SE1200 User Manual – English
Contents
1. manual Hoefer SE1200 Easy Breeze Air gel drying system um SE1200 IM Rev E0 08 12 A tio e fe r Page finder Important Information ii Waste Electrical and Electronic Equipment WEEE se eee eee vill 1 Air Dryer Function and Description 1 lte TEE 2 Specification tere d l PA 3 2 Operating Instructons eee 4 Setting up the Dryer eee eee ee 4 Preparing the Gel for Drying ee 5 Assembling the Gel Drying Stack 7 Drying the Gels uwaa AA AAAA 10 3 Care and Maintenance ee 11 4 TroubleshootIN E ciar W A EE 13 5 Visualization Notes susa aa AE vtr 15 6 Bib OSTA PAY zwa a Ziza zw Ass amanaausa otan 16 7 Ordering Information 17 Important Information English If this eguipment is used in a manner not speci fied by Hoefer Inc the protection provided by the eguipment may be impaired This instrument is designed for indoor laboratory use only Only accessories and parts approved or supplied by Hoefer Inc may be used for operating main taining and servicing this product Warning Because this instrument can develop sufficient voltage and current to produce a lethal shock care must be exercised in its operation This instrument is designed in accordance with the EN61010 1 2001 electrical safety standard Nevertheless it should be used only by properly trained operators Read this entire manual before using the instrument and use only according to the instruction2. oper tor st cel toto ru n p ed pou it m n stroje a pou it pouze v souladu s pokyny P stroj mus b t v dy pou vaj se na v konu zemi v st ra spr vn zemn ny k zemi na s ti v stce Vyu it pouze nepo kozen elektrick dr ty a vybaven pro nap t budete pou vat V echna za zen spojen s vysok m nap t m by m la b t v souladu s EN61010 1 2001 Si ponech n stroje jako such a ist jako mo n Ot ete pravideln s a m kk vlhk m had kem Nech je n stroj nenastaven pln p ed pou it m Nejsou provozov na na n stroj v extr mn vlhkost nad 80 P ede lo kondenzaci o pron jmu jednotky na okoln teplotu nech p i p ij m n n stroj z chladn j do tepl ho prost ed Pro umo n n dostate n chlazen zajistit aby otvory n stroje jsou nevztahuje Vigtig Information Danish Hvis dette udstyr bruges i en m de ikke specifice ret ved Hoefer Inc den beskyttelse som er blevet forsynet af udstyret kan m ske svaekkes Dette instrument er designet for indend rs labo ratoriumbrug bare Bare tilbeh r og del godkendede eller forsynede ved Hoefer Inc kan m ske bruges for drive funk tionsfejl og betjening dette produkt Advare Fordi dette instrument kan udvikle tilstr kkelig sp nding og str m at fremstille et d dbringende chok skal pleje bruges i dets drift Dette instrument er designet i
3. m ssen Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des Herstellers auf um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come rifiuti municipali indifferenziati e devono invece essere raccolti separatamente Per informazioni relative alle modalit di smantellamento delle apparecchiature fuori uso contattare un rappresentante autorizzato del fabbricante Este s mbolo indica gue el eguipo el ctrico y electr nico no debe tirarse con los desechos dom sticos y debe tratarse por separado Contacte con el representante local del fabricante para obtener m s informaci n sobre la forma de desechar el eguipo Denna symbol anger att elektriska och elektroniska utrustningar inte far avyttras som osorterat hushallsavfall och m ste samlas in separat Var god kontakta en auktoriserad tillverkarrepresentant f r information ang ende avyttring av utrustningen 1 Air Dryer Function and Description The Hoefer SE1200 Easy Breeze Air Gel Dryer dries polyacrylamide gels by circulating air around gels that are supported on at least one side by semi permeable cellophane The dryer circulates air that is either at ambient tempera ture or heated to 43 C low setting or 54 C high setting Two 20 cm x 20 cm drying frames and a loading platform 20 cm x 20 cm are included The dry
4. overensstem melse med EN61010 1 2001 elektrisk sikkerhed standard Alligevel skulle det bruges bare af passende tr nede operat rer L s denne hel O pii h ndbog for bruging instrumentet og brug bare i henhold til instruktionerne Instrumentet skal altid bruges med jordblyet af netledningen rigtigt jordede til jord pa hovedled ningsudlobet Bruger bare uskadt elektrisk tr d og udstyr som v re specifik for sp ndingerne du vil bruge Alt udstyr forbundet til hoj spaending skulle vere i overensstemmelse med EN61010 1 2001 Beholder instrumentet s tor og ren som mulig T r regul rt med et bl dt fugtigt stof Lad instrumentt rken komplet f r brug Driver ikke instrumentet i yderst fugtighed oven for 80 Undga kondensation ved lade enheden equilibrate til omgivende temperatur ved tageen instrumentets fra et koldere til et varmere milj At tillade tilstr kkelig afk ling forsikrer at lufthullerne af instrumentet er ikke d kket Belangrijke Informatie Dutch Indien deze uitrusting in een manier wordt gebruikt die niet door Hoefer is gespecificeerd Nv de bescherming die door de uitrusting is verzorgd kan worden geschaad Dit instrument is voor binnenlaboratoriumge bruik enkel ontworpen Enkel onderdelen en delen keurden goed of leverden door Hoefer Nv kan voor het bedienen worden gebruikt handhavend en onderhouden van dit product Waarschuwend Omdat dit instrument voldoende spanning en str
