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chemical hygiene plan and hazardous materials

1. Section 2 Type of SOP CI Process L Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A GHS Classification Flammable or Combustible Liquid depending on the type of flammable liquid other hazards such as toxicity often apply as well Molecular Formula N A Form physical state Liquid Color N A Flammable Combustible Liquids Date 7 1 2013 PURDUE UNIVERSITY Boiling Point N A Flash Point N A Lower Explosive Limit N A Upper Explosive Limit N A Relative Vapor Density N A Uses Many laboratory applications require the use of flammable and or combustible liquids Uses include applications such as cleaning preparing solutions organic synthesis spectrometry techniques and many more Examples of commonly used flammable and combustible liquids in the laboratory include Acetone Hexane Tetrahydrofuran Diethyl ether and Kerosene The safe use of flammable and combustible liquids is fundamental to any laboratory management system Important Definitions e Flammable Liquid Liquids having a flash point below 38 C 100 F e Combustible Liquid Liquids having a flash point at or above 38 C 100 F and no greater than 93 C 200 F e Flash Point The minimum temperature at which vapors are formed on the surface of a substance in sufficient quantity to ignite when exposed to an ignition source Fire Point The
2. have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Bromine Date 2 17 2014 PURDUE UNIVERSITY Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Bromine Date 2 17 2014 PURDUE UNIVERSITY Standard Operating Procedure Corrosives This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical
3. Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Methyltrichlorosilane is not permitted to be poured down the drain Section 12 Safety Data Sheet SDS A current methyltrichlorosilane SDS copy must be made available to all personnel working in the laboratory at all times www sigmaaldrich com MSDS MSDS PleaseWaitMSDSPage do language amp country US amp brand ALDRICH amp roductNumber M85301 amp PageToGoToURL http www sigmaaldrich com catalog product aldrich m85301 lan en amp region US Methyltrichlorosilane Date 12 13 2013 PURDUE UNIVERSITY Section 13 Protocol Procedure Additional lab protocol may be added here Click here to enter text N
4. e REM SDS Search http www purdue edu rem ih msds htm e Siri MSDS Index http hazard com msds e Sigma Aldrich Product Search http www sigmaaldrich com united states html 5 5 Chemical Labeling Requirements Every chemical container present in the laboratory whether hazardous or not must be properly labeled All secondary Te sms aroan Peon 5 1 UN2014 PG for 15 minus u contact chemical containers e g wash bottles beakers flasks sample kann nie yaar vials etc must also be properly labeled Avoid using O an ieee chemical Carbon Dioxide chemical foam abbreviations chemical formulae or structure unless there is mann a complete and up to date legend e g MeOH Methanol eg CORROSIVE Do not add any other o product CODE No contamination from any source prominently posted in the lab Most chemicals come with a manufacturer label that contains all of the necessary 32 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan information so care should be taken to not damage or remove these labels It is recommended that each bottle also be dated when received and when opened to assist in determining which chemicals are expired and require proper disposal Detailed information and strategies for the labeling of research samples is discussed in Section 5 9 of the CHP These same strategies can be used when labeling secondary chemic
5. sssssscccsssssccccssssssccccssssceccesssceccccessecsceessseees 94 Tab 1 Specific Standard Operating Procedures ssssesssssssssoosssssesssesesssesscsssssosssssceseseeesess 97 Tab 2 Lab Specific Protocols Requirements Rules ssscccssseccsseeccsseccnsseecsscccsesceeeeees 98 Tab 3 Lab Specific Hazard Assessments cccssssceccccssssccccssssceccecsssececcssssceccacsseeceenesseeeeees 99 iv The official version of this document will only be maintained online ANSI ASTM CFR CHO CHP CLSC DOT EHS EPA GFCI GHS HBr HF HEPA HPLC IBC IDEM ISP LC LC50 LD50 LEL LSC MSDS NFPA OSHA PCB PHS PI PPE RCRA rDNA REM RSC SAA SDS SOP UEL CHP Document Acronyms List CHP Document Acronyms List American National Standards Institute American Society of Testing and Materials Code of Federal Regulations Chemical Hygiene Officer Chemical Hygiene Plan Chemical and Laboratory Safety Committee Department of Transportation Environmental Health and Safety Environmental Protection Agency Ground Fault Circuit Interrupter Globally Harmonized System of Classification and Labeling of Chemicals Hydrogen Bromide Hydrofluoric Acid High Efficiency Particulate Air High Performance Liquid Chromatography Institutional Biosafety Committee Indiana Department of Environmental Management Integrated Safety Plan Liquid Chromatography Lethal Concentration 50 Lethal Dose 50 Lower Explosive Limit Laser Sa
6. The regulator must be replaced with a safety cap when the cylinder is not in use The safety cap must be in place when a gas cylinder is moved For large gas cylinders gt 27 inches an approved gas cylinder cart should be used The cylinder must be strapped to the cart and the protective cap must be in place before moving the cylinder A cylinder should never be moved or transported without the protective cap The proper way to move a large gas cylinder is illustrated in Figure 5 7 Never dispense from a cylinder if it is on a gas cylinder cart 41 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Figure 5 7 Gas Cylinder Cart A few compressed gas cylinders have a shelf life and can become more hazardous as time goes on It is extremely important that these chemicals are identified and managed properly If any time sensitive gases are found to be past the manufacturer s expiration date they must be submitted to REM for hazardous waste disposal immediately The following is a list of time sensitive compressed gases Hydrogen Fluoride anhydrous e Hydrogen Bromide anhydrous e Hydrogen Sulfide anhydrous e Hydrogen Cyanide anhydrous e Hydrogen Chloride anhydrous The compressed gases listed above have a shelf life provided by the manufacturer that must be strictly followed There have been numerous incidents involving these compounds related to storage past the ex
7. as listing all of the chemicals that makeup the waste If a chemical is found in the laboratory and the composition is unknown it should be assumed to be hazardous and labeled as Hazardous Waste awaiting proper characterization Figure 7 1 Purdue University Hazardous Waste Label 70 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 3 Waste Storage Requirements Hazardous waste containers in Purdue laboratories are stored in satellite accumulation area SAA SAAs are used to manage hazardous waste in laboratories and shops because doing so provides safe and effective means to accumulate hazardous waste before removal by REM Additionally SAAs provide the least restrictive regulatory option for the accumulation and storage of hazardous waste containers The following SAA rules must be followed at all times when managing hazardous waste in a laboratory e All waste must be stored in containers e Containers must be in good condition and compatible with the waste they contain no corrosive waste in metal containers e Containers must be kept closed at all times except when adding or removing waste e Containers must be labeled or clearly marked with words that describe the contents of the waste e g liquid chromatography waste and the words Hazardous Waste e Containers must be stored at or near the point of generation and under the control of the ge
8. Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Any quantity of Bromine is not permitted to be poured down the drain Section 12 Safety Data Sheet SDS A current copy of the SDS for Bromine must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additional lab protocol may be added here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with Bromine designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year
9. Not In Use Cylinders Figure 5 4 In Use Cylinders 5 7 4 Reactive Materials Storage Reactive materials include explosives pyrophorics self heating and self reacting compounds and water reactives Many reactive materials are also toxic and are dissolved or immersed in a flammable solvent e g lithium alkyl compounds dissolved in diethyl ether sodium metal immersed in mineral oil Other common hazards often associated with reactive chemicals include corrosivity teratogenicity or organic peroxide formation The following guidelines for storing reactive materials must be followed in all laboratories e The amount of reactive materials stored in the lab must be kept to a minimum Any expired or unnecessary reactive materials must be properly disposed of as hazardous waste e All reactive materials must be clearly labeled with the original manufacturer s label which should have the chemical name hazard labels and pictograms The label should not be defaced in any way e All reactive materials should be placed into secondary containment as a best management practice e Suitable storage locations for reactive materials include inert gas filled desiccators or glove boxes flammable storage cabinets that do not contain aqueous or other incompatible chemicals or intrinsically safe refrigerators or freezers that also do not contain aqueous or other incompatible chemicals If possible store all reactive chemicals in a small flammable cabinet d
10. UNIVERSITY Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Potassium Cyanide Date 11 26 2013 PURDUE UNIVERSITY Standard Operating Procedure Pyrophoric Materials This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process L Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A GHS Classification Pyrophoric Water Reactiv
11. s label with the chemical name hazard labels and pictograms should not be defaced or covered 5 7 8 Refrigerators and Freezers Chemical Storage A number of general precautions need to be taken when storing chemicals in refrigerators and or freezers in the laboratory When working with freezers or refrigerators the following procedures must be followed e Domestic refrigerators or freezers must never be used to store flammable liquids Flammable liquids are only allowed to be stored in refrigerators or freezers that are designed for flammable materials most refrigerators are not intended for flammable storage e Lab refrigerators or freezers must never be used to store food or beverages for consumption Lab refrigerators freezers should be posted with a sign that states No Food or Drink e All chemicals stored in a refrigerator or freezer must be labeled e Ensure that the chemicals stored in a refrigerator or freezer is compatible with each other For example do not store an oxidizer such as hydrogen peroxide in a refrigerator with organic chemicals e There must not be any open chemicals in a refrigerator or freezer All containers must be completely sealed or capped and safely stored e Chemicals should be allowed to warm to room temperature before sealing to prevent pressure buildup 40 The official version of this document will only be maintained online 5 8 Chapter 5 Laboratory Management Plan e Shelves in
12. Chemical Properties CAS N A GHS Classification Corrosive Molecular Formula N A Form physical state Liquid or Solid Color N A Boiling Point N A Corrosives Date 10 1 2013 PURDUE UNIVERSITY Flash Point N A Lower Explosive Limit N A Upper Explosive Limit N A Relative Vapor Density N A Uses Acid and bases are common laboratory chemicals that are also used in plastic production cleaning and descaling agents detergents fertilizers oil refining and paper manufacturing among others Sulfuric acid is the electrolyte in lead acid batteries Aqueous potassium hydroxide is employed as the electrolyte in alkaline batteries based on nickel cadmium and manganese dioxide zinc Most corrosive materials are hygroscopic meaning they readily absorb moisture in air Important Definitions e Acid Any chemical compound which when dissolved in water gives a solution with a pH of less than 7 0 e Mineral Acid A compound having atoms of hydrogen identifying nonmetal typically chlorine sulfur or phosphorus and maybe oxygen Sulfuric acid H2SO and Hydrochloric acid HCI are examples of mineral acids e Organic Acid An organic compound with acidic properties Generally Organic acids are also flammable Acetic acid CHCOOH and Formic acid HCOOH are examples of organic acids e Base Any chemical compound which when dissolved in water gives a solution with a pH of greater than 7 0 Section 4 Potentia
13. O O structure and may be considered a derivative of hydrogen peroxide where one or both of the hydrogen atoms have been replaced by organic radicals e Oxidizer A liquid or solid while in itself is not necessarily combustible may generally by yielding oxygen cause or contribute to the combustion of other material e Pyrophoric Substance also called Spontaneously Combustible A liquid or solid that even in small quantities and without an external ignition source can ignite after coming in contact with the air 16 The official version of this document will only be maintained online Chapter 2 Classes of Hazardous Chemicals e Self Heating Substance A liquid or solid other than a pyrophoric substance which by reaction with air and without energy supply is liable to self heat e Self Reactive Substance A liquid or solid that is liable to undergo strong exothermic thermal decomposition even without participation of oxygen air e Water Reactive Substance A liquid or solid that reacts violently with water to produce a flammable or toxic gas or other hazardous conditions 3 2 Health Hazards A chemical is a health hazard if there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed employees Each health hazard is briefly defined below Refer to Appendix B section B 2 for detailed information on
14. contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with Benzene designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Benzene Date 8 19 2013 PURDUE UNIVERSITY Standard Operating Procedure Bromine This is an SOP template and is not complete
15. immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Hydrogen Peroxide Date 7 12 2013 PURDUE UNIVERSITY Section 8 Special Handling and Storage Requirements Avoid contact with skin eyes and clothing Avoid inhalation or ingestion Hydrogen peroxide solutions greater than 10 should be kept in their original container with the manufacturer cap that allows the container to vent oxygen while in storage as shown in the figure to the right Do not store hydrogen peroxide solutions greater than 10 in glass containers only plastic containers should be used for storage Keep container tightly closed in a dry and well ventilated area Opened containers must be carefully resealed and kept upright to prevent leakage Recommended storage temperature is 2 8 C Store away from combustible materials Do not store on wooden shelves Store away from all organic comp
16. offices or restrooms Each department is responsible for providing laundry services as needed to maintain the hygiene of laboratory coats They may not be cleaned by staff members at private residences or public laundry facilities Alternatives to laundering lab coats include routinely purchasing new lab coats for employees to replace contaminated lab coats or using disposable lab coats 6 4 7 Foot Protection Closed toe shoes must be worn at all times when in the laboratory open toe shoes and or sandals are not permitted in any circumstance Each affected employee must wear protective footwear when working in areas where there is a high risk of objects falling on or rolling across the foot piercing the sole and where the feet are exposed to electrical or chemical hazards If there is a high risk of chemical contamination to the foot e g cleaning up a chemical spill on the floor then chemical resistant booties may need to worn as well Minimum PPE Requirements for Support Staff and Visitors Support staff e g custodians maintenance workers and visitors often must enter laboratories to perform routine tasks such as maintenance or take a tour of the lab These individuals are present in the laboratory but are not performing work with or directly adjacent to any work with hazardous chemicals To be present in the laboratory the minimum PPE requirements include safety glasses long pants and closed toe shoe If additional
17. the detailed instruction and guidelines for acceptable sink disposal is provided in the Purdue University Hazardous Waste Disposal Guidelines Chapter 5 6 Appendix E and Appendix F http www purdue edu rem home booklets hwdg pdf Please contact REM at 765 49 40121 for further information regarding non hazardous chemical waste disposal 7 8 Sharps Waste Sharps are items capable of puncturing cutting or abrading the skin such as glass or plastic pipettes broken glass test tubes petri dishes razor blades needles and syringes with needles Sharps waste contaminated with hazardous chemicals must be placed into puncture resistant containers e g sharps container plastic or metal container with lid and properly labeled as detailed in Chapter 4 of the Purdue University Hazardous Waste Guidelines All chemically contaminated waste should be inventoried on a Hazardous Materials Pickup Request Form and sent to REM for proper disposal Clean uncontaminated broken glassware and plastic sharps should be placed in a corrugated cardboard box or other strong disposable container Do not exceed 20 pounds When ready for disposal the box should be taped shut and prominently labeled as Sharp Objects Glass Discard or similar wording The Safe for Disposal label Figure 7 5 should also be affixed to the outside of the container Contact your Physical Facilities Building Services department for specific non hazardous waste disposal ins
18. 11 Waste Disposal Procedures Label Waste When possible do not mix oxidizer waste streams with flammable or combustible waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm No oxidizing liquids or solids are permitted to be poured down the drain Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific oxidizing liquid or solid being used must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additional lab protocol may be added here e Usein the smallest practical quantities for the experiment being performed e Work must be conducted in a chemical fume hood if the chemical is irritating to the eyes or respiratory system and or is toxic by inhalation Container
19. 15 minutes and consult a physician Seek immediate medical attention and continue eye rinse during transport to hospital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek medical attention immediately Section 8 Special Handling and Storage Requirements Precautions for safe handling Pyrophoric use extreme care when handling Only handle under inert gas use a glove box if possible Do not expose to air Avoid contact with skin and eyes and inhalation A dry run of the experiment should be performed using low hazard materials such as water or an organic solvent Never work with lithium alkyl compounds alone Conduct the procedure only after a supervisor has observed the user performing the proper technique unassisted All glassware used for lithium alkyl compounds should be oven dried and free of moisture Keep away from sources of ignition Avoid heat and shock or friction when handling Secure all lithium alkyl containers to a stand Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances The amount of pyrophoric materials stored should be kept at a minimum Any expired or unnecessary reactive materials should be properly disposed of as hazardous waste All pyrophoric materials should be clearly labeled with the original manufacturer s label which should have the chemical nam
20. 64 6 4 5 Hand Proteetion san ae een 64 6 4 6 Body Protection sans lose T aa 65 6 4 7 FOOL Protection ars asia a ah a e i aeii 66 6 5 Minimum PPE Requirements for Support Staff and Visitors ueeeeeneneeesensennenennnnnnennennnnn 66 6 6 PPE Training Requirements ueseeseseeseennnensnnsenennnnnnnennnnnennnnnnnnnensnnnennnnnnnnnensnsnnnnnnnsnsn nenn 66 6 7 Injuries Illnesses and Medical Examinations cc cccccccecesssssssceeeceeeesssesseeseeeeeesseesees 67 Chapter 7 Hazardous Waste Manageme nt sssssccccsssscecccsssssccccesssceccecssseeeccessscsccesseees 69 2 1 WATHOGUCUON ssion A A E E EAT RE EES 69 7 2 Waste Identification and Labeling ccccccccccccecsssssscececeeeceesssseaeceeeeseessessaeeeeeesesseeees 70 7 3 Waste Storage Requirements ccsecscccececesneseeescecececeaeeueeescececessaueuseeseeeeeessaseuaeeeeeseeeea 71 7 4 Naste Contalners ie nee ein ern Rule entleeren tin Uaddeeneteedtes 72 7 5 Waste Disposal Procedures sensie ana dss cabene de Vise lis oes Biennale 74 7 6 Unknown Chemical Waste ac o css ee ennai akin ate 75 7 6 1 Labeling Unknown ChemMicals cccccccccsssssssecececeeecsesenseaeceeeeeeessesssaeeeseeeeseseseeseaeess 75 7 6 2 Identifying Unknown Chemicals cccccccesessscececeeessessssesececeeecessessasseeeeeeessessssnaeess 75 7 6 3 Removing Unknown Chemicals from the Work Area c cccccccccessessssceceeeeesessssseaeees 76 7 6 4 Preventing Unk
21. 8 Special Handling and Storage Requirements e Do not over purchase only purchase what can be safely stored in the laboratory Avoid contact with skin and eyes Avoid inhalation of vapor or mist Always use inside a chemical fume hood Do not allow water to get into the container because of violent reaction Note In case you need to dilute the concentration of Sulfuric acid always add the acid to water Do not use with metal spatula or other metal items Use in the smallest practical quantities for the experiment being performed Keep container upright amp tightly closed in a dry and well ventilated place Containers which are opened must be carefully resealed and kept upright to prevent leakage Store in original container Do not store Sulfuric acid in metal containers Sulfuric acid must be segregated from incompatible materials Sulfuric Acid is incompatible with metals oxidizing agents reducing agents bases Acrylonitrile azides cyanides chlorates finely powdered metals nitrates perchlorates permanganates Epichlorohydrin Aniline carbides fulminates halides picrates organic materials Zinc salts and flammable liquids Containers should be labeled appropriately the original manufacturer s label is acceptable Label should indicate the name of the chemical s in the container Avoid using chemical abbreviations acceptable if a legend is present in the lab and formulae Transport all corrosives in secondary containment suc
22. Acetone 50 Benzene 10 Dichloromethane 20 Acetic Acid 10 Water 10 Trace Silver I have made a good faith effort to minimize my waste generation and select the best waste management method that is available to me and that can afford Amount Solid Chemical Description List Each Container Somber or Ssontelner STWERTE Bern maula Container Aerosol m 4 Figure 7 6 Hazardous Materials Pickup Request 74 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 6 Unknown Chemical Waste Unknown chemicals are a serious problem in laboratories Mysterious chemicals are often stored in labs for years before lab personnel notice the unidentified items However steps can be taken to assist with proper management of unknowns Unknown chemicals must be properly identified according to hazard class before proper disposal The hazards that should be noted include corrosive flammable oxidizer reactive toxic and radioactive The following subsections describe in detail how to properly manage unknown chemicals 7 6 1 Labeling Unknown Chemicals Until the unknown chemical can be properly identified by either lab staff or REM the container should be labeled with a Hazardous Waste Disposal Tag The following information should be written on the label Unknown hazardous chemical awaiting proper characterization by REM as illustrated in Figure 7 7 Fi
23. Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 7664 39 3 GHS Classification Acutely Toxic Corrosive Molecular Formula HF Form physical state Liquid Color Colorless Hydrofluoric Acid HF Date 2 1 2013 PURDUE UNIVERSITY Boiling point Not applicable Uses HF is primarily used to etch glass The strongest concentration typically seen from chemical manufacturers is a 48 aqueous solution A 5 to 9 hydrofluoric acid gel is also commonly used to etch all ceramic dental restorations to improve bonding For similar reasons dilute hydrofluoric acid is a component of household rust stain remover and in car washes in wheel cleaner compounds Because of its ability to dissolve iron oxides as well as silica based contaminants hydrofluoric acid is used in pre commissioning boilers that produce high pressure steam Because of its ability to dissolve oxides hydrofluoric acid is useful for dissolving rock samples usually powdered prior to analysis The ability of hydrofluoric acid to dissolve metal oxides is the basis of several applications It removes oxide impurities from stainless steel a process called pickling and silicon wafers in the semiconductor industry Section 4 Potential Hazards Hydrofluoric acid HF is a highly corrosive liquid and is a cont
24. Chapter 6 of the CHP Laboratory Supervisors must ensure that all lab employees receive documented PPE training before any work with hazardous materials occurs Document PPE training using the Certification of Training form Appendix B of the Purdue University Personal Protective Equipment PPE Policy http www purdue edu rem home booklets PPEPolicy pdf or get it directly from http www purdue edu rem home forms CertT pdf Each lab employee must be trained to know at least the following e When PPE is necessary e What PPE is necessary e How to properly don doff adjust and wear PPE e The limitations of the PPE e The proper care maintenance and useful life of PPE Each affected employee must demonstrate an understanding of the training provided and the ability to use the PPE properly before performing any work requiring the use of PPE 9 3 SOP Training As discussed in Chapter 4 of the CHP SOPs are written instructions that detail the steps that will be performed during a given procedure and include information about potential hazards and how these hazards will be mitigated SOPs must be developed for all high hazard tasks that are performed in the lab which is defined as work with e Explosives e Water reactive pyrophoric self heating or self reactive chemicals e Particularly hazardous substances which includes carcinogens reproductive toxins and acutely toxic substances e Compressed gases e Work involving more tha
25. Department Chemistry Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP Building Room Number Section 2 Type of SOP CI Process Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A GHS Classification Flammable Corrosive Molecular Formula N A Form physical state Liquid Color N A Boiling Point N A Base Baths Date 1 17 2014 PURDUE UNIVERSITY Uses Base baths are highly concentrated alcoholic hydroxide solutions used to clean glassware after use The solutions consist of Ethanol or Isopropanol and Sodium or Potassium hydroxide The glassware is cleaned by chemically dissolving contaminated surfaces Section 4 Potential Hazards Base baths are flammable and corrosive They may be harmful if inhaled ingested or absorbed through the skin Inhalation may cause irritation to the respiratory tract with burning pain in the nose and throat coughing wheezing shortness of breath and pulmonary edema It is destructive to the tissue of the mucous membranes and upper respiratory tra
26. Do not store in the top most shelf of the storage cabinet In general do not store chemicals at or above eye level e Ensure the container is tightly closed at all times Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and begin first aid procedures Section 7 immediately Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and begin first aid procedures Section 7 immediately Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Nitric Acid Date 7 2 2013 PURDUE UNIVERSITY Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels fre
27. Hoods une e eA REETA EA EER 19 4 2 2 lt GlOVe BOXe8 en eo 22 4 2 3 Laminar Flow Clean Bence vz 2 Gala 23 4 2 4 Biological Safety Cabinets ccccccccccccecssssssseeeceeecessesseaececeeeeessessaeseseeseseneseesaaeess 23 4 2 5 Safety Showers and Eyewash Stations ccccccccccccccsssessssecececeeessesssssaeeeeeeseseseeseaeess 24 22 6 Fire EXtinguishers 2 0 2 2 28er A odds sue RS 25 Al2 7 EWE DO ONS E A E E E savas dasiesadeadsateve ica chegeks EE 25 4 3 Administrative Control Ara era 26 4 3 1 Standard Operating Procedures eueeeneneesnensnnsnennnnnnnennnnnsnennnnnnnnnsnnnsnnnnnnnnnnnennn nenn 26 4 3 2 Required Laboratory Postings ueeeeeneneennennnnenennnnnnnennnnnnnennnnnnnnnsnnnsnnnnnnnnnnnennsn nenn 27 4 4 Personal Protective Equipment PPEl un seen 28 Chapter 5 Laboratory Management Plan ccccssssccccssssceccccssseccccesssceccecssceesecesseseceesseeees 29 i The official version of this document will only be maintained online Table of Contents 5 1 Laboratory Safety Guidelines cccccccssssscececeeecsesesseseeeceesesesesseaeeeeeesesssesssaeseeeesesseeeeea 29 5 1 1 Laboratory Safety Questions aesesenensssssnnnsnsnenennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnenssn nennen 29 5 1 2 General Laboratory Safety Rules uuusssseesessnsssnsnnnsnenennnnnnnnnnnnnsnennnnnnennennnsn nennen 30 5 2 Housekeeping un senstersiens er ernennen renearshniisiniegtelikens 31 5 3 Ch mical
28. If inhaled Move into the fresh air immediately and seek immediate medical attention If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Seek immediate medical attention In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Section 8 Special Handling and Storage Requirements e Do not over purchase only purchase what can be safely stored in the laboratory e Any expired or unnecessary Sodium azide should be properly disposed of as hazardous waste e Pure Sodium azide should be clearly labeled with the original manufacturer s label which should have the chemical name hazard labels and pictograms The label should not be defaced in any way Make stock solutions of 10 if possible to minimize potential accidents Sodium Azide Date 3 27 2014 PURDUE UNIVERSITY e Whenever handling pure Sodium azide powder or concentrated solutions of 210 that may require assistance in case of a spill or ac
29. Inventories a2 He 32 54 Safety Data Sheets ea aan Ra RR 32 5 5 Chemical Labeling Requirements cccscscccececscessesseceeeceeecseseseeaeceeeesesseessaeeeeeesesseesees 32 5 6 Chemical Segregation srar aneia a Aa EE EA E E aaaea 33 5 7 Chemical Storage Requirements osuisi eE E i E aaia 34 57 1 General Chemical Storage 32a a Aa 34 5 7 2 Flammable Liquids Storage cccccccccccscssssssnsececeeeeessesenseseeeseeseessesssaeeeeeesesseessesaaeeas 35 5 7 3 Compressed Gases Storage ccccccccccccscssssssnsececeesesseesesaeaeseceeecessesssaeeeeeeeessseseeseaeess 36 5 7 4 Reactive Materials Storage cccccccccccccssssssssececececessessseseeeseessessessaeeeseeeessnessssnaeess 37 5 7 5 Acutely Toxic Materials Storag no aAA E Saat 38 5 7 6 Corrosive Materials Storage ccccccccccccccsssssssseseceeeesssesseseseeeesesssessaeeeseesessneseesnaeess 39 5 7 7 Oxidizers and Organic Peroxide Storage ccccssssccccsssseceesssseeecessseaeceessseeseesseeeeees 39 5 7 8 Refrigerators and Freezers Chemical Storage ccccccsssccccsssscceceessneeeecssseeeeseseeeeees 40 5 8 Compressed Gas Cylinder Safety nrepati areeni ret u EEA E SEa 41 5 9 Cryogenic Liquids Safety cccccccccccccsssessnsecececeesceesssseseseceescssseseaeseeeeeeessesssaseeeeeseseeeeea 43 5 10 Nanoparticle Sat Oty sats Nasen 44 5 11 Sharps Handling Safety ccccccscccccecessessnsececeeeceeseseaeseceeeesseeseeaeeeeeeseessesseasaee
30. Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 1239 45 8 GHS Classification Acute toxicity Inhalation Category 3 Germ cell mutagenicity Category 2 Acute Toxicity Oral LD50 rat 1 503 2 177mg kg Inhalation LC50 rat 1h 11 8 134 mg L Ethidium Bromide Date 7 19 2013 PURDUE UNIVERSITY Subcutaneous LD50 mouse 110 mg kg Intravenous LD50 rat 16 mg kg Uses Ethidium bromide EtBr is an intercalating agent commonly used as a fluorescent tag for molecular biology techniques such as agarose gel electrophoresis Section 4 Potential Hazards Ethidium bromide is classified by OSHA as highly toxic by inhalation harmful by ingestion and a mutagen Avoid breathing dust fumes gas vapor mist or spray Toxic if inhaled Suspected of causing genetic defects gt Section 5 Personal Protective Equipment PPE Respirator Protection Under normal working conditions where ethidium bromide is in very low concentrations in agarose gel or in an aqueous solution respiratory protection is not required However all ethidium bromide powder pure product must be handled in a properly fun
31. PPE is required or if other unique safety requirements must be followed it is the lab personnel s responsibility to notify support staff and or visitors of the additional requirements PPE Training Requirements Laboratory Supervisors must ensure that all employees receive PPE training before any work with hazardous materials occurs This training must be documented Document PPE training 66 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy using Certification of Training form Appendix B of the Purdue University Personal Protective Equipment PPE Policy http www purdue edu rem home booklets PPEPolicy pdf or access it directly from http www purdue edu rem home forms CertT pdf Each lab employee must be trained to know at least the following e When PPE is necessary e What PPE is necessary e How to properly don doff adjust and wear PPE e The limitations of the PPE and e The proper care maintenance and useful life of PPE Each affected employee must demonstrate an understanding of the training provided and the ability to use the PPE properly before performing any work requiring the use of PPE When the supervisor has reason to believe that an affected employee who has already been trained does not have the understanding and skill required e g employee is seen handling hazardous materials without wearing proper PPE then the supervisor must ensure the e
32. Procedures Label Waste Corrosive waste cannot be disposed of down the drain When possible do not mix acidic and basic waste streams Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm No oxidizing liquids or solids are permitted to be poured down the drain Corrosives Date 10 1 2013 PURDUE UNIVERSITY Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific corrosive chemical being used must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additional lab protocol may be added here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Pri
33. See Appendix C for a list of commonly found organic solvents that potentially form organic peroxides 35 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan u Fo amp JUSTRITE Figure 5 1 Flammable Storage Cabinet Figure 5 2 Flammable Safety Can 5 7 3 Compressed Gases Storage Compressed gases are defined as gases that are contained in a receptacle at a pressure not less than 280 kPA at 20 C or as a refrigerated liquid The following guidelines for storing compressed gases must be followed in all laboratories e Compressed gas cylinders cylinders must be stored in a secure well ventilated location and in an upright position at all times e All cylinders should be handled as if full and should never be completely emptied e Cylinders that are not in use meaning that the cap is on must be secured and have the safety cap Multiple cylinders may be secured together gang chained only if they are capped not in use Only capped cylinders can be secured with a single restraining device gang chained as shown in Figure 5 3 e Cylinders that are in use meaning there is a regulator attached must be individually secured by a chain or strap as shown in Figure 5 4 Cylinder valves and regulators should be protected from impact or damage 36 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Figure 5 3
34. acid Always use glass preferably Pyrex containers Piranha will melt plastics Piranha solution should be used freshly prepared due to the self decomposition of hydrogen peroxide Do not maintain a stock solution of the Piranha mixture Prepare small amounts of solution to be used for each application Mix the solution in a fume hood with the sash between you and the solution The solution may be mixed before application or directly applied to the material applying the Sulfuric acid first followed by the peroxide When preparing the piranha solution always add the peroxide to the acid Hydrogen peroxide concentrations should be kept below 30 and should never exceed 50 Piranha solution should never be left unattended if hot Once finished allow the hot piranha solution to cool in an open container Once cooled down put the waste solution in a container with a vented cap Examples of vented caps are shown in Figure 1 on the right Submit the waste solution as soon as possible to REM on a Hazardous Materials Pickup Request from Never seal containers containing Piranha solution Avoid using airtight containers as pressure can build up inside of them Never store piranha solutions Oxygen given off during the self decomposition as well as the oxidation products of organic compounds can cause the container to rupture Adding any acids or bases to piranha or spraying it with water will accelerate the reaction Mixing hot piranha
35. all laboratory personnel Each laboratory worker must be familiar with the contents of the CHP and the procedures for obtaining additional safety information needed to perform their duties safely 1 1 Purpose Purdue University is committed to providing a healthy and safe work environment for the campus community The Purdue University CHP establishes a formal written program for protecting laboratory personnel against health and safety hazards associated with exposure to hazardous chemicals and must be made available to all employees working with hazardous chemicals in a laboratory setting The CHP describes the proper use and handling procedures to be followed by faculty staff and all other personnel working with hazardous chemicals in laboratory settings 1 2 Scope The CHP applies to all laboratories that use store or handle hazardous chemicals and all personnel who work in these facilities The information presented in the CHP represents best practices and provides a broad overview of the information necessary for the safe operation of laboratories that utilize hazardous chemicals Laboratory use of hazardous chemicals is defined as handling or use of such chemicals in which all of the following conditions are met 1 Chemical manipulations are carried out on a laboratory scale Multiple chemical procedures or chemicals are used The procedures involved are not part of a production process nor in any way simulate a production process an
36. and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of alkali metals must be conducted in an inert atmosphere use of a glove box is recommended The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Seek medical attention immediately In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Seek medical attention immediately In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention and continue eye rinse during transport to ho
