(P&EP) Analyzer User Manual
Contents
1. 20 G9250AA Version A 1 01 Figure 1 The P amp EP MRM Database new User Interface Home Screen Control Panel Application Notes and publications You can find more information about pesticide analysis in the application notes and publications included with your Specials Factory Information CD ROM Alternatively visit http www chem agilent com and select the Literature Library under the Quick Links QuEChERS extraction procedures and ready to use kits The QuEChERS extraction procedure for pesticide residues in fruits and vegetables is being used by labs around the world For a training video references and ready to use kits for performing QUEChERS extractions go to the following Agilent web site http quechersselectiontool chem agilent com Alternatively visit http www chem agilent com and type QUEChERS into the search field Agilent GC MS MS P amp EP Analyzer User Manual 9 Introduction What s on the Specials Factory Information CD ROM Your Pesticide Analyzer comes with a CD that contains the following information specific to your Analyzer Configuration Analytical methods e The GC MS MS checkout methods 411_checkout_PCSL M 412_checkout_PCSL M 414_checkout_SV M 415_checkout_PCSL M These methods are either a pulsed cold splitless or a solvent vent MRM method with 17 analytes and is retention time locked at the factory Another GC MS MS method is included as a reference 415_checkout_HSL m2. GC MS MS Analyzer is a complete system that is factory configured and chemically tested to quickly identify a broad range of commonly analyzed pesticide and pollutant residues with ultimate sensitivity The Analyzer comes with a Multiple Reaction Monitoring MRM database p n G9250AA rev A 1 01 that includes information for over 1000 pesticides and organic pollutants The Analyzer is configured with Agilent s proprietary Capillary Flow Technology to provide rugged reliable GC column backflushing Backflushing the GC column shortens run times extends column life reduces chemical background noise provides consistent retention times and spectra and helps keep the MS ionization source clean A Multi Mode Inlet MMI provides the flexibility to inject samples in cold hot or solvent vent modes allowing operators to optimize injection volumes to maximize mass on column A split splitless Ul inlet option is available for laboratories that do not require maximal limit of detection LOD performance Option 114 Each Analyzer system is tested using a 17 compound mixture and retention times for analysis locked at the factory Four hardware configurations are available G3445B 411 Flexible Constant Pressure CP method 40 min run 30 m column x 0 5 m restrictor post column backflush 30M Column Restrictor or a splitter G3445B 415 Fast CP method 20 min run 15 m column x 0 5 m restrictor post column backflush
3. Specify acquired ion s from scan 125 286 or MRM 208 93 in the RTL process if the locking compound is Chlorpyrifos Methyl Agilent GC MS MS P amp EP Analyzer User Manual 15 Operations Running the Checkout Sample 16 At the factory your system was chemically tested by making 1 uL injections of a 100 ppb 17 compound checkout standard The Specials Factory Information CD ROM contains a copy of the exact method used during factory checkout e 411 checkout_PCSL M e 412 checkout_PCSL M e 414 checkout_SV M e 415 _checkout_PCSL M Chromatography for your specific method was retention time locked RTL at the factory At installation Agilent s service engineer will verify chromatographic performance and relock the method The Analyzer Checkout Sample contains 17 pesticides shown in Table 1 The concentration of each pesticide is 100 ppb 100 pg uL in acetone Please note that most of the pesticides used to make up this sample were gt 97 pure meaning that there are some small impurities or isomers of the major components may be present To run the checkout sample 1 Load the specified checkout method 411_checkout_PCSL M 412 _checkout_PCSL M 414 checkout_SV M 415_checkout_PCSL M 2 Make a 1 uL Injection of the 100 ppb Checkout Sample p n 5190 0494 3 Name the Data File 411_checkout_PCSL_1uL D 412_checkout_PCSL_1uL D 414_checkout_SV_1uL D 415_checkout_PCSL_1uL D 4 Save the files in D MassHunter GCMS 1 Data
