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Using the Manual - Artisan Technology Group

1. Flush Solenoid is Sample T Flush Line al ECN Tubing PTN Sample Jj Tubing Na a Flush Syringe 7 lt 1_ _ Sample Syringe Solvent Filter l Syringe plunger Flush Syringe Sample Syringe Plunger Slot Plunger Slot Thumbscrew Thumbscrew Figure 7 3 Dual Pump System Sample and Flush Syringes 2 Loading the New Syringes 1 Select a new sample and flush syringe See Table 7 3 for a list of available replacement sample syringes and their part numbers Thereis a single 2500 uL replacement flush syringe For the flush syringe you can order the syringe Part No 0992 3219 or the 2500 uL Syringe Startup Kit Part No N293 0213 The Startup Kit includes the syringe union and the tubing needed to accommodate the 2500 uL volume Fill the inside of each new syringe with isopropanol moving the syringe plunger up and down the entire inside surface Remove all of the isopropanol bubbles from each syringe Leave enough volume of iso propanol in each syringe so that when you thread it into the Dual Pump system there are no air bubbles in the top of the syringe For the flush syringe slide the plunger into the flush syringe plunger slot Thread the top of the flush syringe into the flush solenoid Artisan Technol
2. Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining the Active Method Samples Only Example of Sample Injection Order The following example shows the order in which the samples are injected for the previous method METHOD SCREEN METHOD0O0 CHANGED FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 10 0 ENTER FIRST VIAL NUMBER 1 225 M a ES ee ee 0 Injection Order Vial Vial Type Injection Volume Sample 10 ANAeGAAWBNH RA OMDYH A gt Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Active Method All Options Enabled Creating Methods and Sequences Defining the Active Method All Options Enabled This section shows how to change the default method so that you can add standards perform dilutions or derivatizations and set up timed events The procedures in this section assume that you have enabled all of the appropriate Method Options as defined in the Adding Method Options section of Chapter 3 in this manual The Method Options Screen with the appropriate options enabled is repeated here for your convenience The RANDOM SAMPLING and METHOD EDIT options are special proce dures described in later sections in this chapter the SERIAL DILUTION option is described in Appendix C METHOD OPTIONS SCREEN METHOD OPTIONS STANDARDIZE on TI
3. Caution Do not operate in a Cold Room or a refriger Waming This instrument is not designed for operation in an explosive environment Installation Category This instrument is able to withstand transient overvoltage according to Installation Cat egory II as defined in IEC 1010 1 Pollution Degree This equipment will operate safely in environments that contain nonconductive foreign matter up to Pollution Degree 2 in IEC 1010 1 Storage Conditions The Series 200 LC Autosampler may be stored under the following conditions ambient temperature is 20 to 60 C ambient relative humidity is 20 to 80 without condensation altitudeis in the range 0 to 12 000 meters Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Safety and Regulatory Information Temperature Humidity and Environment S 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Table of Contents Preface Using the Manual Using the Manual P 1 Definitions P 2 Method P 2 Screensand Pages P 2 Main Screen P 2 Active Method P 3 Sequence P 3 Priority Method P 3 Conventions P 4 Customer Service P 5 Chapter 1 Introduction A Description of the Autosampler 1 2 Series 200 LC Autosampler Features and Benefits 1 3 Main Components of the Series 200 LC Autosampler 1 4 Keyboard and Display 1 4 Input Output
4. rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Intoduction This chapter introduces you to the Series 200 LC Autosampler and includes the following information e A Description of the Autosampler e The Autosampler s Features and Benefits e The Major Components of the Autosampler e Using the Keyboard and Display e Instrument Specifications Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com A Description of the Autosampler Introduction A Description of the Autosampler Oni inane BELLE ag LOLA HTT ARRAN TELAT TL ARTA See HHH HHH Figure 1 1 The Series 200 LC Autosampler The PerkinElmer Series 200 LC Autosampler is an advanced sample processor that can be used with virtually every liquid chromatography system or application You configure the Autosampler and define all of your methods and operations using function keys on a key board located on the front panel of the instrument A backlit Liquid Crystal Display LCD located directly above the keyboard shows both status information about the instrument and provides access to the screens you use to define methods and set configuration parameters If you use the PerkinEImer Series 200 LC Pump you will find that the Autosampler front panel is almost identical to the one on the Pump In addition the Pump and the Autosam pl
5. Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Defining the Communications Options Accessing the Communication Screen DEFAULT MAIN SCREEN METHODOO READY E E sea oir a erty From the Default Main Screen press F7 to move to the Configure Screen CONFIGURE SCREEN CONFIGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION LOG INSTRUMENT LOG inst coumfoiac Pca Jroc __ rark ver Press F2 to move to the Communication Screen The default communication option is NONE COMMUNICATION SCREEN COMMUNICATION NONE BCD POSITIVE EXTERNAL CONTROL BCD NEGATIVE BIN POSITIVE BIN NEGATIVE MI E ee ed A E 3 18 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Communications Options Configuring the Autosampler Selecting the Communication Option COMMUNICATION SCREEN COMMUNICATION NONE BCD POSITIVE EXTERNAL CONTROL BCD NEGATIVE BIN POSITIVE BIN NEGATIVE NONE re BIN GREGS Ge Gs To select a communication option press the corresponding soft function key For example 1 to select BCD POSITIVE press F5 The available options are summarized below BCD POSITIVE Binary Coded Decimal positive polarity Range of values for output 0 99 BCD NEGATIVE Binary Coded Decimal negative polarity Range of values for output 0 99 BIN POSITIVE B
6. Press F5 to start the test The following screen appears THIS TEST WILL DELETE ALL METHODS SEQUENCE CONFIGURATION AND CALIBRATION PARAMETERS CONTINUE BBUP BATTERY BACKUP TEST KEN EE E E E E E 2 Press F1 to run the test or press F2 EN to redisplay page 1 of the Diagnostics Screen If you run the test the following screen appears TURN SERIES 200 OFF FOR AT LEAST 1 MIN PRESS F8 SOFTKEY IMMEDIATELY AFTER POWER UP BBUP BATTERY BACKUP TEST 6 12 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics Backup Battery Test continued 3 Follow the instructions on the screen Ifthe test is successful the following screen appears BBUP BATTERY BACKUP TEST EA Es ee If the test fails the following screen appears Call your PerkinElmer Service Engineer TEST PATTERN NOT DETECTED IN U305 BBUP BATTERY BACKUP TEST 6 13 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Running the Diagnostic Tests Peltier Communications Test This test is designed to test communication between the optional Peltier Accessory and the Autosampler through the Peltier Connector on the rear panel of the Autosampler See Fig ure 6 2 for the location of the connector If a failure occurs call your PerkinElmer Service
7. 3 Reinsert power input module cover back into power input module Screw Fuse Block Ba J o Nove F Voltage Selector SD Shei n e x me Card Np ME Voltage Selector Power Input a gt Arrow Module Cover CA Dual Fuses Figure 2 3 Fuse Location 2 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making the Electrical Connections Installation Dual Fuse Configuration 1 Select the required fuse from Table 2 6 below Table 2 6 Dual Fuse Configuration Table Use only 5 x 20 mm 250V Rated Fuses Voltage Selector Insert the following fuses PN 0 8 A Type TT 0999 1670 0 8 A Type TT 0999 1670 2 Loosen the screw on the fuse block and remove the fuse block from the power input mod ule cover See Figure 2 3 3 Invert the fuse block and reinstall it on the power input module cover Tighten the screw 4 Slide two fuses into the fuse block 5 Reinsert the power module cover back into the power input module 2 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making the Electrical Connections Installing the AC Line Cord Waming Ground circuit continuity is vital for safe operation of equipment Never operate equipment with grounding con ductor disconnected Do not remove or alter the ground pin on a three prong line cord The line cord should have the electrical
