Labculture® Plus Class II Microbiological Safety Cabinets (D
Contents
1. Labculture PLus A f i my w Esco Labculture Plus tested to EN 12469 for safety and performance Model LP2 4D1 with optional support stand Class II Microbiological Safety Cabinets The Industry s Premier Energy Efficient Solution from Esco Main Features The industry s premier Green Microbiological Safety Cabinet solution HPA Health Protection Agency Porton Down UK certified to EN 12469 New EC Fan Technology provides superior airflow stability and reduces operating costs Dual fan design to enhance safety If one fan fails minimal protection is still maintained with only one fan running Ergonomically angled front improves reach and comfort Fully closable motorized sash provides an airtight seal for better safety when cabinet is inoperative overnight Frameless shatterproof sash is easier to clean offers larger unobstructed viewing area Transparent glass sides provide a comfortable work environment New energy efficient electronically ballasted instant start T5 lighting Esco next generation Sentinel microprocessor supervises all cabinet functions A large easy to read digital display and ergonomically sized touchpad controls improve user interface Unique Dynamic Chamber plenum with angled filter delivers superb airflow uniformity Negative pressure plenum surrounds contaminated positive pressure plenum no fabric bags are used Multi piece work surface removal simplif2. Exhaust 369 m h 217 cfm Airflow Volume EN 12469 lt 64 dBA Required Exhaust With Optional 3 Thimble Exhaust Collar DesU EMP lla SIG Fy Static Pressure For Optional Thimble Exhaust Collar pe la ULPA Filter Typical Efficiency Downflow Centralized eee gt 99 999 at 0 1 to 0 3 microns as per IEST RP CC001 3 USA Fluorescent Lamp Intensity gt 1000 Lux 93 foot candles 1 2 mm 0 05 18 gauge electro galvanized steel with white oven baked Main Bod el aid epoxy polyester Isocide antimicrobial powder coated finish Cabinet Construction Work Surface 1 5 mm 0 06 16 gauge stainless steel type 304 with 4B finish Side Walls UV absorbing tempered glass colorless and transparent Cabinet Power Amp 232 W 4A Electrical Outlet Amp Fuse 5A 220 240V AC 50Hz 12 Full Load Amps 9A BTU Hr 1469 Net Weight 298 kg 657 Ibs Shipping Weight 354 kg 780 Ibs Shipping Dimensions 1450 x 920 x 1720 mm Maximum W x D x H 57A OPAO Shipping Volume Maximum 2 29 m 81 cu ft Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand Microbiological Safety Cabinets Air Quality Filtration Electrical Safety Standards IEST RP CC034 1 USA IEC 61010 1 Worldwide Compliance IEST RP CC007 1 USA EN 61010 1 Europe IEST RP CC001 3 USA UL 61010 1 USA EN 1822 Europe CAN CSA C22 2 No 61010
3. Contact your local Sales Representative for specific warranty details or docu mentation requests Biological Safety Cabinets Class II Microbiological Safety Cabinets Dual Fan System Provides the maximum possible level of safety by enabling safe cabinet shut down in the event of a single fan failure 1A Under normal operation with both fans 1B If the supply fan fails 1b downflow to 1C If the exhaust fan fails 1c the supply operating 1a the supply fan creates a negative the work area is suspended The exhaust fan still fan continues to provide inflow to the cabinet pressure surrounding the contaminated positive provide inflow to the cabinet to help maintain and downflow to the work area The control pressure plenum and pushes air across the supply containment The contro panel warns of downflow panel warns of inflow failure and exhaust filters The exhaust fan boosts the air failure pressure through the exhaust filter to create better inflow and operator protection Supply and exhaust fans automatically operating at reduced speeds extend fan life Robust Cabinet Construction and Enhanced Safety Features Rear interior stainless steel work zone with industry exclusive coved
4. Biological Safety Cabinets and Laminar Flow e Laboratory Fume Hoods e Laboratory Ovens Laboratory Incubators e PCR Thermal Cyclers e Microplate Shaker Incubators e Ultraflow Freezers cco WORLD CLASS WORLDWIDE Esco Technologies Inc e 2940 Turnpike Drive Units 15 16 e Hatboro PA 19040 USA Toll Free USA and Canada 877 479 3726 Tel 215 441 9661 Fax 215 441 9660 us escoglobal com usa escoglobal com Esco Micro Pte Ltd e 21 Changi South Street 1 Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 mail escoglobal com www escoglobal com Esco Global Offices Breukelen The Netherlands Kuala Lumpur Malaysia Manama Bahrain Mumbai India Philadelphia USA Salisbury UK Shanghai China Singapore V2_3K_05 10 2010 Esco Micro Pte Ltd Specifications Subject to Change ES1329 Esco Micro Pte Ltd PT Esco Bintan Indonesia Cert No 651333 Cert No 651333 1