4. Agilent GC MS MS P amp EP Analyzer User Manual Table 1 Pesticides contained in the 100 pg uL checkout sample p n 5190 0494 Retention Time min Operations Name CAS Opt 411 Opt 412 Opt 414 Opt 415 40 min 40 min 20 min 20 min 20 min Dichlorvos 62 73 7 5 831 6 188 4 679 4 032 2 915 Mevinphos 7786 34 7 17 595 9 070 5 610 4 845 3 803 Ethalfluralin 55283 68 6 11 283 13 590 7 139 6 383 5 654 Trifluralin 1582 09 8 11 637 13 945 7 241 6 511 5 831 Atrazine 1912 24 9 13 159 15 321 7 887 7 149 6 576 Chlorpyrifos Methyl 5598 13 0 16 593 18 110 9 143 8 524 8 297 Locking Compound Heptachlor 76 44 8 16 796 18 299 9 339 8 663 8 387 Malathion 121 75 5 18 800 19 654 9 729 9 289 9 404 Dieldrin 60 57 1 23 870 23 391 11 717 11 438 11 940 p p DDE 72 55 9 24 021 23 418 11 612 11 441 12 018 Hexazinone 51235 04 2 27 385 26 665 13 195 13 349 13 703 Propargite 2312 35 8 27 125 27 033 13 318 13 572 13 876 Mirex 2385 85 5 29 835 29 633 14 872 14 624 14 944 Fenarimol 60168 88 9 30 403 30 320 15 084 14 831 15 237 Coumaphos 56 72 4 31 667 31 979 15 853 15 410 15 871 Etofenprox Ethofenprox 80844 07 1 33 203 33 912 16 777 16 245 16 650 Deltamethrin 52918 63 5 36 001 36 506 18 117 17 582 18 048 Agilent GC MS MS P amp EP Analyzer User Manual 17 Operations Reviewing Data and Generating a Report 1 Open MassHunter Qualitative Analysis Qual 2 Select File gt Open Data File to load the data file 411_checkout_PCSL_1uL D 412_checkout_P
5. 0680 1 0 Bleed line 0 0625 in OD x 0 010 in ID x 100 cm 316SS tubing 0100 2354 1 0 Bleed tee ferrule 1 16 inch Vespel 10 pkg 0100 1329 3 7 pieces Useful tools and chemical standards Column installation tool G1099 20030 0 1 Capillary tubing cutter 4 pkg 5181 8836 0 1 Pesticide analyzer checkout sample 5190 0494 0 1 17 pesticides at 100 pg L each in acetone Sample preparation QuEChERS EN method sample pack 5982 7000 0 1 QuEChERS AOAC method sample pack 5982 7005 0 1 Additional QUEChERS extraction supplies at www agilent com chem quechers 20 Agilent GC MS MS P amp EP Analyzer User Manual Troubleshooting Operations Use existing Data files to Lock If you have acquired all calibration runs and there was a problem of picking up the integrated peak etc you can simulate the retention time locking process without rerunning the complete RTL data set 1 2 Load the method Use Qual to evaluate all data files inside the RTLOCK folder select 1 2 representative ions of the locking compound Make sure the locking compound EIC can be properly integrated In command line type acqlock 1 1 there is a space before 1 1 You will see normal RTL message box and also the message that previous RTL datafiles will be lost This command simulated the locking process and you should answer Yes It will use existing data files in the evaluation Next you should see normal locking screens complete these and the method i
6. 15M Column Restrictor splitter Agilent GC MS MS P amp EP Analyzer User Manual Introduction G3445B 412 Fast Constant Flow CF method 20 min run 15 m column x 15 m column mid column backflush Full screening CF method 40 min run 15 m column x 15 m column mid column backflush Q Q 15M Column 15M Column or a splitter G3445B 414 Selective CF method 20 min 5 m column x 15 m column mid column backflush 414 MMI 7000C 15M Column 5M Column or a splitter Notes for Analyzer option 414 The 414 option is configured with a 240 V GC oven to allow for the rapid temperature rates in the oven and inlet programs Add an additional 2 minutes hold time to extend the GC analytical run for late eluters i e Imibenconazol Cinidon ethyl Propaquizafop Temephos and Fluthiacet methyl The 2 mm dimpled liner p n 5190 2297 aids in minimizing column contamination due to non volatile matrix deposits being retained on the dimples of the liner Your Analyzer was configured and checked at the factory to ensure that your system is ready to run samples of value to your operation immediately after installation in your lab The factory test results for your Analyzer can be found on the Specials Factory Information CD DVD that shipped with your instrument Agilent GC MS MS P amp EP Analyzer User Manual 7 Introduction Where to Find More Information about Using your Pesticide Analyzer M
