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ImageQuant™ LAS 4000 - Faculté de médecine de l`université d

1. ImageQuant LAS 4000 User Manual 28 9607 42 AC 49 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr Step Action Select Sensitivity Resolution Sensitivity Resolution High Resolution Standard High Super Ultra High Binning Super Binning n R i Ultra Binning z Tip Select Help Sensitivity Resolution This opens the following dia gram that describes the relation between sensitivity and resolution Click the Start button The orange Busy LED lights on the instrument during exposure ImageQuant LAS 4000 File Edit view Option Help ImageQuant LAS 4000 Exposure Type Exposure Time Auto Manual o Sensitivity Resolution Exposure Time High Resolution v posure Time 30sec Finishing Time AM 09 40 Exposing 50 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr Action Adjust the gradations of the exposed image then save and print the image Complete Click the Complete button The display returns to the main screen Tip If the Method EtBr UV Trans illumination is used the Start button can be clicked and exposure taken even if the temperature setting state of the CCD is Not Ready 4 7 Exposing fluorescence Epi illumination This section describes how to expose fluorescent samples using Epi illumination Ensure that the cor
2. 1 Install the wide view lens See Section 3 2 Installing or exchanging the lens on page 20 80 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 15 Exposing large sample using wide view lens Action Place a sample on the tray that you selected according to the light source used Put the tray in IDX and close the door gt bmageQuant AS 4000 fe Ce Wee Often feb ImageQuant f Expe Oenberexoee Ex Ex Se Ag posure Type posure Time Ato Mns y nsitivety Resotution d Digitization image Mon t frame J ri Method Tray position Chemiluminescence Through Fluorescence EtBr hd UY Trans illumination W 605DF40 Digitization Epi illumination White Epi illumination Through Trans illumination White Trans illumination Through Tray position 105 x 70 mm Next Cancel Select the Method Light source Filter Iris and Tray position If FlatFrame Mode is set to manual in Edit Preference click the Next button Select the appropriate Flat Frame file Click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AC 81 4 Operating the ImageQuant LAS 4000 4 15 Exposing large sample using wide view lens Step Action Click the Focusing button Using for example some printed text on the sample tray turn the lens while viewing the screen and adjust the focus manually 4 Click the Retur
3. Def Returns the gradation to the initial set ting Sensitivity Resolution This function sets the sensitivity and resolution The relation between the sensitivity and resolution is shown below ImageQuant LAS 4000 User Manual 28 9607 42 AC 97 5 Software reference guide 5 7 Sensitivity Resolution Senshtivity Resolation 3840756 1924128 File size for each sensitivity High Resolution 12 6MB Standard 3 15MB High 3 15MB Super 3 15MB Ultra 3 15MB High Binning 786MB Super Binning 197MB Ultra Binning 49 2MB Tip Select Sensitivity Resolution in the Help menu The relation between the sensitivity and resolution is displayed 5 8 Method Tray position The window for setting the method and tray position is displayed when you click the Method Tray position button Select a method and tray position most suitable for the sample and click the OK button 98 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 8 Method Tray position Method Tray position 105x 70 mm h OK Cancel Set the method and tray position Method The method the combination of the optimum light source filter and iris is registered for each detection method When a method is selected the light source filter and iris are set automatically The contents of each setting are shown in the table below Note The RGB module may be used instead of the separate red green and blue Ep
4. Ub Thh driver bs not digitally signed Here ake Tell me why gimer pgreng a gertat ema yee gt cores Le lt 15 Click the Next button once again Add Haroware The wizard is mady to utall your hardware Hardware to instalt PLATAS USD Device Management Driver To start installing your new hardware click Nert ee ImageQuant LAS 4000 User Manual 28 9607 42 AC 129 6 Installing ImageQuant LAS 4000 Control Software 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 16 The following warning is displayed Proceed by clicking Install this driver software anyway W Windows Secunty lett Don t install this driver software You showld check your manufacturer s website for updated driver software for your device gt Only install driver software obtained from your manufacturer s website of dec Unsigned software from other sources may haim yout cornputer or steal infeermation iv See details E 17 Click the Finish button in the Add Hardware wizard to complete the installation Add Hardware Completing the Add Hardware Wizard The tofowing hardware was installed P FLA LAS USE Device Management Drives wast Windows has finhed mutating the software for thes device To hese thit wizard click Findh Coe Installation of the ImageQuant LAS 4000 Control Software and USB function driver Windows 7 Note During software installation
5. Light UV 312nm Filter 605DF40 Iris 2 8 Cy2 Fluorescence SYBR Blue 460nm Y515 Di 0 85 Green Epi SYBR Green Fluorescence SYBR Blue 460nm Y515 Di 0 85 Green Epi SYBR Green II Fluorescence SYBR Blue 460nm Y515 Di 0 85 Green Epi SYBR Gold Fluorescence SYBR Blue 460nm Y515 Di 0 85 Green Epi SYPRO Ruby Fluorescence SYBR Blue 460nm Y515 Di 0 85 Green Epi SYPRO Or Fluorescence SYBR Blue 460nm Y515 Di 0 85 ange Green Epi SYPROtanger Fluorescence SYBR Blue 460nm Y515 Di 0 85 ine Green Epi FITC Fluorescence SYBR Blue 460nm Y515 Di 0 85 Green Epi FAM Fluorescence SYBR Blue 460nm Y515 Di 0 85 Green Epi EGFP Fluorescence GFP Blue 460nm 510DF10 0 85 Epi ECFP Fluorescence GFP Blue 460nm 510DF10 0 85 Epi RITC Fluorescence Cy3 Green 575DF20 0 85 520nm Epi Cy3 Fluorescence Cy3 Green 575DF20 0 85 520nm Epi Cy5 Fluorescence Cy5 Red 630nm R670 0 85 Epi Alexa 633 Fluorescence Cy5 Red 630nm R670 0 85 Epi ImageQuant LAS 4000 User Manual 28 9607 42 AC Classification Reagent name Alexa 660 A Appendix A 3 Detection Reagents and corresponding settings ImageQuant LAS 4000 setting Method Fluorescence Cy5 Light Red 630nm Epi Filter Iris R670 Alexa 680 Fluorescence Cy5 Red 630nm Epi
6. Click the Focusing button Result The focusing controls appear 2 M Brightness A Adjust O O gt d oa l a PS a Confirm the sample position and the focus Click the Return button Tip Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type Exposure Type 3 v Precision Increment Repetition Program ImageQuant LAS 4000 User Manual 28 9607 42 AC 63 4 Operating the ImageQuant LAS 4000 4 9 Exposing dye stained samples and films Trans illumination Step Action Auto Exposure Type Exposure Time Auto Manual Note Automatic ex posure time setting may not be possi ble depend ing on the sample type and method 64 Select Auto or Manual for Exposure Time Manual Select an exposure time from the drop down list or enter the exposure time manually The exposure time can be set from 0 01 seconds up to 30 hours Exposure Type Exposure Time Auto Manual 10 hr Sensitivity sec St Add Digitiz O on 60min 30min 10min Smin 2min imin sec 1 2sec 1 4sec Flat frame 1 8sec Auto 1 15sec 1 30sec 1 60sec 1 100sec ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 9 Exposing dye stained samples and films Trans illumination Step Action Select Sensitivity Resolution Sensitivity Resolution High Re
7. Click the Method Tray position button Result The Method Tray position dialog opens Method Tray position Method z Fiker 105x 70 mm e Select Chemiluminescence for Method e Select Tray position according to the sample e Click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AC 41 4 Operating the ImageQuant LAS 4000 4 5 Exposing chemiluminescent samples Step Action Click the Focusing button Result The focusing controls appear Check that the sample is correctly postioned and focus the image Click the Return button Tip Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type Exposure Type Pi y Precision Exposure Increment O Auto Repetition Manu Program 42 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 5 Exposing chemiluminescent samples Action Select Auto or Manual for Exposure Time Auto Manual Select an exposure time from the drop down list Exposure Type or enter the exposure time manually The exposure Exposure Time time can be set from 0 01 seconds up to 30 hours Auto Manual Exposure Type Note Automatic Exposure Time Auto exposure Manual time setting a Sensitivity may not be ie Ba possible de Add Digitiz 2hr pending on Scone the sample oe 10min type and Smin
8. ExposureTime O Auto Manual File Name Put the appropriate tray and calibration plate in the instrument Set the exposure time Enter a file name mn fF WN N Click the Start button 5 123 Method Setting Method Setting is used to set the content displayed on the Method Tray position screen Method Setting Method List Name Light Filter Cy2 RGB p Blue Epi RGB vsi5Di Foes Cy2 E Blue Epi Blue vsi5Di Foes Cy3 RGB E Green Epi RGB 575DF20 Fo 85 cy3 E Green Epi creen 5750F20 F0 85 Cy5 RGB By Red Epi RGB rRe70 FO 85 cy5 BB Red Epi Red revo FO 85 DAPI BB vy Epi uv 4 F2 8 Digitization Epi illumination J white Epi white Through F2 8 Digitization Trans illumination oOo White Trans White O Through F2 8 v To delete a method select the method to be deleted and click Delete Note Only user defined methods can be deleted e To edit a method select the method to be edited and click Edit ImageQuant LAS 4000 User Manual 28 9607 42 AC 113 5 Software reference guide 5 12 Option menu 5 12 3 Method Setting Note Only user defined methods can be edited e To adda method click Add Result The following dialog Is displayed Method Setting TomsMethod oO Blue Epi Blue r720 FO 85 1 Enter a Name for the method 2 Select appropriate settings for Light Filter and
9. High sensitivity lens F0 85 43 mm Wide view lens F1 8 24 mm Shading correction Maximum sample size Software system 250 x 250 mm with F1 8 24 mm lens 210 x 140 mm with F0 85 43 mm lens Dynamic range 4 orders of magnitude Maximum image size 12 6 MB Light source Epi class 1 light source Blue Epi light 460 nm Green Epi light 520 nm Red Epi light 630 nm RGB Module Epi light 460 nm 520 nm or 630 nm NIR Epi light 710 nm Epi and Trans class 1M light source UV Epi light 365 NM UV transilluminator 312 nm Size 322 x 313 x87 MM UV transmitted filter size 200 x 200 mm White transmitted light table White LED Size 322 x 313 x87 mm 144 ImageQuant LAS 4000 User Manual 28 9607 42 AC A Appendix A 4 Main specifications External dimensions Camera head 224x 161 x252 mm W H D IDX 510 x 730 x 480 mm W H D Weight Camera head 3 4 kg IDX 49 kg not including the light source lens and tray High sensitivity lens 4 5 kg Power requirements Input voltage 100 240 V Voltage variation 10 Phase Single phase Power frequency 50 60 Hz Rated input current 3 0 1 5 A AC power cable Use the cable supplied with the instrument Specifications of cables required for the use of ImageQuant LAS 4000 Voltage 100 120 V Plug connector 125 V AC 13 A Cable SJT3 x 16AWG 60 C Power supply cord length Maximum 3 m Voltage 200 240 V Plug c
10. R670 Q dot Fluorescence DAPI UV 365nm Epi L41 Dy781 Fluorescence IR Infrared 710nm Epi IR785 Alexa 680 Fluorescence IR Infrared 710nm Epi IR785 Alexa 700 Fluorescence IR Infrared 710nm Epi IR785 Fluorescence dye Chemifluorescence Attophos 1 Fluorescence SYBR Green Blue 460nm Epi Y515 Di ECL Plus Fluorescence SYBR Green Blue 460nm Epi Y515 Di Digitization Silver stain Digitization Trans White Trans Through CBB stain Digitization Trans White Trans Through X ray film Digitization Trans White Trans Through NBT BCIP Digitization Ep White Epi Through 1 Attophos cannot be used for detecting nucleic acid on a nylon membrane Note A 4 With regard to patents owned by third parties related to among other things sample preparation we recommend that you consult with a lawyer or patent attorney about obtaining a license from the third parties Main specifications CCD 3 200 000 pixels Fujifilm super CCD Cooling temperature oe ImageQuant LAS 4000 User Manual 28 9607 42 AC 143 A Appendix A 4 Main specifications Number of grada tions Exposure time Recorded image 16 bit Focusing 8 bit 1 100 seconds to 2 hours Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000 Control Software
11. or Microsoft Windows Vista Business SP2 32 bit or Microsoft Windows 7 Professional 32 bit Memory 1 GB or more Processor Intel Core 2 Duo processor HD 80 GB or more USB port version USB 2 0 Optical drives DVD ROM Monitor resolution 148 1280 x 1024 pixels or more ImageQuant LAS 4000 User Manual 28 9607 42 AC A Appendix A 6 Service report fax sheet A 6 Service report fax sheet To SERVICE REPORT FAX SHEET Customer information 1 Company name 2 Person in charge 3 TEL 4 FAX 5 E mail ImageQuant LAS 4000 system information 6 Serial No e Camera head e Others 7 Analyzing unit type e Operating environment Windows e Machine model 8 Usage frequency 9 Trouble occurrence frequency 10 Error code and failure description ImageQuant LAS 4000 User Manual 28 9607 42 AC 149 For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com quantitative_imaging imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company ImageQuant are trademarks of GE Healthcare companies All third party trademarks are the property of their respective owners 2010 General Electric Company All rights reserved First published Oct 2009 All goods
12. and click the Next button Add Hareware The wizard can help you inetall other hartana The wizard can search for other hardware and automatically install it for you Or if you know exactly which hardware medel you wart to install you can select it frorn bat Whet do you wert the wizard to do Search for and install the hardware autometxaly Recommended O hratal the hardware that manually select trom a fist Advanced ek Mee gt Coren Li 7 Select Show All Devices and click the Next button Add Haroware Fron the bet below anlect the type of hardware you are inataling If you do net see the hardware category you want chck Stew All Devices Common hardwace typet SZ Onglay esesters F FLALAS 58 Device j Cg IDE ATASATAP controllers Op IEEE 1284 4 compatible printer GP TEL L284 A devices MEE 1295 Bun hoat controllers Hjima ng devices F Infrared deces ek tee gt cn ImageQuant LAS 4000 User Manual 28 9607 42 AC 127 6 Installing ImageQuant LAS 4000 Control Software 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 8 Click the Have Disk button Add Hardware Select the devica driver you wart to install for thie hardware Select the manufacturer sad model of your hardware device and then click Next you have a disk that contains the diver you want to install click Mave Duk l ee ImageQuant LAS 4000 Control Software 9 Insert the Image