5. Compatibility Esco microbiological safety cabinets are Hydrogen Peroxide Vapor HPV compliant and decontam inatable cabinets tested with both BIOQUELL and STERIS patented processes HPV is a safer and more efficient alternative to conventional decontamination using formaldehyde CH 0 e HPV is non carcinogenic and odorless while formaldehyde is carcinogenic toxic and has pungent smell e f there is a gap on the cabinet sealing escaping HPV to the lab will decompose to become oxygen and water Escaping for maldehyde however is harmful to people in the lab Therefore HPV decontamination can be performed while people are work ing inside the lab while formaldehyde decontamination must be performed with no one present in the lab The HPV method improves safety productivity and reduces the time to seal the cabinet e HPV biological efficacy is independent of environmental variables whereas for maldehyde efficacy is dependent on such variables e HPV has a better penetration capacity resulting in a full decontamination of the cabinet The formaldehyde method is known to result in incomplete decontamination e HPV is more effective and rapid against microbiological organisms compared to formaldehyde e HPV requires approximately 4 7 hours for set up decontamination and tear down compared to a total of 12 15 hours needed to complete a formaldehyde decontamina tion process e HPV decontamination effectiveness is
6. The sliding window can be fully opened to insert and remove larger instrumen tation and equipment Electrical Safety and Certification All components meet or exceed applicable safety requirements Each cabinet is individually factory tested for electrical safety Documentation specific to each cabinet serial number is maintained on file Tested to EN 12469 the renowned world standard for microbiological safety cabinets Labculture PLus Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small blue arrows A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone Air returns to the common air plenum where the 33 exhaust and 67 recircu lation process is continued Contact Esco or your Sales Representative for site preparation information see Electrical Specifications Warranty The Labculture Plus is warranted for 3 years excluding consumable parts and accessories e Each cabinet is shipped with a compre hensive User Manual complete with a report documenting all test procedures Additional IQ OQ documentation is available upon request
7. air temperature and microbial species in the work zone Ergonomic Lab Chair Microscope Viewing Device IV Bar with Hooks e Laboratory grade construction meets e Mounting and viewing pouch integrated e Stainless steel construction Class 100 cleanliness alcohol resistant into sash e Available for all standard cabinets PVC materials e Factory installed specify when ordering e Adjustable 395 490 mm 15 6 19 3 Labculture PLus Biological Safety Cabinets Class Il Microbiological Safety Cabinets Microbiological Testing Esco performs testing in accordance with more than 10 of the world s most recognized standards for local regional and interna tional criteria Testing in our microbiology laboratory is conducted according to NSF49 EN12469 and JIS K3800 An NSF accredited biohazard cabinet field certifier is available in house full time to supervise all testing work using harmless Bacillus Subtilis bacteria that is used to challenge the cabinet then incubated for 48 hours and the Colony Forming Units CFU are counted to determine the testing results Increased microbiological challenge tests with objects inside the cabinet work zone Bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel Protection Test The test objective is to evaluate the safety of the cabinet for the personnel operating on potentiall
8. corners makes cleaning easy Helpful for certifiers the hinged maintenance assembly opens to a fixed position on integrated gas spring struts providing front service access 7 All key components with the exception of the motor fan assembly are mounted outside the air stream and away from contaminated air to permit service without decontamination These include fluorescent lamps UV lamps electrical harnesses electronic boards and microprocessor control Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical shock are color coded red to warn service technicians The telescoping Dynamic Chamber plenum minimizes physical lifting and accelerates filter change when required Work area containment is maintained even when removable components are lifted out for cleaning The lower drain trough is a single piece fabrication with wide open angles Comprehensive Performance Testing At Esco Every Labculture Plus model manufactured by Esco is individually tested documented by serial number and validated with the following test methods nflow downflow velocity PAO Aerosol challenge for filter integrity Light noise and vibration Airflow pattern visualization Flectrical safety to IEC61010 1 Additional KI Discus containment and microbiological testing is performed on statistical sampling basis ESO WORLD CLASS WORLDWIDE Options and Acces