7. Agilent GC MS MS Pesticides and Environmental Pollutants P amp EP Analyzer User Manual pe Agilent Technologies Notices Agilent Technologies Inc 2015 No part of this manual may be reproduced in any form or by any means including electronic storage and retrieval or transla tion into a foreign language without prior agreement and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 63445 90030 Edition Second edition June 2015 Printed in USA Agilent Technologies Inc 2850 Centerville Road Wilmington DE 19808 1610 USA Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in t
8. CSL_1uL D 414_checkout_SV_1uL D 415_checkout_PCSL_1uL D 3 Select Method gt Open to load the signal to noise method 411_signal to noise M 412_signal to noise M 414_signal to noise M 415_signal to noise M 4 Select Actions gt Run the Worklist Actions 5 Open the PDF report in the Reports folder within the data file folder If all signal to noise ratios of Mirex are greater than 2500 this system is ready for running samples 6 Compare your report with the factory report for the checkout sample generated for this specific Analyzer The results from the factory are on the Specials Factory Information CD ROM Relock the method if the Chlorpyrifos Methyl peak is more than 0 015 minute different than the method s locking compound retention time of 16 593 9 143 8 524 or 8 297 minute see Table 1 on page 17 for corresponding methods and retention times See Relocking the Data Acquisition Method on page 14 18 Agilent GC MS MS P amp EP Analyzer User Manual Operations Columns and Supplies Table 2 Columns and column accessories Description Part number installed extra Siltek deactivated Fused Silica 5 m x 0 15 mm 160 7626 5 0 7m 4 3m flow restrictor 411 and 415 Analyzers Agilent J amp W HP 5ms Ultra Inert GC Column 19091S 433 UI 1 0 30 m x 0 25 mm x 0 25 um 411 Analyzer Agilent J amp W HP 5ms Ultra Inert GC Column 19091S 431 UI 1 0 15 m x 0 25 mm x 0 25 pm 414 and 415 Analyzers Agilent J a
9. RM Database DVD G9250AA The Pesticides and Environmental Pollutants MRM Database G9250AA rev A 1 01 now incorporates a new User Interface to provide a more streamlined approach to building a customizable Target List From this list the user interface then uses the information selected from the Database to develop an MRM acquisition method or MRM quantitation method This exclusive 1074 compound MRM Database is included with your Analyzer as a separate DVD The Database DVD also includes a few different documents and guides that outline the utility and operation of the Database The G9250AA Rev A 1 01 User Manual G9250A A 90000 pdf provides a tutorial of the new User Interface see Figure 1 on page 9 that includes the following functions e Building a Target Compound List e Developing an MRM Table e Exporting the MRM data to the CLA for acquisition optimization e Developing a Quant Method e Previous Functionality of the database Agilent GC MS MS P amp EP Analyzer User Manual Introduction ee Fite Home Insert Pagelayout Formulas Data Review View MASSHUNTER REP amp cut Calibri 1 AJ Em ws gt i Wrap Text Qa Copy ape B Z uU B S A ESA GE GE By merge amp cent J Format Painter Clipboard 5 Font G Alignment Agilent Technologies MRM Database Control Panel 2 3 2 os 4 5 6 Target Compound List 7 8 5 MRM Table View Methods 12 13 14 Help 15 17 Exit
