Hardware User`s Manual Automated place preference box – Black
Contents
1. Figure 16 Placing the floors in the cage Automated place preference box Black and white 17 Panlab From the front side of the cage insert the set of floor and platform done in point 9 1 and match the 4 legs of the platforms with the 4 holes we find inside the cage These supports in grey and black compartments transmit the weight to the load cell located under the cage 9 3 ASSEMBLING THE DOORS Central compartment doors are inserted from above and slide down helped by a guide Mounting or not these doors can change the configuration of the cage 9 4 ASSEMBLING FRONT COVERS In black and white compartments the front cover is fixed and transparent it has in the top two metal pegs for hanging a translucent smoked lid In the case of the central compartment the cover is inserted like the doors by inserting it from above and lowering it through the guide The cover can be transparent or translucent smoked The rear panel of this compartment is grey opaque and again is inserted through the guide from the top Figure 17 Two examples of different configurations of front covers Automated place preference box Black and white 18 Panlab 10 WORKING WITH THE EQUIPMENT 10 1 RUNNING AN EXPERIMENT The following steps must be followed to work with the unit 1 Connect equipment as shown in chapter 8 2 Configure boxes with accessories front covers floors as shown i2. MOUSE When working with mice cage LE 893 e POWER Power inlet main switch and fuse holder Automated place preference box Black and white 11 Panlab Lan Harvard Apparatus 7 3 EXPERIMENTATION CAGE WHITE GRAY BLACK COMPARTMENT COMPARTMENT COMPARTMENT NL ES Figure 8 Experimentation cage front view The experimentation cage has three compartments The two on either side are larger The left one is white and the right one is black The central compartment is smaller and grey It features two doors to communicate with the side compartments r a Figure 9 Central compartment doors in white and black side seen from both faces The front panel of the three compartments is transparent but can be placed in them a smoked translucent cover In the case of the central compartment is sliding the front panel and can be transparent or smoked Figure 10 Central compartment smoked translucent and transparent cover Automated place preference box Black and white 12 Panlab The animal s position is detected by load cells Only the grey and black compartments have load cells under the floor When the system does not detect the animal in the grey or the black compartment it is assumed that the animal is in the white compartment The platforms have legs that rest on four supports which transmit the weight to the sensor located under the cage platform Figure 11 Platforms of lateral compartm
3. The program used to control the system is PPCWin Some points have to be taken into account when connecting more than one control unit to the computer Automated place preference box Black and white 15 n la b Apparatus e All control units must have a different identifier ID so that the computer can correctly identify them e tis not necessary for all ID numbers to be consecutive For example a system with 3 cages can work with IDs 1 2 3 or 4 6 8 e t is not necessary for control units to be physically connected in order according to their ID numbers For example a system with three units 1 2 and 3 could be connected in the following combinations 1 2 3 1 3 2 2 1 3 2 3 1 3 1 2 and 3 2 1 e The computer serial port is connected to the MAIN port of the first unit e REMOTE port of each control unit is connected to the MAIN port of the next control unit e The REMOTE port of the last unit is left free Automated place preference box Black and white 16 Panlab Hever 9 CAGE ASSEMBLY 9 1 ASSEMBLING THE FLOORS The floors of the left and right areas White and Black can be smooth or rough simply turning the ceiling which makes the function of floor Figure 15 Floors and platforms of white black and grey compartments To fit the platforms and floors you must fit the hole in the floor with the platform pivot 9 2 PLACING FLOORS IN THE CAGE
4. preference box Black and white 25 Panlab towers 6 Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment gt lt Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en e
5. ENTIONS EXPERIMENTATION CAGE MODEL ANIMAL SIZE WEIGHT Length Width Height EUM Dimentions are expressed in milimetres Automated place preference box Black and white 24 Panlab Modes ir DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direcci n del fabricante Energ a 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto PLACE PREFERENCE Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 892 LE 893 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 08 EC Directiva EMC Directive Directive 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Res
