User Manual - Turner Designs
Contents
1. inches 4 1 cm Height with sealed housing 3 15 inches 8 cm Sensors Operational Temperature 2 to 50 C Depth Options 0 to1000 meters 998 2602 Rev B Page 13 B Wiring Guide Pin Out Interface Firmware Updating 1 POWER POWER 2 POWER POWER 3 RX RX 4 DIGITAL GND DIGITAL GND 5 TX TX 6 OPEN OPEN 7 OPEN DTR amp DSR 8 OPEN RTS amp CTS 9 OPEN OPEN ASCII output Cl connector mates with Molex 51021 0900 123 456 78 9 998 2602 Rev B Page 14 C Recommended Lab Practices for Cyclops Integrator Calibration The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels Note Extra care must be taken to avoid getting any of the electronics wet Only the face of the sensor should be submerged Covering the open end of the CI housing with a plastic bag and securing to the threaded section with a rubber band will help minimize the impact of accidental splashes 1 Use a non fluorescent container for your water samples Note Plastic may fluoresce and interfere with the sample s fluorescence If using a glass container place the container on a non reflective black surface Ensure that the sensor is more than 3 inches above the bottom of the container Ensure that the sensor is in the center of the container and has more than 2 inches clearance between the circumference of the sensor and the2. AL and press enter to get to the Main Menu From the Main Menu type 1 and press enter you will be prompted to put the Cyclops Integrator s sensor face in blank solution and press enter when ready Note Extra care must be taken to avoid getting any of the electronics wet Only the face of the sensor should be submerged Covering the open end of the CI housing with a plastic bag and securing to the threaded section with a rubber band will help minimize the impact of accidental splashes Enter Selection gt gt 1 Place all sensors in blank solution press lt ENTER gt when ready or lt ESC gt to go back to the Main Menu Refer to Appendix C Recommended Lab Practices for Cyclops Integrator Calibration Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial water or filtered water Ensure the optical face is free of air bubbles As the blank values are being set they will appear on the screen for each gain and sensor At the end you will be prompted to save the blank values Reading sensor 1 gain 1X please wait Press lt ESC gt to stop cancel Reading sensor 1 gain 10X please wait Press lt ESC gt to stop cancel Reading sensor 1 gain 100X please wait Press lt ESC gt to stop cancel Press lt ENTER gt to save blank values and return to the Main Menu 998 2602 Rev B Page 7 From the Main Menu type 7 and press enter to exit and view the data stre
3. Start gt All Programs gt Accessories gt Communications gt HyperTerminal 4 Establish a connection in HyperTerminal using the following port settings Port Settings Bits per second 9600 Data bits 8 Daas O Parity None Batty None Stop bits 1 step bis ff Flow control None Flow cork Click Apply and then OK 998 2602 Restore Defaults Cancel Apply Rev B Page 5 5 Once the HyperTerminal displays it is connected supply power to the Cyclops Integrator sensor control board by connecting the cable to 12V DC Power Supply P N 159 0191 2 2 GUI Controls After supplying power to the Cyclops Integrator sensor control board hold a white sheet of paper in front of the sensor face to determine if the sensor is functioning Application Light Source Color In Vivo Chlorophyll Blue Turbidity Infra Red No Color Phycocyanin Yellow Orange Phycoerythrin Green Fluorescein Blue Rhodamine Green CDOM FDOM Ultra Violet No Color Refined Oils and Fuels Deep Ultra Violet No Color Crude Oil Ultra Violet No Color Optical Brighteners Ultra Violet No Color PTSA Ultra Violet No Color TAN Do not look directly at the optics Ultraviolet light can be damaging to the eyes You should see a blue light emitted from the paper if checking a UV sensor There will be no observable light emitting from the Turbidity sensor Data will stream a few seconds after po
4. TURNER N DESIGNS Cyclops Integrator User s Manual August 7 2015 P N 998 2602 Revision B TURNER DESIGNS 845 W Maude Avenue Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 Table of Contents 1 Introduction 1 1 Description 1 2 Sensors 1 3 Accessories 1 4 Engineering Units 1 5 Data Stream Setup and GUI Controls 2 1 Functional Test Setup 2 2 GUI Controls Calibration 3 1 Raw Fluorescence Mode 3 2 Raw Fluorescence Blank Subtracted Mode 3 3 Direct Concentration Mode 3 4 Reporting Calibration Values 3 5 Clearing All Calibrations Integration Maintenance and Warranty 5 1 Maintenance 5 1 1 Rinsing 5 1 2 Optics 5 2 Warranty Terms 5 3 Warranty Service 5 4 Out of Warranty Service Appendix A Specifications B Wiring Guide C Recommended Lab Practices for Cyclops Integrator Calibration D Temperature Compensation Linear Range and Quenching RA AKRHRA oo WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT WEEE DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well being of our environment Accordingly we are concerned with preserving the surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products WEEE Return Process To arrange the return of an end of life product proceed as follows If you purchas