13. ssessessesseesesserseeseessessess 116 61 FASCIITIS SUIS CS sensim A E ne eieeeass 116 6 2 Install ImageQuant LAS 4000 Control Software under Windows XP ecsseecseeen 116 6 3 Install ImageQuant LAS 4000 Control Software under Windows VIStO oo 120 6 4 Install ImageQuant LAS 4000 Control Software under WINCGOWS 7 wuss 124 7 Uninstalling and upgrading ImageQuant LAS 4000 Control Software 133 8 After sales service essesessesessesossessesesossessesossesoesesossessesesoesossesossesoesesessesseseses 136 SUI E N E E E E EEE AI A P E EA EE 136 8 2 REDI a A E E A O E N OT EAA 136 A PR o E E 137 EE E E EE E E AE A EA N E A A 137 A 2 Quick reference TUNEL IONS ss scssnnasatiesetstessnnvevvzonersonescanisesoideomivereconryseadedonsbiberseasvasovnnsdeadsoccesbeneueau 138 A 3 Detection Reagents ANd corresponding Settings e eecceecsesssessssessesssesssecsscssecssecsseesseesseeeses 141 AS PVCU SI ONS cca cds scnsectcse cman bccmcess son ain a cesoean ce necoreseepeeaceneeesteeecoeeseea 143 A 5 Minimum computer requirements asasnssonvuseoveensvvevsnnscrccetinsscsuverserdanmisesvavehboeeteancusidntuvigavnuniial 148 A 6 Servicereport FAX SNES suoriin aiiiar a Ea OEE ERE 149 4 ImageQuant LAS 4000 User Manual 28 9607 42 AC 1 Introduction 1 Introduction The ImageQuant LAS 4000 The ImageQuant LAS 4000 is a camera system for producing digital images of gel or membrane samples or films It is used together with the ImageQuant LAS 40
14. 2min method emiluminesc 1min sec 1 2sec 1 4sec Flat frame 1 8sec Auto 1 15sec See a 1 30sec 1 60sec 1 100sec ImageQuant LAS 4000 User Manual 28 9607 42 AC 43 4 Operating the ImageQuant LAS 4000 4 5 Exposing chemiluminescent samples Step Action Select Sensitivity Resolution Sensitivity Resolution High Resolution High Super Ultra High Binning Super Binning Ultra Binning Note The check box for Add Digitization Image can be checked to also perform a white light exposure for example to image dye stained molecular weight markers In this case the white epi light should be in place Leave unchecked if this is not necessary 5 Tip Select Help Sensitivity Resolution This opens the following dia gram that describes the relation between sensitivity and resolution Sensiivity Resolution 44 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 5 Exposing chemiluminescent samples Action Click the Start button Result The exposure is started The orange Busy LED lights on the instrument during exposure bmapeQuant LAS 4000 fe ba we Often tee ImageQuant fe ia Wew Opto Heb ImageQuant EJ Complete Click the Complete button Result The display returns to the main screen 46 Exposing fluorescent samples EtBr This section describes how to expose ethidium bromide EtBr stained samples using the UV transi
15. 4 Select Option Lens Exchange LAS 4000 ede Help CCD Calibration FlatFrame Calibration FlatFrame Calibration Pro Filter Customization m Lens Exchange Method Setting A message is displayed Lens Exchange Close the door and click OK ImageQuant LAS 4000 control software will shut down Do you want to continue Click OK Lens Exchange Switch off ImageQuant LAS 4000 instrument and change the lens Turn off the instrument using the power switch Remove the end cap from the lens Insert the lens along the lens guide with the red points facing towards you ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 2 Installing the LAS High Sens lens Red points _ 7 Connect the lens cable Turn the knurling ring until it is securely fixed L ond Ks Note Be sure to connect the lens cable Otherwise the high sensitivity lens cannot be recognized This may cause malfunction of the equipment Knurling ring Connector of the lens cable ImageQuant LAS 4000 User Manual 28 9607 42 AC 25 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 2 Installing the LAS High Sens lens 8 Remove the lens cap Lens cap CS 9 Install the lens hood 3 2 3 Removing the wide view lens 1 Close the instrument door If the power is turned off and
16. Camera cable USB cable Note Do not connect cables other than the camera cable supplied with the equipment Note Do not disconnect the camera cable other than when removing the camera head LAS High Sens lens High Sens lens cable A cable used for connecting the LAS High Sens lens and the IDX Make sure that the cable is securely connected Note e Never remove the cable except when replacing the lens e Be sure to turn off the equipment before replacing the lens Epi lights Connector on Epi light source The Epi light sources illuminate the object to be imaged from the upper right and upper left The connector is connected to the Epi light source con Figure 2 1 Incident Epi nector inside the IDX light source connector rear Epi light source connector A connector used for connecting the Epi light source Power is supplied to the Epi light source through this connector Figure 2 2 Inside the instru ment 14 ImageQuant LAS 4000 User Manual 28 9607 42 AC 2 The ImageQuant LAS 4000 2 5 Connections Note e The Epi light sources for the right and left sides are different Attach the appropriate source on respective sides e The connector will be connected when the Epi light source is inserted Make sure that the connector is securely connected See Section 3 3 2 Changing the Epi lights on page 31 e Be sure to use Epi light sources of the same type for the right and left sides
17. an F0 85 43mm LAS High Sens lens and an optional F1 8 24mm wide view lens The wide view lens can image samples up to 250 x 250 mm in size For all other applications the LAS High Sens lens is recommended This section describes how to install or exchange the lenses 20 ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 1 Removing the LAS High Sens lens FO 85 43mm LAS High Sens lens F1 8 24mm wide view lens 3 2 1 Removing the LAS High Sens lens 1 Close the instrument door If the power is turned off and the filter changer is down proceed to step 4 2 Select Option Lens Exchange LAS 4000 elaine Help CCD Calibration FlatFrame Calibration FlatFrame Calibration Pro Filter Customization m Lens Exchange Method Setting e A message is displayed Lens Exchange Close the door and click OK ImageQuant LAS 4000 control software will shut down Do you want to continue 3 Click OK 4 Lens Exchange Switch off ImageQuant LAS 4000 instrument and change the lens Turn off the instrument using the power switch ImageQuant LAS 4000 User Manual 28 9607 42 AC 21 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 1 Removing the LAS High Sens lens 5 Loosen and remove the lens hood then place the lens cap over the lens Lens hood 6 Disconne
18. and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9607 42 AC 07 2010
19. are automatically saved in a file with name format lt FileName gt _ lt PhotographNo gt _ lt ExposureTime gt e An image which is the accumulation of all exposed images is saved in a file with name format lt FileName gt _ lt Sum gt _ lt TotalExposureTime gt Note Only the 16 latest exposed images can be saved for exposure type Program ImageQuant LAS 4000 User Manual 28 9607 42 AC 95 5 Software reference guide 36 Siart After exposure type Precision Description The range can be changed by dragging the mouse Description Print image or screen Gradation can be changed by dragging the mouse Save image The gradation conversion curve can be changed linear or sigmoidal 96 Return to main screen ImageQuant LAS 4000 User Manual 28 9607 42 AC After exposure type Increment Repetition or Program For magnified image view Description The range can be changed by dragging the mouse 5 Software reference guide 5 6 Start For index image view Description Return to main screen Gradation can be changed by dragging the mouse Save image The gradation conversion curve can be changed linear or sigmoidal Print image or screen The changed gradation is applied to all images The selected image is marked by a yel low frame Click on an image to select it Double click an image or click the Image tab to open the magnified image view
20. entered in the file dex view are saved maximum 16 When the file is saved in TIFF for files mat the comment will not be saved Make Increment Image Exposure Type Program only Files of multiple exposures are accumulated and saved The range of image files to be processed in accumulation can be specified 78 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 13 Saving exposed images Action The Save dialog is displayed Type in a File name and press the Save button to save the file Save in LAS4000Image My Recent Documents My Network File name ia al Places Save as type Gel File Format gel Cancel Description Select where to save the file The file name is set automatically but may be replaced File names for single and programmed exposures are automatically set in the format lt FileName gt _n where n is the next available index For images taken in exposure type Increment file names are automat ically set in the format lt FileName gt _n_N_ lt TotalTime gt where n is the next available index N the total number of exposures and TotalTime is the total exposure time for the image Select a file format The gel format is a GE Healthcare format that records details of the exposure Such details are not saved in tif for mat Tip To automatically launch an external application to view images upon saving ref
21. images of chemiluminescent dyed or fluorescent gels and membranes The ImageQuant LAS 4000 is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices The user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of the product See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices o NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment ImageQuant LAS 4000 User Manual 28 9607 42 AC 1 Introduction 1 1 Important user information Regulations and standards supplementary information This equipment conforms to the regulations and standards described below EMC Safety Laser CE Note Note ImageQuant LAS 4000 User Manual 28 9607 42 AC VCCI Class A Conformance FCC Part 15B Class A ICES 003 Class A IEC 61326 1 2005 EN 61326 1 2006 UL61010 1 second edition CAN CSA C22 2 No 61010 1 second edition IEC6
22. reduce Coarse adjustment of focus the display The view of the focusing screen be Fine adjustment of focus comes darker The view of the focusing screen be comes lighter Tip Select Pan Focus Fast in the View menu The focusing speed then increases This is effective for the alignment of a sample see Pan Focus Fast on page 110 Note e When the NP tray is used adjust so that the bottom ofa plate is focused Adjustment using a printed paper facilitates focusing e Check the tray positions to be exposed in advance 92 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 6 Str 5 6 Start Exposure starts when you click the Start button During exposure type Precision During exposure a progress bar and ending time are displayed to indicate the progress Status of exposure ImageQuant LAS 4000 File Edit view Option Help ImageQuant LAS 4000 Exposure Type Exposure Time Auto Manual 30 sece Y Sensitivity Resolution Exposure Time High Resolution Yy p 30sec Finishing Time AM 09 40 Exposing OTT 25 C Fluorescence EtBr Flat frame Auto Click Cancel to discontinue exposure The current image Is not saved ImageQuant LAS 4000 User Manual 28 9607 42 AC 93 5 Software reference guide 36 Start During exposure type Increment or Repetition Description Description Range Scope can be changed b
23. sales Thereafter repair service may not be provided if repair parts become out of stock ImageQuant LAS 4000 User Manual 28 9607 42 AC A Appendix Appendix A Appendix A 1 Glossary CCD Calibration Electric charges are accumulated on the CCD even if it is not exposed to light The accumulated electric charge is called a dark current noise The process for eliminating the noise component is performed by CCD calibration The image constituted by the dark current noise is called CCD calibration data An image that is constituted by only the electric charges accumulated during exposure can be created by subtracting the CCD calibration data from the exposed image Flat Frame In an exposure system using a 2D sensor such as a CCD unevenness due to the lens or lighting system appears on the image By correcting the unevenness an object that radiates light at the same brightness is represented evenly in any section of an exposure area A correction file for correcting unevenness is called a flat frame Under the same conditions tray position iris fluorescent filter light source and tray as when exposing an object sample a flat frame records the image in a uniform fluorescent plate fluorescent mode or white plate digitize mode ImageQuant LAS 4000 User Manual 28 9607 42 AC 137 A Appendix A 1 Glossary Conceptual diagram of CCD calibration and flat frame Sample image Raw image Virtual profile in which light is suppo
24. 00 Control Software to e Expose chemiluminescent samples or membranes e Expose fluorescent samples using epi incident or trans transmitted light sources for example gels stained by ethidium bromide EtBr The ImageQuant LAS 4000 can be supplied with NIR Red Green Blue and UV epi lights e Expose dye stained gels and membranes or films using epi or trans illumination e Expose a sample repeatedly incrementally or by programming exposure schemes Purpose of this manual The ImageQuant LAS 4000 User Manual provides detailed instructions on using the ImageQuant LAS 4000 and outlines the functions of the ImageQuant LAS 4000 Control Software Note Be sure to refer also to the manual Getting Started with ImageQuant LAS 4000 for regulatory information specifications and the troubleshooting guide 1 1 Important user information Read this before using the ImageQuant LAS 4000 lil All users must read the safety instructions in Getting started with ImageQuant LAS 4000 before installing using or maintaining the equipment ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 1 Introduction 1 1 Important user information Do not operate the ImageQuant LAS 4000 in any other way than described in the user documentation Otherwise you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Intended use The ImageQuant LAS 4000 is a camera system that produces digital
25. 1010 1 2001 second edition IEC61010 2 081 first edition EN 61010 1 2001 EN 61010 2 081 2002 USA 21 CFR Chapter Subchapter J Part 1040 10 Laser Products IEC 60825 1 2001 LV Directive 2006 95 EC as amended EMC Directive 2004 108 EC as amended Machinery Directive 2006 42 EC This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil num rique de la classe A conforme la norme NMB 003 du Canada This is a class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures 1 Introduction 1 1 Important user information Information for traceability Manufacturer GE Healthcare Contact information See back cover Power cable 1 In Japan and North America Do not use cab
26. 607 42 AC 103 5 Software reference guide 5 10 Edit menu File Format Select the default format for saving images ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format FlatFrame Mode Launch Application Print Adjust Increment Digitization Epi illuminatiorn Timer Suto Range gel File Formati gel w e Gel File Format gel Linear 16 bit grayscale encoding Can include additional information compared to tif format e Original Image TIFF File Linear 16bit Gray tif This is a 16 bit TIFF format e Window Image TIFF File 8bit Color tif This is a TIFF format that has an eight bit color gradation The changed gradation can be saved directly 104 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 10 Edit menu FlatFrame Mode ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format FlatFrame Mode Launch Application Print Adjust Increment Digitization Epi illumination Timer Auto Range O Manual Description Auto Optimal correction data is automatically set Manual Correction data can be selected in the Flat Frame selection screen Launch Applicaton This option can be used to automatically launch an external application when an image is saved ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format FlatFrame Mode Launch Application Print A
