Blocking solutions
Contents
1. 01 h Block e 500 nL__ Casein base blocking solution ELISA BCL BKKE 01 k Block e 500 nL__ Degarade casein base blocking solution BCL BKBE 01 b Block e 500 nL__ BSA base blocking solution BCL BKSE 01 Blocking solution Trial set 20m x4 Trial kit containning above 4 blocking ELISA solutions j 30 Related Products To enhance antigen antibody reaction under 100 protein free condition the combination of c Block and Signal Booster Neo is highly recommended Product Product nane content Price Outline BCL SBN 01 _ Signal Booster Neo 250 250 nL 20 000 _ Protein free antigen antibody reaction enhancer BCL 125 Signal Booster 250 nl set 18 000 _ Standard antigen antibody reaction enhancer MO Selection guide of proper blocking solution Below is a general description We recommend using Trial set to select the best blocking solution Western blot Skim milk which many researchers use is good for general purpose but suppress signal strength The below shows selection guide as compared with skim milk 1 Needs higher sensitivity Permits background elevation k Block c Block 2 Needs lower background Permits lower sensitivity b Block h Block 3 General use h Block b Block 4 for phosphorylated proteins k Block b Block ELISA Antigen detecting ELISA Direct method c Block b Block k Block Antibody sandwich method k Block b Block h Block Antibody detect2. Ver 1 0 Beacle Inc Blocking solutions User s Manual Beacle Inc KYOTO JAPAN Cautions 1 Research use only Do not use for medical purpose 2 Do not dilute or add other agents in Blocking solutions to get the best result 3 In some occasions deposits may occur in solutions Please dissolve the deposit before use 10 Introduction Blocking is very important to get proper results in western blot and ELISA Beacle has developed four types of ready to use blocking solutions each of which has characteristic property eFeatures 1 Ready to Use 20 Exclusive formulation for Western or ELISA 3 Higher sensitivity than skim milk for Western Products 4 Long term preservation of pre coated plate possible by using ELISA blocking solution 20 Product Lineup Blocking solution products are listed below This manual is applied to all blocking solutions Field Product Product name content Outline BCL BKCW 01 c Block w 500 n 100 Chenical blocking solution BCL BKHW 01 h Block w 500 nL_ Casein base blocking solution Western BCL BKKW 01 k Block w 500 n___ Degarade casein base blocking solution BCL BKBW 01 b Block w 500 nL__ BSA base blocking solution BCL BKSW 01 Blocking solution Trial set 20mx4 Trial kit containning above 4 blocking Western solutions BCL BKCE 01 c Block e 500m 1000 Chenical blocking solution BCL BKHE
3. ing ELISA Direct method detection by secondary antibody k Block b Block k Block Antigen sandwich method k Block b Block h Block 5O General instruction to use Blocking solution in Western blot 1 After SDS PAGE transfer proteins to PVDF membrane 2 Soak the membrane into 10 L of Blocking solution and let it stay for 1hr while shaking 3 The rest of the process is the same as usual 60 General instruction to use Blocking solution in ELISA 1 Immobilize antibody or antigen onto each wells of microplate by usual method and wash wells by PBS T 3 times 2 Pour 200pL of Blocking solution into each well and let it stay for 1hr at 37 C 3 Discard Blocking solution and tap the plate on the paper towel to remove the solution completely 4 The rest of the process is the same as usual 5 For long term preservation of pre coated plate remove Blocking solution completely from microplate which has been antibody or antigen immmobilized and treated by Blocking solution Then dry up the plate at room temperature completely and pack in an air and light tight bag with a desiccant The plate can be stored for more than 6 months at 4 C 7O Contact information Beacle Inc manufacturer and distributor 10 25 Kamikazan Sakajiri Yamashina Ku Kyoto 607 8465 Japan E mailf technical support beacle com Website www beacle com 92
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