5. am Data will now be displayed as blank corrected relative fluorescence units 3 3 Direct Concentration Mode Values reported in the Direct Concentration Mode are blank subtracted and scaled to a standard solution that has a predetermined concentration estimate The steps below will ensure values are reported as Blank Subtracted Concentrations See Appendix C for Recommended Lab Practices When data is streaming type CAL and press enter to get to the Main Menu Following the same initial step as in the previous section for RFUB mode from the Main Menu type 1 and press enter you will be prompted to put the Cyclops Integrator s sensor face in blank solution and press enter when ready Note Extra care must be taken to avoid getting any of the electronics wet Only the face of the sensor should be submerged Covering the open end of the CI housing with a plastic bag and securing to the threaded section with a rubber band will help minimize the impact of accidental splashes Enter Selection gt gt 1 Place all sensors in blank solution press lt ENTER gt when ready or lt ESC gt to go back to the Main Menu Once all the blank values are set you will be prompted to save the blank values and return to the Main Menu Next from the Main Menu enter the number desired to go to a specific sensor s calibration menu For example select 2 from the Main Menu to Calibrate Sensor 1 using a 100ppb liquid standard Set Bla
6. ed your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at support turnerdesigns com Turner Designs will provide a WEEE RMA Number a Shipping Account Number and a Ship to Address Package and ship the product back to Turner Designs The product will be dealt with per Turner Designs end of life recycling program in an environmentally friendly way 998 2602 Rev B Page 3 1 Introduction 1 1 Description The Cyclops Integrator provides a package optimized for integrating Cyclops sensors into vehicles One two or three optical sensors ranging from deep ultraviolet to infrared can be mounted with the Cyclops Integrator electronics in a very compact footprint Submersible to 1000m the Cyclops Integrator is available with or without a watertight housing 1 2 Sensors The sensor s configured with the Cyclops Integrator are in situ sensors that detect relative fluorescence of specific fluorophores in water They use solid state optoelectronics an LED based light source a photodiode for detection of fluorescence and interference filters for maximizing or selecting fluorophores of interest The sensors have low power consumption and superior s
7. er capable of capturing ASCII data The Cyclops Integrator is not capable of storing data in memory it is only capable of streaming data out to a third party data logger Note Any data that are not captured by third party data loggers cannot be retrieved 998 2602 Rev B Page 4 2 Setup and GUI Controls 2 1 Functional Test Setup The Cyclops Integrator with a watertight housing has an 8 pin bulkhead connector You will use Continuous Data Cable P N 2200 160 and 12V DC Power Supply P N 159 0191 For Cyclops Integrator without a watertight housing you will use Interface Cable P N 2600 160 and 12V DC Power Supply P N 159 0191 Follow the steps below to connect the Cyclops Integrator to your computer for functional testing and calibration 1 For Cyclops Integrator without a watertight housing connect Interface Cable P N 2600 160 to the Cyclops Integrator sensor control board connector as shown below For Cyclops Integrator with a watertight housing you will connect Continuous Data Cable P N 2200 160 to the 8 pin bulkhead connector 2 Connect the cable s serial plug to your computer s serial port or USB using a Serial to USB cable adaptor if a serial port is not available NOTE HyperTerminal is not included in Windows 7 or later operating system If HyperTerminal is not available you can contact Support TurnerDesigns com or use another similar terminal program 3 Ona MicroSoft Windows computer open the HyperTerminal program