8. ENTER FIRST VIAL NUMBER 1 225 M Be ES Be a El ee 2 Press F4 to move to the Method Dilution Screen METHOD02 CHANGED DILUT ENTER THE NUMBER OF DILUENT SETS 0 6 0 Enter 3 for the number of DILUENTS and press the key The following screen appears METHOD02 CHANGED DILUT SAMPL VOL M FIRST VIAL TANK 1 21 VOLUME 100 50 25 MIX CYCLES 0 0 2 EQUIL TIME 0 0 0 ENTER SAMPLE VOLUME 0 0 2500uL Cd BS Sd Ee Oe E M Fill in the fields with the entries shown in the above screen As you move to and highlight each field a description of the information required for that field is displayed in the message line The information required for each data field is summarized here SAMPL VOL The volume of sample in the target vial in this example 0 before pressing FIRST VIAL First vial of a source set Diluent from the TANK is dispensed first Sample from the vial beginning the 2nd set in this example 1 is dispensed next Internal standard from the vial beginning the 3rd set in this example 21 is dispensed next VOLUME Volume of diluent 1st set or sample 2nd set or internal standard 3rd set MIX CYCLES Number of times the mixture in the target vials is mixed For best results set this value to 2 as shown in the screen above EQUIL TIME The amount of time mixture in target vial is allowed to equilibrate 9 95 Rev C 4 17 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisan
9. A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED D a gaa tia Contact us 888 88 SOURCE sales artisantg com www artisantg com LIQUID CHROMATOGRAPHY SERIES 200 AUTOSAMPLER User s Manual PerkinElmer precisely Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Series 200 Autosampler User s Manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Release History Part Number Publicatio
10. Guaranteed 888 88 SOURCE www artisantg com Installation Making Input Output Terminal Connections Series 200 LC Autosampler INJ 1 UVIVIS Detector Data System EXT ZERO RUN OUT O a EXT START E3 a Modular Analog Signal System Cable Series 200 LC Pump P N N291 0353 Figure 2 14 System Connection Diagram Series 200 LC Autosampler Series 200 LC Pump LC UV VIS Detector and a Data System Series 200 LC Autosampler INJ 1 LC 135C LC 235C E Data System Diode Array Detector o C STARTIN RUN OUT EXT ZERO A DATA RUN J7 i Modular or Analog Signal System Cable Series 200 LC Pump P N N291 0353 Figure 2 15 System Connection Diagram Series 200 LC Autosampler Series 200 LC Pump LC 235C Diode Array Detector and a Data System Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configunng the Autosampler Powering Up the Autosampler 3 2 The Default Top Level Main Screen 3 2 Reviewing the Default Performance Parameters 3 2 Defining System Parameters 3 5 Adding Method Options 3 6 Setting the Screen Contrast and Screen Saver 3 12 Setting the System Clock 3 13 Checking the Hardware Options tray type 3 14 Setting the Eror Recovery Mode 3 15 Defining the Commu
11. Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Dismantling the Injector Valve Reinstalling the Injector Valve in the Autosampler Note If you need to change the sample loop drain tubes pump or column tubes do so before reinstalling the Injector Valve Refer to the appropriate sections in this chapter 1 Refer to Figure 7 17 Valve mounting screw hole Red alignment dots Valve mounting screw hole Wrench Part No N293 1099 Injector valve stem pointing to 9 00 Pressure adjusting screw Figure 7 17 Orientation of Injector Valve Stem in the Injector Valve Assembly 2 Reattach the injector valve to the injector assembly using the two phillips head valve mounting screws you set aside in the procedure Removing the Injector Valve in this section Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Dismantling the Injector Valve Maintenance Using the Valve Wrench Part No N293 1099 realign the red dot on the outer lower ring with the red dot on the pressure adjusting screw as shown in Figure 7 17 Note that the wrench fits over the pressure adjusting screw This action sets the pressure rating of the injector valve Reinstall the valve actuator arm over the valve stem and lock it in place by tightening the two allen screws on the arm Usethe allen wrench provided Reinsert the stainless
12. Saving Changes to Screens When you finish making changes to a screen press the key to save those changes Changes you make to the default active method screens will remain in effect only until you turn off the power to the Autosampler To permanently store changes to the default method refer to Chapter 4 in this manual 1 16 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Instrument Specifications Introduction Instument Specifications Table 1 2 Specifications Injection range Programmable from 0 1 1 0 uL at 0 1 uL increments 1uL to2 5 mL at 1 uL increments Method parameters Injection volume sample draw speed flush volume flush cycle 2 external contacts start and stop time Methods Battery backed storage of up to 20 methods including time pro gramming Editing of stored methods possible during runs Injection cycle time 2 3 samples per minute Valve materials in contact with With Rheodyne 7725 for Analytical stainless steel Vespel sample ceramic PEEK With Rheodyne 9725 for Bio Applications PEEK Tefzel ceramic With Rheodyne 8125 for Microbore stainless steel Vespel ceramic PEEK Display 7 line by 40 character backlit liquid crystal display Communications Integrated communications for derivatization and dilution options Serial communications standard BCD interface standard 5 relay outputs 2 timed events 2 inject 1 start 1 17 A
