LoliTherm - electronic thermometer and temperature regulator system
Contents
1. Erani 8 RELATIONSHIP BETWEEN DIV AND DCO sie edinearteueiiaaxeieenton 8 DrAR EG SPECTEICATIONS irinenn earen FEE DE 10 Page of 10 S E T U LOLIGOSYSTEMS COM Connect the WTW serial data cable to the pH 340i instrument Then use the DB9 M12 converter cable to make the connection to the pH REG instrument The round black M12 connector on the converter cable should go in frontside input on the pH REG instrument labelled IN Then connect a pH probe to the pH 340i instrument and turn this instrument on To power the pH REG instrument connect the power cord to the backside socket labelled POWER and plug it into a wall outlet or power strip Once the pH REG is turned on it will start reading the analog output from the WTW pH instrument On the backside of the pH REG instrument there are two digital controlled relays used for control of a solenoid valve to regulate pH in the water e g by injection of pure CO gas for hypercapnic control CONFIGURATION OPERATING THE FUNCTION KEYS The pH REG is a menu driven instrument The front panel has three buttons for operation e g two arrow buttons A and V and one OK button Use these three buttons to scroll through the menu and accept instrument settings For each menu there is a scrolling help text which is automatically shown in the display2. LOLIGOSYSTEMS COM PH REG ANALYZER AND REGULATOR SYSTEM user manual version 1 0 The pH REG system is used for monitoring and regulating partial pressure of carbon dioxid pCO in sea or fresh water indirectly via water pH LIST OF PARTS 1 Controller instrument 2 WTW ph340i instrument 3 Sentix pH probe fresh water 4 Converter cable 5 Solenoid valve w push in fittings 6 PU tubing 7 Air stone 8 Data cable 9 Power cord 10 User manual 11 OPTIONAL SentixH ph probe seawater CONTENTS LIST OF BARS UNIUS NENNEN NR 1 Ge IURE N 1 CONFIGURATION OPERATING THE FUNCTION KEYS 2 CALIBRATING THE PR PROBE 2 MAIN PENANCE USING THE RELA EEPE EEr 6 FAST SET POINT ADJUSTMENT FE P TED PE PT eA 7 PASSWORD PROTECTION EErEE TESE 7 DEFAULT SETTING 7 USING THE CONTROLLER FOR DATA ACQUISITION
3. gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt DECIMAL POINT POSITION DECIMAL POINT POSITION DECIMAL POINT POSITION DECIMAL POINT POSITION DISPLAY READOUT LOW DISPLAY READOUT HIGH SET RELAY IN PERCENTAGE SET RELAY IN DISPLAY UNITS SELECT PT SENSOR TYPE SELECT NI SENSOR TYPE SELECT TC SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT PT SENSOR TYPE SELECT NI SENSOR TYPE SELECT NI SENSOR TYPE SELECT NI SENSOR TYPE SELECT NI SENSOR TYPE When Pt and Ni sensor selected SELECT 2 WIRE SENSOR CONNECTION SELECT 3 WIRE SENSOR CONNECTION SELECT 4 WIRE SENSOR CONNECTION SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE SELECT TC SENSOR TYPE When temperature selected DECIMAL POINT POSITION DECIMAL POINT POSITION DISPLAY A
4. immerse the tip of the probe in the small cap that comes with the probe Page 3 of 10 ROUTING DIAGRAM If no keys are activated for 2 minutes the display returns to default state 1 0 without saving configuration changes Increase value choose next parameter gt Decrease value choose previous parameter Save the chosen parameter and go the next menu Hold Back to previous menu return to menu 1 0 without saving Power LOLIGOSYSTEMS COM Fast setpoint adjustment and relay test Increase setpoint Decrease setpoint Save and exit the menu and simultaneously change relay state HYS y AN INCR NONE Y 1 Depa es areata a Y 1 1 0 Default state 1 1 Only if password protected 1 2 Only is FastSet is enabled 1 3 FastSet and relay test disabled at password 5000 9999 1 4 No relay outputs 1 5 Not valid for these input signals Potentiometer 0 20 mA and voltage 1 6 No analogue output 1 7 Only if input signal is temperature To default state 1 0 Page 4 of 10 LOLIGOSYSTEMS COM SCROLLING HELP TEXT Display in default state xxxx hardware error SE BR gt SENSOR WIRE BREAKAGE SE SH gt SENSOR SHORT CIRCUIT gt INPUT OVERRANGE IN LO gt INPUT UNDERRANGE 9 9 9 9 gt DISPLAY OVERRANGE 1 9 9 9 gt DISPLAY U