8. ignal stability Sensors are factory installed in the Cyclops Integrator package and cannot be changed or reconfigured for different applications once the package is assembled and received by the customer 1 3 Accessories Anti fouling Copper Tape 5 sets P N 2600 506 Solid Secondary Standard Cap P N 2600 900 Solid Secondary Standard SSS for in vivo Chlorophyll Phycocyanin Phycoerythrin Rhodamine and Fluorescein P N 2600 901 See Accessory Instructions on the USB Flash Drive for more information Solid Secondary Standard SSS for UV Sensors CDOM FDOM Optical Brighteners Tryptophan Refined Fuels and Crude Oil P N 2600 902 See Accessory Instructions on the USB Flash Drive for more information Note Solid Secondary Standard not available for turbidity 1 4 Engineering Units The engineering units reported by the installed sensors are Relative Fluorescence Units RFU The RFU signal is proportional to the amount of fluorescence detected by the sensor In other words high RFU values represent a high amount of fluorescence detected and low RFU values mean there is a low amount of fluorescence detected Customers have the option of calibrating their sensors using known standards for the purpose of converting RFU to actual concentration estimates 1 5 Data Stream A few seconds after power is supplied to the Cyclops Integrator data will stream at a rate of once per second The data stream can be captured by any data logg
9. inside surface of the beaker CAUTION Cyclops Do NOT get the Integrator electronics wet Lab stand with adjustable ring gt 2 inches all round Glass Beaker gt 3 inches No Air Bubbles On Optical Surface Dark Black Non Reflective Surface Ensure the optical face of the sensor is fully submerged in solution Check that the optical face is free from air bubbles To obtain consistency between measurements place sensor at exactly the same height for each sample This is easily done using a lab stand with an adjustable ring secured to the sensor cap 998 2602 Rev B Page 15 D Temperature Compensation Linear Range and Quenching Temperature Compensation Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature The following are Temperature Coefficients for common fluorescent applications Application Temperature Coefficients Chlorophyll in vivo 1 4 C Linear Fluorescein Dye 0 0036 C Exponential Rhodamine Dye 0 026 C Exponential PTSA 0 00126 C Exponential Linear Range and Quenching The linear range is the concentration range in which the fluorometer s output is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and span
10. nk for All Sensors Calibrate Sensor 1 Calibrate Sensor 2 Calibrate Sensor 3 Report Calibration Values Clear All Calibrations Exit Program From the Sensor Menu type 2 and press enter you will be prompted to enter the Calibration Value Type in the calibration standard s concentration value Then read the calibration standard solution using the Cyclops Integrator by placing the Cyclops Integrator s sensor face in the calibration solution and pressing enter Press enter to save the calibration 998 2602 Rev B Page 8 1 Set Blank for Sensor 1 if not already set 2 Set Calibration Value for Sensor 1 3 Clear Blank and Calibration Values for Sensor 1 4 Return to Main Menu Enter Selection gt gt 2 Enter Calibration Value then press lt ENTER gt 100 Place the Cl sensor face in the calibration solution press lt ENTER gt when ready Reading sensor 1 gain 1X please wait Press lt ESC gt to stop cancel Reading sensor 1 gain 10X please wait Press lt ESC gt to stop cancel Reading sensor 1 gain 100X please wait Press lt ESC gt to stop cancel Press lt ENTER gt to save calibration values and return to the Main Menu You can repeat these steps for the other sensors From the Main Menu type 7 and press enter to exit and view the data stream Data from the calibrated sensors are now reported as concentration estimates 3 4 Reporting Calibration Values Type CAL and press ente
11. on and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 5 4 Out of Warranty Service Follow steps for Warranty Service as listed above If Technical Support can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2602 Rev B Page 12 A Specifications Cyclops Integrator Sensor Control Board Minimum Power Supply 12 30V 3W Maximum Current Draw with 3 sensors Operational 80mA Data Output RS232 ASCII Sample Interval 1 second Baud Rate 9600 Diameter lt 3 inches 7 7 cm Height without sealed housing 1 625
