Spot Analyzer TiVi80 User Manual
Contents
1. TING Spot anabrer T1 OX aii ra m a Spot Analyzer TiVi80 User Manual User Manual 3 2 Version 3 2 October 2013 PIONEERS IN TISSUE MABILITY IMAGING TIVi80 Spot Analyzer Dear Valued Customer Welcome to the WheelsBridge Spot Analyzer TiViSO system designed for automatic analysis of spatial and temporal variations in spot appearance The Spot Analyzer TiViSO was designed especially for observer independent analysis of changes spot erythema in association with evaluation of skin care products and follow up of treatment regimes The Spot Analyzer TiVi80 utilizes a highly sensitive digital camera equipped with polarization filters making it possible to suppress direct surface reflections from the skin surface The versatile system software based on the MATLAB high performance language for technical computing allows for rapid and easy capturing and analysis of images Among the many useful features of the Spot Analyzer TiViSO software the following are particularly helpful in the evaluation of spatial or temporal variations in spot erythema Automatic capturing of photos Zoom in features to display details of image by clicking on the actual spot Automatic batch calculation of TiVi values from photos Automatic generation of spot intensity and extension Project window displays the overall results of a test panel trial All data can be exported to ASCII format spread sheets We are convinced that the2. 27 HEE Se man Hra An pally ene ice rear sale Set RGI Spe iy AGEE Cai S Dipy TiVi image Parameters Muin Cover Span e20 kma ba E Ir dat bg uy am aera LE PBB ITA Pe OE eA or Vie Angie Cekcalant amp Cipy Tvl mage Paramena i Top Wira ina corape 400 Som G Bled Views Surai Erase Size NEUE 13 Top View click this radio button to bring the system back to top view display mode Spot Analyzer TiVi80 3 2 Sida 21 TIVi80 Spot Analyzer 14 If high TiVi value areas not belonging to the reaction of interest appear in the TiV1 image these can be eliminated by placing the mouse pointer at the point in the TiVr image to be erased Press the left mouse button and move the mouse The erased areas are displayed in gray colour and in the further analysis treated as background by the system i e not accounted for in the intensity and area calculations Position the mouse pointer on the red spot in Photo 4 press the left mouse button and move the mouse to start erasing a selected area in the T1Vi image The Spot Analyzer TiViSO main window should now look like Fie Settings Manual About Dero Aasittant Cas Feu Prec 1 15 To remove the erase section click the Reset TiVi radio button under the actual TiVi image 16 Erase Size use this pull down menu setting to determine the size of the erase area 1 small area 5 large area The actual size o
3. load and display a new object Spot Analyzer TiVi80 3 2 Sida 29 TIVi80 Spot Analyzer 13 File Number Displays the names of the files uploaded and displayed Clicking the brown panel in front of the name permanently removes the associated file from the Project 4 3 1 TiVi80 Project window pull down menus 1 Save Project to save the TiVi80 Project 2 Close to close the TiVi80 Project window 3 Export Data to export the calculated data as ASCII code to an Excel spread sheet Two dimensional data for files and measurement number are saved in the following sheets Mean Intensity Median Intensity Mean Background Median Background Standard Deviation for Intensity Standard Deviation for Background and Area Spot Analyzer TiVi80 3 2 Sida 30
4. the Area radio button to display areas in 3D Spot Analyzer TiVi80 3 2 Sida 14 TIV180 Spot Analyzer 22 Select Export Data in the File pull down menu to save data in an Excel file Print the file name in the File Name text edit box and press the Save button This completes the GETTING STARTED scction Spot Analyzer TiVi80 3 2 Sida 15 TIVi80 Spot Analyzer 4 DETIALED DESCRIPTION 4 1 Spot Analyzer TiVi80 main window When the Spot Analyzer TiViSO is started in tool box mode from inside the TiVi700 Analyzer no photos are loaded In the Spot Analyzer TiViSO window below the photo sequence A 0001 jpg A 00026 jpg has been uploaded by clicking the Load button and then double clicking the file icon A 0001 jpg in the MNA folder H P dei Cacib amp Dipy Tiina Pevameters Erase Size Each photo is displayed in a separate sub photo panel The name and capturing date as well as the size of the actual photo TiV image and whether in zoom in Z In or zoom out Z Out mode are displayed below each photo 1 Load used for loading a new sequence of photos into the system The first six photos in a sequence are automatically uploaded 2 Show TiVi used for displaying the corresponding TiVi images instead of the photos after ROIs have been drawn Spot Analyzer TiVi80 3 2 Sida 16 TIVi8
