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PERFUSION PRESSURE MONITOR

1. lt FLOW FLOW ADJUST z ay ma je SS kult SE ri LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR Transducer Connections The following describes how to use the PM 4 with a Living Systems single vessel chamber Model CH 1 Refer to the figure below Figure 4 2 Wiring Diagram BACK OF PM 4 OUTPUTS A C POWER TRANSDUCER INPUTS P1 P2 PI lt FLOW 1 Connect the Pi transducer to the proximal inflow side of the vessel chamber 2 Connect the P2 transducer to the distal outflow side of the vessel chamber 3 Connect the Pi transducer cable to the PI TRANSDUCER INPUT on the back of the PM 4 4 Connect the P2 transducer cable to the P2 TRANSDUCER INPUT on the back of the PM 4 Transducer Connections with a PS 200 The PM 4 can be used in conjunction with the Living Svstem PS 200 Pump and Pump Servo Refer to Figure 4 3 The signal from the Pav output jack mav be used in conjunction with the Pressure Servo to maintain the vessel pressure constant Be sure both pieces of equipment are ON The Displav Control Knob on the front of the PM 4 mav now be set to read anv of the four pressures P1 P2 dP or Pay Each BNC output jack can be connected to a data LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR acquisition system such as the DATAQ Model DAQ L U interface offered by Living Systems w
2. 000e Manometer 5 PI P2 dP Py TRANSDUCER INPUTS ea E SEE ef ourtow 2 way Stopcock P1 2 way Stopcock Coupler P2 2 way Stopcock Connect the hardware as shown above in Figure 3 1 1 Connect the P1 transducer cable to the P1 jack on the back of the PM 4 2 Connect the P2 transducer cable to the P2 jack on the back of the PM 4 3 Set the Display Control Knob on the front of the PM 4 to P1 4 Open the stopcocks to air and release all pressure in the manometer so that it reads 0 00 5 The Display on the front of the PM 4 should read 0 0 If it doesn t adjust the P1 Zero Adjustment Potentiometer until the reading is 0 0 6 Set the Display Control Knob on the front of the PM 4 to P2 7 The Display on the front of the PM 4 should read 0 0 If it doesn t adjust the P2 Zero Adjustment Potentiometer until the reading is 0 0 LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR 10 11 12 Close the stopcock on the distal side of the P2 transducer and increase the manometer pressure to a steady reading slightly less than 200 mmHg as shown below Figure 3 2 P2 Proximal Stopcock Closed with Slightly Less Than 200 mmHg in System PRESSURE MONITOR MODEL PM 4 PI P2 ZERO SCALE ZERO SCALE 2 way Stopcock CLOSED Adjust the P2 Scale Adjustment Potentiometer so that the Display on the PM 4 matches the manometer pressure value For exa
3. LIVING SVSTEMS INSTRUMENTATION www livingsvs com e VT USA PERFUSION PRESSURE MONITOR USER S MANUAL DOC 237 PM 4 User s Manual Rev 1 1 Copyright 2011 All Rights Reserved Living Systems Instrumentation www livingsys com LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR THIS PAGE INTENTIONALLY LEFT BLANK TABLE OF CONTENTS Chapter Li i a 1 IMtrO CON siii iii 1 System COMPONENTS cis io A i ek io 1 Definition of TERMS iii iii kima a la 1 ChaplerZ n 2 Hardware OVervieWs uiiniiini ici A ii 2 Front Panel a iaia 2 Rear Panel ciali la dille iaia 3 Chapter Dis AA AAA AAA 4 Transducer Calibre 4 CHAP Ari 7 Operating IASTrUCIONS e ia a TE WA aiaa 7 Plumbing Eonnections i iii 7 Transducer Connections naro a aa 8 Transducer Connections with a PS 200 nn tees eee ease nn nn nt 8 Chapter 5 10 Notes and TIPS ia A 10 Pressure Transducers spiare a i 10 Specifications a ia 10 Living Systems Contact Information nr ea rr nn nn 11 CHAPTER 1 Introduction The PM 4 Perfusion Pressure Monitor is designed to monitor and record two phvsiological pressures in the range of 0 to 200 mmHg as well as their difference and their average It is ideallv suited for studies of isolated vessels that are cannulated and perfused for example using Living System s Vessel Chambers where one transducer is located proximal to the vessel and the other distal Using this configuration the di