12. r to get to the Main Menu From the Main Menu type 5 and press enter the Calibration Values for all the sensors will be shown Enter Selection gt gt 5 Sensor 1 Blanks x100 1163 RFU x10 133 RFU x1 169 RFU Calibration Value 0 00 1167 RFU Sensor 2 Blanks x100 0 RFU x10 0 RFU x1 0 RFU Calibration Value 0 00 0 RFU Sensor 3 Blanks x100 0 RFU x10 444 RFU x1 451 RFU Calibration Value 100 00 35892 RFU Press lt ENTER gt to return to the Main Menu 3 5 Clearing all Calibration Values Type CAL and press enter to get to the Main Menu From the Main Menu type 6 and press enter you will be prompted if you want to clear the calibration values for all the sensors Enter Selection gt gt 6 This will clear the calibration values for all Sensors are you sure Y N y 998 2602 Rev B Page 9 4 Integration Once the Cyclops Integrator sensors have been tested and or calibrated you can proceed with integration into your third party platform Refer to the power requirements in Attachment A to make sure that your system is set up correctly Refer to the Wiring Guide in Attachment B for the correct wiring configuration Three seconds after power is supplied to the instrument data will stream at once per second The Cyclops Integrator is available in configurations with and without a watertight housing Bevels are provided on the face of the instrument to match the exterior contours of a c
13. s to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is known as signal quenching Linearity can be checked by diluting a sample 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Fluorometer Response Curve D Sample Quenching Region Graph showing Linear and y Sample Quenching Regions of the _ Linear Region sample s response Sample Conce 998 2602 Rev B Page 16
14. tegrator and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for incidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Damage incurred in shipping is not covered 998 2602 Rev B Page 11 5 3 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call Turner Designs Technical Support and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com Carry out any adjustments or tests as suggested by Technical Support If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write the RMA number on the outside of the shipping cart
15. wer is supplied To stop data streaming and access GUI controls type cal and press enter on the Hyperterminal screen while data are streaming The Main Menu should display 7 options numbered 1 7 998 2602 1 Set Blank for All Sensors 2 Calibrate Sensor 1 3 Calibrate Sensor 2 4 Calibrate Sensor 3 5 Report Calibration Values 6 Clear All Calibrations 7 Exit Program Rev B Page 6 3 Calibration Cyclops Integrator sensors are in situ instruments that read relative fluorescence of fluorophores in water and report qualitative data Relative Fluorescence Units can be converted to actual concentration estimates using standards of known concentrations However the qualitative nature of these measurements allows for inaccuracies in the estimation of concentrations even for calibrated units Note If no calibration is performed data output from the Cyclops Integrator are reported as RFU 3 1 Uncalibrated Raw Fluorescence Mode Values in the Raw Fluorescence Mode are referred to as Raw or Relative Fluorescence Units RFU It is important to note that these values are not blank subtracted or scaled to a standard they are relative values 3 2 Raw Fluorescence Blank Subtracted Mode Values in the Raw Fluorescence Blank Subtracted Mode are noted as RFUB It is important to note that these values are blank subtracted but are not scaled to a standard they are relative values When data is streaming type C
16. ylindrical vessel If no watertight housing is supplied the customer is responsible for supplying a housing that is watertight and for using the Cyclops sensors at a depth and pressure that is rated appropriately for the sensors installed no more than 1000 meters 998 2602 Rev B Page 10 5 Maintenance and Warranty 5 1 Maintenance 5 1 1 Rinsing The Cyclops Integrator optical face should be rinsed or soaked in fresh water following each deployment ideally until it is completely clean again If an open instrument is to be stored please keep in a warm dry location to avoid moisture accumulation around the optics Excess moisture in the air may condense on the optics when deployed in cool water which could affect the data Note Extra care must be taken to avoid getting any of the electronics wet Only the face of the sensor should be submerged Covering the open end of the Cyclops Integrator housing with a plastic bag and securing to the threaded section with a rubber band will help minimize the impact of accidental splashes 5 1 2 Care for the optics The optical window should be visually inspected after each deployment following a soaking in fresh water If cleaning is needed use optical tissue to clean the window with soapy water or ethanol isopropanol Note The Cyclops should NOT come in contact with any organic solvents i e acetone or strong acids and bases 5 2 Warranty Terms Turner Designs warrants the Cyclops In
Download Pdf Manuals
Related Search