37. and cables must be protected from accidental damage Sharp corners and projections are to be avoided Flexible cords and cables must be protected from damage when they pass through doorways or other pinch points 58 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 18 4 Wet or Damp Locations Work in wet or damp work locations i e areas surrounded or near water or other liquids should not be performed unless it is absolutely critical Electrical work should be postponed until the liquid can be cleaned up The following special precautions must be incorporated while performing work in damp locations e Only use electrical cords that have GFCls e Place a dry barrier over any wet or damp work surface e Remove standing water before beginning work Work is prohibited in areas where there is standing water e Donot use electrical extension cords in wet or damp locations and e Keep electrical cords away from standing water 59 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy Chapter 6 Laboratory PPE Policy 6 1 Purpose The purpose of this Laboratory Personal Protective Equipment PPE Policy is to ensure that all Purdue lab employees are aware of the PPE requirements and procedures to adequately protect themselves against chemical radiological biological or mechanical hazards This policy has been prepared in acc
38. approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician Nitric Acid Date 7 2 2013 PURDUE UNIVERSITY In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Section 8 Special Handling and Storage Requirements Avoid contact with skin eyes and clothing Keep container tightly closed in a dry and well ventilated area If possible store in corrosive acid lab storage cabinet within a secondary containment nalgene polypropylene tray or tub Store in original container away from direct sunlight Avoid contact with alkali metals reducing agents cyanides aldehydes powdered metals ammonia and acetic anhydride and all organic materials including organic solvents
39. at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and begin first aid procedures Section 7 immediately Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and begin first aid procedures Section 7 immediately Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Dial 911 All HF exposures should be considered an emergency Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended HF waste should be segregated from all incompatible chemicals such as caustics Hydrofluoric Acid HF Date 2 1 2013 PURDUE UNIVERSITY Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the HF SDS must be made ava
40. at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Seek medical attention immediately In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention and continue eye rinse during transport to hospital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek medical attention immediately Section 8 Special Handling and Storage Requirements e Avoid contact with skin eyes and clothing Avoid inhalation and ingestion Avoid formation and accumulation of dust Use only in a well ventilated area Keep away from heat shock friction and sources of ignition No smoking Prevent build up of electrostatic charge Use spark proof tools and explosion proof equipment Handle and open container with care Never work with flammable solid materials alone Flammable materials should be stored in a separate safety storage cabinet or room Flammable Solids Date 11 13 2013 PURDUE UNIVERSITY e Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances Any expired or unnecessary reactive materials should be properly disposed of as hazardous waste All flammable solids should be clearly label
41. combustible materials Take any precaution to avoid mixing with combustibles Store away from combustibles Oxidizers can have other associated hazards such as corrosive or toxic e g Nitric acid Sodium nitrite Make sure that all of the potential hazards are understood before handling any chemical Section 5 Personal Protective Equipment PPE Respirator Protection If oxidizing liquids or solids are being used outside of a chemical fume hood respiratory protection may be required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Respirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL As PPE in the event of a chemical spill clean up process Oxidizers Date 9 5 2013 PURDUE UNIVERSITY Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avo
42. condition and compatible with the material e Transport all corrosives in secondary containment such as polyethylene or other non reactive acid solvent bottle carrier Corrosives must be segregated from incompatible materials Incompatibilities will be noted in Section 10 of the SDS Stability and Reactivity Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal
43. conducted to minimize the volume of hazardous chemicals stored in the laboratory Always follow the chemical manufacturer s storage instructions if provided Flammable Liquids Storage Flammable liquids include any liquid with a flash point no greater than 93 C 200 F The following guidelines for storing flammable liquids must be followed in all laboratories Flammable and combustible liquids should be stored in flammable storage cabinets as shown in Figure 5 1 whenever possible No more than 10 gallons of flammable liquid is permitted to be stored outside of a flammable storage cabinet unless it is stored in a flammable safety can equipped with a spring loaded lid and an internal screen as shown in Figure 5 2 Domestic refrigerators or freezers must never be used to store flammable liquids Flammable liquids can only be stored in refrigerators or freezers that are designed for flammable materials most refrigerators are not intended for flammable storage Flammable liquids must be stored in well ventilated areas free from ignition sources Some organic solvents e g diethyl ether have a shelf life and can form organic peroxides over time while in storage These peroxide formers must be dated when received from the chemical manufacturer and disposed of once expired If any time sensitive chemicals are found to be past the manufacturer s expiration date they must be submitted to REM for hazardous waste disposal immediately
44. conducting any work with alkali metals designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Alkali Metals Date 9 26 2013 PURDUE UNIVERSITY Name Signature Date Stacey Opperwall 10 15 2014 Alkali Metals Date 9 26 2013 PURDUE UNIVERSITY Standard Operating Procedure Base Baths Alcoholic Hydroxide Solutions This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information
45. defined tasks The Laboratory Supervisor is responsible for ensuring that hazard assessments are performed and the certification s is written signed dated and readily available or posted in each location The Laboratory Supervisor is also responsible for ensuring that all lab personnel receive documented training on applicable hazard assessments The certification of hazard assessment should be reviewed at least annually and updated any time a process is modified or when a new task which presents a hazard is introduced into the lab 60 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy Hazard assessments can be organized using three formats by individual task e g pipetting hazardous liquids by location e g Chemistry Laboratory Room 1250 or by job title e g Chemistry Lab Technician Any of these formats is acceptable and often will be used in conjunction with each other to provide the safest laboratory work environment possible for employees The following subsections describe each hazard assessment format in more detail 6 3 1 Task Evaluation Hazard Assessment Task evaluation hazard assessments should be conducted for specific tasks such as preparing dilute hydrochloric acid solutions or an ozonolysis reaction and workup These types of hazard assessments should be written in a very detailed manner The following describes the steps that should be taken to perform a task evalu
46. details the minimum PPE requirements for all laboratories using hazardous chemicals These requirements do not apply to labs that involve solely mechanical computer laser or other non ionizing radiation or electrical operations The requirements listed do not cover all operations in all laboratories Some operations and procedures may warrant further PPE as indicated by the SDS the SOP for the chemical s being used facility policies or regulatory requirements Figure 6 1 illustrates the minimum PPE required when using hazardous chemicals in a laboratory 62 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy Safety Glasses or Goggles Lab Coat Chemical Resistant Gloves Long Pants Closed Toe Shoes Figure 6 1 Appropriate PPE for the Laboratory 6 4 1 Head Protection If there is a serious risk of chemical splash to the head a chemical resistant hoodie must be worn Each affected employee must wear protective helmets when working in areas where there is a potential for injury to the head from falling objects or bump hazards 6 4 2 Hearing Protection Hearing protection is not typically required in laboratory settings However if the lab seems excessively noisy e g operating equipment that is loud air handling unit is loud and it is difficult to communicate with co workers while in the lab contact REM 765 49 46371 for a noise level evaluation 63 The
47. employee exposure to hazardous chemicals The hierarchy of controls prioritizes hazard mitigation strategies on the premise that the best way to control a hazard is to systematically eliminate it from the workplace or substitute a less hazardous technique process or material If elimination or substitution are not feasible options administrative controls engineering controls and PPE must be used to provide the necessary protection The laboratory employee s responsibility is to follow administrative controls use engineering controls and wear PPE correctly and effectively 4 1 Routes of Exposure There are four primary routes of exposure in which hazardous substances can enter the body inhalation absorption ingestion and injection Of these the most likely routes of exposure in the laboratory are by inhalation and or skin absorption Many hazardous chemicals may affect people through more than one of these exposure modes so it is critical that protective measures are in place for each of these exposure routes 4 2 Engineering Controls and Safety Equipment Exposure to hazardous materials must be controlled to the greatest extent feasible by use of engineering controls Engineering controls to reduce or eliminate exposures to hazardous chemicals include e Substitution with less hazardous equipment chemicals or processes e g safety cans for glass bottles e Isolation of the operator or the process e g use of a glove box when
48. example of a bottle carrier is illustrated in Figure 5 10 Laboratory carts used to transport chemicals from one area to another shall be stable and in good condition Transport only a quantity which can be handled easily Plan the route ahead of time so as to avoid all steps or stairs Freight elevators not passenger elevators should be used to transport hazardous chemicals whenever possible The individual transporting the hazardous chemicals should operate the elevator alone if possible Avoid getting on an elevator when a person is transporting hazardous chemicals 54 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 15 Laboratory Security All laboratory personnel have a responsibility to protect university property from misuse and theft of hazardous materials particularly those that could threaten human health At a minimum the following security measures should be employed in all campus laboratories e The laboratory door should remain locked when not occupied e Always feel free to question anyone that enters the lab that you do not know and ask to see identification if necessary e If you see anything suspicious or someone displays suspicious behavior immediately report it to the Purdue Police Department by dialing 911 emergency or 765 494 8221 non emergency e Any sensitive information or particularly hazardous chemicals should not be stored out in the open w
49. flaw present Don t keep cracked glassware If the bottom of a graduated cylinder is chipped or broken properly dispose of it Always wear appropriate PPE when working with glassware and varying temperatures Always wear safety glasses 5 12 6 High Pressure Systems Working with high pressure systems in a laboratory can result in over pressurization explosion and the possible hazards of flying glass chemical exposure and fire All high pressure systems must be set up and operated with careful consideration of potential risks The following procedures should be followed when working with high pressure systems in the laboratory High pressure operations should be performed only in pressure vessels appropriately selected for the operation properly labeled and installed and protected by pressure relief control devices Vessels connecting hoses and any apparatus must be strong enough to withstand the stresses encountered at the intended operating temperatures and pressures and must not corrode or otherwise react when in contact with the materials it contains All pressure equipment should be visually inspected before each use 48 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 12 7 Vacuum Systems Vacuum work can result in an implosion and the possible hazards of flying glass splattering chemicals and fire All vacuum operations must be set up and operate
50. flow dynamics are achieved Most chemical fume hoods are not intended to be used with the sash fully open The sash should only be fully opened to add or remove equipment from the chemical fume hood Figure 4 1 Chemical Fume Hood Sash Approved Working Height Chemical fume hoods must be equipped with a continuous reading monitoring device to indicate adequacy of flow All lab employees must know how to read and interpret this gauge and check that the chemical fume hood is operating properly before using hazardous chemicals in the fume hood There are many different types of chemical fume hoods on campus so it is important that the lab employee understands the specific functions of each chemical fume hood used 20 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls Only apparatus and chemicals essential to the specific procedure or process should be placed in the chemical fume hood Extraneous materials from previous experiments should be removed and stored in a safe location outside the chemical fume hood Chemical fume hoods used for experimental work should not be used for chemical or material storage Chemical fume hoods used for chemical storage should be dedicated to chemical storage No experimental work should be conducted in storage chemical fume hoods All chemical containers used in chemical fume hoods including secondary containers e g beakers flasks reaction vesse
51. for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes Seek immediate medical attention and continue eye rinse during transport to hospital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Consult a physician Benzene Date 8 19 2013 PURDUE UNIVERSITY Section 8 Special Handling and Storage Requirements A designated storage area must be established for Benzene and the area should be posted with a Caution Carcinogen Reproductive Toxins or Extremely Toxic Chemicals label provided by REM as shown to the right Avoid contact with skin and eyes and inhalation Keep away from sources of ignition Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such as oxidizers e A suitable storage location is a flammable storage cabinet that does not contain incompatibles Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chem
52. handling air or water sensitive chemicals and e Use of forced ventilation systems e g chemical fume hood biological safety cabinet 4 2 1 Chemical Fume Hoods A chemical fume hood is a type of local ventilation installation that is designed to limit exposure to hazardous or toxic fumes vapors or dusts To determine if a chemical is required to be used inside of a chemical fume hood first check the SDS for that chemical Statements found in Section 2 on a SDS such as do not breathe dust fumes or vapors or 19 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls toxic by inhalation indicate the need for ventilation As a best practice always use a chemical fume hood for all work involving the handling of open chemicals e g preparing solutions whenever possible If a chemical fume hood is required or recommended to be used the following guidelines must be followed at all times Chemical fume hoods must be marked to indicate the proper sash position for optimum hood performance as illustrated in Figure 4 1 The chemical fume hood sash should be positioned at this height whenever working with hazardous chemicals that could generate toxic aerosols gases or vapors In general the sash height should be set at a level where the operator is shielded to some degree from any splashes explosions or other violent reactions which could occur and where optimum air
53. information in mind when disposing of empty containers e Triple rinse empty containers with a solvent capable of removing the original material e Collect the rinsate for disposal through REM e Identify triple rinsed dry odorless and empty containers by placing a Safe for Disposal label on the container Figure 7 5 Contact REM at 765 49 40121 to request a supply of these labels e Remove any cap that may cause the container to become pressurized when compacting e Arrange removal of these containers with the Building Services staff in your area or take these containers to the designated area beside the dumpster outside your building Figure 7 5 Safe for Disposal Label e f unable to remove residual hazardous materials from containers submit these to REM for pickup using the Hazardous Materials Pickup Request Form 73 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 5 Waste Disposal Procedures REM provides pickup services for all chemical waste generated on the West Lafayette campus A Hazardous Materials Pickup Request Form must be completed and submitted by the generator of the waste to initiate pickup services Once the pickup request has been processed REM staff will come to your lab to pick up the waste Average turnaround time is 3 5 days The following procedures must be followed in order to have hazardous waste removed from campus locatio
54. lab personnel the Purdue University Fire Department must still be contacted immediately by dialing 911 Table 4 1 Fire Classifications System Classification Fire Type Class A Ordinary fire wood and paper Class B Flammable liquids and gases Class C Electric fire Class D Combustible metal fire Class K Kitchen fire 4 2 7 Fire Doors Many laboratories may contain fire doors as part of the building design These doors are an important element of the fire containment system and should remain closed unless they are on a magnetic self closure or other automated self closing system Never disable an automatic door closure device e g placing a block under the door If you are unsure of whether a door is fire rated or not contact REM at 765 49 46371 and a staff member will come to the area to evaluate the specific door in question 25 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls 4 3 Administrative Controls Administrative controls are procedural measures which can be taken to reduce or eliminate hazards associated with the use of hazardous materials Administrative controls include the following e Ensuring that employees are provided adequate documented training for safe work with hazardous materials e Careful planning of experiments and procedures with zu MEER EADEATEON se TO DIRECT OR SCATTERED safety in mind Planning includ
55. more information contact REM at 765 49 6371 or visit the REM Training webpage http www purdue edu rem home files training htm 9 1 CHP Training As discussed in Chapter 1 of the CHP all laboratory employees Pls graduate students lab technicians managers post docs visiting scientists etc must receive documented CHP training before any work with hazardous materials occurs The Laboratory Supervisor is responsible for providing CHP training Initial CHP training should include the following e Review the lab specific CHP in its entirety e Review lab specific hazard assessments e Review lab specific SOPs e Review any other lab specific protocol or requirements Refer to Appendix A for the CHP Awareness Certification Form which can be used to document reading the CHP 9 1 1 Annual CHP Refresher Requirements After receiving the initial documented CHP training all lab employees must receive annual CHP refresher training as well This annual refresher training can be a condensed version of the initial CHP training but should include at least the following elements e Review of the lab specific hazard assessments review of PPE requirements e Review of the lab specific SOPs e Review of any additional lab specific rules and requirements e Review of chemical spill and lab emergency procedures 81 The official version of this document will only be maintained online Chapter 9 Training 9 2 PPE Training As discussed in
56. never be reused 6 4 6 Body Protection Each affected employee must wear protective clothing to protect the body from recognized hazards All unprotected skin surfaces that are at risk of injury should be covered Full length pants or full length skirt must be worn at all times by all individuals that are occupying the laboratory area shorts are not permitted Lab coats coveralls aprons or protective suits are required to be worn while working on or adjacent to all procedures using hazardous chemicals e g chemical bottle is open and the chemical is being poured transferred pipetted etc Laboratory coats must be appropriately sized for the individual and be fastened snap buttons are recommended to their full length Laboratory coat sleeves must be of a sufficient length to 65 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy prevent skin exposure while wearing gloves Flame resistant laboratory coats must be worn when working with pyrophoric materials or flammable liquids greater than 1 liter in volume It is recommended that 100 cotton or other non synthetic material clothing be worn during these procedures to minimize injury in the case of a fire emergency Laboratory coats should not be worn outside of a laboratory unless the individual is traveling directly to an adjacent laboratory work area Laboratory coats should not be worn in common areas such as break rooms
57. of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Lithium alkyls Date 2 1 2013 PURDUE UNIVERSITY Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended Wearing two pairs of nitrile gloves is recommended NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Lithium alkyls Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Face shield is also recommended Skin and Body Protection Flame resistant lab coats must be
58. organ toxicity single exposure respiratory system and central nervous system Specific target organ toxicity repeated exposure inhalation and central nervous system Molecular Formula CH Cl Form physical state Liquid Methylene Chloride Date 9 26 2013 PURDUE UNIVERSITY Color Colorless Boiling Point 40 C Flash Point NA not flammable Density 1 325 g L at 25 C Relative Vapor Density 2 93 Air 1 0 Section 4 Potential Hazards Methylene chloride is an OSHA regulated carcinogen It is an acutely toxic liquid that is extremely harmful if inhaled or absorbed through the skin Methylene chloride is toxic to the liver pancreas blood central nervous system heart and kidneys Exposure Limits OSHA PEL 8 HR TWA OSHA Short Term Exposure Limit ACGIH TLV TWA Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intend
59. other similar devices to immerse and remove objects from cryogenic liquids never immerse any part of your body into a cryogenic liquid 5 10 Nanoparticle Safety The American Society of Testing and Materials ASTM Committee on Nanotechnology has defined a nanoparticle as a particle with lengths in two or three dimensions between 1 and 100 nanometers nm Nanoparticles can be composed of many different base materials and may be of different shapes including nanotubes nanowires and crystalline structures such as fullerenes and quantum dots Nanoparticles present a unique challenge from an occupational health perspective as there is a limited amount of toxicological data currently available for review However some studies have shown that existing exposure control technologies have been effective in reducing exposure to nanoparticles Refer to the REM webpage for detailed procedures and guidance regarding the safe handling of nanoparticle http www purdue edu rem home booklets nanopolicy pdf 5 11 Sharps Handling Safety Sharps are defined as items capable of puncturing cutting or abrading the skin such as glass or plastic pipettes broken glass test tubes petri dishes razor blades needles and syringes with needles Sharps are often contaminated with hazardous chemicals and or infectious agents so multiple hazards are often encountered Employees that routinely work with sharps must be aware of the risk of being punctured or lacerated
60. prevent Long sleeves or lab coat Do not store in a cold room or other controlled environment without air Ms Long pants lever restrict pressure relief device Fully closed toe shoes supply Liquid nitrogen containers should be stored out of direct sunlight Figure 8 Liquid Nitrogen Label Section 9 Spill and Accident Procedures In the event of a large liquid nitrogen spill or release immediately evacuate the area and ensure others are aware of the spill Remember that frostbite and asphyxiation are the primary hazards so ensure people are protected from these hazards If there is an imminent threat pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Section 7 First Aid Procedures If inhaled Over exposure of liquid nitrogen may cause rapid suffocation due to displacement of oxygen With asphyxiation unconsciousness may happen without warning If person becomes dizzy move them to a well ventilated area and seek immediate medical attention dial 911 In case of skin contact Skin contact with liquid nitrogen may cause severe cold burns and frostbite Flesh freezes very rapidly and may be torn when attempting to be withdrawn from object If frostbite or freezing occurs the following
61. steps should be taken 1 Flush the area thoroughly with tepid water Do not apply heat or rub the affected area 2 Protect the area with bulky dry and sterile dressings 3 Seek immediate medical attention dial 911 Liquid Nitrogen Date 5 9 2014 PURDUE UNIVERSITY In case of eye contact Eye exposure to liquid nitrogen can cause permanent and irreversible damage Delicate eye tissue can be damaged by exposure to the cold gas alone If liquid nitrogen is splashed into the eyes the following steps should be taken flush the eyes with water for 15 minutes and seek immediate medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures There is typically no waste generation involved with the use of liquid nitrogen However if waste disposal questions arise please contact the REM Hazardous Materials Management Section at 49 40121 Section 12 Safety Data Sheet SDS A current copy of the SDS for liquid nitrogen must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldr
62. such as polyethylene or other non reactive acid solvent bottle carrier Flammable combustible liquids must be segregated from incompatible materials such as oxidizers e g Hydrogen peroxide Nitric acid Incompatibilities will be noted in Section 10 of the SDS Stability and Reactivity If flammable liquids will be stored in refrigerators or freezers these will be specially modified or purpose made flammable safe refrigerators and freezers which have no internal sources of ignition posed by an internal light or thermostat circuit Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After H
63. that must be done properly to avoid accidents and potential injuries The following subsections discuss how to properly ship chemical off campus using a shipping company how to transport chemicals on campus using a Purdue owned vehicle and how to safely move chemicals by foot across campus 51 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 14 1 Shipping Hazardous Chemicals off Campus Shipping chemicals research samples or other similar materials off campus is potentially regulated by the Department of Transportation DOT and or other regulatory agencies Chemicals regulated for shipping require very specific types of packaging labeling and documentation and must be prepared by trained personnel REM makes the determination on whether a chemical is classified as hazardous for transportation purposes Unless the researcher is DOT trained they are not authorized to make this determination Shipments that are not prepared by trained personnel can result in delays loss of research samples and potential regulatory fines REM can provide assistance by either providing shipment services or if necessary training personnel on the proper shipping procedures More information about shipping chemicals can be found on the REM webpage http www purdue edu rem hmm shiphm htm As previously stated REM prepares all regulated chemicals for shipment according to DOT requirements H
64. the form of standard operating procedures SOPs hazard assessments and any other written lab specific operating procedures that address the hazards and how to mitigate risks The following instructions detail how this CHP template should be used and customized by each laboratory e Review this template CHP provided by REM e Insert your lab specific standard operating procedures SOPs into your customized CHP under Tab 1 located in the back of the CHP document More details regarding SOPs can be found in Chapter 4 of the CHP e Insert all other documented lab specific rules requirements and procedures e g equipment protocols internal lab inspections etc under Tab 2 e Insert your lab specific hazard assessments under Tab 3 More details regarding hazard assessments can be found in Chapter 6 of the CHP e Review update if necessary and retrain all employees on the lab specific CHP at least annually 1 4 Employee Rights and Responsibilities As part of the OSHA Laboratory Standard employees and other personnel who work in laboratories have the right to be informed about the potential hazards of the chemicals in their 3 The official version of this document will only be maintained online Chapter 1 Introduction work areas and to be properly trained to work safely with these substances This includes custodial and maintenance personnel support staff who work to maintain laboratories All personnel including principal in
65. three of these products expire and must be maintained annual purchases must be made to keep the kit current If inhaled Dial 911 Move to fresh air If the person is not breathing give artificial respiration Avoid mouth to mouth contact In case of skin contact Dial 911 Immediately within seconds flush affected area for at least 5 minutes Remove all contaminated clothing Wearing compatible gloves massage calcium gluconate 2 5 gel into the affected area Re apply every 15 minutes until medical help arrives NOTE Hydrofluoric acid exposure is often treated with calcium gluconate a source of Ca that sequesters the fluoride ions HF chemical burns can be treated with water and 2 5 calcium gluconate gel or special rinsing solutions However because it is absorbed medical treatment is necessary Intra arterial infusions of calcium chloride have also shown great effectiveness in treating HF burns In some cases amputation has been required In case of eye contact Dial 911 Flush eyes with water for 5 minutes Then use 1 Calcium Gluconate emergency eyewash solution NOTE Do not open the emergency eyewash solution seal unless it needs to be used Use the entire 120 ml content during an emergency eye exposure Emergency eyewash solution is for single use only If swallowed Dial 911 Do not induce vomiting Drink large quantities of water then drink 12 ounces of milk of magnesia Never give anything by mouth to uncon
66. to an atmospheric contaminant in the absence of PEL As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended Check the resources below for the most suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific oxidizing liquids or solids being used Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required A face shield may also be appropriate depending on the specific application Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are o
67. until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 7726 95 6 GHS Classification Toxic Corrosive Molecular Formula Br Form physical state Fuming Liquid Color Red Brown Boiling Point 58 8 C 137 8 F Bromine Date 2 17 2014 PURDUE UNIVERSITY Uses Bromine is mainly used in brominated flame retardants Bromine compounds can also be used for water treatments as a gasoline additive in pesticides in photographic chemicals and in the manufacture of pharmaceuticals Section 4 Potential Hazards Bromine
68. warm water and soap NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with HF Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety goggles and face shield are required Skin and Body Protection Lab coat amp natural rubber apron full length pants and closed toe rubber or leather shoes are required NOTE If HF is used in a cleanroom the cleanroom gown will substitute for lab coat and full length pants In addition all the other PPE listed in this section is applicable Hygiene Measures Avoid contact with skin eyes and clothing Wash hands before breaks and immediately after handling the product Hydrofluoric Acid HF Date 2 1 2013 PURDUE UNIVERSITY Section 6 Engineering Controls HF can only be used in a properly functioning chemical fume hood or glove box The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures First Aid Kit All labs that store and use HF must have a HF First Aid Kit with the following supplies e Calcium Gluconate 2 5 Gel e Milk of Magnesia e Eyewash 1 Calcium Gluconate Solution NOTE All
69. 15 2014 Pyrophoric Materials Date 9 18 2013 PURDUE UNIVERSITY Standard Operating Procedure Sodium Azide This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone 765 494 4573 Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 26628 22 8 GHS Classification Potentially explosive compound PEC water reactive acute toxicant Molecular Formula NaN Form physical state Solid Color White Sodium Azide Date 3 27 2014 PURDUE UNIVERSITY Section 4 Potential Hazards May be harmful if inhaled May cause respiratory tract irritation May be fatal if ab
70. 4 of the CHP details the storage requirements for reactive chemicals B 1 6 Oxidizers An oxidizing solid liquid is a solid liquid which while in itself is not necessarily combustible may generally by yielding oxygen cause or contribute to the combustion of other material Hydrogen peroxide nitric acid and nitrate solutions are examples of oxidizing liquids commonly found in a laboratory Sodium nitrate Sodium perchlorate and Potassium permanganate are examples of oxidizing solids commonly found in a laboratory Chapter 5 7 7 of the CHP details oxidizer storage requirements B 1 7 Organic Peroxides An organic peroxide is an organic liquid or solid which contains the bivalent 0 0 structure and may be considered a derivative of hydrogen peroxide where one or both of the hydrogen atoms have been replaced by organic radicals The term also includes organic peroxide formulations mixtures Such substances and mixtures may e Be liable to explosive decomposition e Burn rapidly e Be sensitive to impact or friction or e React dangerously with other substances Chapter 5 7 7 of the CHP details organic peroxide storage requirements 89 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions B 1 8 Explosives An explosive substance or mixture is a solid or liquid substance or mixture of substances which is in itself capable by chemical reaction of producing gas at suc
71. 765 494 4573 Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP Building Room Number Section 2 Type of SOP C Process Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A a mas 0 125in dia sa Pr E EN GHS Classification Substances which in contact with water emit flammable N Lote uztPet SB mylar under argon gases causes serious skin corrosion and eye damage Molecular Formula N A Form physical state solid Color N A Alkali Metals Date 9 26 2013 PURDUE UNIVERSITY Boiling point N A Section 4 Potential Hazards Alkali metals are extremely reactive with water producing flammable hydrogen gas that can ignite spontaneously It also produces caustic hydroxide solution i e Sodium hydroxide upon contact with water It may be harmful if inhaled ingested or absorbed through the skin Alkali metals are extremely destructive to the tissue of the mucous membranes and upper respiratory tract Alkali metals include Lithium Sodium Potassium Rubidium Cesium and Francium ae et Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering an
72. IVERSITY e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Nitrile gloves are recommended and have been shown to provide excellent protection against potassium cyanide The user should wear two pairs of gloves as a precautionary measure Use proper glove removal technique to avoid any skin contact Never reuse disposable gloves Check the resources below for a more suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with potassium cyanide Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Use only in a chemical fume hood where the sash can be positioned to protect the user s face and eyes If there is an elevated risk of splash hazard a face shield and chemical splash goggles must be used Skin and Body Protection Lab coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent
73. If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Section 8 Special Handling and Storage Requirements A designated storage area must be established for flammable and combustible liquids such as a flammable storage cabinet as shown to the right No more than 37 liters 10 gallons of flammable liquid is permitted to be stored outside of a flammable storage cabinet area Do not over purchase only purchase what can be safely stored in the laboratory Avoid contact with skin eyes and inhalation Keep away from sources of ignition Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such as oxidizers More handling details are described below in Section 13 Follow laboratory sup
74. It is important for these employees to take precautions and properly handle sharps in order to prevent injury and potential disease transmission These employees should use appropriate PPE e g puncture resistant gloves tools barrier protection sharps waste containers and engineering controls to protect themselves Refer to the REM webpage for detailed procedures regarding sharps handling and disposal procedures https www purdue edu rem home booklets sharps pdf 44 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 12 Equipment Apparatus and Instrument Safety 5 12 1 Centrifuges The following safety guidelines should be followed when operating centrifuges Before centrifugation e Centrifuges must be properly installed and operated only by trained personnel Centrifuges cannot be placed in the hallway of a building they must remain inside of the laboratory e Train each operator on proper operating procedures review the user manual e Use only rotors compatible with the centrifuge Check the expiration date for ultracentrifuge rotors e Check tubes bottles and rotors for cracks and deformities before each use e Make sure that the rotor tubes and spindle are dry and clean e Examine O rings and replace if worn cracked or missing e Never overfill centrifuge tubes don t exceed full e Always cap tubes before centrifugation e Always balance bu
75. OTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with methyltrichlorosilane designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Methyltrichlorosilane Date 12 13 2013 PURDUE UNIVERSITY Standard Operating Procedure Nitric Acid This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab
76. PURDUE UNIVERSITY CHEMICAL HYGIENE PLAN AND HAZARDOUS MATERIALS SAFETY MANUAL Adopted February 2014 Updated July 2 2014 The official version of this document will only be maintained online PURDUE UNIVERSITY Chemical Hygiene Plan and Hazardous Materials Safety Manual Laboratory Specific Plan This is the Chemical Hygiene Plan specific to the following areas Building s Brown Chemistry Room Number s 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Principal Investigator Supervisor Tong Ren Department Chemistry Revised Must be reviewed at least annually 09 26 2014 Important Telephone Numbers 1 911 for All Emergencies 2 765 49 48221 Purdue Police Department Non Emergency Line 3 765 49 46919 Purdue Fire Department Non Emergency Line 4 765 49 46371 Purdue REM Do Not Use for an Emergency All laboratory chemical use areas must maintain a work area specific Chemical Hygiene Plan which conforms to the requirements of the OSHA Occupational Exposure to Hazardous Chemicals in Laboratories 29 CFR 1910 1450 Purdue University laboratories may use this document as a starting point for creating their work area specific Chemical Hygiene Plan Minimally this cover page is to be edited for work area specificity non West Lafayette laboratories are to place their own emergency fire and police telephone numbers in the space above and the Purdue Chemical Hygiene Plan Awareness Cer
77. Peroxide Self Reactive Oxidizer Environmental Toxicity Acute Toxicity Severe Figure 2 2 GHS Hazard Pictograms GHS labeling requirements are only applicable to chemical manufacturers distributors and shippers of chemicals GHS labeling requirements are not required for chemicals being stored in a laboratory However since most chemicals stored in the laboratory have been purchased from a chemical manufacturer the GHS labeling and pictogram requirements are very relevant and must be understood by laboratory employees Figure 2 3 illustrates the GHS label format showing the required elements 11 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems ACETONE PRODUCT IDENTIFIER HAZARD PICTOGRAMS Code Product Name SUPPLIER IDENTIFICATION Company Name Street Address City State SIGNAL WORD Postal Code Danger Phone Number HAZARD STATEMENT PRECAUTIONARY STATEMENTS Keep away from heat sparks open flames hot surfaces No smoking Avoid breathing dust fumes gas mist vapors and spray IF IN EYES Rinse cautiously with water for several minutes Remove contact lenses if present and easy to do Continue rinsing Repeated exposure may cause skin dryness and cracking In Case of Fire Use water spray alcohol resistant foam dry chemical or carbon dioxide First Aid Move out of dangerous area Consul
78. RSITY Store Waste Store hazardous waste in closed containers and in a designated area Hydrogen peroxide waste should be segregated from all incompatible chemicals such as organic compounds Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the hydrogen peroxide SDS must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with hydrogen peroxide designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The P
79. SOP Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 7727 37 9 GHS Classification Gas under pressure cryogenic liquid Molecular Formula N Form physical state Liquid Boiling Point 196 C 320 F Liquid Nitrogen Date 5 9 2014 PURDUE UNIVERSITY Relative Vapor Density 0 97 air 1 Section 4 Potential Hazards Liquid nitrogen is a cryogenic liquid that may cause severe frostbite or eye damage upon contact Extremely cold liquid and gas under pressure Expands by a factor of 700 upon vaporization Can cause rapid suffocation due to displacement of oxygen Avoid breathing gas Substances may become brittle upon contact and shatter May cause an explosion of a sealed container Symptoms to exposure included frostbite dizziness salivation nausea vomiting or loss of mobility and or consciousness Section 5 Personal Protective Equipment PPE For quantities less than 100 mL safety glasses lab gloves long pants and close toe shoes are adequate Figure 1 For intermediate quantities 100 mL 1 L also use splash goggles face shield and cryogenic gloves Figure 2 For quantities greater than 1 L or filling a secondary Dewar a cryogenic apron should be used in addition to the general use intermediate requirements Figure 3 Figure 1 Small
80. Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Seek medical attention immediately Formaldehyde Date 6 18 2013 PURDUE UNIVERSITY In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Seek medical attention immediately In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention and continue eye rinse during transport to hospital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek medical attention immediately Section 8 Special Handling and Storage Requirements e A designated storage area must be established for formaldehyde and the area should be posted with a Caution Carcinogen Reproductive Toxins or Extremely Toxic Chemicals label provided by REM as shown to the right Avoid contact with skin and eyes and inhalation Keep away from sources of ignition Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such as oxidizers e A suitable storage location is a flammable stora
81. Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone 765 494 4573 Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP Building Room Number Section 2 Type of SOP C Process XX Hazardous Chemical C Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CASH 7697 37 2 GHS Classification Target Organ Effect Corrosive Oxidizer Molecular Formula HNO3 Form physical state Liquid Color Colorless light yellow vi Uy Mallinckrodt a HEMIC ALS Nitric Acid Date 7 2 2013 PURDUE UNIVERSITY Boiling point 83 9 100 C Uses Nitric acid is commonly found in the laboratory and there are several uses A few common uses include nitration purification of metals cleaning agents Aqua Regia and the production of nylon precursors and agricultural fertilizers Section 4 Potential Hazards Nitric acid is an oxidizer that may intensify fires Fire conditions may cause the formation of hazardous nitrogen oxides Can react violently with organic chemicals such as organic solvents Nitric acid may be harmful if inhales ingested or absorbed through the
82. Taking a routine chemical inventory can reduce the number of unknown chemicals and the tendency to stockpile chemicals REM recommends that all laboratories take a chemical inventory at least annually Depending on the type of chemicals being used and stored in a laboratory REM may require that a chemical inventory be prepared for a room work unit or department e g Department of Homeland Security Chemical Facility Anti Terrorism Standards Inventory on a routine basis 5 4 Safety Data Sheets The SDS provides comprehensive information that is imperative for the safe handling of hazardous chemicals Carefully read the label and SDS and make sure that you understand the information provided in this document before using a chemical In some cases it may be necessary to do additional research The Laboratory Supervisor should be consulted if necessary It is important that all lab employees have access to SDS for all hazardous chemicals that are stored in the lab Access can mean storing hard copies of SDS in the lab or some other easily accessible location e g departmental main office or can mean storing electronically by a means that is also accessible to all lab personnel e g shared network drive To obtain a copy of a SDS contact the chemical manufacturer or REM at 765 49 46371 Many manufacturers SDS can be found online at REM s SDS webpage or other websites such as Siri MSDS Index The links to these resources are included below
83. Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical Section 3 Physical Chemical Properties Physical Chemical Properties CAS 151 50 8 CI Hazardous Class GHS Classification Acute toxicity oral inhalation dermal Specific target organ toxicity single and repeated exposure Corrosive to metals Acute aquatic toxicity Molecular Formula KCN Form physical state Solid Color White Potassium Cyanide Date 11 26 2013 PURDUE UNIVERSITY pH 11 5 at 20 g L at 20 C Density 1 520 g cm Water solubility 400 g L at 20 C soluble Section 4 Potential Hazards Potassium cyanide is an acute and chronic toxin It is extremely harmful possibly fatal if inhaled ingested or in contact with the skin or eyes Causes damage to organs through prolonged or repeated exposure It presents toxicity by skin absorption through open wounds and by inhalation or dust This substance inhibits cellular respiration which leads to anoxia May be corrosive to metals Potassium cyanide exposed to acid liberates hydrogen cyanide which is an acutely toxic and deadly gas Exposure Symptoms If swallowed can cause tissue anoxia characterized by weakness headache dizziness confusion cyanosis bluish skin due to deficient oxygena
84. UV light check to ensure all ethidium bromide has been removed The decontamination solution should be collected in an appropriate container and labeled as hazardous waste i e Water 95 Hypophosphorus Acid 3 Sodium Nitrite 1 Ethidium Bromide 1 Note Hypophosphorous acid is a DEA List chemical When purchasing this product you and your PI will have to fill out and sign an Authorized Purchaser form This is simply a company s method of ensuring you will not misuse the material Section 9 Spill and Accident Procedures Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Follow the decontamination procedures detailed above Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 No
85. VERSITY Standard Operating Procedure Hydrogen Peroxide This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 7722 84 1 GHS Classification Oxidizing Liquid Acute Toxicity Skin Corrosion Serious Eye Damage Acute Aquatic Toxicity Molecular Formula HO Form physical state Liquid Color Colorless Hydrogen Peroxide Date 7 12 2013 PURDUE UNIVERSITY Boiling point 108 C Uses Due to its strong oxidizing properties hydrogen peroxide has several applications It is
86. a number of programs that apply to laboratory safety a few of which include biological safety laser safety personal protective equipment program radiation safety development of standard operating procedures as well as the CHP REM also performs numerous safety inspections of facilities throughout the year to monitor compliance with regulatory requirements REM provides a variety of services such as training chemical biological and radioactive waste pickups and safety consultation More detailed information regarding all of REM s resources and services can be found on the REM website http www purdue edu rem 1 6 Integrated Safety Plan It is the policy of Purdue University to integrate environmental health and safety into all operations The Integrated Safety Plan ISP is Purdue University s safety program which is facilitated by REM and was developed to provide a framework for laboratories to comply with environmental health and safety EHS regulations The ISP assists in communication of EHS 7 The official version of this document will only be maintained online Chapter 1 Introduction issues across the organization and calls for departmental level safety committees and individual self audits The ISP provides indemnification from regulatory fines for units with a certified safety program An ISP certified safety program must have the following elements e Regular safety committee meetings e Means of communicating safet
87. abel more comprehensive hazard information can be found by referencing the SDS for that chemical The OSHA Laboratory Standard defines a hazardous chemical as any element chemical compound or mixture of elements and or compounds which is a physical or health hazard This definition of a hazardous chemical and the GHS primary classes of chemicals are briefly discussed below 3 1 Physical Hazards A chemical is a physical hazard if there is scientifically valid evidence that it is flammable combustible compressed gas explosive organic peroxide oxidizer pyrophoric self heating self reactive or water reactive Each physical hazard is briefly defined below Refer to Appendix B section B 1 for detailed information on each physical hazard e Explosives A liquid or solid which is in itself capable by chemical reaction of producing gas at such a temperature and pressure and at such a speed as to cause damage to the surroundings e Flammable Liquids Materials which under standard conditions can generate sufficient vapor to cause a fire in the presence of an ignition source and have a flash point no greater than 93 C 200 F e Flammable Solid A solid which is readily combustible or may cause or contribute to a fire through friction e Gases under Pressure Gases which are contained in a receptacle at a pressure not less than 280 kPA at 20 C or as a refrigerated liquid e Organic Peroxide A liquid or solid which contains the bivalent
88. act poison It should be handled with extreme care i e beyond what is generally required to handle other mineral acids Owing to its low dissociation constant HF as a neutral lipid soluble molecule penetrates tissue more rapidly than typical mineral acids Because of the ability of hydrofluoric acid to penetrate tissue poisoning can occur readily through exposure of skin or eyes or when inhaled or swallowed Symptoms of exposure to hydrofluoric acid may not be immediately evident HF interferes with nerve function meaning that burns may not initially be painful Accidental exposures can go unnoticed delaying treatment and increasing the extent and seriousness of the injury HF is acalcium seeker A person cannot sense when it comes in contact with the skin But it dissolves the calcium in the bone HF burns are often not evident until a day later If not stored handled and disposed of properly HF can pose a serious threat to the health and safety of laboratory personnel emergency responders and waste handlers Hence it is important to thoroughly understand the properties of HF and follow all safety protocols to properly store and handle HF Potential Health Effects Inhalation Toxic if inhaled Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract Skin May be fatal if absorbed through skin Causes skin burns Eyes Causes eye burns Causes severe eye burns Ingestion May be fatal if swall
89. acturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Oxidizers Date 9 5 2013 PURDUE UNIVERSITY Standard Operating Procedure Piranha Solution This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone 765 494 4573 Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous C
90. after handling the product Section 6 Engineering Controls Use of piranha solution should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician Piranha Solution Date 3 14 2014 PURDUE UNIVERSITY In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Section 8 Special Handling and Storage Requirements Before using Piranha solution more stable methods of removing stains tars or clogs should be attempted Often glassware can be cleaned if left with a cleaning solution An immediate example for a suitable substitute prior to using Piranha is 98 Sulfuric
91. age segregation and labeling of chemicals and equipment safety must be established and known by all laboratory personnel This chapter details how laboratories should be managed at Purdue 5 1 Laboratory Safety Guidelines All laboratory employees must have a good understanding of the hazards associated with the chemicals being used and stored in the lab Basic factors such as the physical state gas liquid or solid of the chemical and the type of facilities and equipment involved with the procedure should be considered before any work with hazardous materials occurs 5 1 1 Laboratory Safety Questions Many factors are involved is laboratory safety Asking and answering the following questions will help address many of the factors that should be considered when it comes to laboratory safety e Is the material flammable explosive corrosive or reactive e ls the material toxic and if so how can be exposed to the material e g inhalation skin or eye contact accidental ingestion accidental puncture e What kind of ventilation do need to protect myself e What kind of PPE e g chemical resistant gloves respirator and goggles do need to protect myself e Will the process generate other toxic compounds or could it result in a fire explosion or other violent chemical reaction e What are the proper procedures for disposal of the chemicals e Dol have the proper training to handle the chemicals and carry out the proces
92. ained online Chapter 5 Laboratory Management Plan Table 5 1 Chemical Compatibility Chart Acid Acid Acid Base Base ER Toxic Toxic 3 Organic 5 er N 3 Oxidizer 3 2 Reactive inorganic organic oxidizer inorganic organic inorganic organic solvent Acid inorganic X X X X X Acid organic X X X X X X Acid oxidizer Base inorganic Base organic Oxidizer Toxic inorganic Toxic organic x x x x x x x x Xx Reactive x x x x Xx Xx x x Organic solvent x x XxX Xx 5 7 Chemical Storage Requirements Proper storage of chemicals is an essential component to a laboratory safety program Improper chemical storage practices can cause undesired chemical reactions which may form hazardous products that can lead to employee exposure or possibly fires and property damage All lab employees should carefully read each chemical s SDS and container label before deciding how to store a chemical as these will often indicate any special storage requirements that may be necessary The following subsections describe chemical storage requirements in more detail 5 7 1 General Chemical Storage The following general chemical storage guidelines must be followed in all laboratories Each chemical in the laboratory must be stored in a specific location and returned there after each use Accepta
93. al containers as well 5 6 Chemical Segregation All chemicals must be stored according to chemical compatibility Once segregated by chemical compatibility they can then be stored alphabetically Information regarding chemical compatibility can be found in the SDS primarily in Section 7 Handling and Storage and Section 10 Stability and Reactivity If unsure of proper segregation procedures contact the Laboratory Supervisor for assistance Chemical segregation can be achieved by either isolation e g organic solvents stored in a flammable cabinet physical distance e g acids and bases are stored on opposite sides of a chemical storage room or secondary containment e g placing oxidizing acids such as nitric acid into a secondary containment to segregate from organic acids such as formic acid as shown in Figure 5 7 In the most general terms proper segregation can be achieved by e Storing acids away from bases and toxics e Storing oxidizers away from organic chemicals and e Storing reactive and acutely toxic materials away from all other chemicals Table 5 1 illustrates a more detailed chemical compatibility logic that can be used for chemical storage Hazard classes marked by an X need to be segregated from each other e g Acid inorganic must be segregated from Base inorganic Contact REM at 765 49 40121 with questions regarding chemical segregation 33 The official version of this document will only be maint
94. alent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of pyrophoric material must be conducted in an inert atmosphere use of a glove box is recommended The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Seek medical attention immediately In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Seek medical attention immediately In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention and continue eye rinse during transport to hospital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek medical attention immediately Sect
95. alkali metals should be placed into secondary containment as a precautionary measure Suitable storage locations include inert gas filled desiccators or glove boxes flammable storage cabinets that do not contain aqueous or other incompatible chemicals or intrinsically safe refrigerators or freezers that also do not contain aqueous or other incompatible chemicals Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu r
96. als than others The SDS for the specific chemical being handled and the glove manufacturer s glove chart should be consulted to select the most appropriate glove Do not purchase gloves from a manufacturer that does not provide an adequate glove chart It is recommended that each lab purchase a general purpose disposable nitrile glove nitrile gloves are typically more versatile and provide resistance to a wider range of chemicals than latex gloves do with a minimum of a 4 mil thickness that is suitable for general chemical handling When handling chemicals with harmful temperature extremes such as liquid nitrogen or autoclaves appropriate protection such as cryogenic gloves or heat resistant gloves must be worn The volume of hazardous chemical being handled should be considered as well For example if working with a small volume of a sodium hydroxide solution disposable chemical resistant gloves provide adequate protection But if working with a large volume of sodium hydroxide as with a base bath for instance a more durable glove such as a butyl rubber should be selected to provide adequate protection _ Chemical resistant gloves must not be worn outside of the laboratory e g hallways elevators offices to avoid contamination of public areas Gloves should also be removed prior to handling any equipment that could likely result in cross contamination e g water fountains telephones computer work stations Disposable gloves must
97. ant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Neoprene nitrile and fluorinated rubber gloves are recommended Check the resources below for the most suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the chemical being used Refer to glove selection chart from the links below Bromine Date 2 17 2014 PURDUE UNIVERSITY http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required A face shield may also be appropriate depending on the specific application Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must
98. are used to convey health physical and environmental hazard information assigned to a GHS hazard class and category e Signal Words such as Danger for more severe hazards or Warning for less severe hazards are used to emphasize hazards and indicate the relative level of severity of the hazard assigned to a GHS hazard class and category e Hazard statements e g Danger Extremely Flammable Liquid and Vapor are standard phrases assigned to a hazard class and category that describe the nature of the hazard and e Precautionary statements are recommended measures that should be taken to minimize or prevent adverse effects resulting from exposure to the hazardous chemical GHS also standardizes the hazard pictograms that are to be used on all hazard labels and SDSs There are 9 pictograms that represent several defined hazards and include the harmonized hazard symbols which are intended to convey specific information about each hazard Figure 2 2 illustrates these GHS hazard pictograms 10 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Carcinogen Respiratory Sensitizer Reproductive Toxicity Target Organ Toxicity Mutagenicity Flammable Pyrophoric Self Heating Emits Flammable Gas Organic Peroxide Irritant Dermal Sensitizer Acute Toxicity harmful Narcotic Effects T Gas Under Pressure Corrosive Explosive Organic
99. area be covered with an appropriate liner i e plastic chemical resistant liner If the liner becomes contaminated it can be replaced and disposed of as ethidium bromide contaminated debris If a liner cannot be used then the surfaces of the work area should be decontaminated routinely All spills or evidence of contamination should be cleaned immediately Avoid contact with skin and eyes and inhalation Keep containers tightly closed Store in a cool dry and well ventilated area Containers which are opened must be carefully resealed and kept upright to prevent leakage Routinely decontaminate the area where ethidium bromide is used Decontamination procedures Ethidium Bromide Date 7 19 2013 PURDUE UNIVERSITY are detailed below Decontamination Procedures e Prepare the following decontamination solution o 4 2 grams of sodium nitrite NaNO o 20 milliliters of 50 hypophosphorous acid solution H3PO o 300 milliliters of water Soak a paper towel in the decontamination solution and thoroughly wash the contaminated area Once the contaminated area has been thoroughly washed with the decontamination solution rinse the area 5 times with tap water using a clean paper towel for each rinse Soak all spent paper towels in the decontamination solution for one hour Gently wring out excess solution and dispose of as hazardous waste with contaminated gloves pipette tips or any other solid ethidium bromide debris Using a
100. arm station to evacuate the building Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Dial 911 Potassium Cyanide Date 11 26 2013 PURDUE UNIVERSITY Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet or lab cabinet is recommended Never combine cyanide waste streams with acid waste streams as this will generate an acutely toxic gas Do not combine cyanide waste with other waste streams such as organic solvent waste the disposal cost is extremely high Keep cyanide waste separated from other wastes as much as possible Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange
101. ass contact e The outer containers should remain tightly secured during transport Measures should be taken to avoid movement of the outer containers For example the containers should be secured using a strap or an empty box can be used to fill the gap between the last box and the sidewall of the vehicle e The outer container must be labeled in a manner that identifies the contents e g corrosives flammables e Transport with two or more people if possible e Be prepared for unseen accidents At least one person should be knowledgeable of the materials being transported An inventory with an estimated volume or weight per hazard should be recorded and available during transport e g 5 gallons of flammable liquid and 10 pounds of toxic solids e Prepare a spill kit prior to transport Material such as appropriate PPE absorbent material and an empty bucket is sufficient for most small spills e Carry a cell phone and know who to call in the event of an emergency The Purdue Fire Department will respond to on campus emergencies Dial 911 from a Purdue phone or 765 49 48221 from a cell phone to contact Purdue Police dispatch 53 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 14 3 Transporting Chemicals on Campus via Foot Transporting small volumes of chemicals across campus via foot e g from two neighboring campus buildings is acceptable as long as it is done prope
102. aste Disposal Guidelines http www purdue edu rem home booklets hwdg pdf 69 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 2 Waste Identification and Labeling All chemical constituents in a hazardous waste container must be identified by knowledgeable laboratory personnel Not only is this required by the EPA it also ensures that waste can be properly characterized and disposed of by REM If there is uncertainty about the composition of a waste stream resulting from an experimental process laboratory employees must consult the laboratory supervisor for assistance In most cases careful documentation and review of all chemical products used in the experimental protocol will result in accurate waste stream characterization Additionally review SDSs specifically Section 2 Hazard Identification and Section 13 Disposal Considerations to obtain information about hazardous constituents and characteristics All waste should be properly labeled as soon the first drop of waste enters a waste container Containers must be labeled and clearly marked with words that describe the contents of the waste and the words Hazardous Waste Hazardous waste should be listed completely on the label provided by REM in a percentage format as shown in Figure 7 1 Listing accurate percentages is not as important 5 is acceptable and constituents less than 1 can be listed as trace
103. at can be safely stored in the laboratory e Containers should be labeled appropriately Label should indicate the name of the chemical s in the container Avoid using chemical abbreviations acceptable if a legend is present in the lab and formulae Glassware with excessive grime should first be rinsed with an appropriate solvent such as water and a little acetone Collect the rinsate in a separate container label with all constituents and submit to REM as waste If the glassware is greased excess grease should be removed with a paper towel Do not place broken glassware in the base bath as this may break the glass completely and produce glass shards Check all items before placing in the bath Do not leave glassware in the base bath for more than one overnight period Prolonged soaking in the bath will lead to degradation and consequent thinning of the glass Always use inside a chemical fume hood Take care not to cause the bath to overflow The base bath should be placed in a tray capable of containing the full bath volume in the event that the bath container fails Keep container upright amp closed in a dry and well ventilated place Base baths solutions must be stored in appropriate containers such as a heavy duty HDPE Nalgene container Do not store base bath solutions in metal containers Do not store base bath solutions Rubbermaid containers or other non chemical approved storage containers Avoid contact with skin and eyes Avoid inhalation
104. ation and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Diethyl Ether Date 8 19 2013 PURDUE UNIVERSITY Name Signature Date Stacey Opperwall 10 15 2014 Diethyl Ether Date 8 19 2013 PURDUE UNIVERSITY Standard Operating Procedure Ethidium Bromide This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone 765 494 4573
105. ation hazard assessment e Describe the task e List hazards associated with each body part e Determine PPE requirements for each hazard e List other control measures required such as engineering and administrative controls See the REM webpage for the task evaluation hazard assessment template http www purdue edu rem home files forms htm ppe 6 3 2 Location Evaluation Hazard Assessment Location evaluation hazard assessments should be conducted for specific areas laboratories These types of hazard assessments should be written in a comprehensive manner that includes the majority of hazards present in a specific location e g flammable and corrosive liquids This type of hazard assessment is the most commonly used in laboratories and should be posted in a location within the lab where it is easily accessed by personnel e g posted near the front door of the lab If employees perform specific tasks not covered by the laboratory hazard assessment then it will be necessary to perform another type of hazard assessment such as the task evaluation assessment that does address the specific hazards of that task The following describes the steps that should be taken to perform a task evaluation hazard assessment e Identify the hazards e List each task where hazard is present e Determine PPE requirements for each task e List other control measures required engineering and administrative controls 61 The official version of thi
106. ations or the West Lafayette wastewater treatment plant s requirements see section 7 7 of this chapter for information and guidance for acceptable sink disposal practices Hazardous wastes can be liquid solid gas or sludge They can be discarded chemicals or mixtures generated from research and teaching operations commercial products e g cleaning fluids or pesticides or by products of manufacturing processes All hazardous waste falls into one of the following categories e Characteristic Wastes includes wastes that are ignitable corrosive reactive or toxic D listed e Listed Wastes includes wastes from common manufacturing and industrial processes F listed wastes from specific industries K listed and wastes from commercial chemical products U and P listed e Universal Waste includes certain batteries primarily rechargeable batteries such lithium nickel cadmium nickel metal hydride and mercury oxide mercury containing equipment e g thermometers thermostats and certain lamps e g fluorescent bulbs Note alkaline batteries and incandescent bulbs are not considered Universal Wastes and can be legally disposed of as trash e Mixed Waste hazardous waste mixed with radioactive waste EPA regulated hazardous waste should not be mistaken for biological or radiological wastes A more detailed definition of hazardous waste including the D F P and U lists is provided in Appendices 1 and 2 of the Hazardous W
107. ator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile and latex disposable gloves are NOT suitable Viton gloves are recommended Check the resources below for a more suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Benzene Refer to glove selection chart from the links below Benzene Date 8 19 2013 PURDUE UNIVERSITY http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical s
108. azard 4 Deadly 4 FP lt 73 F 4 May Detonate ACID Acid 3 Extreme Danger 3 FP lt 100 F 3 Shock Heat May ALK Alkali 2 Hazardous 2 100 F lt FP lt 200 F Detonate COR Corrosive 1 Slightly Hazardous 1 FP gt 200 F 2 Violent Chemical Ww 0 Normal Material 0 Will Not Burn Change 8 1 Unstable if Heated FP Flash Point 0 Stable No Water Radioactive Figure 2 4 NFPA Hazard Rating System 2 3 Department of Transportation Hazard Classes The DOT regulates the transportation of all hazardous materials in the United States and defines a hazardous material as any substance that has been determined to be capable of posing an unreasonable risk to health safety or property when transported in commerce There are several methods that can be employed to determine whether a chemical is hazardous for transport a few of which included e Reviewing the DOT Hazardous Materials Table 49 CFR 172 101 e Reviewing the SDS specifically Section 2 Hazardous Identification and Section 14 Transport Considerations for the chemical being shipped as detailed above in Section 2 1 1 of the CHP 14 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems e Reviewing the chemical label and looking for hazard information detailed above in Section 2 1 2 of the CHP and e Understanding the chemical and physical properties of the chemical All ha
109. azardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Flammable solid waste should be segregated from all incompatible chemicals and sources of ignition Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Flammable Solids Date 11 13 2013 PURDUE UNIVERSITY Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific flammable solid being used must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with flammable solids designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and eme
110. be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Rinse immediately contaminated clothing and skin with plenty of water before removing clothes Section 6 Engineering Controls Use of Bromine should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Bromine Date 2 17 2014 PURDUE UNIVERSITY Section 8 Special Handling and Storage Require
111. before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes Seek immediate medical attention and continue eye rinse during transport to hospital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Consult a physician Section 8 Special Handling and Storage Requirements e Conduct the procedure only after a supervisor has observed the user performing the proper technique unassisted Diethyl Ether Date 8 19 2013 PURDUE UNIVERSITY e Labelall diethyl ether containers with the purchase date and the date opened Storage time should never exceed the manufacturer s recommendations The general storage time limit for diethyl ether is 12 months Keep away from sources of ignition Avoid heat and shock or friction when handling Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances Store away from light Avoid contact with skin eyes and inhalation Diethyl ether must be segregated from incompatible materials such as oxidizers strong acids halogens and sulfur compounds The amount of diethyl ether stored should be kept at an absolute minimum Prevent electric static build up with a grounding cable when transferring diethyl ether Suitable storage locations include flammable storage cabinets or i
112. ble chemical storage locations may include flammable cabinets corrosive cabinets laboratory shelves or appropriate laboratory refrigerators or freezers Chemical containers must be in good condition and appropriate for the chemical that they contain and be free from exterior contamination Fume hoods should not be used as permanent chemical storage areas unless designated as such Not only does this create potentially unsafe conditions by having extraneous chemicals stored near chemical reactions and processes excess chemical 34 The official version of this document will only be maintained online 5 7 2 Chapter 5 Laboratory Management Plan bottles in the hood may also seriously impair the ventilating capacity of the hood Only chemicals being used in the process or experiment being conducted in the hood are allowed to be stored in the hood and should be removed when the process or experiment is complete Chemicals should not be permanently stored on bench tops Avoid storing any chemical containers on the floor Under no circumstance should chemical containers or anything else be stored in aisle ways corridors or in front of doors Hazardous liquids should not be stored on shelves above eye level unless there is a SOP detailing safe handling procedures Chemicals should be stored at an appropriate temperature and humidity level and never be stored in direct sunlight Periodic cleanouts of expired or unneeded chemicals should be
113. ble exposure limit for an OSHA 67 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy regulated substance for which there are exposure monitoring and medical surveillance requirements medical surveillance shall be established for the affected employee as prescribed by the particular standard and Whenever an event takes place in the work area such as a spill leak explosion or other occurrence resulting in the likelihood of a hazardous exposure the affected employee shall be provided an opportunity for a medical examination All medical examinations must be performed by or under the direct supervision of a licensed medical care provider and must be provided without cost to the employee 68 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management Chapter 7 Hazardous Waste Management 7 1 Introduction Hazardous waste is generally defined as waste that is dangerous or potentially harmful to human health or the environment Hazardous waste regulations are strictly enforced by both the Environmental Protection Agency EPA and the Indiana Department of Environmental Management The laboratory supervisor is responsible for managing the hazardous waste program in a safe and compliant manner No chemical waste should be poured down the drain or discarded in the trash unless it is certain that doing so does not violate hazardous waste regul
114. bryotoxins include thalidomide and certain antibiotics such as tetracycline Women of childbearing potential should note that embryotoxins have the greatest impact during the first trimester of pregnancy Because a woman often does not know that she is pregnant during this period of high susceptibility special caution is advised when working with all chemicals especially those rapidly absorbed through the skin e g formamide 92 The official version of this document will only be maintained online B 2 5 3 Appendix B OSHA Hazard Class Definitions Substances with a High Acute Toxicity Substances that have a high degree of acute toxicity are materials that may be fatal or cause damage to target organs as the result of a single exposure or exposures of short duration Acute toxins are quantified by a substance s lethal dose 50 LDso or lethal concentration 50 LC o which is the lethal dose of a compound to 50 of a laboratory tested animal population e g rats rabbits over a specified time period High acute toxicity includes any chemical that falls within any of the following OSHA defined categories A chemical with a median lethal dose LD50 of 50 mg or less per kg of body weight when administered orally to certain test populations A chemical with an LD50 of 200 mg less per kg of body weight when administered by continuous contact for 24 hours to certain test populations A chemical with a median lethal concent