5. tion en laissant l equilibrate d unit la temp ra ture ambiante en prenant l instrument d un plus froid un environnement plus chaud Permettre le refroidissement suffisant garantir que les conduits de l instrument ne sont pas couverts Wichtige Informationen German Wenn diese Ausr stung gewisserma en nicht angegeben durch Hoefer Inc verwendet wird kann der durch die Ausr stung zur Verf gung gestellte Schutz verschlechtert werden Dieses Instrument wird f r den Innenlaborge brauch nur daftir entworfen Nur Zus tze und Teile genehmigten oder liefer ten durch Hoefer Inc kann f r das Funktionieren das Aufrechterhalten und die Wartung dieses Produktes verwendet werden Die Warnung Weil dieses Instrument gen gend Stromspannung und Strom entwickeln kann um einen t dlichen StoB zu erzeugen muss Sorge in seiner Operation ausge bt werden Dieses Instrument wird in bereinstimmung mit dem EN61010 1 2001 elektrischen Sicherhe itsstandard daf r entworfen Dennoch sollte es nur von richtig erzogenen Maschinenbedienern verwendet werden Lesen Sie dieses komplette Handbuch vor dem Verwenden des Instrumentes und verwenden Sie nur gem R den Instruk tionen Das Instrument muss immer mit der Erdleitung der Macht Schnur richtig niedergelegt zur Erde am Hauptausgang verwendet werden Nur unbesch digte elektrische Leitung und Ausr stung spezifisch f r die Stromspannun gen verwenden die S
6. ans une mani re pas sp cifi par Hoefer Inc la protection fourni par l quipement pourrait tre diminu e Cet instrument est con u pour l usage de labora toire int rieur seulement Seulement les accessoires et les parties ont approuv ou ont fourni par Hoefer Inc pourrait tre utilis pour fonctionner maintenir et entre tenir ce produit Avertissant Parce que cet instrument peut d vel opper la tension et le courant suffisants pour produire un choc mortel le soin doit tre exerc dans son op ration Cet instrument est conform ment con u a I EN61010 1 2001 norme de s curit lectrique N anmoins il devrait tre seulement utilis par les op rateurs convenablement entrain s Lire ce manuel entier avant utiliser l instrument et l usage seulement selon les instructions L instrument toujours doit tre utilis avec l avance de terre du cordon d alimentation correctement a fond a la terre a la sortie prin cipale Utiliser le fil et l quipement lectriques seule ment intacts sp cifiques pour les tensions que vous utiliserez Tout guipement connect a haute tension devrait tre conform ment a EN61010 1 2001 Garder l instrument aussi sec et propre comme possible Essuyer r gulierement avec un doux touffer du tissu Laisser I instrument s che compl tement avant l usage Ne pas fonctionner l instrument dans l extr me humidit au dessus de 80 Eviter la condensa
7. d 0 5 2 glycerol to the final water rinse Fluorography Follow the manufacturer s instructions For fluorography agents EN HANCE and sodium salicylate add 0 5 2 glycerol to the final cold water precipitation step Load the gel onto the drying frame as described on following page Assembling the Gel Drying Stack Gel frames that are delivered in the same pack age as the Easy Breeze are preassembled When you order additional SE1210 Series gel frames you must install the eight knobs that lock the inner frame in place before they can be used Working with gel frames that are not preassembled Screw the eight black knobs into the outer gel frame until they fit just short of snug See Fig 2 Continue with step 1 of the assembly instructions Working with preassembled gel frames Turn each black knob a one guarter turn to release the inner frame See Fig 3 Gently pop the inner frame out of the outer frame and continue with step 1 of the assembly instructions 0 Place the inner frame on the loading platform with the side labeled UP facing up e Immerse a sheet of porous cellophane in distilled water and soak for several minutes Place the fully hydrated sheet over the inner frame on the loading platform Pour 5 10 ml of distilled water onto the sheet Place the gel s on the cellophane Remove all bubbles by carefully pushing them toward the edge of the gel with your gloved finger I
