Image Frame Work - Tarosoft
Contents
1. A Portrait Left 05 Inches Landscape Right 0 5 Inches Default Orientation Bottom 05 Inches Create re ines Frene We have to choose the paper size that available in printer However IFW gem Edit Image Tools View Window also retrieve the printer default setup value at the first time we need to print Then Open Ctr o n Close n ETE select the paper orientation Portrait or Landscape Then assign margin for 4 side ave Image As Select Twain Source of paper The page setup setting value will save for next time use User can print Acquire Twain Source TET out immediately in case that still use the same page setup value Image Print Preview Image Print VEU User can print the image by choose menu gt File gt Image Print The active Report Print Ctrl P g g eee image will print out from default printer immediately User also see the outcome Load Vector SCENES before print by choose menu gt File gt Image Print Preview The preview window Exit will appear User can use Print button in the window to print also Page 48 Rev 04 2012 012. See below topic for detail Send all result Excel 1404828 00 1404328 00 2074 681 388 146 18718300 130208 00 79 73 6 32 9 87 18386 31 01 1404 327 O00 0 00 000 0 00 ooo 0 00 0 0 000 0 00 Default Operator v New Column Remove from Last Operator Edit Range ApplyAll Only Selected Automatic Measurement Measured Area Total Objects Objects In Range Area Sum Density Mean Diameter Mean Perimeter Mean Per rea Sum Manual Measurement Area Average Length Average Diameter Average Density Average Angle Average Delete last column button Add new column button Select default operator We can assign the operator for each item by select the operator by press Edit button which appear on top between image s measure result The operator edit window will appear You may see the option for edit on top of window The option allow you to apply the selection to the rest of items or the selection will happen only item you do Apply All your edit for only this item will force all items use the same operator Only Selected your edit for this item will effect only this item Page 33 Image Frame Wrok User Manual Pass Fail Test The Pass Fail Test feature allow user to apply IFW to work for industrial job This feature come with pre define measurement value Once the measurement do by user Pass Fail Test will add the
3. Use Tolerance Radius Accept Range o0 0 o0 o UseMiniMax Use Tolerance Page 35 Image Frame Wrok User Manual Atthe bottom part of dialog You will see the I O Configuration section The I O option allow the IFW to trigger external devices such as warning light horn etc The trigger also separate between OK or NG of overall measurement Please note that this option can t work without I O hardware Please contact Tarosoft for more information O Configuration o Enabled 0 Devices for Outside Light and Sound Signal Device Info n a This feature need optional PO device please contact your local resaler For more information Output for OK E m jm jm Output for NG E E E m Manual Measurement Mode Now you can use pass fail test to measure like a routine job In manual mode user can use most common measuring tools to work with pass fail test feature All tools will act like it be in normal measuring mode The result of the test can get by two ways User can DDE the result table to Excel Or print out as hard copy or pdf by report generator UECE q USO T Ea DO Note Vernier and Manual Tage not supports Pass Fail Test feature However it s still working properly while work under Pass Fail Test environment General Config Automation Config Page 36 Automation Measurement Mode Image Frame Wrok User Manual Consider about the
4. 3 pixels Font Name Font Size Arial fis Bold Italic The Scale Bar Options need to press OK the save the new setting value If you close this window by close box The previous value will be used Allassiged value will not use immediately after complete setting User need to select any calibration value within this window or choose the objective lens button and press the Show Hide Scale bar at the same time The reason is scale bar need to know the calibration value to draw the bar on image However once the calibration value choosen User can show hide the scale bar at anytime by press scale bar icon button Objective Lens Selection button Show Hide Scale Bar B eX Page 24 Image Frame Wrok User Manual Once scale bar disply on image User can use mouse to move the scale bar to any position of image The last position of scale bar will automatically saved The scale bar create on new image will use last position by default BAR o aa 91a 9s T9 00 roo fo 909 A A E 1 Scale Bar can move to any position by mouse Only on the bar 5000 um The scale bar is display on image as drawing object The scale bar will not save with image However if you need to put the permanent scale bar on saved image You need to burn scale bar by use Burn Scale Bar to Image command before save This option will warn you before process with following info On
5. 2 Assign ROI for work with automatic count feature The Automatic Count will work only in ROI area This will make the software work faster by area smaller Then all measurement result will compare to ROI area not whole image area So you can have more accuracy outcome by use ROI for measurement The value can direct input in these textbox if you know the exactly value F Img 008 jpg 25 aid L3 9 uuu Ee00 suu GLCP GLUON GLO ooo 00 7 Re POI estion pine Lett 92 width 472 Top 360 Height 476 Current Available Color L p a V Automatic Update All ROI This option will re assign the location and size of ROI on each opened image to be the same ROI also can use mouse to resize and re locate Color of the frame can assign by ROI Properties window This ROI type also design to work with the routine job by save the last size and position This will save more time to work with same ROI area for whole series of images So if you have a lot of images to work on You will no logner worry about the analyze are anymore Page 27 Image Frame Wrok User Manual ROI Mask Once you need to work with free shape area to analyze ROI Mask is one ofthe useful tool ROI Mark start with a very simple drawing tool Our drawing will base on separate layer which be on top of image It s will not modify any part of our image ROI Editor ROI Drawing Tools olos VA m Tool to draw ROI A
6. Compromise Fluor Mon Linear Strong Fluor Mon Linear Compromise User can select one from 5 User can balance the weight of mixed methods This option transparent level by move the Apply your setting can change anytime before slider This is very easy to use New Window after Apply apply So that user can have a but powerful tool perfect mixed image by trial one of them F Mixed Image Untitled2 50 DER FRPP LP e EPERRA E EA EER ie PEER A AOR F RR h RA he PA e The mixed image still have a lot of detail from both original images 3 0 0 t 0 0 8 0 0 Page 19 Image Frame Wrok User Manual Convert Images to AVI Movie In case that you have a series of images taken by time lapse capture function You can encode all images into one movie by AVI Maker function It s have a very simple 3 step to make movie Please see the below image illustrated Toots view Window Help mposite Choose menu gt Tools AVI Maker Image Mixer 1 Select folder contain images AVI Maker Select Source Folder contain series of time lapse images Create AVI Now 2 P re S S c re a te A VI No WwW dd C Documents and SettingsNTaroNDesktopNNew Folder Stop P meses to encode all images into Images in Folder 15 Files Found Untitled 11 jpg one movie file ntitled12 jpg Untitled13 jpa ntitled14 jpg Untitled 15 ipa Untitled 16 ipa Untitled17 pg Untitled18 jpg Untitled
7. Image Enhance In case that the image not proper to analyze we can enhance with many functions in IFW Please see below for all commands Histogram Display brightness level of each channel in chart form Please be informed that their has no any indentity to tell us for good or bad image Normally the everage one could display as mountain form However we could only interesting and concentrate on the tone which we need to deal with This tool design for get the information from image No any modification to original image Gray Scale Display image Tools View Window Help RGB Chart Display Acquire Image L R or G or B Chart Display Live Video Full Screen Preview Adjust n bs Background Style Image His ogram Brightness Contrast One of useful tool for image enhancement is Brightness Contrast Adjustment Brightness adjust the intensity value for all channels While Contrast push bright level to more brighter push dark to darker Gamma work like Contrast but this function will try to maintain medium tone as much as it can Dark Cut Off will try to hide the low level of brightness which have a major meaning like noise This function very useful when we work with fluorescence Image T Brightness is the brightness adjust which use the final result from others command then make it brighter This function also work well with fluorescence image Once we get the suitable Brightness Contrast value You can check the check