4. fferential pressure is a measure of the perfusion pressure giving rise to flow or as a consequence of imposed flow Also the average pressure represents the mean vessel pressure when the flow resistances of the two cannulae are similar Changes in the caliber of the vessel during a perfusion experiment will usually result in an alteration of the pressures The transducers may also be located in different branches of an isolated perfused vascular bed or organ to provide pressure information or to measure pressures at two transducer locations in the absence of flow System Components e PM 4 Pressure Monitor e Two flow thru solid state pressure transducers with extension cables for connection to the PM 4 e Four BNC BNC shielded cables for recording the pressures P1 P2 dP i e P1 P2 and Pay i e P1 P2 2 e Power Cord 117 VAC or 230 VAC Definition of Terms P1 The pressure as sensed by pressure transducer P1 P2 The pressure as sensed by pressure transducer P2 dP The differential pressure or the difference between P1 and P2 Pav The mean average pressure of P1 and P2 LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR CHAPTER 2 Hardware Overview Front Panel Figure 2 1 PM 4 Front Panel Display PRESSURE MONITOR MODEMENITA Display Control Knob Power Switch mmHg LIVING SYSTEMS P1 P2 INSTRUMENTATION www livingsys com ZERO SCALE ZERO SCALE Adjustment Potentiometers Powe
5. hich allows all four pressures to be recorded simultaneously NOTE It is very important to note that the PM 4 must be left in the ON position at all times when using the PS 200 Pressure Servo If the PS 200 Pressure Servo is removed from this system the silver phone cable connecting the P2 TRANSDUCER INPUT on the back of the PM 4 to the TO PM 4 connector on the back of the PS 200 S should be disconnected before turning the PM 4 to the OFF position Figure 4 3 Wiring Diagram with PS 200 S BACK OF PM 4 BACK OF PS 200 S OUTPUTS A C POWER PI P2 dP Py ener PIN EXTERNAL IN TRANSDUCER INPUTS e O O ETA Py TRANSDUCER Mm PRESSURE OUT NORMAL TO PM 4 A C POWER lt m FLOW 1 Connect the P1 transducer to the proximal inflow side of the vessel chamber 2 Connect the P2 transducer to the distal outflow side of the vessel chamber 3 Connect the P1 transducer cable to the PI TRANSDUCER INPUT on the back of the PM 4 4 Connect the P2 transducer cable to the TRANSDUCER connector on the back of the PS 200 S 5 Using the provided silver phone cable connect the P2 TRANSDUCER INPUT on the back of the PM 4 to the TO PM 4 connector on the back of the PS 200 S CHAPTER 5 Notes and Tips Pressure Transducers Although the pressure transducers are closely matched they are not interchangeable If a new or different pressure transducers is used re calibra
6. ize W x H x D 5 7 x 2 56 x 6 89 10 PT F Pressure Transducer Pressure Range 50 300 mmHg Cable Length 48 Inflow Outflow ports Male Luer with one 1 way stopcock Living Systems Contact Information Please contact us with any questions or if hardware issues occur Please find all contact information at www livingsys com 11
7. mple if the manometer reads 198 then the digital display should also read 198 Also the voltage at the Transducer Output P2 connector which can be read with a multimeter will be 1 98 V Set the Display Control Knob on the front of the PM 4 to P1 Adjust the P1 Scale Adjustment Potentiometer so that the Display on the PM 4 matches the manometer pressure value For example if the manometer reads 198 then the digital display should also read 198 Also the voltage at the Transducer Output P1 connector which can be read with a multimeter will be 1 98 V Repeat steps 3 through 11 to ensure that the settings are accurate LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR 13 Close the two way stopcock separating the two transducers and open the stopcock on the distal end of the P2 transducer as shown below Figure 3 3 Verifying the Reading of dP PRESSURE MONITOR MODEL PM 4 Po dP U PI mmHg PI P2 D LIVING SYSTEMS 9 ZERO SCALE ZERO SCALE bi mu cio P1 P2 2 wav Stopcock CLOSED 14 Set the Displav Control Knob on the front of the PM 4 to dP The reading should be the same as P1 15 Set the Displav Control Knob on the front of the PM 4 to Pav 16 Pavis P1 P2 2 In this case P2 is zero Therefore Pay is half of P1 P1 0 2 WARNING If the PM 4 is being used in conjunction with the Pressure Servo Model PS 200 unit and only one P2 transducer is bei