8. e or heat any component above 55 C 3 Care and Maintenance Cleaning After each use wipe the dryer with a damp cloth Clean each frame with mild soap and water rinse thoroughly with water and distilled water and allow to air dry Never use abrasive cleansers on any part Do not expose the frame to solutions or vapors of aromatic or haloge nated hydrocarbons ketones esters alcohols over 30 or concentrated acids over 25 Changing the Gasket If the gasket on the inner frame becomes inflex ible or no longer holds the gel stack tautly remove it and install a new gasket stretching it slightly around three sides and then rolling it into place on the fourth e pll Note Fuses protect eguipment by disconnecting loads too large for the instrument s circuit design Replace fuses only with identical fuse values 115 V model one F 12A 250V 3AG 230 V model two T 6 3A 250V 5 x 20 mm o pl2 Changing Fuses Caution Set the power switch to off and unplug the power cord before replacing the fuse e Insert a small flat blade screwdriver into the slot on the fuse module depress slightly and turn it 1 4 turn counterclockwise The spring loaded module cap will loosen and you can then pull the cap fuse holder out Pull the fuse out of its holder and inspect If the fuse element is burned or broken replace the fuse with an identical type If the fuse appears to be intact check it with a mul
9. el electrophoresis In Methods in Enzymology 65 L Grossman and K Moldave eds Academic Press N Y 365 371 1980 Perng G S Rulli R D Wilson D L and Perry G W A comparison of fluorographic methods of detection of 35S labeled proteins in polyacrylamide gels Anal Biochem 173 387 392 1988 7 Ordering Information product quantity code no Easy Breeze Air Gel Dryer Includes two 20 cm x 20 cm gel frames one 20 cm x 20 cm loading platform and 50 cellophane sheets 115 V 1 SE1200 115V 230 V 1 SE1200 230V Replacement Parts 20 cm x 20 cm frames and platform Cellophane sheets 33 cm x 33 cm 50 SE1202 Mylar sheets 33 cm x 33 cm 25 SE1204 Gel drying frame 20 cm x 20 cm SE1210 Includes inner and outer frames 8 black knobs and one O ring sealing gasket Order loading platform separately O ring sealing gasket 20 cm x 20 cm frame E1212 Knobs black 10 SE1213 Gel loading platform 20 cm x 20 cm SE1214 Outer gel drying frame and 8 knobs 20 cm x 20 cm SE1215 Inner gel drying frame 20 cm x 20 cm SE1216 and one O ring sealing gasket Replacement Parts 21 cm x 26 cm frames and platform Cellophane sheets 33 cm x 38 cm 50 SE1202 L Mylar sheets 33 cm x 38 cm 25 SE1204 L Gel drying frame 21 cm x 26 cm SE1210 L Includes inner and outer frames 8 black knobs and one O ring sealing gasket Order loading platform separately O ring sealing gasket 21 cm x 26 c
10. er can hold up to six frames and when fully loaded the dryer can accommo date up to six 20 x 20 cm or twenty four 8 x 10 cm gels With the 21 cm x 26 cm acces sory frames the dryer can accommodate up to six 21 cm x 26 cm or thirty six 8 cm x 10 cm gels Fig 1 Main components of the SE1200 Easy Breeze Mains power switch Slide assembled frames into the turns on the fan racks starting at the top slot Always place the wettest assem blies in the bottom slots Heater power switch High Off 0 Unpacking Unwrap all packages carefully and compare contents with the packing list making sure all items arrived If any part is missing contact your local sales office Inspect all components for damage that may have occurred while the unit was in transit If any part appears damaged contact the carrier immediately Be sure to keep all packing material for damage claims or for repacking should it become necessary to return the unit This declaration of conformity is only valid for the instrument when it is e used in laboratory locations e used as delivered from Hoefer Inc except for alterations described in the user manual e connected to other CE labeled instruments or products recommended or approved by Hoefer Inc Specifications 115 V model Max wattage Max voltage Frequency 230 V model Max wattage Max voltage Frequency Environmental operating conditions Installation categ
11. eratura ambiental quando tomar o instrumento de um mais frio a um ambiente mais quente Permitir esfriar suficiente assegura que as aber turas do instrumento n o s o cobertas o pvi Informaci n Importante Spanish Si este equipo es utilizado en una manera no especificado por Hoefer S a la protecci n proporcionado por el equipo puede ser da ada Este instrumento es dise ado para el uso interior del laboratorio s lo S lo accesorios y partes aprobaron o suministraron por Hoefer S a puede ser utilizado para operar para mantener y para atender a este producto Advirtiendo Porque este instrumento puede desarrollar voltaje y corriente suficientes para producir un golpe mortal el cuidado debe ser ejercitado en su operaci n Este instrumento es dise ado de acuerdo con el EN61010 1 2001 est ndar el ctrico de segu ridad No obstante debe ser utilizado s lo por operarios adecuadamente capacitados Lea este manual entero antes de utilizar el instrumento y el uso s lo seg n las instrucciones El instrumento siempre debe ser utilizado con el plomo de la tierra del cable de alimentaci n moli correctamente a la tierra en la salida de red Utiliza alambre y equipo el ctricos s lo ilesos espec ficos para los voltajes que usted utilizar Todo equipo conectado al voltaje alto debe ser de acuerdo con EN61010 1 2001 Mantiene el instrumento tan seco y limpio como posible Enjugue regularmen