6. Hardware User s Manual Automated place preference box Black and white lt References LE892 76 0218 LE893 76 0219 Version V27 10 2014 Panlab s l u International Calls 34 934 750 697 Pa n b H A Harvard C Energ a 112 Domestic Call 934 190 709 APPORINUS 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Spain Info panlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab pb 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be N exposed to a hazard Protection terminal ground connection Warning about a hot surface which temperature may exceed 65 Warning about a metal surface that can supply electrical shock when it s touched Decontamination of equipments prior to disposal at the end of their operative life Waste Electrical and Electron
7. d when control unit is turned on the ready led will start flashing indicating that the system is auto balancing But after 20 seconds the two red leds on the right side will flash too This is an error message indicating that the system will not work To solve this problem proceed as follows e Turn off the control unit e Check that the control unit and experimentation cage are correctly connected e Checkthatthe floors are correctly placed in their supports e Turn control unit e Wait around 20 seconds while system is auto balancing If the error message still appears it means the load cells are damaged 10 3 CLEANING THE FLOORS To clean the floors you should use a wet cloth and then you can dry it with a dry cloth 10 4 CLEANING THE DOORS To clean the doors you should use a wet cloth and then you can dry it with a dry cloth 10 5 CLEANING THE TRANSPARENT AND SMOKED COVERS To clean the floors you should use a wet cloth and then you can dry it with a dry cloth WARNING For this accessories never use alcohol or alcohol derived products otherwise stripes will appear in the transparent plastic Automated place preference box Black and white 20 Panlab 11 TROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION The equipment does not start up Check the condition of the fuses Ensure that the cable DB25 to DIN7 connects the cage and c
8. electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instru es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribui o para a protec o do ambiente I lt Automated place preference box Black and white 26
9. ents and the central compartment The floors of the black and white sides can be smooth or rough while the central compartment is just smooth Figure 12 Floors of compartments white grey and black On the left side of the platform there is a DIN 7 female connector It is used to connect the experimentation cage to the control unit Automated place preference box Black and white 13 8 EQUIPMENT CONNECTION Figure 13 shows the necessary connections a se ND x am v am a J 000000000000 Rated Voltage 90 260 V 50 60 Hz 18VA Made in Spain by Panlab 5 1 Figure 13 Equipment connection Automated place preference box Black and white 14 Harvard n la b Apparatus The necessary cables and connections are listed in the next table FROM CABLE LE 89X MAIN Computer COM port LE89XBox Cage platform DB25 to DIN7 A computer can control up to eight cages with control units connected in cascade Box ld E Remote e Were DE Mouse 00000000000 Rated Voltage 90 260 V AC 50 80 Hz 18VA J Aaa Rated Voltage 90 260 V AC 50 60 Hz 18VA 000000000 Ves Rated Voltage 90 260 V 50 60 Hz 18 Made Spain by 1 Figure 14 Example of connection of 3 cages Figure 14 shows an example of how three control units would be connected
10. equired to correct the interference at his own expense Automated place preference box Black and white Panlab 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment check fuses according to the following procedure 1 Remove power cord from the power inlet Automated place preference box Black and white 2 Open fuse holder pulling the flange with a regular screwdriver 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct p
11. ic Equipment Directive WEEE WE 2 GOODLABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Automated place preference box Black and white Panlab 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A Due to the weight of the cage and its dimensions it should be moved between two persons in order to avoid possible damage when moving it B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all tra
