Model 687 Mouse Ventilator Manual
Contents
1. Plus Syringe Pump Maintenance Maintenance No special maintenance is required other than periodic lubrication of the piston O rings and the slide valve with the special non toxic grease provided To get to the slide valve for lubrication 1 Remove the four screws holding the valve to the 1 4 aluminum black back plate 2 Remove the screws in front that hold the cylinder and valve together 3 Lift entire valve assembly up and out of Pump A 5 Lubricate sparingly Model 687 Rodent Ventilator Replacement Parts c CYLINDER VALVE BLOCK 1 cc 0687 012 ASSEMBLY 0687 010 PISTON N 1 cc 0687 013 lt n p O RING 2 SCREWS 5013 051 4 40 x 1 1 8 HELICAL 5101 165 COUPLING 5108 026 Lubrication Pull stainless steel valve rod and spring assembly free of valve block 2 O RING 1 cc 5013 016 PISTON SHAFT This Model 687 Respiration Pump is shipped with Pink Grease to lubricate piston O rings in cylinders and for metal to metal applications such as the inside of the slide valve Harvard Apparatus Pico Plus Syringe Pump Maintenance This Respirator Pump is furnished with a 1 cc piston and cylinder Note It is extremely important when removing piston and cylinders that they be aligned to be concentric and in line with the piston shafi The cylinder is fastened to the 4 port vertical slide valve by means of two screws locate2. 00 300 4 ud ee t dali 11 4300 200 200 150 150 g 100 Ei H ZO E T 3 so EEE HH H LA HH so 8 60 60 a 50 Breaths per Minute 10 50 5 40 40 40 30 1 A E 1130 E fez We II A Ss 20 2 20 20 5 15 15 rai 25 E 10 60 10 3077 70 8 y a0 E 90 3 5 gt 4 100 oO 3 3 2 F 2 2 40 1 1 0 8 0 8 0 6 0 6 0 5 0 5 0 4 0 4 70 100 200 300 400 600 800 1 2 3 4 5 6 8 10 15 20 30 40 Grams Tidal Volume Kilograms Selection of Rate and Volume Refer to the Respiration Chart figure 2 to select the appropriate tidal volume and respiration rate as a function of animal body weight As a rough guide an adult rat will weigh between 200 and 500 grams with tidal volumes between 0 6 to 1 25 cm with respiration rates between 66 to 210 breaths min The average rate is 100 breaths min For most rodents the 5 cc cylinder will be adequate For larg er animals it will be necessary to change the cylinder gt Harvard Apparatus Pico Plus Syringe Pump Operating the Pump Setting Volume The volume delivered is read by observing the excursion of one of the O rings on the piston relative to the volume scale on the cylinder e g piston ring oscillates between 1 to 4 cc volume delivered is 3 cc Volume change is accomplished while the Pump is running by turning volume knob in the desired direction Setting Rate Adjust the Rate control until the desired rate appears on the LED display Avoid runni
3. EQUENTIAL DAMAGES Some states do not allow exclusion or limitation of inci dental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service depart ment The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the pur chase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practical enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not regi
4. Model 687 Mouse Ventilator User s Manual Model 687 Mouse Ventilator 55 7066 gt Harvard Apparatus Pico Plu Syringe Pump Table of Contents SUBJECT PAGE NO Table of Contents esista paie a a e aa aa EE iN 1 General Information Warranty and Repairs ee 2 SPECIHEAEIONS nee een ee 3 Description SC Features coincida 4 a 5 PumpOperationi an rca 6 8 Connecting to Animal en 6 Selecting Rate amp Volume ricino td 7 Setting Volume amp Rate sense 8 Using Gas Metureeeee runs 8 Maintenance nale lan ica gala 9 Changing Piston Gc Cylinder a rea 10 11 Harvard Apparatus Pico Plus Syringe Pump General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial num bers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to work manship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONS
5. ce Do not tighten these two screws com pletely but just enough to hold the cylinder in place The object of alignment is to have the stud at the end of the piston be exactly in line with its mating hole in the helical coupling This insures that the piston moves freely within the cylinder without undue friction The two screws at the front of the valve block control left to right alignment while the four screws at the rear of the slide valve control up and down alignment By adjustment of these 6 screws the prop er alignment can be made Remember that the screws interact with one another so the procedure may have to be repeated one or more times When proper alignment is achieved the helical coupling should be connected If alignment is correct the coupling should slide onto the piston stud without bending of the piston rod Notes L 9 dwnd 6u1l1As snig Old snyeseddy piueasiey
6. d between the room air inlet port and the to animal port on the front of the slide valve The vertical slide valve is fastened to the black 1 4 aluminum sub base by four screws locat ed on the opposite side from the printed label By using these six screws two in front and four on the side alignment adjustments are made see below Horizontal Adjustment Vertical Adjustment 5 Harvard Apparatus Pico Plus Syringe Pump Maintenance To remove the cylinder and piston 1 2 Set the pump volume to maximum Run Pump at lowest speed and stop the Pump when the helical cou pling is exposed Using a 0 050 Allen wrench provided disconnect the white piston from the coupling leaving the coupling on the piston shaft Remove the two screws located in the front of the slide valve Remove piston and cylinder Be careful to save the O ring that is recessed in the slide valve block between the screws The O ring provides the airtight seal between the plastic cylinder and the valve block To install the piston and cylinder 1 2 Pick out the new piston and cylinder Using pliers remove the piston and lubricate with the pink grease provided Insert the lubricated piston in the cylinder so that the rear of the pis ton is flush with the rear of the cylinder with the 1 8 diameter cou pling stud protruding Fasten the cylinder to the valve block with the two screws making sure the O ring is in pla
