Before initial use of this instrument, please carefully read this manual!
Contents
1. HE K RK amp RRR G eee 3 SS ou cue EEEE L ke EAR sg s RE FGERRR fost gt 2 Wawa EE E E Gl Ee s dela 5 alg A 5 A 5 E 8 EE gg BE 5 4 E 2 gg am 5 SX E UV EEUU ee HE ge EET 2 a ES 3 B Ei E LifePro Thermal Cycler Attachment 2 Attachment 2 Value and Meaning of Parameter Setting No Parameter Meaning Scope of setting Note value Temperature 1 Temp C control point 4 0 99 0 Celsius Temperature control time Temperature press plus minus to is infinitely long close 2 Time control ime set before hot lid at same time SE 00 00 59 59 Rate of 0 1 4 0 3 Ramp C S Temperature Maximum rate of risen ne temperature rise fall Celsius second P Temperature 4 Temp increment in each 0 0 9 9 cycle Celsius 5 Time Time increment in 0 00 9 59 each cycle m ss Cycle1 Paragraph number 1 5 x00 Cycle times in this 1 99 paragraph 6 First segment in X any segment Cycle1 x00 From 00 to From 00 this paragraph number already 00 existed to 00 Last segment in X415 this paragraph Gradient 7 Grad temperature 0 30 Celsius 8 Date Date YY MM DD oe ae Ten Year month day 9 Time Time HH MM SS 00 00 00 23 59 59 Hour minute second 10 Default File Default file name 11 characters in in system maxi2. LifePro Thermal Cycler Instruction for operation lt Press shift between running interface 1 and running interface 2 1 2 How to set system parameters Press System in main interface to access the interface for system parameter setting Press confirmation key to move the cursor to the next item Roll and select the items with N by cursor up down key or press character key to input information for other items press cursor left right key to move the cursor lt Press Back to return to main menu lt Press Save to save parameters Special caution press Save to confirm after system parameter setting or the system parameter won t change 1 2 1 Date Time indicate the default system time after system start 1 2 2 Default File Default User indicates the default running program name and user after system start 1 2 3 Control Mode indicates the default temperature control mode after System start including Block and Tube choices Hotlid off File running interface 2 File Name PCR1 User BIOER 1c Temp 00 0 Total Time 00h 56m 2009 05 1 10 05 00 Now Running Time 00 00 Cycle1 01 of 10 Remainig Time 00h 56m 95 0 950 950 950 950 950 950 950 95 0 95 0 950 as ee ones arr bez Interface for system parameter setting Hot
3. LIffalDrmra Thermal Cycler Bioer Serves Life oco 3 Instruction for buttons 6 Character key to input characters press button ABC DEF Gh JKL MNO continuously to input letters 0990090 PORS UV WXYZ 30008 Display mode switch key switch display mode between curve and text during running Plus minus key switch the plus minus for program temperature editing and time refining Cursor left right key press once to move the cursor left right Cursor up down key press once to move the cursor up down Confirmation key press button to accept the setting value on screen at file setting Menu key press button to accept the item above the button on screen LifePro Thermal Cycler Preparations 4 Check before start Check the following before insert the plug and power on the instrument 1 Whether the voltage of power supply matches the voltage required by instrument see Chapter II for requirements of power supply 2 Make sure the power line pug has inserted in power socket reliably 3 Earth the power line reliably 5 Start Power on turn power to the amplifier gives a du du sound and the fan starts to run which indicates the power supply is available Self testingeeeeee is shown on screen and then the instrument takes about 1 to 2 minutes for self testing Please wait with pat
4. Caution Turn the knob at anti clockwise direction as per diagram before covering the lid clockwise rotation for pressing anti clockwise rotation for loosing until a dang dang sound is heard Cover the lid and turn the knob at clockwise direction until a dang dang sound is heard and then turn another step When a dang dang sound is heard when knob is rotated at first regardless of clockwise or anti clockwise press the unlocking device by finger turn the knob at the opposite direction for two steps and then rotation knob on unlocking device can be released 1 How to run PCR program Power on the thermal cycler gives a du du sound which indicates the power supply is available Self testing is shown on screen and the instrument takes about 1 to 2 minutes for self testing Please wait with patience The main interface appears if no problem is found Main interface Shown on the upper left of main interface is the status of hot lid setting Hotlid off 2009 05 1 10 05 00 Shown in the middle of main interface is the information such as the Bioer information software version module type default BIOER program default user control mode sample amount and so on wae EC re Press File to access the interface for Block B 96G file list Default User BIOER Press System to access the interface Default File PCR1 for system parameter setting Control Mode Tube lt Pre
5. in main interface to access the interface for USB connection If USB flash memory is inserted as the figure at lefi shows Press Back to interface Press Input to select file input from USB flash memory it shows USB user list Hotlid off The Instrument has Connected UsbDisk Please Choose Input or Output File return to main Press F1 to Back lt gt Press Output to select to output file USB flash memory it shows local file user list Press F4 to Input File Press F5 to Output File Back Output LifePro Thermal Cycler Instruction for operation If no USB flash memory is inserted as the figure at left shows press Back to return to main interface 2 2 Input file from USB flash memory Press Back to return to interface of USB flash memory connection Press cursor up down to select copy file user lt gt lt Turn page by cursor left right if user number is more than 30 Press View to see USB file list Press Back to return to interface of USB flash memory user list Press cursor up down to select copy file Turn page by cursor left right if user number is more than 30 Press Copy to input file If copy is correct it shows the dialog box below File Saved If not it shows File input error o gt 9 9 2 3 Output file to USB flash memory Press Back to return to interf
6. Select file to change password Select Change File Password menu under File menu to bounce dialog box Input old password and new password click OK Prompt File name can t be changed if it is open User s Manual of LifePro Thermal Cycler Connection to computer Change File Password Do you want to change password of Computer gt TTTT gt 1 Enter old password Enter new password The password can contain only numbers Enter new password again Use empty password Jf OK x Cancel After file is edited click Save File item under File menu to save file mport file e Select user item on local computer e Select Export File menu under File menu Select file to import and click Open Prompt File can t be imported if file is already existed and open Export file Select file to be exported e Select Export File menu under File menu Select path for export and click OK Prompt File can t be exported if it is open 3 5 Tool menu How to Connect device Disconnect Calculate gradient Change device IP and device name Select device not connected click Connect menu Prompt User s Manual of LifePro Thermal Cycler Connection to computer One device can only connect to one computer Select device not connected click Disconnect menu Calculate gradient e Select gradient menu Input gradient initial point and gradient value Select 8 12 96 holes as calculation module t
7. and etc virus HTLV HIV HBV HCV HPVS EV CMV EBV HSV measles virus rotavirus parvovirus B19 and etc parasite malaria and etc human genetic disease Lesh Nyhan syndrome Mediterranean anemia hemophilia BMD DMD cystic fibrosis and etc Immunology HLA typing T cell receptor or antibody diversity qualification autoimmune disease gene mapping lymphokine quantification and etc Human genome project DNA mark generated from scatter repetitive sequence genetic map construction detect DNA polymorphism sperm drawing physical map construction sequencing expression map and etc Forensic medicine crime scene specimen analysis HLA DQ analysis and etc Oncology pancreas cancer rectal cancer lung cancer thyroid cancer melanin cancer hematological malignant tumor and etc Histology population biology genetic cluster research evolution research animal protection research ecology environmental science experimental genetics and etc Paleontology archeology and museum specimen analysis and etc Botany zoology animal inflectional disease diagnosis plant pathogen detection and etc 3 Features of LifePro Thermal Cycler LifePro Thermal Cycler is a PCR instrument with multiple functions and several applications Below are its features Use advanced thermoelectric refrigeration technology from ITI of US and thermoelectric refrigeration elements made under quality management of Japan which are steady