27. 8 Method Tray position Tip e The display on the main window changes to reflect the current choice of method and tray position e The NP tray is to be used at tray position 3 using the Chemiluminescence method and the NP check box should be checked The tray will not be detected otherwise Manual flat frame mode In order to be able to manually specify a Flat frame file for the method and tray position set FlatFrame Mode to Manual in Edit Preference Click Next in the Method Tray position dialog to select the appropriate Flat Frame Correction ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format FlatFrame Mode Launch 4pplication Print Adjust Increment Digitization Epi illumination Timer Suto Range Manual Tip Only Flat Frame files that satisfy the currently selected exposure conditions are displayed in the Flat Frame Correction list To create a Flat Frame file see the description of the Flat Frame Calibration function Section 5 12 2 Flat Frame Calibration on page 112 Note Before clicking the OK button be sure to close the instrument door 5 9 File menu Page Setup Opens the settings for the printer for example the direction of paper ImageQuant LAS 4000 User Manual 28 9607 42 AC 101 5 Software reference guide 5 9 File menu Print Save Quit Outputs an image to a printer The image can be printed as a full scale print or screen print For the output method s
28. CA Installed On 6 11 2009 is NET Framework CLR Installed On 6 11 2009 fey NET Framework WinForms Installed On 6 11 2009 a v ImageQuant LAS 4000 User Manual 28 9607 42 AC 133 7 Uninstalling and upgrading ImageQuant LAS 4000 Control Software Note Correction files created during calibration are required by the ImageQuant LAS 4000 Control Software They are not deleted during the uninstallation and remain in the Data folder of ImageQuant LAS 4000 Control Software folder Uninstalling ImageQuant LAS 4000 Control Software under Windows Vista Step Action 1 2 Open the control panel and Select Uninstall a program under Programs Select ImageQuant LAS 4000 and then click Uninstall rs aip pe r msira 1 rpm e ape miraa Confirm the uninstallation by clicking Yes when prompted If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow 134 ImageQuant LAS 4000 User Manual 28 9607 42 AC 7 Uninstalling and upgrading ImageQuant LAS 4000 Control Software Uninstalling ImageQuant LAS 4000 Control Software under Windows 7 Step Action 1 Open the control panel and select Uninstall a program under Programs 2 Select ImageQuant LAS 4000 and then click Uninstall me Se a pepe ait i bee a a arad Bee ciah Lial 2 Sarge m Dagar p s i D i 3 Confirm the uninstallation by cl
29. Control Software version number Nov Wed 25 15 25 14 2009 Serial 1234567 FUJINON VRF43LMD Cooling 25C Precision Standard 1sec Chemiluminescence Tray Position 1 y Range 512 4607 Gradation 512 4607 Linear ImageQuant LAS 4000 mini 1 1 0 17 4 13 Saving exposed images The exposed image can be saved using the Save button displayed in the post exposure state or by File Save This section describes how to save an image 1 Click on the Save button or select File Save ImageQuant LAS 4000 User Manual 28 9607 42 AC 77 4 Operating the ImageQuant LAS 4000 4 13 Saving exposed images Step Action When the Save Function screen is displayed select one of the following 3 saving methods to save the photographed image Click Next button Save Function Save selected images Save displayed images Make integrated image Save passed images Up to 84 images Comment Description i Description Save Selected Image Save Images in temporary folder Exposure Type Increment Repe Max84 2 tition Program Image files not displayed in the Index view are saved in the 100 For multiple files specify the latest images maximum 84 files range of files to be saved Save Display image Max16 Comment Exposure Type Increment Repe When the file is saved in gel for tition Program mat gel a comment can be All image files displayed in the in
30. During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Action Disconnect ImageQuant LAS 4000 Control Software from the computer Open the control panel and click Classic View in the upper left corner Open Add Hardware In the Add Hardware dialog click the Next button Select Install the hardware that manually select from a list Advanced and click the Next button Select Show All Devices and click the Next button hid Himd isa Foram Ofer bri ebem yebe ee hyper al heer prr e oye ma Ai dod mbe Pa Peia jake pi eed hih er Al Sao ee jii a Tapii siiis gated ATAA TAP comin ee EEE i i cmp priar Be pE 1 i i AP CEE 1TH baio ooa Fag Seve bined i a Mieri ni iisi anl am Click the Have Disk button Insert the ImageQuant LAS 4000 Control Software DVD and click the Browse button ImageQuant LAS 4000 User Manual 28 9607 42 AC 121 6 Installing ImageQuant LAS 4000 Control Software 6 3 Install ImageQuant LAS 4000 Control Software under Windows Vista Step Action 9 Select to install the driver from the ImageQuant LAS 4000 Control Software DVD Look in DYD CD RiW Drive D ImageQuant LAS aof 6 EF fly shite aii te modif Location ay EP Network Recent Places admin Pu
31. Exchanging accessory parts 3 3 Changing the light sources 3 3 1 Changing the UV transilluminator or white light table Peak wavelength UV Epi light 365 nm Blue Epi light 460 nm Green Epi light 520 nm Red Epi light 630 nm Incident RGB module 3 color Epi light 460nm or 520nm or 630 nm NIR Epi light 710 nm White Epi light 3 3 1 Changing the UV transilluminator or white light table The UV transilluminator and white light table are exchanged as follows 1 If neither the UV transilluminator or white light table are currently in place inside the instrument proceed to step 4 2 Turn the lever holding the light source to unlock it 3 Pull out the light source carefully along the guide while holding it with both hands 4 Push the desired light source in along the guide at the bottom of the IDX 30 ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Exchanging accessory parts 3 3 Changing the light sources 3 3 1 Changing the UV transilluminator or white light table 5 Turn the lever at the front to lock the light source into position 3 3 2 Changing the Epi lights This section describes how to change a set of Epi lights The RGB module Epi lights are also installed as described here Note The tray should be at tray position 4 before installing Epi lights If the tray is higher then it should be lowered to tray position 4 using the ImageQuant LAS 4000 Control Software
32. Exposes for each interval time set in Interval Time and displays images for each section Program Exposure is made for the desired time and exposure intervals A file is created automatically for each read image and the file of accu mulated images is also saved automatically ImageQuant LAS 4000 User Manual 28 9607 42 AC 87 5 Software reference guide 5 1 Exposure type Precision Sets the exposure time _________ gt and creates one image Increment gt Sets the exposure Image 1 interval and creates multiple accumulatec C___ gt Image 2 images image 2 __3 C__i Cl_s8 gt Image 3 Repetition gt Sets the exposure Image 1 interval and creates multiple images E gt Image 2 C gt Image 3 Program O gt Dasired exposure tims Image 1 and exposure interval are set and multiple gt images are created mage Exposure Interval Walt gt preset in Image 2 Exposure interval Wait gt preset in Image 1 Exposure interval Wait mage 4 preset in Image 3 oN s 5 a gt File only Exposure interval Wait preset in Image 4 Exposure time 5 2 Exposure time This function is for setting the exposure time When exposure type is precision e Auto The exposure time is automatically calculated by histogram analysis 88 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 2 Exposure time Manual Select a suitable exposure time from the drop down list Alterna
33. Exposure Increment Auto Repetition Manus Program 58 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 8 Exposing dye stained samples and films White Epi light Action Select Auto or Manual for Exposure Time Auto Manual Select an exposure time from the drop down list Exposure Type or enter the exposure time manually The exposure Exposure Time time can be set from 0 01 seconds up to 30 hours Auto Manual Exposure Type Note Automatic Exposure Time Auto exposure Manual time setting a Sensitivity may not be ie Ba possible de Add Digitiz 2hr pending on Scone the sample oS 10min type and Smin 2min method emiluminesc 1min sec 1 2sec 1 4sec Flat frame 1 8sec Auto 1 15sec See a 1 30sec 1 60sec 1 100sec ImageQuant LAS 4000 User Manual 28 9607 42 AC 59 4 Operating the ImageQuant LAS 4000 4 8 Exposing dye stained samples and films White Epi light Action Select Sensitivity Resolution Sensitivity Resolution High Resolution Standard High Super Ultra High Binning Super Binning Ultra Binning Select Sensitivity Resolution in the Help menu You can display Help that describes the relation between sensitivity and resolution Sensiivity Resolution Click the Start button The orange Busy LED lights on the instrument and a progress dialog is displayed during exposur
34. GE Healthcare ImageQuant LAS 4000 User Manual Table of Contents Table of Contents E 3 5 Ge ed 0 9 eee ene ee ae ee ee enn oe ee anne ror ert reams er anes mer ene ter IL Imponan TS WE ORT II sssr A E eases stan 5 2 The ImageQuant LAS 4000 escsssssscssecssccssesssscsseesseesscssssesssecsseesecesseees 11 2 1 The IMAGEQUANT LAS 4000 exterior oaeeeeccecsesssessssssssesssecssecsscssscsssesssessseessecssecssecssecsssesseessneessees 11 22 nsid thelmageQuant LAS SOOO wiiren eaa ri 12 Cae E E E 1 ET E N E EA E T E E AE 13 2 4 Parts and dCCESSONES sssini nea E EEEa TRENU Ea E area a aanse 16 3 Exchanging dccess ry NOS soucssrsrinniiiarpenoiniana 19 Sel Changing or installing a filter sscsscescucvvxcscssscrscstioestvasnascondiscdecpseebesiesspossedeesiastuslesossntserprenactiioennesrabtbons 19 32 installing OF exchanging the IENS ceases cdsescivenssustacassictsesclebspussicssbeantedssnecosseoyendcteastecendisuncdceeeesees 20 3 2 1 Removing the LAS High Sens lens ssssssssssssssssssssssssssssssssssssssssssssssssssssrereserrserrerrerrrrrrrerreerns 21 3 2 2 Installing the LAS High Sens lens cas secscstussxtcccserstcesusscesseaststescordunshetdsasescesastestteaseestiitaieccseadi seeks 24 3 2 3 Removing the wide view lens sssssssssssssssssssssssssssssssssssssssssssssssssssrssrsrersrrssrrrrrrrrrrrrrrrrrrrns 26 3 2 4 Installing the wide view lens ssesssssssssssssssssssssssssssssssssssssssssssssss
35. Imin sec 1 2sec 1 4sec Flat frame 1 8sec Auto 1 15sec 1 30sec 1 60sec 1 100sec ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 7 Exposing fluorescence Epi illumination Action Select Sensitivity Resolution Sensitivity Resolution High Resolution Standard High Super Ultra High Binning Super Binning Ultra Binning Select Sensitivity Resolution in the Help menu You can display Help that describes the relation between sensitivity and resolution Click the Start button The orange Busy LED lights on the instrument and a progress dialog is displayed during exposure Adjust the gradations of the exposed image then save and print the image Click the Complete button The display returns to the main screen ImageQuant LAS 4000 User Manual 28 9607 42 AC 55 4 Operating the ImageQuant LAS 4000 4 8 Exposing dye stained samples and films White Epi light 48 Exposing dye stained samples and films White Epi light This section describes how to expose dye stained samples using white Epi illumination For some applications such as imaging gels stained with Coomassie Blue dye results may not be optimal using the white epi light If the White trans tray is available see Section 4 9 Exposing dye stained samples and films Trans illumination on page 61 for a more optimal method Place a sample on the Epi tray Put the tray in t
36. Iris 3 Click OK Note Optical characteristics can be corrected using the Flat Frame Calibration function for the added Method See Section 5 12 2 Flat Frame Calibration on page 112 5 13 Help menu User Manual Opens the ImageQuant LAS 4000 User Manual in the default pdf browser Getting Started Opens the Getting Started with ImageQuant LAS 4000 manual in the default pdf browser End User License Agreement Opens the End User License Agreement in the default pdf browser 114 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 13 Help menu Sensitivity Resolution The figure showing the relation between sensitivity and resolution is displayed Click to close the window Sensitivity Resolution About ImageQuant LAS 4000 Control Software The version information of Image Reader is displayed Click to close the window E a LAS 5 4000 o a Version 1 1 i Build 1 1 0 17 Instrument SIN 9678001 TROL ZQETWA ky PANY ALL RIGHTS RESERVED pa ak cal S support elifesciences comjcontact ImageQuant LAS 4000 User Manual 28 9607 42 AC 115 6 Installing ImageQuant LAS 4000 Control Software 6 Installing ImageQuant LAS 4000 Control Software Administrator priveleges required All tasks related to software installation or uninstallation require a computer account with administrator priveleges 6 1 Installation sequence Software installation is performed in the followi
37. Quant LAS 4000 Control Software DVD and click the Browse button Insert the manufacturer s installation disk and then make sure that the correct drive is selected below Copy manufacturer s files from Lask p E iyt ai E a 7 fe By Hard Derk Drives 2 a l Local Oreck C aS Es ae 0D tree of a z HP_ RECOVERY Th oP SF scien E Devices wiih Removable Stomge 4 Libraries i Flappy Dok Dine 5 LS DVDSCD Fivt Derve i D bytes tree pi XI hee pemeri 11 Select the USB Control folder and click the Open button 128 ImageQuant LAS 4000 User Manual 28 9607 42 AC 6 Installing ImageQuant LAS 4000 Control Software 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 12 Select the file DevMng and click the Open button fy Locate Fie Laske ji USB Contras 677 Heme 5 Date modded Type mi Files Currentiy on tha Dise 0 Recent Pieca 6 nevidng LEA 2MO 3M PM Setup Inf Desktop Libraries iE Ri i Hege ving oa Flea ef ppe Getuo iforrabsn fant Caneel 13 Click the OK button in the Install from disk dialog 14 Click the Next button in the Add Hardware wizard Add Hardware Select the devica driver you wart to install for thie hardware Select the manufacturer and model of your hardware device and then click Next F you have a disk that contains the driver you want to install click Mave Duk Medel FUV LAS USE Device Management Oriwer
38. S 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 11 Exposing repeatedly Repetition Step Action Click the Focusing button Result The focusing controls appear fe C2 Vew Opto feb ImageQuant Exposure Type Repeto Repeat Lirit m 100 gt a ky Confirm the sample position and focus Click the Return button Tip Click on the image to magnify it Click on the image again to return to the original size Select Repetition for Exposure Type Exposure Type 3 Re Precision Increment Repetition Manus Program 10 sec Y Interval Tin Select Interval Time unit and enter a numeric value Interval Time Manual 10 U w 4 Sensitivity Res Stender MES Repeat Limit C Set Limit ii Note Interval Time can be entered in the range of 10 seconds to 2 hours ImageQuant LAS 4000 User Manual 28 9607 42 AC 13 4 Operating the ImageQuant LAS 4000 4 11 Exposing repeatedly Repetition Step Action Select Sensitivity Resolution Sensitivity Resolution High Resolution Standard High Super Ultra High Binning Super Binning Ultra Binning Tip Select Sensitivity Resolution in the Help menu You can display Help that describes the relation between sensitivity and resolution gt SensitivityiResolut Smrovtting To specify the maximum number of exposures to be taken check the Set Limit ch