7. ed air can be collected or recycled The middle port is the room air gas channel to the animal The bottom port is the room air or gas mixture intake Note It is important that the tubes to and from the animal be as short as possible The actual connection to the animal can be either Tracheal cannula _ Endotracheal tube with or without a cuff A selection of trachael cannula and endotracheal tubes are listed in the Harvard Bioscience Catalog It is important that the volume of tubing shared by inspired and expired air dead space be kept to a minimum by having the Y or T connector as close to the animal as possible Y Connector close to animal From Animal ANIMAL gt Expired Air Tracheal EXHAUST Cannula or Endotracheal Tube To Animal TO ANIMAL OUT A A ee la Air Gas Input IN gt gt Cylinder amp Piston ROOM AIR a gt Harvard Apparatus Pico Plus Syringe Pump Operating the Pump Ventilator Graph Tidal Volume vs Weight amp Rate for Laboratory Mammals in Resting State Apparatus dead space must be added 70 100 200 300 400 600 800 1 2 3 4 5 6 8 10 15 20 30 40 500 500 400 IS HH 1 LI A 4
8. ng the Pump at rates exceeding 150 breaths min for periods in excess of 2 hours Under certain conditions this will cause the motor to become quite hot and the built in thermal overload will shut down the motor until it cools down CAUTION Do not over inflate the animal A good rule of thumb is to observe the depth of respiration in the normal calm unanesthetized animal and adjust the volume to approximate this respiration depth when the animal is attached to the ventilator Use of Gas Mixture It is possible to introduce non explosive air gas mixtures into the lower room air port However the source of gas mixture is usually from a tank delivering gas is at a constant rate while the Pump takes in air gas intermittently Some sort of buffer expansion chamber must be provided between the tank and the Pump Any kind of expandable bag such as an anesthesia bag rubber glove or Cryovac freez er bag can be used Adjust the flow from the tank so that the bag neither over inflates or collapses completely This Pump is not designed to take air gas inputs under pressure An alternate method of introducing air gas mixtures is to use a 9 to 12 ft length of open tubing connected to the input port with a T connector located close to the port Air gas is introduced into the T filling the tube when the Pump is deliver ing air gas to the animal and drawing in air gas from the tube while the animal is exhaling S Harvard Apparatus Pico
9. piration with the motion of the piston Any NON EXPLOSIVE gas air mixture can be used with the ventilator A three way slide valve is attached to the mechanism plate at the end of the cylinder and actuated by a cam on the motor THIS VENTILATOR IS NOT TO BE USED IN AN EXPLOSIVE ATMOSPHERE OR WITH ANY EXPLOSIVE GASES SUCH AS ETHER OR CYCLOPROPANE ETC THESE VENTILATORS ARE NOT EXPLOSION PROOF s Harvard Apparatus Pico Plus Syringe Pump Initial Set Up Read the Manual Model 687 is supplied with a 115 220 voltage selector slide switch located near the power cord allowing the Pump to be operated at voltages between 100 and 130 VAC or 220 to 240 VAC at 50 60 Hz Pumps are shipped with the selector switch set at 115 V For voltages other than 115V it will be necessary to cut off the existing line cord connector and install an appropriate one An international color coded line is supplied Observe the proper polarity when installing a new connector Brown high Blue neutral Green ground Turn on main power switch located on the rear panel The display will now illuminate The number displayed is the current strokes per minute rate 2 Harvard Apparatus Pico Plus Syringe Pump Operating the Pump Connection to the Animal The vertical slide valve has four ports see figure below The top port is the return channel from the animal which leaves the Pump at the port on the side It is at this top port that expir
10. stered with the FDA and is not for clinical use on human patients s Harvard Apparatus Pico Plus Syringe Pump Specifications 687 Mouse Ventilator Specifications Rate Phase Ratio Dimensions HxLxW Weight Power Stroke Rate Volume Port Size ID oD Catalog No Accessory 55 5281 Adjustable from 18 to 150 strokes min while the ventilator is running 1 1 fixed 25 x 32 5 x 15 cm 10 x 13 x 6 in 8 kg 18 Ib 115 230 VAC 50 60 Hz Continuously variable from 0 150 strokes min while the ventilator is running with LIGHT EMITTING DIODE LED DISPLAY Adjustable from 0 05 to 1 cc stroke while the ventilator is running 2 2 mm 0 086 in 4 mm 5 32 in 55 7066 Overhaul Kit for Mouse Ventilator contains O rings valve springs lubricants etc to overhaul Ventilator 3 Harvard Apparatus Pico Plus Syringe Pump Description amp Features The MODEL 687 is a positive volume ventilator intended to meet the respiratory require ments of mice weighing up to 250 g 0 55 Ib This ventilator is furnished with a 1 cc piston cylinder assemblie of 0 05 1 0 ml breath The mechanical design features a linkage that enables the piston to touch the end of the cylinder at each stroke regardless of the volume This assures that all the air gas mixture taken into the ventilator is expelled at each stroke A positive mechanical slide valve is actuated by a cam on the motor to synchronize inspiration and ex
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