9. in dependent of temperature and humidity Formaldehyde requires temperature above 20 C and relative humidity above 65 e For information on the BIOQUELL and STERIS HPV methodologies contact Esco or your Sales Representative and ask for our HPV Decontamination Whitepapers KlI Discus Containment Test According to EN 12469 2000 Operator Protection Esco is currently one of the few companies in the world equipped to perform the KI Discus test for our customers The KI Discus test is de fined in the European Standard for microbio logical safety cabinets EN12469 2000 as a test method for validating the operator personnel protection capabilities of the cabinet e The KI Discus test shows excellent correla tion with the microbiological test method for operator protection and is useful for validating the actual containment perform ance of the cabinet on site e The Kl Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Esco Labculture Plus LP2 models are factory tested on a sampling basis using the KI Discus method for operator safety ESCO C D OOL CLASS WORLDWIDE 10 Model LP2 4D1 Microbiological Safety Cabinet Technical Specifications 00 Oo e 00 DD C146 55 LN lt Pa O 638 mm 25 1 ke 1222 mm 48 1 1340 mm 52 7 84
10. typical efficiency of gt 99 99 for 0 3 micron particles e Filter assembly is constructed in accord ance with EN1822 requirements e The supply filter provides ISO Class 3 per ISO14644 1 clean air to the work surface in a gentle vertical laminar flow for product protection e Modern separatorless mini pleat filter construction maximizes the filter surface area to extend filter life and eliminates possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction e The exhaust filter traps biohazard par ticles acquired from the work surface before air is exhausted to the room offering personal and environmental protection Enlarged multi line digital read out with alpha numeric display indicates all input status and alarm functions WARNING Indicators for filter life and UV life are reverse scaled from 100 to 0 and displayed on the LCD panel SENTINEL MICROPROCESSOR CONTROL SYSTEM When programmed ON e the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restricts unauthorized adjustments e an airflow alarm warns of deviations from normal velocities Mini pleat Separatorless Filter left vs Conventional Aluminium Separator Filter right Esco cabinets use Swedish Camfil Farr mini pleat filters without aluminum separators to increase filter efficiency minimize the
11. 1 ISO 14644 1 Class 3 Worldwide AS 1386 Class 1 5 Australia JIS B9920 Class 3 Japan Type tested to EN 12469 2000 Europe ESO CD OOL CLASS WORLDWIDE escoglobal com at LIVE SUPPORT Biological Safety Products Cleanroom Products Containment Pharma Products Ductless Fume Hoods Carbon Filtration General Purpose Scientific Equipment Industrial Lab Equipment In Vitro Fertilization Products PCR Products Pharmacy Products Lab Animal Research Products Lab Thermostatics Products Lab Ventilation Chemical Fume Products Lab Furniture Powder Handling Products Since 1978 Esco has emerged as a leader in the development of controlled environment laboratory and cleanroom equipment solutions Products sold in more than 100 countries include biological safety cabinets cleanroom products compounding pharmacy equipment containment pharma products ductless fume hoods in vitro fertilization workstations lab animal research products laboratory fume hoods laboratory ovens and incubators laminar flow clean benches and PCR products and instrumentation With the most extensive product line in the industry Esco has passed more tests in more languages for more certifications throughout more countries than any biosafety cabinet manufacturer in the world Esco remains dedicated to delivering innovative solutions for the clinical life science research and industrial laboratory community www escoglobal com
12. 2 mm 33 1 1 27 Exhaust filter 5 IV bar Retrofit Kit provision 8 Fluorescent lamp 11 Motorized sash 2 1 Exhaust filter 6 Service fixture retrofit kit 9 Stainless steel armrest 12 Electrical outlet Retrofit Kit provision 3 Fan provision for gas air and vacum 10 Esco Sentinel microprocessor 13 Multi piece stainless steel work 4 Downflow filter 7 Electrical amp electronic panel control system surface Optional Exhaust Collar Positions for Thimble Ducting for Model LP2 4D1 275mm 10 8 os Elo a S N 441 mm 17 4 z E LAO N N Side View Top View Labculture PLus Biological Safety Cabinets Class Il Microbiological Safety Cabinets General Specification Labculture Plus Class II Microbiological Safety Cabinets D Series Model LP2 4D1 Nominal Size 1 2 meters 4 1340 x 790 x 1545 mm 52 8 x 31 1 x 60 8 Without Base Stand External Dimensions W x D x H With Optional Base Stand 1340 x 790 x 2256 mm 711mm 28 type 52 8 X 31 1 x 88 8 1222 x 638 x 709 mm 48 1 x25 1 x 27 9 Usable Work Area 0 6m 6 5 sq ft Internal Work Zone W x D x H Tested Opening 200 mm 7 97 Working Opening 2mm 3 Inflow 0 42 m s 83 fpm Average Airflow Velocity Downflow 0 30 m s 59 fpm Inflow 369 m h 217 cfm Downflow 766 m h 451cfm