8. Pass V Display Big Sign gt Master Range Setting general value setup for this feature as show on Area Accept Range uc T 0 T UseMiniMlas Use Tolerance E A E Length Accept Range 25 500 UseMiniMax Use Tolerance the right hand side This window contain with Diameter Accept Range UseMiniMax Use Tolerance Density Accept Range UseMiniMax Use Tolerance tti Th ed t ti s t t t k Angle Accept Range UseMiniMax Use Tolerance many se Ing interes Ing poin o take a Radius Accept Range 0 0 UseMiniMas Use Tolerance IO Configuration Enabled VO Devices for Outside Light and Sound Signal look is Restriction The restriction will Device Info nia This feature need optional I O device please contact your local resaler For more information explain more on next page omo sm m i m Dutput for NG can E Page 34 Image Frame Wrok User Manual Restriction Restriction SomeFailz Fail Some Pass Pass w Display Big Sign Restriction is the most important setup for pass fail test feature Their have 2 choices of definition Some Fail Fail means all measuring features must be in tolerance range If even one measurement value goes beyond min or max or percentage The overall test result will Fail Som Pass Pass means at least one measurement result be in tolerance range The overall test result will judge as Pass
9. The Display Big Sign option will show the big Pass or Fail sign on the righ top of the program window This can help the user can check the result easier e Restriction Rule N e Last Measure The most important at the 2nd point which user have to be careful At the right top of window You will see the option box to let the user set for Restriction Rule or Last Measure The Restriction Rule will work on Restriction setup which you can set it at general config dialog Select this option means the overall result will judge by restriction setup value If user select Last Measure means the overall result will judge by use the last measurement result only Not all measurement suitable for any option So user must select which option suitable to their work In the middle part of dialog You will see the Master Range Setting These text box allow user to fill the pre define standard value for each type of measurement Also user can set the way to make a calculation for each measurement Master Range Setting Standard Value Minimum Maximum Tolerance Area Accept Range 15000 o o 20 UseMin Max Use Tolerance Length Accept Range 0 250 500 0 UseMin lvlas Use Tolerance Diameter Accept Range 0 0 0 o UseMin llas Use Tolerance Density Accept Range 0 0 o0 80 UseMiniMax Use Tolerance Angle Accept Range o 0 0 o UseMiniMax
10. we can click on button In case that the measure accept range change We can click on this button again to re measure Their has 2 method for Automatic Count 1 Summary Count by use this selection we can have the statistic result from auto count with 3 major measurement Area Density and PerArea The summary count have a very fast speed compair with normal automatic count So this is a best choice if the resule cover the demand Page 31 Image Frame Wrok User Manual E n3 x Segmentation x Automatic Count img 005 jpg 50 a alor aa a a Color Picker PPE Eye Droper Size Mask Color 78 Current Available Color res HU T ih ee urent Available Color ua Cl nm Manage Count wal Bl x 5 Manage Mask Bix al Eke So e ar go am mm AH UseH UseHS v Use HSY H minimax 17 359 070 S minimas 48 100 V minimax 46 163 Select Color Range Bi Summary Count Faster Object Measure Count 681 Objects Counted 146 Objects In Range Measure Accept Range Min Max IV Area sar 0 1000000 Densiy 0o m5 Diameter 0 1000000 Perimeter 0 1000000 Per rea 1 1000000 jw Use for Next Save Needed A Range Cw Q e C ccw M Auto Apply Mask On above sample image the yellow mask area are segmentation mask area The viol
11. IFW We have many way to obtain images in the IFW 1 Open exist image file which currently store in our computer Click on File gt Open as shown below The open file dialog will display to let you choose the image file to analys in IFW Following standard file extension can be use in IFW e g BMP JPG TIF PNG GIF AVI f Image Frame Work a671 Edit Image Tools View Wwindo Open h Ctri O 5 Close T to open images as same as menu can do 5 U We can double click on workspace background 2 Image Aquisition by Digital Camera The Tarosoft Image Frame Work software support all model of The Imaging Source Digital Camera You just only install the driver come with IFW The camera will ready for you to capture the image from microscope or any image produce instruments IFW also support WDM compliant Please contact Tarosoft for more information Wecan selectthe way to get the image into IFW The alternative choice can be still image time lapse capture or movie capture The time lapse interval can be re config You also made predefine the location for captured images While movie capture can select any codec which available on your computer IFW also support full screen live image mode which suitable for any presentation or classroom The capture functions also available for this mode Image Acquisition Button Twain Source Full Screen Preview iy mw Page 9 Image Frame Wrok User Manual
12. box name Remember Last Eg Tools View Window Help Acquire Image Q ROI Rol Ei El a Live Video Full Screen Preview Histogram Brightness Contrast z Rotate Flip gt Equalize a we Bloakceok 4 gt 9 Brightness value between 100 to 100 Contrast value between O to 10 bita zl 4 nE Contrast value between O to 10 Gamma EN Dark Cut Off value between O to 128 T Brightness value between O to 255 Remember Last Adjustment Page 10 Image Frame Wrok User Manual Adjustment This option will keep the last setting for work with next image Normally many users have a similar image for analyze This is the best idea to adjustthe image which need to enhance before measure So your research outcome will more stable with no bias adjustment for all images EE xk T Brightness 4 gt o OK D vacuo gt _ Camel Y Brightness 5 Save the last use value for next image Qe sf d sms Q Gamma af of isi IV Remember Last Adjustment DSLC12022448 jpg 50 IF Untitled 50 DER BE m m E m m analysis basis We will adjust or enhance image for easiest to measure or analyze by software This understnading will save more time and man hour work Above images the image enhancement meant to eliminate the background items Then the objects human axon ready to measure by any tools Equalize This tool will re calculate all ton
13. cide odi deed Puteo Une dusund bes N 40 Configurable Properties T 00182 tertiae etu pude uasa e ua ud uda 4 Manual Ero PP 44 Density Measure TOG cuoio titio itia e addi uasepx Ua dada 45 Density Ratio Tool 254m vis utar tan Dae ea Dc Dieta tiu aan duet aula dd Astana 45 Aditotaton L60ls saer na ie eeu pa bet usus uoa Ret A E SOUS KE S 46 Interest PUNCHOM ode dubai ed beh riv Dd A ved datur 47 Image PH onte iod Vadis tt hale uasa xo tle shat halons Fuse Rasa RE secu RI id 48 Image Frame Wrok User Manual Software Installation Insert IFW CD to CD ROM Drive in Explorer you will found setup exe in root directory of CD ROM Doulbe click on setup exe to start installation process In case that your computer has a right control You can right click on setup exe then choose Run as administrator t Tarosoft R Image Frame Work Capture Edition Setup Welcome to the Tarosoft R Image Frame Work Capture Edition installation program Setup cannot install system files or update shared files if they are in use Before proceeding we recommend that you close any applications you may be running Exit Setup Directory C Program Files Image Frame Work Change Directory Exit Setup Click on installation icon Computer icon to start installation This step you also can choose the installation location by click on Change Directory Then choose the desire location If after you choose the in
14. direction of this option Youcan guess which color the segmentation will use by see the drawing circle on color wheel The Auto Apply Mask option will let us save more time In case that you have a routine research This option will choose objects on image base on previous setting automatically s img 005 jpg 50 Sa Segmentation j Color Picker FPR i aj Kk Eye Droper Size Pa Mask Color Current Available Color 4 a Manage Mask Bix e a Fite C3 50 7 39 Am oo BH UseH S UseHS V Use HSY H minimax 17 359 07 0 S minimas 48 100 V minimas 46 163 SS The yellow color on above sample image is the segmentation mask In case that the rest of images have same measurement method You no need to save the segmentation setting This jw Auto Apply Mask option also save the last setting for work with next image automatically Page 30 Image Frame Wrok User Manual Automatic Count After segmentation mask created We can use automatic count feature by call the Automatic Count vwaw ToqeAiondiclonvwirehwecandickon gg button Please see explanation below Automatic Count x Select Color Range E Button ot call Segmentation window from Automatic Count window Mask Color In Range Object color mask Out of range will not paint by this color Current Available Color Imi From left ot right first b