12. ie verwenden werden Die ganze mit der Hochspannung verbundene Ausr stung sollte in bereinstimmung mit EN61010 1 2001 sein Das Instrument ebenso trocken halten und reini gen wie m glich Wischen Sie regelm ig mit einem weichen befeuchten Sie Stoff Lassen Sie das Instrument trocken v llig vor dem Gebrauch o piv Das Instrument in der u ersten Feuchtig keit Uber 80 nicht bedienen Vermeiden Sie Kondensation die Einheit eguilibrate zur Umgebungstemperatur laBend wenn Sie das Instrument von einem k lteren bis eine w rmere Umgebung nehmen Um das gen gend Abk hlen zu erlauben stellen Sie sicher dass die Offnungen des Instrumentes nicht bedeckt werden Informazioni Importanti Italiano Se quest apparecchiatura usata in un modo specificato da Hoefer Inc la protezione fornito dall apparecchiatura potrebbe essere indebolita Questo strumento disegnato per l uso di labo ratorio interno solo Solo gli accessori e le parti hanno approvato o hanno fornito da Hoefer Inc potrebbe essere usato per operare per mantenere e per revision are questo prodotto Avvertendo Perch questo strumento pu sviluppare il voltaggio sufficiente e la corrente di produrre una scossa letale la cura deve essere esercitata nella sua operazione Questo stru mento disegnato conformemente all EN61010 1 2001 la norma di sicurezza elettrica Tuttavia dovrebbe essere usato degli operatori solo cor
13. ion should consist of 7 acetic acid 5 methanol and 0 5 2 glycerol Glycerol maintains the flexibility of the gel making it less likely to crack Equilibrate the gel in this solution for a minimum of 2 hours but preferably overnight After destaining rinse the gel with distilled water for 1 2 minutes to remove excess acetic acid Silver stain Add 0 5 2 glycerol to the final water rinse Fluorography Follow the manufacturer s instructions For fluorography agents ENSHANCE and sodium salicylate add 0 5 2 glycerol to the final cold water precipitation step Load the gel onto the drying frame as described on page 9 Note Unless gel cracking is a recurring problem prevent gels from becoming tacky by using 2 glycerol or less in the gel soaking solution Note If you soak gels in a solution of ethanol and glycerol you can dry the gels after a 2 hour soak Thick Gels Gel thickness gt 0 75 mm and acrylamide gt 10 T Fix stain and destain the gel according to your preferred protocol Use a final destaining solution of 7 acetic acid and 5 methanol e Final soak solution Coomassie Blue After the final destain soak the gel in a solution of 35 ethanol and 0 5 2 glycerol for 1 2 hours Ethanol dehydrates the gel which mini mizes cracking during drying Do not place the gel in the ethanol solution longer than 2 hours as ethanol will begin destaining the gel Silver stain Ad
14. m frame SE1212 L Knobs black 10 SE1213 Gel loading platform 21 cm x 26 cm SE1214 L Outer gel drying frame and 8 knobs 21 cm x 26 cm SE1215 L Inner gel drying frame 21 cm x 26 cm and one O ring sealing gasket SE1216 L Fuses 115 V model F 12A 250 V 3 AC PSF12A FB 3AG 230 V model T 6 3A 250 V 5 x 20 mm PSF6 3A SB 5X20 e p17 Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support hoeferinc com Web www hoeferinc com Hoefer is a registered trademark of Hoefer Inc Autofluor is a trademark of National Diagnostics Fluoro Hance is a trademark of Research Products International Corp ENSHANCE is a trademark of Perkin Elmer GelBond is a regis tered trademark of Cambrex Corp 2012 Hoefer Inc All rights reserved Printed in the USA Hoefer
15. mportant Remove all bubbles trapped under the top sheet by displacing them with distilled water and pushing them to the edge of the gel with your gloved finger Work from the center of a single gel or if several gels are on the platform work from the center of the frame toward the edge Fig 3 Lock the frame flip the assembly over and turn the knobs perpendicular to the frames o Pour 5 10 ml of distilled water around the edges of and onto the gel Cover the gel with a fully hydrated sheet of either cellophane or mylar Cellophane Use for fluorography thick gels or to preserve a gel Fully hydrate a sheet of porous cello phane in distilled water and place it over the gel Cellophane does not guench the signal of fluor treated gels Mylar Use for contact autoradiography for gels lt 1 mm Fully hydrate a sheet of Mylar in distilled water and place it over the gel Once the gel is dried the Mylar peels off Place the uncovered side against the X ray film Position the outer frame with the Hoefer label facing up and the locking knobs facing down over the cellophane sheets and around the inner frame Press down to engage the two frames Applying pressure to opposite corners of the frame usually causes the frames to slip easily into place Turn the frame assembly over Turn each knob 1 4 turn so that the long edge is perpendicular to both frames See Fig 3 This locks the two frames toge