13. 888 88 SOURCE www artisantg com Appendix B Changing the Performance Parameters The Default Performance Parameters se Acceptable Parameter Description Value or Range Sample Pump The speed at which the sample syringe MEDM Syringe Speed picks up and dispenses sample The for all speed varies with the size of the sample syringe syringe Note that for viscous samples sizes you should set the speed to VSLO very slow or SLOW Sample Pump The pause time following each sample 2 seconds 0 10 seconds Syringe Pause syringe movement This allows the air segment between the sample and the flush buffer to equilibrate Delay Time This is used for gradient methods It 0 00 0 00 999 minutes allows you to delay the sample injection minute by this amount of time to compensate for the dead volume between the pump and the Autosampler 7 8 Time Saver If this feature is enabled On it allows Off On Off the Autosampler to get the next sample and perform sample preparation before the other modules pump and detector are ready 9 Needle Level This determines how far the sample nee 1 99 dle goes into the sample vial The value corresponds to a percentage of the total distance from the shoulder top of the sample vial to the bottom of the sample holder For example 0 is at the bottom of the sample holder 99 is at the shoul der of the vial 10 Vent Mode If this feature is enabled On a volume of Off On air equal to the volume
14. 888 88 SOURCE www artisantg com Changing the Transfer Tubing Maintenance Changing the Transfer Tubing Removing the Injector Valve Cover Switch off the power to the Autosampler and disconnect it from the electrical supply 2 Refer to Figure 7 6 Open the sample compartment door sampling section of the Autosampler 3 Grasp the thumbscrews on the front of the needle cartridge and press up and slide the assembly to the right until it is over the sample tray as shown in Figure 7 6 4 Loosen the two thumbscrews holding the top cover over the injector valve assembly Slide the cover out from under the thumbscrews and lift the injector cover off the assem bly Sample compartment door Two thumbscrews holding the top cover over the injector valve Sample tray Upper thumbscrew on front of needle cartridge Lower thumbscrew on front of needle cartridge Figure 7 6 Removing the Injector Valve Cover 7 15 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Changing the Transfer Tubing pg Removing the Autosampler Cover Refer to Figure 7 7 Remove the three pairs of screws holding the cover on the Autosam pler There are a pair of screws located on the
15. Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Using the Keyboard and Display Include Hierarchy Diagram here as Figure 1 6 Screen Hierarchy Diagram 1 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Using the Keyboard and Display Introduction Keyboard Operations This section provides an overview of how to perform the following basic operations using the Autosampler keyboard e Selecting screen options e Selecting data fields and entering numeric information e Entering non numeric information into data fields e Scrolling through screen lists e Responding to screen prompts e Saving changes to screens 1 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Using the Keyboard and Display Selecting Screen Options Use the To Function Keys To select options displayed on a screen either as screen defined soft keys or as a list of items use the appropriate function key Once you press the appropriate function key the screen corresponding to the selected option appears Typical examples include selecting options from the top level Main Screen and the Configure Screen as shown here MAIN SCREEN METHODO0O0 STORED DEFAULT Screen defined soft keys gt gt IN LI I MN EEEN ICIS E GIN a EE aalGs To select one of the Main Screen options press t
16. Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance F4 Guidelines for Maintaining the Autosampler 7 3 Preventative Measuresto Avoid Problems 7 3 Routine Maintenance and Inspection 7 4 Viewing the Instrument Log 7 4 Replacing a Fuse 7 5 Locating and Removing the Power Input Module 7 5 Changing the Fuse s 7 6 Changing the Sample and Flush Syringes 7 8 Removing the Old Syringes 7 8 Loading the New Syringes 7 9 Replacing the Sample Needle 7 11 Changing the Transfer Tubing 7 15 Removing the Injector Valve Cover 7 15 Removing the Autosampler Cover 7 16 Replacing the Tubing 7 16 Flushing the System Replacing the Cover 7 18 Removing the Injector Valve 7 20 Removing the Injector Valve Cover 7 20 Disconnecting the Extemal Tubing Connections 7 20 Removing the Injector Valve Assembly 7 22 Dismantling the Injector Valve 7 24 Replacing the Rotor Seal and Stator Face Assembly 7 24 Reassembling the Injector Valve 7 26 Reinstalling the Injector Valve in the Autosampler 7 28 Changing the Sample Loop 7 30 Replacing the Tubing to the Column 7 31 Replacing the Tubing to the Pump 7 33 Replacing the Waste Tubes 7 35 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenanc
17. REMOTE INPUTS TEST COUNT 014 pect Joua1 Jouaz ine ft more Press F3 DYA to start the test The following screen appears PLACE TEST CONNECTOR ON J8 J15 SERIAL PRESS DUA2 DUA2 DUART TEST EXTERNAL ES OD E E E A Attach the loopback connector to the Serial A connector on the rear panel of the Autosam pler See Figure 6 2 Press F3 DA to continue If the test is successful the following screen appears DUA2 DUART TEST EXTERNAL PASS 01 FAIL 00 6 17 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Running the Diagnostic Tests DUART Test Extemal continued If a failure occurs the following screen appears DUA2 DUART TEST EXTERNAL U23 FAILURE PASS 00 FAIL 01 Repeat steps 1 3 above for Serial B connector 5 Press the key to redisplay page 2 of the Diagnostics Screen 6 18 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics Remote Input Test This test is designed to test the remote input In order to perform this test you need the Ready In Loopback connector Part No N293 0345 Plug the connector into the RDY IN ter minal connector on the Autosampler s I O Terminal Connector panel This panel is located on the right side of the Autosampler Refer to Figure 6 3 for location of th
18. brant and then making a series of vial to vial transfers Noinjections are performed between the vial to vial transfers An example is presented here to show you how to set up the Serial Dilution screen to per form a serial dilution starting with a single 1000 ug mL standard The dilution sequence is shown in Figure C 1 Serial Dilution Example In addition to the example there are two sections Operating Notes and Summary of Data Field Requirements to provide you with the information you need to customize your own serial dilutions To achieve the best performance use the default Performance Parameter settings To review or change the Performance Parameters refer to Appendix B Changing the Perfor mance Parameters Depending on the types of vial caps you are using you may need to change the Vent Mode parameter refer to Appendix E Operating Guidelines for Derivati zations and Dilutions Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix C Serial Dilutions Serial Dilution Example Serial Dilution Example Vial source Vial iL Diluent Tank iL Position Calibrant ug mL 1 E none E 1 1000 1 a 500 50 i 2 500 2 J 20 s0 i 3 100 3 J 20 s0 7 4 20 4 F 20 730 7 5 5 the Series 200 Autosampler Samples up to 225 Figure C 1 Serial Dilution Example Artisan Technology Group Quality Instrumentation Guara
19. gt 1 0 CT ES ES EEE Le ee Using the numeric keys on the keypad type in the time at which you want the first timed event to occur 0 2 is used in this example NOTE If you make a mistake entering numeric values in any of the fields on this screen press the key and retype the number To move to the T E 1 data field press the key To enable T E 1 press the 4 key YES will appear in the data field To move to and enable T E 2 repeat this step The following Timed Events Screen shows both T E 1 and T E 2 enabled METHOD0O0 CHANGED TIMED EVENTS EVENT TIME T E 1 T E 2 gt 1 0 2 YES YES CTi BS ES EEE ee ee To add another event press either the key or the key A second event will be added and the Time field for the next event will increment by 0 01 minute You can add up to 20 events using this process Go to the next page to continue Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining the Active Method All Options Enabled Setting Up Timed Events continued 5 The next screen shows what the Timed Events Screen looks like after you add several more events using the process described in step 4 METHOD0O0 CHANGED TIMED EVENTS EVENT TIME T E 1 T E 2 4 0 23 YES YES gt 5 0 24 YES YES 6 0 25 YES YES CN M A pres free To A ey eee To save these entries press the key This also takes you back to th