5. 0 AIS 20 20 25 Capturing Time mi i Spot Analyzer TiVi80 3 2 Sida 9 TIVi80 Spot Analyzer 11 Select Save Chart from the File pull down menu Navigate to the 7iVidemonstration folder and print MyFirst in the Filename edit box Click the Save button The TiVi80 Chart data will now be saved under the name MyFirst_SA mat SA stands for Spot Analyzer TiVi80 Click the Close button to close the TiVi80 Chart window The TiVi80 Chart window can be opened again by clicking the Open Chart item in the File pull down menu in the Spot Analyzer TiViSO main window The data in the TiVi80 Chart window can also be loaded into the Project window together with the results from other Charts 12 Click the New Proj item in the File pull down menu in the Spot Analyzer TiViSO main window to open a new Project window im Ertha Backgreeted Barn Debi Char Lasi Coc 13 Click Load Object to load a new object into Project Navigate to the TiViSOdemonstration folder and double click the WI SA file icon to load the first object into Project The TiVi80 Project window should now look like Spot Analyzer TiVi80 3 2 Sida 10 TIVI80 Spot Analyzer Tiva bd ean AL y 14 To load five more objects into Project again click the Load Object button When the Pick a file window opens press the key board Ctr button while at the same time clicking the MyFirst SA W3
6. 0 Spot Analyzer i Setings Manua About Demno Asistert EX eot Fader Ftoi 1 Photo amp oye ae et base a ET Pe nae ea ema THs FE TELE ER AGREE SiTi HFA ore Yew Angle Caicuaie amp Dipay Thi mepe Parameters iE Top View Wane Coron 400 iI Sy i Asmak Frame Soe 2 3 Click the same button now labeled Show Photos to again display the photos 4 Click the Reset All button to again display the original photos 5 Place the mouse pointer over a specific photo A white box the dimensions of which are determined by the Set ROI Size panel edit boxes and sliders now appears over the photo and follows the mouse pointer as the mouse is moved over the photos without pressing the mouse buttons 6 Move the X Scale and Y Scale sliders until the associated edit boxes read 25 and 15 respectively These settings determine the size of the white ROI box as percentage of full scale The settings can also be altered by printing the numbers directly in the edit boxes Moving the mouse over the photo in photo panel Photo 4 should now display like Spot Analyzer TiVi80 3 2 Sida 17 TIVi80 Spot Analyzer File jarne baral About Dema Assar 7 When the mouse is positioned over the center of the bottom left erythema patch in Photo 4 click the left mouse button A zoomed in photo is displayed with the center corresponding to the mouse pointer position and the size correspon
7. 6 Manual the Spot Analyzer TiViSO on line manual 7 About Spot Analyzer TiViSO About window Spot Analyzer TiVi80 3 2 Sida 25 TIVi80 Spot Analyzer The TiVi80 Settings window allows the User to set several TiVi80 parameters These parameters are stored and saved in between sessions Background fraction 0 2 Maximum fraction Threshold fraction Intensity fraction 1 X Scale slider and edit box to set the width of the ROI box percentage of photo width Also updates the corresponding controls in the Spot Analyzer TiViSO main page 2 Y Scale slider and edit box to set the height of the ROI box percentage of photo height Also updates the corresponding controls in the Spot Analyzer TiViSO main page 3 Background fraction sets the lowest fraction of the TiV180 image as background normal skin TiVi values 4 Maximum fraction sets the fraction over above which the TiVi values are used to calculate the maximum value 5 Treshold fraction fraction value used as threshold for calculation of area extension 6 Intensity fraction fraction above which values are included for calculation of the erythema intensity Spot Analyzer TiVi80 3 2 Sida 26 TIVI180 Spot Analyzer Suppose we want to analyze the Intensity and Area of this lesion How do we proceed Displaying it ata tited angle We need to define the strength and extension of the reaction Viewing it from the si