39. al 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 4 Placing the sample 3 Choose the exposure size and tray position For Epi and Trans trays e The readable area varies depending on the tray position Place the sample in position according to its size 250mm X 250mm 210mm X 140mm 180mm X 120mm 144mm X 96mm 105mm X 70mm Position Position i Position Position Maximum capture size when a wide view lens is used Tip On the Epi tray there are round dents marked on the tray for positioning the sample Line up the sample using the appropriate dents For NP tray e The NP tray is to be used at tray position 3 Note The readable area will be slightly smaller using the F1 8 wide view lens ImageQuant LAS 4000 User Manual 28 9607 42 AC 39 4 Operating the ImageQuant LAS 4000 4 4 Placing the sample 4 Open the IDX door and insert the tray Place the Epi or Trans trays in position with the side with a hole facing outwards 5 Ensure the lens cap is removed and close the IDX door 45 Exposing chemiluminescent samples This section describes how to take images of chemiluminescent samples No illumination is used for this application Place a sample on the Epi tray Put the tray in the ImageQuant LAS 4000 then close the door 40 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 5 Exposing chemiluminescent samples Action
40. ator password if prompted then click Continue to proceed with the installation 1 Disconnect ProductNameHW from the computer ImageQuant LAS 4000 User Manual 28 9607 42 AC 125 6 Installing ImageQuant LAS 4000 Control Software 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 2 Open the control panel and click Hardware and Sound faded your CO a a e Parth kad Seed ity a Farse pe PAB i PA bei op pp eee Ba riet gt ra tea Ch 3 Click Device Manager under Devices and Printers oe TP ecm 00h i LIT P on bee aj beam riea eed peren iain ee ra o f Ues e a 126 ImageQuant LAS 4000 User Manual 28 9607 42 AC 6 Installing ImageQuant LAS 4000 Control Software 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 5 Inthe Add Hardware dialog click the Next button Add Hareware Welcome to the Add Hardware Wizard Tha wuaed helps you instali daver software to support older devices thet do net support Plug and Pley end winch ere not sutormatxcally recogneed by Windows You should oey use tha waard if you ate an advanced user or you have been deected here by technical support a If your hardware tame with an installation CD it is recommended that you click Cancel to clove this wizard and wee the manufacturer s CD to instal this hardware To cennus cick Newt Met gt Carcat ke a 6 Select Install the hardware that I manually select from a list Advanced
41. before attaching the lights as described below Latch Epi light base 1 If the tray is at Tray position 4 proceed to step 3 Otherwise close the IDX door 2 Click on Method Tray position set Tray position to 4 and click the OK button 3 Open the IDX door If no Epi light is currently in place inside the instrument proceed to step 7 4 Spring up the latch to unlock the Epi light ImageQuant LAS 4000 User Manual 28 9607 42 AC 31 3 Exchanging accessory parts 3 3 Changing the light sources 3 3 2 Changing the Epi lights 5 Holding the latch with one hand remove the Epi light by pulling it gently outwards 7 Before installing the new Epi light ensure that the latch is sprung open upwards 8 Hook the hanger located on top of the Epi light to the top edge of the Epi light base Two guide holes are located on the back of the Epi light base Slide the hook after attaching the guide pins to the guide holes 32 ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Exchanging accessory parts 3 3 Changing the light sources 3 3 2 Changing the Epi lights 9 Push the Epi light while holding the latch open and slide it inwards until a click sound is heard 10 Lower the latch to lock the Epi light in place A FAN yA ae J 11 Repeat this process for the opposite side Note e The Epi lights are clearly labelled L for the left and R for the right sides Install the Epi
42. blic jE Computer Floppy Disk Drive amp Ei Disk C Windows do System32 DYD CD R W Drive D ImageQuant LAS 401 a STORE N GO E File name inf Files of type Setup Information inf 10 Lookin 3 DYD CD RW Drive D ImageQuant LAS 401 v 6 F E fly Name Size Type Date modif Location Files Currently on the Disc 2 Recent Places USB Control File Folder Dekap Ji USB Function ij File Folder admin File name inf Files of type Setup Information inf 11 Select the file DevMng and click the Open button 12 Click the OK button in the dialog Install from disk 122 ImageQuant LAS 4000 User Manual 28 9607 42 AC Step 6 Installing ImageQuant LAS 4000 Control Software 6 3 Install ImageQuant LAS 4000 Control Software under Windows Vista Action 13 14 19 16 Click the Next button in the wizard Add hardware Click the Next button once again The following warning is displayed Proceed by cliking Install this driver software anyway E inaen ay Dont ratal this dr T Bolte ial ihis drier weltware ampay i o ba drah Click the Finish button in the Add Hardware wizard to complete the instal lation Install ImageQuant LAS 4000 Control Software and USB function driver Windows Vista Note Step During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator passwo
43. can be kept Previous images are discarded Printing exposed images The exposed image can be output as a full scale print or screen print the window displayed on the screen The print can be done using the Print button displayed in the post exposure state or the File Print This section describes how to print an image Action For a screen print first display the window you wish to output Click the Print button or select Print from the File menu 76 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 12 Printing exposed images Action Select the output setting for the printer and the type full scale print paper size print or screen print Click the OK button Full scale print stunt ser Paper size print C tary Pies Screen print The Print dialog is displayed Choose settings for the printer and click the Print button The image is printed Tip Exposure information is printed together with the image except for the Easy Print option This is convenient since it is unnecessary to note the exposure information and the conditions The following exposure information is included Exposure date camera serial number lens type cooling temperature at the time of exposure exposure type sensitivity exposure time or interval time name of Method tray position number values at both ends of the range scope values at both ends of the gradation type of gradation
44. cision 5 11 View menu Paint saturated data red This function toggles whether to display saturated data in red Negative Gray This sets the display color of an image such that absorbing bands are darker and the background lighter ImageQuant LAS 4000 User Manual 28 9607 42 AC 109 5 Software reference guide 5 11 View menu Positive Gray This sets the display color of an image such that absorbing bands are lighter and the background darker Red This sets the display color of an image such that illuminated regions are displayed in red Green This sets the display color of an image such that illuminated regions are displayed in green Blue This sets the display color of an image such that illuminated regions are displayed in blue Pan Focus All This function toggles the quality of a focus image The image is displayed in high image quality when this option is selected Pan Focus Fast This function toggles the quality of a focus image With this option selected the image quality is lower than using Pan Focus All but it is displayed faster Tip During sample alignment a sample can be quickly aligned when Pan Focus Fast is selected 110 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 12 Option menu 5 12 Option menu 5121 CCD Calibration This function creates a correction file that is used for correcting characteristics of the CCD detector The creat
45. ct the lens cable connector by rotating the knurling ring VA sn O H NL 7 Turn the lens slightly to the left and loosen it while pushing the lens release lever Connector of the lens cable 22 ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 1 Removing the LAS High Sens lens 8 Rotate the lens to the left by 45 degrees The lens comes off downward Note Hold the lens with both hands so as to prevent the lens guide from being damaged 9 Take out the lens slowly along the lens guide toward you 10 Place the end cap on ImageQuant LAS 4000 User Manual 28 9607 42 AC 23 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 1 Removing the LAS High Sens lens 5 2 2 24 Note e Be sure to put the high sensitivity lens sideways and lower it gently when placing it on the desk Placing the lens downward may affect the mechanical precision of the lens barrel e Make sure that the filter changer has come down far enough to make room for replacing the lens e Do not let the high sensitivity lens hang on the lens guide The lens may fall causing injury or damage e The high sensitivity lens weighs 4 5 kg Be careful when handling it Installing the LAS High Sens lens 1 Close the instrument door lf the power is turned off and the filter changer is down proceed to step
46. djust Increment Digitization Epi illumination Timer Auto Range C Activate save and launch function To define an application to launch do the following ImageQuant LAS 4000 User Manual 28 9607 42 AC 105 5 Software reference guide 5 10 Edit menu e Select the application to be launched from the drop down list or locate the application using the Select button e Check the Activate save and launch function checkbox Print Adjust Adjusts the print output size ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format FlatFrame Mode Launch Application Print Adjust Increment Digitization Epi illumination Timer Auto Range Width 100 0 te Height 100 0 So 106 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 10 Edit menu Increment ImageQuant LAS 4000 Preference OCD Cooling Root Folder File Format FlatFrame Mode Launch Application Print Adjust Digitization Epi illuminatian Timer Autoa Range Caa Description During incremental imaging exposure is automatically stopped when the amount of saturated data is too large During incremental imaging exposure is continued until the Stop button is pressed ImageQuant LAS 4000 User Manual 28 9607 42 AC 107 5 Software reference guide 5 10 Edit menu Digitization EPI ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format F
47. dule Epi lights set of two NIR Epi light 710 nm set of two Filters Y515 filter Y515 GFP detection filter 510DF10 GFP EtBr detection filter 605DF40 EtBr Cy3 detection filter 575DF20 Cy3 Cy5 detection filter R670BP Cy5 Alexa Fluor 750 detection filter IR785 Alexa Ultraviolet protection filter L41 UV ImageQuant LAS 4000 User Manual 28 9607 42 AC 17 2 The ImageQuant LAS 4000 2 4 Parts and accessories Note e The combined accessories and light source vary depending on the system that you purchase e Make sure to retain the caps and filter cases e Store the incident Epi light sources in a container box e The UV trans tray will degrade after many UV illuminations It can be used up to approximately 1000 times if each exposure time is 1 second New trays can be purchased separately 18 ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Exchanging accessory parts 3 Exchanging accessory parts 3 1 Changing or installing a filter Optical filters are installed in the filter turret localted under the lens inside the IDX This section describes how to install or change a filter on the filter turret Install a filter in the filter turrent and register it in the ImageQuant LAS 4000 Control Software as follows Ei ImageQuant LAS 4000 File Edit view Option Help ImageQuan 3 LAS 4000 R i an a 6 0 ap l Change Filter and Filter posi
48. e Adjust the gradations of the exposed image then save and print the image Wa BE Wm pen Hi ImageQuont LAS 6000 Click the Complete button The display returns to the main screen 60 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 9 Exposing dye stained samples and films Trans illumination 49 Exposing dye stained samples and films Trans illumination This section describes how to expose dye stained samples using white trans illumination Place a sample on the White trans tray Put the tray in the IDX and close the door ImageQuant LAS 4000 User Manual 28 9607 42 AC 61 4 Operating the ImageQuant LAS 4000 4 9 Exposing dye stained samples and films Trans illumination Step Action Click the Method Tray position button Result The Method Tray position dialog opens Method Tray position Method Filter Chemiluminescence Fluorescence UY Trans illumination Y 605DF40 Digitization Epi illumination White Epi illumination Through Trans illumination White Trans illumination Through Tray position 105x 70 mm OK Cancel 1 Select Digitization Trans illumination for Method 2 Select Tray position according to the sample used 3 Click the OK button 62 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 9 Exposing dye stained samples and films Trans illumination Step Action
49. e Help menu You can display Help that describes the relation between sensitivity and resolution 5 i To specify the maximum number of exposures to be taken check the Set Limit check box and specify the number of images 6 Repeat Limit V Set Limit times 1 100 ImageQuant LAS 4000 User Manual 28 9607 42 AC 69 4 Operating the ImageQuant LAS 4000 4 10 Exposing consecutively Increment 70 Step Action Click the Start button to begin exposure The index view and expanded view can be switched by clicking the Index or Image tab Note The displayed expanded image is the latest image Index view Image view ji E EE pal Acetyl ja eset A Exposure automatically stops after the number of images set in Repeat Limit Note Clicking the Stop button cancels the current exposure Tip It is possible to stop exposures automatically when the image is saturated This function is activated in Edit Preference under the Increment tab ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 10 Exposing consecutively Increment Action During exposure the gradation conversion curve can be changed and the gradation adjusted When the Apply All button is clicked the adjusted gradation will be applied to all images i Apply All 7 Reset All Description No Description The range can be The changed gradation changed by dragging is applied to all images the mouse T
50. ebsite of software from other sourc h your co indermation Click the Finish button The installation of ImageQuant LAS 4000 Control Software is now completed Start the ImageQuant LAS 4000 Control Software This will ensure that nec essary folders and files are created 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 Note 124 Log in using a Windows account with administrator privileges before attempting to install the software ImageQuant LAS 4000 User Manual 28 9607 42 AC 6 Installing ImageQuant LAS 4000 Control Software 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 Installation is performed in the following sequence 1 Disable the computer sleep function 2 Installation of the USB Control driver 3 Installation of the ImageQuant LAS 4000 Control Software and USB function driver Disable the computer sleep function In order to prevent scanning errors the computer should be set to never sleep as described below 1 Open the Control Panel and click System and Security Click Change when the computer sleeps below Power Options 2 Select Never in the drop down menu by Put the computer to sleep then click Save changes ar nama eF OEE Installation of the USB Control Driver Windows 7 Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administr