20. 1 Dilution Defining the Active Method All Options Enabled Refer to Figure 4 1 Thedilution tray is set up so that the first 40 vial positions are reserved for sample source vials and the second 40 vial positions 41 80 are reserved for target vials The screen entries and the procedure for this dilution scheme are on the facing page 500 uL of diluent is transferred from the Diluent Tank to target vial 41 100 uL of sample in sample vial 1 is transferred to target vial 41 For the second dilution 500 uL of diluent is transferred from the Diluent Tank to target vial 42 then 100 uL of sample in sample vial 2 is transferred to target vial 42 This process will continue until diluent and sample have been added to the remaining vials 43 through 80 A portion of the mixture specified in the VOLUME field in the Method Screen and shown on the facing page as 20 uL may be injected Whether or not the final dilution is injected and when the injection occurs depends on the DERIV DILUTE option selected from the Method Options Screen described in the Adding Method Options section in Chapter 3 of this manual OG p gt 7o Injection Valve DOOOODO Ge G gt Target Vials OE O EG 23 4 5 8 7 2 2 JJO 0000000 13 Sample Vials HOWO s a 43 9 Cs Figure4 1 Dilution Tray Setup for Pip
21. 2 6 Removing the Packing Foam 2 7 Making the Electrical Connections 2 8 Checking Electrical Power Requirements 2 8 Setting the Operating Voltage 2 8 Installing the Primary Fuse s 2 10 Installing the AC Line Cord 2 12 Making the Extemal Tubing Connections 2 13 Connecting the Drain Hosesto the Solvent Waste Container 2 14 Connecting the Autosampler to the Column 2 15 Connecting the Autosamplerto the Pump 2 15 Installing and Connecting the Sample Needle 2 17 Connecting the Flush Pump Syringe Tubing to the Solvent Container 2 20 Making Input Output Terminal Connections 2 21 Making a Connection between ModularCable and Twisted Pair Wires 2 22 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation This chapter describes how to install your Series 200 LC Autosampler It includes the fol lowing information e Warnings and Precautions e Preparing your laboratory e Unpacking your Series 200 LC Autosampler e Making the Electrical Connections e Making the External Tubing Connections e Making Input Output Terminal Connections Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Warnings and Precautions Wamings and Precautions Before installing the Series 200 LC
22. Autosampler read the following warnings and precau tions Solvents e Always use clean solvents Solvents which have been distilled in glass HPLC Grade are recommended e Filter the solvents and buffers through a 0 5 micron medium as an additional precau tion e Degas all aqueous and most organic solvents prior to use Note Mixtures of organics and water once degassed remain in a degassed state for longer periods of time than pure organics This minimizes bub ble formation in the transfer lines In general depending on your mobile phase strengths are 3 1 methanol water 1 1 methanol water or your specific mobile phase without buffer e Store flammable solvents or solvents which may form hazardous by products when the instrument is shut down by following the recommended shutdown procedure e Check compatibility of solvent s with the type of column s being used e Know the relative polarity and miscibility of the solvents being used Solvents with Low Boiling Points Do not use liquids that have a boiling point less than 30 C which have an auto ignition temperature Waming Do not use carbon disulfide or other solvents below 110 C Buffers Exercise care when using buffers in conjunction with organic solvents NEVER LEAVE BUFFERSIN THE SYSTEM OVERNIGHT Buffers left in the system can form salt crys tals which may cause premature injector valve failure and plug the transfer tubing and sam ple needle To re
23. Defining a Sequence 4 28 Defining a Prority Method 4 30 Defining a Random Priority Method 4 32 Chapter5 Running Methods Sequences or Priority Methods Getting Ready A Checklist 5 2 Powering Up the Autosampler 5 3 The Default Top Level Main Screen 5 3 Starting a Method 5 4 Starting the Active Method 5 4 Recalling and Starting a Stored Method 5 5 Starting a Sequence 5 7 Starting a Priority Method or Random Priority Method 5 8 Starting a Priority Method No Active Method or Sequence Running 5 8 Interrupting the Active Method or Sequence and Running the Priority Method 5 9 Chapter 6 Calibrations and Diagnostics Calibrating the Sample Needle 6 3 Accessing the Diagnostics Screen 6 6 Moving Between Pagesof the Diagnostics Screen 6 7 Running the Diagnostic Tests 6 8 Keypad Test 1 6 8 Keypad Test 2 6 9 Flash Test 6 10 RAM Test 6 11 Battery Backup Test 6 12 Peltier Communications Test 6 14 DUART Test Intemal 6 16 DUART Test Extemal 6 17 Remote Input Test 6 19 Output Relays Test 6 20 Read Firmware Part Number 621 ClearAll Methods 6 22 Clear Battery RAM 6 23 BCD Outputs Test 6 24 Viewing the Instrument Log 6 26 iii Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Chapter7 Maintenance Guidelines for Maintaining the Autosampler 7 3 Preventative Measuresto Avoid Problems 7 3 Ro