5. air stone and to the solenoid valve output labeled A 4 Then place the air stone in the aquarium Now when the relay turns on the solenoid valve will open and air will bubble through the water to lower pCO and increase pH Instrument settings 5 Press the OK button several times until the display reads REL1 Wait for one second and the display now reads SET or toggle using arrow buttons until it reads SET and then press OK 6 Now use the A or V buttons to increase or decrease the set point value Set it to 6 00 and then press OK 7 Set the action of the relay to DECR and then press OK Page 6 of 10 LOLIGOSYSTEMS COM 8 Now enter the hysteresis value Use arrow buttons to set the value to 0 02 and then press OK 9 Press OK several times until the display reads to finish Relay 1 will be activated every time pH drops below 6 00 allowing air to pass the valve and into the water via the air stone This will cause pH of the water to increase and as it reaches 6 02 the relay will close Once pH drop below 6 00 again due to fish respiration the relay 1 will automatically open once more in this way keeping pH at 6 00 6 02 at all times FAST SET POINT ADJUSTMENT It is possible to quickly change set point values without having to scroll through the whole instrument menu With the instrument turned on press the A button once Now choose relay 1 or 2 and press the OK button Then change the s
6. high calibration value 5 Press the OK button until the display reads DI HI and the A or V button to adjust the high calibration value to 7 00 Press the OK button to accept the value Page 2 of 10 6 Then press the OK button several times until the display reads EMS COM high calibration 7 Rinse the Sentix pH probe with distilled water 8 Next place the tip of the probe in an pH 4 00 buffer solution 9 Wait for the reading to stabilize and press RUN ENTER on the WTW ph340i instrument It know shows 4 000 To save the value press RUN ENTER again 10 On the pH REG instrument press the OK button until the display reads CA LO Use the A or V button to toggle to YES and press the OK button to accept the current probe signal for the low calibration value 11 Press the OK button until the display reads DI LO and the A or V button to adjust the low calibration value to 4 00 Press the OK button to accept the value 12 Finally press and the OK button several times until the display reads to finish the zero calibration 13 Press M on the ph340i instrument to finish the calibration For more information about the ph340i calibration see page 7 in the Operating manual 14 Rinse the probe with distilled water 15 Place the probe into a sample and record sample ph when the reading is stable Both instruments will now show the same pH value MAINTENANCE Between sample measurements
7. of the same quality e buffer capacity as the water used for the experiments 2 Bubble one of two samples with atmospheric air and the second water sample with 39 o CO gas for 10 minutes to alow time for equilibration Use stirrers to mix samples 3 Measure pH values in both samples In this example we measure pH 7 000 in the sample bubbled with atmospheric air and pH 5 000 in the 3 sample 4 Plot both pH values against log pCO values in a semi log plot and draw a straight line between the two points Page 8 of 10 LOLIGOSYSTEMS COM Relastionship pH vs pCO2 5 Now this line gives the relationship between pH and pCO2 for the water in the aquarium e g if pH is measured to 5 50 it means that pCO equals 1 Example The plot below shows the relationship between pH and pCO2 measured in Denmark 2008 Aarhus 9 0 8 5 O Soft water O Hard water 9 Sea water 8 0 T 75 7 0 6 9 6 0 0 285 0 927 6 918 Pco mmHg Dept Zoophysiology University of Aarhus Denmark Page 9 of 10 LOLIGOSYSTEMS COM SPECIFICATIONS Supply voltage universal 21 6 253 VAC 50 60 Hz or 19 2 300 VDC Internal consumption 3 2 W Max consumption 3 5 W Isolation voltage test operation 2 3 kVAC 250 VAC Signal noise ratio Min 60 dB 0 100 kHz Response time programmable 0 4 60 s Calibration temperature 20 28 C Accuracy lt 0 1 of reading