115. cabinets are not designed to be used with chemical applications so the use of chemicals should be kept to a minimum Applications that involve the use of chemicals should be conducted in chemical fume hoods Figure 4 5 Biological Safety Cabinet 4 2 5 Safety Showers and Eyewash Stations All laboratories using hazardous chemicals must have immediate access to safety showers and eye wash stations Safety showers must have a minimum clearance of 24 inches from the centerline of the spray pattern in all directions at all times Identify the safety station with a highly visible sign and maintain an unobstructed path to it All lab personnel must be aware of the location and know how to properly use the safety shower and eyewash stations If lab personnel are exposed to a hazardous chemical they should dial 911 or someone else in the lab that is not exposed should dial 911 and use the safety shower and or eye wash unit for 15 minutes or until emergency response have personnel arrive and begin treatment If an uninjured individual is present this person should assist with the decontamination of the affected individual All eyewash stations must be flushed by laboratory personnel on a weekly basis to ensure proper working order This will keep the system free of sediment and prevent bacterial growth from reducing performance REM performs annual inspections of all campus safety shower and eyewash stations This inspection evaluates the basic mecha
116. cals Laboratory personnel should use the SDS as a resource to obtain information about hazards and safety precautions SDSs cannot provide information for hazards in all circumstances However the SDS information enables the employer to develop an active program of worker protection measures such as training on hazard mitigation Chemical manufacturers are required to use a standard format when developing SDSs The SDS will contain 16 headings which are illustrated in Figure 2 1 9 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Identification of the substance or a 1 f 9 Physical and chemical properties mixture and of supplier 2 Hazards Identification 10 Stability and reactivity C iti inf ti o P pa ISRAINTORRSTISH on 11 Toxicological information ingredients 4 First aid measures 12 Ecological information 5 Firefighting measures 13 Disposal considerations 6 Accidental release measures 14 Transport considerations 7 Handling and storage 15 Regulatory information Exposure controls personal f 8 16 Other information protection Figure 2 1 GHS Required Sections of a Safety Data Sheet 2 1 2 Chemical Labeling The GHS standardized label elements which are not subject to variation and must appear on the chemical label contain the following elements e Symbols hazard pictograms
117. cccccssssccccssssscccccssssceccecssceccecsssecsceessseees 86 Bil Physical Hazard Sieniniai ann naar le bu en 87 iii The official version of this document will only be maintained online Table of Contents B 1 1 Elammable liquids 22 20220 220 Ran wen seen 87 B 12 Fl mmablesolldsA n ets a ee ale eine ee 87 B 13 Gases under Pressure ausser Lernen 88 B 1 4 Pyrophoric Self Heating and Self Reactive Materials ueneesnseessssnsnneensennnnennnennnn 88 B 1 5 W ter Reactive Materials nn ae 89 B 16 Oxidizer S ne ERNEST ee 89 B17 Organic Peroxides 2 2 a essen a a T Ea EE 89 B 41 8 EXOIOSIVES ar a a totaal ce ARE Ra e A E ee 90 Bed Health Hazard anne rauen 90 B 2 1 UIItants cccivecesstivieesssiieveseviveseccdovevecevaveieused ever eseveveuai ovens evaveueus EA E 90 Bi2 2 Sensitizersauanensr ini tas E N teva das E EAE E ea seiaiaeeabee ees es 90 Bi2 23 COMOSIVES 8 ninata an na Ae ete eet eas sa len 91 B 2 4 Hazardous Substances with Toxic Effects on Specific Organs cccessssceeeeeeeeeees 91 B 2 5 Particularly Hazardous Substances ccccccesesssssscececeescessesseaeeeceeeesssessaeeeseesesseeeees 91 B 2 5 1 CarcimOBens a kaa a a sess aks Ade ohh A R A te aaa ieee E ER ai 92 B 2 5 2 Reproductie TOA Sa aa aa a Laa AAAA AAE A AA Ea AERONA 92 B 2 5 3 Substances with a High Acute Toxicity cccccccccessssssssceceeeeeesssessaeeeeeeeeeeseeees 93 Appendix C Peroxide Forming Chemicals
118. ccupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Rinse immediately contaminated clothing and skin with plenty of water before removing clothes Section 6 Engineering Controls Use of corrosives should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Corrosives Date 10 1 2013 PURDUE UNIVERSITY Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Section 8 Special Handling and Storage Requirements e Do not over purchase only p
119. ch other Segregation can be achieved either by physical distance preferred method or by secondary containment as shown in Figure 5 6 Acetic Acid Figure 5 6 Segregation Using Secondary Containment 5 7 7 Oxidizers and Organic Peroxide Storage Oxidizing materials are defined as substances which while in itself are not necessarily combustible may generally by yielding oxygen cause or contribute to the combustion of other material An organic peroxide is an organic substance which contains the bivalent O 39 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan O structure and may be considered a derivative of hydrogen peroxide where one or both of the hydrogen atoms have been replaced by organic radicals The following guidelines for storing oxidizers and organic peroxides must be followed in all laboratories e Oxidizers e g hydrogen peroxide sodium nitrate and organic peroxides e g methyl ethyl ketone peroxide benzoyl peroxide must be stored in a cool dry location and kept away from combustible materials such as wood pressboard paper and organic chemicals e g organic solvents and organic acids e f possible store all strong oxidizing agents in a chemical cabinet dedicated only for oxidizers e The amount of oxidizers and organic peroxides stored in the lab should be kept at a minimum e All material must be clearly labeled the original manufacturer
120. cident it is recommended that a second trained individual be present in the lab or in the vicinity Store Sodium azide in secondary containment with Acute Toxin label on the primary container secondary containment and the storage location Do not store on metal shelves or use metal items spatulas to handle sodium azide Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such as metals acids Carbon disulfide Bromine Chromyl chloride Sulfuric acid Nitric acid Hydrazine and Dimethyl sulfate e Avoid contact with skin eyes and inhalation Avoid formation of dusts and aerosols Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the ey
121. ckets tubes and rotors properly e Check that the rotor is seated on the drive correctly close the lid on the centrifuge and secure it e When using swinging bucket rotors make sure that all buckets are hooked correctly and move freely During centrifugation e Close lids at all times during operation Never open a centrifuge until the rotor has stopped e Do not exceed safe rotor speed e The operator should not leave the centrifuge until full operating speed is attained and the machine appears to be running safely without vibration e Stop the centrifuge immediately if an unusual condition noise or vibration begins and check load balances 45 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan After centrifugation e Allow the centrifuge to come to a complete stop before opening e Wear new pair of outer gloves to remove rotor and samples e Check inside of centrifuge for possible spills and leaks clean centrifuge and rotor thoroughly if necessary e Wash hands after removing gloves 5 12 2 Stirring and Mixing Equipment Stirring and mixing devices commonly found in laboratories include stirring motors magnetic stirrers and shakers These devices are typically used in lab operations that are performed in a chemical fume hood and it is important that they be operated ina way that prevents the generation of electrical sparks Only spark free induction motors
122. compounds At a minimum the Laboratory Supervisor should be able to determine if a chemical is flammable corrosive oxidant or reactive Call REM at 765 765 49 40121 for assistance with identifying the hazards of samples If samples are consolidated for storage e g vial boxes it is not always necessary to label every sample container For example a box containing sample vials which are all in the same hazard class e g miscellaneous pharmaceutical compounds considered to be toxic can have one label on the outside of the box stating Miscellaneous Toxic Pharmaceutical Compounds or a similar description A label such as the one shown in Figure 5 9 can be used to identify consolidated samples and should only be used on a temporary basis This type of information communicates the hazards to emergency responders as well as gives REM the information necessary for proper disposal If the chemical substance is produced for another user outside of the lab the Laboratory Supervisor must comply with the Hazard Communication Standard including the requirements for preparation of SDSs and container labeling Chemical Description isee lan Employee Name Date 10 10 2013 Circle the hazard s that apply Flammable Reactive Oxidizer Corrosive Explosive Irritant Non Haz Figure 5 9 Example Temporary Sample Container Label 5 14 Transporting Hazardous Chemicals Transporting chemicals is a potentially hazardous process
123. copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 7664 93 9 GHS Classification Corrosive Molecular Formula H SO Form physical state Liquid Color Clear colorless to slightly yellow Boiling Point 290 C 554 F Sulfuric acid Date 3 19 2014 PURDUE UNIVERSITY Flash Point N A Relative Vapor Density 3 39 Uses Sulfuric acid alternative spelling Sulphuric acid is a highly corrosive mineral acid The historical name of this acid is oil of vitriol Possessing different chemical properties Sulfuric acid has a wide range of applications including domestic acidic drain cleaner electrolyte in lead acid batteries and various cleaning agents It is also a central substance in the chemical industry Principal uses include mineral proc
124. cords cables and extension cords may only be used during and for renovation maintenance repair or experimental work The following additional requirements apply e Ground fault protection e g GFCI must be provided on all temporary wiring circuits including extension cords used for construction or maintenance activities e In general all equipment and tools connected by cord and plug must be grounded Listed or labeled double insulated tools and appliances need not be grounded e Receptacles must be of the grounding type e Flexible cords and cables must be of an approved type and suitable for the location and intended use They may not be used as a substitute for the fixed wiring where run through holes in walls ceilings or floors where run through doorways windows or similar openings where attached to building surfaces or where concealed behind building walls ceilings floors rugs or carpeting e Suitable disconnecting switches or plug connects must be installed to permit the disconnection of all ungrounded conductors of each temporary circuit e Lamps for general illumination must be protected from accidental contact or damage either by elevating the fixture above 8 feet above the floor or other working surface or by providing a suitable guard Hand lamps supplied by flexible cord must be equipped with a handle of molded composition or other approved material and must be equipped with a substantial bulb guard e Flexible cords
125. ct Inhalation of solvent vapors may cause chronic toxic effects in the liver or kidney Contact with skin causes burns and irritation Prolonged or repeated skin exposure may cause skin defatting or dermatitis Eye contact causes burns irritation and may cause blindness Ingestion may cause permanent damage to the digestive tract Flash fires may occur in the presence of ignition sources ae et Section 5 Personal Protective Equipment PPE Respirator Protection If base baths are being used outside of a chemical fume hood respiratory protection may be required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Respirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal
126. ction and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone 765 494 4573 Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP Building Room Number Section 2 Type of SOP CI Process L Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A GHS Classification Flammable solid Many are also Irritant or Toxic Molecular Formula N A Form physical state Solid Color N A Flammable Solids Date 11 13 2013 PURDUE UNIVERSITY Boiling point N A Section 4 Potential Hazards A flammable solid is a solid which is readily combustible or may cause or contribute to a fire through friction Readily combustible solids are powdered granular or pasty substances which are dangerous if they can be easily ignited by brief contact with an ignition source Flammable solids are more hazardous when widely dispersed in a confined space e g finely divided metal powders Heating may ca
127. ctioning chemical fume hood Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended For extended periods of usage wearing two pairs of nitrile gloves is recommended NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Ethidium bromide Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes Ethidium Bromide Date 7 19 2013 PURDUE UNIVERSITY must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of powdered form pur
128. ctric current e Ifa heating device becomes so worn or damaged that its heating element is exposed the device should be either discarded or repaired before it is used again e The external cases of all variable autotransformers have perforations for cooling by ventilation and therefore should be located where water and other chemicals cannot be spilled onto them and where they will not be exposed to flammable liquids or vapors e Fail safe devices can prevent fires or explosions that may arise if the temperature of a reaction increases significantly because of a change in line voltage the accidental loss of reaction solvent or loss of cooling Some devices will turn off the electric power if the temperature of the heating device exceeds some preset limit or if the flow of cooling water through a condenser is stopped owing to the loss of water pressure or loosening of the water supply hose to a condenser 5 12 4 Distillation and Solvent Purification Systems The process of thermal solvent distillation is inherently dangerous If not handled properly fire explosion and or personnel exposure can result A few common chemicals distilled in laboratories include tetrahydrofuran methylene chloride diethyl ether toluene dimethylformamide benzene and hexanes The guidelines below should be followed while thermal distillation of organic solvents is conducted in the lab e The thermal solvent distillation system should be installed inside o
129. cupying the laboratory area The area of skin between the shoe and ankle must not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of Methylene chloride must be conducted in a properly functioning chemical fume hood The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Consult a physician In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes Seek immediate medical attention and continue eye rinse during transport to hospital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Consult a physician Methylene Chloride Date 9 26 2013 PURDUE UNIVERSITY Section 8 Special Handling and Storage Requirements e A designated storage area must be established for Methylene chloride and the area should be posted with a Caution Carcino
130. d 2 The official version of this document will only be maintained online Chapter 1 Introduction 4 Protective laboratory practices and equipment are made available and in common use to minimize the potential for employee exposure to hazardous chemicals The CHP was prepared in accordance with the requirements of the Occupational Safety and Health Administration OSHA Occupational Exposure to Hazardous Chemicals in Laboratories Standard Lab Standard found in 29 CFR 1910 1450 and is based on best practices identified in among other sources the Global Harmonized System of Classification and Labeling of Chemicals Prudent Practices for Handling Hazardous Chemicals in Laboratories published by the National Research Council the American Chemistry Society Task Force on Laboratory Chemical and Waste Management s Laboratory Waste Management A Guidebook the Princeton University Laboratory Safety Manual and the University of California Los Angeles Chemical Hygiene Plan 1 3 CHP Use Instructions The information presented in the CHP represents best practices and provides a broad overview of the information necessary for the safe operation of laboratories that utilize hazardous chemicals It is not intended to be all inclusive Departments engaged in work with hazardous chemicals or hazardous operations that are not sufficiently covered by the CHP must customize this document by adding appropriate sections in
131. d 2 i Danger Boiling point gt 35 C and vapor i Flammable liquid and 3 Flash point gt 23 C and lt 60 C Warning vapor 4 Flash point gt 60 C and lt 93 C Warning Combustible liquid 5 There is no Category 5 for flammable liquids 2 2 National Fire Protection Association Rating System The NFPA system uses a diamond shaped diagram of symbols and numbers to indicate the degree of hazard associated with a particular chemical This system was created to easily and quickly communicate hazards to first responders in the event of an emergency situation These diamond shaped symbols are placed on chemical containers to identify the degree of hazard associated with the specific chemical or chemical mixture The NFPA system is acommon way to identify chemical hazards and should be understood by laboratory employees The NFPA 704 numerical rating system is based on a0 4 system 0 meaning no hazard and 4 meaning the most hazardous note this in contrast to the GHS system where 1 is the most hazardous and 4 is the least hazardous Figure 2 4 illustrates the NFPA hazard rating system and identifies both the hazard categories and hazard rating system 13 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Fire Hazard Health Hazard Reactivity Hazard ACID Specific Hazard Health Hazard Fire Hazard Reactivity Hazard Specific H
132. d Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Flammable Solids Date 11 13 2013 PURDUE UNIVERSITY Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Face shield is also recommended Skin and Body Protection Flame resistant lab coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of flammable solids must be conducted in a chemical fume hood The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Seek medical attention immediately In case of skin contact Immediately flush skin with plenty of water for
133. d administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended Wearing two pairs of nitrile gloves is recommended NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific alkali metal being handled Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Alkali Metals Date 9 26 2013 PURDUE UNIVERSITY Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Face shield is also recommended Skin and Body Protection Flame resistant lab coats must be worn and be appropriately sized for the individual
134. d with careful consideration of the potential risks The following guidelines should be followed when using vacuum apparatus in the laboratory e Donot allow water solvents or corrosive gases to be drawn into vacuum systems Protect pumps with cold traps and vent their exhaust into an exhaust hood e Assemble vacuum apparatus in a manner that avoids strain particularly to the neck of the flask e Avoid putting pressure on a vacuum line to prevent stopcocks from popping out or glass apparatus from exploding e Place vacuum apparatus in such a way that the possibility of being accidentally hit is minimized If necessary place transparent plastic around it to prevent injury from flying glass in case of an explosion e When using a rotary evaporator the glass components of the rotary evaporator should be made of Pyrex or similar glass Glass vessels should be completely enclosed in a shield to guard against flying glass should the components implode Increase in rotation speed and application of vacuum to the flask whose solvent is to be evaporated should be gradual e When using a vacuum source it is important to place a trap between the experimental apparatus and the vacuum source The vacuum trap protects the pump and the piping from the potentially damaging effects of the material protects people who must work on the vacuum lines or system and prevents vapors and related odors from being emitted back into the laboratory or system e
135. defective cord or cord and plug connected equipment must be removed from service and no person may use it until it is repaired and tested to ensure it is safe for use Extension cords must be of the three wire type Extension cords and flexible cords must be designed for hard or extra hard usage The rating or approval must be visible Portable equipment must be handled in a manner that will not cause damage Flexible electric cords connected to equipment may not be used for raising or lowering the equipment Extension cords must be protected from damage Sharp corners and projections must be avoided Flexible cords may not be run through windows or doors unless protected from damage and then only on a temporary basis Flexible cords may not be run above ceilings or inside or through walls ceilings or floors and may not be fastened with staples or otherwise hung in such a fashion as to damage the outer jacket or insulation Extension cords used with grounding type equipment must contain an equipment grounding conductor the cord must accept a three prong or grounded plug Operating equipment with extension cords without a grounding plug is prohibited Attachment plugs and receptacles may not be connected or altered in any way that would interrupt the continuity of the equipment grounding conductor Additionally these devices may not be altered to allow the grounding pole to be inserted into current connector slots Clipping the grounding prong
136. dioxide toxicity nitric oxide flammable propane and corrosive hydrogen chloride Chapter 5 7 3 of the CHP details compressed gases storage requirements B 1 4 Pyrophoric Self Heating and Self Reactive Materials Pyrophoric material also called spontaneously combustible is a liquid or solid that even in small quantities and without an external ignition source can ignite after coming in contact with the air ee Self heating material is a solid or liquid other than a pyrophoric substance which by reaction with air and without energy supply is liable to self heat This endpoint differs from a pyrophoric substance in that it will ignite only when in large amounts kilograms and after long periods of time hours or days 88 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions Self reactive material is a thermally unstable liquid or solid liable to undergo a strongly exothermic thermal decomposition even without participation of oxygen air Chapter 5 7 4 of the CHP details the storage requirements for reactive chemicals B 1 5 Water Reactive Materials A water reactive material is a liquid or solid that reacts violently with water to produce a flammable or toxic gas or other hazardous conditions Alkali metals e g sodium potassium and metal hydrides e g calcium hydride are common water reactive materials found in laboratories Chapter 5 7
137. e Base bath solutions cannot be disposed of down the drain Do not mix base bath solutions with acidic waste streams After the base bath has lost its cleaning effectiveness transfer the solution to a heavy duty container such as Nalgene bottle carboy Make sure the waste container s is properly labeled label should indicate all of the contents of the container including any potential organic contaminants from cleaning process REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area away from incompatible materials Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific base bath solution being used must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additional lab protocol may be added here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Sect
138. e e All peroxide forming solvents should be tested prior to distillation e Peroxide forming solvents should be purchased in limited quantities e Peroxide forming solvents should be marked with the purchase date and the date opened e Peroxide forming solvents should be sealed tightly and stored away from light and heat e Periodic testing should be done on opened containers and the results marked on the containers Testing e Obtain test strips for the range of 0 100 ppm peroxide e Record the test results on the bottle e Ifthe test results are 100 ppm or greater contact REM at 765 494 0121 for proper disposal 96 The official version of this document will only be maintained online Tab 1 Specific Standard Operating Procedures Tab 1 Specific Standard Operating Procedures PURDUE UNIVERSITY Standard Operating Procedure Alkali Metals This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone
139. e Flammable Liquid Corrosive majority of pyrophorics have these hazards Molecular Formula N A Form physical state N A Color N A Pyrophoric Materials Date 9 18 2013 PURDUE UNIVERSITY Boiling point N A Section 4 Potential Hazards Pyrophoric materials are classified as pyrophoric and typically also classified as flammable liquids solids water reactives and corrosives Many are often toxic They react violently with water liberating extremely flammable gas Spontaneously flammable in air and causes burns Dangers of serious damage to health by prolonged exposure through inhalation Extreme caution is advised Keep away from heat and sources of ignition While it is often possible to work with these compounds using cannula transfer traces of these compounds at the tip of the needle or cannula may catch fire and clog the cannula with lithium salts Some workers prefer to enclose the needle tip or cannula in a short glass tube which is flushed with an inert gas and sealed via two septa Examples include tert butyllithium and triethylaluminum Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulatio
140. e ethidium bromide must be conducted in a properly functioning chemical fume hood The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Seek medical attention immediately In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Seek medical attention immediately In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention and continue eye rinse during transport to hospital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek medical attention immediately Section 8 Special Handling and Storage Requirements Designate an area where ethidium bromide work is going to occur and only handle it in this area i e chemical fume hood bench top The area should be posted with a Caution Carcinogen Reproductive Toxins or Extremely Toxic Chemicals label provided by REM as shown to the right It is recommended that the surfaces of the designated
141. e hazard labels and pictograms The label should not be defaced in any way All pyrophoric material should be placed into secondary containment as a precautionary measure Suitable storage locations include inert gas filled desiccators or glove boxes flammable storage cabinets that do not contain aqueous or other incompatible chemicals or intrinsically safe refrigerators or freezers that also do not contain aqueous or other incompatible chemicals If pyrophoric materials are received in a specially designed shipping storage or dispensing container such as the Aldrich Sure Seal packaging system ensure that the integrity of that container is maintained Ensure that sufficient protective solvent oil kerosene or inert gas remains in the container while pyrophoric materials are stored Additional Resources e Aldrich Technical Bulletins AL 134 and AL 164 e Handling Pyrophoric Reagents from U S Dept of Energy and Pacific Northwest National Laboratory e Chemical Hygiene and Safety Plan from Berkeley National Laboratory Lithium alkyls Date 2 1 2013 PURDUE UNIVERSITY Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Once spilled all liquid or solid pyrophoric chemicals may instantly ignite Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor a
142. e Management Figure 7 2 Container Failure Due to Mixing Incompatible Waste Streams 7 4 Waste Containers REM does not provide containers to campus It is the responsibility of the generator of the waste to provide containers Usually the original container of the main component of the waste can be used e g 4 liter glass jar 5 gallon green metal solvent can Purdue Stores also offers waste containers such as 20 liter carboys as shown in Figure 7 3 for sale If requested reusable hazardous waste storage containers of 5 gallons or larger may be returned to the generator s area Mark the container clearly with Return to the building and room number as illustrated in Figure 7 4 Containers unsuitable for reuse will be properly disposed of and not returned 72 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management Figure 7 3 20 Liter Carboy Figure 7 4 Reusable Waste Container Purdue s policy for the disposal of empty containers is implemented to protect Purdue facilities and the Physical Facilities Buildings and Grounds staff when removing trash Please remember that some chemical residues have the potential to mix with other incompatible residues in the dumpster or compactor causing a reaction or fire In addition sealed containers may become pressurized during compaction which may result in residues spraying onto workers Please keep the following procedures and
143. e Solids A flammable solid is a solid which is readily combustible or may cause or contribute to a fire through friction Readily combustible solids are powdered granular or pasty substances which 87 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions are dangerous if they can be easily ignited by brief contact with an ignition source Flammable solids are more hazardous when widely dispersed in a confined space e g finely divided metal powders B 1 3 Gases under Pressure Gases under pressure are gases which are contained in a receptacle at a pressure not less than 280 kPA at 20 C or as a refrigerated liquid Gases under pressure include the following e Compressed gas is a gas which when packaged under pressure is entirely gaseous at 50 C including all gases with a critical temperature lt 50 C e Liquefied gas is a gas which when packaged under pressure is partially liquid at temperatures above 50 C e Refrigerated liquefied gas is a gas which when packaged is made partially liquid because of its low temperature e Dissolved gas is a gas which when packaged under pressure is dissolved in a liquid phase solvent All compressed gases are hazardous due to the fact they are stored in compressed cylinders which can explode and act as a projectile if ruptured Compressed gases also carry the hazards of the chemicals they contain such as asphyxiation carbon
144. e files contlabl htm 4 4 Personal Protective Equipment PPE Personal protective equipment PPE should be used to supplement engineering controls However PPE should never be used as a substitute for engineering controls when engineering controls are required PPE must be worn at all time in the laboratory when handling hazardous chemicals Proper PPE selection can be determined in the following ways e Ask the Laboratory Supervisor about proper PPE selection e Review the SOP and associated hazard assessment for the task to be performed e Review Section 8 Exposure Controls Personal Protection of the SDS for the chemical s being used This will provide basic information on the PPE recommended for use with the particular chemical The SDS addresses worst case conditions therefore all the equipment described may not always be necessary for a specific job In addition the SDS may not provide sufficient information concerning a specific respirator or type of glove appropriate for the chemical Additional PPE requirements are detailed in the Laboratory PPE Policy in Chapter 6 and Chapter 9 of the CHP 28 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Chapter 5 Laboratory Management Plan An effective laboratory management plan is essential to operating a safe lab environment Requirements on topics such as lab housekeeping chemical inventories proper handling stor
145. e g hexane ethyl acetate xylene are more hazardous at elevated temperatures due to more rapid vaporization The following definitions are important to understand when evaluating the hazards of flammable liquids e Flammable liquid is a liquid having a flash point no greater than 93 C 200 F e Flash point is the minimum temperature at which the application of an ignition source causes the vapors of a nega liquid to ignite under specified test conditions e Boiling point is the temperature at which the vapor pressure of a liquid equals the atmospheric pressure and the liquid changes into a vapor e Auto ignition temperature is the minimum temperature at which self sustained combustion will occur in the absence of an ignition source e Lower explosive limit LEL is the lowest concentration percentage of a gas or a vapor in air capable of producing a flash of fire in presence of an ignition source arc flame heat 0 GAS 100 AIR e Upper explosive limit UEL is the highest concentration percentage of a gas or a vapor in air capable of producing a flash of fire in presence of an ignition source arc flame heat Some organic solvents e g diethyl ether have the potential to form potentially shock sensitive organic peroxides See Appendix 3 for additional information regarding peroxide forming chemicals Chapter 5 7 2 of the CHP details flammable liquids storage requirements B 1 2 Flammabl
146. e of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended HF waste should be segregated from all incompatible chemicals such as caustics Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the nitric acid SDS must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with nitric acid designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS p
147. e open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area Sodium Azide Date 3 27 2014 PURDUE UNIVERSITY Dispose of Waste Pure Sodium azide or solutions containing Sodium azide are not permitted to be poured down the drain Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for Sodium azide must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Pr
148. e toxic effects on the nervous system such as mercury acrylamide and carbon disulfide e Substances that act on the hematopoietic system e g carbon monoxide and cyanides which decrease hemoglobin function and deprive the body tissues of oxygen e Substances that damage lung tissue such as asbestos and silica B 2 5 Particularly Hazardous Substances Substances that pose such significant threats to human health are classified as particularly hazardous substances PHSs The OSHA Laboratory Standard requires that special provisions be established to prevent the harmful exposure of researchers to PHSs including the establishment of designated areas for their use Particularly hazardous substances are divided into three primary types 1 Carcinogens 91 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions 2 Reproductive Toxins 3 Substances with a High Acute Toxicity B 2 5 1 Carcinogens Carcinogens are chemical or physical agents that cause cancer Generally they are chronically toxic substances that is they cause damage after repeated or long duration exposure and their effects may only become evident after a long latency period Chronic toxins are particularly insidious because they may have no immediately apparent harmful effects These materials are separated into two classes 1 Select Carcinogens Select carcinogens are materials which have met certain criteria
149. each health hazard e Carcinogens Substances that cause cancer Generally they are chronically toxic substances that is they cause damage after repeated or long duration exposure and their effects may only become evident after a long latency period Carcinogens are separated into two classes select carcinogens and regulated carcinogens e Corrosives Substances that cause destruction of living tissue by chemical corrosion at the site of contact and can be either acidic or caustic basic e Hazardous Substances with Toxic Effects on Specific Organs Substances that pose adverse health effects to specific organs such as the liver kidneys lungs etc e High Acute Toxicity Substances Substances that may be fatal or cause damage to target organs as the result of a single exposure or exposures of short duration Acute toxins are quantified by a substance s lethal dose 50 LD50 or lethal concentration 50 LC50 which is the lethal dose of a compound to 50 of a laboratory tested animal population e g rats rabbits over a specified time period e Irritant Substances that cause reversible inflammatory effects on living tissue by chemical action at the site of contact e Reproductive Toxins Substances that may affect the reproductive capabilities including chromosomal damage mutations and effects on fetuses teratogens e Sensitizer also called allergen A substance that causes exposed individuals to develop an allergic reaction in no
150. ed Acutely toxic materials should be stored in secondary containment at all times as a best management practice If possible store all acutely toxic materials in a cabinet dedicated only for acutely toxic materials Signs should be posted to indicate their presence and unique hazards 38 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e The amount of acutely toxic material stored in the lab should be kept at a minimum Any expired or unnecessary materials must be properly disposed of as hazardous waste e All acutely toxic materials should be clearly labeled with the original manufacturer s label which should have the chemical name hazard labels and pictograms The label should not be defaced in any way 5 7 6 Corrosive Materials Storage Corrosive materials are defined as substances that cause destruction of living tissue by chemical corrosion at the site of contact and can be either acidic or basic caustic The best storage method for corrosive materials is inside of a corrosive storage cabinet or lab cabinet where acids and bases are segregated at all times Acids must also be segregated from chemicals where a toxic gas would be generated upon contact with an acid e g reactive cyanide compounds Organic acids e g acetic acid formic acid must be stored away from oxidizing acids e g nitric acid perchloric acid as these types of acids are incompatible with ea