10. This is a hot splitless MRM method with 17 analytes and is NOT retention time locked at the factory e The GC MS MS general pesticide analysis methods Pulsed_cold_splitless M Hot_splitless M 414 SV M 415_PCSL M or 415_HSL M If you do not have a MultiMode Inlet MMI installed you should use the hot splitless method Hot_splitless m instead of the pulsed cold splitless method for the inert split splitless inlet e The signal to noise data analysis methods 411_signal to noise M 412_signal to noise M 414_signal to noise M 415_signal to noise M Copy the Factory Methods and Test Results to the Hard Drive D MassHunter GCMS 1 Method 10 Agilent GC MS MS P amp EP Analyzer User Manual Introduction Edit the Msdchem ini file in the C GCMS msexe folder to show for example _METHFILES 411_checkout_PCSL m so this method will be loaded as you bring up the 7000 acquisition software without going through Method Resolution The qqgacqmethod xml file in each method folder has all the MRM transitions for the method Data files and reports e Checkout sample data files 411_checkout_PCSL_1uL D 412_checkout_PCSL_1uL D 414_checkout_SV_1uL D 415_checkout_PCSL_1uL D Copy the checkout data file from the Specials Factory Information CD ROM to D MassHunter GCMS 1 Data e Report QuantReport_ESTD_ResultsSummary xlsx obtained at the factory for a 1 uL injection of the 100 ppb GC MS MS Analyzer Checkout Sample p n 5190 0494 Refere
11. column a Find your ferrule type in Table 4 and note the range of degrees to tighten the internal nut b Tighten the internal nut clockwise to the lower end of your ferrule s range c Check if the ferrule is gripping the column If yes stop If not continue to tighten the internal nut in small 5 to 15 degree increments Check after each increment to see if the ferrule is gripping the column Stop as soon as gripping occurs see Figure 5 on page 28 Table 4 Degrees to tighten the internal nut for proper swaging Ferrule part number Degrees to tighten internal nut G3188 27501 G3188 27502 G3188 27503 G3188 27504 G3188 27505 G3188 27506 50 100 degrees 30 70 degrees 20 50 degrees 60 degrees 40 90 degrees 20 50 degrees Agilent GC MS MS P amp EP Analyzer User Manual 27 Appendix A UltiMetal Plus Flexible Metal Ferrules Swaging Guide Figure5 Tightening the internal nut 4 Using the 1 4 inch open ended wrench turn the internal nut an additional 15 to 20 degrees clockwise to ensure the ferrule is properly swaged onto the column see Figure 6 Figure 6 Ferrule swaged to column and internal nut 5 Using the 1 4 inch open ended wrench remove the internal nut from the swaging wrench and column 28 Agilent GC MS MS P amp EP Analyzer User Manual Appendix A UltiMetal Plus Flexible Metal Ferrules Swaging Guide 6 Using the column cutter trim the column at the small end of the ferru
12. erstood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met Contents Introduction Analyzer Configurations 6 Where to Find More Information about Using your Pesticide Analyzer 8 What s on the Specials Factory Information CD ROM 10 See Also 12 Operations Relocking the Data Acquisition Method 14 Running the Checkout Sample 16 Reviewing Data and Generating aReport 18 Columns and Supplies 19 Troubleshooting 21 Appendix A UltiMetal Plus Flexible Metal Ferrules Swaging Guide Parts Supplied 25 Tools Required 25 Preparingthe Column Ends 26 Swaging Quality Inspection 29 Agilent GC MS MS P amp EP Analyzer User Manual 3 Agilent GC MS MS P amp EP Analyzer User Manual Agilent GC MS MS Pesticides and Environmental Pollutants P amp EP Analyzer User Manual 1 Introduction Analyzer Configurations 6 Where to Find More Information about Using your Pesticide Analyzer 8 What s on the Specials Factory Information CD ROM 10 ei Agilent Technologies Introduction Analyzer Configurations 415 Identifying and quantifying dozens or hundreds of pesticides or organic pollutants in a wide range of fruit vegetable or environmental samples is a complex task The Agilent
