atthis moment a letter will appear after first clicking and further corresponding number after second clicking 5 1 3 There is a status bar on the left side of the display when you are programming Click on the up arrow button to return to the previous menu and on the down arrow button to go to the nextmenu 5 1 4 You can easily set blank negative positive and quality control wells on the enzymatic plate when you are doing qualitative detection For ex you can set blank negative and positive wells from 1 to 3 respectively But the quality control well should not be more than one When you are doing quantitative detection you can set standard wells from small to large values according to rows or columns 5 1 5 In the process of programming you can click the icon on the left bottom to exit programming and return to the main menu 5 2 Programming Operation 5 2 1 Programming Click the Program icon in the main menu The next display will show forexample Explanation 1 After you click Item No the keyboard will
14. nce in the interface of Detection Mode and then click on the down arrow in the status bar to enter into the interface of blank wells setting After you set the blank wells click on the down arrow in the status bar Then save the results The operations are same as above 5 2 7 Kinetic Detection Mode 5 2 7 1 Click Kinetic Detection in the interface of Detection Made and then click on the down arrow in the status bar to enter the following interface Explanation 1 Click underline to enter tne corresponding parameters Unit of delay time and detection time is second The instrument will adjust interval between two detections based on the detection time The sampling time is 6 seconds in the detection period 2 Calculating Formula Activity of enzyme A minxF IU L In above formula F means factor Chapter 6 DETECTION This instrument can be used for clinical tests after being programmed To ensure veracity of detection results please note the following items 1 Wells of blank negative control positive control or standards on the board should be consistent with program 2 If reagent has been changed you should use the new negative control positive control and standards to calibrate 3 Strictly accord to the reagent specification to detect 4 Create indoor quality control system and assure the quality state of real detection in time biy detecting the quality control samples 6 1 Switch on the inst
15. of concentration It may be mg l mg dl etc Please enter the unit according to the reagent specification 3 Enter the number of the standard wells and positions of each well The instrument will show the positions of standard wells when it displaysthe item information before detection 4 Thereis one quality control well Yau should select whether to have quality control well or not 5 Click the icon at the left bottom to exit programming Click on the up arrow in the status bar to return to the previous interface Click an the down arrow in the status bar to go to the next interface 5 2 5 3 SetStandard Values Explanation 1 Click on Concentration to enter the standard concentrations as the reagent specification prescribed in order from small values to large values Please inputtwo concentrations each line Use keyboard to enter the values and then press Enter to finish this inputting Precaution When the detection method is logarithm method you can t input the first concentration value ifitis 0 otherwise the detection results will be wrong 5 Ifyou need to judge the normal values click Normal Value and then enter two normal yalues 6 Click Save to store the item parameters in the instrument and then return back to the main menu If you click on Exit the instrument will not save the parameters Now click on the up arrow ta return to the previous interface 5 2 6 Absorbance Detection Mode Click Absorba
16. of detection results In such circumstance enter the prescriptive value as the reagent specification demand If reagent specification has no requirement do not enterand thusinstrument doesn t perform the validity judgment when detecting The quality control well must be one When you enter negative or positive wells please enter its number first and then enter its position lf there is no change in the input items and you wish to use them when detecting please click on the icon Save Click the icon Exit to quit this programming Click on the up arrow in the status bar to return to the superior interface Thus the programming of qualitative detection mode is complete 5 2 5 Quantitative Detection Mode 5 2 5 1 Detection Mode Click Quantitative Detection in the interface of Detection Mode and enter the interface of Blank Well Click on the down arrow in the interface to enter the next interface to set calculation method Explanation 1 Click the icon at the left bottom to exit programming Click on the up arrow Jf the status bar to return to the previous interface while click on the down arrow in the status bar to go to the next interface 5 2 5 2 Set Wells Explanation 1 Click underline after Dilution Factor to enter the real dilution multiple When the detection mode is Ratio Logarithmic Technique the factor inputis used for calculating calculation coefficient revised factor 2 Unit means the unit