15. measurement summary window will show you for overall statistic for what you done with your images duck verage 7 Area Average 17 400 00 pixel sqr Length Average 352 96 pixel Diameter Average 137 00 pixel Once you choose any measurement tools The measurement properties window will appear This window allow you to choose the measurement tools s color label color cross hair etc More information for Features Color which Features Color Default Available Color separate into 2 color value Default and Current You may need to select the color of 4 Current indi Label Font ms Sans Serif v Bold Italic 8 Label Color Current Available Color a I Cross Hair Cross Hair Color Current Available Color amp T all measurement tools Then use it for all measurement Y ou need to un select any thing by use select tool then click on image Do not select any item on the active image Then the arrow of features color will point to Default This time any color selection will use for the rest of measurement In case that you select any item on image Then choose any color The changing will happen only item you choose then Label Font allow you to change font attibute of all measurement The changing will affect to all measurement s label Cross Hair is useful tool for align you mouse pointer to the far position on image to make sure that y
16. microscope magnification adjustment If we not select the right calibration value for measurement The result of all measurements will incorrect However if you still work with current measurement resul Y ou can change the calibration value to correct the result as well Spatial Calibration Name Screen z The measurement result must meet the world 4 Screen DS Ri1 0 7 0 5x standard unit To select the correct pre IDS Ri 0 7 1x calibration name before measure is needed If DS Ril n 7 2x you work with wrong calibration value You may E loose your time to re measure again Scale Bar and Objective Lens Tools Bar Some of images may need to put scale bar imprint on the image This very good idea do by draw some line to represent the calibration value for each image Image Frame Work provide the convenience way to work with scale bar and calibration value You can map the calibration value with the Objective Lens Tools Bar Then you can just click on tools bar for select the calibration value Let see how we can do that Thank to Scale Bar and Objective Lens button to let the user z3 ij u nj og can select the calibration value easier ibid you can display the scale bar with one click Moreover it also easy to look what calibration you currently use now Page 22 Image Frame Wrok User Manual Each particular section explaination 1 This is main switch for use or not use the scale bar and objective lens
17. nBiguuit IF Circuit01 bmp 100 Cer ENTERREGRERRLIREREURRREUBRRGUNRREUNRRENRREUHNIRI C17 5 R1154 USB R C15 c16 C22 NLA EERE as DE H eu C20 G thes Qm a T14R3 m h olf pak di F x RTC OLED DISPLAY r jg R2 ON EE o Oe 6164307 BEN TREES a BUTTON 6 BUTTON C BUTTON 3 0 0 3 5 E Projects Application Image ld Screen pixel Page 4 Image Frame Wrok User Manual Walkthrough the Image Frame Work IFW has a easy and easy interface which design for every level skill level of user Most of all command use mouse and toolbar to measure and analyse the images All toolbar icon has a very good intuitive design to let the user easy to understand by only see the icon Circuit01 bmp Image Frame Work elk File Edit Image Tools View Window Help GE c m 468 ocom OQ nw PUER RE BOE Raw WTZw sEaMNE eS5 o ooosozngJSsgO Please see below for each button function PUR br File Open command which accept BMP JPG TIF PNG GIF AVI mi Save As button which accept BMP JPG TIF PNG GIF ld Image Aquisition Twain source acquisition Report Print Report Print Preview Ea Full screen live display which suitable for classroom or presentation Copy Object Page 5 Pw m MASK m A s dd Image Frame Wrok User Manual Paste Object to selected image Navigation Window which lo
18. take longer time Range for first But good enough to use forever The first time you just finish your draw lines The overall result may say Fail x Draw Style Free Style Force Horizontal Force Vertical This means the standard value still exceed the tolerance Tolerance 20 x IV Bicubic Average Hide Boundary Box I EZ Ese Hae Asea euh Dis es Cons T Anvisa Faba T zum 1 E The easiest way to work with pass fail test You must start to work with the image that you trust May taken from the best one After finish draw edge detect lines You can test the measurement by press Test Mode on configuration window The measure result will fill up for standard value Also fill the min max tolerance value atthe same time Now your master sets of data are ready to use for QC job Automation Pass Fail Test Configuration Il Design Mode b Test Mode TAY Get Value Remove Al X Remov Name Standard min Max Tolerance Determine Type 310 00000 300 00000 320 00000 0 00000 Use Min Max 280 00000 270 00000 290 00000 0 00000 Use Min Max 126 00000 120 00000 130 00000 0 00000 Use Min Max 270 00000 268 00000 272 00000 0 00000 Use Min Max 60 00000 58 00000 62 00000 0 00000 Use Min Max 4 Save Automation Objects As Load Automation Objects File LiveCapture Confirm Measuring Before Start Delay 10 Second before First Capture m Optio
19. that you mis typo for unit e z New 2 s Measure Spacial Pixels Unit these textbox may not effect to real calibration value e Delete Lenght E Measure which we will do by next step The next step is very important step We need to understand the process cleary before do it Let take a look for next step Page 21 Image Frame Wrok User Manual DS Ri1 60x 1280x1024 bmp 25 Seg Calibration By Image Le E A A feo ooo eo m After Finish Your Mark Process Please Fill inpet the Box with How Many Unit the Mark Long 50 Bom prinese Also we will see the straight line on active 4 m image We have to move the end point of After press Measure button We will see the calibration by image window appear straight line to fit with the known distance on image In the sample image we drag the straight line to equal 5 vertical lines in image This mean the line represent 50 microns En BW RR RR RR REIR 3 length compare to real world distance Then input the 50 in the textbox Then press OK to end the calibration process 4 s In case that you have more than one objective lens or zoom able stereo microscope You may need to calibration for each objective lens or each zoom ratio The most important issue to be careful Every time you change the objective lens on your microscope or you adjust the zoom ratio on stereo zooming body Y ou must change the calibration value to match the
20. 3 ipa Untitled4 jpg Untitled6 jpg LIntitled ipg ntitled8 ipg Untitled3 jpg Images List 3 You must select Codec before encode all images into movie You may need to choose Full Frame Video Compression Uncompressed to maintain quality of each frame in movie Compressor Full Frames Uncompressed That is very important if you meant to use it for analyze in the future If you want to make the movie for other purpose You may use others codec which available on your machine Please refer to movie player software for play the movie by difference computer Spatial Calibration Unit Calibration Image Frame Work come with the spatial calibration capability So we can calibrate all tools in software to understand world standard units e g micron milimeter centimeter inch etc The process start with user capture the image by use current hardware configuration NV 4 The calibration value from other difference configuration may not use with our current measurement That s can cause a mistake in all measurements Page 20 Image Frame Wrok User Manual We may need to use Stage Micrometer when we work with microscope for calibration process Since microscope give us with a very high magnification range The others scale for calibration may not suitable for Only stage micrometer we can use The stage micrometer look like below picture Stage Micrometer in the picture is one
21. DE Now button Please note that the decimal digit adjustment use the round number function Once you decrease the digit The last decimal digit may not equal the real measurement The button before DDE Now button is DDE options setting The DDE option allow user to Decima 5 a DDE Now Sheet Mame Sheen X Start Row 1 Start Cot 1 Reset The example of Horizontal Vernier measurement The adjust line present as 2 vertical lines User can use mouse to adjust the lines to equal with the object You may see the number on the right hand side of the measurement User also use the mouse to drag the middle part of vernier That will move both lines for adjust the position while maintain the distance between lines select the sheet name row column reset You may start to send the measure data via DDE by reset the value first Then every time you measure and use DDE The row value will auto increase one row Tr img 002 jpg 50 LPR LP Ee Pt Y P ne nee pes pee Arn n pee pon oec AA i All measurements result always use the calibration value for calculation Please refer to calibration section for the detail measurement data will transfer to Excel with no lost This will prevent the data over written Y our complete set of SEE Page 43 Manual Tag Manual Tag is the tool to mark the objects by user IFW provide two table type for this function User can click on gn butto