8. LIGOSYSTEMS COM The pH REG instrument has two independent relays to control the activity of solenoid valves For either of the relays choose a set point and a hysteresis value Now decide if the relay Should be activated when the signal drops below a set point DECR or if it should be activated when it rises above INCR e g for hypercapnic control the relay should act on a increasing INCR signal since pH values will tend to climb as pCO values moves toward equilibrium with atmospheric values Graphic depiction of the relay function setpoint k Relay units Relay units 100 100 90 90 80 80 70 70 pem iia neers a Hysteresis 210 50 MEE ANE OMS ON TERM f _ setpoint 50 _ TRE AQ dones 10 X oes s sy AQ N 30 p On NO 20 10 oc oc 10 DCN 1 10 20 30 40 50 60 70 80 90 100 0 10 20 30 40 50 60 70 80 90 100 Relay action Increasing Relay action Decreasing Example how to avoid high pCO despite high fish respiration rates Follow the below procedure to keep pH at 6 00 in a recirculated fish tank stocked with fish producing CO at high rates Set up 1 Connect the solenoid valve to relay 1 on the back side of the pH REG instrument relay 2 is not used in this example 2 Connect a supply of compressed air to the solenoid valve input labeled P using the blue PU tubing 3 Connect a suitable length of blue PU tubing to the large
9. ND RELAY SETUP IN CELSIUS DISPLAY AND RELAY SETUP IN FAHRENHEIT gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt ENTER RELAY 1 SETUP SKIP RELAY 1 SETUP RELAY 1 DISABLED RELAY SETPOINT ACTIVATE AT INCREASING SIGNAL ACTIVATE AT DECREASING SIGNALL RELAY HYSTERESIS HOLD RELAY AT ERROR ACTIVATE RELAY AT ERROR DEACTIVATE RELAY AT ERROR UNDEFINED STATUS AT ERROR RELAY ON DELAY IN SECONDS RELAY OFF DELAY IN SECONDS ENTER RELAY 2 SETUP SKIP RELAY 2 SETUP RELAY 2 DISABLED RELAY SETPOINT ACTIVATE AT INCREASING SIGNAL ACTIVATE AT DECREASING SIGNAL RELAY HYSTERESIS HOLD RELAY AT ERROR ACTIVATE RELAY AT ERROR DEACTIVATE RELAY AT ERROR UNDEFINED STATUS AT ERROR RELAY ON DELAY IN SECONDS RELAY OFF DELAY IN SECONDS OUTPUT RANGE IN mA OUTPUT RANGE IN mA OUTPUT RANGE IN mA OUTPUT RANGE IN mA DISPLAY VALUE FOR OUTPUT LOW DISPLAY VALUE FOR OUTPUT HIGH O ERR 23 mA gt 3 5 mA gt OmA gt NONE gt RESP XXXX gt E PAS NO gt YES gt N PAS NAMUR NE43 UPSCALE AT ERROR NAMUR NE43 DOWNSCALE AT ERROR DOWNSCALE AT ERROR UNDEFINED OUTPUT AT ERROR ANALOGUE OUTPUT RESPONSE TIME IN SECONDS ENABLE PASSWORD PROTECTION ENABLE PASSWORD PROTECTION SELECT NEW PASSWORD Page 5 of 10 USING THE RELAYS LO
10. NDERRANGE HW ER gt HARDWARE ERROR EE ER gt EEPROM ERROR CHECK CONFIGURATION RA ER gt RAM MEMORY ERROR CJ ER gt CJC SENSOR ERROR Fastset Enabled F SET REL1 gt FAST SET MENU SELECT RELAY REL2 gt SETP XXXX gt RELAY SETPOINT PRESS OK TO SAVE Fastset Disabled SETP xxxx gt RELAY SETPOINT READ ONLY menus DE gt WAEHLE DEUTSCHEN HILFETEXT DK gt DK VAELG DANSK HJAELPETEKST ES gt ES SELECCIONAR TEXTO DE AYUDA EN ESPANOL FR gt FR SELECTION TEXTE D AIDE EN FRANCAIS IT IT SELEZIONARE TESTI DI AIUTO ITALIANI SE gt SE VALJ SVENSK HJALPTEXT UK gt UK SELECT ENGLISH HELPTEXT CZ gt CZ VYBER CESKOU NAPOVEDU xxxx gt SET CORRECT PASSWORD C LIN gt TEXT ENTERED BY USER IN PRESET CURR gt CURRENT INPUT VOLT gt VOLTAGE INPUT POTM gt POTENTIOMETER INPUT TEMP gt TEMPERATURE SENSOR INPUT When current selected 0 20 gt INPUT RANGE IN mA 4 20 gt INPUT RANGE IN mA When voltage selected 0 10 gt INPUT RANGE IN VOLT 2 10 gt INPUT RANGE IN VOLT 0 0 1 gt INPUT RANGE IN VOLT 0 2 1 gt INPUT RANGE IN VOLT YES gt CALIBRATE POTENTIOMETER LOW NO gt CALIBRATE POTENTIOMETER LOW YES CALIBRATE POTENTIOMETER HIGH NO gt CALIBRATE POTENTIOMETER HIGH DEC P 1111 111 1 11 11 1 111 DI LO XXXX DI HI XXXX REL U PERC DISP DEC P 1111 111 1 UNIT C F gt
11. Temperature Coefficient lt 0 01 of reading C EMC immunity influence lt 0 5 of reading Potentiometer input min 10 Q Potentiometer input max 100 Relay function Setpoint Hysteresis in 9o display counts 0 1 25 1 2999 On and Off delay 0 3600 s Sensor error detection Make Break Hold Max voltage 250 VRMS Max current 2A AC Max AC power 500 VA Max current at 24 VDC 1A IMPORTANT DO NOT connect relays to gt 500W equipment max 2 A 250 V Marine approval Det Norske Veritas Ships amp Offshore Standard for Certification No 2 4 Observed authority requirements Standard EMC 2004 108 EC Emission and immunity EN 61326 LVD 73 23 EEC EN 61010 1 UL Standard for Safety UL 508 Page 10 of 10