8. ng used after plugging the double ended telephone cable into the lower port of the PS 200 and into the P2 port on the PM 4 it is very important that they be calibrated together and both units be left in the power ON position If you wish to use the PS 200 subsequently without the PM 4 be sure that the double ended telephone type cable is disconnected from the back of the PS 200 before turning the PM 4 off LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR CHAPTER 4 Operating Instructions It is important that the reading of P1 be greater or equal to the reading of P2 in order for the differential pressure dP P1 P2 reading and output signal be a correct positive pressure value This is done by creating flow conditions from the proximal end to the distal end of the vessel Plumbing Connections When proximal pressure to the vessel inflow cannula is being controlled and measured a common arrangement is shown in the figure below It is suggested that tubing Tygon R3603 2 4 mm O D 0 8 mm I D be used to connect the distal end of the pump tubing to the pressure transducer The lengths of the tubing going from the pump to the transducer and inflow cannula may be as long as desired but keep in mind that the longer the length the more time it will take for perfused drugs to reach the vessel Figure 4 1 Plumbing Diagram SERVO PUMP P FLOW 0 FLOW gt PRESSURE
9. r Switch Toggle switch to turn the PM 4 ON and OFF Display Displays the value of the pressure reading that has been selected using the Display Control Knob Display Control Knob The display control knob allows the user to select which pressure reading to display Pay dP P2 or Pl Adjustment Potentiometers The adjustment potentiometers allow the user to adjust the zero pressure reading ZERO and calibrate SCALE both P1 and P2 LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR Rear Panel Figure 2 2 PM 4 Back Panel Outputs OUTPUTS A C POWER Oi r 0000 seria RA P2 dP Pg fi TRANSDUCER INPUTS SERIAL 4 IN Transducer Inputs Outputs Four BNC analog output voltage jacks BNC provide 10mV mmHg signals for simultaneous recordings of P1 P2 dP and Pay Transducer Inputs Pi and P2 refer to the telephone style jacks for the pressure transducers A C Power Connector for the line cord LIVING SYSTEMS INSTRUMENTATION PM 4 PRESSURE MONITOR CHAPTER 3 Transducer Calibration It is important to note that changes in the temperature of the transducer will affect the calibration If this is critical the following calibration procedure should be performed with a heating lamp which will raise the temperature of the transducers to approximately that of the warm solution that flows through the transducers during a procedure Figure 3 1 Transducer Calibration Hardware Configuration OUTPUTS A C POWER
10. tion of the svstem is required Follow the instructions in Chapter 3 to calibrate the transducers In addition to phvsical damage dropping etc pressure transducers can be damaged by excessive pressures above 5000 mmHg As a result the pressure readings may not be correct and erroneous readings will be observed In some situations a broken transducer may have solution dripping or spraying from the tiny air pressure equilibration hole in the center of the flat cable about 22 cm from the transducer WARNING Pressure transducers should never be immersed in liquid This may cause severe damage In rare situations it has been observed that the pressure transducer is susceptible to ambient radio frequency interference such as signals from cordless telephone transmitters These erroneous signals can produce small random pressure jumps If this problem is occurring it is advisable to wrap the transducer in aluminum foil and connect the foil to ground with a wire lead and clip The PM 4 must be grounded and connected to a three pin power source It is not meant for use in environments where explosions may occur Specifications PM 4 Pressure Monitor Power 100 120 VAC 60 Hz 200 240 VAC 50 Hz Pressure range 0 200 mmHg Range 0 1 mmHg resolution 1 linearity 3 samples s 10 mV mmHg Analog Signal Outputs lt 15 ms response time 1 linearity Weight 0 3 kg 0 66 Ibs 14 5 cm x 6 5 cm x 17 5 cm S

PERFUSION PRESSURE MONITOR

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