151. ed for storage samples movement of samples between campus locations or for shipments off campus for collaborative research Figure 6 There is no pressure relief device on a cryogenic tube other than the lid Consequently cryogenic tubes can explode without warning Explosions are likely caused by trapped nitrogen expanding inside of the tube during the thawing process As the temperature increases the tube may become over pressurized and explode and may result in serious injuries Because of this risk the PPE illustrated in Figure 2 should be worn when directly handling a sealed cryogenic tube Figure 6 Cryo Tubes Self Pressurizing Tanks Self pressurizing tanks Figure 7 are generally a 140 260 L double wall stainless steel tank used to fill other liquid nitrogen containers such as Dewars These tanks are equipped with pressure relief valves and a backup rupture disk A loud hissing sound is commonly heard when the pressure relief valve opens Exposure to liquid nitrogen can occur when connecting and disconnecting equipment during the filling process from a leaking valve or from condensate ice buildup on valves and hoses Because of these risks the PPE illustrated in Figure 3 should always be worn when working with a self pressurized tank Section 8 General Safe Handling Practices and Storage Requirements Figure 7 Self Pressurizing Tank e Only trained personnel should work with liquid nitrogen e Use only in well ven
152. ed in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Piranha Solution Date 3 14 2014 PURDUE UNIVERSITY Standard Operating Procedure Potassium Cyanide This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014
153. ed with the original manufacturer s label which should have the chemical name hazard labels and pictograms The label should not be defaced in any way Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides h
154. edicated only for reactives Signs should be posted to indicate their presence and unique hazards as shown in Figure 5 5 37 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan SS en PY ROPHORICS Figure 5 5 Reactive Chemicals Storage Many reactive materials are water and or air reactive and can spontaneously ignite on contact with air and or water Therefore reactives must be handled under an inert atmosphere and in such a way that rigorously excludes air and moisture If reactive materials are received in a specially designed shipping storage or dispensing container such as the Aldrich Sure Seal packaging system ensure that the integrity of that container is maintained Ensure that sufficient protective solvent oil kerosene or inert gas remains in the container while reactive materials are stored 5 7 5 Acutely Toxic Materials Storage Acutely toxic materials are defined as substances that may be fatal or cause damage to target organs as the result of a single exposure or exposures of short duration The following guidelines for storing acutely toxic materials must be followed in all laboratories Suitable storage locations for acutely toxic materials include desiccators glove boxes flammable storage cabinets that do not contain incompatible chemicals primarily strong acids or non domestic refrigerators or freezers These locations should be clearly post
155. eesesseeeeea 44 5 12 Equipment Apparatus and Instrument Safety ccccccccccecessssssseceeeeseessessneeeeeesesseeeees 45 512 1 Gentrif ges 2 23 080 E A A ae ease veep eas odie ses A heeds 45 5 12 2 Stirring and Mixing Equipment ueseneeessssssnsnenensnnennnnnnnnnonnnnnnnennnnsnnnnnnnnnnnensse nennen 46 5212 3 Heat ng Devices nan aa man Rare in allen nee aeg 46 5 12 4 Distillation and Solvent Purification Systems 2ussssesensnsensennnnsnenennnnnnnennsnnnenenn 47 5 12 5 Laboratory Glassware ccccccccssssscccecececsesesssesececeescsesesaeaeeeseeseesseseaeseeeesesseessessaees 48 5 12 6 High Pressure Systems Sss sarria rir i e EE AE E E E T a i aaa 48 1227 2VACUUMISYSEOIMS iena eaer ea e e a e a a a e a a ideas 49 5 13 Research Samples and Chemicals Developed in the Lab ccccccccccccsssesssseeeeeeeeeeeeees 50 5 14 Transporting Hazardous Chemicals ccccssssececssssececeeseececeesseeeceeseaeeeesesaeeeessseeaeeeeeeaaes 51 5 14 1 Shipping Hazardous Chemicals off Campus ccccccesssscceceeeeessesseeeceeeeseessnsseaeees 52 5 14 2 Transporting Chemicals on Campus via Purdue Vehicle ccccesssececeeeeeessnseaeees 52 5 14 3 Transporting Chemicals on Campus via Foot cccccesssscceceeecessesscneeeceeeeessesesseaees 54 5 15 Laboratory SOCUPIEY ea ae shasta suds sedensds dies A sdastessonsbeats 55 5 16 Laboratory Self Inspections siini aiaa iaiia eiia 55 5 17 Laboratory Er
156. elect use maintain and decide to retire replace PPE necessary for laboratory work with chemicals Regulatory agency familiarity and compliance topics including OSHA EPA DOT and NRC are also included CHM 605 is open to all departments and is a required course for some departments All graduate students working in a laboratory environment are strongly encouraged to take CHM 605 9 5 REM Researcher s Guide The CHP focuses on work with hazardous chemicals in the laboratory However other common types of hazards are present in many research labs as well e g biological hazards lasers etc REM has developed the Researcher s Guide as a tool to assist researchers with compliance and training requirements for a broad range of common hazards and regulatory programs found in the laboratory Visit the REM Researcher s Guide for more detailed information http www purdue edu rem home files researchers htm 83 The official version of this document will only be maintained online Appendix A CHP Awareness Certification Form Appendix A CHP Awareness Certification Form 84 The official version of this document will only be maintained online Appendix A CHP Awareness Certification Form PURDUE UNIVERSITY Chemical Hygiene Plan and Hazardous Materials Safety Manual Awareness Certification Please Type or Print Legibly For Principal Investigator Building and Room s After reading the Purdue Chemical Hygiene Plan com
157. em injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Alkali Metals Date 9 26 2013 PURDUE UNIVERSITY Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Alkali metal waste should be segregated from all incompatible chemicals such as aqueous solutions Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific alkali metal i e potassium must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from Pl Section 14 Documentation of Training signature of all users is required e Prior to
158. emergency chemical spills or emergency chemical spills 8 1 Non Emergency Chemical Spill Procedures Non emergency chemical spills are generally defined as less than 1 liter do not involve a highly toxic or reactive material do not present a significant fire or environmental hazard and are not in a public area such as a hallway These spills can be cleaned up by properly trained lab personnel using conventional lab PPE e g safety glasses goggles lab coat gloves and the lab spill kit In general when a non emergency spill occurs in the lab the area around the spill should be isolated everyone in the lab should be made aware of the spill and the spilled material should be absorbed and collected using either pads or some other absorbent material such as oil dry or kitty litter Decontamination of the spill area should be conducted using an appropriate solvent soap and water is often the most effective Proper PPE should be worn at all times and only trained personnel should conduct the cleanup Additionally review the SDS s specifically Section 6 Accidental Release Measures to obtain chemical specific cleanup information 8 2 Emergency Chemical Spill Procedures Emergency chemical spills are generally defined as greater than 1 liter involve a highly toxic or reactive compound present an immediate fire or environmental hazard or require additional PPE e g respirator and specialized training to properly cleanup The follow
159. en Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator Piranha Solution Date 3 14 2014 PURDUE UNIVERSITY e An employer requires the use of a respirator e There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Handle with gloves Regular Nitrile gloves do not provide sufficient protection Heavy duty neoprene or rubber gloves must be worn NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with piranha solution Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety goggles are required as well as a face shield Skin and Body Protection Lab coat full length pants and closed toe shoes are required An acid resistant apron is highly recommended Hygiene Measures Avoid contact with skin eyes and clothing Wash hands before breaks and immediately
160. ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Formaldehyde Date 6 18 2013 PURDUE UNIVERSITY Signature Date Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Formaldehyde Date 6 18 2013 PURDUE UNIVERSITY Standard Operating Procedure Hydrofluoric Acid HF This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP
161. ervisor s instructions for PPE which may differ depending on the type and or quantity of flammable combustible liquid being used Use in the smallest practical quantities for the experiment being performed Work must be conducted in a chemical fume hood if air concentrations above 10 of the LEL could be created if the chemical is irritating to the eyes or respiratory system and or is toxic by inhalation Containers should remain closed when not in use This is key to preventing accumulation of flammable vapor concentrations and or accidental ignition Containers should be labeled appropriately Label should indicate the name of the chemical s in the container Avoid using chemical abbreviations acceptable if a legend is present in the lab and formulae When not in use store in flammable storage cabinets if possible Flammable Combustible Liquids Date 7 1 2013 PURDUE UNIVERSITY e Containers should be in good condition and compatible with the material store in safety cans spring closing lid as illustrated to the right if possible Avoid using ignition sources flame burners or any open flame source hot plates electrical equipment with frayed or cracked wiring etc and or creating static electricity in areas where flammable combustible chemicals are being used Ground and bond containers when transferring more than 4 liters of flammable combustible liquids Transport all flammable combustible liquids in secondary containment
162. es flammable storage cabinets that do not contain aqueous or other incompatible chemicals or intrinsically safe refrigerators or freezers that also do not contain aqueous or other incompatible chemicals If pyrophoric materials are received in a specially designed shipping storage or dispensing container such as the Aldrich Sure Seal packaging system ensure that the integrity of that container is maintained Ensure that sufficient protective solvent oil kerosene or inert gas remains in the container while pyrophoric materials are stored Additional Resources e Aldrich Technical Bulletins AL 134 and AL 164 e Handling Pyrophoric Reagents from U S Dept of Energy and Pacific Northwest National Laboratory e Chemical Hygiene and Safety Plan from Berkeley National Laboratory Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Once spilled all liquid or solid pyrophoric chemicals may instantly ignite Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minu
163. es that may occur in the laboratory Safety glasses must be worn at all times by all individuals that are occupying the laboratory area Splash proof safety goggles and or a face shield may be more appropriate depending on the type of work being performed e g transferring hazardous liquids outside of a chemical fume hood or glove box All eye protection equipment must be American National Standards Institute ANSI approved and appropriate for the work being done Eye and face protection may not be required in the lab if the employee is sitting at a workstation or desk that is away from chemical processes e g working at a desktop computer having a lab meeting at a table away from hazardous operations 6 4 5 Hand Protection Each affected employee must wear appropriate hand protection when the hands may be exposed to skin contact of hazardous chemicals cuts abrasions punctures or harmful temperature extremes Chemical resistant gloves must be worn while handling any 64 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy hazardous chemical container regardless of whether the container is open or closed it should be assumed that all chemical containers are contaminated When selecting appropriate gloves it is important to evaluate the effectiveness of the glove type to the specific hazardous chemical being handled Some gloves are more suitable for certain hazardous chemic
164. es the development of RADIATION LASER TYPE WAVELENGTH written SOPs and hazard assessments discussed in scab DORT LTE CLASS 4 LASER PRODUCT detail in Chapter 6 for safe performance ofthe work e Restricting access to areas where hazardous materials are used e Using safety signs or placards to identify hazardous areas designated areas e Labeling all chemicals e Substitution of toxic materials with less toxic materials when possible e Good housekeeping and good personal hygiene such as routine hand washing and regular decontamination of areas that are possibly chemically contaminated such as bench tops and fume hoods e Prohibiting eating and drinking where chemicals are used or stored 4 3 1 Standard Operating Procedures SOPs are written instructions that detail the steps that will be performed during a given procedure and include information about potential hazards and how these hazards will be mitigated SOPs must be prepared by laboratory personnel who are the most knowledgeable and involved with the experimental process However the Laboratory Supervisor is ultimately responsible for approving SOPs regardless of who prepares them The OSHA Lab Standard required SOPs to be developed for all high hazard tasks that are performed in the lab High hazard tasks include any work with the following types of chemicals e Explosives e Water reactive pyrophoric self heating or self reactive chemicals e Particularly hazardous substa
165. esigned to communicate hazards The three most widely used classification systems are the OSHA Globally Harmonized System for Classifying and Labeling Chemicals recently adopted and implemented under the OSHA Hazard Communication Standard the National Fire Protection Association NFPA system of classifying the severity of hazards and the Department of Transportation DOT hazard classes These classification systems are used by chemical manufacturers when creating safety data sheets and chemical labels therefore it is important that Purdue lab employees understand the basic elements of each classification system 2 1 Globally Harmonized System for Classifying Chemicals The Globally Harmonized System GHS is a world wide system adopted by OSHA for standardizing and harmonizing the classification and labeling of chemicals The objectives of the GHS are to e Define health physical and environmental hazards of chemicals e Create classification processes that use available data on chemicals for comparison with the defined hazard criteria numerical hazard classification is based ona 1 5 scale 1 being the most hazardous and 5 being the least hazardous and e Communicate hazard information as well as protective measures on labels and Safety Data Sheet SDS formerly known as Material Safety Data Sheets MSDS 2 1 1 Safety Data Sheets The SDS provides comprehensive information that is imperative for the safe handling of hazardous chemi
166. essing fertilizer manufacturing oil refining wastewater processing and chemical synthesis Section 4 Potential Hazards Highly corrosive chemical Sulfuric acid is harmful if inhaled ingested or absorbed through the skin Inhalation may cause irritation to the respiratory tract with burning pain in the nose and throat coughing wheezing shortness of breath and pulmonary edema Contact with skin causes burns and irritation Eye contact causes burns irritation and may cause blindness Ingestion may cause permanent damage to the digestive tract It is destructive to the tissue of the mucous membranes and upper respiratory tract a et Section 5 Personal Protective Equipment PPE Respirator Protection If Sulfuric acid is being used outside of a chemical fume hood respiratory protection may be required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Respirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean u
167. established by the National Toxicology Program or the International Agency for Research on Cancer regarding the risk of cancer via certain exposure routes It is important to recognize that some substances involved in research laboratories are new compounds and have not been subjected to testing for carcinogenicity 2 Regulated Carcinogens Regulated carcinogens are more hazardous and have extensive additional requirements associated with them The use of these agents may require personal exposure sampling based on usage When working with Regulated Carcinogens it is particularly important to review and effectively apply engineering and administrative safety controls as the regulatory requirements for laboratories that may exceed long term 8 hour or short term 15 minutes threshold values for these chemicals are very extensive B 2 5 2 Reproductive Toxins Reproductive toxins include any chemical that may affect the reproductive capabilities including chromosomal damage mutations and effects on fetuses teratogens Reproductive toxins can affect the reproductive health of both men and women if proper procedures and controls are not used For women exposure to reproductive toxins during pregnancy can cause adverse effects on the fetus these effects include embryolethality death of the fertilized egg embryo or fetus malformations teratogenic effects and postnatal functional defects For men exposure can lead to sterility Examples of em
168. f a chemical fume hood if possible e Ensure that all heat generating equipment has a shut off device installed e Ensure that all water connections on the condenser are clamped securely e Inspect all glassware for defects before setting them up in the experiment e Keep all air and water sensitive drying agents under inert atmosphere Make consistent efforts to not store or use other flammable or hazardous chemicals inside the fume hood where distillation is taking place 47 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 12 5 Laboratory Glassware Broken laboratory glassware is dangerous Glassware related injuries ranging from small cuts to multiple stitches and eye damage are common to lab workers In order to reduce the risk of accidents the following guidelines should be followed Temperature changes can shatter any laboratory glassware Never flash cool glassware with cold water especially after autoclaving or exposure to any high temperatures Only round bottomed or thick walled e g Pyrex evacuated reaction vessels specifically designed for operations at reduced pressure should be used Inspect glassware for any small imperfections before using Sometimes a hairline crack may be present Tap the glassware with a pen and listen to the tone to tell if there is a defect A ringing tone indicates the glassware is fine while a dull thud indicates there is a
169. fety Committee Material Safety Data Sheet National Fire Protection Association Occupational Safety and Health Administration Polychlorinated Biphenyl Particularly Hazardous Substance Principal Investigator Personal Protective Equipment Resource Conservation and Recovery Act Recombinant Deoxyribonucleic Acid Radiological and Environmental Management Radiation Safety Committee Satellite Accumulation Area Safety Data Sheet Standard Operating Procedure Upper Explosive limit 1 The official version of this document will only be maintained online Chapter 1 Introduction Chapter 1 Introduction Laboratory safety is an integral part of laboratory research and is essential to ensure that Purdue University s compliance with all applicable environmental health and safety laws regulations and requirements are met The risks associated with laboratory research workplace injuries environmental incidents and property losses or damage are greatly reduced or eliminated when proper precautions and practices are observed in the laboratory To better manage and mitigate these risks Purdue University has developed the Chemical Hygiene Plan CHP which is intended to be the cornerstone of your laboratory safety program and is designed to aid faculty staff and students in maintaining a safe environment in which to teach and conduct research Each laboratory using hazardous materials is required to have a copy of the CHP readily available to
170. filters need to be changed ensure the maintenance is performed by a qualified individual 30 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e Use required PPE as instructed by the PPE Policy detailed in Chapter 6 5 2 Housekeeping Housekeeping is an important element to a laboratory safety program A clean well maintained lab improves safety by preventing accidents and can enhance the overall efficiency of the work being performed The following laboratory housekeeping guidelines should be followed e All doorways and hallways must be free of obstructions to allow clear visibility and exit The laboratory should be uncluttered without excessive storage of materials that could cause or support a fire e g paper cardboard flammable liquids etc e Fire protection sprinklers must be unobstructed a minimum of 18 inches of clearance is required below the sprinkler head If the laboratory does not have fire protection sprinklers there must be a minimum of 24 inches of clearance below the ceiling e Do not store items that block fire extinguishers or eyewash and safety shower stations e Donot store items in front of electrical boxes panels in the lab e Aroutine cleaning schedule should be established All work surfaces should be kept as clean as possible All potentially chemically contaminated work area surfaces e g chemical fume hood deck countertops should be cleaned r
171. for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for Potassium Cyanide must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with potassium cyanide designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Potassium Cyanide Date 11 26 2013 PURDUE
172. formaldehyde is reported to be as low as 0 1 ppm Exposure Limits OSHA PEL 8 HR TWA OSHA Short Term Exposure Limit ACGIH TLV TWA DOO Section 5 Personal Protective Equipment PPE Respirator Protection If formaldehyde is being used outside of a chemical fume hood e g during specimen preservation respiratory protection is likely required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Respirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL As PPE in the event of a chemical spill clean up process Formaldehyde Date 6 18 2013 PURDUE UNIVERSITY Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended Wearing two pairs of nitrile gloves is recommended If handling procedures require the
173. formation Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical Section 3 Physical Chemical Properties Physical Chemical Properties CAS 71 43 2 CI Hazardous Class GHS Classification Flammable Liquid Carcinogen Target Organ Effect Irritant Mutagen Molecular Formula CH6 Form physical state Liquid Color Colorless Benzene Date 8 19 2013 PURDUE UNIVERSITY Boiling Point 80 C Flash Point 11 C Lower Explosive Limit 1 3 V Upper Explosive Limit 8 V Section 4 Potential Hazards Benzene is an OSHA regulated carcinogen It is a highly flammable liquid and may form explosive mixtures with air Harmful if inhaled causes respiratory tract irritation Harmful if absorbed through skin Causes eye and skin irritation May cause cancer and genetic defects Exposure Limits OSHA PEL 8 HR TWA 0 5 ppm OSHA Short Term Exposure Limit 1 ppm ACGIH TLV TWA 0 1 ppm DOO Section 5 Personal Protective Equipment PPE Respir
174. from an electrical plug is prohibited Flexible cords may only be plugged into grounded receptacles Adapters that interrupt the continuity of the equipment grounding connection may not be used All portable electric equipment and flexible cords used in highly conductive work locations such as those with water or other conductive liquids or in places where employees are likely to contact water or conductive liquids must be approved for those locations Employee s hands must be dry when plugging and unplugging flexible cords and cord and plug connected equipment if energized equipment is involved 57 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e Ifthe connection could provide a conducting path to the employee s hands e g if a cord connector is wet from being immersed in water the energized plug and receptacle connections must be handled only with insulating protective equipment e Lamps for general illumination must be protected from breakage and metal shell sockets must be grounded e Temporary lights must not be suspended by their cords unless they have been designed for this purpose e Extension cords are considered to be temporary wiring and must also comply with the section on Requirements for Temporary Wiring in this program 5 18 3 Temporary Wiring Requirements Temporary electrical power and lighting installations 600 volts or less including flexible
175. ful exposure due to an atmospheric contaminant in the absence of PEL As PPE in the event of a chemical spill clean up process Nitric Acid Date 7 2 2013 PURDUE UNIVERSITY Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Handle with gloves Viton butyl butyl rubber or neoprene gloves are recommended for handling concentrated nitric acid gt 70 Latex and nitrile gloves are not recommended for handling concentrated nitric acid NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with nitric acid Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or goggles are required A face shield is also recommended Skin and Body Protection Lab coat full length pants and closed toe shoes are required Hygiene Measures Avoid contact with skin eyes and clothing Wash hands before breaks and immediately after handling the product Section 6 Engineering Controls Use of nitric acid should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be
176. g compressed gas that may be associated with working in the glove box e All equipment and chemicals in the glove box must be organized and all chemicals must be labeled Do not allow items particularly chemicals to accumulate in the glove box 4 2 3 Laminar Flow Clean Benches A laminar flow clean bench as shown in Figure 4 4 is an enclosed bench designed to prevent contamination of semiconductor wafers samples or any particle sensitive device Air is drawn through a filter and blown in a very smooth laminar flow towards the user Therefore it is critical that absolutely no hazardous chemicals infectious and or radioactive materials ever be used in a laminar flow clean bench as the vapors are blown directly towards the user Applications that involve the use of chemicals should be conducted in chemical fume hoods 4 2 4 Biological Safety Cabinets A biological or biosafety safety cabinet as shown in Figure 4 5 is an enclosed ventilated laboratory workspace for safely working with materials contaminated with or potentially contaminated with infectious materials The primary purpose of a biosafety cabinet is to serve as a means to protect the laboratory worker and the surrounding environment from 23 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls pathogens All exhaust air is filtered as it exits the biosafety cabinet removing harmful particles Biological safety
177. gOnOmies neea rer E a a ia A EEEN at 56 5 18 Laboratory Electrical Safety c ccccccccccsssssssscececececsessnseseeeceescsesesseaeseeeeseeseessaeaeeeesesseeeea 56 5 18 11 Training ser en nina and 56 5 18 2 Portable Electrical Equipment and Extension Cords uuseenerseneennnnnsnennnnnnennennnnennnnnn 56 5 18 3 Temporary Wiring Requirements eeesesessesnsennensensnnennnnnnnennnnnennnnnsnnnennnnnennn nennen 58 5 18 4 Wet or Damp Locations ereeesssssnonssssenenenennnnnnnnennnnnnnnnnnnennennnnnnnnnnnnensnnnennnnn nn nn 59 Chapter 6 Laboratory PPE Poliev nah 60 6 1 PURDOSC A ner ENTE Re E 60 ii The official version of this document will only be maintained online Table of Contents Ord SCOP OG ern NR ENT cetsaieisdbeneases e T 60 6 3 4 Hazard Assessment ereignete 60 6 3 1 Task Evaluation Hazard Assessment sense 61 6 3 2 Location Evaluation Hazard ASSCSSMENL sseccececeenececsesaeeecesseeeeceesenaeeeseeaaeeeenes 61 6 3 3 Job Title Evaluation Hazard Assessment eeesssseessssnneessnnnnnennennnnnnnnnnnnnnnnnnnnnnnnnnnnnn 62 6 4 Minimum PPE Requirements for Laboratories uusueneneesennsnnnsnenennnnnnnennnnnnnnnnnnnennennnnnn 62 6 4 1 Head Proteetion a n nee 63 6 4 2 Hearing Protection essn sn ee nee ern hats Segeln rinnen 63 6 4 3 Respiratory Protectio Mi ateina aa a ei Eaa Taaa 64 6 4 4 Eyeand Face Protection s 2s f isceie ei astivcbesheeessscss a a E a teas taweeeds
178. ge cabinet that does not contain incompatibles Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Formaldehyde Date 6 18 2013 PURDUE UNIVERSITY Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 t
179. gen Reproductive Toxins or Extremely Toxic Chemicals label provided by REM as shown to the right Avoid contact with skin and eyes and inhalation Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such as oxidizers Containers which are opened must be carefully resealed and kept upright to prevent leakage A suitable storage location is a flammable storage cabinet or lab cabinet that does not contain incompatibles Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threateni
180. gure 7 7 Properly Labeled Unknown Waste 7 6 2 Identifying Unknown Chemicals Every effort should be made by laboratory personnel to identify unknown chemicals Here are a few steps that can be taken to help this effort 75 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 1 Ask other laboratory personnel if they are responsible for or can help identify the unknown chemical 2 The type of research conducted in the laboratory can be useful information for making this determination Eliminating certain chemicals as a possibility helps narrow the problem as well This is especially important for mercury PCB or dioxin compounds because they must be managed separately from other hazardous waste 3 For trade products contact the manufacturer or search online to obtain an SDS REM staff can assist you in finding an SDS 7 6 3 Removing Unknown Chemicals from the Work Area If it is not possible to identify the material a Hazardous Waste label should be placed on the container as described above in Section 7 6 1 and a Hazardous Materials Pickup Request Form should be submitted which describes all of the available information e g 4 liter container of clear liquid Call REM at 765 49 40121 if you have a question about an unknown 7 6 4 Preventing Unknown Chemicals Here are a few tips that will help prevent the generation of unknown chemicals e Label all chemical container
181. h a temperature and pressure and at such a speed that can cause damage to the surroundings Pyrotechnic substances are included even when they do not evolve gases A pyrotechnic substance or mixture is designed to produce an effect by heat light sound gas or smoke or a combination of these as the result of non detonative self sustaining exothermic chemical reactions An explosive compound that is sometimes found in a laboratory setting is picric acid 2 4 6 trinitrophenol If a laboratory plans to work with explosive compounds contact REM for further instructions before any work occurs B 2 Health Hazards A chemical is a health hazard if there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed employees Each health hazard is defined and briefly discussed below B 2 1 Irritants Irritants are defined as chemicals that cause reversible inflammatory effects on living tissue by chemical action at the site of contact A wide variety of organic and inorganic compounds including many chemicals that are ina powder or crystalline form are irritants Symptoms of exposure can include reddening or discomfort of the skin and irritation to respiratory systems B 2 2 Sensitizers A sensitizer allergen is a substance that causes exposed individuals to develop an allergic reaction in normal tissue after re
182. h as polyethylene or other non reactive acid solvent bottle carrier Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Sulfuric acid Date 3 19 2014 PURDUE UNIVERSITY Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Sulfuric acid waste cannot be disposed of down the drain When possible do not mix acidic and basic waste streams Make sure the waste container s is properl
183. hands to be submerged in Formaldehyde solution then disposable gloves are not suitable Check the resources below for a more suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Formaldehyde Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of Formaldehyde must be conducted in a properly functioning chemical fume hood The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute
184. he SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Ethidium Bromide Date 7 19 2013 PURDUE UNIVERSITY Standard Operating Procedure Flammable and Combustible Liquids This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number
185. here anyone can readily have access to them These types of materials should be stored in a secure location and lab personnel should always be present when these materials are in use 5 16 Laboratory Self Inspections REM performs laboratory inspections for various purposes e g routine building safety and compliance inspections However the Laboratory Supervisor a qualified designee should also inspect the laboratory for compliance with the requirements of the CHP at a minimum on an annual basis Lab personnel have a much greater understanding of the unique hazards and issues that are encountered in their individual lab than REM does The goal of these inspections is to identify and correct unsafe and non compliant conditions that could potentially result in an injury to lab personnel or a fine from a regulatory agency e g open hazardous waste container All deficiencies found during the inspection should be reviewed and corrected The following elements should be performed during these inspections e Housekeeping practices should be reviewed Chemicals should be stored appropriately and labeled Evidence of spills and or chemical contamination should be cleaned All glassware and equipment should be stored appropriately etc e Hazard assessments should be updated if process changes have occurred For example the lab is now performing organic synthesis and working with organometallic compounds e Training records should be updated and docu
186. ical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Benzene Date 8 19 2013 PURDUE UNIVERSITY Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for Benzene must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS
187. ich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additional lab protocol may be added here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with liquid nitrogen designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Liquid Nitrogen Date 5 9 2014 PURDUE UNIVERSITY Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Liquid Nitrogen Date 5 9 2014 PURDUE UNIVERSITY Standard Operating Procedure Lithium Alkyl Compounds This is an SOP template and is not complete until 1 lab specific
188. id any skin contact Nitrile gloves are recommended Check the resources below for the most suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific oxidizing liquids or solids being used Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required A face shield may also be appropriate depending on the specific application Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Rinse immediately contaminated clothing and skin with plenty of water before removing clothes Section 6 Engineering Controls Use of oxidizing liquids and solids should be conducted in a properly functi
189. ilable to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with HF designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Hydrofluoric Acid HF Date 2 1 2013 PURDUE UNI
190. information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone 765 494 4573 Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this SOP Building Room Number Section 2 Type of SOP C Process Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A GHS Classification Pyrophoric Water Reactive Flammable Liquid Corrosive Molecular Formula N A aoro sA Butyliithium solution 2 oo Lows 3 Bufyifithjum Teper Form physical state solid or liquid Color N A Lithium alkyls Date 2 1 2013 PURDUE UNIVERSITY Boiling point N A Uses Lithium alkyl compounds are pyrophoric chemicals that spontaneously ignite on contact with air These compounds react violently with water and vapors may form explosive mixture with air Lithium alkyl compounds are widely u
191. ing procedures should be followed in the event of an emergency chemical spill e Cease all activities and immediately evacuate the affected area make sure that all personnel in the area are aware of the spill and also evacuate e If chemical exposure has occurred to the skin or eyes the affected personnel should be taken to the nearest safety shower and eyewash station 79 The official version of this document will only be maintained online Chapter 8 Chemical Spills e Dial 911 which will initiate both the Purdue Police and Purdue Fire Department response if the situation is or could become an emergency e g chemical exposure has occurred a fire or explosion has occurred e The fire alarm should be pulled which will initiate building evacuation if any of the following occurs o A fire and or explosion has occurred or there is a threat of fire and or explosion o The large spill which is either highly toxic or presents an immediate fire or environmental hazard is in a public area such as a hallway o Toxic vapors are leaving the area where the spill has occurred such as seeping from the laboratory into the hallway or neighboring rooms o You are unsure of the hazards and feel that the spill could be harmful to building occupants o Ensure that no one else is allowed to enter the area until the spill has been properly cleaned up by the Purdue Fire Department 8 3 Chemical Spill Kits Each laboratory should have a
192. ing to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile and latex disposable gloves are NOT suitable Polyvinyl Acetate Viton or fluorinated rubber gloves are recommended Check the resources below for a more suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Methylene chloride Methylene Chloride Date 9 26 2013 PURDUE UNIVERSITY Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Face shield is also recommended ifthere is a high probability of a splash hazard Skin and Body Protection Lab coats 100 cotton must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel must also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are oc
193. ion http www purdue edu rem hmm timesensitive htm Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended Wearing two pairs of nitrile gloves is recommended NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with diethyl ether Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf Diethyl Ether Date 8 19 2013 PURDUE UNIVERSITY OR http www showabestglove com site default aspx OR http www mapaglove com E
194. ion 14 Documentation of Training signature of all users is required e Prior to conducting any work with base baths designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Base Baths Date 1 17 2014 PURDUE UNIVERSITY Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Base Baths Date 1 17 2014 PURDUE UNIVERSITY Standard Operating Procedure Benzene This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific In