13. chance of leakage and to prolong filter life Filters include a lightweight alumi num frame for structural stability and elimination of swelling common to conventional wood frames e The exhaust filter media is protected from mechanical damage by an inte grated metal screen guard which is absent from conventional HEPA filters Sentinel Microprocessor Control Alarm Monitoring System The Esco Sentinel microprocessor based control system supervises operation of all cabinet functions e Continuous monitoring of cabinet air flow is displayed on a bright easy to read LCD panel The large display monitors operational parameters e The control panel is located at the center of the cabinet and angled down for easy access by the operator e Two integrated temperature com pensated true airflow velocity sensors provide independent measurement of inflow and downflow velocities despite room temperature fluctuation e All electronic parts are contained inside a plug and play module that permits easy exchange If required e Sentinel functions are factory set to default ON or OFF depending on worldwide destination and local preferences Default settings can be user activated through the touchpad data entry access e Automatic start up sequence will pre pare the cabinet for normal operation and advise when safe conditions are established Typical Penetration Esco Filter Efficiency Independent supply and
14. detailed in the Operations Manual A key switch located on the cabinet prevents unauthorized use of the cabinet 7he motorized sash is controlled by an up down button Cabinet Construction Robust construction and enhanced safety features qualify the cabinet for the most demanding laboratory applications The cabinet is fully assembled and ready to install and operate when shipped e The interior and back wall are formed from a single piece of stainless steel with large radius corners to simplify in terior cleaning e The cabinet work zone has no welded joints to collect contaminants or rust e All stainless steel work surfaces are accessible for cleaning e Multi piece tray components are easily lifted and removed to encourage sur face decontamination e A recessed central area and stainless steel drain pan channels spills and prevent liquids from entering the lower filtration and fan systems Fsco Labculture Plus cabinets use dual German made ebm papst centrifugal fans with EC motors The dual fan design enables inflow and downflow velocities to be precisely balanced therefore delivering maximum product operator and environmental protection With EC technology AC from the mains is converted into DC via proprietary electronics which enables up to 90 efficiency to be reached across a very wide speed and load range or as little as 1 3 the energy of industry standard fans FC systems generate less heat
15. exhaust filters provide 99 999 typical efficiency for particle sizes of 0 1 to 0 3 microns Labculture Plus Class Series filters meet the IEST RP CCO01 3 recommended practice 0 0 05 0 10 0 15 0 20 0 25 0 30 Particle Size um Typical Penetration Labculture PLus Biological Safety Cabinets Class II Microbiological Safety Cabinets 0 40 0 50 for ULPA performance USA and EN 1822 for H14 performance EU Programmable automatic UV light timer simplifies operation enhances contamination control extends UV lamp life and saves energy Color coded indicator lamps display green for fan operation blue for fluo rescent lights and electrical outlet and orange for UV lamp ON caution ESCO CLASS Il BIOSAFETY CABINET Read all safety related instructions before use Operate unit continuously for best performance Test certify this cabinet at least annually Deika tro meas EE ADIE Sadety f Protection Staredarcls HHD 9001 Gasty Certiied Mansiertunrg Eri Enlarged touchpad data entry buttons with tactile feedback permit control settings and access to diagnostics default settings and hierarchical menus e An administrator controlled PIN Per sonal Identification Number can be set to restrict access to main menu e The airflow alarm can be activated or deactivated depending on user prefer ence and nature of the work Consult your E
16. h control is mounted on the front control panel Integrated sash proximity contacts sense proper sash position serve as an interlock for the UV lamp and activate an alarm if the sash is improperly positioned When fully lowered the sash seals automatically against a closed cell peripheral gasket to isolate the interior and prevent escape of contaminants during decontamination Containment and Protection The Esco Labculture Plus microbiological safety cabinet D Series provides opera tor product and environmental protection against Biosafety Levels 1 2 and 3 This cabinet can be used for handling Biosafety Level 4 provided that the operator wears positive pressure suit e The airflow ratio of 67 recirculation to 33 exhaust increases operator pro tection beyond the 70 30 ratio of conventional microbiological safety cabinets e Inflow of room air enters the front air grille to establish operator protection room air does not enter the work zone preventing product contamination 830 Watts 7 2A H 850 Watts 7 44 1035 Watts 9 0A Power Consumption Watts 800 1000 1200 e Raised armrest prevents the likelihood of inflow grille blocking by operator s arms e The downflow supply filter is tilted proportionally to the cabinet front angle to direct more air forward to the front air grille e The inflow velocity downflow velocity airflow path and intake geometry are precision tu