22. Image Frame Work User Manual Zarosoft Contents OE yy are Ts tal ALTO joe rece lestie ted M veo into ee deo od ensi diae 3 Analysis Start NON ec csutte sich date iulse inus n aa dai eaa tiui eese curd 4 Walkthrough the Image Frame Work sssmMMM 5 Obtain Imase to IFW 425 senden dicente td a N terials Ged ueniat ers 9 Image AMC es uibs Ete ene eed oae bd Chaat publ Potala Nita 10 Convert to Gray Scale Image see tice et reiqeu eet deiueu ieget oim tue 15 Convert Image to Binary with Threshold see 16 Exide Colom C Wants lias ciudad A career efe sienna A 16 Merge Color Channel with Color Composite ccccccccccccccccceeeeeeeeeeeeeeeees 17 Merge Images by Image Mixer ciiisde ic ceicnssadcalvaiicttalonitalcGidatndedalsadbelceisatbdedeloastenetel 19 Convert Images to AVI Movie nennen nnne nennen 20 Spatial Calibration Unit Calibration 2 0 0 0 ccccccssccesssccessseceseeeeseeeesseeees 20 Scale Bar and Objective Lens Tools Bar eeeeeeeeeeeeeeeeess 22 Analysis ASSISE FOOLS 5s idein norit oss pov Uni utu eue sorde S EE datu ue 26 ROF R cion or Interes oca ossstibis topuestvsa te t dur tcsa ca RO CU o dat uod Du S 27 SE DING MALTON ERR 29 PALOMA IC COIL serios bs uc ptas eeu opa bet usns A N 31 Measure C algu OE socis dotted dde cay edi dutedutiv suu ues dtum ut Dod utum edo 33 Pass B atl DOS ous quts ioa gta A Uber Ne E AUOD EEUU 34 Measurement TOOLS isses sus
23. QC routine job in production line Some work may need 100 inspection That s will consume a lot of time if you can imagine to The Automation Mode of pass fail test need the proper digital camera camera stand light source and sample jig The concept of automation mode is let the operator put the product on jig then press Automatic Measure button All pre define edge detect lines will start to measure all points you want Then in case that you need a hard copy of report Printer will print out the report automatically Print preview also available as an option After finish this process Operation can start to measure the next item immediately This is the fast and accuracy routine which instand result report print out good bmp Image Frame Work File Edt Image emensus LI SRAM OCHBHBENENHTIRASYNEYYXEXXYYTYSEIsEBEB Eo v IFEEEL 50 m E il KOA ECA EA A EA COT GG CO LG 9 ns 0 s Lag Name Are fugi Length Diameter M Density Angle degr Radius Min 13 er a ee T 93 e mis aS P ut 03 2 re E ou 3j n Ww E J s o E mscr Paar 04 j 1 i E I Desi p TexsMode we Xe gt E Name dard Min Max Tolerance Determine Type a EAL 0 0 00000 0 00000 0 00000 Use Min Max 23 EeA2 0 00000 0 00000 0 00000 0 00000 Use Min Max i o oF 23 1 had d E 7j 9 4 d Save Automation Objects Save Automation Objec
24. axis scale type It s come with one hundred vertical lines Each line has a 10 microns spatial distance You may see the on glass slide printed 0 01 mm which means 10 microns Please be noted that in biological research One axis scale good enought for measurement In the industrial may need at least 2 axises for calibration Once we observe through microscope eyepiece We may see the vertical lines as shown in left hand side image By FOV field of view of high magnification lens We may not see all lines its have So the calibration process will do only the scale we can capture by current FOV only co View Window Help Color Composite a Q ROI RO E B ux i TH AVI Maker VA For calibration process we choose ROI On off menu gt Tools gt Calibration Options Or Remote Control press Calibration Button on tools bar red Calibration Options Ontions arrow The calibration window will appear The new calibration process start with New button Then follow this step 1 Fill the Spatial Calibration calibration name in Name textbox 2 select physical f Measure Spacial Pixels Unit Lenght 1 unit 3 push Measure button to go to active captured image for next calibration process The Name and Unit meant to tell everyone to Spatial Calibration recognize this calibration and physical unit you need to Name EEA calibration to However in case
25. beyond range will become black color We call this image type is Binary Image So that we can measure the objects in image very easy then before Adjust minimum value by mouse Whole range adjustable by mouse image Tools View Window Help A Adjust maximum value by mouse Acquire Image Live Video Full Screen Preview Threshold Histogram Adjust gt Rotate Flip gt Tone Histogram Chart Filter gt Convert to Gray Scale Segmentation Click on Apply button Extract Color Channel gt Min 24 Max 187 to start to generate binary image Direct value input by keyboard also can do M DSLC12022448 jpg 50 E X F Threshold Untitled12 50 DER 900 a IFW will keep the last value for work with next image automatically This will useful if you have a lot of images to work with similar condition In case that you may lost the last number by work with other analyze condition You can put the direct value input for min and max You will get the same condition back as you wish Extract Color Channel We can work with only each channel from color image by use Extract Color Channel function The purpose meant to let us see on each channel that which channel can work better for analyze Sometime the interested objects may stand in only one channel while other channel obstruct our analyze We can increase the measurement performance by work with right channel Page 16 Image Frame Wrok User Ma
26. button This option is un check by default You may need to calibrate your microscope before turn on this option Once this option turn on You will see the scal bar and objective lens button on tools bar 2 Choose which objective lens magnification number will show on tools bar However this selection must match to your real magnification you have The wrong selection may cause other user use the wrong calibration value 3 These combo boxes allow you to map the calibration value with objective lens magnification which show on tools bar Please make sure to map the calibration value to the right objective lens icon to prevent any wrong measurement result On the right hand side ofthis section You will see the textbox to input the scale bar lenght This number will use when you need to display scale bar in image The scale bar length appear on the image will correspondance to objective lens you select on tools bar Image Frame Work use the real micron sign to display on the scale bar once user calibrate the unit with micron So the correct micron sign will appear instead of U This mean your scale bar will look more professional than others software 4 Default option is useful function for the routine research job Once you choose the default objective lens All images will be assigned by this objective lens automatically However this option may cause the wrong measurement result after you change the magnification at your microscope w
27. cate where we working on the zoomed image Magnification Window which display higher magnification of mouse location Assign Region of Interest to restrict the area you want to measure Assign Mask Region of Interest to restrict the measure area by free form shape Color Segmentation the tool to choose color in the image for auto measurement Automatic Object Count Measure Calculator the tool for store the statistic measurement of each measured images Then we can use this tool to do mathmatic between each result of images Spatial Calibration tool Object Select tool Zoom tool Point to Point Measurement tool Page 6 Su Beer fh A f o N Rectangle Area Measurement tool Ellipse Area Measurement tool Polyline Measurement tool Polygon Measurement tool Angle Measurement tool Auto Edge Detection tool Outter Auto Edge Detection tool Inner Horizontal Vernier Vertical Vernier Manual Tag tool Manual Object Count Density Measurement tool Density Ratio Measurement tool Image Frame Wrok User Manual Page 7 amp ool Lim HHH Text Annotation tool Arrow Annotation tool Line Annotation tool Box Annotation tool Ellipse Annotation tool Multi Line Annotation tool Polygon Annotation tool Overlay Scale Display tool Microcsope Objective Magnification Selection tool Image Frame Wrok User Manual Page 8 Image Frame Wrok User Manual Obtain Image to
28. ce Scale bar burned to image This scale bar option will no longer use E Tools View Window Help The burned scale bar will permanent merge into image So Acquire Image user can t disable the image scale bar ever Live Video Full Screen Preview Histogram So the Burn Scale Bar to Image command need to be Adjust Rotate Flip gt consider before do it Because any image with no scale bar can Filter gt open and show scale bar at anytime if you know its calibration Convert to Gray Scale 2 i Segmentation value Once after use this command the active image will has the Threshold Extract Color Channel gt permanent scale bar on However it need to save the image to Merge Vector Layer to Image complete this process Page 25 Image Frame Wrok User Manual Analysis Assist Tools T im Jj ml Image Navigator Bu AONO OC IC UN COL NC x The tool for display whole image and red rectangle to inform us about the real zoom image displaying on active window Sometimes you may need to work with high zoom ratio Then it hard to find other interesting positon The Image Navigator will help you to locate next interesting position by drag mouse on red rectangle ofnavigator window instead rf i S C Ej 7 The red rectangle show in navigator window 7 mean the current area of active image window This red frame will change it s size correspondance to active window zoom a