195. ion 8 Special Handling and Storage Requirements e Precautions for safe handling Pyrophoric use extreme care when handling e Only handle under inert gas use a glove box if possible Do not expose to air e Avoid contact with skin and eyes and inhalation Pyrophoric Materials PURDUE UNIVERSITY e A dry run of the experiment should be performed using low hazard materials such as water or an organic solvent Never work with pyrophoric materials alone Conduct the procedure only after a supervisor has observed the user performing the proper technique unassisted All glassware used for pyrophoric materials should be oven dried and free of moisture Keep away from sources of ignition Avoid heat and shock or friction when handling Secure all pyrophoric material containers to a stand Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances The amount of pyrophoric materials stored should be kept at a minimum Any expired or unnecessary reactive materials should be properly disposed of as hazardous waste All pyrophoric materials should be clearly labeled with the original manufacturer s label which should have the chemical name hazard labels and pictograms The label should not be defaced in any way All pyrophoric material should be placed into secondary containment as a precautionary measure Suitable storage locations include inert gas filled desiccators or glove box
196. is a strong oxidizer It is corrosive and can cause severe burns to the skin and eyes It is extremely destructive to the tissue of the mucous membranes and upper respiratory tract It may be harmful if inhaled ingested or absorbed through the skin Symptoms of exposure include burning sensation coughing wheezing laryngitis shortness of breath headache nausea vomiting cyanosis cardiovascular effects respiratory disorders lachrymation nose bleeding vertigo irritability loss of appetite joint pain abdominal pain diarrhea ulceration dizziness pulmonary edema hoarseness and permanent corneal opacification Prolonged exposure may cause lung damage Bromine has a permissible exposure limit PEL of 0 7 mg m Section 5 Personal Protective Equipment PPE Respirator Protection If Bromine is being used outside of a chemical fume hood respiratory protection may be required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Respirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contamin
197. ition source Boiling Point The temperature at which the vapor pressure of a liquid equals the atmospheric pressure and the liquid changes into a vapor Auto Ignition Temperature The minimum temperature at which self sustained combustion will ee occur in the absence of an ignition source 100 AIR Lower Explosive Limit LEL The lowest concentration percentage of a gas or a vapor in air capable of producing a flash of fire in presence of an ignition source arc flame heat Upper Explosive Limit UEL Highest concentration percentage of a gas or a vapor in air capable of producing a flash of fire in presence of an ignition source arc flame heat Section 4 Potential Hazards Flammable liquid toxic by inhalation harmful by skin absorption corrosive water reactive reacts violently with water forming hydrogen chloride an extremely toxic corrosive gas CH3CI3Si H20 CH Si OH 3 HCl GLYN ZED Section 5 Personal Protective Equipment PPE Respirator Protection Methyltrichlorosilane may only be used in a properly functioning chemical fume hood Respirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a res
198. l Hazards Corrosive May be harmful if inhaled ingested or absorbed through the skin Inhalation may cause irritation to the respiratory tract with burning pain in the nose and throat coughing wheezing shortness of breath and pulmonary edema Contact with skin causes burns and irritation Eye contact causes burns irritation a may cause blindness Ingestion may cause permanent damage to the digestive tract It is destructive to the tissue of the mucous membranes and upper respiratory tract Acids and bases may have other hazards associated with them such as flammability oxidizer or toxicity Note Refer to SOP of Hydrofluoric acid HF for specific hazards and safety information a dt Section 5 Personal Protective Equipment PPE Respirator Protection If corrosives are being used outside of a chemical fume hood respiratory protection may be required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Respirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted Corrosives Date 10 1 2013 PURDUE UNIVERSITY e When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due
199. l of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Diethyl Ether Date 8 19 2013 PURDUE UNIVERSITY Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Diethyl ether waste should be segregated from all incompatible chemicals such as oxidizers Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the diethyl ether SDS must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with diethyl ether designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamin
200. lass Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CASH Sulfuric acid 7664 93 9 Hydrogen peroxide 7722 84 1 GHS Classification Corrosive Oxidizer Molecular Formula H2SO and HO Form physical state Liquid Color Colorless Piranha Solution Date 3 14 2014 PURDUE UNIVERSITY Uses Piranha solution also known as piranha etch is a mixture of Sulfuric acid H2504 and Hydrogen peroxide H202 used to clean organic residues off substrates Because the mixture is a strong oxidizer it will remove most organic matter and it will also hydroxylate most surfaces add OH groups making them extremely hydrophilic water compatible Piranha solution is used frequently in the microelectronics industry e g to clean photoresist residue from silicon wafers The traditional piranha solution is a 3 1 mixture of Sulfuric acid and 30 Hydrogen peroxide The solution may be mixed before application or directly applied to the material applying the Sulfuric acid first followed by the peroxide Piranha solutions are extremely energetic and may result in explosion or skin burns if not handled with extreme caution Section 4 Potential Hazards Piranha solution is very dangerous being both strongly acidic and a strong oxidizer Both liquid and vapour forms are extremely corrosive to skin and respiratory tract Direct contact will create skin burns and will be extremely destructive to mucous me
201. ls vials etc must be labeled If is not practical to label a secondary container that is in process e g reaction vessel flask a temporary label can be used as shown in Section 5 7 of the CHP Reaction vessels in chemical fume hoods must be labeled as well If labeling the vessel itself is not practical the hood sash or wall may be labeled as illustrated in Figure 4 2 f 4 Figure 4 2 Alternative Labeling of Chemical Fume Hood Reaction Vessels Do not allow the vents or air flow baffles to be blocked Never put your head inside of an operating chemical fume hood All chemical fume hoods should be routinely checked for airflow by measuring the face velocity which should be between 70 125 feet per minute REM conducts face velocity readings on a routine basis and records this information on the hood label Contact REM with questions regarding chemical fume hoods 765 49 46371 21 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls 4 2 2 Glove Boxes A glove box as illustrated in Figure 4 3 is a sealed container that is designed to allow one to handle material in a defined atmosphere typically inert Glove boxes can be used to protect sensitive items inside or the user on the outside or both The following recommendations should be followed by all personnel using a glove box Figure 4 3 Glove Box e All personnel must receive documented training fro
202. m Laboratory Supervisors of all health and safety requirements and assist with the selection of appropriate safety controls engineering controls administrative controls and PPE e Ensure that Laboratory Supervisors have the necessary resources to maintain compliance with the CHP and that all lab staff receive appropriate training e Actas the liaison between the Laboratory Supervisors and the Chemical Laboratory Safety Committee e Conduct periodic lab inspections and immediately take steps to abate hazards that may pose a risk to life or safety upon discovery of such hazards e Ensure that SOPs and hazard assessments are being prepared e Maintain employee exposure monitoring records when applicable e Help to develop and implement appropriate environmental health and safety policies and procedures e Review and evaluate the effectiveness of the CHP program at least annually and update it as appropriate and e Actively enforce all applicable safety procedures and ensure the contents of the CHP are followed take appropriate actions when safety procedures are not followed 1 5 Radiological amp Environmental Management Department The Radiological and Environmental Management Department REM serves as the environmental health and safety department for Purdue University REM s primary role is to manage regulatory compliance with all federal state and Purdue regulations involving environmental health and safety issues REM facilitates
203. m the PI or delegate before any work in a glove box occurs All trained personnel must understand the design features and limitations of a glove box before use The training must include detailed instruction on elements such as the ventilation and vacuum controls that maintain a pressure differential between the glove box and outside atmosphere atmospheric controls e g controlling oxygen concentrations and moisture etc e Prior to use a visual glove inspection must be performed Changing of a glove must be documented date manufacturer model of glove and person performing change Gloves should not be used until they fail they should be changed according to the glove box manufacturer s recommendations or whenever necessary e Plugging ports that are never or infrequently used is recommended A properly plugged port should have a stub glove and a glove port cap installed e Chemical resistant gloves e g disposable nitrile gloves should be used under the glove box gloves to protect from contamination e The glove box pressure must be checked every day before use and immediately after gloves are changed The pressure check must be documented 22 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls e Keep sharps in an approved container while in the glove box e Do not work in the glove box unless the lighting is working e Follow all safe work practices for using and handlin
204. mbranes upper respiratory tract and eyes The Piranha solution is very energetic exothermic and potentially explosive Mixing the solution is exothermic The resultant heat can bring solution temperatures up to 120 C Handle with care When preparing the Piranha solution always add the peroxide to the acid One must allow the solution to cool reasonably before applying any heat The sudden increase in temperature can also lead to violent boiling or even splashing of the extremely acidic solution Also explosions may occur if the peroxide solution concentration is more than 50 30 peroxide in water solution is more reasonable Piranha solution reacts violently with any organic materials Avoid mixing with incompatible materials such as acids bases organic solvents acetone isopropyl alcohol or nylon Only use clean glass or Pyrex containers Piranha solutions are not compatible with plastic Potential Health Effects Inhalation Harmful if inhaled Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract Skin Harmful if absorbed through skin Causes skin burns Eyes Causes severe eye burns Ingestion Harmful if swallowed amp Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted Wh
205. mented if new lab personnel have not yet been trained or if any processes have changed e Excess or outdated chemicals should be properly disposed of by REM 55 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e Safety supplies such as PPE and spill containment equipment should be replenished if necessary A recommended template to use during laboratory self inspections is the ISP Self Audit Checklist which can be found on the REM website https www purdue edu rem home forms ispcheck pdf 5 17 Laboratory Ergonomics Many tasks in laboratories require repetitive motions which may lead to cumulative trauma injuries of the body these effects can be long term Tasks like pipetting weighing multiple samples standing at the bench or hood and using microscopes for long periods of time can cause physical stress Even time compiling data at a computer poses potential physical problems Ergonomics is the study of interaction of the human body with the work environment Ergonomics strives to fit the job to the body through proper body positioning posture movement tools workplace layout and design Parts of the body commonly affected by poor ergonomics include neck shoulders back hands wrists elbows legs and feet REM has resources available to improve ergonomic conditions and help reduce cumulative trauma injuries to laboratory workers Often simple adjustments are all that i
206. ments e Do not over purchase only purchase what can be safely stored in the laboratory Avoid contact with skin eyes and clothing Avoid inhalation of vapor or mist Avoid formation of dust Always use inside a chemical fume hood Use spark proof tools and explosion proof equipment Keep away from heat and sources of ignition No smoking Prevent the build up of electrostatic charge Handle bromine only with equipment made of Kynar Teflon Monel Pyrex glass or lead lined steel Keep container upright amp tightly closed in a dry and well ventilated place Containers which are opened must be carefully resealed and kept upright to prevent leakage Keep away from incompatible materials such as reducing agents alkali metals powdered metals aluminum stainless steel iron copper organic materials aldehydes ketones arsenic powder amines amides phenols alcohol ammonia azides and ozone Bromine will attack some types of plastics rubber and coatings Use in the smallest practical quantities for the experiment being performed Containers should remain closed when not in use Do not store in polyethylene containers Containers should be labeled appropriately Label should indicate the name of the chemical s in the container Avoid using chemical abbreviations acceptable if a legend is present in the lab and formulae Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware
207. minimum temperature at which self sustained combustion of a substance will occur upon or after exposure to an ignition source GE Boiling Point The temperature at which the vapor pressure of a liquid equals the atmospheric 0 AIR pressure and the liquid changes into a vapor Auto Ignition Temperature The minimum temperature at which self sustained combustion will occur in the absence of an ignition source Lower Explosive Limit LEL The lowest concentration percentage of a gas or a vapor in air capable of producing a flash of fire in presence of an ignition source arc flame heat Upper Explosive Limit UEL Highest concentration percentage of a gas or a vapor in air capable of producing a flash of fire in presence of an ignition source arc flame heat 0 GAS 100 AIR Section 4 Potential Hazards Flammable or combustible liquid Keep away from heat sparks open flames hot surfaces Flammable and combustible liquids often have other hazards associated with them such as toxicity and the ability to form explosive organic peroxides Make sure that all of the potential hazards are understood before handling any chemical Section 5 Personal Protective Equipment PPE Respirator Protection If flammable and or combustible liquids are being used outside of a chemical fume hood respiratory protection may be required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Respi
208. mployee is retrained 6 7 Injuries Illnesses and Medical Examinations Employees must notify their Laboratory Supervisor of all injuries and illnesses regardless of how the magnitude The laboratory supervisor must ensure that a First Report of Injury form is completed Employees should report to a Purdue approved occupational medical provider http www purdue edu hr Benefits wcimmediatecare html treatmentFacilities if medical attention is required Note The Purdue University Student Hospital is not an approved occupational medical provider If the injury is serious and presents an emergency situation dial 911 and emergency responders Purdue Fire Department if located on the West Lafayette Campus will respond and transport the patient to a local hospital emergency room For more information regarding the First Report of Injury reporting process visit the REM webpage http www purdue edu rem injury froi htm Departments must provide all employees who work with hazardous chemicals an opportunity to receive medical attention including any follow up examinations which the examining physician determines to be necessary under the following circumstances e Whenever an employee develops signs or symptoms associated with a hazardous chemical to which the employee may have been exposed in the laboratory e Where exposure monitoring reveals an exposure level routinely above the action level or in the absence of an action level the permissi
209. n 1 liter of flammable liquids flammable solids corrosives oxidizers or organic peroxides at one time e High hazard tasks can also include work with equipment that creates particularly hazardous conditions Examples include solvent distillation work with high pressure systems hydrogenation work with cryogenic chemicals such as liquid nitrogen etc 82 The official version of this document will only be maintained online Chapter 9 Training Laboratory Supervisors must ensure that all applicable personnel receive documented training on lab specific SOPs More information regarding SOPs can be found on the REM website http www purdue edu rem home files sop htm 9 4 Laboratory Chemical Safety Course CHM 605 The Purdue Department of Chemistry offers a graduate level zero credit fall semester course CHM 605 that covers the fundamentals of laboratory chemical safety practices CHM 605 includes a strong focus on learning how to find read interpret and use the information in safety data sheets chemical labels and other printed chemical safety information Other topics covered include fire protection prevention electrical safety laser and ionizing radiation safety machine safety pumps autoclaves centrifuges and non PPE safety equipment hoods extinguishers fire protection systems and building elements general ventilation showers eyewashes and an understanding of administrative controls engineering controls and how to s
210. n Life Threatening Emergency Ethidium Bromide Date 7 19 2013 PURDUE UNIVERSITY Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Collect Waste Ethidium bromide waste must be collected and managed as hazardous waste not biological waste PPE absorbent materials liners or other debris contaminated with ethidium bromide must also be collected as hazardous waste All solid ethidium bromide waste including debris and dry agarose gels should be placed in an appropriate container and labeled accordingly Please double bag your solid waste in two black trash bags Do not use orange biohazard waste bags unless the waste is also infectious The buffers and solutions used with ethidium bromide gels can be submitted to REM as chemical waste or put down the drain only if a filter especially designed for removal of ethidium bromide is used There are several commercially available filtering systems that can be purchased One such product can be found at the following Extractor EtBr Waste Reduction System Contact REM at 49 40121 for further details Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers a
211. n at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Rinse immediately contaminated clothing and skin with plenty of water before removing clothes Section 6 Engineering Controls Use of Sulfuric acid should be conducted in a properly functioning chemical fume hood whenever possible Avoid inhalation of vapor or mist The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention Sulfuric acid Date 3 19 2014 PURDUE UNIVERSITY If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Section
212. n the laboratory e All samples must be kept closed except when in use Storage in beakers or flasks should be temporary If temporarily storing samples in beakers or flasks a u Na h a cork Parafilm or some other closure device must oT be used e All samples must be labeled with the chemical name date the sample was developed received and the name of generator Chemical structure or a labeling system that is only known to lab personnel is not acceptable as the only means of labeling samples Acronyms can be used as a labeling system as long as an up to date legend is posted in the lab e Samples should be disposed of within 6 months unless actively being used for analysis Stockpiling unusable samples in not an acceptable practice All samples that are no longer necessary must be properly disposed of in a timely manner using REM s hazardous waste program e Samples must be stored according to the primary hazard class this should be done to the best of your ability considering the properties that are known or assumed such as toxicity e lf the hazard s of a sample are unknown the Laboratory Supervisor must attempt to determine whether it is hazardous or not Assume all samples are toxic unless otherwise 50 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan demonstrated This can be accomplished by literature review or reviewing the hazards of other similar
213. nces which includes carcinogens reproductive toxins and acutely toxic substances e Compressed gases 26 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls e Work involving more than 1 liter of flammable liquids flammable solids corrosives oxidizers or organic peroxides at one time e High hazard tasks can also include work with equipment that creates particularly hazardous conditions Examples include solvent distillation work with high pressure systems hydrogenation work with cryogenic chemicals such as liquid nitrogen etc REM develops SOP templates that can be used by laboratories These SOPs are not complete as is they are templates that must be customized by each laboratory before they are considered complete Instructions for completion are included in each SOP template Laboratories are encouraged to use this template format to develop their own SOPs Contact REM at 765 49 40121 if assistance is needed with developing lab specific SOPs For the up to date list of SOP templates visit the REM website http www purdue edu rem home files sop htm 4 3 2 Required Laboratory Postings The following forms and labels are required to be posted in most campus laboratories e The Emergency Contact Door Posting is required for all laboratories and can be found on the REM webpage https www purdue edu rem home forms doorpost pdf e The Certification of Hazard Assessment F
214. ncipal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 75 79 6 GHS Classification Flammable Liquid Acute Toxicity oral inhalation dermal Skin Corrosion Serious Eye Damage Specific Target Organ Toxicity Molecular Formula CH3CI3Si Form physical state Liquid Color N A Methyltrichlorosilane Date 12 13 2013 PURDUE UNIVERSITY Boiling Point 66 C Flash Point 8 C Auto ignition temperature gt 404 C not pyrophoric Lower Explosive Limit 7 2 V Upper Explosive Limit 11 9 V Relative Vapor Density 5 16 air 1 0 Density 1 273 g cm at 25 C Important Definitions Flammable Liquid Liquids having a flash point below 38 C 100 F Te Combustible Liquid Liquids having a flash point at or above 38 C 100 F and no greater than 0 AIR 93 C 200 F Flash Point The minimum temperature at which vapors are formed on the surface of a substance in sufficient quantity to ignite when exposed to an ign
215. nd does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Pyrophoric waste should be segregated from all incompatible chemicals such as aqueous solutions Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage f
216. nd in a designated area All ethidium bromide waste containers must be suitable for transportation and must not be leaking Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific ethidium bromide powder or solution and concentration must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required Ethidium Bromide Date 7 19 2013 PURDUE UNIVERSITY e Prior to conducting any work with ethidium bromide designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of t
217. neered container and or cap designed for LC waste collection Figure 7 9 shows several examples of acceptable solutions for proper LC waste collection that can be purchased Safety Cans Ported Cap No Threads 4 Port threaded Cap Solvent Bottle Cap Figure 7 9 Proper LC Waste Collection Options for Purchase 2 An existing cap can be modified by the research lab for LC waste collection To modify an existing cap a hole can be drilled into a cap The diameter of the hole should be similar to the diameter of the waste line there should be a tight fit between the container opening and waste line In addition a hole should be drilled to accommodate any exhaust filter or air valve tube that may be required It is recommended that either a 4 liter container or 5 gallon carboy be used for waste collection The modified cap should be replaced with a regular unmodified cap once the container is full and ready for REM pickup See Figure 7 10 for examples of acceptable modified caps 78 The official version of this document will only be maintained online Chapter 8 Chemical Spills Chapter 8 Chemical Spills Chemical spills in the laboratory can pose a significant risk to human health and the environment All lab personnel must be trained on how to properly respond to chemical spills in order to minimize risk In general chemical spills can be placed into one of two categories non
218. nerator of the waste wastes should remain in the same room they were generated in A central waste collection room should not be established e The waste storage volume should never exceed 55 gallons per SAA e Containers should be segregated by chemical compatibility during storage e g acids away from bases secondary containment can be used as a means of segregation e Avoid halogenated and non halogenated wastes in the same waste container e Avoid mixing incompatible waste streams in the same container e g acids with bases oxidizers with organic solvents that will potentially create an exothermic reaction in the waste container If mixing waste streams does create heat allow the container to vent and cool in a chemical fume hood before sealing to avoid over pressurization of the container as illustrated in Figure 7 2 e Collect all highly toxic reactive mercury and any exotic wastes e g dioxin compounds PCBs controlled substances separately even if they are chemically compatible with other waste streams Failing to do so can result in costly disposal fees e g mixing mercury with an organic solvent waste means that the entire waste stream must be treated as mercury waste e All spills and leaks should be cleaned up immediately e Identification of SAAs is not required by the EPA but it is recommended as a good practice 71 The official version of this document will only be maintained online Chapter 7 Hazardous Wast
219. ng Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet or lab cabinet is recommended Methylene Chloride Date 9 26 2013 PURDUE UNIVERSITY Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for Methylene chloride must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all user
220. ng Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical CI Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 50 00 0 GHS Classification Combustible Liquid Target Organ Effect Toxic by Inhalation Toxic by Ingestion Toxic by Skin Absorption Skin Sensitizer Corrosive Carcinogen Molecular Formula CH O Form physical state Liquid Color Clear Formaldehyde Date 6 18 2013 PURDUE UNIVERSITY Boiling Point N A Flash Point 64 C 147 F Lower Explosive Limit 7 V Upper Explosive Limit 73 V Relative Vapor Density 1 04 Air 1 0 Uses Formaldehyde also formalin is an OSHA regulated carcinogen commonly used as fixative for tissues in various biological applications Section 4 Potential Hazards Formaldehyde is classified by OSHA as a combustible liquid target organ effect toxic by inhalation toxic by ingestion toxic by skin absorption skin sensitizer corrosive and carcinogen Do not breathe dust fumes gas vapor mist or spray Toxic if inhaled Material is extremely destructive to the tissues of the mucous membranes and upper respiratory tract Causes eye burns Approximately 1 5 grams of vaporized Formaldehyde will achieve this concentration in a typical laboratory setting The odor threshold of
221. ng Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Lithium alkyls Date 2 1 2013 PURDUE UNIVERSITY Standard Operating Procedure Methylene Chloride Dichloromethane This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical Section 3 Physical Chemical Properties Physical Chemical Properties CAS 75 09 2 CI Hazardous Class GHS Classification Acute toxicity oral and dermal Skin and eye irritation Carcinogenicity Specific target
222. ng cryogenic liquids Proper PPE for handling cryogenic liquids includes chemical splash goggles a face shield cryogenic safe gloves long sleeves long pants and closed toe shoes e Cryogenic liquids will vent boil off from their storage containers as part of normal operation Containers are typically of a vacuum jacketed design to minimize heat loss Excessive venting and or an isolated ice build up on the vessel walls may indicate a fault in the vessel s integrity or a problem in the process line A leaky container should be removed from service and taken to a safe well ventilated area immediately e All systems components piping valves etc must be designed to withstand extreme temperatures e Pressure relief valves must be in place in systems and piping to prevent pressure build up Any system section that could be valved off while containing cryogenic liquid must 43 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan have a pressure relief valve The pressure relief valve relief ports must be positioned to face toward a safe location e Transfer operations involving open cryogenic containers such as Dewars must be done slowly while wearing all required PPE Care must be taken not to contact non insulated pipes and system components e Open transfers will be allowed only in well ventilated areas e Donot use a funnel while transferring cryogenic liquids e Use tongs or
223. nical functionality of each station Any deficiencies are repaired either by REM staff or by Purdue Physical 24 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls Facilities maintenance staff If the safety shower or eye wash unit becomes inoperable notify your building deputy immediately 4 2 6 Fire Extinguishers All fire extinguishers should be mounted on a wall in an area free of clutter Each fire extinguisher on campus is inspected on an annual basis by the Purdue Fire Department All laboratory personnel should be familiar with the location use and classification of the extinguishers in their laboratory Ensure that the fire extinguisher being used is appropriate for the type of material on fire before attempting to extinguish any fire Table 4 1 illustrates the fire classification system which should be used to determine the most suitable fire extinguisher for a particular area Laboratory personnel are not required to extinguish fires that occur in their work areas and should not attempt to do so unless e It is asmall contained fire that can be quickly and safely extinguished e g small trash can sized fire e Appropriate training has been received and the individual feels the fire can be safely extinguished and e It is necessary to extinguish a fire in order to exit an area e g fire is blocking an exit If a fire occurs in the laboratory and is extinguished by
224. nown Chemicals ueessssssnsnenensnnsnnsnnnsnnnennnnnnnnnnnnnnennnnnnnnnensene nenn 76 LA Sink and Trash Dispos l 2ue0R Bananen 77 7 8 Sharps Waste eessssssnenensssssnnnnnnnunnnnnnnnnnnnnnnunnnnnnnnnsnnnnnnnunnnnnnsennnnsnnnnnnnnnsnsnnssnnnennnsnsnsnnnnnn 77 7 9 Liquid Chromatography Waste ccccccssssscececececsesssseaeeeseeseseseeeaeseeeeseessessaeeeeeesesseeees 77 Chapter 8 ChemicalSpills siiseicccccsenwscaisnscctieoseussssceuneshevensssdatsadenuesdecssdiseiuaswesdessenasedsscusscesta 79 8 1 Non Emergency Chemical Spill Proc Cures ccccessssececeeecessessaeceeeeeeessessseseeeesesseesees 79 8 2 Emergency Chemical Spill ProceGures ccccccccccccssssssssececeescessessaeeeeeesesssessaeaeeeeeesseseees 79 8 3 SChemicali Spill K Sera aster eadteees era RE ne a Naess 80 Chapter 9 Tr ining auu eier 81 94 CUP Training Rn ie al Denen Sean ease eee akin 81 9 1 1 Annual CHP Refresher Requirements c cccccccecsssesssssececeeecessessaseeeeeeessesseeteaeens 81 9 2 PPE Training ee ei ei an Tara ehe Tee egal ent 82 9 3 SOP Traininge rennen ana mn ernennen a aaa 82 9 4 Laboratory Chemical Safety Course CHM 605 cccccssccccessssececssseceesesseeeeeesssseeeensaaes 83 9 5 REM Researcher S Gulde nass Ringes 83 Appendix A CHP Awareness Certification FOrmM sssssssssssossssoossssccsssecessseseccssssoosssseesesee 84 Appendix B OSHA Hazard Class Definitions c
225. ns 1 Prior to pick up all waste must be placed in a designated area within the room where the waste was generated All waste must be placed in an appropriate container s 3 All containers must be capped and labeled Complete and submit a Hazardous Materials Pickup Request Form Figure 7 6 Visit the REM webpage to find the online Hazardous Material Pickup Request submission form For further information regarding hazardous waste disposal call REM at 765 49 40121 or visit the REM webpage http www purdue edu rem hmm hmm htm HMM 001 Hazardous Material Pickup Request i atmixuremateriais must be submitedina z 5 percentage format Each Container s percentage must Send to REM LMSB via campus mail or Fax 49 61106 add up to 100 See Example Below 2 Chemical descriptions on this form must match the container label PI PI Telephone Always include as much information as possible Only submit known mixtures or chemicals Absolutely no chemical formulae abbreviations Req uested By Date PeT er structures When submitting trade products Telephone E Mail It is your responsibility to provide the product information Mi r x An accurate chemical description of the product Building Room Shop must accompany the form i e an MSDS VANTAO nn Make sure all writing is legible Department Dept Code Forms completed improperly can cause severe delays SI in pickup times Example Pickup Line 4
226. ns require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended Wearing two pairs of nitrile gloves is recommended NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific pyrophoric chemical being used Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx Pyrophoric Materials Date 9 18 2013 http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Face shield is also recommended Skin and Body Protection Flame resistant lab coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equiv
227. ntainer s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Submit Piranha solution for waste pick up as soon as possible The Piranha solution must be cooled and kept in a waste container with a vented cap and in a designated area Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the Piranha solution components SDS must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text Piranha Solution Date 3 14 2014 PURDUE UNIVERSITY NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with Piranha solution designated personnel must provide training to his her laboratory personnel specific to the hazards involv
228. ntaminated clothing and skin with plenty of water before removing clothes Section 6 Engineering Controls Preparation and use of base baths should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician If skin irritation or dermatitis develops seek immediate medical attention In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention Base Baths Date 1 17 2014 PURDUE UNIVERSITY If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person If victim is conscious and alert rinse mouth out with water Seek immediate medical attention Section 8 Special Handling and Storage Requirements e Do not make excessive amounts of base bath solution only make wh
229. ntrinsically safe refrigerators or freezers Store diethyl ether away from light Limit exposure to air and other contaminants specifically heavy metals e f possible only use diethyl ether with inhibitors Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate al
230. o obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific Formaldehyde solution and concentration must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with Formaldehyde designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must
231. ocedure Additional lab protocol may be added here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with Sodium azide designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Sodium Azide Date 3 27 2014 PURDUE UNIVERSITY Standard Operating Procedure Sulfuric acid concentrated This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a
232. oethylene Divinylacetylene Vinylidine chloride Group B chemicals form peroxides that are hazardous only on concentration by distillation or evaporation Test these before distillation and discard after 12 months Table C 2 Group B Chemicals Acetal Dicyclopentadiene Methyl isobutyl ketone Acetaldehyde Diethyl ether 4 Methyl 2 pentanol Benzyl alcohol Diethylene glycol dimethyl ether 2 Pentanol 2 Butanol Dioxane 4 Penten 1 ol Cumene Ethylene glycol dimethyl ether 1 Phenylethanol Cyclohexanol 4 Heptanol 2 Phenylethanol 2 cyclohexen 1 ol 2 Hexanol 2 Propanol Cyclohexene Methylacetylene Tetrahydrofuran Decahydronaphthalene 3 Methyl 1 butanol Tetrahydronaphthalene Diacetylene Methylcyclopentane Vinyl ether 95 The official version of this document will only be maintained online Appendix C Peroxide Forming Chemicals Group C chemicals consist of monomers which form peroxides that can initiate explosive polymerization Inhibited monomers should be tested before use and discarded after 12 months Uninhibited monomers should be discarded 24 hours after opening Table C 3 Group C Chemicals Acrylic acid Styrene Acrylonitrile Tetrafluoroethylene Butadiene Vinyl acetate Chloroprene Vinyl acetylene Chlorotrifluoroethylene Vinyl chloride Methyl methacrylate Vinyl pyridine General Guidelines e Solvents containing inhibitors should be used whenever possibl
233. of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Bromine Date 2 17 2014 PURDUE UNIVERSITY Section 11 Waste Disposal Procedures Label Waste Bromine waste cannot be disposed of down the drain Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area Dispose of Waste
234. of vapor or mist Keep away from incompatible materials such as acids and oxidizing materials Keep away from sources of ignition Avoid heat and shock or friction when handling Containers should remain closed when not in use Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Base Baths Date 1 17 2014 PURDUE UNIVERSITY Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Wast
235. official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy 6 4 3 Respiratory Protection The use of respirators in the laboratory setting is not typically necessary since all work involving hazardous materials must be conducted in a chemical fume hood whenever possible When ventilation is not adequate to provide protection against an inhalation hazard respiratory protective equipment may be necessary There is a variety of respiratory protective equipment available for use but no one device will provide protection against all possible hazards Respirator selection is based on the chemical and process hazard and the protection factors required Respirators are not to be used except in conjunction REM s Respiratory Protection Program This program includes a review of the process to ensure that proper equipment is selected for the job training of all respiratory protective equipment users concerning the methods for proper use and care of such equipment fitting of respirator users when required and medical surveillance of respirator users when required Contact REM at 765 49 46371 with questions about the Respiratory Protection Program or visit the REM webpage http www purdue edu rem home booklets RPP98 pdf 6 4 4 Eye and Face Protection Each affected employee must use appropriate eye and face protection equipment when exposed to hazards from chemical splash flying debris or other exposur