8. the plug shall be completely and tightly inserted into the socket and at withdrawal heavily pulling the wire shall not be allowed f Placement of instrument This instrument shall be fixed in a low RH and low dust place apart from water source e g water pool and water pipeline and the room shall be well ventilated and free of corrosive gas or and interference of strong magnetic field The instrument shall not be placed in a wet or dusty location The openings on this instrument are for ventilation circulation and in order to avoid over heat of the instrument body they shall not be blocked or covered When a single set or several sets of instrument LifePro Thermal Cycler Important Notes are used the interval between its surrounding ventilation opening and its nearest object shall not be below 50cm or 100cm Also don t use the instrument on loose or soft surface or the air inlet at instrument bottom might be blocked Excessive temperature would impair the test performance or result in fault of instrument This instrument shall not be used in location subjected to direct sunshine The instrument shall be kept away from hot gas stove and all other heat source If the instrument is to be set aside for a long time the power plug shall be withdrawn and the instrument covered with soft cloth or plastic film to avoid entrance of dust g Notes during operation During test cares shall be taken to prevent liquid from dropping onto the ins
9. 1 C 30 C Save the file Revise the file adjust the rate for temperature rise fall refine temperature and time for cycling automatically and display and calibrate the time Display the temperature time cycle times setting and real time value at file running including sound prompt total rest time estimation for file running failure protection and alarming Stop the file running including pause the file running stop the file running recover automatically after power off Caution The above mentioned software function is merely for reference The software function would be modified without notice LifePro Thermal Cycler Preparations Chapter Ill Preparations This chapter introduces the structure of instrument keyboard for operation function of buttons and preparations before start Please get familiar with the contents in this chapter for the first time to use this instrument 1 Structure diagram Note Hot lid 2 Keyboard 3 LCD screen USB interface 5 Ventilation hole 6 Block CORJA5 interface 8 Power switch 9 Power socket with fuse Caution The fuse specification must be 250V 8A 5x20 Use qualified product to replace the fuse Contact fuse supplier or manufacturer when necessary LifePro Thermal Cycler Preparations 2 Keyboard diagram BIOER VEE ABC DEF AX 8 GHI JKL MNO DeX PQRS TUY WXYZ
10. Accessible IPs MAC Address v Modify 00 90 8 10 E2 B7 7 7 hReal COM Mode Serial Number 3838 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Settings Click the Modify check box to modify configuration w OK X Cancel Select TCP Server Mode under Operating Mode Operating Mode 1 Port s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode TCP Server Mode TCP Server TCP Client Mode UDP Mode TCP Server Mode Settings Local TCP Port 4001 Max Connection 0 65535 ms Data Packing Optional Delimiter 1 0 ff Hex Delimiter 2 0 ff Hex Force Tx Timeout 0 65535 ms w OK X Cancel Click OK close Network Enabler Administrator 11 Restart the instrument access interface offer instrument system parameter setting and set system parameters again Caution Program version might upgrade without notice User can visit www bioer com cn to check and download the latest program version The device uses port 4001 as port number please assure the port is not shielded LifePro Thermal Cycler Failure analysis and handling Chapter VII Failure analysis and handling This chapter introduces the failures the instrument might have and the way to analyze and handle 1 Failure analysis and handling No Failure Analysis Handling Power is not connected Check BOWEN ANg
11. Configuration Changed NE 41105 01 Configuration Changed NE 41105 01 Configuration Changed NE 41105 01 Configuration Changed NE 4110S 00 Ny Network Enabler Administrator COM Mapping File Function COM Mapping View Help IDOA on Function COM Mapping 1 com Zo Network Enabler Admit Configuration COM4 Hi Performance FIFO Ena COM Mapping IP Address Report Configuration Changed NE 41105 00 90 E8 10 E2 87 Configuration Changed NE 4110S 00 90 E8 10 E 2 87 LifePro Thermal Cycler Firmware upgrading Double click netwerk number setting mapping property page of network number searched in list column and select Advanced Settings setting as the followingshewn belew Caution CON Port Settings Port Number 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Tx Mode Classical FIFO Enable _ Fast Flush Only Flush Local Buffer w OK X Cancel Select Basic Settings setting as the following LifePro Thermal Cycler Firmware upgrading COM Port Settings Port Number 1 Part s Selected 1st port is Port 1 Advanced Settings Serial Parameters COM Number COM3 curent in use if OK M Cancel Select Serial Parameters setting as the followingshewa below COE Port Settings Port Number 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Par
12. LifePro Thermal Cycler Connection to computer 3 3 User menu How to Create new file Change user name Copy user Paste user Delete user Import user Export user Create a user Select device name sub item in device list or any user name sub item in device name sub item Select create user menu under User menu it bounces a user dialog box Input user name and click OK Create User Do you want to create new user in Computer Enter new user name The user name can contain only letters and numbers vw OK x Cancel Change user name e Select Change user name Select Change User Name menu under User menu it bounces the dialog box to change user name Input user name and click OK Prompt User name can t be changed if the file under user name is open Change User Name Do you want to change user name of Computer s 123 Enter new user name The user name can contain only letters and numbers Jf OK x Cancel User s Manual of LifePro Thermal Cycler Connection to computer opy user Select user name to be copied by device list e Select Copy User menu under User menu aste user e Select User sub item in device list or device item in device list Select Paste User menu under User menu Prompt User can t be pasted if copy user is already existed and file is open Paste is only done between local computer and device elete user 1 Select
13. and user may affirm the above N Network Enabler Administrator Configuration File Function Configuration View Help z gi E mim rad Loc met iration 0 Network Enabler Module s No Model MACAddress IPAddess Status 0 1 To select search button 2 by using mouse then appear the following interface LifePro Thermal Cycler Firmware upgrading N Network Enabler Administrator Configuration Eie Function co View Help 222 2 2 gu Function I Configuration 0 Network Enabler Module s Network Enabler Admit MAC Address IP Address e Configuration 4 COM Mapping E Monitor Port Monitor XQ IP Address Report Searching Searching for Network Enabler Stop Found 1 Network Enabler s remain timeout 4 sec MAC Address IP Address NE 41105 00 90 E8 1C AF 60 192 168 0 250 Message Log 0 Monitor Log 0 us mme a o Now 2010 5 20 15 16 06 Z After connecting device normally the following interface will appear Nv Network Enabler Administrator Configuration File Function Configuration View Help Configuration E 1 Network Enabler Module s No Model MAC Address IP Address _ EE NE 41105 00 90 E81C AF 60 192 168 0 2 J Network Enabler Admit Configuration di COM Mapping Monitor Port Monitor X IP Address Report To double click the entry of the following graphic by using mouse or pitch on the ent