29. clear edge This filter can help un sharp image has more stable edge for measure or analyze Moreover the measurement of all images in series also stable edge measurement value T Circuit01 bmp 100 E X F Untitled 100 DER ERRAR REA KAA KARA A E R NERAK ARA E ERRA A OP A ER AA a EA A AR E A a AEN On cze CN a C14R3 i ay i i pt Image Modify by Mean Filter T Gaussian modify the image to become more blur like Mean Gaussian provide the other method compare to Mean but can modify the image more blur User can try that which one give more better result for modify edge of objects in image However the blur level of Gaussian design to modify image less than Mean can do Circuit01 bmp 100 B X F Untitled3 100 DER EA E A A A RA ARA AA ARARA EA E ER EA RA KEA G A E CRISP GI Ce a a czak 87 S ELE s c2e 6 Q Bs 1 C14R3 SEL K Image Modify by Gaussian filter o Coe LET i T 17 fh T Circuit01 bmp 100 E JE X TE Untitled4 100 DAR EEA Gc EEA AEA AA E ERA RRA ERRA KRA KARRA AREA E EA AEA AEA E ERA E EA RR E A EA RA z c17 t23 USB x TM T 024 tt La d 18 E c20 6 deo t 2 H C14R3 TH Rast Image Modify by Unsharp Mask filter Dtg OLED DISPLAY A BUTTON B BUTTON C Storie BUTTON Page 13 Image Frame Wrok User Manual Emboss modify the image to look like 3D image with highlite shadow This filter will re
30. d Type3 BB Typed Type4 Typed Type5 f mW OK Cancel However user can make a choice between row move or column move at Options dialog Thank to Object Percentage which automatically calculate the percentage between tag value in row and column This will help user more easier to use the result without re work agian The DDE also provide the percentage value to Excel Page 44 Image Frame Wrok User Manual Density Measure Tool One of Fluorescence Microscopy technique we use nowadays is qualtitative measurement Researcher will use fluorochrome to tag interesting objects Then use specific wavelength to push the energy to those fluorochrome Then the enery released from fluorochrome by light form see more information about Fluorescence Microsocpy at www microscopyu com IFW provide the Density Measure tool to measure the amount of light on image T Fluor0001 jpg 50 DAR EEE A a A AR PRI PRI REPRE H PER PRI A L PRI User can click Hon toolbar The density dialog will appear Then user can draw the rectangle probe on any interesting point on active image The result of density measure come with image thumbnail and its statistic By the way the density result also display in normal measure result window So all of density measures are a part of RSS RES BIB cod cot od REIN measurement which can process like other tools This tool also provide the partial Sample 8 600 histogram Use
31. e in images Then average from bright to dark tone for make the image more clear In case that the image already has average tone You will see not thing change r P1 1post10e JPG 25 OX untitieds 25 BAR RE EA ORL petens Page 11 Image Frame Wrok User Manual Invert This is will reverse all color in image to opposite brightness value It will change from bright to dark or from dark to bright The result will look like x ray image The purpose of this function for sometime we can t see something clear while it be at the normal state Then we invert the color in image we see more clear detail of interesting objects T DSLC12021918 jpg 50 0 X TF Untitled 50 DER A A EN ILL PE A i a I Noo LI DN IM 1500 E EY J9 rj Filter In the image analysis software filter mean the calculation method to change the image in term of enhancement or in term of convolution This means some filters adjust the only visual of image Some filters change the physical of objects in image The purpose for process the image to ready to analyze at most possible dad Tools View Window Help Acquire Image JA RoI RO E I Live Video Full Screen Preview Histogram Adjust Rotate Flip gt Gaussian Convert to Gray Scale Page 12 Image Frame Wrok User Manual Following are each filters explanation Mean modify the image to have more blur Some digital image has a low resolution or un
32. ess of the object The higher number of this value will tell the detect machanism accept more wider range of color However too much tolerance also reduce the accuracy to the detection Bicubic Average may increase the accuracy of detection by try to averaging the color value surrounding the current position This option meant to reduce the noise problem which always come with digital camera Noise can make the detection miss undersanding all the time Hide Boundary Box will hide the end point boundary box Normally the boundary box will appear every time the measurement complete drawing The purpose for boundary boxes for adjust the size of any vector objects on image However sometime the boundary box cover the important position which you may need to judge that the detection is correct or not The Specific Color option provide more accuracy for edge detection This tool need to get train by use before use User need to click Choose Color to display the color training dialog The way to use this dialog is very similar to Segmentation Please refer to segmentation section for detail Page 42 Refer to Configurable Properties Tools page the rest of the tools are Horizontal Vernier and Vertical Image Frame Wrok User Manual Vernier Once user select these tools The below dialog window will appear Vernier Result Decimal 5 m Ei DDE Now You may see the decimal digit adjustment and D
33. et boundary objects 2 Auto Apply Count are the object which count by automatic count You may see some objects have no boundary This means those objects are not in range of auto count All measurement result will have the pixel unit as a default If we already done the calibration process and choose it All the result will Automatic Count Summary base on the lastest calibration unit Only degree and percentage will Total Objects 681 not effect with the calibration value Objects in Range 146 Measured on Area 1 404 928 00 pixel sqr Automatic Count Result 146 Objects In Range Area Sum 130 209 00 pixel sqr rea Minimum 10 00 pixel sqr ce Mi el Mesh Deni Mat Diane erie Statistic ZAA Ai Area Maximum 38 431 00 pixel sqr Density Minimum 42 s 3 Density Maximum 134 4 46 Density Mean 73 23 28 122 55 Diameter Minimum 3 18 pixel f 3 50 Diameter Maximum 1 419 03 pixel 10 50 Diameter Mean 3 87 pixel T 477 6 68 Perimeter Minimum 10 00 pixel 431 1 419 03 Perimeter Maximum 4 458 00 pixel i 477 Perimeter Mean 31 01 pixel l 414 Sum 9 26802 s Minimum 0 00071 923 z Maximum 2 73544 E37 z Mean 0 01361 E 2 Object Measure Count by use this selection auto count will measure object by object for individual following result Area Density Diameter Perimeter PerArea and Circularity The summary window also appear for i
34. fig befor use Please see below detail RGB Color Space once select this option The detect machanism will work with Red Green Blue color model to find the edge along the straight line we draw This option good for the high contrast object compare with background Hue Color Space once select this option The detect machnism will work with Hue Saturation color model to fine the edge along the straight line we draw This option good for the low contrast or very similar color objects compare to the background The Tolerance value need to adjust the percentage before work with both RGB Color Space HS Color Space option This value will affect to the accuracy of edge detect machanism While Bicubic Average reduce the affect from noise which general found from digital camera Page 41 Image Frame Wrok User Manual Color Selector 1 Open Image From File White Background Image Silhouette Image Original Image r FU x T VY 3 5 L 2 Color Pickup Bn gt Ep n 4 JV Autometic Build 4 Save Value i maas p W 1280 4 X H 1 024 ut UseH UseHS Use HS The RGB Color Space and Hue Color Space are no need to choose any color Once user draw the straight line across the object The detect machanism will learn the surrounding color Then shrink the line from both side until found the object Again both detect method must adjust the Tolerance to fit the edge sharpn