16. ning og str m til produsere et d delig sjokk m bli vd bekymring i dets drift Dette instrumentet er utformet i samsvar med EN61010 1 2001 elektrisk sikkerhetsstandard Likevel burde bli brukt det bare av skikkelig utdannede operat rer Les denne hele h ndbo ken f r bruking instrumentet og bruken bare gi til instruksjonene Instrumentet m alltid bli brukt med jorden blyet av kraftkabelen som riktig ha blitt jordet til jord p hovedledningen utl p Bruker bare uskadd elektrisk ledningsfremf ring og utstyr som er spesifikk for spenningene du vil bruke All utstyr koplet til h yspenning burde v re i samsvar med EN61010 1 2001 Beholder instrumentet som t rker og rengjor som mulig Visk regul rt med et mykt fuktig stoff La instrumentet t rker komplett f r bruk Driver instrumentet i ekstrem fuktighet ikke ovenfor 80 Unng kondensasjon ved la enheten equilibrate til omgivelsestemperatur ved taen instrumentets fra et kaldere til et varmere milj Til tillate tilstrekkelig kj lig sikrer at ventilasjon s pningene av instrumentet er ikke dekket o pv Wazne Informacje Polish Jezeli ten sprzet jest wykorzystywany w spos b nie okreslone przez Hoefer Inc do ochrony przewidzianej przez urz dzenie mo e zosta obni ony Instrument ten jest przeznaczony do u ytku w laboratoriach kryty tylko Tylko akcesori w i cz ci zatwierdzone lub dostarczone przez Hoefer Inc m
17. og by wykorzystane do eksploatacji utrzymania i obs ugi tego produktu Uwaga Poniewa ten akt prawny mo e by rozwini cie odpowiednich napi cie i bie cych do wyprodukowania miertelnego szoku opiek musi by wykonywane w dzia aniu Ten instrument zosta zaprojektowany zgodnie z tym EN61010 1 2001 Bezpiecze stwo elektryc zne standard Niemniej jednak nale y stosowa jedynie przez odpowiednio przeszkoleni opera tor w Znajd pa stwo to ca y podr cznika przed zastosowaniem instrumentu i stosowa jedynie zgodnie z instrukcjami Instrument musi zawsze by wykorzystane z ziemi doprowadzi do zasilania detonuj cego w a ciwie uzasadnione na ziemi w sieci wodoci gowej rynku zbytu Wykorzystanie tylko nieuszkodzona elektryc znych drut w i urz dzenia specjalne do napi zap ac wykorzystania Wszystkie urz dzenia pod czone do wysokiego napi cia powinny by zgodne z EN61010 1 2001 Kontrolowa instrumentu jako suche i czyste jak to mo liwe Wytrze regularnie przy pomocy mi kkiego wilgotnej szmatki Niech si instru mentem ca kowicie wysuszy przed u yciem Nie prowadz do instrumentu w skrajnych wilgotno ci powy ej 80 Zapobiec konden sacji najmu przez jednostk r wnowa y do temperatury pokojowej przy podejmowaniu instrumentu z ch odniejsze w cieplejszych rodowiska Aby umo liwi wystarczaj ce ch odzenia zapewniaj e rozci cia of the inst
18. oom kan ontwikkelen om een dodelijke schok te produceren moet zorg in zijn operatie worden geoefend Dit instrument is in overeenstemming met de EN61010 1 2001 elektrische veiligheidsstandaard ontworpen Niettemin zou het enkel door goed getrainde bedieningslieden moeten worden gebruikt Lees dit volledige handboek voor het gebruik het instrument en gebruik enkel volgens de instructies Het instrument moet altijd met de aardeleiding van het stroomsnoer correct grondde naar aarde aan het hoofdafzetgebied worden gebruikt Gebruik enkel onbeschadigde elektrische draad en uitrustings specifiek voor de spanningen u zult gebruiken Alle uitrustingen sloten aan aan hoogspanning zou in overeenstemming met EN61010 1 2001 moeten zijn Houd het instrument zo droge en schone zoals mogelijk Bij Wis regelmatig met een zacht temper doek Verhuur het instrument droogt volledig voor het gebruik Bedien niet het instrument in extreme vochtigheid bovenstaande 80 Vermijd condensatie door het verhuren van de eenheid in evenwicht brengt naar omgevingstemperatuur wanneer nemen het instrument van een kouder naar een lievere omgeving Om toe te staan voldoende afkoelen verzeker dat de luchtopeningen van het instrument niet bedekt zijn T rke Tietoa Finnish Jos tata varusteita k ytet n tavassa ei m ritetty Hoeferille Inc suojelu ehk isty varusteille saattaa olla avuton T m v line suunnitellaan sis laborat