24. Possible Cause s Check and or remedy Problems Peak tailing Column overload Decrease the sample size 2 Increase column diameter Use higher capacity stationary phase Peaks are broad Injection volume too large Inject sample prepared in mobile phase with total sample volume lt 15 of volume of first peak Peak dispersion in injector valve Load air bubble before and after sample to decrease dispersion Sample overload 1 Dilute sample 1 10 and reinject 2 Inject a smaller sample volume Ghost peaks carryover Peaks from previous injection Use strong solvent after each run Column contamination Improve sample cleanup Wash sample needle guide Chemical interaction carryover or poor reproducibility may be due to certain compounds adsorbing to the transfer tubing or valve rotor seal 1 Adsorption to tranfer tubing Perform injections in the 10 to 20 uL range to ensure that excess sample and excess vol ume remain entirely in the sam pler needle Adsorption typically occurs with sample volumes gt 25 uL If this elimi nates the problem you can add the stainless steel holding loop 2 Adsorption to valve rotor seal If item 1 fails to eliminate prob lem try switching to PEEK rotor seal instead of Vespel Polynuclear aromatics are known to adsorb to Vespel e Poor area reproducibility e Good height Data handling system Check data handling component of LC syst
25. VOLUME TARGET MIX 1 TANK 500 2 0 gt 2 2 500 2 2 3 TANK 800 3 0 ENTER MIX CYCLES 0 9 Peer rank e __ s o si new a Once you complete the entries in Table C 1 you can start the serial dilution by pressing either F6 ERJ or F7 ENE NOTE The key and the key are disabled when the Serial Dilution screen is open F6 Ifyou press F6 the following prompt will appear in the message line PERFORM SERIAL DIL ONLY Y N N If you want the autosampler to perform the dilutions and stop press the 1 numeric key to change the default to Y If you press F7 the following prompt will appear in the message line PERFORM SERIAL DIL AND START Y N N If you want the autosampler to perform the dilutions and then start the active method or sequence press the 1 numeric key to change the default to Y Refer to Operating Notes and Summary of Data Field Requirements and Ranges for more information on the Serial Dilution option Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix C Serial Dilutions Operating Notes Operating Notes SERIAL DILUTION SCREEN SERIAL DILUTION TARGET VOL 0 TRANSFER SOURCE VOLUME TARGET MIX 1 TANK 500 2 0 carrot gt 2 2 500 2 2 3 TANK 800 3 0 ENTER MIX CYCLES 0 9 ETa Es u a M EA D EA To select a transfer entry from the list in the Serial Dilution screen press F 1 EE and enter the t
26. artisantg com Maintenance Changing the Sample and Flush Syringes Changing the Sample and Hush Synnges Removing the Old Syringes 1 Position the syringe plungers in the Dual Pump System for removal by using the PARK function on the Configure Screen as shown here MAIN SCREEN READY Ewer sea or oneal erry Press F7 from the Main Screen to move to the Configure Screen CONFIGURE SCREEN CONFIGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION LOG INSTRUMENT LOG inst commipiac Tcat_Jtoc J Prark f der Press F7 ESY to position the syringes for removal 2 Switch off the power to the Autosampler and disconnect it from the electrical supply 3 Refer to Figure 7 3 The Dual Pump System consisting of the Sample and Flush Syringes is located on the left side of the Autosampler 4 Pull the solvent filter at the end of the flush line tubing out of the solvent reservoir 5 Loosen the thumbscrew on the bottom of each syringe by turning it clockwise 6 Grasp each syringe barrel and loosen it by turning it clockwise 7 For the flush syringe unscrew the top of the syringe from the flush solenoid 8 For the sample syringe unscrew the top of the syringe from the T Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Changing the Sample and Flush Syringes Maintenance