35. for future use by click on Save button Also we can load the segmentation setting value from file by Open button on this window The segmentation setting file has seg extension So you can copy the file for work with others computer 6 Filter Segmentation mask may contain a lot of small particle We can use Erode Dilate Open Close to clean up the segmentation mask The kernel size for filter also select able The kernel size give us like a strong level of filter Page 29 Image Frame Wrok User Manual Image Frame Work use HSV color model for segmentation The HSV contain with Hue Saturation and Value The advantage of HSV give us more convenience way to select color Please see below for its detail 1 Use H this option will bese on color selection only The function will focus on color while blind how saturation they have or how brightness they have 2 UseHS this option will focus on color and saturation level of object 3 UseHSV this option will focus the selection on all range of Hue Saturate Brightness Value of object Then use this range to select objects on whole image The Hue color space look like color wheel It has 0 360 degree of color wheel angle adjustable Base on this theory Our selection can choose between CW clockwise or CCW counter clockwise That will make the segmentation color selection create the difference object color chooser by change the
36. ison User must know the exactly position where your specimen control be However please see the R symbol on the right ame Lir hand side of each density ratio item User can click on the symbol to ee make it become the reference Then the ratio value will re calculation areas Ratio 0 63 Name Dr4 Mean 83 Ratio 0 61 again You may see the DDE button at the bottom part of window This button will send whole density ratio data to Excel Name Dr5 Mean 56 Ratio 0 41 Name Dr Mean 63 Ratio 0 46 Annotation Tools IFW also provide annotation tool to let use can add text arrow eo M amp X OF 0 graphics on image Polygon These tools are very easy to Polyline use In case that you familiar with Ellipse Rectangle measuring tools These tools no d longer hard to work with Text Page 46 Image Frame Wrok User Manual Interesting Function IFW also provide few interesting fucntions to let the user have more process for measurement Merge Vector Layer This function use for merge any measuring objects or annotation into image May some user need to get the image with measured objects paste into image for use as evident This function will create new window with merged graphic onto So user will have non merge and merged for any usage purpose To use this function click on manu gt Image gt Merge Vector Layer to Image Please noted that this function will active o
37. ithout cancel this option To cancel this option user can press button Clear Default Lens The default lens value will no longer use Use Scale Bar amp Objective Lens Options Clear Default Lens Objective Lens Options Show Use Value of Scale Bar Length Default Ds Rit 0 7 0 5 Ji 5 um aa wg ja jum inr wen i 3m sig mB Iv E E Iv L iv E Iv L E Iv ee MEE E EE M M 1 8 s C2 C8 8 Cs E C CE Page 23 Image Frame Wrok User Manual After you already assigned the value for all objective lens The value will save into Windows Registry automatically when you close the software Please be infomed that all calibration value you done on current computer not suitable to work with others microscope configuration 1 You can assign the scale bar with or without background The default is No Background This option depend on your image main tone If you need to separate your scale bar to display clearly The With Background also good choice to work on 2 This part allow you to assign the color of scale bar Also the background color in case of With Background option choosen The Line Thickness may need to adjust to more thicker in case that the image have a bigger size 3 You can set the properties of font to display the length of scale under scale bar Scale Bar Options MoBackground 100um With Background Fore Color El Back Color D Line Thickness
38. lter will eliminate small particle or dust from image while maintain object size for measurement 5 CircuitO1 bmp 100 X F untitieas e 100 eek mmm SP ll Pt eel a Image Modify by Dilate filter Re Ri i cece Dun Convert to Gray Scale Image For quantitative measurement color in image not much have meaning Most of researcher choose to work with monochrome camera to measure density on interesting area Human eyes hard to measure the difference density level if each object has a difference color So that we can measure density by gray scale image We can use Convert to Gray Scale function for this job image Tools View Window Help Acquire Image Live Video Full Screen Preview IL E Convert to Gray Scale working on only color Histogram A iin R image This function will disable when the gray Rotate Flip gt scale image activated Filter gt Convert to Gray Scale Segmentation Me gt Fluor0003 jpg 25 DER Gray Untitied10 e 25 ox EE o LP M TE M d RIBERA IPIE PRU E 8 0 0 IE Page 15 Image Frame Wrok User Manual Convert Image to Binary with Threshold Once we need to work with image which has a lot of particle or un wanted background One ofthe useful function we can choose is Threshold This function allow use to select the minimum and maximum value of gray scale level in image Then convert only value in range to become white color The
39. n to start the manual tag The result table will show like below sample Measure Result Measure Manual Tag Total Count 34 LO ADM TEST Image Frame Wrok User Manual F gt Circuit04 jpg 25 DER w00 peoo T Delete Last Clear All pua 1 The result window display the tagged result as table You may see 3 buttons Delete Last Clear All DDE at the top part of window We can choose the group or type on result window to start tag first Then click mouse on the active image to tag The manual tag allow user can delete tagged item from last tag only Then delete one by one to avoid any mistake for tagged items We can double click at row header to call the option for manual tag or click on menu gt Tools gt Options The same dialog will appear User can config the table as single type tag or multi type tag Multi Type tag design for advance manual tag which allow user can tag up to 7 groups and 5 sub types in one time User can rename any groups or sub types here While mouse pointer be on active image User can right click to select the next type on result table Density Type C Single Type Tag Move Right Object Percent ANZ Multi Type Tag Group Right Click Direction gt Group B Move Down Group C Group D Group E C None Row Group F C Column Group G 4 Type Name MW Type Typel Type2 Type2 MH Type
40. nform user for statistic of measurement In some complicated shape object you may face a long time measurement process The auto count will show the suggest dialog to let user make a decision to use Summary Count instead Page 32 Image Frame Wrok User Manual Measure Calculator Once you competed for measurement for some amount of images The idea to sent the measurements data to Excel by DDE function are quite common for general case However sometimes you may need to calculate the measurement result Then use the final result instead IFW also provide you with Measure Calculator to let you calculate each result from images immediately You can click on H button to call Measure Calculator as show the window picture below You may start to use Measure Calculator by set first column to have some measurement value from any image Then Add Column for new empty column You can select combo box on top of column to see what you wantto fill in the column s row The calculation result will ready for use now Select the statistic measurement s of any measured images Constant also available Measurement Calculator Automatic Measurement Measured Area Total Objects Objects In Range Area Sum Density Mean Diameter Mean Perimeter Mean PerArea Sum Manual Measurement Area Average Length Average Diameter Average Density Average Angle Average Button to edit the operator
41. nly at least one drawing object created on active image image Tools View Window Help Acquire Image Live Video Full Screen Preview Histogram Adjust gt Rotate Flip gt Filter gt Convert to Gray Scale Segmentation Threshold Extract Color Channel gt Merge Vector Layer to Image k Save Vector Load Vector User can save all measuring objects into files including annotations Also user can load for redarw on any image Please note that this command not specific the source file You have to make sure to not load the measuring objects into image which taken by difference magnification in case of microscope Otherwise the measuring result may wrong Then cause the mistake to your result Fie Edit Image Tools View Windc Open Ctr o Close Save Image As Select Twain Source Acquire Twain Source Page Setup Print Preview Print Ctrl P Save Vector k Load Vector Page 47 Image Frame Wrok User Manual Image Frame Work provide the image print out User can select the paper size by choose manu gt File gt Page Setup The page setup dialog will appear Then choose the appropreate paper size Gm Edit Image Tools View Wind Page Setup Open Ctrl O gt Taper Size G Close lad Width O Inches Margins Height O Inches Top 5 Inches Save Image As Select Twain Source Acquire Twain Source Print Preview