8. Analyzer 21 Click Calculate Max to display the areas in each TiVi image representing maximal values Threshold settings from the TiVi80 Settings window Fie bing Manu About Demo Anirimt Pioi i ian a 22 Click Calculate Threshold to display the areas in each TiViimage representing values above threshold Threshold settings from the TiVi80 Settings window Spot Analyzer TiVi80 3 2 Sida 24 TIVi80 Spot Analyzer File Setting Mawai bout Dermo Amet G Feria 1 Thi 108 ara Shape Takali Bo Cikini Hix Gavcalne Threshaed RES ig PRPS oe vew ire Top View T Gide Viera 23 Click Intensity amp Area to calculate the intensity and area with associated standard deviations for each Ti1Vi80 1mage 24 Click Generate Chart to display the TiVi80 Chart with information about intensity and area for each TiVtimage 25 When the system displays TiVi images the actual TiVi value at the mouse pointer is displayed in the upper right corner of the 7iViSO Spot Analyzer TiViSO main window 4 1 1 TiVi 80 Spot Analyzer TiVi80 main window pull down menus 1 File Open Chart to open an existing TiVi80 Chart window 2 File Open Project to open an existing TiVi80 Project window 3 File New Project to open anew TiVi80 Project window 4 File Close to close the Spot Analyzer TiViSO main window 5 Settings displays the T1Vi80 Settings window see below
9. Spot Analyzer TiViSO will be a productive tool in the assessment and follow up of spot appearance following treatment with skin care products Thank you for choosing the WheelsBridge Spot Analyzer TiViSO WheelsBridge AB Spot Analyzer TiVi80 3 2 Sida 1 TIVi80 Spot Analyzer Information in this document is subject to change without notice and does not represent a commitment on the part of WheelsBridge AB No part of this manual may be reproduced or transmitted in any form or by any means electronically or mechanically including photocopying and recording for any purpose without the written permission of WheelsBridge AB Any use of application of WheelsBridge products must be performed by properly trained and qualified personnel WheelsBridge AB takes no responsibility for action taken on the basis of information derived from the use of the product described in this manual or from consequential damages in connection with furnishing performance or use of this manual Licensee s limited rights are governed by the license agreement between WheelsBridge AB and MathWorks Licensee may not modify or remove any license agreement file MarthWorks or third party that is included with the MCR Libraries MCR Library License Licensee of this Application accepts the terms of the MCR Library License The Spot Analyzer is patented and or patents are pending in several countries WheelsBridge is a registered trade mark Spot Ana
10. ViSOdemonstration folder Double click the Day 0001 jpg icon to load a sequence of photos The Spot Analyzer TiViSO main window should now look like Spot Analyzer TiVi80 3 2 Sida 4 TIVI80 Spot Analyzer Fike Settings Mangal About Dama Mahinmi Preise Pret 1 Tay OH jeg Te ee TE Poets 4 3 The four photos in the sequence are now uploaded demonstrating the initial healing process of an experimental wound 4 Adjust the X Scale and Y Scale sliders until the values in the associated edit boxes read 10 When the mouse is moved over a photo a rectangular white box is displayed Move the mouse pointer to point amp the center of the left wound spot in Photo 1 Spot Analyzer TiVi80 3 2 Sida 5 TIVI80 Spot Analyzer Tile Setinge bienal About Demo Amitan ner Pd 5 Click the left mouse button Photo I will now zoom in and display the wound at the center of the zoomed in photo File Settings Miras Shout Demo Assetant ist Phets 7 Piolo 2 Cate ab Emi Gey ez Goh eke imr Thr Re Ftoin i fm iii eg rd I i aa Be na Imi T Set AOI Sca Tee Aaga a i E E Tep view z ps O i Side View aati Calculate amp Cipy Asset 2 oki T Tre Cae qin rou Mae OR eZ Genet THA Fria fi Lard Fhetes E Samuence 1 dahon Coir Spain Erase Som 2 Spot Analyzer TiVi80 3 2 Sida 6 TIVI80 Spot Analyzer 6 Place the mouse pointer over th
11. _SA W4_SA WA5 SA and WA6_SA file icons The Pick a file window should now look like Pick a tke Leta i ie Tid Siiterorination Hgo i khim SA fr SAP ii Ehi Tiders pisser WISS k yW S a Ka S TESA w MS SA TOW S PWE SAna Mph Gina WI SAri W SAna Spot Analyzer TiVi80 3 2 Sida 11 TIVI80 Spot Analyzer 15 Click the Open button to load the selected files BE TEE Bo Fi on Tiva keen ALLS Spot Analyzer TiVi80 3 2 Sida 12 TIVI80 Spot Analyzer 17 Click the Measurement radio button to display the results with the number of measurements in each TiVi image sequence along the independent axis Fia Herter E ai HE iFa E a im iw ERE ERa Tii bari MEN 18 Click the brown panel in front of the W6 mat text to permanently remove this file from the Project The TiVi80 Project window should now look like Spot Analyzer TiVi80 3 2 Sida 13 TIVI80 Spot Analyzer 19 Check the 3D radio button to display the results in 3D mode 20 Place the mouse pointer in the center of the diagram The shape of the pointer now changes to circular Press the left mouse button and move the mouse The view angle of the 3D display now changes 21 Click