13. es the acquisition method so if the method is locked each data file also includes the six calibration files Workaround e Backup the D masshunter gcms 1 hst ini file e Open the original hst ini file in the notepad and modify following line copymethod enabled 0 e Save the hst ini file e Method is not saved under the data files anymore If instrument is reconfigured this file is overwritten Agilent GC MS MS P amp EP Analyzer User Manual Operations The GC never comes ready or the sequence halted Make sure all pressure setpoints can be reached or are properly ignored Fix all leaks increase gas supply pressure and configure the purged ultimate union to Ignore Ready True Checking for leaks Checking air leaks at MS transfer line connection Spray the MS transfer line nut with a fluorinated aerosol duster for example Miller Stephenson MS 222N Aero Duster and look for ions 69 and 83 from 1 1 1 2 Tetrafluoroethane CAS 811 97 2 Leak free connections UltiMetal Plus Flexible Ferrules provide a leak free seal using less torque reducing column breakage and are treated with UltiMetal Plus deactivation for an inert flow path See also e Flexible Metal Ferrule Touchless Package Video Wide opening and new dial pack design allows Touchless installation as column is threaded through ferrule easily http www chem agilent com en US products services Part s Supplies Chromatography Spectrometry GC and GC MS Ul
14. he sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or sub contract Software is delivered and licensed as Commercial computer soft ware as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer software as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclosure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Govern ment users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an oper ating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully und
15. ies 13 Operations Relocking the Data Acquisition Method Relocking 14 Mass Hunter GC MS Acquisition software Rev B 07 00 SP2 or later can perform the automated Retention Time Locking RTL calibration for constant flow methods With the release of the P amp EP 3 0 the automated Retention Time Locking RTL calibration process includes both RT Locking for both constant pressure CP and constant flow CF methods The RT_locking_calculator xlsx is being supplied with the Analyzer CD DVD for informative purposes only The calculator spreadsheet does not need to be used for relocking the method and has not been used during the instrument checkout in the factory Following replacement or trimming of the GC column Agilent recommends that you relock you analyzer method to eliminate the need for time segment adjustments in your acquisition method 1 To relock a method select Relock Method from the GC MS MS data acquisition Method menu and enter the required information to start a run The target retention time for the Chlorpyrifos Methyl peak is 16 593 9 143 8 524 or 8 297 minute See Table 1 on page 17 for corresponding methods and retention times 2 Follow the steps to obtain the lock pressure flow 3 Enter the lock pressure flow from the above relocking process into your acquisition methods 4 Save the method with the new locked pressure flow Agilent GC MS MS P amp EP Analyzer User Manual Operations The use
16. le leaving approximately 0 3 mm of the column extending from the ferrule Figure 7 The minimum size of the column head extending from the ferrule is 0 1 mm and the maximum is 0 5 mm It is important to have 0 5 mm or less column head to prevent column conflict in the union Figure 7 Properly trimmed column and ferrule Swaging Quality Inspection Figure 8 shows both an incorrect and correct swaging symmetry Incorrect Correct Figure 8 Incorrect and correct swaging symmetry If your column and ferrule look like the incorrect example in Figure 8 above it is possible that your swaging wrench is defective or worn out Try swaging with a new wrench or new nut Agilent GC MS MS P amp EP Analyzer User Manual 29 Appendix A UltiMetal Plus Flexible Metal Ferrules Swaging Guide 30 Agilent GC MS MS P amp EP Analyzer User Manual ZE gt Agilent Technologies Agilent Technologies Inc Printed in USA June 2015