14. connect The screen doesn t turn on and a Fuse is burnt He 250V 1 du du sound is not heard after power on Switch is damaged Replace switch Others Contact supplier or manufacturer A du du sound is heard after power on The plug that connects Contact li 2 Please insert Block is shown on module and host machine ONC Supplier or screen a du alarm is heard is loose manufacturer Please check error is shown on Module sensor is screen while file running and one of the damaged or in poor following is prompted contact Contact s ppli 3 Temperature sensor disconnect The extwrnal environment PME Temperature sensor1 disconnect is too low will cause manufacturer Temperature sensor2 disconnect radiator alarm not Temperature sensor3 disconnect instrument anomaly Please check error is shown on Heat is d d Contact li 4 screen while file running and Heat api p s OE ppt OE sink sensor disconnect is prompted OKIN peor contact manuragiurer 5 E Acl A GIG Hot lid sensor is damaged Contact supplier or sensor disconnect is prompted or in poor contact manufacturer Remove things that Ventilation hole is blocked block ventilation hole Rate of module temperature rise eie sn GENER OBVIOUS OF demperatire Connection wire is loose wire fasten the bolt control is not accurate i nd on terminal if it is loose Refrigeration plate is Contact supplier or damaged manufacturer Ambient
15. devices online can t use a same IP address 2 Configure the device again if this instrument is used for the first time 3 Set same subnet for several devices online 4 The device doesn t support the function of acquiring IP address automatically 5 Use switch to connect for several devices online use cross network cable to connect if HUB is used User s Manual of LifePro Thermal Cycler Connection to computer 3 Main interface GenePro Filter all x eq106 Equipment state display z eq104 96G s a B User 54 System 1 egt Main display area 54 System Gy History li Setting Equipment list 3 1 Tool bar Amend user name Input file Copy file Change file code Grads calculate Output user Paste user New file Delet file Link equipment L ENE a T 255 RL Del ee o Copy user Copy user Sayo e Equipment setup Input user Paste file Setup user Delet user Edit file p Change file name Disconnect link 3 2 Basic operation e Open device or this computer s file Click run e Select type and volume for test tube Click OK to run Prompt Select device before running if file is saved in computer User s Manual of LifePro Thermal Cycler iew running file Connection to computer Run File A B is shown below the device name when device is running file Double click Run File A B to see running file contents and device running status Prompt Click pause
16. is saved normally the system shows File have been saved Please continue another operation Press confirmation key to make it disappear If file has the Caps same name with another the system asks to name again Rename or continue to save Save Press number key to set and save password Press Run to access the interface for file running if Control Mode is Block now press Run to bounce the dialog box below if Control Mode is Tube now Select Sample Volume 050ul It is the same with main interface confirm the password input the sample type and micro liter amount of sample size gt Caution 1 File name and user name are not case sensitive 2 Please remember the password 3 The password should be 0 9 numeric characters File running interface 1 As the file runs normally eee at Now Hot lid off 2009 05 1 10 05 00 Running keeps blinking The system User File Bioer Bioer shows File run over when running is completed Press Stop it shows Confirm stop running Temp 95 0C lt Select stop stop or continue Start as per information Time 01 00 lt Press Pause it shows Now pause running you can select continue Remain Time 00h56m Start as per information Press View File to view the edited PCR program lt gt Press Skip to skip the current temperature section and go to the next temperature
17. must be reliably earthed 2 Transportation and storage conditions Ambient temperature 20 C 55 C Relative humidity 38096 3 Basic parameters Specification TC 96 G H b A Sample size 96x0 2ml 96 micro plate 12x8 test tube bar 8x12 test tube bar Scope of temperature 4 C 99 C Heating Rate max 24 0 C Cooling Rate max 24 0 C Temperature uniformity 0 2 C 55 C Temperature control precision 0 1 C Temperature fluctuation 0 2 C Maximum gradient 30 C Minimum gradient 1 C Scope of hot lid temperature setting 30 C 110 C Scope of hot lid working temperature 105 C 5 C File saving amount 299 pieces Energy consumption maximum input power 600W Operation mode Continuous operation mode Outline dimension 351mmx279mmx250mm LxWxH Fuse 250V 8A d5x20 PC interface RJ45 Weight kg 10 LifePro Thermal Cycler Features Caution If the hot lid temperature setting is lower than module temperature setting the hot lid temperature shown on screen is higher than hot lid setting Software functions Edit the file including file number temperature and time of each segment cycle times setting file view revision and deletion automatic file connection gradient temperature setting the maximum paragraph step and cycle times for editable file are 5 16 and 99 respectively The scope for gradient temperature setting is
18. status Double click System to view instrument device storage status T GenePro User File Tools Help o 6O N h u h h h g 28233 amp 9 dBRL ETTER jj Computer eq104 gt system state a Fiter a a ul B ss Total memory size 51200 GRE Bi okfile S Used memory size 3072 eq104 96G S gt Free memory size 48128 Free memory scan store about 94 24 System amp eq104 2 8 User ag huang zx 984 Bi 51 x5 Set system parameters Double click Settings sub item under System items to set system parameters T GenePro EEK User File Tools Help o 089 o 9 pe a 4 E 4 5 Tow ES D Ce OM cy Ct a ex i h LX f al g 8 A amp O E il Computer eq104 gt system setting ES riter an me gul fault B ss BEIM eq106 B okfile Default hotlid temperature off 51 c s Beep alarm when run finish OFF eq104 96G S gt Key beep OFF Temperature control mode Tybe 24 System eq104 3 B User Default file ag huang Default user Module A Default hotlid temperature OFF amp Beep alarm when run finish OFF 34 System Key beep OFF OE Temperature control mode Block B RunFile Default user Default file Module B Default hotlid temperature Beep alarm when run finish Key beep Temperature control mode Default user Default file EM pens Elle pe oe d La User s Manual of
19. stop to pause stop equip GenePro User File Tools Help ment 2 22088 200 8aRR a FF aRel o s Bl Computer Ead ul B ss B okfile Bf a u2 eql06 User 54 System c eqi04 User a huang eq104 gt Run File Sement Tenp Tine AutoExtiontenp 1 20 0 00 20 4 0 00 50 4 0 00 20 4 0 00 40 4 0 Begin Segnent End Segment Cuvette volume 60ul Cuvette type 0 5ml Module temp 20 7C ES 53 40 0C0 LL 20 secs 50 secs State Running Total time 04h 21m Remaining time 04h 21m Hotlid temp 20 5C 54 55 00 40 secs Filter fei gt eq104 96G eq106 b gt GenePro User File Tools Help 22 amp 8 3a302 amp iu d Gm amp o lt Bl Computer ce ul B ss B okfile Bf ad u2 egl06 User m System f eq 104 User a8 huang a 984 B 51 a 5 a test 54 System Erion amp Setting B Run File eq104 gt file run log StartTime EndTime 2007 11 30 15 57 22 2007 11 29 10 15 53 2007 11 29 10 14 17 2007 11 29 10 13 39 2007 11 29 10 12 52 Run 2007 11 29 10 16 31 Complete 2007 11 29 10 15 37 Complete 2007 11 29 10 14 08 Complete 2007 11 29 10 13 27 Complete Filter all z eq106 eqi04 96G User s Manual of LifePro Thermal Cycler Connection to computer iew instrument device storage
20. temperature or Please adjust humidity is too high and ambient exceeds the operation temperature and Rate of module temperare fall slows condition of instrument humidity properly 7 1 down obviously or it cant fall below f room temperature Refrigeration plate is l damaged Contact supplier or Fan is damaged or doesn t manufacturer run Temperature sensor is 8 Module can t heat or cool damaged Contact supplier or All refrigeration plates are manufacturer damaged LifePro Thermal Cycler Failure analysis and handling n ore status onto Set hot lid status as OFF in system parameter setting a certain temperature interface Hot lid closes automatically since Set temperature temperature control time is control time in 9 Hot lid can t heat set as in file editing digital form interface Hot plug in is loose Heating element in hot lid Contact supplier or is damaged manufacturer Temperature sensor in hot lid is damaged Chip is in poor contact 10 Unusual character is shown on screen Er a or Chip is damaged manufacturer or Contact supplier or 11 Button on panel can t work Micro switch is damaged manufactir r Set hot lid temperature as per Hot lid temperature is not How to Set System set Hot lid is set as OFF Parameters in 1 2 of Chapter IV in this manual Adjust hole position 18 Se agenm eo evaporate