236. often used as a bleach cleaning agent and disinfectant It is an effective cleaning agent of wastewater It can also be used as a rocket propellant Section 4 Potential Hazards Hydrogen peroxide is a strong oxidizer that has a potential to cause a fire or explosion in contact with incompatible materials such as combustibles e g wood paper organic solvents It is corrosive and light sensitive It is toxic if swallowed and corrosive to the eyes and skin Prolonged exposure may cause dermatitis Potential Health Effects Inhalation May be harmful if inhaled Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract Skin May be harmful if absorbed through skin Causes skin burns Eyes Causes eye burns Ingestion Toxic if swallowed CE Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a re
237. onal lab protocol may be added here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with flammable combustible liquids designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Flammable Combustible Liquids Date 7 1 2013 PURDUE UNIVERSITY Stacey Opperwall 10 15 2014 Flammable Combustible Liquids Date 7 1 2013 PURDUE UNIVERSITY Standard Operating Procedure Flammable Solids This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure se
238. oning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention Oxidizers Date 9 5 2013 PURDUE UNIVERSITY In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Section 8 Special Handling and Storage Requirements e Do not over purchase only purchase what can be safely stored in the laboratory Avoid contact with skin eyes and inhalation Avoid inhalation of vapor or mist Avoid formation of dust Keep away from combustible materials Keep away from sources of ignition No smoking Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such a
239. or Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 Tong Ren 765 494 5466 Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Building Room Number Section 2 Type of SOP CI Process XX Hazardous Chemical Section 3 Physical Chemical Properties Physical Chemical Properties CAS 60 29 7 CI Hazardous Class GHS Classification Flammable Liquid Acute Toxicity Skin Irritation Eye Irritation Specific Target Organ Toxicity Molecular Formula C4H100 Form physical state Liquid Color Colorless Diethyl Ether Date 8 19 2013 PURDUE UNIVERSITY Boiling Point 34 6 C Flash Point 40 C Auto Ignition Point 160 C Lower Explosive Limit 1 8 V Upper Explosive Limit 48 V Section 4 Potential Hazards Diethyl ether is an extremely flammable liquid and vapor forming explosive mixtures with air The auto ignition temperature of diethyl ether is 160 C therefore it can be ignited by a hot surface without flame or spark If not stored and handled properly diethyl ether can pose a serious threat to the health and safety of personnel Diethyl ether is peroxide forming and has the potential to form explosive peroxides therefore it is a time sensitive chemical Refer to REM s Time Sensitive Chemicals webpage for more specific informat
240. or questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific lithium alkyl i e butyllithium must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Lithium alkyls Date 2 1 2013 PURDUE UNIVERSITY Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with Lithium alkyls designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date To
241. or to conducting any work with corrosives designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Corrosives Date 10 1 2013 PURDUE UNIVERSITY Standard Operating Procedure Diethyl Ether This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinat
242. ordance with the requirements of the OSHA PPE regulations 29 CFR 1910 132 29 CFR 1910 140 29 CFR 1910 95 As briefly discussed in Chapter 4 of the CHP PPE should never be used in place of engineering and administrative controls 6 2 Scope This Laboratory PPE Policy applies to all personnel that work with or around hazardous chemicals or other safety and health hazards This policy is a part of the larger all encompassing Purdue PPE Policy that applies to all areas not just laboratories of the West Lafayette Campus regional campuses research farms and agricultural centers and related facilities and operations This Laboratory PPE Policy does not cover all potential hazards e g confined space entry welding operations and high voltage in all operations or settings If a laboratory encounters hazards not covered in this Laboratory PPE Policy then refer to the Purdue University personal Protective Equipment PPE Policy for more information or contact REM at 765 49 46731 for assistance http www purdue edu rem home booklets PPEPolicy pdf 6 3 Hazard Assessment The hazard assessment is a process of identifying the hazards associated with a defined task and prescribing PPE along with other relevant protection measures that must be employed to minimize the risk from the hazards Hazard assessments are performed by completing a certification of hazard assessment which is a written document detailing the hazard assessment process for
243. orm which can be used to document reading the CHP Failure to follow the requirements of the CHP could possibly result in injuries fines from regulatory agencies such as OSHA and or disciplinary action 1 4 2 Laboratory Employee Responsibilities All employees e g lab technicians graduate students undergraduate students post doctoral researchers and visiting scientists in laboratories that use handle or store hazardous chemicals must 1 Review and follow the requirements of the CHP Follow all verbal and written laboratory safety rules regulations and SOPs required for the tasks assigned 3 Develop and practice good personal chemical hygiene habits such keeping work areas clean and uncluttered 4 Plan review and understand the hazards of materials and processes in the laboratory prior to conducting work 5 Utilize appropriate measures to control hazards including consistent and proper use of engineering controls administrative controls and PPE 6 Understand the capabilities and limitations of PPE 7 Immediately report all accidents near misses and unsafe conditions to the laboratory supervisor 8 Complete all required REM and or other mandatory safety training and provide written documentation to the laboratory supervisor 9 Participate in the REM managed medical surveillance program when required and 10 Inform the Laboratory Supervisor of any work modifications ordered by a physician as a result of medical
244. orm is required for all laboratories Detailed information regarding the hazard assessment process is presented in Section 6 3 of the CHP e The Carcinogens Reproductive Toxins or Extremely Toxic Chemicals label Toxic Chemicals Label which is illustrated in Figure 4 6 is required if a lab uses or stores any chemicals on the list linked below Contact REM 765 49 46371 to request Toxic Chemicals Labels http www purdue edu rem home booklets crdalist pdf Figure 4 6 Toxic Chemicals Label 27 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls e The Abbreviations Acronyms and Chemical Formulas list is required for all labs that use abbreviations acronyms and or chemical formulas as a means to label chemical containers including secondary containers such as beakers flasks and vials This list which can be found on the REM webpage is not all inclusive and any abbreviations not listed must be added by laboratory personnel http www purdue edu rem home files guide htm There are several other lab postings that may also be required that are not discussed in the CHP particularly if radioisotopes and or biological agents are used in the lab This information should be obtained by reviewing the Radiation Safety Manual and or Biological Safety Manual Additional information regarding lab postings and labels can be found on the REM webpage http www purdue edu rem hom
245. oroughly with plenty of water for at least 15 minutes and consult a physician and dial 911 If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Dial 911 Section 8 Special Handling and Storage Requirements Avoid contact with skin and eyes Avoid inhalation of vapor or mist Use extreme care when handling methyltrichlorosilane which is flammable toxic corrosive and water reactive W liquid A designated storage area must be established for methyltrichlorosilane such as a flammable storage cabinet that does not contain aqueous solutions Methyltrichlorosilane can also be stored with other organic acids Secondary containment should be used as a precautionary measure Methyltrichlorosilane must be segregated from the following chemicals strong acids bases oxidizing agents e g hydrogen peroxide aqueous solutions reacts violently with water to form HCI gas Do not over purchase only purchase what can be safely stored in the laboratory Avoid contact with skin eyes and inhalation Keep away from sources of ignition Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such as oxidizers Use in the smallest practical quantities for the experiment being performed If flammable liquids such as methyltrichlorosilane are stored in refrigerators or freezers these must be specially modified o
246. ounds including organic solvents e Avoid contact with powdered metals zinc iron copper nickel and brass Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and begin first aid procedures Section 7 immediately Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and begin first aid procedures Section 7 immediately Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Hydrogen Peroxide Date 7 12 2013 PURDUE UNIVE
247. ours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Flammable Combustible Liquids Date 7 1 2013 PURDUE UNIVERSITY Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm No flammable or combustible liquids including alcohols are permitted to be poured down the drain Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific flammable combustible liquids being used must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additi
248. outinely e g daily weekly e For operations where spills and contamination are likely e g agarose gel electrophoresis ethidium bromide applications cover work spaces with a bench paper or liner The soiled bench paper should be changed on a routine basis or as needed e All chemical spills must be cleaned up immediately Refer to Chapter 8 of the CHP for detailed chemical spill cleanup procedures e Donot allow materials to accumulate in laboratory hoods and remove used tissues foil gloves or other unnecessary objects immediately after use The safety of the workspace and the hood ventilation may be compromised when excessive chemicals and equipment are kept in hoods e Ensure that all waste e g trash chemically contaminated waste etc is placed in the appropriate containers Do not overfill waste containers e All equipment should be cleaned and returned to storage after each use e Equipment should be stored in a safe and orderly manner that prevents it from falling e Chemical containers must be clean properly labeled and returned to storage upon completion or usage Avoid storing liquids above eye level e Donot store heavy or frequently used items on top shelves Locate items used daily close to the work area 31 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 3 Chemical Inventories It is a prudent practice to develop and maintain a chemical inventory
249. overed by this SOP Building Room Number Section 2 Type of SOP CI Process Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A GHS Classification Oxidizing Liquid or Oxidizing Solid Molecular Formula N A Form physical state Liquid or Solid Color N A Boiling Point N A Oxidizers Date 9 5 2013 PURDUE UNIVERSITY Flash Point N A Lower Explosive Limit N A Upper Explosive Limit N A Relative Vapor Density N A Uses Common oxidizers include Hydrogen peroxide Nitric acid Nitrate and Nitrite compounds Perchloric acid and Perchlorate compounds and Hypochlorite compounds such as household bleach Oxidizers have a wide variety of applications including cleaners and disinfectants agricultural fertilizers rocket propellant and fuel and explosives Important Definitions e Oxidizing liquid a liquid which while in itself is not necessarily combustible may generally by yielding oxygen cause or contribute to the combustion of other material Hydrogen peroxide nitric acid and nitrate solutions are examples of oxidizing liquids commonly found in a laboratory Oxidizing solid a solid which while in itself not necessarily combustible may generally by yielding oxygen cause or contribute to the combustion of other material Section 4 Potential Hazards Oxidizer Keep away from heat Keep away from clothing and other
250. owed GIN ZED Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances Hydrofluoric Acid HF Date 2 1 2013 PURDUE UNIVERSITY e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Natural Rubber arm length or Heavy duty Nitrile arm length gloves is highly recommended Note This type of glove must be put on over the inner Butyl Viton gloves disposable nitrile gloves Gloves must be inspected prior to each use Use proper glove removal technique without touching outer surface of the gloves to avoid skin contact with HF on the contaminated gloves Dispose of inner nitrile gloves after use as hazardous waste Inner Butyl Viton gloves can be reused carefully i e without touching the outer surface of the gloves Wash hands thoroughly with
251. owever laboratory personnel prepare the inner container e g vial jar and provide it to REM for shipment When selecting an inner container to be given to REM for an off campus hazardous materials shipment the following guidelines must be followed e The chemical must be compatible with the container For example corrosive chemicals must not be placed in metal containers hydrofluoric acid in any concentration must not be placed in glass containers e Chemical permeability should be considered when selecting a plastic container especially for organic solvents The container must be able to effectively contain the chemical during transportation under normal conditions e The container must have an appropriate lid that is able to close and seal meaning the container will not leak during transportation under normal conditions Any containers that do not properly seal e g beaker flask test tube will not be shipped off campus by REM 5 14 2 Transporting Chemicals on Campus via Purdue Vehicle Purdue University is a state agency and therefore is exempt from Department of Transportation DOT hazardous materials regulations However the intent of the DOT regulations is still required when transporting chemicals on campus using a motor vehicle This essentially means that all chemical containers must be properly packaged labeled and segregated according to hazard class Do not attempt to move large volumes e g greater than 5 gallon
252. p process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are acceptable for minimal or splash contact only Viton gloves are recommended for full contact exposure Check the resources below for the most suitable glove Sulfuric acid Date 3 19 2014 PURDUE UNIVERSITY NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific chemical being used Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required A face shield may also be appropriate depending on the specific application Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be wor
253. peated exposure to the substance Examples of sensitizers include diazomethane chromium nickel formaldehyde isocyanates arylhydrazines benzylic and allylic halides and many phenol derivatives Sensitizer exposure can lead to all of the symptoms associated with allergic reactions or can increase an individual s existing allergies 90 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions B 2 3 Corrosives Corrosive substances cause destruction of living tissue by chemical corrosion at the site of contact and can be either acidic or caustic basic Major classes of corrosive substances include e Strong acids such as sulfuric nitric hydrochloric and hydrofluoric acids e Strong bases such as sodium hydroxide potassium hydroxide and T ammonium hydroxide e Dehydrating agents such sulfuric acid sodium hydroxide phosphorus pentoxide and calcium oxide e Oxidizing agents such as hydrogen peroxide chlorine and bromine Chapter 5 7 6 of the CHP details corrosives storage requirements B 2 4 Hazardous Substances with Toxic Effects on Specific Organs Substances with toxic effects on specific organs include e Hepatotoxins which are substances that produce liver damage such as nitrosamines and carbon tetrachloride e Nephrotoxins which are substances that cause damage to the kidneys such as certain halogenated hydrocarbons e Neurotoxins which are substances that produc
254. piration date For example hydrogen fluoride HF and hydrogen bromide HBr cylinders have a shelf life of one to two years depending on the vendor Over time moisture can slowly enter the cylinder which initiates corrosion As the corrosion continues HF and or HBr slowly react with the internal metal walls of the cylinder to produce hydrogen The walls of the cylinder weaken due to the corrosion while at the same time the internal pressure increases due to the hydrogen generation Ultimately these cylinders fail and create extremely dangerous projectiles and a toxic gas release Figure 5 8 shows a 30 year old HF lecture bottle cylinder that exploded in a Purdue University laboratory in 2011 42 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Figure 5 8 HF Cylinder Incident at Purdue University in 2011 5 9 Cryogenic Liquids Safety A cryogenic liquid is defined as a liquid with a normal boiling point below 150 C 240 F The most common cryogenic liquid used in a laboratory setting is liquid nitrogen By definition all cryogenic liquids are extremely cold Cryogenic liquids and their vapors can rapidly freeze human tissue and can also pose an asphyxiation hazard if handled in confined spaces The following precautions should be taken when handling cryogenic liquids e Use and store cryogenic liquids in well ventilated areas only e Wear appropriate PPE while handli
255. pirator Methyltrichlorosilane Date 12 13 2013 PURDUE UNIVERSITY e There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile or fluorinated rubber gloves are recommended for low volume applications Wearing two pairs of gloves is recommended Carefully inspect gloves prior to use Check the resources below for the most suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific flammable or combustible liquids being used Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required Only work with this material in a chemical fume hood equipped with a sash that protects the worker s face and eyes from splash Skin and Body Protection Laboratory coats must be worn and be appropriately si
256. plash goggles Face shield is also recommended if there is a high probability of a splash hazard Skin and Body Protection Lab coats 100 cotton must be worn and be appropriately sized for the individual and buttoned to their full length Flame resistant lab coats should be worn if handling more than 1 liter of benzene Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel must also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle must not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of Benzene must be conducted in a properly functioning chemical fume hood The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Consult a physician In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Consult a physician In case of eye contact Check
257. plete and return a copy of this form to your principal investigator supervisor By signing below you acknowledge that you are aware of the Chemical Hygiene Plan and the policies and procedures applicable to the OSHA Occupational Exposure to Hazardous Chemicals in Laboratories Standard 29 CFR 1910 1450 Your supervisor will provide additional information and training as appropriate Name Work Telephone Email Address Department Job Title Employee Signature Date Filing Completed Chemical Hygiene Plan Awareness Certifications are to be filed in a central administrative location within each staff member s department These and all other safety training records should be organized in a way that allows original records to be retrieved quickly and efficiently on request by an OSHA inspector or a REM staff member and to be retrieved for a single staff member or for an entire work group identified by PI supervisor 85 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions Appendix B OSHA Hazard Class Definitions 86 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions B 1 Physical Hazards B 1 1 Flammable Liquids Flammable hazards are materials which under standard conditions can generate sufficient vapor to cause a fire in the presence of an ignition source Flammable liquids
258. quantity PPE Figure 2 Intermediate Figure 3 Large quantity PPE requirements quantity PPE requirements requirements Section 6 Engineering Controls Liquid nitrogen must only be used in a well ventilated area or in a properly functioning chemical fume hood whenever possible Liquid nitrogen should never be used in a poorly ventilated enclosed area where oxygen displacement is a possibility Liquid Nitrogen Date 5 9 2014 PURDUE UNIVERSITY Section 7 Liquid Nitrogen Containers Vacuum Insulated Containers Vacuum insulated containers are used for storing and dispensing liquid nitrogen They are either sealed capable of holding 20 psig 240 psig with pressure relief valve or ambient pressure covered loosely with a cap cork or stopper and are referred to as Dewars due werz There are two primary types of Dewars benchtop and large Dewars Benchtop Dewars Figure 4 are typically for small scale laboratory use and the lid is the only pressure relief device Large Dewars Figure 5 are typically used for storage of lab samples movement of samples between campus locations or to fill other secondary containers Large Dewars may have a secure seal and pressure relief vent Liquid nitrogen exposure is a risk when handling Dewars Therefore the PPE illustrated in Figure 2 should be worn when working with a Dewar Figure 4 Benchtop Dewar Figure 5 Large Dewar Cryogenic Tubes Cryogenic tubes are typically us
259. r purpose made flammable safe refrigerators and freezers which have no internal sources of ignition posed by an internal light or thermostat circuit Any expired or unnecessary reactive materials should be properly disposed of as hazardous waste Note In case you need to dilute the concentration of acids always add acid to water Conduct the procedure detailed in Section 13 of this SOP only after a supervisor has observed the user performing the proper technique unassisted Do not work with dangerous when wet materials alone Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Methyltrichlorosilane Date 12 13 2013 PURDUE UNIVERSITY Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10
260. ration LC50 in air of 200 parts per million ppm by volume or less of gas or vapor or 2 mg per liter or less of mist fume or dust when administered to certain test populations by continuous inhalation for one hour provided such concentration and or condition are likely to be encountered by humans when the chemical is used in any reasonably foreseeable manner Chapter 5 7 5 of the CHP details acutely toxic compounds storage requirements 93 The official version of this document will only be maintained online Appendix C Peroxide Forming Chemicals Appendix C Peroxide Forming Chemicals 94 The official version of this document will only be maintained online Appendix C Peroxide Forming Chemicals Autoxidation in common laboratory solvents can lead to unstable and potentially explosive peroxide formation The reaction can be initiated by exposure to air heat light or contaminants Most of these solvents are available with inhibitors to slow the peroxide formation Examples of inhibitors include BHT 2 6 di tert butyl 4 methyl phenol and Hydroquinone There are three categories of peroxide formers Group A chemicals are those which form explosive levels of peroxides after prolonged storage especially after exposure to air without concentration Test these for peroxide formation before using and discard 3 months after opening Table C 1 Group A Chemicals Butadiene Isopropyl ether Chloroprene Tetrafluor
261. rators should be used under any of the following circumstances Flammable Combustible Liquids Date 7 1 2013 PURDUE UNIVERSITY e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended for low volume applications Wearing two pairs of nitrile gloves is recommended If handling a high volume gt 4 liters of flammable or combustible liquid then disposable gloves are likely not suitable a more heavy duty glove such as a butyl rubber is required Check the resources below for the most suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific flammable or combustible liquids being used Refer to glove selection chart from the links below ht
262. ratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Add lab specific Protocol Procedure here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with pyrophoric materials designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year Pyrophoric Materials Date 9 18 2013 PURDUE UNIVERSITY have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10
263. refrigerators or freezers should all have suitable plastic trays for secondary containment in the refrigerator and freezer compartments If plastic trays are not available liquid chemicals should be placed in secondary containers to contain spills e Remember that power outages and technology failures can cause internal temperatures to rise which can impact chemical contents Be aware of unusual odors vapors etc when opening the refrigerator or freezer e Aninventory should be posted on the refrigerator door e Chemical refrigerator or freezers should be located away from laboratory exits e Refrigerators and freezers should be cleaned out and manually defrosted as necessary e When defrosting a freezer consideration should be taken regarding potential chemical contamination of the water If the water draining from a defrosted refrigerator may be chemically contaminated contact REM at 765 49 40121 for further assistance Compressed Gas Cylinder Safety Compressed gas storage requirements are discussed above in Section 5 7 3 However there are additional important safety requirements for use of compressed gases in laboratories detailed below Gas cylinder connections and fittings must be inspected frequently for deterioration Never use a leaking corroded or damaged cylinder and never refill compressed gas cylinders When stopping a leak between cylinder and regulator always close the valve before tightening the union nut
264. rgency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Flammable Solids Date 11 13 2013 PURDUE UNIVERSITY Standard Operating Procedure Formaldehyde 37 Solution This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Principal Investigator Internal Lab Safety Coordinator Lab Manager Lab Phone Office Phone Emergency Contact Location s covered by this SOP Chemistry 9 26 2014 9 26 2014 Tong Ren Sean Natoli 765 494 4573 765 494 5466 To
265. rincipal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Hydrogen Peroxide Date 7 12 2013 PURDUE UNIVERSITY Name Signature Date Stacey Opperwall 10 15 2014 Hydrogen Peroxide Date 7 12 2013 PURDUE UNIVERSITY Standard Operating Procedure Liquid Nitrogen This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone 765 494 4573 Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s covered by this
266. rly Do not attempt to move large volumes e g greater than 5 gallons in total volume of chemicals across campus via foot If a large volume of chemicals needs to be moved such as an entire lab move contact REM 765 49 40121 for further assistance The following procedures must be followed when moving chemicals on campus by way of foot PPE must be worn when handling potentially contaminated surfaces During the time which the chemicals are moved on campus via foot PPE may not be necessary or even appropriate e g employees should not wear chemical resistant gloves in public areas However appropriate PPE and spill containment equipment should be brought along in the event of a spill or incident Purdue Stores stock room personnel shall not dispense or sell chemicals in breakable containers of any size unless the customer has an approved transport container in which to place the chemical for transporting before leaving the Stock Room Chemical requisitioners may purchase a transport container from Purdue Stores Approved transport container means a commercially available bottle carrier made of rubber metal or plastic with carrying handle s which is large enough to hold the contents of the container if broken in transit Carrier lids or Figure 5 10 Chemical Bottle Carrier covers are recommended but not required Rubber or plastic should be used for acids alkalis and metal rubber or plastic for organic solvents An
267. rmal tissue after repeated exposure to the substance 17 The official version of this document will only be maintained online Chapter 2 Classes of Hazardous Chemicals 3 3 Biological Hazards The Purdue University Institutional Biosafety Committee IBC is the campus based committee that has the responsibility for reviewing and approving all proposals activities and experiments involving an organism or product of an organism that presents a risk to humans plants animals or the environment The PI must submit to the IBC an application to use rDNA synthetic nucleic acids potential pathogens human tissue fluids and or cell lines in their research The IBC review is conducted in accordance with the guidance and requirements of National Institutes of Health the Centers for Disease Control and Purdue University policies and the Biosafety Manual All Pls have an obligation to be closely familiar with EHS guidelines applicable to their work and to adhere to them More detail regarding the IBC process can be found on the Purdue Office of the Vice President for Research webpage http www purdue edu research vpr rschadmin rschoversight rdna forms php 3 4 Radioactive Material Hazards The Purdue University Radiation Safety Committee RSC is the campus based committee that has the responsibility for reviewing and approving all proposals activities and experiments involving radioactive material and radiation producing devices The Pl must
268. rovided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Sulfuric acid Date 3 19 2014 PURDUE UNIVERSITY Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Sulfuric acid Date 3 19 2014 Tab 2 Lab Specific Protocols Requirements Rules Tab 2 Lab Specific Protocols Requirements Rules Tab 3 Lab Specific Hazard Assessments
269. rovided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Nitric Acid Date 7 2 2013 PURDUE UNIVERSITY Signature Date Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Nitric Acid Date 7 2 2013 PURDUE UNIVERSITY Standard Operating Procedure Oxidizers This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Chemistry Date SOP was written 9 26 2014 Date SOP was approved by PI lab supervisor 9 26 2014 Principal Investigator Tong Ren Internal Lab Safety Coordinator Lab Manager Sean Natoli Lab Phone 765 494 4573 Office Phone 765 494 5466 Tong Ren 765 494 5466 Emergency Contact Name and Phone Number BRWN 5134 5134A 5150D 5130A 5132 5134B 5144 5148 Location s c
270. s e Are my storage facilities appropriate for the type of materials will be using e Can properly segregate incompatible chemicals e What possible accidents can occur and what steps can take to minimize the likelihood and impact of an accident What is the worst incident that could result from my work 29 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 1 2 General Laboratory Safety Rules It is extremely important that all laboratory safety rules are known and followed by lab personnel Not only is it important that the rules are understood and followed it is also important that the Laboratory Supervisor enforce all lab safety rules A culture of safety must be adopted by all employees before a lab safety program can be successful The following general laboratory safety rules should be followed at all times e Prior to beginning work in the lab be prepared for hazardous materials emergencies and know what actions to take in the event of an emergency Plan for the worst case scenario Be sure that necessary supplies and equipment are available for handling small spills of hazardous chemicals Know the location of safety equipment such as the nearest safety shower and eyewash station fire extinguisher spill kit and fire alarm pull station e Do not work alone in the laboratory if you are working with high hazard materials e g acutely toxics reactives or processes
271. s including beakers flasks vials and test tubes The label should be placed on the container not the cap to avoid accidental mislabeling e Immediately replace labels that have fallen off or that are deteriorated e Label containers using chemical names Do not use abbreviations structure or formulae e Archived research samples are often stored in boxes containing hundreds of small vials Label the outside of the box with the chemical constituents paying special attention to regulated materials such as radioactive material organic solvents heavy metals and other toxics If the samples are nonhazardous label them as such e Submit frequent Hazardous Materials Pickup Request Forms to reduce the amount of chemicals in your laboratory e Employees should dispose of all of their waste before leaving graduating from Purdue The lab and or department should come up with a system to ensure that all faculty staff and students properly dispose of hazardous waste including unwanted research samples before employees leave 76 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 7 Sink and Trash Disposal No chemical waste should be poured down the drain or discarded in the trash unless it is certain that doing so does not violate hazardous waste regulations or the West Lafayette wastewater treatment plant s discharge requirements In order to ensure improper disposal does not occur
272. s document will only be maintained online Chapter 6 Laboratory PPE Policy See REM webpage for the location evaluation hazard assessment template http www purdue edu rem home files forms htm ppe 6 3 3 Job Title Evaluation Hazard Assessment Job title evaluation hazard assessments should be conducted for specific positions These types of hazard assessments should be written in a comprehensive manner that includes the majority of hazards that a specific job position e g Animal Care Technician routinely encounters during the normal course of work This type of hazard assessment is commonly used for positions where the hazards encountered do not frequently change If the employee encounters a hazard that is not covered by the job title evaluation hazard assessment then it will be necessary to perform another type of hazard assessment such as the task evaluation hazard assessment that does address the specific hazards of that task The following describes the steps that should be taken to perform a task evaluation hazard assessment e Identify hazards that the position title may encounter while performing normal duties e List each task where hazard is present e Determine PPE requirements for each task e List other control measures required See the REM webpage for the job title evaluation hazard assessment template http www purdue edu rem home files forms htm ppe 6 4 Minimum PPE Requirements for Laboratories This section
273. s flammable and combustible liquids Keep cool and protected from sunlight e Opened containers of oxidizing liquids must be carefully resealed and kept upright to prevent leakage e Carefully follow manufacturer s instructions if oxidizing liquid needs to be vented during storage Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Oxidizers Date 9 5 2013 PURDUE UNIVERSITY Section
274. s in total volume of chemicals across campus If a large volume of chemicals needs to be moved across campus such as an entire lab move contact REM 765 49 40121 52 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan for further assistance The following procedures must be followed in order to properly and legally transport chemicals across campus e Only Purdue owned vehicles are permitted to be used to transport chemicals For liability and insurance purposes no personal vehicles should ever be used to transport hazardous chemicals e Ensure that each container has an appropriate tight fitting lid The lid should have the ability to contain the contents of the container even if it becomes inverted during transport Examples of inappropriate lids include cracked caps loosely fitting rubber stoppers or Parafilm e Chemicals should be segregated according to the primary hazard class For example do not place an oxidizer such as ammonium nitrate in the same container as an organic solvent such as acetone e All containers should be packaged upright e Chemical containers should be placed in some type of outer packing such as a box bin or bucket Containers should remain securely packaged during loading transport and unloading Glass to glass contact should be avoided Bubble wrap newspaper and vermiculite are good examples of packaging material that will prevent glass to gl
275. s is required e Prior to conducting any work with Methylene chloride designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Name Signature Date Tong Ren 10 15 2014 Sean Natoli 10 15 2014 Tim Cook 10 15 2014 Sarah Tyler 10 15 2014 Ashlin Porter 10 15 2014 Eileen Judkins 10 15 2014 Stacey Opperwall 10 15 2014 Methylene Chloride Date 9 26 2013 PURDUE UNIVERSITY Standard Operating Procedure Methyltrichlorosilane This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Section 1 Lab Specific Information Department Date SOP was written Date SOP was approved by PI lab supervisor Pri
276. s required to improve conditions Refer to the REM webpage for detailed information regarding REM s laboratory ergonomics program http www purdue edu rem safety ergo htm 5 18 Laboratory Electrical Safety 5 18 1 Training Laboratory employees shall be trained to understand the specific hazards associated with electrical energy See the written Electrical Safety Program on the REM website for more detailed information http www purdue edu rem home booklets elsp pdf Employees who need access to operate circuit breakers and fused switches in electrical panels may require additional training to be designated by their supervisor as qualified for the task 5 18 2 Portable Electrical Equipment and Extension Cords The following requirements apply to the use of cord and plug connected equipment and flexible cord sets extension cords 56 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Extension cords may only be used to provide temporary power and must be used with Ground Fault Circuit Interrupter GFCI protection during maintenance and construction activities and in damp or wet locations Portable cord and plug connected equipment and extension cords must be visually inspected before use for external defects such as loose parts deformed and missing pins or damage to outer jacket or insulation and for possible internal damage such as pinched or crushed outer jacket Any