12. inuously on indicating that the equipment is ready to work e POSITION LEDS there are three 5 mm red leds one for each compartment of the cage These leds remain on while the animal is detected in the respective compartment Automated place preference box Black and white 10 Panlab pb 7 2 CONTROL UNIT REAR PANEL MAIN ID REMOTE RAT MOUSE BOX Rated Voltage 90 260 50 60 Hz 18VA Made in Spain by Panlab s l Figure 7 Control Unit Rear Panel e MAIN DBg female port It is used to connect the control unit to the computer serial port if the control unit is first or to connect the control unit to the REMOTE port of the previous control unit e REMOTE DBg male port It is used to connect the control unit to the MAIN port of next control unit when there are several units If the control unit is the last one this port is left free e ID Decimal selector used to identify the control unit A computer is able to control up to 8 control units with the PPCWin software All connected cages must have a different identifier so that computer can identify them e BOX DB25 connector it is used to connect the control unit to the experimentation cage The control unit receives information from the cage load cells through this connector to identify where the animal is e RAT MOUSE This switch must be set in the correct position depending on the box used When working with rat cage LE 892 o
13. ite Panlab pb PC Control Some of these instruments are designed to be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and standards and 15 set up accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Class A equipment is intended for use in an industrial environment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference N when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if WARNING not installed and used in accordance with these instructions may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be r
14. l embalaje hace referencia ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de aparatos usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente gt lt y Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique 5 Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das je
15. n chapter 9 3 If you work with the program PPCWin and several control je units choose a different ID for each cage 5 O 4 If working with the rat cage LE892 select Rat with the pa selector on the rear panel If working with the mice cage LE893 select Mouse with the same selector 5 Connect the control unit to the AC network and turn it on 6 Wait few seconds until the led remains continuously on It will flash during auto calibration 7 The position led of left compartment will turn on although there is not animal in the cage the system only has two sensors if the animal is not detected by any of these sensors the system assumes that the animal is in the compartment without sensor 8 Follow the PPCWin user s manual instructions to prepare the experiment 9 Place animal in the experimentation cage and run the experiment 10 Once the experiment has ended remove animal from experimentation cage clean the cage and return to step 8 if the experiment is to be repeated with more animals 11 Once the experimental session has ended turn off the control units exit from PPCWin software and clean the experimental cages Automated place preference box Black and white 19 Panlab 10 2 ERROR MESSAGE If the control unit is not correctly connected to the experimentation cage or the floors have not been correctly placed in their supports or if one or both load cells are damage
16. ndard experimental chamber used to evaluate rodents preference of conditioned place This is a test commonly used to detect medicine reinforcement properties or natural stimulus It is also used to research the brain neurobiology involved in reward and addiction Figure 5 Place Preference e t features three Perspex compartments two side compartments one black and the other white and a central grey compartment with connection doors featuring manually activated sliding doors e Smooth and rough floors are supplied for the white and black compartments e Front panels are transparent but removable smoked translucent lids are also supplied Experimentation cages are supplied with an automatic animal position detection system Animal position is detected by means of load cells transducers installed below the cage platform Cages with automatic position detection are controlled with PPCWin software Automated place preference box Black and white Panlab 7 EQUIPMENT DESCRIPTION 7 1 CONTROL UNIT FRONT PANEL READY LED POSITION LEDS PLACE PREFERENCE LE 892 893 4 N O Panlab HARVARD APPARAT US Figure 6 Control unit front panel e READY LED 3 mm green led When the control unit is turned and the cage is connected to it this led will flash for about 20 seconds indicating that the system is completing the auto balance procedure Once the sensors are balanced this led will remain cont
17. nsport locks are removed before use The original packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category ll over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Automated place preference box Black and wh