17. ies cleaning Raised armrest maintains safety by preventing blockage EN T Esco Labculture Plus Class II Microbiological Safety Cabinet Model LP2 4D1 Shown with optional telescoping stand with leveling feet Green Product Labculture PLus Biological Safety Cabinets Class Il Microbiological Safety Cabinets Dual long life ULPA per IEST RP CC001 3 filters for supply and exhaust airflow Independent exhaust sensor mounted exterior to work area Single piece coved corner back wall construction Improved lighting is brighter more uniform and reduces glare Optional UV lamp operates on programmable timer HPV compliant and approved for safe decontamination tested with both BIOQUELL and STERIS patented processes Advanced Engineering The Esco Labculture Plus microbiological safety cabinet includes a number of design and performance features not found on our popular Labculture series cabinets These include e An aerosol tight window for additional safety while the cabinet is inoperative e Dual fan design guarantees safety in the event of the failure of one fan e Motorized front sash for one hand operation e Larger LCD display for easy monitoring of operational parameters Esco LP2 232 Watts 1 24 Competitor T Product 1 400 Watts 1 7A Competitor T Product 2 Competitor B Competitor N The front sash is motorized for convenient one hand operation The sas
18. ned and tested to create an optimum air curtain at the front aperture this curtain maintains per sonnel and product protection even in the unlikely event of a severe inflow or downflow imbalance that would compromise protection in a conven tional cabinet Energy Efficiency Comparison Chart Esco Labculture Plus Cabinets offer the best energy efficiency of any microbiological safety cabinet on the world market for lowest total lifecycle costs Its dual EC Fan system operates at 232 Watts 1 2m cabinet dual exhaust filter and saves up to US 500 per year per cabinet compared with competing models Lower operating watts also reduces building heating load ESCO mmm FRET CLASS WORLDWIDE Operator Product and Environmental Protection Labculture Plus Class Il Model LP2 4D1 provides protection from airborne contaminants to the operator product and the environment Model LP2 4D1 shown with optional telescoping height support stand Integrated Filtration System Independent supply and exhaust filters provide 99 999 typical efficiency for par ticle sizes of 0 1 to 0 3 microns Labculture Plus filters meet the IEST RP CC001 3 recommended practice for ULPA per formance USA and EN 1822 for H14 performance EU e ULPA filters per IEST RP CC001 3 are tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micron par ticles these provide better filtration capability than conventional H13 HEPA filters that have a
19. sco Operating Manual or contact Esco or your Sales Representative for information on user preference pro gramming capabilities built into the Sentinel microprocessor platform Redundant Fan System The Labculture Plus fan system is designed for high performance operation redun dancy maximum energy efficiency and minimal maintenance e Dual permanently lubricated direct drive external rotor motor fans provide safety in the event of a motor failure e The external rotor motor design allows for optimum cooling of the motor during extended operations and ex tends the motor bearing life e The inflow and downflow balance is precisely established by two indepen dent fans e The EC Fan maintains constant stable airflow despite building supply voltage fluctuations e Built in RFI and electrical noise filters eliminate interference with adjacent instrumentation e An integral fan hour meter tracks operating life and aids in predictive main tenance planning e To prevent fan damage a paper catch grille traps papers or towels that may drop down on the drain pan preventing them from being pulled into the column by fan suction Green Energy Efficient EC Fans Esco Next Generation Sentinel Microprocessor Control System The Sentinel microprocessor based control and alarm system supervises all cabinet functions Setpoints and other applications are user activated through touch pad programming access