12. de Max level ae Max level e g median of 10 highest Intensity average TiVi value Discrimination level halfway between value within defined size Background level e g 20 lowest fraction Normal skin level Zero level no RBCs Size of lesion 4 2 TiVi80 Chart window The TrVi80 Chart window displays the mean or median intensity and the area of up to six TiV images Fa ET c 200 La T z T P an H Standard Dewiation 08 20 35 8 21 10 AAM 0922 10 eee 09 2310 Capturing Time i 1 Mean Intensity to display the mean intensity calculated 2 Median Intensity to display the median intensity calculated To be used instead of mean intensity if single outlier values are present Spot Analyzer TiVi80 3 2 Sida 27 TIVI180 Spot Analyzer 3 Area to display the extension of the erythema area 4 Erythema to display the erythema superimposed on the background normal skin TiVi value alone 5 Background to display the background normal skin TiVi value alone 6 Both to display the erythema value stacked on top of background see Fig above 7 Standard Deviation check to display the standard deviation bars Close to close the TiVi80 Chart window 4 1 2 TiVi 80 Chart window pull down menus 1 Save Chart to save the Chart 2 Close to close the TiVi80 Chart window 3 Export Data to exp
13. ding to the white box settings At the same time the size indicators change to the size of the photo displayed while the zoom indicator displays Z In Spot Analyzer TiVi80 3 2 Sida 18 TIVI80 Spot Analyzer Phris Prete 1 wn a pl a TIN ft HEEE MET oS ee oe See TEA 1 THH Tp r Piele4 PaT HEH gg Me zo aE I BY HA EH mia THA DPE Er TEST View Ange aicuiatz amp Opuy Tiape Paresreters 1 See Liew Aurat Ermes Size 8 To return to the original photo place the mouse pointer somewhere inside the actual photo and click the left mouse button 9 Successively point to the center of the lower left erythema patch in all photos and click the left mouse button to place all photos in zoom in mode The Spot Analyzer TiViSO main window should now look like Spot Analyzer TiVi80 3 2 Sida 19 TIVI80 Spot Analyzer 10 Click the Show TiVi button to display the TiVi images corresponding to the selected regions of interest m the photos 11 Color Span use these settings to change the color scale of the TiVi images displayed In this particular case set the Color Span to 400 The Spot Analyzer TiViSO main window should now look like Spot Analyzer TiVi80 3 2 Sida 20 TIVI80 Spot Analyzer File Settings Manual Abot Demo Asistam koni iriga Thi 15 Pons _ Pioi ARE i ATEN De ner za Rom og wer
14. e same spot m Photo 2 and repeat the procedure Repeat this procedure for all four photos The Spot Analyzer TiViSO main window should now look like Fie Settings himu About Demo Argem Ei rent Lacy Photos 7 To display the TiViimages corresponding to the zoomed in photos click the Show TiVi button The sequence of TiVitimages demonstrate how the erythema intensity and extension both are reduced as the wound heals from day one to day four Spot Analyzer TiVi80 3 2 Sida 7 TIVi80 Spot Analyzer Fie tiime Manual About Demo Ass tant Tayi po Li Sh Caro Howe TN arare Pima En FUCHS Gakuin Dipi Thiirage Parameters Ara Erase Sie 8 Click the Automatic button to calculate the intensity and area of the four individual TiVtimages The TiVi80 Chart window is now displayed showing the Mean Intensity of the Erythema Deeply re in Hean Wiensity i a F 4 a Pean inborsst EJ 20 41 42 ZIJA W 2022575 Capturing Time Spot Analyzer TiVi80 3 2 Sida 8 TIVI80 Spot Analyzer 9 Select Both and check the Standard Deviation check box to display the Erythema stacked on the Background TiVi values together with the associated standard deviations ddid 20 3740 203920 ees Capturing Time 400 PI T J0 nm T L 2 E 3 10 Click the Area button to display the area bar sequence L 204142 20 37 4
15. f the erase area is influenced also by the size of the TiVi image 17 Reset All to reset the system and again display the originally loaded photo sequence 18 Automatic clicking this button automatically calculates the intensity and area of the displayed TiVi80 images with threshold values as set in the TiVi Settings window and generates the T1Vi80 Chart to display the results 19 Manual On clicking this button opens the Manual Steps panel with buttons that takes the user to the different steps towards calculating the mtensity and area and to displaying the results in the TrVi80 Chart Spot Analyzer TiVi80 3 2 Sida 22 TIVI80 Spot Analyzer Fie Sattngn Wanu About Demo Amriant EXD teste nage j Free Phoa 1 Emka ae a HUG jog ma a r maarr Pe Be li oe i Cecais amp Dipay Tivi image Parameters Mana Ott Tuor Span 20 Click Calculate Bg to display the areas in each TiVi image used as background normal skin TiVi value Threshold settings from the TiVi80 Settings window Poets amp Alira im Ir Paa ni pa E ee a eo Apr ta Pee Ba L aE Viet Ank Cate amp Dep e Til eee Pores Tia aw 2 Spot Analyzer TiVi80 3 2 Sida 23 TIVI180 Spot