18. ontrol unit Check that the floors are correctly The two right side red position leds are placed blinking If the cable is connected and all correctly placed this means both load cells are damaged The load cell 15 damaged One of the red position leds placed to the blocked or the floor is not correctly right is blinking placed Check that Ready led is on Check that floors are correctly placed Check that the RAT MOUSE switch is in the correct position Rat weight threshold detection is 3ogr if you work with mice the equipment will not be able to detect them The control unit does not detect animal position Make sure your equipment is connected to PC via RS 232 Check that PPCWin settings so that the serial port is correct Restart the equipment and the PC to do a RESET in communications Check in the rear panel of control unit that the ID number is the same that the one set in PPCWin If you work with more than one control unit check that all the ID numbers are different so that the computer is able to distinguish all cages The equipment does not communicate with PPCWin Automated place preference box Black and white 21 Panlab This is not an error the system only has two sensors one in the corridor and the other one in the black compartment if the animal is not detected by any of these sensors the system assumes that the animal is in the compartment without sensor the whi
19. osition CORRECT INCORRECT Figure 4 Fuses position 5 Insert again fuse holder both possible positions are correct because power supply is universal 6 Ifthe fuses blow again unplug the equipment and contact technical service For electrical safety never open the equipment The power supply has dangerous voltages WARNING Automated place preference box Black and white Panlab 5 TABLE OF CONTENTS SYMBOLS TABLE 3 2 GOOD LABORATORY PRACTICE 3 UNPACKING AND EQUIPMENT INSTALATION 4 MAINTENANCE 5 TABLE OF CONTENTS 6 INTRODUCTION 7 EQUIPMENT DESCRIPTION 7 4 CONTROL UNIT FRONT PANEL 7 2 CONTROL UNIT REAR PANEL 7 3 EXPERIMENTATION CAGE 8 EQUIPMENT CONNECTION 9 CAGE ASSEMBLY 9 1 ASSEMBLING THE FLOORS 9 2 PLACING FLOORS IN THE CAGE 9 3 ASSEMBLING THE DOORS 9 4 ASSEMBLING FRONT COVERS 10 WORKING WITH THE EQUIPMENT 10 1 RUNNING AN EXPERIMENT 10 2 ERROR MESSAGE 10 3 CLEANING THE FLOORS 10 4 CLEANING THE DOORS 10 5 CLEANING THE TRANSPARENT AND SMOKED COVERS 11 TROUBLESHOOTING Automated place preference box Black and white Harvard Apparatus 10 10 11 12 14 17 17 17 18 18 19 19 20 20 20 20 21 Panlab pd 12 PREVENTIVE MAINTENANCE 23 13 TECHNICAL SPECIFICATIONS 24 Automated place preference box Black and white Harvard A Apparatus Panlab 6 INTRODUCTION Panlab s Standard Place Preference is a sta
20. te one Although there is no animal placed in the cage the left red position led in the control unit is on indicating that the animal is in the white compartment Automated place preference box Black and white 22 Panlab 12 PREVENTIVE MAINTENANCE EXPERIMENT CLEANING THE FLOORS CLEANING THE DOORS CLEANING THE TRANSPARENT SMOKED COVERS Automated place preference box Black and white 23 Panlab pb 13 TECHNICAL SPECIFICATIONS POWER SUPPLY Input voltage Frequency Fuse Maximum power Conducted noise Universal 100 240 VAC 50 60 Hz 2 fuses 5x2omm 2A 250V Fast 18 W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity 10 C to 40 C 0 to 85 RH non condensing Storage temperature 09 to 50 C non condensing POSITION DETECTION Rat 30 gr Mouse 7 gr CONNECTOR MAIN REMOTE Pin Function 2 Rxd 3 Txd 5 Gnd 7 Rts 9 Cts COMUNICATIONS OUTPUT Standard Interface RS232C Connector Transmission speed Delta 9 contacts connector 19200 bauds 8 bits no parity CONNECTOR BOX Pin 10 11 12 13 23 24 25 Function Transducer left S Transducer left S Transducer Right 5 Transducer Right S Transducer Exc 10V Transducer Exc Gnd DIMENTIONS CONTROL UNIT Width x Height x Depth Weight 285mm x 70MM x 250mm 1 84 kg DIM
21. tricci n de ciertas Sustancias Peligrosas en aparatos el ctricos electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 Directiva mec nica Machinery directive Directive m canique Para su evaluaci n se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit 61010 1 2010 EMC EN61326 1 2013 Class Safety of machinery EN ISO 12100 2010 This equipment complies with the limits for class A equipment in accordance with CISPR 11 definition and is classed as a Class A digital device pursuant to CFR Title 47 part 15 of the FCC Rules and is intended to be used in an industrial environment Consequently this product can incorporate the CE marking En consecuencia este producto puede incorporar el marcado CE C En cons quence ce produit peut incorporer le marquage CE En representaci n del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canal s o a eee Eie NU General Maridd er Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 30 04 2014 Automated place
22. weilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt E Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente I lt Nota em Protecc o Ambiental Ap s a implementac o da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte 5 Todos os aparelhos el ctricos e electr nicos podem ser despejados juntamente o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e
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