42. ns JV PrintReport V PrintPreview V Display Big Sign Once you have a good sets of master data Don t forget to save it for future use To save to current computer press Save Automation Objects You may need to pass this data to other computers by use Save Automation Objects As then choose the location to save While you may have the saved data from other computer You can load it into this computer by Load Automation Objects File i Save Automation Objects As Load Automation Objects File Options Page 38 Image Frame Wrok User Manual m Options LiveCapturee Confirm Measuring Before Start PrintReport V PrintPreview V Display Big Sign Delay 10 Second before First Capture In automation pass fail test configuration window Y ou may found the options for adjust the work style Please see below explaination Live Capture every time the operator press the Automatic Measure It s will start with capture new image from digital camera Then measure the new image with pre define features In case that you work with saved image This option no need to check Confirm Measuring Before Start just display the confirmation dialog box for sure Delay xx Second before First Capture some digital camera take some time at the initial state This setting will prevent the dark image capture at the first time you work Print Report generate the report then print on default printer Print P
43. nual image Tools View Window Help Acquire Image Q ROI Rol esl Hm Live Video Full Screen Preview Histogram To extract color channel from color Adjust image Choose menu Image gt Rotate Flip Extract Color Channel Then select Filter b the channel you need to extract or Convert to Gray Scale choose All Channels for all Segmentation channel Threshold Extract Color Channel gt Ro ae Red Channel Green Channel Blue Channel F gt Fluor0007 jpg 25 CES E Red Untitiedt3 e 25 fox IBI ORI SAC free feat am Oo A RS S RIO You can see the example for all channel extract from microscopy fluorescence technique We can see that each channel of color image contain each dye from each fluorescence probe Merge Color Channel with Color Composite Color Composite is the process which work opposite way compare to Extract Color Channel Nowsday many microscopy fluorescence technique need higher sensitive digital camera The result need to obtain maximum signal from specimen Then keep the result by use monochrome digital camera The monochrome digital camera give much more higher sensitivity compare to color digital camera when compare with same level of specifications To prove many experiment researcher need to merge each channel to observe the locallization of each channel Image Frame Work also can do this job with following step Tools View Window Help Choose Tools Colo
44. our measurement will fit to the position you want Page 40 Image Frame Wrok User Manual Configurable Properties Tools IFW has some tools to let the user config the tool s properties before use Once user can config the tool to the suitable situation These tools will very convenience Also save your time as you can feel All measurements result always E use the calibration value for r calculation Please refer to calibration section for the detail Vertical Vernier Horizontal Vernier Length Auto Edge Detect from Center Length Auto Edge Detect from End Point The first and second tools from above image sample are Length Auto Edge Detect Options X Detect Method RGB Color Space tools The tool will ready to work with similar kind of images you may have Let Hue Color Space Edge Detection measurement tool If you learn more for detect method of these see what the difference for both tools Length Auto Edge Detect from End Point this tool allow user to draw the straight line across the object Then the tool will use end point to detect the edge of object This mean the tool start to find the edge from both side ofthe line until found object Choose Color Legnth Auto Edge Detect from Center this tool allow user to draw the 7 i E straight line across the object Then the tool start to find the edge of object from Hide Boundary Box center of the line The detect method need to con
45. place all normal area to become gray color while change the edge to be colorful with shadow T Circuit01 bmp 100 EL ES OA c20 En Q C14R3 ao D Image Modify by Emboss filter Edge Detection modify the image by focus on only edge ofeach object by colorized the edge This filter give us with a edge contour So we can have easiest way to work with pure edge in image T Circuit01 bmp 100 EL ESTE DER FARRE RRRA ARRA KAAK A A a POPP k HARA ARAA Gizemi i 53 USB Ror cis NE 7 mes 9 ou t20 8 4 o D o C14R3 Image Modify by Edge Detect filter Erode will modify the image by increase the black portion of objects in the image while decrease white portion of objects in image at the same time This filter good to remove small particle or dust float in the image We can use this function with Dilate then we call Open to remove small particle in the image while maintain objects in the image to be the same size Circuit01 bmp 100 Ex TE Untitled7 100 DER ERE i RN H PIER h PERR i eR i R E ARN ii AA ir TA m NL ra as Oz C20 G E Di o C14R3 j eg R2tf i Ld Image Modify by Erode filter j IEEE p44 BOOST FO Page 14 Image Frame Wrok User Manual Dilate will modify image by increase white portion of objects in image while decrease black portion of objects in image at the same time If we use dilate with Erode we call Close This combination of fi
46. r Composite Page 17 Image Frame Wrok User Manual 1 Open all images which need to mix 2 4 images 2 Choose Tools gt Color Composite d x 1 Filename Red Untitled 3 When Color Composite window appear Please follow the number on above sample image No 1 for select the window name No 2 for select the color channel No 3 for add color channel to buffer image Then No 4 for apply the buffer image to real mixed channel image Mixed Channel fromColor Composite function Page 18 Image Frame Wrok User Manual Merge Images by Image Mixer Researcher can correlation the images which get from difference microscopy technique e g image taken from Phase Contrast and image taken from Fluorescence technique Then to approve some experment for sure Research need to overlay these two images IFW provide the tool to do this job call Image Mixer This function allow use to selecttwo opened images file to do the superimpose by vareity method of overlay Also user can balance the overlay image transparent of each image by slider The perfect mixed image can produce in a second c3 View Window Help Color Composite F Choose menu gt Tools gt Image Mixer AVI Maker Image from Obligue technique Preview Image Image from Fluorescence technique Image Mixer 1st Source Image 2nd Source Image Method Default Alpha Blend Auto Stretch Fluor Strong Fluor
47. r can click on chart button eae i on density dialog Density Histogram adi L nail af ileusne window will appear m iid m in ueggo2R QU Density Histogram r aju Chart Table 255 Density Ratio Tool The Density Ratio tool is an idea to solve the sample preparation issue Althoug you are expert in sample preparation process You may not trust that your work ofthis time and next time have a 100 same condition preparation Page 45 Image Frame Wrok User Manual The best idea to measure density researcher will put the control within the sample The control is same chemical thing as they use with specimen It s will pass all process temperature time rinse as same as specimen do Then they use control as a reference for measure density on same specimen We can call this idea as Density Ration measure IFW also provide the tool to work for specimen with control User can click on button to call Density Ratio window The new density ratio window look like an empty window User can drag mouse on interesting point on active image to create density ratio items The rectagle will present one of density ratio item User can create the density ration item as much as needed User can see the measuring result close to thumbnail image Then we have mean density and ratio value compare to top first density ratio item The first rectangle you draw on active image will be the reference Density Ratio of compar
48. rea Magnification Window This tool will work opposite function compare to Image Navigator by give us with more higher zoom ratio This tool will help you to locate your object boundary in the image more accuracy Y ou also can adjust the zoom magnification ratio by slider which locate underneath the Magnification window F img 005 jpg 50 Bx Maanification win PP E R R A E Mouse Guide Window This tool design for new user who need to not waste their time and memory Once you work with new software of course you need to learn many things Thank to Mouse Guide window for help you more easier to use this software It s will tell you tool by tool how to use the mouse to work with them So you no need to remember how to use it anymore You will familiar to use this software by no force Mouse Guide Select Tool Left Click or Left Em Drag for Select Item Right Drag for Move Image Page 26 Image Frame Wrok User Manual ROI Region of Interest ROL is the way to work with image by smaller area than whole image IFW has two type of ROI to work with ROT and ROI Mask They have own way to use as following ROI We can assign the rectacgle ROI for 2 difference function as below 1 After ROI assigned we can use Edit gt Duplicate command to make a copy of image only ROI area Normally duplicate command is the command to copy whole image to new window ROI give you an alternative choice