51. eck box and specify the number of images Repeat Limit Z Set Limit times 1 100 74 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 11 Exposing repeatedly Repetition Action Click the Start button to begin exposure The index view and expanded view can be switched by clicking the Index or Image tab Note The displayed expanded image is the latest image Index view Image view i gt T Ww Exposure automatically stops after the number of images set in Repeat Limit Note Clicking the Stop button cancels the current exposure ImageQuant LAS 4000 User Manual 28 9607 42 AC 75 4 Operating the ImageQuant LAS 4000 4 11 Exposing repeatedly Repetition Action During exposure the gradation conversion curve can be changed and the gradation adjusted When the Apply All button is clicked the adjusted gradation will be applied to all images Apply All a Reset All Description No Description The range can be The changed gradation changed by dragging is applied to all images the mouse The gradation conver Return to initial setting sion curve can be tog gled between Linear or Sigmoid The gradation can be changed by dragging the mouse 4 12 Adjust the gradations of the exposed image then save and print the image Click the Complete button The display returns to the initial screen Note Only the last 100 images
52. ed correction file varies depending on the sensitivity exposure time short or long and CCD cooling temperature 1 When CCD Calibration is selected in the Option menu the following window is shown 5 3 1 4 CCD Calibration Currant Temperature High Resolution Ready Thu Jul 2 14 50 16 2009 Ready Standard Thu Jul 2 14 51 31 2009 Ready High Thu Jul 2 14 52 31 2009 Ready Super Thu Jul 2 14 53 31 2009 Ready Ultra O Thu Jul 2 14 54 31 2009 Total Time For Calibration 0 minutes Description Description Click here to check the boxes Click here to check the boxes for all resolutions for up to 2 for all resolutions for more hours than 2 hours The time required for creation Click on the buttons in this is displayed column to check the boxes for both correction files for specif ic resolutions Check individual check boxes to generate specific correction files 2 Click the Start button to generate the file e The progress of file creation is shown on the progress bar e To stop file creation click the Stop button The file will not be saved ImageQuant LAS 4000 User Manual 28 9607 42 AC 111 5 Software reference guide 5 12 Option menu 5 12 2 Flat Frame Calibration 5122 Flat Frame Calibration This function creates a correction file Flat Frame that is required for correcting the optical characteristics The Flat Frame file varies depending on Light Filter Iris and Tra
53. ee Section 4 12 Printing exposed images on page 76 Saves a file The default setting for the file format to be saved is that selected in Preference in the Edit menu See Section 5 10 Edit menu on page 102 Exits ImageQuant LAS 4000 Control Software 5 10 Edit menu Cut Copy Paste 102 Cuts the selected characters This function can be used during manual input of exposure time and interval Copies the selected characters This function can be used during manual input of exposure time and interval Pastes the copied characters This function can be used during manual input of exposure time and interval ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 10 Edit menu Preference The available settings can be viewed by clicking the respective item CCD Cooling Sets the CCD temperature ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format FlatFrame Mode Launch Application Print Adjust Increment Digitization Epi illumination Timer CCD Cooling Temperature Auto Range Cancel Root Folder Sets the folder in which files are saved ImageQuant LAS 4000 Preference OCD Cooling Rook Folder File Format FlatFrame Mode Launch Application Print Adjust Increment Digitization Epi illumination Timer Auto Range C Documents and Settings TE te ImageQuant LAS 4000 Image ImageQuant LAS 4000 User Manual 28 9
54. elect the Tray position e Click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AC 47 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr Step Action Click the Focusing button Result The focusing controls appear 3G Brightness QT BE Adjust atte ea aan i z Ta i E l is ti H H F i i n N Confirm the sample position and focus Click the Return button Tip Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type Exposure Type 4 v Precision Exposure Increment O Auto Repetition Manus Program _ 48 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr Action Select Auto or Manual for Exposure Time Auto Manual Select an exposure time from the drop down list Exposure Type or enter the exposure time manually The exposure Exposure Time time can be set from 0 01 seconds up to 30 hours Auto Manual Exposure Type Note Automatic Exposure Time Auto exposure Manual time setting a Sensitivity may not be ie Ba possible de Add Digitiz 2hr pending on Scone the sample oe 10min type and Smin 2min method emiluminesc 1min sec 1 2sec 1 4sec Flat frame 1 8sec Auto 1 15sec See a 1 30sec 1 60sec 1 100sec
55. er to Launch Applicaton on page 105 Note Special characters such as or other characters that have a special meaning in Windows should not be used in a file name ImageQuant LAS 4000 User Manual 28 9607 42 AC 19 4 Operating the ImageQuant LAS 4000 4 14 Ending the session 414 Ending the session This section describes how to end the current session with the ImageQuant LAS 4000 The instrument should be switched off as described below when it will not be used for some time Operation Select Quit from the File menu ImageQuant LAS 4000 ES Edit view Option Help Page Setup nin Print Ttrl P Ni Quit Progra v Select Stop the CCD cooling now and click OK Stop the CCD cooling now Keep the CCD cooling after quit When Keep the CCD cooling after quit is selected the cooling tem perature of the CCD will be maintained and the instrument can be used straight away the next time the ImageQuant LAS 4000 Control Software is started In this case do not turn off the ImageQuant LAS 4000 However if the instrument will not be used for a long period it should be turned off Shut down the computer Switch the ImageQuant LAS 4000 off using the power switch 415 Exposing large sample using wide view lens A larger sample of up to 250 x 250 mm can be exposed by replacing a high sensitivity lens with a wide view lens This section describes how to expose large samples
56. ew Positive Gray Select and check Creating CCD Calibration file Option CCD Calibration Select and set conditions for Start Creating Flat Frame Option Flat Frame Calibra tion Select and set conditions for Start Checking software ver sion information Help About ImageQuant LAS 4000 Control Software Select ImageQuant LAS 4000 User Manual 28 9607 42 AC A Appendix A 2 Quick reference functions Function Function name Set item Checking the relation be Help Sensitivity Resolution Select tween sensitivity and resolution A 3 Detection Reagents and corresponding settings Note The RGB module may be used instead of the separate red green and blue Epi lights ImageQuant LAS 4000 setting Classification areal ati Method Light Filter Iris ECL Chemiluminescence Through ECL Plus Chemiluminescence Through Lumi Light Chemiluminescence Through Plus Chemiluminescence Renaissance Chemiluminescence Through Super Signal Chemiluminescence none Through 0 85 Bright Star Chemiluminescence none Through 0 85 CDP Star Chemiluminescence none Through 0 85 CSPD Chemiluminescence none Through 0 85 ImageQuant LAS 4000 User Manual 28 9607 42 AC 141 A Appendix A 3 Detection Reagents and corresponding settings Classification Reagent name EtBr ImageQuant LAS 4000 setting Method Fluorescence dye 142 Fluorescence EtBr Trans
57. exposure intervals This is the time from the shutter closure until the start of the next exposure Note The wait time can be set within the range of O seconds to 30 hours Close the settings window Set exposure time Note This can be set within the range of 0 01 seconds to 30 hours Enter a file name to be saved Save the template Enter a comment Settings will return to the initial settings Folder in which the template is saved Delete a template 1z Select the number of frames between 1 and 16 to be preset Select detection sensitivity Navigate to the folder chosen in step 6 above and delete the template file The default locations are as follows e For Windows XP C Document and Settings All users Application Data GE Healthcare lmageQuant LAS 4000 ProgramTemplate e For Windows Vista C GE Healthcare lmageQuant LAS 4000 ProgramTemplate ImageQuant LAS 4000 User Manual 28 9607 42 AC 91 5 Software reference guide 5 5 Focusing 5 5 Focusing The Brightness function enables adjustment of the brightness of the Focusing screen and the Adjust function enables adjustment of the focus when the LAS High Sens lens is used i inkpouant LAS 4000 Fin Edt View Option Heip ImageQuant LAS 6000 Exposuine Type Exposure Time Auto Matual Cm e Sensitivity Resolution REALY Me sd VIIa Description Click on the image to expand or
58. ght source into the ImageQuant LAS 4000 Light source Calibration plate Red Epi light Cal plate GR pink Green Epi light Cal plate GR pink Blue Epi light Cal plate FL green RGB module Use the Cal plate that corresponds to the chosen wavelength red green or blue NIR Epi light Cal plate GR pink UV Epi light Cal plate FL green White Epi light Cal plate DI white Close the door of the instrument Note Ensure the the appropriate lights and filter are in place To perform a Flat Frame calibration select FlatFrame Calibration in the Option menu The FlatFrame Calibration dialog opens Note Administrator priveleges are required in order to perform a Flat Frame calibration Select the method to calibrate from the drop down list Choose which tray positions to calibrate then click Start to begin the calibration Once the calibration is complete click the Close button to close the dialog The calibra tion plate can now be removed 86 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 Software reference guide 5 1 Exposure type This function is for selecting exposure methods There are four types of exposure methods Exposure Description Type Precision Exposes for the entire time set in Exposure Time Increment Exposes for each time set in Interval Time and accumulates images Repetition
59. he ImageQuant LAS 4000 then close the door Note e Prior to exposure click on the Digitization Epi illumi nation tab in Edit Prefer ence and make sure that YES is selected for Invert Pixel data If this is set to NO the data will not be in verted and the quantitative value will be reversed a peQuary fe th wee Op Heb ImageQuant lt a Ay A F A 4 ri posure Type osure Time Ato Mns naitivety Resodution d Digitization image Mon t frarne 5 56 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 8 Exposing dye stained samples and films White Epi light Action Click the Method Tray position button Result The Method Tray position dialog opens Method Tray position 144x 96 mm OK Cancel e Select Digitization and Epi illumination for Method e Select Tray position according to the sample used e Click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AC 57 4 Operating the ImageQuant LAS 4000 4 8 Exposing dye stained samples and films White Epi light Step Action Click the Focusing button Result The focusing controls appear ImageQuant LAS 6000 Confirm the sample position and focus Click the Return button Tip Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type Exposure Type 4 v Precision
60. he gradation conver Return to initial setting sion curve can be tog gled between Linear or Sigmoid The gradation can be changed by dragging the mouse Adjust the gradations of the exposed image s as described above then save and print the imagels Click the Complete button The display returns to the initial screen Note Only the last 100 images can be kept Previous images are discarded 411 Exposing repeatedly Repetition The Repetition exposure type enables a series of images to be exposed consecutively Each image is recorded separately and data is not accumulated This section describes how to perform this type of experiment Place a sample on the tray that you selected according to the type of a sample Put the tray in the ImageQuant LAS 4000 then close the door Note e Make sure that the tray is placed at the required position ImageQuant LAS 4000 User Manual 28 9607 42 AC 71 4 Operating the ImageQuant LAS 4000 4 11 Exposing repeatedly Repetition Bi Yes Open Heb ImageQuant Action Click the Method Tray position button Result The Method Tray position dialog is opened Method Tray position Chemiluminescence None Through Puoesxeme y515 Di Oigtizabon D Epbiuminstion White Epi fuminstion Trans fumination White Trans diennation 105x 70 mm OK Cancel Select the Method and Tray Position Click the OK button 72 ImageQuant LA
61. hrough FO 05 Muorexeme Y515 Di O Cigtizebon 3 Ephiuminsion White Epiuminstion Trans fumination White Trans dianination 105x 70 mm OK Cancel Select the Method and Tray Position Click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AC 67 4 Operating the ImageQuant LAS 4000 4 10 Exposing consecutively Increment Step Action Click the Focusing button Result The focusing controls appear ImageQuant LAS 4000 Expouurn Type C Interval Time Seratilvity Resolyiken Rapea Lirit Te et _ ise tires 1 100 2 Confirm the sample position and focus Click the Return button Tip Click on the image to magnify it Click on the image again to return to the original size Select Increment for Exposure Type Exposure Type a een Interval Ti EE Repetition Manu Program 10 Select Interval Time unit and enter a numeric value Interval Time Manual 10 hr 4 Sensitivity Res aa Standar Repeat Limit C Set Limit Note Interval Time can be entered in the range of 10 seconds to 2 hours 68 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 10 Exposing consecutively Increment Step Action Select Sensitivity Resolution Sensitivity Resolution v High Resolution Standard High Super Ultra High Binning Super Binning Ultra Binning Tip Select Sensitivity Resolution in th
62. i lights Method Filter Iris Chemiluminescence None Through SYBR Green Blue 460nm Epi Y515 Di GFP Blue 460nm Epi 510DF10 UV 312nmTrans il 605DF40 lumination Fluorescence Blue 460nm Epi Y515 Di Green 520nm Epi 575DF20 Red 630nm Epi NIR 710nm Epi UV 365nm Epi ImageQuant LAS 4000 User Manual 28 9607 42 AC 99 5 Software reference guide 5 8 Method Tray position Method Filter Iris Digitization Epi illumina White Epi illumina Through tion tion Trans illumina White Trans illumi Through tion nation Note The method that can be used varies depending on the state of the light source set in IDX Unusable options cannot be selected Note Before clicking the OK button be sure to close the IDX door Note Filter position 1 is dedicated for chemiluminescence Do not put a filter in it Tray position The tray position is changed according to the sample size The tray size that can be read varies depending on the tray position Position Width mm Length mm 105 70 144 96 180 120 210 140 250 wide view lens 250 wide view lens cf 200mm 250mm S SS A 210mm 140mm O 05mm 7Omm Hl E Maximum exposure size when a wide view lens is used 100 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5