19. oriok yt lle vain Vain lis varusteet ja osat hyv ksyiv t tai toimitti Hoeferin oheen Inc voi k ytt k ytt miselle valvoalle ja servicing t m tuote Varoittaminen Koska t m v line voi kehitt riitt v j nnitteen ja virran tuottaa kuolettavan j rkytyksen huolta t ytyy harjoittaa toiminnos saan T m v line suunnitellaan EN61010 1 2001 s hk turvallisuusstandardin mukaisesti Silti pit isi k ytt vain ohi oikeasti koulutetut k yt t j t Lue t m kokonainen manuaalinen ennen v linett ja k ytt vain ohjeiden mukaan V linett t ytyy k ytt aina valtanuoran maaly ijyst perusti oikein maadoittaa s hk verkkoau kossa K ytt vain undamaged s hk metallilankaa ja varusteita t sm llinen j nnitteille k ytt Kaikki varusteet yhdistetty korkeaan j nnitteeseen pit isi olla EN61010 1 2001IN mukaisesti Pit v lineen yht kuiva ja puhdas kuin mahdol o piii linen Pyyhi s nn llisesti pehme ll kostealla kankaalla Anna v line kuivua t ysin ennen k ytt Ei k yt v linett extreme ilmankosteudessa 80 n yl puolella V lt tiivistymist antamalla yksik n eguilibrate ymp r iv n l mp tilaan kun ottaminen v line kylmempi l mpim mp n ymp rist n Sallia riitt v n j hdytt minen varmistaa ett v lineen ilmarei t peitet n Information Importante French Si cet 6guipement est utilis d
20. ory Pollution degree Dimensions w x d x h Product certifications 1325 W 105 125 V 57 63 Hz 1325 W 210 250 V 47 63 Hz Indoor use 4 40 C Humidity up to 80 Altitude up to 2000 m I 2 32 x 51 x 27 cm 12 5 x 20 x 10 5 in EN61010 1 UL3101 1 CSA C22 2 1010 1 CE 2 Operating Instructions Setting up the Dryer 0 Place the dryer in a well ventilated area such as under a fume hood e Plug the mains power cord into a properly grounded receptacle The mains power switch operates the fan which circulates air that is either at ambient tempera ture or heated if the heater is switched on The three heat settings are high setting 54 C o ambient temperature low setting 43 C The indicator lamp located next to the heater switch lights up when the low or high setting is chosen Preparing the Gel for Drying Listed below are recommended procedures to ensure optimum drying results for gels of differ ent thicknesses and acrylamide concentrations Thin gels 0 75 mm or less are recommended for best results This drying method is not recommended for e Agarose gels which are generally too thick e Polyacrylamide gels attached to GelBond film Thin Gels Gel thickness lt 0 75 mm or gel thickness gt 0 75 mm and acrylamide lt 10 T Fix and stain the gels according to your preferred protocol e Final soak solution Coomassie Blue The final destaining solut
21. panning skulle vara i verensst mmelse med EN61010 1 2001 H ller instrumentet d torkar och reng r som m jlig Torka regelbundet med en mjuk fuktig trasa L t instrumentet torka fullst ndigt f re anv ndningen Fungerar inte instrumentet i extrem fuktighet ver 80 Undvik kondensering vid lata enheten equilibrate till omgivande temperatur n r ta instrumentet fr n en kallare till en varmare milj Att till ta tillr cklig kyla ser till att h len av instrumentet inte t cks o pvii English Ea French R muu German R Ea Italian R Esa Spanish 14 Swedish R o pviii Waste Electrical and Electronic Eguipment WEEE This symbol indicates that the waste of electrical and electronic eguipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your eguipment Ce symbole indigue gue les d chets relatifs a guipement lectrique et lectronique ne doivent pas tre jet s comme les ordures m nag res non tri es et doivent tre collect s s par ment Contactez un repr sentant agr du fabricant pour obtenir des informations sur la mise au rebut de votre guipement Dieses Symbol kennzeichnet elektrische und elektronische Ger te die nicht mit dem gew hnlichen unsortierten Hausm ll entsorgt werden d rfen sondern separat behandelt werden
22. rettamente addestrati Leggere questo manu ale intero prima di usare lo strumento e l uso solo secondo le istruzioni Lo strumento deve essere sempre usato col piombo di terra della spina di alimentazione correttamente hanno messo a terra alla terra alla presa di corrente principale Usa il filo metallico e l apparecchiatura solo intatti elettrici specifici per i voltaggi che lei user Tutta I apparecchiatura collegata all alto voltaggio dovrebbe essere conformemente a EN61010 1 2001 Tiene lo strumento come secco e pulito come possibile Pulire regolarmente con un morbido per spegnere il panno Lasciare lo strumento asciuga completamente prima dell uso Non opera lo strumento nell umidita estrema al di sopra di 80 Evitare la condensazione lasci ando l unit equilibra alla temperatura ambiente quando portare lo strumento da un pi freddo a un ambiente pi caldo Di permettere raffreddare sufficiente assicura che gli sbocchi dello strumento non sono coperti Viktig Informasjon Norwegian Hvis dette utstyret blir brukt i en m te ikke spesi fisert ved Hoefer Inc beskyttelsen som ha blitt git av utstyret kan bli svekket Dette instrumentet er utformet for innend rs laboratoriumbruk bare Bare tilbeh r og deler godkjente eller forsynte ved Hoefer Inc kan bli brukt for drive vedlike holde og betjene dette produktet Varsler Fordi dette instrumentet kan utvikle tilstrekkelig spen