49. rea ROI Erase Tools ajo s Pi E Tool to remove ROI Area Display Mode nin mnu Mask display method Manage Mask Ei x Use or Hide ROI Mask Drumcue NU o yi Assign brush size This brush use for draw and erase tools T Transparent Level 53 Assign the transparent level for Display Mode 3rd button Draw Width 1 Assign line thickness of brush Mask Color Current Available Color oe Choose RO Mask color la ROI Editor x l7 img 005 jpg 50 DER EGLI ITO j 300 00 900 608 POF S00 SL 300 SL 1000 TOO a nmoljse mu ROI Erase Tools ajol e z Display Mode ninm uu Manage Mask Bii x Brush Size 100 Transparent Level 53 Mask Color Current Available Color e aaa Once we use ROI Mask to work with Segmentation or Automatic Count tools By above image sample Those tools will work only in green drawing area Page 28 Image Frame Wrok User Manual Segmentation If we want to work with Automatic Count tool You may need to learn more about Segmentation tool first The segmentation tool is the tool to choose object in image by color To assign objects color please read below step 1 Color Picker click on Eye Dropper button tool then click on any part of object we want to count The object will filled with color which we selected at the ma
50. review show the sample report before print Display Big Sign display big sign of Pass or Fail on top right corner of main window This will help operator easy to see the overall result I good bmp 50 DER T PREPRE i PEPER ES PIRE PIER PERI PP i OPPEN o PERR i OER PILLE OO e RIAL FREE Pass Fail Test Result Automatic Measure s Length Diameter M Density Angle degr i i Max Tolrance Resuk 309 00000 i 309 00000 heata 278 00000 278 00000 268 49580 58 53990 amp DDE Page 39 Image Frame Wrok User Manual Measurement Tools Image Frame Work provide the measurement tools for length area angle radius etc These tools are simple but accuracy Also easy to use at the same time These tools ready to use without any configuration d mx EE TE TE bu TE TE TE dE we ovv Angle Measure Polygon Measure Area Polyline Measure Length Ellipse Measure Area Rectangle Measure Area Vertical Length Measure v3 All measurements result always 9 use the calibration value for HONO LENN Measure calculation Please refer to Length Measure calibration section for the detail All tools which you see on above sample image are easy to use tools You just select one tool of them Then draw the tool on active image The result will appear via measure result window The measure result come with additional summary window The n Measurement Summary
51. sk color section We have to continue the color pickup until segmentation mask cover all part of our objects The gray rectangle be on right hand side of window will work only once you move the eye dropper on the active image Normally this rectangle will show only gray box You may adjust Eye Dropper Size to fit the area of object In case that you need to start new segmentation The better idea is clear the previous value by press the Eraser button The button locate next to eye dropper button Youalso can use Undo button to return back to previous status of selection if any mistake selection happen 2 The segmentation mask color can change by select on Mask Color section If you want more color You may click on rainbow button call More Color 3 Manage Mask you can manage the segmentation mask by this section To create segmentation mask click on EE button To hide segmentation mask click on 7 button Segmentation Color Picker EE Eye Droper Size Pu Mask Color Curent Available Color mne Manage Mask Bi x a Fite C3C 5C 7 f 9 aH UseH S UseHS Y Use HSV H minimas 17 3593 07 0 S minimas 48 100 V minimas 46 63 Range cw Q C ccv Auto Apply Mask 4 Wecan create new copy of segmentation mask by click on ES button The pure segmentation image will create as new window 5 We can save segmentation setting value
52. stallation location Please click on installation icon again Then press Continue for pass to next installation step g Tarosoft R Image Frame Work Capture Edition Setup will add items to the group shown in the Program Group box You can enter a new group name or select one from the Existing Groups list Program Group arosoft Existing Groups Camfrog Video Chat 5 1 FLY Player Google Chrome Internet Download Manager Microsoft AppLocale Microsoft Office Microsoft Web Publishing Object Desktop Startu C eee D DE Page 3 Image Frame Wrok User Manual You may see below window dialog appear to inform about installation completed Tarosoft R Image Frame Work Capture Edition Setup x Tarosoft R Image Frame Work Capture Edition Setup was completed successfully These are all processes for SIA installation The software ready to use now Analysis Start Now To start the IFW click on Start menu Windows XP Then choose All Programs gt Tarosoft gt Image Frame Work Fi WinsCP Xilisoft A Acrobat Distiller 8 A Adobe Acrobat 8 Professional 5 a 2 All Programs Adobe Audition 1 5 f Intel Performance Library Suite gt f Image Frame Work k Ff Linksys gt IFW program window will appear ft CircuitO1 bmp Image Frame Work BEE File Edit Image Tools View Window Help cam Sm cmomusEREB E hQwueusWwwwwuuwevrogmijanm ocoo u
53. ts As Load Automation Objects File j Y Options 3 sh War LiveCapture Confirm Measuring Before Start Delay 10 Secondbefore First Capture 04 Ji al V PrintReport V PrintPreview V Display Big Sign E T C 31 d on og 53 3 The pre define process start from Automation Config button 1 The Automation Pass Fail Test Configuration window will appear Then use the edge detection tools 2 to draw the lines across the objects on active image 3 The lines information will add to the table of configuration window 4 Object Attribute The Object Attribute will appear every time you draw the lines on active Name fen SSS image This dialog box allow you to set the name standard value all tolerance EN Standard 0 00000 value After finish input the number click OK button to save the value Y ou Minimum 0 00000 SCENE 0 00000 H can double click on the list to edit as any time Tolerance 0 00000 x Determine Method Cancel Page 37 Image Frame Wrok User Manual Please note that the edge detect measurement which assign under pass fail test Edge Detect Options X Detect Method feature It s will save individual setting for each item Because each position in C Hue Color S a image may need to get the proper setting for edge detect separately So please spend vins at Specific Color your time to fine tune the measurement as you wish Of course it s
54. utton for do automatic count If Auto Apply Count y option be selected User no need to click this button The auto count will start a after this window appear Next button is the hide auto count button Manage Count E Automatic Count come with 2 method count options The Summary Count ig La E x z R give us a statistic for object count while Object Measure Count give us with object by object measurement plus statistic like summary count However Summary Count Faster 1 summary count work much faster than object measure count many many time Object Measure Count In case that the object measure count work for too long time It s will suggest 681 Objects Counted user to change to summary count instead 146 Objects In Range Display all objects in image and In Range objects count Measure Accept Range I neris Min Max Measure acception range for differentiate in range objects with rea sqr iu bus following criteria Area Density Diameter Perimeter PerArea and Density o 255 Diameter 0 1000000 Perimeter 0 1000000 PerArea 1 1000000 Use for Next Save Needed Circularity V Auto Apply Count Option to force the auto count to use this criteria range for next image 1st Step 0 07813 Sec 2ndStep 134375 Sec f ME All Process 142188 Sec Automatic start auto count once this window appear at the first time Rects Count 6 199 To start automatic count
55. value to its table and calculate the accept range by pre define value Then give us the final result for Pass or Fail Pass Fail Test Result Name Area sg Lengh DiameterM Density Ange degr Radius 303 00000 30300000 309 00000 278 00000 27800000 278 00000 129 00000 129 00000 129 00000 268 49580 26849580 268 49580 59 53990 5353990 59 53990 V Automation Mode General Config Automation Contig User can click on Fi button The pass fail test window will appear They provide an alternative choice for manual test or autoation test mode That has a difference way to work with this feature User can select type of measuring by check or un check the Automation Mode which locate on the bottom left ofthe window lt General Contig General Config v Automation Mode Manual Test Mode Automatic Test Mode Once you need to work with manual test mode AIMost of all measurement tools can work with this feature However the automation mode allow user to use only edge detection tools at this moment However its good enough to test many many conditions Automation Mode is the best way to save time and man power to measure the objects The pre define edge detect will measure all lines in one click Back to normal learning step User can Pass Fail Test General Configuration r Restriction cli ck on G en eral C onfi g button t o display the Restriction SomeFail Fail Some Pass
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