20. sories Esco offers a variety of options and accessories to meet local applications Contact Esco or your local Sales Representative for ordering information Support Stands e Fixed height available 711 mm 28 0 e Adjustable height hydraulic range e Telescoping height nominal range or 864 mm 34 0 from 737 mm 29 0 to 838 mm 33 0 from 737 mm 29 0 to 838 mm 33 0 With leveling feet 38 1 mm 1 5 Electrical lift Adjustable in 25 4 mm With casters With casters 1 0 increments Electrical Outlets and Utility Fixtures e Electrical outlet ground fault e Electrical outlet Europe Worldwide e Petcock air gas vacuum North America North America American style Europe Worldwide style DIN 12898 DIN 12919 DIN 3537 Cabinet Accessories Germicidal UV Lamps Ergonomic Foot Rest PVC Armrest e Controlled by automatic UV lamp timer e Angled helps maintain proper posture e Chemically treated improves through Sentinel microprocessor e Adjustable height operator comfort easy to clean control panel aid i E e Anti skid coating chemical resistant 711 mm 28 0 standard size e Emission of 253 7 nanometers for most finish efficient decontamination e Lamp Is positioned away from operator line of sight for safety and proper exposure to interior surfaces Note UV lamp intensity reduces over time and its effectiveness is subject to factors such as relative humidity in the cabinet ambient
21. than conventional motors for cooler working conditions and higher bearing life expectancy therefore delivering superior reliability FC systems are quieter and improve working conditions in the laboratory FC systems can be operated at higher RPM s therefore delivering greater torque than conventional fans This enables constant airflow to be delivered at higher pressure losses thus lengthening filter life and reducing maintenance costs ESCO WORLD CLASS WORLDWIDE _ ULPA filtered air C Unfiltered potentially contaminated air L Room air Inflow air Dynamic Chamber Plenum Design E Positive pressure E Negative pressure The Esco double wall design creates a Dynamic Chamber plenum which surrounds contaminated areas with negative pressure preventing the possibility of contamination from leaks in filter seal gasket or cabinet structure e External surfaces are coated with Esco Isocide antimicrobial coating to pro tect against surface contamination and inhibit bacterial growth Isocide elimi nates 99 9 of surface bacteria within 24 hours of exposure e There are no screws in the front or sides to trap contaminants or complicate cleaning Comfortable Ergonomic Design The cabinet is engineered for comfort utility value and safety e The 8 5 angled viewing window and narrow profile front grille improves reach into the work area Cabinet Filtration System Dynamic air barrier inflo
22. w and forward directed downflow air converge Ambient air is pulled through the perforations located towards the work zone front to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air plenum fan plenum at the top of the cabinet Approximately 33 of the air in the common plenum is exhausted through the ULPA filter to the room The remain ing 67 of the air is passed through the downflow ULPA filter and into the work area as a vertical laminar flow air stream bathing the work surface in clean air The uniform non turbulent air stream protects against cross contamination within and throughout the work area The instant start 5000k fluorescent lamp operates on an electronic ballast to reduce heat improve comfort and conserve energy The lamp delivers uniform lighting to the work surface for greater comfort reduced glare and improved productivity see Specifications The front armrest is raised above the work zone to improve comfort and to minimize blockage of forward airflow perforations The optional adjustable support stand provides work surface height control The frameless sash eliminates blockage of operator s line of sight A generous sash opening allows for easier access into the work zone provides ample room for transferring of small equipment see Specifications
23. y hazardous samples in the cabinet work zone e A nebulizer containing 55 mL of 5 to 8 x 108 spores mL B Subtilis spores is placed inside the work zone 10 cm 4 behind the front opening sash e Target slit air samplers and impingers are placed outside the work zone to capture possibly escaping B Subtilis spores then the sample is incubated e Acceptance The number of Bacillus Subtilis CFU recovered from the agar plates shall not exceed 10 CFU per test Product Protection Test The test objective is to determine cabinet pro tection to the product samples inside the cabinet work zone from environmental contaminants e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B Subtilis is placed at 10 cm 4 in front of sash window e Target agar plates are placed throughout the entire work surface e Acceptance The number of Bacillus Subtilis CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet pro tection from cross contamination of samples placed simultaneously inside the work zone e Anebulizer containing 55 mL of 5 to 8 x 104 spores mL is placed against one of the work zone sidewalls e Target agar plates are placed 360 mm 14 away from the same side wall e Acceptance The number of Bacillus Subtilis CFU recovered on agar plates shall not exceed 2 CFU per test HPV Test Compliant Safer Hydrogen Peroxide Decontamination
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