63. icking Yes when prompted 4 If User Account Control UAC is enabled in Windows 7 a dialog displays the message An unidentified program wants to access your computer Click Allow Upgrading ImageQuant LAS 4000 Control Software Step Action 1 Uninstall the current version of ImageQuant LAS 4000 Control Software 2 Install the new version of ImageQuant LAS 4000 Control Software ImageQuant LAS 4000 User Manual 28 9607 42 AC 135 8 After sales service 8 1 8 2 After sales service Warranty 1 The warranty period will expire after 1 year from the date the system was delivered 2 GE Healthcare will make repairs free of charge for failures during the warranty period provided that normal usage conditions and the instructions given in this manual etc are followed 3 Repairs of the following failures will be charged for even if the warranty period has not yet expired e Problems caused by incorrect usage and or by any products other than those authorized by GE Healthcare and or problems caused by other equipment e Problems and or damage due to moving transport and or falling Repairs 1 Before asking for repairs refer to the troubleshooting section in Getting Started with ImageQuant LAS 4000 2 Ifthe problem persists fill in the service report fax sheet at the end of this manual and contact your GE Healthcare representative 3 The guaranteed repair service period of this product is 5 years from the termination of
64. ith the EtBr trans method 4 3 Calibration Ensure that the ImageQuant LAS 4000 is properly calibrated See Section 5 12 1 CCD Calibration on page 111 and Section 5 12 2 Flat Frame Calibration on page 112 36 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 4 Placing the sample 4 Operating the ImageQuant LAS 4000 4 4 Placing the sample This section describes how to choose an appropriate sample tray and how to place the sample for exposure 1 Select a sample tray suitable for the type of exposure to be performed Detection Chemiluminescence Bioluminescence Sample type Membrane Epi tray Titer plate NP tray Fluorescence Gel UV Trans illumination UV trans tray Gel Epi illumination Epi tray Membrane Epi tray Digitization ImageQuant LAS 4000 User Manual 28 9607 42 AC Membrane Gel Coomassie silver stain Epi tray White trans tray 37 4 Operating the ImageQuant LAS 4000 4 4 Placing the sample 2 Place a sample on the sample tray For Epi tray a Place the sample directly on the tray For UV or White Trans trays a Cut outa gel sheet slightly larger than the sample size b Place the gel sheet on the Trans tray Gel sheet Sample Gel sheet Note The gel sheet can be used repeatedly After use wash it with mild detergent rinse with water then dry well A gel sheet can be reused about 20 times 38 ImageQuant LAS 4000 User Manu
65. latFrame Mode Launch Application Print Adjust Increment Digitization Epi illumination Timer Invert pixel data Suto Range YES O no Description Pixel data inversion is performed Pixel data inversion is not performed Timer ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format FlatFrame Mode Launch Application Print Adjust Increment Digitization Epi illumination Timer Cooling Stop Timer Suto Range 1 Ar If the ImageQuant LAS 4000 is not used during the chosen time the cooling will be automatically shut off and the system will enter power saving mode 108 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 10 Edit menu Auto Range ImageQuant LAS 4000 Preference CCD Cooling Root Folder File Format Auto Range selection in Repetition Increment mode FlatFrame Mode Launch Application r Print Adjust None d Increment Digitization Epi illuminatiorn Timer Suto Range Semiautomated d Automated d An image whose gradations are adjusted automatically is displayed when exposure type is Increment or Repetition e None An image is displayed without adjusting its gradations e Semiautomated An image is displayed with its light and shade highlighted e Automated An image is displayed with its light and shade more highlighted The effect is the same as when exposure type is Pre
66. les other than the power cable that comes with the equipment 2 In EU countries The cable is given the following CEE Certification Number VDE 6522 1570 8035 AIG DEMKO 116773 ASC SEMKO 9449029 KEMA 93 6830 04 KCS LB NEMKO P94102620 CEBEC 8652 El FIMKO 179615 02 OVE 0204 515 00 Mercury containing products label CAUTION x Ultraviolet ray lamps in this product contain mercury Wwhich must be recycled or disposed of in accordance with local state or federal laws LED safety This product is categorized as a class 1 laser LED device IEC60825 1 A2 2001 8 ImageQuant LAS 4000 User Manual 28 9607 42 AC 1 Introduction 1 1 Important user information LED light sources Red Epi light Wavelength 630nm Class 1 Green Epi light Wavelength 520nm Class 1 Blue Epi light Wavelength 460nm Class 1 RGB module Wavelength 630 520 and 460nm Class 1 UV Epi light Wavelength 365nm Class 1M NIR Epi light Wavelength 710nm Class 1 White Epi light Wavelength 470 740nm Class 1 CAUTION Do not look at the light directly through optical instruments CAUTION If the door is opened and the interlock is cancelled class 1 laser LED will be emitted CAUTION Use of controls or adjustments or performance of procedures other than those specified in the user documentation may result in hazardous radiation exposure Notes and tips Note A Note is used to indicate information that is important for
67. lights in matching pairs only If Epi lights with different wavelengths are used the intended image will not be obtained ImageQuant LAS 4000 User Manual 28 9607 42 AC 55 4 Operating the ImageQuant LAS 4000 4 Operating the ImageQuant LAS 4000 This chapter describes the ImageQuant LAS 4000 Control Software and how to expose gels membranes or films to obtain digital images 41 Layout of the ImageQuant LAS 4000 Control Software The exposure of samples in the ImageQuant LAS 4000 the setting of exposure conditions and the viewing of the digitized images are performed within the ImageQuant LAS 4000 Control Software The following illustration shows the features of the main window of the ImageQuant LAS 4000 Control Software Description Description 1 Menu bar 6 The setting state such as the tempera ture condition of CCD is displayed 2 Exposure Type 7 The state of the Intelligent dark box IDX Sets the exposure method is displayed 3 Exposure Time 8 Method Tray position Sets the exposure time Sets the detection method and tray position 34 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 1 Layout of the ImageQuant LAS 4000 Control Software Description Description Sensitivity Resolution Focusing Sensitivity can be set Adjusts the focus and position Add Digitization Image Start A digitization image is exposed simulta Starts the exposure neously with a che
68. ling Root Folder Print Adjust Digitization Epi illumination Timer Invert pixel data Auto Range 5 4 Program settings Program Settings are used to set exposure time and exposure intervals when Program mode is selected as Exposure Type The input settings are saved as a template file 12 ll Program Exposure Setting 1 Template __ Newtempfte f YI Sensitivity Standard Y Template Comment Exposure Count 1 v 1 O Image 1 Image 2 Image 3 Image 4 Exposure Exposure Exposure Exposure Image 5 Image 6 Image 7 Image 8 Wait 0 Wait 0 Wait 0 wait Exposure 1 Exposure Exposure 1 Exposure Image 9 Image 10 Image 11 Image 12 Exposure 1 Exposure Exposure 1 Exposure Image 13 Image 14 Image 15 Image 16 wor e Lev wa we o Lev wee Exposure 1 Exposure Exposure 1 Exposure File name File Format Gel File Format gel v Save Folder C Documents and 5 ant LAS 4000 Image Save As Template 8 90 ImageQuant LAS 4000 User Manual 28 9607 42 AC Description A template file is selected If a new template is to be created select New Template Note When the selected template is modified Modified will be dis played 5 Software reference guide 5 4 Program settings Description Select the image file format to be saved Note Two types of format can be selected the GE Healthcare gel format gel and a linear 16 bit grayscale tiff file for mat tif Set
69. lluminator 312 nm ImageQuant LAS 4000 User Manual 28 9607 42 AC 45 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr Place a gel sheet which has been cut out to be slightly larger than the sample onto the UV trans tray Place the sample on the gel sheet Put the tray into the ImageQuant LAS 4000 and close the door Note The 605DF40 filter is normally used If this filter is currently not in the filter turret it should be installed Refer to Section 3 1 Changing or installing a filter on page 19 Note Ensure that the UV transilluminator is installed For instructions on changing the trans light source see Section 3 3 1 Changing the UV transilluminator or white light table on page 30 Sea LAS doe i it Y ipton ep Image Wer Eanccore Trog 46 ImageQuant LAS 4000 User Manual 28 9607 42 AC Step 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr Action Click the Method Tray position button Result The Method Tray position dialog opens Method Tray position Method Light Filter iris Chemdanrescerce Nore Though Foos Plssrescence esce res Oigtization Epbiuminstion White Cpedumination Though r2 0 are Bumination White Tren Buminanion Through P29 105x 70 mm 1 O2 O3 w Os OK Cancel e Select Fluorescence as the Method and choose EtBr from the pull down menu The 605DF40 filter is automatically selected e S
70. mageQuant LAS 4000 Control Software DVD and click the Browse button 11 Select to install the driver from the ImageQuant LAS 4000 Control Software DVD Lookin inage uank LAS 40000 lt O Ge gt My Recent Documents D E Cheek tog i My Document My Recent My Corrente a Local Dick C 8 ree 1 LAS 4000 Documnerds STORE N GO E Server on Hol SRST E Levees Daiko My Documents My Comptes Fie nane ii x Oper l Uy Netrcck Fier of yoe Cace i ImageQuant LAS 4000 User Manual 28 9607 42 AC 117 6 Installing ImageQuant LAS 4000 Control Software 6 2 Install ImageQuant LAS 4000 Control Software under Windows XP Step Action i 13 14 15 16 17 18 Open the USB Control folder Lock ine inage uart LAS 400010 Of s OUB furn My Racert Documeris Detkico My Documents My Cometes Fie name nl v Open j Hy Natmad Fier of yoe Cece f Select the DevMng inf file and click the Open button mk O U58 Conio Click the OK button in the Install from disk dialog Click the Next button in the Add hardware wizard Click the Next button again Click the Continue Anyway button in the Hardware Installation dialog Hardware Installation The software you are installing for this hardware FLA LAS USB Device Managment Driver has not passed Windows Logo testing to verify its compatibility nt with Windows xP Tell me why this testing is important Co
71. miluminescence im age Chemiluminescence method only Note The software needs to be started once after installing from an account with administrator priveleges This is to ensure that necessary folders and files are created 4 2 Preparation for exposure Analyzing PC Operation 1 Turn on the ImageQuant LAS 4000 computer and peripheral equipment ImageQuant LAS 4000 User Manual 28 9607 42 AC 35 4 Operating the ImageQuant LAS 4000 4 2 Preparation for exposure Step Operation Start up ImageQuant LAS 4000 Control Software EEEE ImageQuant LAS 4000 is in self diagnosis A message is displayed until the ImageQuant LAS Please watt 4000 Is ready ANSSI S Note The power switches of the instrument and PC can be turned on in any order Wait until the CCD has reached the preset cooling temperature and is ready for use ImageQuant LAS 4000 Fie EGR Vew Oplun Help ImageQuant LAS GO Expo sure Type Predsion Exposure Time Ato O Manual Sensitivity Resolution Add Digitization image ON Flat frame MKO L ma a z lj MetnodrTay position Lim Focusing m Tip e The instrument will be ready in a few minutes The power LED on the instrument is lit blue when the instrument is ready e Method Tray position and Focusing can be prepared even if the CCD has not cooled completely e The Start button can be clicked even when the CCD is not completely cooled w
72. n Epi light 520 nm Blue Epi light 460 nm RGB Module Epi light with red green and blue LEDs UV Epi light 365 nm White Epi light Sample tray Supports the sample Lifting table Moves the sample to the selected tray position Lens F0 85 43 mm LAS High Sens lens or F1 8 24 mm wide view lens ImageQuant LAS 4000 User Manual 28 9607 42 AC 2 The ImageQuant LAS 4000 2 2 Inside the ImageQuant LAS 4000 Description Lens connector Connects lens to IDX Automatic filter Used to hold filters selected in the control software changer The following filters are commonly used IR785 Alexa filter for NIR LED R670 Cy5 filter for red LED 575DF20 Cy3 filter for green LED 605DF40 EtBr filter for detecting EtBr 510DF10 filter for detecting GFP Y515 filter for blue LED L41 UV filter for UV LED Trans light source Connects the UV transilluminator or White light table connector to IDX Trans light source 312 nm UV transilluminator White light table Note Do not use connectors other than those supplied for the Epi light Sources Note Do not connect cables other than those supplied to the lens Note Do not connect anything other than the transmitted light sources to the transmitted light source connector 2 5 Connections Camera head 2 i 1 o o na ImageQuant LAS 4000 User Manual 28 9607 42 AC 13 2 The ImageQuant LAS 4000 2 3 Connections
73. n button Tip Click on the image to magnify it Click on the image again to return to the original size Select Exposure Type Exposure Type 5 N v Precision Increment Repetition Program 6 Set the Exposure Time or Interval Time 82 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 15 Exposing large sample using wide view lens Step Action Select Sensitivity Resolution Sensitivity Resolution High Resolution Standard High Super Ultra High Binning Super Binning Ultra Binning Tip Select Sensitivity Resolution in the Help menu You can display Help that describes the relation between sensitivity and resolution i r Click the Start button Exposure is started ImageQuant LAS 4000 User Manual 28 9607 42 AC 83 4 Operating the ImageQuant LAS 4000 4 15 Exposing large sample using wide view lens Action Adjust the gradations of the exposed image then save and print the image Click the Complete button ImageQuant The display returns to the main screen 416 Creating anew method and performing flat frame calibration For most purposes a pre existing method will be sufficient For other filter light source and iris combinations a new method needs to be created The following describes how to create a new method and perform a Flat Frame calibration Operation In Preference in the Edit menu select FlatFrame mode and set this to Ma
74. nen eee ees 77 Ats Ending the SessiON sirarni EN EE 80 4 15 Exposing large sample USING wide view lens ou eessesssesssecssesssessseesssssssssssecssecssecssecsseesseesseeeses 80 4 16 Creating a new method and performing flat frame calibration ss ss1s1ss01s0000000002 84 5 Software reference guide cscccssccssccsssssssccsccesscscsecssesseeesecesceessseseessseeseees 87 sA EXPOSUTE TYPO on cecsssscssccssssssssssssccsccssessusssessesecsssossonsnssuccecoscassnssucsucoscassassaesucssesecaesassassassscassessensanssesees 87 5 2 E E E r EN A EA EEEE 88 5 3 Ada amp digitization IMAgE tame nen enn ese oY On RNN 89 oY oo 2 1110 RS lt 1 6 Ln enon ee AA A eE ee EER 90 a5 FS UNG cece tage E teens A EE AEE E O E eave nade ecies 92 ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Table of Contents oio E 8 E ET mea AE E ne E A et ee ne E A N ome eee nee 93 5 7 Sensitivity Resolution saecteserscisevscoacascanssssstveauss es tsuaxagisnsbapstieeadsinevedssaciiessuesteverantueinsadadntudanteceaniens 97 56 Method Tay POSION speeismieeisirsetrrn e iiaei eiaeaen eten Ennai EAE aa aah aN aE EES ny a nee 98 T PE a E EE E E aici 101 TAO EE O E N AE A A AN 102 Sd AONTA e E ee Cae 109 s GPO A E E E E E ERN 111 SA CCA OT a A E E EAE E AE 111 De FPE TTE T a r A ninctany niensnnntenmmannasmenetaunmhins 112 e a es 2a N a A ee ESr TTP 113 S gt 8 2 6 er E A E E AAE EEE 114 6 Installing ImageQuant LAS 4000 Control Software