21. user to be deleted by device list 2 Select delete User menu under User menu Prompt User can t be deleted if file under user is open mport user e Select local computer Select Import User menu under User menu Select the directory for import click Open Prompt User can t be imported if user to be imported is already existed and user file is open xport user e Select user to be exported by local computer Select Export User menu under User menu Select the directory for export click OK Prompt User can t be exported if user file to be exported is open 3 4 File menu How to Create new file Open and edit file Copy file Paste file Delete file Change file name Change file password Save file Import file Export file reate new file Select user to create file User s Manual of LifePro Thermal Cycler Connection to computer Select Create New File menu under File menu to bounce dialog box 3 Input file name and password click OK Create File Do you want to create new file in 1 of Computer Enter new file name The file name can contain only letters and numbers Enter password The password can contain only numbers Enter password again Use empty password Jf OK x Cancel Edit file Double click user sub item in device list open file list double click edit file or create new file access the interface to edit file e Edit file conten
22. Caution The Bioer Co reserves the right to modify this manual at any time without notice The manual contains copyright protected and patented material Without prior written consent from Hangzhou Bioer Technology Co Ltd any part of the manual shall not be duplicated reproduced or translated into any other language Before initial use of this instrument please carefully read this manual File No BYQ60630000000ESM File version Dec 30JulyAprilFebruaryFebruarySepMay 2009 20123110 Version 1 2478653 LifePro Thermal Cycler Important Notes Important Notes 1 Usual practice Caution Important information is contained in any item and should be carefully read Failure to observe the instruction would result in damage or abnormal function of instrument Warning The warning message requires extremely careful operation of a certain step Failure to observe the instruction would result in serious personal injury 2 Safety During operation maintenance and repair of this instrument the following basic safety notes shall be observed In case of failure to follow these measures or the warnings or notes indicated herein the basic protection provided by the instrument its safety criteria of design and manufacture and its predicted use range would be impaired Hangzhou Bioer Technology Co Ltd shall be irresponsible for the consequence resulted from user s failure to observe the following requirements Note
23. Pro Thermal Cycler Firmware upgrading Configuration Information AutoWaming IPAddress Repot Password Digitall0 Serial CMD Model i Network i NE 41105 Basic Serial v Modify IP Address 192 168 0 245 Operating Mode Accessible IPs MAC Address 00 S0E8 10 287 input IP address Serial Number 3898 Netmask 255 255 255 0 Gateway input subnet mark Firmware Ver Ver 4 0 IP Configuration Static DNS Server 1 BIOS Ver Ver 2 1 DNS Server 2 Status Modify Data Mode public Click the Modify check box to modify configuration m4 OK x Cancel Causion Ip address and subnet mark are set according to network requirement of computer Open the property page select the Operating Mode item then click the settings button Configuration 3 cq Auto Warning IP Address Report Password Digital IO Serial CMD NE i i perating Mode i NE 41105 Basic Network Serial perating Accessible IPs MAC Address v Modify 00 90 E8 10 E2 B7 Port Alas UH ea COM Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Settings Click the Modify check box to modify configuration wv OK x Cancel Select TCP Server Mode under Operating Mode LifePro Thermal Cycler Firmware upgrading Operating Mode 1 Part s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode TCP Server Mode TCP Sev
24. This instrument conforms to Class I and Protection Class IP20 of GB4793 1 Standard and it is for indoor use a Instrument earth In order to avoid electrical shock accident the input power wire of instrument shall be reliably earthed This instrument uses 3 core earth plug and this plug is provided with a third earth leg is merely used for earth type power socket and is a safety unit If the plug is impossible to insert into the socket the socket shall be correctly fixed by electrician otherwise the earth plug would lose safe protection function b Keeping apart from the live circuit The operator shall not disassemble instrument without permission Only qualified professionals are allowed to replace elements or adjust the machine inside Replacement of elements under energizing state shall be prohibited c Use of power supply Before turning on AC power make the power voltage consistent with the instrument required one a 10 deviation is allowed Make the rated load for power socket not below instrument s maximum load d Power wire Generally this instrument uses accompanied power wire The damaged power wire shall be replaced by one of the same type and the same specification It can t be repaired The power wire shall neither be compressed nor be laid in gathered personnel location e Insertion and withdrawal of power wire At insertion and withdrawal of power wire the plug shall be correctly held with hand At insertion
25. USB root directory in fixed directory 3 Each user is the next subfolder under bieer pcrex file 4 Don t plug USB flash memory in out quickly To protect device and USB flash memory from damage the device will terminate the USB function until next run if USB flash memory is plugged in out quickly 5 The operation for dual module is same It only connects USB flash memory at start interface of two modules 6 Suggest USB flash disk with famous brands such as KingSton SanDisk etc 7 USB flash disk file system format must be FAT32 8 Open the instrument first and then insert the USB flash disk 9 Less USB flash disk files and space faster the reading User s Manual of LifePro Thermal Cycler Connection to computer Chapter V Connection to computer 1 Software installation Click Setup exe in accompanied disc install the LifePro computer connection program which is able to control 30 LifePro instruments on one computer 2 Online setting LifePro is equipped with embedded online module inside and tools for setting are available 2 1 Tool software installation Step 1 click and run nead_setup exe Step 2 click Next 18 Setup Network Enabler Administrator aaa ie Setup Network Enabler Administrator Select Destination Location Welcome to the Network Enabler Where should Network Enabler Administrator be installed Administrator Setup Wizard oe stall Network Enable ecinindetretor Zal1100 your Gad Setu
26. ace ol USB flash memory connection lt Press cursor up down to select copy Interface of USB connection fail Hotlid off 2009 05 1 10 05 00 No disk connection Press F1 to Back Press Left Right Key to Previous Next Page USB file list Hotlid off 2009 05 1 10 05 00 Filet File2 Press Left Right Key to Previous Next Page Copy Hotlid off 2009 05 1 10 05 00 Local User1 User2 Press Left Right Key to Previous Next Page LifePro Thermal Cycler Instruction for operation file user lt Turn page by cursor left right key if user number is more than 30 lt Press View to see device user file list Hotlid off 2009 05 1 10 05 00 Local E Device file list Press Back to return to interface of IR device user list lt Press cursor up down key to select copy file lt Turn page by cursor left right key if user number is more than 30 f i Press Copy to output file Press Left Right Key to Previous Next Page lt If copy is correct it shows the dialog box below Back Copy File Saved If not it shows File output error Caution USB reads writes 500 users each user has 2 000 files 1 2 USB reads writes bieer pcrex file at