23. rument nie obj te ubezpieczeniem Informag es Importantes Portuguese Se este equipamento usado numa maneira nao especificada por Hoefer Inc que a protecc o fornecida pelo equipamento pode ser compro metida Este instrumento projectado para uso de interior de laborat rio s S acess rios e partes aprovaram ou forneceu por Hoefer Inc pode ser usada para operar manter e servicing este produto Advertindo Porque este instrumento pode desenvolver voltagem suficiente e corrente produzir um choque letal cuidado deve ser exer citado em sua operac o Este instrumento projectado de acordo com o EN61010 1 2001 condic o de seguranca el ctrica N o obstante deve ser usado s por operadores adeguadamente treinados Leia este manual inteiro antes de usar o instrumento e use s de acordo com as instrucc es O instrumento sempre deve ser usado com o chumbo de terra do cord o de poder correta mente baseou a terra nos canos sa da principais Usa fio el ctrico s intacto e equipamento espe c fico para as voltagens que voc usar Todo equipamento conectado a voltagem alta deve ser de acordo com EN61010 1 2001 Mantem o instrumento t o seco e limpo como poss vel Limpe regularmente com um pano h mido macio Deixe o instrumento secar completamente antes de uso Nao opera o instrumento em humidade extrema acima de 80 Evite condensac o deixando o equilibrate de unidade a temp
24. s The instrument must always be used with the earth lead of the power cord correctly grounded to earth at the mains outlet Use only undamaged electrical wire and eguip ment specific for the voltages you will use All eguipment connected to high voltage should be in accordance with EN61010 1 2001 Keep the instrument as dry and clean as possible Wipe regularly with a soft damp cloth Let the instrument dry completely before use Do not operate the instrument in extreme humidity above 80 Avoid condensation by letting the unit equilibrate to ambient tempera ture when taking the instrument from a colder to a warmer environment To permit sufficient cooling ensure that the vents of the instrument are not covered Dulezit Informace Czech Pokud by toto za zen je pou ito zp sobem kter nen podle Hoefer ochrana poskytovan na z klad Inc za zen m e b t naru ena Tento n stroj je ur en pro vnit n pou it v laborato i pouze Pouze p slu enstv a sti schv len nebo poskytnut ch Hoefer Inc mohou b t pou ity pro provoz dr bu a dr b tohoto v robku Pozor Proto e tento n stroj m e vyvinout dostate n nap t a proud kter m vyr b t a smrtiacej ok p e mus b t vykon v na v jeho provoz Tento n stroj je ur en v souladu s EN61010 1 2001 elektrick bezpe nostn normy P esto e by m ly b t pou ity pouze dn vy kolen
25. t to back every half hour Check the gel or stack Reduce the amount of glycerol to 0 5 to 2 Check that you are not using two sheets of Mylar At least one sheet must be cellophane Check the heater Make sure the indicator lamp lights at the low hear or high hear settings If it doesn t light or the heater does not heat the safety switch may be tripped Check that the front and back of the dryer are unob structed and wait a few minutes for the dryer to cool then resume heating Gel turns cloudy or white Gels gt 0 75 mm thick with acrylamide gt 10 T Reduce the percentage of ethanol to 30 Do not soak the gel for more than 2 hours in the ethanol glycerol dehydration solution After Coomasie Blue staining be sure to use final destain of 7 acetic acid and 5 methanol Dryer does not function e pl4 Check that the power switch is on Check that the power receptacle is properly grounded and in working condition Check the fuses and replace if necessary If the dryer overheats make sure air can flow freely by checking that both the front and back are not obstructed If the dryer overheats more than once in the same day call Hoefer Inc Note Certain fluorographic agent manufacturers specify tempera tures higher than those reached in the Hoefer Easy Breeze e g Autofluor National Diag nostics and Fluoro Hance Research Products Interna tional which require