17. of quantitative detection select the item of Use results of this detection the screen displays the standard curve after detection For example The Y coordinate may be absorbency A or log10 A and the abscissa may be concentration C or log10 C which is decided by the selected calculation mode The line is decided by the calibrating factor The letter r denotes the lineal correlating ratio and the pink box denotes the detectian value of the standard liquid You can click the icon of Edit to change the absorbency of the standard liquids For example Click the corresponding position of the number and input a new absorbency When the new number has been entered click the up or down arrow in the status bar and return to the interface where calibrating curve appears Then calculate the correlating ratio again Ifyou click Next it will calculate the sample s concentration using the calibrating factor displayed inthis interface The calibrating factor a and b will appear and get changed at the beginning of detection Chapter7 SYSTEMSETTING Click on Set in the main menu and then the following interface appears For example 7 1 Time Setting When there is something wrong with date or time displayed on the screen you Can Select the function and enter the right date and time in turn in the next menu First click on positian and then use the keyboard at the bottom to enter The bit you enter is decided by the sym
18. ositive well as the cut off value 4 Ifyou need to check the concentration of sample please select Quantitative Detection 5 Click icon at the left bottom to exit programming click on the up arrow in the status bar to return to the first interface of programming and click on the down arrow in the status bar to go to the next interface 5 2 3 SetBlank Well Instrument allows setting blank wells randomly and thus set 8 blank wells at a time User can select the mode of Average Column Blank Wells and set all the 8 wells of ane calumin as blank wells Explanation 1 First select Randa mly Set click on the strings 564555 and enter5 the number of blank wells Then enter the positions of blank wells When you select Average Column Blank Wells directly enter the column number and no need of putting position of every well 2 Click the ican at the left bottom to exit programming Click on the up arrow in the status bar to return to the interface of Vibration mode and detection mode Click on the down arrow in the status bar to go to the next interface y 5 2 4 Qualitative Detection Mode 5 2 4 1 Calculation Mode 5 2 4 1 1 CUT OFF Mode Select the CUT OFF Mode which is qualitative detection mode in the interface of Detection Mode and enter the interface of Blank Well Now click on the down arrow in the interface to enter into the next interface to set calculation mode qualitative mode and negative comparison valu
19. power supply While working with the instrument wear rubber gloves and after completing your work wash your hand to avoid any kind of infection Be careful while handling reagents or patient samples by hand or other body parts and in case your body wound comes in contact with the patient sample wash immediately and consult doctor This is aclinical detection equipment and has no taboos Chapter 1 1 1 1 2 1 3 1 4 1 5 2 2 1 2 2 2 3 3 3 1 3 2 3 3 4 4 1 4 2 5 5 1 5 2 6 7 7 1 7 2 8 9 10 11 12 13 LABNICS Equipment Table of Content Contents INTRODUCTION Characters Main Functions Denominating Rule Working Conditions Dimensions and Weight PRINCIPLE AND FEATURES Working Principle Structure Features System Configuration SPECIFICATIONS Functions Technical Specifications Power Supply PANEL Back Panel Preparation and Examination PROGRAMMING Programming Introduction Programming Operation DETECTION SYSTEM SETTING Time Setting Wavelength Setting INQUIRY MAINTENANCE TRANSPORTATION PACKING LIST QUALITY GUARANTEE WARNING SERVICE REPORT Pg No OA RPWOWWWONMDNYND B fa TE No O U0U0U00U0Ap A OR Chapter 1 INTRODUCTION 1 1 Characters The LMR 9602G Microplate Reader is a semiautomated benchtop instrument used in clinical immunity tests in hospitals or epidemic preventing stations lt has unique features such as high sensitivity high accuracy high detection speed
20. r 0 01A gt 1 0 2 0 2 0 or 0 02A gt 2 0 3 0 5 0 or 0 05A 23 0 4 0 8 0 or 0 15A Table 2 Absorbance Accuracy 4 Linearity of the Instrument Absorbance A Error 0 1 1 0 0 8 gt 1 0 2 0 1 0 gt 2 0 3 0 3 0 gt 3 0 4 0 6 0 Table 3 Linearity of the Instrument 5 Reproducibility Error 20 5 6 Stability Error 0 005A 10min 7 Detection Modes single wavelength or dual wavelengths 8 Display 320 x 240 pixels LCD screen 9 Print External printer 10 Communication RS232 serial port 11 Output Absorbance cut off value qualitative judgment value of P CO concentration judgment of normal value and quality control value 3 3 Power Supply 1 Voltage 220V x 14 10 2 Frequency 50 Hz x 1 2 3 Power supply must be earthed perfectly Chapter 4 PANEL 4 1 Back Panel CN 07 7 Fig 3 Back Panel 4 2 Preparation and Examination First examine the instrument whether having scathes such as distortion or cracks due to inappropriate transportation and storage If there isn t any scathe insert plug into the electric socket and switch on the instrument Now instrument will start auto check process and if there is something wrong corresponding prompt will appear on the screen For example if filter fails instrument prompt as Filter Failure if the optical system has malfunction prompt will appear on screen as Optical System Failure So corre