27. ameters Baud Rate c Parity None T Data Bits 8 r Stop Bits 1 X Flow Control None v Jf OK X Cancel Click OK click confirmation and apply the setting 4 Run Upgrade exe 5 Press open select upgrading program bin ofia computer 6 Press start the computer begins to upgrade software ofin instrument and the instrument screen prompts Upgrade nowbegin 7 The instrument prompts Upgrade finished when software upgrading is finished 8 Firmware upgrading is finished 9 Click remove device remove device mapping LifePro Thermal Cycler Firmware upgrading 10 Recover the configuration Open Network Enabler Administrator select configuration function N Network Enabler Administrator Configuration File Function Configuration View Help 222 eon Function Configuration 0 Network Enabler Module s Network Enabler Admit Configuratisq dk COM Mapping Monitor Port Monitor X9 IP Address Report configuration Message Log 0 Monitor Log 0 No Time Description 5 1 1 O Wow 2008 1 3 14 15 25 Double click network number searched in list column open property page and select Network LifePro Thermal Cycler Firmware upgrading Configuration amp Information AutoWaming IPAddress Report Password DigtallO SerialCMD Model perating Mode NE 41105 Basic Network Serial erating
28. and reliable in quality e Temperature can be changed as fast as 4 C second The speed to increase or decrease temperature can be set as per need of experiment Several modes for temperature control better compatibility of reagent Large LCD screen user friendly operation interface simple and easy operation Power off protection available programs can continue after power recovery Height and pressure of hot lid can be set for different reaction tubes which prevent reagent evaporation and pollution effectively Connect to ordinary computer by RJ45 standard interface which is easy for software upgrading 4 Use of LifePro Thermal Cycler Integrating a number of advanced technologies LifePro Thermal Cycler meets the demand of LifePro Thermal Cycler Introduction different PCR experiments and rank top in similar products LifePro Thermal Cycler is attached with 1 0 2 ml standard centrifugal tube 2 Strip of 8 tube 3 Strip of 12 tube 4 Full half apron plates LifePro Thermal Cycler Features Chapter II Features This chapter introduces the usage transportation storage conditions of the instrument as well as its basic parameters performance and functions 1 Normal working conditions Ambient temperature 10 C 30 C Relative humidity lt 70 Power supply 220V 50Hz Caution Before using the instrument please make sure the working condition meets the above requirements Especially the power line
29. ating Mode User s Manual of LifePro Thermal Cycler Connection to computer Operating Node 1 Port s Selected 1st port is Port 1 perating Mode TCP Server Mode JReal COM Mode TEP Server Mode TCP Server TEP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 ij TCP Alive Check Timeout 7 Select TCP Server Mode 0 99 min Inactivity Timeout Max Connection D 0 65535 ms Data Packing Optional Delimiter 1 0 ff Hex Delimiter 2 O ff Hex Force Tx Timeout 0 65535 ms SOK X Cancel Click OK to complete IP address setting Click the COM Mapping in function list Network Enabler Administrator COM Mapping File Function COM Mapping View Help COM Mapping 0 com Parameter P Address Report add target device remove target device Message Log 5 Monitor Log 0 No Time Description 1 2008 1 3 14 26 33 Found Network Enabler Module s 1 2 2008 1 3 14 35 18 Configuration Changed NE 4110S 00 90 8 10 E2 B7 3 2008 1 3 14 35 39 Configuration Changed NE 4110S 00 30 E8 10 E 2 7 4 2008 1 3 14 35 49 Configuration Changed NE 4110S 00 90 E8 10 E2 B7 B 2008 1 3 14 37 22 Configuration Changed NE 4110S 00 90 E8 10 E2 B7 Click the add target device button to add target device User s Manual of LifePro Thermal Cycler Connection to computer Ny Network Enabler Administrator COM Mapping Eile Function COM Mapping V
30. egment The figure shows that it cycles from segment 1 to segment 3 and cycles for 10 times lt Repeat to press Cycle key to add paragraphs maximum is 5 Press Delete to delete the current paragraph immediately Move the cursor up down to enter previous or next paragraph lt Press Save key to access the interface for file saving Press Back key to retum to the interface for file list Caution Meaning of parameters is seen in attachment2 Special caution 1 Please save the new edited PCR program before running or the file can t run 2 If Time is set as hot lid is closed automatically when module temperature control works Cycle1 x From to Cycle2 x From to Cycle5 X From to LifePro Thermal Cycler Instruction for operation Relation of segment and paragraph in the file Interface for file saving Press character key in this interface to input file name press confirmation key to move the cursor forward Select User and File Name by up down key Delete character by left_key move cursor by right key Press Caps Key to shift between capital and lower Hotlid off 2009 05 1 10 05 00 case At upper left on screen it indicates User PCR1 capital or lower case as shown in the figure ED File Name PCR1 Press Main key to return to main Tam interface Password lt Press Save key to save the file When Password Check doe file
31. er TCP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeout 0 99 min Select TCP Server Mod Inactivity Timeout Max Connection 0 65535 ms Data Packing Optional Delimiter 1 0 ff Hex Delimiter 2 0 ff Hex Force Tx Timeout 0 65535 ms J OK X Cancel Click OK to complete IP address setting Caution To select TCP Server Mode is not to upgrade but to connect 2 Way to upgrade instrument software 1 Press F1 on instrument and pPower on open instrument press F on insttrumentto access software upgrading preparation status and find Prepare to upgrade on screen 2 Run Network Enabler Administrator program It communicates through virtual device mapping while instrument upgrading N Hetwork Enabler Administrator Configuration zt File Function Configuration View Help Zan LOK Function Configuration 0 Network Enabler Module s search button py Network Enabler Admi No Model MAC Address IP Address Status Configuration fa Port Monitor XE IP Address Report configuration mapping lt gt Message Log 0 Monitor Log 0 No Time Description How 2008 1 3 14 15 25 3 Configuration Click search button softwarethe teel searches device automatically LifePro Thermal Cycler Firmware upgrading N Network Enabler Administrator COM Mapping File Functio
32. ience The main interface appears if no problem is found then you can edit view modify or delete the PCR amplification file LifePro Thermal Cycler Instruction for operation Chapter IV Instruction for operation This chapter explains how to edit view modify and delete the PCR amplification file how to run PCR amplification file and how to set the instrument system parameters in detail Warning If the instrument sound or display is abnormal after start or failure alarm and indication is shown during self testing please turn off the power immediately and contact the supplier Caution If the sample amount for test is less than countersink amount on module please distribute the sample test tube as uniform as possible In this way it ensures that the hot lid presses at the test tube top steadily at file running the module bears uniform load and the temperature change in test tubes keeps uniform Caution Turn the knob at anti clockwise direction as per diagram before covering the lid clockwise rotation for pressing anti clockwise rotation for loosing until a dang dang sound is heard Cover the lid and turn the knob at clockwise direction until a dang dang sound is heard and then turn another step When a dang dang sound is heard when knob is rotated at first regardless of clockwise or anti clockwise press the unlocking device by finger turn the knob at the opposite direction for two steps and then rotation kn
33. iew Help oomen Function COM Mapping 1 COM 2 Network Enabler Admi No Configuration as 92 168 0 245 Hi Performance FIFO Ena 8 1 Nor if COM Mapping Monitor Port Monitor Qi IP Address Report Description 2008 1 3 14 26 33 Found Network Enabler Module s 1 2008 1 3 14 35 18 Configuration Changed NE 4110S 00 30 E8 10 E2 B7 2008 1 3 14 35 39 Configuration Changed NE 4110S 00 30 E8 10 E2 B7 2008 1 3 14 35 49 Configuration Changed NE 4110S 00 30 E8 10 E2 B7 2008 1 3 14 37 22 Configuration Changed NE 4110S 00 90 E8 10 E2 B7 Double click the network number setting property mapping page it searched in list column and select Advanced Settings setting as shown below COM Port Settings Port Number 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Tx Mode Classical FIFO Enable Fast Flush Only Flush Local Buffer Perl fort ed Fo X Cancel Select Serial Parameters setting as shown below User s Manual of LifePro Thermal Cycler Connection to computer CON Port Settings Port Number 1 Part s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Baud Rate 9600 7 Parity None Data Bits s r Stop Bits 1 Flaw Control None T Jf OK X Cancel Click OK click confirmation apply the setting finally click remove device and complete mapping setting Caution 1 Several