277. s should remain closed when not in use Containers should be labeled appropriately Label should indicate the name of the chemical s in the container Avoid using chemical abbreviations acceptable if a legend is present in the lab and formulae Containers should be in good condition and compatible with the material Avoid using ignition sources flame burners or any open flame source hot plates electrical equipment with frayed or cracked wiring etc and or creating static electricity in areas where oxidizing chemicals are being used Transport all oxidizing liquids and solids in secondary containment such as polyethylene or other non reactive acid solvent bottle carrier Oxidizers must be segregated from incompatible materials such as flammable and combustible materials Incompatibilities will be noted in Section 10 of the SDS Stability and Reactivity Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with oxidizers designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures Oxidizers Date 9 5 2013 PURDUE UNIVERSITY e The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manuf
278. scious person Section 8 Special Handling and Storage Requirements Ensure that you have all the PPE required for handling HF HF must always be stored in plastic nalgene polypropylene containers Do not store HF in glass bottles containers Store in corrosive acid lab storage cabinet within a secondary containment nalgene polypropylene tray or tub Hydrofluoric Acid HF Date 2 1 2013 PURDUE UNIVERSITY e Do not store in the top most shelf of the storage cabinet In general do not store chemicals at or above eye level Ensure the container is tightly closed at all times Do not store with oxides organic chemicals bases or metals Carefully carry the stock bottle in a rubber maid bottle carrier nalgene secondary container to the wet bench chemical fume hood and pour out desired amount into a smaller container Place stock bottle back in corrosive chemical storage cabinet with cap tightly closed Lab buddy system is highly recommended when handling HF Lab emergency contact information must be readily available The lab personnel must have easy access to a telephone landline or cell phone Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM
279. sed as a polymerization initiator in the production of elastomers such as polybutadiene or styrene butadiene styrene SBS Also it is broadly employed as a strong base in organic synthesis both industrially and in the laboratory Lithium alkyl compounds are also used for the exchange of lithium with halogens and for the deprotonation of amines and activated C H compounds Section 4 Potential Hazards Lithium alkyls are classified as flammable liquids pyrophorics and water reactives They react violently with water liberating extremely flammable gas Spontaneously flammable in air and causes burns Dangers of serious damage to health by prolonged exposure through inhalation Pose a possible risk of an impaired fertility Extreme caution is advised Keep away from heat and sources of ignition Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract Vapors may cause drowsiness and dizziness While it is possible to work with this compound using cannula transfer traces of these compounds at the tip of the needle or cannula may catch fire and clog the cannula with lithium salts Some workers prefer to enclose the needle tip or cannula in a short glass tube which is flushed with an inert gas and sealed via two septa Examples include tert butyllithium and n butyllithium D Am Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any
280. should be used in power stirring and mixing devices or any other rotating equipment used for laboratory operations Because stirring and mixing devices especially stirring motors and magnetic stirrers are often operated for fairly long periods without constant attention the consequences of stirrer failure electrical overload or blockage of the motion of the stirring impeller should be considered 5 12 3 Heating Devices Laboratories commonly use heating devices such as ovens hot plates heating mantles oil baths salt baths sand baths air baths hot tube furnaces hot air guns and microwave ovens Steam heated devices are generally preferred whenever temperatures of 100 C or less are required because they do not present shock or spark risks and can be left unattended with assurance that their temperature will never exceed 100 C Ensure the supply of water for steam generation is sufficient prior to leaving the reaction for any extended period of time A number of general precautions need to be taken when working with heating devices in the laboratory When working with heating devices consider the following 46 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e The actual heating element in any laboratory heating device should be enclosed in such a fashion as to prevent a laboratory worker or any metallic conductor from accidentally touching the wire carrying the ele
281. skin It is extremely destructive to the tissue of the mucous membranes and upper respiratory tract Causes severe skin and eye burns May cause blindness and permanent eye damage Inhalation may cause spasms inflammation and edema of the bronchi or larynx Other symptoms include burning sensation coughing wheezing shortness of breath headache nausea vomiting and pulmonary edema Effects may be delayed Large doses may conversion of hemoglobin to methemoglobin producing cyanosis or a drastic fall in blood pressure leading to collapse coma and possibly death Chronic exposure may cause erosion of the teeth jaw necrosis and kidney damage Potential Health Effects Inhalation May be harmful if inhaled Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract Skin May be harmful if absorbed through skin Causes skin burns Eyes Causes eye burns Causes severe eye burns Ingestion May be harmful if swallowed A LET Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harm
282. skin exposure while wearing gloves Personnel must also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle must not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of potassium cyanide must be conducted in a properly functioning chemical fume hood The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Dial 911 Potassium Cyanide Date 11 26 2013 PURDUE UNIVERSITY In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Dial 911 In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes Dial 911 If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Dial 911 Section 8 Special Handling and Storage Requirements e A designated s
283. sorbed through skin May cause skin irritation May cause eye irritation May be fatal if swallowed May react with metal spatulas and metal lab equipment to form shock sensitive salts Sodium azide reacts with Lead Copper Silver Gold and metal halides to form heavy metal azides which are explosive Contact with acids liberates very toxic gas gt Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Chemical resistant gloves must be worn Handle with nitrile or chloroprene gloves Double gloving is recommended when working with pure Sodium azide or Sodium azide solutions greater than 5 Use proper glove removal technique to avoid any skin contact Check the resources below and the SDS for
284. spill response kit available for use Lab spill kits can either be purchased from a vendor or created by lab personnel but each spill kit should be equipped to handle small spills of the most common hazards in the laboratory The kit should be equipped with response and cleanup materials such as e Absorbent materials such as pads booms oil dry or kitty litter booms or pillows e Neutralizing agents e g Neutrasorb for acids and or bases if high volume of acids and or bases are stored in the laboratory e Containers such as drums buckets and or bags to containerize spilled material and contaminate debris generated during the cleanup process e PPE such as gloves safety glasses and or goggles lab coat or apron chemical resistant booties e Caution tape or some other means to warn people of the spill 80 The official version of this document will only be maintained online Chapter 9 Training Chapter 9 Training Effective training is crucial to a successful laboratory safety program Laboratory Supervisors must actively participate in the training process to ensure that all lab employees are effectively trained before any work with hazardous materials occurs This chapter details the minimum training requirements for all Purdue laboratories It should be noted that depending on the type of research being conducted and associated hazards there may be additional training requirements that are not detailed in this chapter For
285. spirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Handle with gloves Nitrile gloves are recommended Two pairs of nitrile gloves are recommended Hydrogen Peroxide Date 7 12 2013 PURDUE UNIVERSITY NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Hydrogen peroxide Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or goggles are required A face shield is also recommended Skin and Body Protection Lab coat full length pants and closed toe shoes are required Hygiene Measures Avoid contact with skin eyes and clothing Wash hands before breaks and immediately after handling the product Section 6 Engineering Controls Use of hydrogen peroxide should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek
286. spital If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek medical attention immediately Section 8 Special Handling and Storage Requirements e Precautions for safe handling water reactive use extreme care when handling Alkali metals should be stored in mineral oil or kerosene Only handle under inert gas use a glove box if possible Do not expose to air Avoid contact with skin and eyes and inhalation A dry run of the experiment should be performed using low hazard materials Never work with alkali metals alone Conduct the procedure only after a supervisor has observed the user performing the proper technique unassisted Alkali Metals Date 9 26 2013 PURDUE UNIVERSITY e All glassware used for alkali metals should be oven dried and free of moisture e Keep away from sources of ignition e Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such as aqueous solutions and halogenated alkanes i e carbon tetrachloride The amount of alkali metals stored should be kept at a minimum Any expired or unnecessary reactive materials should be properly disposed of as hazardous waste All alkali metals should be clearly labeled with the original manufacturer s label which should have the chemical name hazard labels and pictograms The label should not be defaced in any way All
287. ssaa dace 7 1 7 Chemical and Laboratory Safety Committee uueneeennsseesssnnnnensnnnnnnnnnnnnnnnnnnnnnnnnnnnnnsnnnnnnnnnn 8 Chapter 2 Chemical Classification Systems ccsssssccccsssssceccssssssccccssssceccenssceceeceseeceeeessseees 9 2 1 Globally Harmonized System for Classifying Chemicals ccccssscceceessseeeesssteeeesssteeeens 9 231 1 Safety Data SECS sien sie nenn ai Seacees fi poo taxa tea eee is tog ES 9 2142 Chemical Labeling antenne vee T TOET TE 10 2 2 National Fire Protection Association Rating System uuesessnneeeessnnnneensnnnnnnnnennnnn essen 13 2 3 Department of Transportation Hazard Classes ccccccccccccssssssssseeeeeeseessessaeseeeesesseeeees 14 Chapter 3 Classes of Hazardous Chemmicalls sssccccssssceccccssssccccssssceccecsscececcssssceceessscees 16 Selby Physical Hazard Seisin sage einen 16 3 2 Health Hazards ao2nn ee ee 17 3 3 1Biol gical Hazards u teas een ea een end 18 3 4 Radioactive Material Hazards cccccssssccccsssscecssseneececseaececsesneeecseseeaececseaeeeceeseeeeeessaaes 18 35 kaser Hazard Seer an Visca Ae aAA AET EE AAEE EAA 18 Chapter 4 Laboratory Safety Controls cccccsssssceccssssceccccssssccccesssceccecssceesccessscsceessseees 19 2 7 Rout S Of EXPOSU En aa a elses A aaa aa a A AA a EA RETEA Ei 19 4 2 Engineering Controls and Safety Equipment neeeeessssnseenennnnennennnnnnnunnnnnnnnnnnnnnnnnnnnnnennn 19 4 2 1 Chemical Fume
288. ssments to effectively control or reduce hazards e Ensure that all laboratory personnel that work with hazardous chemicals receive appropriate training refer to Chapter 9 for detailed training requirements e Maintain written records of laboratory specific training e g PPE training e Ensure that appropriate PPE e g laboratory coats gloves eye protection etc and engineering control equipment e g chemical fume hood are made available in good working order and being used properly e Conduct periodic lab inspections and immediately take steps to abate hazards that may pose a risk to life or safety upon discovery of such hazards and e Actively enforce all applicable safety procedures and ensure that the CHP is followed by lab staff and all visitors including having a progressive disciplinary process for lab staff members that do not comply with safety rules Laboratory Supervisors must ensure that employees receive CHP training and information before any work with hazardous materials occurs Laboratory Supervisors must also ensure 4 The official version of this document will only be maintained online Chapter 1 Introduction that all employees receive annual CHP refresher training The Laboratory Supervisor can provide the training or delegate this task to a qualified individual e g Laboratory Safety Officer senior lab employee The CHP training must be documented See Appendix A for the CHP Awareness Certification F
289. submit to the RSC through REM an application to use radioactive material or radiation producing devices Use of radioactive materials at Purdue University is authorized under a license issued by the US Nuclear Regulatory Commission or a registration with the Indiana State Department of Health and all work must comply with applicable regulations The policies and procedures for handling radioactive materials are contained in the Purdue University Radiation Safety Manual http www purdue edu rem home booklets radman pdf 3 5 Laser Hazards The Purdue University Laser Safety Committee LSC is the campus based committee that has the responsibility for reviewing and approving all proposals activities and experiments involving laser radiation devices Pls must submit to the LSC through REM an application to use Class 3B and Class 4 lasers or laser devices The use of lasers is subject to OSHA regulations and utilizes current ANSI standards to develop guidance The policies and procedures for handling lasers are contained in the Purdue University Laser Safety Guidelines http www purdue edu rem home booklets laserguide pdf 18 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls Chapter 4 Laboratory Safety Controls Laboratory safety controls include engineering controls administrative controls and PPE Elements of these three categories should be used in a layered approach to minimize
290. surveillance occupational injury or chemical exposure 1 4 3 Laboratory Safety Officer Responsibilities Very often it is not practical for the Laboratory Supervisor PI to be present in the lab on daily basis to ensure that safe and compliant practices are being carried out by all lab staff For this reason it is highly recommended that each PI establish a Laboratory Safety Officer to manage the daily operations of the lab s safety program The PI should empower the Laboratory Safety Officer to make decisions on daily operations involving safety and compliance including the authority to instruct other lab personnel to follow all safety 5 The official version of this document will only be maintained online Chapter 1 Introduction procedures e g PPE use hazardous waste procedures etc This person should be familiar with how the lab operates and have demonstrated lab safety experience e g senior graduate student post doc lab manager Having a Laboratory Safety Officer in each lab provides many benefits such as e Other lab personnel know who to contact with questions about daily operations involving safety and compliance e Empowers someone other than the Pl to enforce lab safety rules e Provides consistency within the respective academic department idea is that each Laboratory Safety Officer attends departmental safety committee meetings and reports issues back to the lab and e Provides good marketable experience for
291. t a physician If inhaled move person to fresh air If not breathing give artificial respiration In case of skin contact wash with soap and plenty of water In case of eye contact rinse thoroughly with plenty of water for at least 15 minutes If swallowed do not induce vomiting Never give anything by mouth to an unconscious person Rinse mouth with water consult a physician Highly flammable liquid and vapor Causes mild skin irritation Causes serious eye irritation May cause drowsiness or dizziness Figure 2 3 GHS Label Format As mentioned earlier one of the objectives of GHS was to create a quantitative hazard classification system numerical hazard classification is based on a 1 5 scale 1 being the most hazardous and 5 being the least hazardous based on physical characteristics such as flash point boiling point lethal dose of 50 of a population reactivity etc Table 2 1 illustrates how the numerical hazard classification works for flammable liquids More detailed information on GHS can be found on the OSHA website https www osha gov dsg hazcom ghs html 12 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Table 2 1 GHS Hazard Classification System for Flammable Liquids Flash point lt 23 C Extremely flammable Danger Boiling point lt 35 C liquid and vapor Flash point lt 23 C Highly flammable liqui
292. technique to avoid any skin contact Nitrile gloves layered underneath butyl rubber gauntlet style gloves are recommended Check the resources below for the most suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific base bath solution being used Base Baths Date 1 17 2014 PURDUE UNIVERSITY Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required A face shield may also be necessary when there is a potential for splashes Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Aprons may also be appropriate depending on the application Hygiene Measures Wash thoroughly and immediately after handling Rinse immediately co
293. tes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Pyrophoric Materials Date 9 18 2013 PURDUE UNIVERSITY Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Pyrophoric waste should be segregated from all incompatible chemicals such as aqueous solutions Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for the specific pyrophoric compound i e butyllithium being used must be made available to all personnel working in the labo
294. that involve handling a large volume of flammable materials gt 1 liter e f working with a high hazard chemical ensure that others around you know what you are working with and understand the potential hazards e Limit access to areas where chemicals are used or stored by posting signs and or locking doors when areas are unattended e Purchase the minimum amount of hazardous materials necessary to efficiently operate the laboratory e Ensure that adequate storage facilities e g chemical storage rooms flammable safety cabinets and containers are provided for hazardous materials Ensure that hazardous materials are properly segregated by chemical compatibility e Ensure that ventilation is adequate for the chemicals being used Understand how chemical fume hoods function and be able to determine if the hood is not functioning properly e Use good personal hygiene practices Keep your hands and face clean wash thoroughly with soap and water after handling any chemical e Smoking drinking eating and the application of cosmetics are forbidden in areas where hazardous chemicals are in use Confine long hair and loose clothing e Never smell or taste a hazardous chemical Never use mouth suction to fill a pipette e When using equipment that creates potential hazards e g centrifuge ensure that the equipment is being used following the manufacturer s guidelines and instructions If equipment requires routine maintenance e g HEPA
295. the Laboratory Safety Officer to be involved in a safety leadership role The role of the Laboratory Safety Officer should include 1 Provide training to new lab personnel ensure appropriate training is given and that the training is properly documented Enforce lab safety rules Attend departmental college level safety committee meetings and report significant information back to the lab and 4 Report safety issues back to the PI when necessary 1 4 4 Non Laboratory Personnel Support Staff Responsibilities Custodians and maintenance staff support staff often must enter laboratories to perform routine tasks such as cleaning and equipment maintenance Support staff members are expected to follow the posted safety rules of each laboratory Minimum PPE requirements for support staff working in a laboratory are safety glasses long pants and closed toe shoes If additional PPE is required or if other unique safety requirements must be followed it is the lab personnel s responsibility to notify support staff 1 4 5 Chemical Hygiene Officer Responsibilities The Chemical Hygiene Officer who is the Director of the Purdue Radiological and Environmental Management Department or designated individual s has the primary responsibility for ensuring the implementation of all components of the CHP The Chemical Hygiene Officer must 6 The official version of this document will only be maintained online Chapter 1 Introduction e Infor
296. the most suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Sodium azide Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Sodium Azide Date 3 27 2014 PURDUE UNIVERSITY Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel must also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle must not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of Sodium azide should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures
297. tification Form must be completed for all lab employees This instruction and information box should remain This model Chemical Hygiene Plan is the 2014 version the most current version can be found on the Forms page at www purdue edu rem The official version of this document will only be maintained online Table of Contents Table of Contents CHP D cument Acronyms List enger Se nee 1 Chapter 1 Introduction en een 2 1 1 Purpose 4er onia rk HI Reise E a a a ao aa a 2 A AEs E0 E1 ee T E A os vo A A A E T 2 1 3 CHP USE INSEFUCTIONS anian a a e e a a e a ea t 3 1 4 Employee Rights and Responsibilities ccccccessscccecececsesenseeececeeeeessessaeeeeeeseseessesseaeess 3 1 4 1 Laboratory Supervisor Responsibilities 22 40000022222022000000nnnnnnnennnnsnennnnnnennennnnnn 4 1 4 2 Laboratory Employee Responsibilities cccccssscccccccecessssseceeeceesesssessaeeeeeeseseeesees 5 1 4 3 Laboratory Safety Officer Responsibilities 0u00002222202sssnenenennnnsennnnnsnennnnnnennennnnnn 5 1 4 4 Non Laboratory Personnel Support Staff Responsibilities cccesecssseeesseeeeeees 6 1 4 5 Chemical Hygiene Officer Responsibilities cccccccccccccssssssscececeeecessessaeeeseeeeseeeeees 6 1 5 Radiological amp Environmental Management Department uesssennennessnnnneensnnnnnennnnnnnn 7 1 6 Integrated Safety Plannin arrra nn sneinen a Nenner sveasa led Mv Peaeive
298. tilated and low traffic areas e Caution signs should be posted in the area warning others that liquid nitrogen is being stored and used Liquid Nitrogen Date 5 9 2014 PURDUE UNIVERSITY e Always wear the appropriate PPE e Liquid nitrogen should only be stored in approved containers e All liquid nitrogen containers must be labeled Large containers e g Dewars Self Pressurizing Tanks must be labeled with the REM provided label illustrated in Figure 8 Smaller containers such as cryogenic tubes should be labeled Liquid Nitrogen Cryogenic Hazard or with similar words that convey the hazards Avoid breathing liquid nitrogen vapors Carry containers away from body and face Never drop a liquid nitrogen container Damage to a container may result in over pressurization or container failure Dewars more than 100 pounds require two people to move safely LIQUID NITROGEN Always use a specially designed cylinder cart to transport liquid nitrogen Bldg Room containers that are too heavy to be hand carried Tare Weight Use the freight elevator whenever possible Hinge Warning Contains refrigerated Do not leave open containers unattended Bu a oe en Liquid nitrogen containers should be stored in cool dry and well Handiine Procedures Eek Beoulomants Only use containers approved for LN f Cryogenic gloves venti ated areas Use in well ventilated area Goggles and face shield Transfer liquids slowly to
299. tion of the blood weak and irregular heartbeat collapse unconsciousness convulsions coma and death Inhalation of high concentrations may cause central nervous system effects and can be fatal Prolonged repeated exposure may cause skin necrosis and ulceration of the skin Cyanide acts by inhibiting cytochrome oxidase impairing cellular respiration Chronic exposure to cyanide solutions may lead to cyanide rash with itching and vesicular eruptions with secondary infection Small amounts of cyanide over long periods of time causes loss of appetite headache weakness and respiratory irritation Exposure Limits e Acute toxicity Oral LDLO human 2 857 mg kg LDLO is the lowest dosage per unit of bodyweight of a substance known to have resulted in fatality LD50 Oral mouse 8 5 mg kg LD50 Oral rabbit 5 mg kg LD50 Oral rat 6 mg kg RES Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL Potassium Cyanide Date 11 26 2013 PURDUE UN
300. torage area must be established for potassium cyanide and the area should be posted with a Caution Carcinogen Reproductive Toxins or Extremely Toxic Chemicals label provided by REM as shown to the right The container s should be placed into secondary containment All lab working in the lab should be made aware of the storage location and the hazards associated with potassium cyanide Potassium cyanide must only be used in a properly functioning chemical fume hood Avoid contact with skin and eyes and inhalation Avoid dust formation or breathing vapors mists or gas Keep away from acids Keep containers tightly closed Store in a cool dry and well ventilated area away from incompatible substances such as acids strong oxidizing agents iodine permanganates peroxides metallic salts chloral hydrate alkaloids and chlorates e Containers which are opened must be carefully resealed and kept upright to prevent leakage e A suitable storage location is a lab cabinet that does not contain incompatible chemicals Section 9 Spill and Accident Procedures Chemical Spill Immediately evacuate the area and ensure others are aware of the spill Dial 911 Do not allow others to re enter the affected area post a sign on the outside of the lab if necessary until emergency personnel respond If the spill could potentially harm occupants outside of the affected area i e hallway neighboring offices or labs pull the nearest fire al
301. tp www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required A face shield may also be appropriate depending on the specific application Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length flame resistant lab coats must be worn when handling volumes greater than 1 liter Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel must also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle must not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Flammable Combustible Liquids Date 7 1 2013 PURDUE UNIVERSITY Section 6 Engineering Controls Use of flammable and combustible liquids should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures
302. tructions More detail regarding sharps including biologically contaminated sharps can be found at the found in the REM Sharps and Infectious Waste Handling and Disposal Guidelines http www purdue edu rem home booklets sharps pdf 7 9 Liquid Chromatography Waste Liquid chromatography LC is an analytical technique used to separate identify quantify and purify individual components of a mixture This technique is very common in biological and chemical research The most common type of LC at Purdue is High Performance Liquid Chromatography HPLC Purdue has numerous LC instruments located in laboratories all over campus Because organic solvents e g methanol acetonitrile are commonly used in the process most LC waste is regulated by the EPA as hazardous waste Consequently all containers collecting LC waste must remain closed while the LC unit is in operation It is neither 77 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management acceptable to place a waste line running from the LC unit into an open waste container nor is it acceptable to use foil or Parafilm as a means of closure as shown in Figure 7 8 Open Waste Foil Parafilm Figure 7 8 Improper LC Waste Collection Practices One of the following practices must be employed in order to comply with hazardous waste regulations for LC waste collection systems 1 Purchase an engi
303. urchase what can be safely stored in the laboratory Avoid contact with skin and eyes Avoid inhalation of vapor or mist Avoid formation of dust Always use inside a chemical fume hood Note In case you need to dilute the concentration of acids always add acid to water Keep container upright amp tightly closed in a dry and well ventilated place Containers which are opened must be carefully resealed and kept upright to prevent leakage Keep away from sources of ignition Avoid heat and shock or friction when handling Store in original container Acids should not be stored in metal containers Keep away from incompatible materials Acids and bases should not be stored together Organic acids and Oxidizing acids must be stored separately or with proper secondary containment see below Se ni Fig 1 Demonstration of proper use of secondary containment with Organic and Oxidizing acids Use in the smallest practical quantities for the experiment being performed Work must be conducted in a chemical fume hood if the chemical is irritating to the eyes or respiratory system and or is toxic by inhalation Containers should remain closed when not in use Containers should be labeled appropriately Label should indicate the name of the chemical s in the container Avoid using chemical abbreviations acceptable if a legend is present in the lab and formulae Corrosives Date 10 1 2013 PURDUE UNIVERSITY e Containers should be in good
304. use a fire Risk of fire by shock friction or other sources of ignition May be harmful if ingested inhaled or absorbed through the skin May cause irritation to the gastrointestinal tract respiratory tract skin and eyes Section 5 Personal Protective Equipment PPE Respirator Protection Respirators should be used only under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Regulations require the use of a respirator An employer requires the use of a respirator There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended Wearing two pairs of nitrile gloves is recommended NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific flammable solid chemical being used Refer to glove selection chart from the links below http www ansellpro com downloa
305. vestigators laboratory supervisors laboratory technicians student workers and support staff have a responsibility to maintain a safe work environment All personnel working with chemicals are responsible for staying informed on the chemicals in their work areas safe work practices and SOPs and proper personal protective equipment PPE required for the safe performance of their laboratory work 1 4 1 Laboratory Supervisor Responsibilities The Laboratory Supervisor is the individual that is ultimately responsible for the overall laboratory operation including the lab safety program and ensuring that the requirements of the CHP are followed by all staff members that work in the lab For most research laboratories the Principal Investigator PI is the Laboratory Supervisor In cases where the PI has hired an individual such as a lab manager or postdoctoral scholar to manage the daily operations of the lab the PI is still ultimately responsible for the overall operation of the lab and is considered to be the Laboratory Supervisor The Laboratory Supervisor may delegate some Safety duties to a qualified individual but ultimately remains responsible for the safety of all personnel working in the laboratory Specifically the Laboratory Supervisor must e Understand applicable environmental health and safety rules including the contents of the CHP e Identify hazardous conditions or operations in the laboratory and establish SOPs and hazard asse
306. with organic compounds may cause an explosion This includes acetone photoresist isopropyl alcohol other organic solvents and nylon Figure 1 Example of vented caps Do not stockpile or accumulate Piranha solution Submit to REM for chemical waste pick up as soon as possible Piranha Solution Date 3 14 2014 PURDUE UNIVERSITY Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and begin first aid procedures Section 7 immediately Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and begin first aid procedures Section 7 immediately Seek medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste co
307. worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of pyrophoric material must be conducted in an inert atmosphere use of a glove box is recommended The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Seek medical attention immediately In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Seek medical attention immediately Lithium alkyls Date 2 1 2013 PURDUE UNIVERSITY In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least
308. xhaust The following vacuum trapping guidelines should be followed o Make sure the flask is properly clamped and secured o Make sure the vacuum hose is connected to the vacuum line not the gas line o To prevent contamination all lines leading from experimental apparatus to the vacuum source should be equipped with filtration or other trapping device as appropriate 49 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan o For particulates use filtration capable of efficiently trapping the particles in the size range being generated o For most aqueous or non volatile liquids a filter flask at room temperature is adequate to prevent liquids from getting to the vacuum source o For solvents and other volatile liquids use a cold trap of sufficient size and cold enough to condense vapors generated followed by a filter flask capable of collecting fluid that could be aspirated out of the cold trap o For highly reactive corrosive or toxic gases use a sorbent canister or scrubbing device capable of trapping the gas 5 13 Research Samples and Chemicals Developed in the Lab Research samples and chemicals developed in the lab samples must be managed responsibly Samples often accumulate in labs for years and are difficult to identify and dispose of and can create unsafe and non compliant conditions if not managed properly The following requirements apply to samples developed i
309. y issues to the department in a timely manner e Upper administrative support for safety e Self audits checklists which is a self inspection program must be completed for all areas e Abatement of deficiencies found during the self audits e Anannual safety program audit and walk through by REM and e Recommendation for ISP certification renewal from REM More information about the ISP program can be found on the REM website http www purdue edu rem home files ispinfo htm 1 7 Chemical and Laboratory Safety Committee Purdue University has established the Chemical and Laboratory Safety Committee CLSC with the responsibility to promote safe and proper chemical management at all Purdue University Campuses and related facilities Chemical management includes but is not limited to the procurement and the safe handling use storage and disposal of chemicals The CLSC reviews lab safety programs and makes recommendations to the Provost as appropriate The CLSC consists of members appointed from the faculty and staff of the major research teaching and service areas where chemicals are handled or used Although REM facilitates the content of the CHP it is ultimately the responsibility of the CLSC to approve changes and updates to the CHP 8 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Chapter 2 Chemical Classification Systems Chemical classification systems are d
310. y labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS A current copy of the SDS for Sulfuric acid must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additional lab protocol may be added here Click here to enter text NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with Sulfuric acid designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS p
311. ye Protection ANSI approved properly fitting safety glasses or chemical splash goggles Face shield is also recommended if there is a high probability of a splash hazard Skin and Body Protection Lab coats must be worn and be appropriately sized for the individual and buttoned to their full length Flame resistant lab coats must be worn if handling more than 1 liter of diethyl ether Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel must also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle must not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of diethyl ether must be conducted in properly functioning chemical fume hood The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and give oxygen If not breathing give artificial respiration Consult a physician In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing
312. zardous chemicals must be properly labeled by the chemical manufacturer or distributor before transportation occurs Chemical containers stored in laboratories are not required to be labeled per DOT standards however the DOT 9 hazard classes are often seen on chemical containers and are discussed in Section 14 of GHS formatted SDSs The DOT 9 hazard classes are illustrated below in Figure 2 5 It should be noted that Figure 2 5 only lists the primary hazard classes the sub classes e g Organic Peroxides DOT Class 5 2 were omitted for stylistic purposes ee NON FLAMMABLE GAS DOT Class 1 DOT Class 2 DOT Class 3 Explosives Compressed Gases Flammable Liquids DOT Class 4 DOT Class 5 DOT Class 6 Flammable Solids Oxidizers Poisons RADIOACTIVE A N 8 N g DOT Class 7 DOT Class 8 DOT Class 9 Radioactive Materials Corrosives Miscellaneous Figure 2 5 NFPA Hazard Rating System 15 The official version of this document will only be maintained online Chapter 2 Classes of Hazardous Chemicals Chapter 3 Classes of Hazardous Chemicals Chemicals can be divided into several different hazard classes The hazard class provides information to help determine how a chemical can be safely stored and handled Each chemical container whether supplied by a chemical manufacturer or produced in the laboratory must have a label that clearly identifies the chemical constituents In addition to a specific chemical l
313. zed for the individual and buttoned to their full length flame resistant lab coats must be worn when handling volumes greater than 1 liter Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel must also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle must not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of methyltrichlorosilane should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Methyltrichlorosilane Date 12 13 2013 PURDUE UNIVERSITY Section 7 First Aid Procedures If inhaled Move into the fresh air immediately and dial 911 If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes and dial 911 Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse In case of eye contact Check for and remove any contact lenses Rinse th

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