16. lyzer TiVi80 3 2 Sida 2 TIVi80 Spot Analyzer 1 INTRODUCTION Facial pots including acne appear most frequently during puberty with a varying degree of severity About 90 of the adolescent population is believed to be affected by this skin disease Numerous cosmetic products are commercially available for treatment of spots and new and more effective products are continuously released on the market To evaluate the performance of these skin care products the product candidates are generally tested using a panel of volunteers with spots of varying severity The result is assessed by naked eye observation at which both the intensity and the extension of the spot erythema are evaluated These evaluations are generally performed by a skilful dermatologist and a severity score from one to five may be used to quantify the findings Because of the unavoidable user dependent element of these naked eye inspection procedures results may vary among observers and inter laboratory comparison of data may be difficult to perform The Spot Analyzer TiViSO based on photos captured with a polarization spectroscopy camera that generates colour coded images of the red blood cell concentration in the skin streamlines the analysis process and delivers user independent results regarding both spot intensity and extension 2 OPEARATING PRINCIPLE The Spot Analyzer TiViSO operates in the following way 1 A sequence of photos of the same skin site is cap
17. ort the calculated data as ASCII code to an Excel spread sheet The following data sets are saved Mean Intensity Median Intensity Mean Background Median Background Standard Deviation for Intensity Standard Deviation for Background and Area 4 2 TiVi80 Project window In the TiVi80 Project window the results from up to twenty different Chart calculations are uploaded and displayed The TiVi80 Project window typically displays the overall result of a full test panel session Spot Analyzer TiVi80 3 2 Sida 28 TIVi80 Spot Analyzer 3 x i A Ea 8 9 Fie Humes Mean Intensity to display the mean intensities calculated Median Intensity to display the median intensties calculated To be used instead of mean intensity if single outlier values are present Area to display the extension of the erythema areas Erythema to display the erythema superimposed on the background normal skin TiVi value alone Background to display the background normal skin TiVi values alone File Number sorts the results after file number session along the waxes Measurement sorts the result after measurement number along the xaxes 2D Displays the result as two dimensional bar graphs 3D Displays the result as three dimensional bar graphs 10 Standard Deviation check to display standard deviation bars 11 Clear all click to clear the display 12 Load Object to
18. tured at different points in time 2 This sequence of photos is uploaded to the Spot Analyzer TiViSO main window 3 Specific spot are brought into focus by clicking on them using the mouse 4 The corresponding TiVi images RBC concentration maps are displayed 5 The intensity and extension of the erythema around the spots in focus are automatically calculated and displayed in the Chart window 6 Up to 20 separate spot sequences from the Chart windows can be exported to and displayed in the Project window which displays the complete results of a test panel trial In the GETTING STARTED section these features are further explained by way of an example Spot Analyzer TiVi80 3 2 Sida 3 TIVi80 Spot Analyzer 3 GETTING STARTED The basic features of the Spot Analyzer TiViSO are probably best explained by way of an example In the following example it is assumed that the photos Day 0001 jpg to Day 0004 jpg have been captured and stored in the 7iViSOdemonstration folder These photos demonstrate the initial four days healing process of experimental wounds made by a lancet on the volar side of the forearm The task is to analyze the intensity and extension of the erythema associated with these wounds in the initial wound healing phase 1 Open the Spot Analyzer TiViSO main window File Seteg Meno dhaut Deno Amtn ft ret Pico i THA kaagi Parreira Col Epi TEH Ermer Kae 2 Click the Load button navigate to the 7i
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