26. te con un suave el trapo h medo Permita que el instrumento seque completamente antes de uso No opera el instrumento en la humedad extrema encima de 80 Evite condensaci n permi tiendo la unidad equilibra a la temperatura ambi ente al tomar el instrumento de un m s fr o a un ambiente m s tibio Permitir refrigeraci n suficiente asegure que las aberturas del Viktig Information Swedish om denna utrustning anv nds i ett s tt som inte har specificeras av Hoefer Inc skyddet tillhan dah ll vid utrustningen kan skadas Detta instrument formges f r inomhuslaborato rium anv ndning bara Bara medhj lpare och delar godk nde eller lever erade vid Hoefer Inc kan anv ndas f r fungera underh lla och servicing denna produkt varna D rf r att detta instrument kan utveckla tillr cklig sp nning och str m att producera en d dlig st t m ste vas omsorg i dess funktion Detta instrument formges i verensst mmelse med EN61010 1 2001 elektriska s kerheten stan darden Icke desto mindre bor det anv ndas bara av riktigt utbildade operatorer Las denna hela handbok f re anv nda instrumentet och anv nd bara enligt undervisningarna Instrumentet m ste alltid anv ndas med jorden blyet av kraften repet riktigt grounded till jorden p det huvudutloppet Anv nder bara undamaged elektrisk tr d och utrustning specifik f r sp nningarna du ska anv nda All utrustning kopplats som till h g s
27. ther preventing slippage as the gel shrinks during drying Closed Fig 2 Frame parts and gel drying stack assembly frame size 20 cm x 20 cm or 21 cm x 26 cm Outer frame Hoefer label faces up Screw 8 knobs into the threaded holes in the outer frame Orient the frame so that the screws face down Cellophane Inner frame with word UP facing up e p9 Note Gels gt 0 75 mm thick or gels with acrylamide concentra tion gt 10 T must be dried slowly use the fan with low or no heat only Note To avoid cracking gels that are already dry do not drip liquid on them Load wet gels below drier ones e plo Drying the Gels 0 Slide each frame assembly into the Easy Breeze dryer driest assembly on top knobs facing up Turn on the power switch and then set the heater temperature switch to the high low or fan only o setting e To ensure uniform drying rotate each frame 180 back to front about every half hour Gels generally dry in 1 to 3 hours depending on gel concentration thickness the temperature setting and the amount of glycerol added Once all gels are dry turn off the fan and heater switches Remove the gels from the frames Rinse frames thoroughly with water and then distilled water after each use Optional Cellophane Trim excess Mylar For contact autoradiography peel Mylar off before exposing the gel to X ray film Note Never autoclav
28. ti meter A reading of 10 or less indi cates the fuse is still usable Insert the fuse into the holder and then insert this assembly back into the unit Seat the module by inserting the screwdriver into the slot pressing gently and turning the cap 1 4 turn clockwise 230 V model Repeat steps 2 to 4 for the second fuse 4 Troubleshooting problem solution Gel or cellophane cracks All gels Handle the gel and cellophane with care Fully hydrate the cellophane with distilled water just before use and avoid stretching it during assembly If the cellophane is drying too quickly instead of hydrating it in distilled water soak it in the same glycerol solution used for the gel Always remove all air bubbles before drying Lower the heat setting Load frame assemblies into the dryer from the top down Avoid dripping onto a partially dried gel Gradient gels or gels gt 0 75 mm thick with acrylamide gt 10 T Dehydrate the gel in 35 ethanol and 2 glycerol before drying If gels still crack increase the percentage of glycerol up to 5 Note With a higher percentage of glycerol a longer drying period is required The gels may also be flex ible and feel tacky when dry Fluor treated gel Use cellophane instead of mylar Use a thinner gel lt 0 75 mm e pl3 problem solution Gel dries too slowly or not at all Gel dries unevenly Rotate each frame 180 fron
29. up to 80 C to produce an optimum signal This dryer is not recom mended for such agents 5 Visualization Notes Direct Autoradiography Prepare the gel in the usual manner and expose to film as directed with the instructions accom panying the film Exposure times especially for medium energy beta emitters such as C and S can be quite long For maximum sensitivity of these types of labels use fluorography Fluorography Fluorography intensifies a weak beta emission signal by impregnating the gel with a suitable fluor that converts beta emission from S MC and H into light Drying the gel in cellophane does not quench this signal but for H a very weak beta emitter up to 20 of the signal may be lost If using EN HANCE or sodium salicylate as fluorography agents include 0 5 to 2 glyc erol in the cold water treatment prior to drying Dehydration steps are not required for thinner lower percentage gels which are far less prone to cracking If exposed to the maximum drying temperature 54 C these gels may form longer fluor crystals resulting in higher background on X ray exposures e p15 o pl6 6 Bibliography Juang R H Chang Y D Sung H Y and Su J C Oven drying method for polyacrylamide gel slab packed in a cellophane sandwich Anal Biochem 141 348 350 1984 Laskey R A The use of intensifying screens or organic scintillators for visualizing radioac tive molecules resolved by g
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