34. ion introduction of Thermal Cycler and features of LifePro Thermal Cycler 1 Introduction of PCR technology Polymerase chain reaction PCR is a nucleic acid amplification that simulates natural DNA replication in vitro It takes 2 DNA chains to be amplified as template uses a pair of artificially synthesized oligonucleotide primer as mediator and amplifies certain DNA fragment quickly and specifically by thermostable DNA polymerase Ever since PCR technology was invented by Mullis in 1985 and thermostable DNA polymerase was discovered by Erlich in 1988 this simple fast specific and sensitive technology has been widely applied to life science areas with astonishing speed Remarkable achievements have been witnessed in cytology virology oncology genetic disease forensic medicine animal plant immunology and etc It is a milestone in contemporary molecular biology development 2 Example of PCR technology application scope Research gene clone DNA sequencing mutation analysis gene recombination and fusion protein regulating DNA sequence identification transposition insertion site mapping genetic modification detection synthetic gene construction clone construction carrier expression certain gene s incision enzyme diversity detection and etc Clinical diagnosis bacteria spirochete mycoplasma Chlamydia mycobacterium rickettsia diphtheria bacillus pathogenic E Coli dysentery bacillus aeromonas hydrophilic and C difficile
35. l be flashing during overshoot LifePro Thermal Cycler Instruction for operation 1 2 4 Hotlid indicates the on off status of hot lid 1 2 5 Key Sound and Run End Sound indicate the sound to press key and the sound at program finish respectively See attachment 2 Value and Meaning of Parameter Setting of this manual for specific index of each parameter above Caution 1 Press Save to confirm the system parameter setting or the system parameter won t change 2 It calibrates the clock inside the instrument as per Beijing time upon ex factory 3 Itis recommended to check the date and time again for the first time to use 1 3 Gradient module temperature distribution features The gradient module uses 3 line temperature control unit The gradient temperature is distributed as curve as shown in figure below the set temperature is 40 C and the set gradient temperature is 20 C The temperature gradient in each hole row on module is nor uniform The temperature difference on hole rows at center is higher than the that at outside Therefore at gradient module operation the temperature of each hole row shown on instrument governs Gradient graphs 60 58 56 54 52 50 48 46 44 42 40 Temperature C 1 2 3 4 5 6 7 8 9 10 11 12 Column left to right 2 How to input output PCR program 2 1 Connect to USB flash memory USB connection interface 2009 05 1 10 05 00 Press UsbLink
36. ler Administrator on your computer Completing the Network Enabler Administrator Setup Wizard Setup has finished installing Network Enabler Administrator on your computer The application may be launched by selecting the installed icons Extracting files C Program Files Network Enabler Administrator NEADMIN exe CT Click Finish to exit Setup Cancel 2 2 Device configuration 2 2 1 Connect the device to computer by network cable cross B type connection network cable compute device User s Manual of LifePro Thermal Cycler Connection to computer 2 2 2 Run Network Enabler Administrator N Network Enabler pee wees guration Eie Function Configus Search button g E Sn Function as ation 0 Network Enabler Module s 7S Network Enabler Admit Na W configu ration ks IP Address Status Configuratie dif COM Mapping Monitor Port Monitor X9 IP Address Report mapping Message Log 0 Monitor Log 0 No Time eescription Now 2008 1 3 14 15 25 2 2 3 Configuration Click the search button and the tool searches instrument automatically v Network Enabler Administrator COM Mapping File Function COM Mapping View Help POB on Function COM Mapping 1 COM Ba Network Enabler Admi No Configuration NE 41105 192 245 Hi Pe
37. lid off Date Time Default User Default File 2009 05 1 10 05 00 Control Mode Hotlid Key Sound Run End Sound Yes Back a Block mode only concerns the temperature control for module during temperature rise fall Now that the temperature rise fall of reagent lags behind the module the actual LifePro Thermal Cycler Instruction for operation temperature change of reagent is different from the program we set The actual time that the reagent reaches the set temperature is far less than the set time see figure below for relation between module temperature and reagent temperature module reagent o ni g z p amp E g b Tube mode considers the temperature rise fall of reagent that lags behind the module There is an overshoot when it reaches the set temperature so that the reagent reaches the set temperature faster At the set time same with Block mode obviously the actual time that reagent reaches set temperature is prolonged See figure below for relation between module temperature and reagent temperature 100 bia module 94 Ea 91 g s reagent amp os i 2 2r 5 1 2a a P 79 y z 76 E 73 70 Note In the tube mode timing will start after overshoot is finished and the temperature wil
38. lock mem Default User BIOER Default File PCR1 Select Sample Volume Back Run 1 1 How to edit PCR program Interface for file list Press File in main interface to access the interface for file list As shown in figure there Hotlid off n 2009 05 1 10 05 00 are 3 files in the file list Use the up down key No User File Name Save Time to select user when blinking bar blinks in the BIOER1 PCR1 2009 05 1 User column and the file for selected user is BIOER2 PCR2 2009 05 1 shown in the File Name column shift to File BIOER3 PCR3 2009 05 1 Name by right click then the blinking bar blinks in the File Name column select corresponding ra file by up down key Press Edit to edit the file pointed by cursor lt Press New File to edit a new file Press Delete it asks Confirm delete file select Delete or Back as per information it New File Delete lt Press Run to confirm running mode and test tube type if file password is not asked press Run to bounce the dialog box below if file password is asked Input Password Press Run to access the interface for file running when Control Mode is Block press Run to bounce the dialog box below if Control Mode is Tube now Select Sample Volume 050ul It is the same with main interface confirm the password input the sample type and micro liter I LifePro Thermal Cycler Instr
39. mum length 11 D Default user name 11 characters in efault User in system maximum length In this mode it comes to constant temperature status once it reaches Block target temperature the time that reagent temperature at target temperature is less than Temperature time set see Fig 1 12 Control Mode In this mode it comes to control mode constant temperature status after 3 C overshoot once it Tube reaches target temperature the reagent temperature approaches target temperature quite fast see Fig 2 13 Sample Volume Sample size ul 10 200 LifePro Thermal Cycler Attachment 2 Hot lid off Off 14 Holid f Hot lid on C 30 110 15 Key Sound Key tone yes no Yes No Buzz Prompt tone at 16 Run End Sound running finish Yes No Continuous buzz yes no Temperature C Temperature C Note In the tube mode timing will start after overshoot is finished and the temperature will be flashing during overshoot Memorandum
40. n COM Mapping View Help OOOO A Function ll s Network Enabler Admit No COM Mapping 1 COM IP Address Parameter Hi Performance FIFO Ena NE 41105 192 168 0 245 e Configuration dk COM Mapping Monitor Port Monitor X IP Address Report Network device number which isit searched Description 9600 None 8 1 Nor Double click the network number searched on list column open the property page and select network Configuration fs boo Auto Warming IP Address Report HE i i Operating Mode NE 41105 Basic Network Serial perating MAC Address vw Modify 00 90 8 10 E2 B7 Pot Alias OP Mode sm COM Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver2 1 Status Data Mode Password DigtallO SerialCMD Accessible IPs tting Settings so Click the Modify check box to modify configuration X Cancel Open the property page select Operating Mode and click settings LifePro Thermal Cycler Firmware upgrading Configuration amp Information Auto Waming IPAddress Report Password Digitall0 SerialCMD Model Operating Mode NE 41105 Basic Network Serial perating Accessible IPs MAC Address v Modify 00 90 8 10 E2 B7 7 7 hReal COM Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Settings Click the Modify check box to modify co