17. mp W HP 5ms Ultra Inert GC Column 19091S 431 UI 2 0 15 m x 0 25 mm x 0 25 um 412 Analyzer Agilent J amp W HP 5ms Ultra Inert GC Column G3903 61005 1 0 5 m x 0 25 mm x 0 25 um 414 Analyzer Column ferrule MMI graphite 10 pkg 5080 8853 1 1 pkg Column nut GC capillary 2 pkg 5181 8830 1 1 Swaging nut for MS interface G2855 20555 1 0 OR Column nut for MS interface 05988 20066 1 0 MS interface ferrule 15 graphite 85 Vespel 0 25 mm 5062 3508 1 1 pkg 10 pkg GC inlet and instrument supplies Inlet liner 4 mm Ultra Inert liner Splitless single taper wool 5190 2293 1 0 hot splitless injections Inlet liner 2 mm Ultra Inert liner Splitless single taper 5190 2297 1 0 dimpled cold splitless injections O ring inlet liner non stick 10 pkg 5188 5365 1 1 pkg Septum advanced green 50 pkg 5183 4759 1 1 pkg Agilent GC MS MS P amp EP Analyzer User Manual 19 Operations Table 2 Columns and column accessories continued Description Part number installed extra Big universal trap for helium purification RMSH 2 2 0 Universal External split vent trap includes 3 cartridges RDT 1020 0 0 Split Vent Trap PM kit 5188 6495 0 0 Backflush and capillary flow device supplies Internal nut for capillary flow device G2855 20530 2 2 plugs installed on purged ultimate union Flexible metal ferrule UltiMetal Plus 0 4 mm id for 0 1 to G3188 27501 2 1 pkg 0 25 mm ID 10 pkg 1 16 inch Brass Swagelok tee 0100
18. nce materials e Agilent Application Notes that discuss pesticide analysis e A copy of this User Manual e Corresponding technical guides and tutorials e A list of target pesticides G9250AA_pesticide_MRM_database_compound_list pdf MRM database and Excel spreadsheet e The G9250AA rev A 1 01 Pesticides and Environmental Pollutants MRM database e The Excel spreadsheet RT_locking_calculator xlsx for calculating the proper inlet flow to relock the Chlorpyrifos Methyl peak for the method of choice is still included for optional use Agilent GC MS MS P amp EP Analyzer User Manual 11 Introduction See Also Document files e MRM Database G9250AA Rev A 1 01 Help file G9250AA_Help pdf e MRM Database G9250AA Rev A 1 01 ReadMe file G9250AA_ReadMe pdf e MRM Database G9250AA Rev A 1 01 Quick Start Guide G9250 90010 pdf e MRM Database G9250AA Rev A 1 01 User Manual G9250 90000 pdf e The Pesticides and Environmental Pollutants P amp EP GC MS MS 3 0 Application Note 5991 4419EN pdf Tutorial video Adding Compounds Adding Compounds MP4 located on G2950AA MRM Database DVD 12 Agilent GC MS MS P amp EP Analyzer User Manual Agilent GC MS MS Pesticides and Environmental Pollutants P amp EP Analyzer User Manual 2 Operations Relocking the Data Acquisition Method 14 Running the Checkout Sample 16 Reviewing Data and Generating a Report 18 Columns and Supplies 19 Troubleshooting 21 SEE Agilent Technolog
19. of RT_locking_calculator xlsx is not needed with current GC MS Acquisition software but it is still supplied for informative purposes If used 1 Obtain the RTLOCK data files in Method M folder 2 Write down the retention times and pressures flows of Chlorpyrifos Methyl transition ion s 208 93 3 Record the retention time and pressure flow of data file that was just obtained For the flow calculations use Column 1 to set the RTs 4 Enter the recorded values into their respected cells in the calculator Note make sure the Desired Locking RT is accurate for the method of choice see Table 1 on page 17 5 Enter the calculated locking RT pressure flow into the Method for column 1 Note for a constant flow method the column 2 flow setting column 1 0 2 mL min Auto Retention Time Locking RTL This Analyzer s Chromatographic analysis is retention time locked at the factory The retention time of the Analyzer can be automatically locked using either a full scan method or an MRM method To lock a method select Acquire RTLock Calibration Data from the GC MS MS data acquisition Method menu The system will automatically make 5 injections with varying inlet pressures or flows to change the retention time of a locking compound If you are using an MRM method make sure the locking compound transitions are in all the time segments where the peak will show up due to the adjustment of inlet pressure or flow