75. ng sequence 1 Install the USB contol driver 2 Install the USB function driver Windows XP only 3 Install the ImageQuant LAS 4000 Control Software 6 2 Install ImageQuant LAS 4000 Control Software under Windows xP Before you begin Log in using a Windows account with administrator privileges Install the USB Control Driver Windows XP Step Action 1 Disconnect ImageQuant LAS 4000 from the computer 2 Open the control panel and select Printers and Other Hardware 3 Click Add Hardware 4 Click the Next button in the Add hardware wizard 116 ImageQuant LAS 4000 User Manual 28 9607 42 AC 6 Installing ImageQuant LAS 4000 Control Software 6 2 Install ImageQuant LAS 4000 Control Software under Windows XP Step Action 5 Select Yes I have already connected the hardware and click the Next button 6 Select Add a new hardware device and click the Next button Ve tyler handara on ah rby mal aler o prea epee mm ha bint baise sici py rehale hd do enci ed io ce ipn ou mg de aig To wii heders ro bass n Pe ia ici Eii res hems a 7 Select Install the hardware that manually select from a list Advanced and click the Next button 8 Select Show All Devices and click the Next button Previn tom bot medion arira Phr Ippe od fmia prag ioe ret aliro ga ad E gudro eee Pre badene camegery po ard cho Le A Deen oe C ne Cee 9 Click the Have Disk button in the Add hardware wizard 10 Insert the I
76. nstal to tus folder o cick Change to instal to a different fokier Install ImageQuant LAS 4000 te C Program Fies e Click the Next button to install the software in the default folder C Program Files e Click the Change button to select a different location 6 Click the Install button Ready to Install the Program The wizard n ready to begri retaliation Chick instal to begin the instafaSon M you want to review or change any of your installation cettings cick Rack Cick Cancel to ent The wizard 7 lf User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified programs wants access to your computer Click Allow ImageQuant LAS 4000 User Manual 28 9607 42 AC 131 6 Installing ImageQuant LAS 4000 Control Software 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 8 Click Install this driver software anyway in the Windows Security dialog ee iF Windows Secunty Don t install this driver software You should check your manufacturer s website for updated driver software for your devece Only install driver software obtained from your manufacturer s website or dnc Unsigned software from other sources may harm your computert or steal indoemation vy See details 9 Click the Finish button to complete the installation InstaBShiek Wizard Completed The instaishreld wizard has successfidly retalled ImageQuant LAS 4000 Cick Fini
77. ntinuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now an contact the hardware vendor for software that has passed Windows Logo testing i STOP Installation Click the Finish button to complete the driver installation 118 ImageQuant LAS 4000 User Manual 28 9607 42 AC 6 Installing ImageQuant LAS 4000 Control Software 6 2 Install ImageQuant LAS 4000 Control Software under Windows XP Install the USB function driver Windows XP Step Action 1 Connect the computer and the ImageQuant LAS 4000 with a USB cable and turn ON the power switch of ImageQuant LAS 4000 The scanner is automat ically detected by the computer In the Found New Hardware Wizard dialog choose No not this time i oloome to the Found Mow Hardware Wizard Click the Next button in the Found New Hardware Wizard dialog Insert the installation DVD Select Install the software automatically Recommended Click the Next button in the Found New Hardware Wizard dialog Click the Finish button to complete the installation Install ImageQuant LAS 4000 Control Software Windows XP Step Action 1 2 Insert the ImageQuant LAS 4000 Control Software DVD Locate and double click the file ImageQuant LAS 4000 msi In the ImageQuant LAS 4000 InstallShield Wizard click the Ne
78. nual 84 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 16 Creating a new method and performing flat frame calibration Step Operation To create a new method click Method Setting in the Option menu The Method Setting dialog opens Method Setting Method List Name Light Filter Cy2 RGB EJ Blue Epi RGB ysispi Foes Cy2 E Blue Epi Blue ysispiFo es Cy3 RGB E Green Epi RGB 5750F20 Fo 85 2 cy3 E Green Epi creen 5750F20 F0 85 Cy5 RGB Bl Red Epi RG R670 F085 cy5 BB Red Epi Red R670 Faas DAPI BB vy Epi uv 4 F2 8 Digitization Epi illumination JJ white Epi white Through F2 8 Digitization Trans illumination J white Trans white Through F2 8 Select an existing method to change and click the Edit button or to create a new method click Add The Method Setting edit dialog opens Method Setting 3 Name TomsMethod Light Blue Epi Blue Fiter r720 Tris F0 85 Select the appropriate Light Filter and Iris edit the Name for the method and click 4 OK Once the method is added to the list of methods click OK to return to the main window ImageQuant LAS 4000 User Manual 28 9607 42 AC 85 4 Operating the ImageQuant LAS 4000 4 16 Creating a new method and performing flat frame calibration Operation Insert the calibration plate appropriate for the li
79. onnector 250 V AC 10 A Cable CENELEC OC 3 x 1 0 mm 70 C Power supply cord length Maximum 3 m ImageQuant LAS 4000 User Manual 28 9607 42 AC 145 A Appendix A 4 Main specifications Installation condi Placement conditions tions Free space required around ImageQuant LAS 4000 Secure space for maintenance work as follows Front 600 mm Right 600 mm Left 600 mm Rear 200 mm Top 1000 mm Table strength The allowable load must be 981 N m 100kg m or greater Other conditions 1 Decide on an installation location taking into consideration the work flow and ancillary facilities to be used 2 Required construction work and electricity air conditioning work must be completed in advance 3 Itisnot desirable to have a heat source on the IDX right side face where there is an air intake fan even if the environmental requirements have been met 4 Donot install the equipment near a window to avoid direct sunlight Attach a blind to nearby windows 5 Donot place objects near the power outlet so that you can disconnect the power cable from the outlet in case of emergency Floor oscillation conditions 1 Operating time Oscillation 0 03G 5 to 60 Hz Impact 1G 2 Non operating time Oscillation 0 4G 5 to 60 Hz Impact 2G 146 ImageQuant LAS 4000 User Manual 28 9607 42 AC A Appendix A 4 Main specifications Environmental conditions Operating temperature humidity conditions Tem
80. otherwise an incorrect image will be obtained e Donot insert any connector other than those for the Epi light Sources USB cable USB cable Connects ImageQuant LAS 4000 and the analysis PC Read data will be sent from a ImageQuant LAS 4000 to the analysis PC through the USB cable USB 2 0 is supported El Connect one end with the Im AE mee ageQuant LAS 4000 camera head USB connector and the other to a USB port of the analysis PC Note e Use the USB cable supplied with the equipment e Do not connect the instrument and the PC via a USB hub e Do not connect USB equipment other than ImageQuant LAS 4000 to the PC Similarly do not use other USB equipment con nected to the PC while ImageQuant LAS 4000 is in use This may result in the loss of image data e ThePC must be certified according to UL60950 1 UL listed and IEC60950 1 ImageQuant LAS 4000 User Manual 28 9607 42 AC 15 2 The ImageQuant LAS 4000 2 4 Parts and accessories 2 4 Parts and accessories Lj Epi tray F0 85 43mm LAS high sensitivity lens Cal plate GR Pink Cal plate DI Cal plate FL Green Lo White trans tray UV trans tray Gel sheet White light table UV transilluminator 16 ImageQuant LAS 4000 User Manual 28 9607 42 AC DO The ImageQuant LAS 4000 2 4 Parts and accessories F1 8 24mm wide view lens F mount adapter two RGB mo
81. perature 15 C to 28 C with temperature fluctuation below 10 C per hour or lower Humidity 30 to 70 RH no dew condensation When the above conditions cannot be satisfied modify the facilities accordingly Transportation storage conditions Temperature 25 C to 70 C Humidity 5 to 100 RH no dew condensation Installation location conditions 1 Do not install the equipment in an area where the temperature varies widely 2 Donot install the equipment near a source of heat 3 Donotinstall the equipment in an area where it may get wet or flooded 4 Do not install the equipment in an area where it may be exposed to corrosive gas 5 Do not install the equipment in a dusty area 6 Do not install the equipment in an area constantly or excessively ex posed to oscillations or impacts 7 Donot install the equipment in an area exposed to direct sunlight Operation site Indoors Maximum operating altitude 2000 m or lower Overvoltage category category II Transient overvoltage Rated pollution applied Pollution Degree 2 Analysis unit inter USB 2 0 face Do not connect the ImageQuant LAS 4000 USB connector to a computer not certified with UL60950 1 UL listed and IEC60950 1 ImageQuant LAS 4000 User Manual 28 9607 42 AC 147 A Appendix A 4 Main specifications Others Noise 70 dB A or lower A 5 Minimum computer requirements Operating system Microsoft Windows XP SP3 32 bit
82. rd if prompted then click Continue to proceed with the installation Action Insert the ImageQuant LAS 4000 Control Software DVD Locate and double click the file ImageQuant LAS 4000 msi In the ImageQuant LAS 4000 InstallShield Wizard dialog click the Next button Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative See the back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button ImageQuant LAS 4000 User Manual 28 9607 42 AC 123 6 Installing ImageQuant LAS 4000 Control Software 6 3 Install ImageQuant LAS 4000 Control Software under Windows Vista Step Action 5 10 Select destination folder in the dialog Bl ImageQuant LAS 4000 InstallStueld Weerd gem Destination bolder Cirk Hest bo inaia ts this fader er cick Change to inehall tn a dierent Pide z nstal Imagetsusnt LAS 4000 ko CH Pregram Files Change ee Gem e Click the Next button to install the software at the default folder C Pro gram Files e Click the Change button to install to a different folder Click the Install button If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow Click Install this driver software anyway Only install drever software obtained from your manufacturer s w
83. rect Epi lights and filter are installed See Chapter 3 Exchanging accessory parts on page 19 Place a sample on the Epi tray Put the tray into the ImageQuant LAS 4000 and close the door Note e Make sure that sample is suitably placed on the tray for the desired tray position e Ensure that a suitable method exists and that the ImageQuant LAS 4000 is properly calibrated See Section 4 16 Creating a new method and performing flat frame calibration on page 84 ImageQuant LAS 4000 User Manual 28 9607 42 AC 51 4 Operating the ImageQuant LAS 4000 4 7 Exposing fluorescence Epi illumination fie tdt Yew Opto Heb ImageQuant Do Exp n Ads 2 ut posure Type osure Time Ato gt Mna C v7 sitivety Resolution id Digitization image Clon t frame o Action Click the Method Tray position button Result The Method Tray position dialog opens Method Tray position Method ght Filter a C Chemdunrescerce Nore Through Foos 4 Plsorescence C a 7 erreze rass Cegneshon Epliumination White Lpedumination Teoh r2 0 Wand Bumination White Trar Bumination Trough P2 9 Tray position i 2 3 w J4 OK Cancel e Select Fluorescence as the Method choose the appropriate fluorescent marker and the appropriate light source from the pull down menus The appropriate filter is automatically selected e Select the Tray position e Click the OK button 52 ImageQuant LAS 4000 User Man
84. s you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Insert the ImageQuant LAS 4000 Control Software DVD 2 Locate and double click the file ImageQuant LAS 4000 msi 3 Inthe ImageQuant LAS 4000 InstallShield Wizard dialog click the Next button Welcome to the InsteiShieki Wizard for ImageQuant LAS 4000 The instaistreid R Wigard wil instal ImageQuant LAS 4000 on your computer To continue cick Next WARNING Ths program n protected by copyright lew and menawa vestes O 130 ImageQuant LAS 4000 User Manual 28 9607 42 AC 6 Installing ImageQuant LAS 4000 Control Software 6 4 Install ImageQuant LAS 4000 Control Software under Windows 7 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative See the back cover of this manual for contact information Peace read the foloveng kerse ageement carey GE Healthcare STANDARD SOFTWARE END USER LICENSE AGREEMENT FOR LIFE SCIENCES SOFTWARE PRODUCTS IMPOR TANT PLEASE READ CAREFULLY I do not accept the terms r the icense agreement insta reis Select I accept the terms in the license agreement and click the Next button 5 Select the destination folder in the dialog Ti imageQuant LAS 400 Destination Faker Cick Nex to i
85. sed to be uniformly emitted from the whole image Center CCD Calibration Data State of image that is not corrected Center 4 X lt Raw image CCD Calibration Data Electrical noise component Center FlatFrame Removal of noise component Center 4 DXRaw image CCD Calibration Data FlatFrame Optical unevenness Center Limb darkening due to optical characteristics Center A 2 Quick reference functions Function Function name Set item Exposing for a fixed expo Exposure Type Precision sure time Exposing consecutively Exposure Type Increment Exposing repeatedly Exposure Type Repetition Determining exposure Exposure Time Auto time automatically Entering and determining Exposure Time Manual exposure time 138 ImageQuant LAS 4000 User Manual 28 9607 42 AC A Appendix A 2 Quick reference functions Function Function name Set item Determining a desired Exposure Type Program exposure time and ex pose Setting the exposure Exposure Type Program settings time and the exposure intervals Sensitivity Resolution High Resolution Standard High Setting sensitivity and super resolution ltr High Binning Super Binning Ultra Binning Exposing a chemilumines Add Digitization Image On cence and a digitized white epi illuminated image by single click op eration Method Chemiluminescence Selec
86. sh to ent he wizard 132 ImageQuant LAS 4000 User Manual 28 9607 42 AC 7 Uninstalling and upgrading ImageQuant LAS 4000 Control Software 7 Uninstalling and upgrading ImageQuant LAS 4000 Control Software Before you begin Log in using a Windows account with administrator privileges Uninstalling ImageQuant LAS 4000 Control Software under Windows XP Step Action 1 Open the control panel and select Add or Remove Programs 2 Select ImageQuant LAS 4000 and click the Remove button Add or Remove Programs Gi Currently installed programs and updates Show updates Sort by Name Change or Remove Programs Click here for support information Used X ImageQuant LAS 4000 Size To change this program or remove it From your computer click Change or Remove Change Add New p Intel R Graphics Media Accelerator Driver Programs E Intel R Management Engine Interface Size 0 01MB ES Intel R Network Connections 13 1 33 0 Size 4 89MB Ad 4 amicus E Intel Active Management Technology Size 0 18MB Windows 5 Java TM 6 Update 7 Size 171 00MB Components E Microsoft NET Framework 1 1 amp j Microsoft NET Framework 1 1 Hotfix KB928366 Set Program p Microsoft NET Framework 2 0 Service Pack 2 Size 185 00MB secre j5 NET Framework PrexP Installed On 6 11 2009 jB ke958481 Installed On 6 11 2009 p Dr Watson Installed On 6 11 2009 j5 NET Framework CRT Installed On 6 11 2009 i NET Framework