41. nfiguration Jf OK x Cancel Select Real com Mode under Operating Mode choices Operating Mode 1 Port s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode em TCP Server Made TCP Server TCP Client Made UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeou p M Select Real Com Mode Inactivity Timeout Max Connection 0 0 65535 ms Data Packing Optional Delimiter 1 0 ff Hex Delimiter 2 0 ff Hex Force Tx Timeout 0 65535 ms J OK X Cancel Caution To select Real Com Mode is not to connect but to upgrade Click COM MAPPING in function list LifePro Thermal Cycler Firmware upgrading N Network Enabler Administrator COM Mapping File Function COM Mapping View Help com Mapping 0 COM add target device Pat Monto P Address Report remove target device Found Network Enabler Module s 1 Configuration Changed NE 41105 00 90 E8 10 E2 B7 Configuration Changed NE 41105 00 90 E8 10 E2 B7 Configuration Changed NE 41105 00 90 E8 10 E2 B 7 Configuration Changed NE 41105 00 90 E8 10 E2 B7 E COM Mapping 0 COM Network Enabler Admi No Model IPAddess Pot COMPot Mode Parameter Configuration I 4 COM Mapping Monitor Port Monitor IP Address Report Found Network Enabler Module s 1
42. ob on unlocking device can be released 1 How to run PCR program Power on the thermal cycler gives a du du sound which indicates the power supply is available Self testing is shown on screen and the instrument takes about 1 to 2 minutes for self testing Please wait with patience The main interface appears if no problem is found me Main interface Shown on the upper left of main interface is the status of hot lid setting Hotlid off 2009 05 1 10 05 00 Shown in the middle of main interface is the information such as the Bioer information software version module type default BIOER program default user control mode sample amount and so on egene rn Press File to access the interface for Block B 96G file list Default User BIOER Press System to access the interface Default File PCR1 for system parameter setting Control Mode Tube lt Press Run to confirm running mode and test tube type if file password is nol asked press Run to bounce the interface below if file password is asked UsbLink System Press number key to input the password lt Press Back to return to main interface lt Press Accept to access the interface for file running if Control Mode is Block now press Accept to bounce the dialog box for test tube volume setting if Control Mode is Tube now LifePro Thermal Cycler Instruction for operation
43. one GMT Greenwich Mean Time Dublin Edinbur Status Data Mode Local Date 2000 1 1 v Local Time 0 30 22 Click the Modify check box to modify configuration Jf OK X Cancel To click Network the following interface will appear LifePro Thermal Cycler Firmware upgrading Configuration Information Auto Warning Model Network NE 41105 MAC Address CY Mody 192 168 0 250 00 90 E8 1C AF 60 IP Address Cewa D Serial Number 5824 Netmask Gateway Firmware Ver Ver 4 1 IP Configuration Giic gt DNS Server 1 IP Address Repot Password DigtallO SerialCMD Advanced Network Serial Operating Mode Accessible IPs BIOS Ver Ver 2 1 DNS Server 2 Status Modify Data Mode Click the Modily check box to modify configuration J OK X Cancel 3 Configuration To click search button software will search device automatically N Network Enabler Administrator COM Mapping File Function COM Mapping View Help oomen Function COM Mapping 1 COM E el Network Enabler Admit No IP Address Parameter Configuration NE 41105 192 168 0 245 Hi Perfmanee FIFO Ena S600 None 8 tk COM Mapping Port Moror p instrument network number which isit searched Message Log Monitor Log 0 No Time Description Double click the network number in the list column which is searched open the property page and select network items Life
44. p will install Network Enabler Administrator into the following folder Itis tocormensed that you close all other applications before To continue click Next If you would like to select a different folder click Browse continuing Browse Click Next to continue or Cancel to exit Setup Atleast 2 5 MB of free disk space is required lt Back Next gt Cancel Step 3 select destination location click Next Step 4 Click Next z B Setup Network Enabler Administrator fo Setup Network Enabler Administrator Select Additional Tasks Ready to Install Which additional tasks should be performed Setup is now ready to begin installing Network Enabler Administrator on your computer Select the additional tasks you would like Setup to perform while installing Network Click Install to continue with the installation or click Back if you want to review or Enabler Administrator then click Next change any settings Additional icons Destination location C Program Files Network Enabler Administrator Additional tasks Additional icons Create a desktop icon lt Back Next gt Cancel wm Install A i User s Manual of LifePro Thermal Cycler Connection to computer Step 5 click Install Step 6 complete installation click Finish iB Setup Network Enabler Administrator E x iB Setup Network Enabler Administrator Installing Please wait while Setup installs Network Enab
45. rformance FIFO Ena dk COM Mapping Monitor Port Monitor XQ IP Address Report instrument network number it searched Description Double click the network number in the list column that it searched open the property page and select network items User s Manual of LifePro Thermal Cycler Connection to computer Configuration Information Auto Waming IPAddress Repot Password Digitall0 Serial CMD Model NE 41105 Basic Network Serial OperatingMode lt AccessibleIPs v Modify MAC Address IP Address 192 158 0 245 00 90 8 10 2 B7 input IP address Serial Number n 3898 Netmask 255 255 255 0 Gateway input subnet mark Firmware Ver Ver 4 0 IP Configuration Static DNS Server 1 BIOS Ver Ver 2 1 DNS Server 2 Status Modify Data Mode Click the Modify check box to modify configuration wv OK X Cancel Open the property page select the Operating Mode items click the settings button Configuration I3 ies cad Auto Warning IP Address Report Password Digital 10 Serial CMD E i i Operating Mode i NE 41105 Basic Network Serial perating Accessible IPs MAC Address iv Modify 00 90 E8 10 E2 B7 OP Mode AI Rea COM Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Settings Click the Modify check box to modify configuration w OK x Cancel Select TCP Server Mode under Oper
46. ry then select Configure from menu of right button Network Enabler Administrator Configuration File Function Configuration View Help Configura on E Network Enabler Module s LNNNIT NM MAC Addr IP Address ic AX H NE 41105 00 30 E8 11 AF 60 192 169 0250 FA Network Enabler Admit Configuration dh COM Mapping Monitor Port Monitor XX IP Address Report LifePro Thermal Cycler Firmware upgrading N Network Enabler Administrator Configuration n File Function eae View Help 222 LOR ATERT IB Network Enabler Admit Configuration tig COM Mapping mm r Broadcast Search Monitor 2 Specify by IP Address Port Monitor X IP Address Report 25 Locate 72 Unlock E Configure E Upgrade Firmware Export Configuration Import Configuration Assign IP Address Then appear the following setup window Configuration 1 Network Enabler Module s Model MAC Address IP Address Status 1 NE 41105 00 30 E8 1C AF 60 192 168 0 250 Configuration Information ming IPAddress Repot Password Digitall0 SerialCMD pe 105 Network Advanced Network Serial Operating Mode Accessible IPs MAC Address Modify 00 90 E8 1C AF 60 Device Name NE 41105_5824 Serial Number 5824 L Modify Ena Firmware Ver Es v Enable Telnet Console Ver 4 1 BIOS Ver Ver 2 1 Modify Time Z