12. age 7 of 10 USING THE CONTROLLER FOR DATA ACQUISITION The instrument produces a 0 5 Volts analog output signal for data acquisition purposes Connect the data cable to the socket marked Out on the backside of the pH REG instrument Connector pin 1 is positive connector pin 4 is O zero The low instrument value DI LO corresponds to a O V output and the high value DI HI corresponds to a 5 V output This means that the gain becomes HI value 5 V With the default values the gain is then 1 4 pH V e g if the output voltage is 4 V the calculated pH value is 5 6 RELATIONSHIP BETWEEN pH AND pCO From the Henderson Hasselbalch equation it is known that pH log pCO K where K is a parameter including effects of temperature salinity alkalinity To use this formula it is therefore important to keep K constant If K is kept constant the relationship between pCO and pH is obtained by measuring pH in water equilibrated with different CO partial pressures e g either from a gas mixing pump Radiometer W sthoff thermal mass flow system Brooks Instruments or from pressure bottles with special gas mixtures from suppliers of lab gasses Now pCO values can be determined from a linear curve in a semi log plot log pCO vs pH and controlled automatically to set point levels Two point calibration example using atmospheric air and 3 CO gas 1 Pour sample water in two containers Make sure to use water
13. et point value using the arrow buttons Finish by pressing the OK button Test relay functions by pressing both arrow buttons simultaneously The LED diode will toggle its state When done press and hold the OK button until the display reads PASSWORD PROTECTION Using a password will stop access to some of the menu and parameters There are two levels of password protection Passwords between 0000 4999 will allow access to the fast set point adjustment and relay test Using this password stops access to all other parts of the menu Passwords between 5000 9999 stop access to all parts of the menu fast set point and relay test Current set point is still shown By using the master password 20068 all configuration menus are available If you want to enable password protection press the OK button several times until the display reads E PAS Use an arrow button to choose YES and then press OK Now set the password using the arrow buttons and press OK when done If you want to disable password protection go to the menu option E PAS again and set it to NO Finish by pressing the OK button DEFAULT SETTINGS The pH REG instrument is delivered with the following default settings INPUT TYPE POTMETER DECIMAL POINT 11 11 DISPLAY LOW VALUE 4 00 DISPLAY HIGH VALUE 7 00 RELAY 1 UNITS DISP ON DE REL1 and REL2 0 OF DE REL1 2 0 ANALOG OUTPUT 4 20 converted into a 0 5V instrument output RESP 0 4 P
14. this starts after five seconds if no key has been activated Use the menu to calibrate the oxygen probe and set relay action and level of control A will increase the numerical value or choose the next parameter will decrease the numerical value choose the previous parameter OK will accept the chosen value and end the menu To summarize the A and V buttons are used to toggle betweens options The OK button is used to accept settings and go to the next option Press and hold the OK button for 0 5 seconds to get the last option available Once the entire configuration has been entered the n display will show The following two pages show the complete menu routing diagram and scrolling help texts CALIBRATING THE pH PROBE Follow the step wise procedure below to re calibrate the pH probe We recommend to calibrate the pH probe initially before using the instrument and probe for the first time Standard two point calibration procedure 1 Connect the pH probe to the WTW pH 340i instrument and turn on the instrument Press CAL 2 Place the tip of the probe in a pH 7 000 buffer solution 3 Wait for the reading to stabilize and press RUN ENTER on the WTW ph340i instrument It know shows 7 000 To save the value press RUN ENTER again 4 On the pH REG instrument press the OK button until the display reads CA HI Use the A or V button to toggle to YES and press the OK button to accept the current probe signal for the
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