87. solution Standard High Super Ultra High Binning Super Binning Ultra Binning Tip Select Sensitivity Resolution in the Help menu You can display Help that describes the relation between sensitivity and resolution 5 Sensitivity Kesolution Click the Start button The orange Busy LED lights on the instrument and a progress dialog is displayed during exposure ImageQuant LAS 4000 User Manual 28 9607 42 AC 65 4 Operating the ImageQuant LAS 4000 4 9 Exposing dye stained samples and films Trans illumination Step Action Adjust the gradations of the exposed image then save and print the image Click the Complete button The display returns to the main screen 410 Exposing consecutively Increment The Increment exposure type enables a series of images to be exposed consecutively The data for each image is added to the previous images to provide accumulated exposure data This section describes how to perform this type of experiment Place a sample on the appropriate tray Place the tray in the ImageQuant LAS 4000 then close the door Note e Make sure that the tray is placed at the required position 66 ImageQuant LAS 4000 User Manual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 10 Exposing consecutively Increment Action Click the Method Tray position button Result The Method Tray position dialog is opened Method Tray position Chemiluminescence None T
88. sssrssrersrrsrrsrrrrrrrrrrrrrrrrns 27 33 Cnonging the Igni SOUrCES seeman ERA 29 3 3 1 Changing the UV transilluminator or white light table usss 30 3o22 Chondnd Tep IONS sesesncreiirsunan iana 31 4 Operating the ImageQuant LAS 4000 eeeseeseessesserserserseeseeseesseeseeserseeseeseessees 34 4 1 Layout of the ImageQuant LAS 4000 Control Software us 34 4 2 Preparation for EKPOS TE sessinasncnensnain ana Eare E EA KRETE 55 E EE D E e NO deca T AE E E wees E E E AE E ove E E E TA E E 36 44 Placing the Sampla een meee Ot E 37 4 5 Exposing chemiluminescent samples oo eeececcssssssessseesseesssecssecssecssecesecssssssussssscssecssecsseesseeeseeeses 40 4 6 Exposing fluorescent samples EtBr ou ceeccsssssseesssessseccssecsssecsseecssscessecssecsssecsseccssecsssecsscessecs 45 4 7 Exposing fluorescence Epi illumination sscceccessccssscsssopsscaiscisaccasted catessesscesecbasbadetavecestcaanteseosvions 51 4 8 Exposing dye stained samples and films White Epi light s sssssssssssssssrrrrrerrrreess 56 49 Exposing dye stained samples and films Trans illumination sssssssrsrrssrseessssssssss 61 4 10 Exposing consecutively Increment o eececccsescssessseesssessscsssecsssesssecsssscsssecssecsssecesecsssecsssecsseeessecs 66 4 11 Exposing repeatedly Repetition ec cccsssssessseesssssssscsssecsssessseesssscssscsssecssuccsseesssecsssecsseesssecs 71 4 12 Printingexposed iMOgJES neseniai Ea E a a EE Eaa era 76 Stan SWN Epo ed IMIE eE ER
89. the filter changer is down proceed to step 4 2 Select Option Lens Exchange LAS 4000 elem Help CCD Calibration FlatFrame Calibration FlatFrame Calibration Pro Filter Customization m Lens Exchange Method Setting e 26 ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 3 Removing the wide view lens A message is displayed Lens Exchange Close the door and click OK ImageQuant LAS 4000 control software will shut down Do you want to continue Click OK 3 Lens Exchange Switch off ImageQuant LAS 4000 instrument and change the lens Turn off the instrument using the power switch 4 Remove the F mount adapter and lens while pushing the release button provided on the lens Remove the wide view lens together with the F mount adapter by rotating it Remove the lens hood from the lens Place the lens cap on CO N O WM Place the end cap on the lens and the cap on the F mount adapter 3 2 4 Installing the wide view lens 1 Close the instrument door If the power is turned off and the filter changer is down proceed to step 4 2 Select Option Lens Exchange LAS 4000 elem Help CCD Calibration FlatFrame Calibration FlatFrame Calibration Pro Filter Customization Lens Exchange Method Setting all ImageQuant LAS 4000 User Manual 28 9607 42 AC 27 3 Exchanging accessory par
90. ting detection method Fluorescence EtBr Fluorescence SYBR Green Fluorescence GFP Fluorescence Cy3 Fluorescence Cy5 Fluorescence Dy781 Fluorescence DAPI Digitization DIA Digitization EPI ImageQuant LAS 4000 User Manual 28 9607 42 AC 139 A Appendix A 2 Quick reference functions 140 Function Changing tray position Adjusting focus Function name Method Tray position Focusing Set item Select the tray position from 1 to 4 Fine adjust the light using Brightness and the focus using Adjust Starting exposure Start Inverting image data Saving a file in TIFF for mat Edit Preference Digitization Epi illumina tion tab Invert pixel data Edit Preference File Format tab Select a 16 bit Linear TIFF format Saving window image di rectly Edit Preference File Format tab Select an 8 bit Color TIFF format Changing the tempera Edit Preference Set the temperature ture setting of CCD CCD Cooling tab Changing the folder of Edit Preference Specify the folder where file saving Root Folder tab a file is saved Fine adjusting the print Edit Preference Enter size in percent size Print Adjust tab Displaying saturated da ta in red View Paint saturated data Red Select and check Displaying image in Neg ative Gray View Negative Gray Select and check Displaying image in Posi tive Gray Vi
91. tion Current Filters Select Filters 1 Throw Through 2 Throw Through 3 Throw Through 4 Throw Through 5 Throw Through Filter down Cancel 1 Ensure that the camera head and PC are connected Turn on the instrument and the PC and start the ImageQuant LAS 4000 Control Software 2 Close the instrument door 3 Select Filter Customization in the Option menu in the ImageQuant LAS 4000 Control Software main window 4 Click the Filter down button ImageQuant LAS 4000 User Manual 28 9607 42 AC 19 3 Exchanging accessory parts 3 1 Changing or installing a filter The filter changer is lowered 5 Open the instrument door 6 Turn the filter changer so the desired position is easily accessible If a filter is already in place at this position unscrew it carefully and put it back in its cover 7 Screw the new filter into place 8 Select the appropriate filter from the drop down list and click the OK button Filter Customization Current Filters 1 Through 2 Through 3 Through 4 Through 5 Through Note Change Filter and Filter position Select Filters Through Y Thi Through 605DF40 515 Di 510DF10 IR785 R670 575DF20 The filter changer returns to the original position when you click the Start Focusing or Method Tray position buttons 3 2 Installing or exchanging the lens Two lenses can be used with the ImageQuant LAS 4000
92. tively select a time unit and type in an exposure time Exposure Time Auto Manual 10 hr Sensitivity vV sec High F 15hr 2hr 60min 30min 10min Smin 2min Chemiluminesce imin sec 1 2sec 1 4sec Flat frame 1 8sec rey 1 15sec E 1 30sec 1 60sec i 1 100sec When exposure type is increment or repetition Choose a time unit from the drop down list and enter an interval time Interval Time Manual 10 U w Sensitivity Resi Standard Tip The interval time can be set from 10 seconds to 2 hours For a UV transmitting light source the interval time can be set from 10 seconds to 1 minute Note An image can be exposed for up to 30 hours in Precision mode However the image quality and quantitative performance are only guaranteed for a maximum of 2 hours 5 3 Add digitization image Add Digitization image ON A chemiluminescence and a white light epi illuminated image are exposed by single click operation when this function is set to ON After exposure both chemiluminescence and white epi illuminated images can be saved This function can be used only when the method is chemiluminescence ImageQuant LAS 4000 User Manual 28 9607 42 AC 89 5 Software reference guide 5 3 Add digitization image Tip Click on Digitization Epi illumination in Preference in the Edit menu and select YES for Invert Pixel data ImageQuant LAS 4000 Preference CCD Coo
93. trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your procedures ImageQuant LAS 4000 User Manual 28 9607 42 AC 9 1 Introduction 1 1 Important user information Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 10 ImageQuant LAS 4000 User Manual 28 9607 42 AC 2 The ImageQuant LAS 4000 2 The ImageQuant LAS 4000 This chapter describes the features and part names of the ImageQuant LAS 4000 hardware and the connectors that connect the various parts of the equipment 2 1 The ImageQuant LAS 4000 exterior Description Camera head CCD cooling and image data output Indicator panel Status lights Power LED Lights when the power is on Busy LED Lights when an exposure is in progress Error LED Lights when an error is detected Power switch Power ON O Power OFF Intelligent dark box IDX Dark box ImageQuant LAS 4000 User Manual 28 9607 42 AC 11 2 The ImageQuant LAS 4000 2 2 Inside the ImageQuant LAS 4000 l l 12 Inside the ImageQuant LAS 4000 Epi light connector Description Connects the epi light to the IDX Epi light source NIR Epi light 710 nm Red Epi light 630 nm Gree
94. ts 3 2 Installing or exchanging the lens 3 2 4 Installing the wide view lens A message is displayed Lens Exchange Close the door and click OK ImageQuant LAS 4000 control software will shut down Do you want to continue Click OK 3 Lens Exchange Switch off ImageQuant LAS 4000 instrument and change the lens Turn off the instrument using the power switch 4 Remove the cap attached to the F mount adapter by turning it to the right F mount adapter 5 Remove the end cap from the wide view lens 6 Align the White point mark of the F mount adapter with the lens mark and turn to lock them together Hold the lens so that the red point is towards you F mount adapter Wide view lens 28 ImageQuant LAS 4000 User Manual 28 9607 42 AC 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 4 Installing the wide view lens 7 Fix the lens in position be turning it to the right 8 Remove the lens cap 9 Placel the lens hood on the lens QO Lens hood 3 3 Changing the light sources Both trans transmitted and epi incident light sources are available for exposure with the ImageQuant LAS 4000 This section describes how to change both types of light source The following table summarizes the properties of the various light sources Peak wavelength UV transilluminator 312 nm Transmitted White light table ImageQuant LAS 4000 User Manual 28 9607 42 AC 29 3
95. ual 28 9607 42 AC 4 Operating the ImageQuant LAS 4000 4 7 Exposing fluorescence Epi illumination Step Action Click the Focusing button Result the focusing controls appear fe ia gt pon Heb ImageQuant LAS 6000 Exposure Type buremere interval Time Maree 1 e Y Sensitivity Resotution Redwi y Repeat Lirit ue 100 times 1 100 2 Fous 3 Brightness O Acu iD a om frre E Return Confirm the sample position and focus Click the Return button Tip Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type Exposure Type 3 Pi v Precision Exposure T Increment O Auto Repetition Manu Program ImageQuant LAS 4000 User Manual 28 9607 42 AC 53 4 Operating the ImageQuant LAS 4000 4 7 Exposing fluorescence Epi illumination 54 Action Select Auto or Manual for Exposure Time Auto Exposure Type Precision hd Exposure Time Auto Manual Note Automatic exposure time setting may not be possible de pending on the sample type and method Manual Select an exposure time from the drop down list or enter the exposure time manually The exposure time can be set from 0 01 seconds up to 30 hours Exposure Type Exposure Time Auto Manual 10 hr Sensitivity sec Ste eel 1Shr Add Digitiz di Oon 60min 30min 10min Smin 2min Chemiluminesce
96. xt button Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button ImageQuant LAS 4000 User Manual 28 9607 42 AC 119 6 Installing ImageQuant LAS 4000 Control Software 6 2 Install ImageQuant LAS 4000 Control Software under Windows XP Step Action 5 Select destination folder in the dialog ie ImageQuant LAS 4000 InstallShield Wizard Destination Folder Click Next to install to this folder or click Change to install to a different folder ca Install ImageQuant LAS 4000 to C Program Files e Click the Next button to install the software at the default folder C Pro gram Files e Click the Change button to install to a different folder Click the Install button in the installation dialog Click the Finish button Start the ImageQuant LAS 4000 Control Software This will ensure that nec essary folders and files are created 6 3 Install ImageQuant LAS 4000 Control Software under Windows Vista Before you begin Log in using a Windows account with administrator privileges 120 ImageQuant LAS 4000 User Manual 28 9607 42 AC 6 Installing ImageQuant LAS 4000 Control Software 6 3 Install ImageQuant LAS 4000 Control Software under Windows Vista Install the USB Control Driver Windows Vista Note Step
97. y Position Note Administrator priveleges are required to perform Flat Frame calibration Flat Frame Calibration 1 Select Flat Frame Calibration in the Option menu The window below is then displayed FlatFrame Calibration Lite Digitization Epi illumination w Applicable Tray EPI Tray Uniformity Standard Calibration Plate DI Calibrate All Tray Position Tray Position 1 Rethy Sun May 31 18 15 22 2009 Read go pi Tray Position 2 Mon May 25 14 16 55 2009 Ready Tray Position 3 CL Mon May 25 14 17 36 2009 Ready Tray Position 4 Fi Mon May 25 14 18 16 2009 Total Time for Calibration 0 minutes Select a method from the drop down menu at the top Put the displayed tray and Calibration Plate in the instrument Select the condition to be created mn fF WN N Click the Start button Note Make sure that an appropriate filter and tray are in place 6 A message window opens when the calibration is completed Click OK 7 Click the Close button to close the FlatFrame Calibration window 112 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 12 Option menu 5 12 2 Flat Frame Calibration Flat Frame Calibration Pro 1 f FlatFrame Calibration Pro is selected in the Option menu the window of the conditions preset in the Method Tray position window will be displayed FlatFrame Calibration Pro Light Filter Tray Position White Epi White Through i 2 Lens FUJINON VRF43LMD
98. y drag The changed gradation is applied to all ging the mouse images Gradation can be changed by dragging Exposure is discontinued the mouse The current image is discarded Gradation conversion curve can be Click this tab to enter index view changed linear or sigmoidal Returns the gradation to the initial set Click this tab to magnify the selected ting image Tip e Exposure is interrupted when you click the Stop button during Precision exposure In this case the image in course of exposure is not saved e When you click the Apply All button during magnified image display the gradation changed in the expanded image display state Is applied to all magnified images Note Only the 100 latest exposed images can be saved for exposure types Increment and Repetition 94 ImageQuant LAS 4000 User Manual 28 9607 42 AC 5 Software reference guide 5 6 Start During exposure type Program Description The range can be changed by dragging Exposure is discontinued the mouse The current image is discarded Gradation can be changed by dragging Click this tab to enter index view the mouse The gradation conversion curve can be Click this tab to magnify the latest im changed linear or sigmoidal age Return to initial setting Preset conditions The changed gradation is applied to all The area where an image will be dis images played Tip e Images for which exposure is completed

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