47. s Reaction tube is not on reaction tube try placed uniformly to place symmetrically Please cover Reaction tube is not reaction tube tightly covered tightly before put into instrument It didn t press Save to Please refer to 1 2 13 System parameter setting is invalid confirm system parameter of Chapter IV in this setting manual Caution 1 It is forbidden for user to open the case and check by oneself in warranty period Please contact supplier or manufacturer in time if any failure needs to open the case to check 2 Since temperature control characteristics rate of temperature rise fall stability fluctuation and etc of different brands and each instrument are specific and biotic experiment itself has uncertainty and vulnerable to outside influence PCR program that runs well on one instrument might fail to reach same effect on another Therefore please adjust PCR running program to get ideal performance once instrument is changed LifePro Thermal Cycler Attachment1 Attachment 1 Wiring diagram This diagram is for reference only and subjected to change without notice
48. ss Run to confirm running mode and test tube type if file password is nol asked press Run to bounce the interface below if file password is asked UsbLink System Press number key to input the password lt Press Back to return to main interface lt Press Accept to access the interface for file running if Control Mode is Block now press Accept to bounce the dialog box for test tube volume setting if Control Mode is Tube now Hotlid off Press to select the test tube type in this interface Press Back to return to main interface BIOER Press Next to access the interface for file running if Control Mode is Block now press Bioer Technology Co Ltd Next to bounce the interface below il Version 2008 1 1 Control Mode is in Tube mode now Block RAKES Default User BIOER Default File PCR1 Control Mode Tube LifePro Thermal Cycler Instruction for operation Select Tube Type 0 2ml Press Character key to input the micro liter Hotlid off 2009 05 110 05 00 amount of sample size Different sample sizes have different overshoot temperature and lasting time when temperature control mode is Tube BIOER lt Press Back to return to main interface Press Run to access the interface for Bioer Technology Co Ltd Version 2008 1 1 file running B
49. the goods against the packing list In case of damaged or missed goods please immediately contact the supplier After qualification of acceptance properly fill the product acceptance sheet and send or fax the copied sheet to the supplier for filing and maintenance After unpacking the packing box and packing materials shall be well kept for future repair For the damage of instrument occurring during transporting to the repair department due to improper package Hangzhou Bioer Technology Co Ltd shall bear no liability User s Manual of LifePro Thermal Cycler Content CONTENT User s Manual of LifePro Thermal Cycler Content EE NNN T 1 Software installation ce eccecccccesceccecsececesececeseceusasecessecssaeevsseeseseesessessesesesisesesisesusssssurseesuisessuisesersseseuesees 17 PES ERTER 17 2 1 Tool software InstallatlODi icc eiat rn re E pna cea saca eu aaa ce era se frites 17 2 PE congre oc ciuis escuche Lu iD LI MIDI LED LL LA 18 2 2 2 Run Network Enabler Administrator rtt ettet einst oen ker nas 1918 PE EET 19 Attachment 2 Value and Meaning of Parameter Setting srsrrrrrrrnresnrrsvrrsvnrsnvnrsvnrsvnssvvnsvvnssvassvvssvvnenen 49 LifePro Thermal Cycler Introduction Chapter I Introduction The contents in this chapter include introduction of PCR technology examples of PCR applicat
50. trument Caution In any of the following cases immediately cut off the power supply withdraw the power plug from the power socket contact the supplier or ask qualified repair personnel for treatment Liquid drops into inside of the instrument The instrument is rained or watered The instrument works abnormally especially generates abnormal sound or odor e The instrument is dropped or its casing is damaged The function of instrument obviously changes 3 Instrument label 1 HOT SURFACE Warning The instrument is adhered with mark HOT SURFACE During operation of instrument or a period immediately after completion of running of a program any portion of body shall not be contacted with metallic portion module or hot lid nearby the said mark otherwise skin would be burnt 4 Maintenance of instrument In order to ensure full contact and good heat conduction between the test tube and the conical holes the conical hole on the module shall be regularly cleaned with clean soft cloth soaked with a little absolute alcohol The stained surface of this instrument if any shall be cleaned with soft cloth soaked with cleaning paste Warning At cleaning of instrument its power supply shall be cut off The instrument surface shall not be cleaned with corrosive cleaning agent 5 After sales service The warranty content and scope are shown in the warranty sheet Note After unpacking immediately check
51. ts click Save to save file Prompt All steps can be viewed from graphs User must input password to edit file if password is required by file Id GenePro e x 28 3 8 RUDLDRARLLH T 5 e ai Bg Tav Computer gt 1 gt 1 a Fee af Bi Temp Tine 10 00 50 00 01 00 01 00 01 00 01 00 01 00 10 00 End Segment Cycle Times 10 Begin Segment 1 5 Seg04 72 00 am Seg 2 55 00 Seg03 800 01 00 Seg 6 55 00 50 00 01 00 01 00 setting details Select the file to be copied Select Copy File menu under File menu User s Manual of LifePro Thermal Cycler Connection to computer aste file Select User item under device list File can t be overlaid if it is already existed and open Prompt File is only copied and pasted between computer and device File can t be overlaid if it is already existed and open Delete file Select file to be deleted Select Delete File menu under File menu Prompt File can t be deleted if it is open Change file name Select file to change name Select Change File Name menu under File menu Input file name and click OK Prompt File name can t be changed if it is open Change File Name Do you want to change file name of Computer gt TTTT gt 1 Enter new file name The file name can contain only letters and numbers w OK x Cancel Change file password
52. uction for operation amount of sample size Note 1 on upper right of user and file indicates this file is the default for system running 2 File can be modified in the interface for system parameter setting Interface for file editing Segment and paragraph can be edited in this Hotlid off 20097 1105 4 interface The file is comprised of segments 4n nzlnn n and paragraphs while segments are contained in paragraphs Cycle times can be set for segments in paragraphs see figure below for relation Press up down or left right key to move the cursor the place where cursor is are highlighted Press character key to change the parameter setting Press plus minus key to change the Seg Temp C Time Ramp C s Temp Time Grad plus minus symbol 02 Press Seg key for segment editing 02 00 0 0 40 00 02 where you can set temperature Temp 72 0 01 45 0 0 0 00 02 lasting time Time rate for temperature 04 0 00 00 0 0 0 00 00 rise fall Ramp temperature increment in each cycle Temp and time Cycle1 x10 From01to03 Press Enter To View Delails increment in each cycle Time and Qan l tuna 11 Nalata Rank 11 Cava gradient temperature difference between the left line at 55C to the right line at 570 in each cycle Grad Press Delete to delete the segment where cursor is lt Press Cycle key for paragraph editing where you can set cycle amount and cycle starting s
53. ype Click Calc User File Tools Help KL KLAS 3 2 32 amp iz 3 31d 8 8 es a _ Grad Temp a mee amp B 34 Temp 30 Gradient 24 Colder Hotter Change device IP and device name Select Server Setting item under Tool menu to bounce the dialog box for setting Click Add to add line Click Delete to delete current line Fill in IP address and alias click Save to save setting Prompt The setting comes into valid only after software restart P setting must match the device User s Manual of LifePro Thermal Cycler Server Setting 192 188 0 222 192 188 0 243 192 188 0 240 192 168 0 241 per001 per003 per004 Connection to computer GAdd t Delete td Save Cf Close LifePro Thermal Cycler Firmware upgrading Chapter VI Firmware upgrading 1 Device configuration 1 Connect device to computer by network cable gray cross B type cennection PUSH network cable compute device 2 Run Network Enable Administrator To affirm state of confiquration in the column of Function N Network Enabler Administrat File Function Configuration View Help 222 LEOR Function Network Enabler Admit No 6 dif COM Mapping Monitor Port Monitor XX IP Address Report Caution If there is no the column of Function please select View gt Function Panel then displa the column of Function
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