21. rument and then first start the auto check process After auto check process gets over it will display the main menu At that time you should not detect immediately wait for 20 minutes for a complete warm up and then start detecting 6 2 Click on Detect in the main menu and the following detection interface appears For example Nunber Mane Busber The LCD shows item names and item numbers of 18 programs at a time Click on the down arrow in the status bar to see parameters of other programs Please select a program number before detection After selection click the icon to return to the main menu The instrument consist of saved values of calibrating factors of quantitative detection and the cut off values CO1 CO2 of qualitative detection If it is qualitative detection the saved cut off values CO1 Co2 will be displayed if it is quantitative detection the saved calibrating factors a and b will be displayed The absorbency of the standard will also be displayed if the quantitative detection mode is Pointto Point Technique or Interpolation Technique You can use these saved parameters to calculate click the corresponding parameter to change or select Use resultof this detection If you select the later one please add standard liquids to calibrate again and use the new results to calculate If you select the former the sample can be put on the standard wells to detect gt 6 3 Click on the up arrow in th
22. ult has concentration value as 99999 it means the concentration of the sample is beyond the linear range You should dilute it and detect again 6 5 When results need to be transferred to the external PC first connect the signal wire and then wait till the PC comes in a state of waiting Please see the user s manual of Software of LMR 9602G Microplate Reader When screen show the absorbance click then the picture of appears on the screen lt means data is transferring now Until the transmitting is finishec the picture of does not disappear 6 6 When above all work is over take the enzymatic board out If you still want to continue detection of the item replace a new enzymatic board and repeat the operating steps since step 6 4 If then also you wish to detect other items please repeat operating steps since step 6 1 6 7 After all the detection items are finished return to the main menu Click on Finish so that the tray of enzymatic board wall return to the instrument automatically if you click on Finish again the tray of the enzymatic board vyill come out Then shut off the power supply and pull out the plug 6 8 When the detection method is kinetic type the display will show the A T reaction chart after detection Click the icon at the bottom to switch the sample from 1 to 8 amp 6 9 When the calculation mode is Linear Technique Semi log Technique Total Logarithmic Technique or Ratio Logarithmic Technique
23. vely Make sure thatthe difference of two parameters comes up to 10 average shares The difference between the two absorbance value and the lower limit is put to the corresponding subsection The result is a number from 0 to 9 For example input two parameters 0 5 and 1 0 Average subsection is 0 05 If the absorbance is 0 6 the difference between the sample s absorbance and the lower limitis 0 1 and then the resultis 2 Reagent specification prescribes that when the negative control value is less than the input value for example 0 05 you should calculate according to the input value so please click on the corresponding underline and enter the regulated value But if reagent specification prescribes that the negative control value is more than the input value for example 1 20 you should calculate according to the input value so please click on the corresponding underline and enter the regulated value Click the little icon atthe left bottom to exit programming Click the up arrow in the status bar to return to the interface of blank wells setting and Click on the down arrow in the status bar to go to the next interface 5 2 4 1 2 CUT OFF Control Select the CUT OFF Control qualitative detection mode in the interface of Detection Mode and enter the interface of Blank Well Click on the down arrow in the interface to enter into the next interface Explanation 1 The definitions of letters in the above formula are as follo
24. ws S Absorbance ofthe Sample L P C Lower Positive Control Value NCV Negative Comparison Value Equivocal Range is the uncertain range that if the calculation result lie in this range it is very difficult to say about the sample quality whether it is positive or negative For example Ifyou enter 0 1 before L P C it means the equivocal range is from 0 9 L P C to 1 1 L P C That means if the L FR C lower positive control value is 1 then the calculation results from 0 9 to 1 1 are equivocal Reagent specification prescribes that when the negative control value is less than the input value for example 0 05 you should calculate according to the input value so click an the corresponding underline and enter the prescribed value If the reagent specification prescribes that the negative control value is more than the input value for example 1 20 you should calculate according ta the input value then click on the corresponding underline and enter the prescribed value Click the little icon at the left bottom to exit programming Click on the up arrow in the status bar to return to the interface of blank wells setting Click on the down arrowin the status bar to go into the next interface 5 2 4 2 Invalidity J udgmentand Wells Setting Explanation 1 Reagent specifications of some detected items have prescribed the validity judgment for the absorbance of positive contral and negative control to ensure the validity
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