20. rt number is uniquely designed to prevent inventory mix ups and to help you find the ferrule you need quickly Color variations between ferrules are a normal result of the UltiMetal coating e Two 1 4 inch open end wrenches e Column cutting tool e Swaging wrench oft Agilent Technologies 25 Appendix A UltiMetal Plus Flexible Metal Ferrules Swaging Guide Preparing the Column Ends 1 Pass the column end through the internal nut the ferrule and the swaging wrench see Figure 3 Figure 3 Thread the column through the internal nut ferrule and swaging wrench 2 Thread the internal nut into the swaging wrench with the column protruding out of the back of the wrench see Figure 4 Figure4 Thread the internal nut onto the swaging wrench 26 Agilent GC MS MS P amp EP Analyzer User Manual Appendix A UltiMetal Plus Flexible Metal Ferrules Swaging Guide 3 Begin to swage the ferrule to the column using a 1 4 inch open ended wrench The design of UltiMetal Plus Flexible Metal ferrules reduces the probability of column breakage and minimizes the damage to the thread of the corresponding fittings Unlike other ferrule designs a reliable leak free seal is best made by tightening the internal nut to a recommended number of degrees not by applying more force Applying excessive force will not provide a better seal Also compressing the flexible ferrule less minimizes damage to fittings To swage the ferrule to the
21. s locked If you want to relock the method by using an existing data file you can simulate this as well 1 2 Load the method Make sure that data file name and folder is assigned in run method screen In command line type acqlock 2 1 Before executing the command make sure that the method is loaded and data file name and data path are entered properly You will see normal relock windows and following these compete the locking Agilent GC MS MS P amp EP Analyzer User Manual 21 Operations 22 Auto RTL failed Incomplete RTL Calibration information lon signals too weak MassHunter cannot integrate them Use MassHunter Qual to examine all 5 locking data files inside the method folder Use gt 1 ppm standards or set a higher electron multiplier Gain value if the ion signals are weak The r2 value is less than 0 95 Use MassHunter Qual to examine all 5 locking data files inside the method folder Make sure the locking compound exists in all 5 runs If not make sure the locking compound transitions exist in all relevant time segments The ions entered for MassHunter identification are incorrect Use MassHunter Qual to review the RTL data files and identify the ions acquired and then execute acqlock 1 1 in GC MS MS Acquisition command line to lock This command will reprocess the 5 previously collected data files More troubleshooting Complaint files take up a lot of space Remember that every data file includ
22. tiMetal Plus Flexible Metal Ferrules Pages Packagelnstru ctions aspx e Flexible Metal Ferrule Column Connections Video http www chem agilent com en US products services Part s Supplies Chromatography Spectrometry GC and GC MS UltiMetal Plus Flexible Metal Ferrules Pages flexiferrulepr eswag aspx or http youtu be 3GkmiVvydel This video demonstrates column connections using the Agilent Ultimetal Plus Flexible Metal Ferrules Three steps are presented 1 Pre swaging 2 Column trimming and quality inspection 3 Connecting to capillary flow devices Agilent GC MS MS P amp EP Analyzer User Manual 23 Operations 24 Agilent GC MS MS P amp EP Analyzer User Manual Parts Supplied Tools Required Agilent GC MS MS Pesticides and Environmental Pollutants P amp EP Analyzer User Manual Appendix A UltiMetal Plus Flexible Metal Ferrules Swaging Guide The available UltiMetal Plus Flexible Metal ferrule packages are listed in Table 3 and Figure 2 Table 3 Available UltiMetal Plus Flexible Metal ferrule packages Item Part number Ferrule description 1 G3188 27501 0 1 0 25 mm column id 10 pk 2 G3188 27502 0 32 mm column id 10 pk 3 G3188 27503 0 45 0 53 mm column id 10 pk 4 G3188 27504 Plug 10 pk 5 G3188 27505 0 25 0 32 mm UltiMetal column id 10 pk 6 G3188 27506 0 53 mm UltiMetal column id 10 pk aaa 588 Figure2 UltiMetal Plus Flexible Metal ferrules Each UltiMetal Plus Flexible Metal ferrule pa
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