27. key Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining the Active Method Samples Only 42 Procedure forModifying the Default Active Method Samples Only 4 3 Defining the Active Method All Options Enabled 4 5 Adding Calibrants 4 6 Setting Up Timed Events 4 9 Setting Up a Derivatization 4 11 Setting Up a Dilution 4 13 Creating New Methods 418 Creating a New Method from within the Active Method 4 19 Creating a New Method by Recalling a Method from the Directory 4 21 Editing a Stored Method 4 24 Defining a Sequence 4 26 Rules for Defining a Sequence 4 26 Identifying the Methods to Include in a Sequence 4 26 Procedure for Defining a Sequence 4 28 Defining a Priority Method 4 30 Defining a Random Priority Method 4 32 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences This chapter describes how to create Methods and Sequences on your Series 200 LC Autosampler It includes the following information e Defining the Active Method Samples Only e Defining the Active Method All Method Options Enabled e Creating New Methods e Editing a Stored Method e Defining a Sequence e Defining a
28. or one that is suitable for the in line device FROM PUMP amp TO COLUMN Zero dead volume union Scavanger column Figure 2 8 Connecting the Autosampler to a Pump 2 16 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making the External Tubing Connections Installation Table 2 7 Types of In Line Devices Between Pump and Autosampler Titinebevce Poeno Deion Zero dead volume Parker Hannifin Zero This union combines two pieces of 1 16 union Dead Volume Union inch tubing for a zero dead volume union PerkinElmer Part No This type of union makes it easy for you 0990 3289 to disconnect the Autosampler from the pump Scavanger Column PerkinElmer Scavanger Scavanger columns are placed between Column Kit the pump and the Autosampler to protect Part No 0258 0204 the analytical column from particulate matter and any absorbed contaminants in the mobile phase In line solvent filter PerkinElmer In Line This filter is similar to the Scavanger Col Solvent Filter System umn Part No 0990 3606 Installing and Connecting the Sample Needle 1 Locate the following items in the appropriate startup kit The part numbers for each of the available start up kits are listed in Table 2 8 for your convenience Table 2 8 Parts Needed for Installing and Connecting the Sample Needle SS Startup Kit Bio Startup Kit Qty Description N293 0305 N 293 030
29. other end of the flush line tubing is already con nected to the flush pump syringe 2 Place the solvent filter into the solvent reservoir Flush line tubing Solvent filter Tubing going to sample needle Flush syringe Sample syringe Figure 2 11 Connecting the Flush Line Tubing to the Solvent Container 3 Apply power to the Autosampler 4 Press the key on the keyboard and flush solvent through the system until there are no bubbles in the sample tubing Toinsure that there are no bubbles inspect closely the tubing near the point where it enters the Tefzel union Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making Input Output Terminal Connections Installation Making Input Output Terminal Connections The input output terminal on the Series 200 LC Autosampler uses phone jack connectors for quick contact closure connections between instruments Figure 2 12 shows the input output terminal connectors on the right side panel of the Series 200 Autosampler Table 2 9 pro vides a description of each input output connector Nec PIN Insc ret ee ec Note The RDY IN 2o E connection is required STRT pi JTE2 for operation ON O OFF Figure 2 12 The Seri
30. produces large AC line voltage fluctuations install a voltage regulator between the AC line voltage outlet and the Series 200 LC Autosampler Instrument grounding is required and is accomplished by a third wirein the line voltage box If your electrical outlet does not contain a ground then contact your local electrical con tractor 2 Setting the Operating Voltage The power input module located on the rear panel contains an outlet for the AC line cord the voltage selector card and the system fuse s Inside the power input module is a small voltage selector card that you need to set to the local line voltage The selected voltage is indicated by a white dot in the power input module cover next to the displayed voltage Table 2 4 shows the setting to use for various line voltages Table 2 4 Voltage Selector Settings AC Voltage Range AC Voltage Tap Set Voltage Selector To Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making the Electrical Connections Installation To set the Series 200 LC Autosampler operating voltage 1 2 Make sure the main power switch is in the OFF position Refer to Figure 2 2 Remove the power input module cover by inserting a small flat blade screwdriver into the notch and carefully pry off the cover Remove the voltage selector card from the power input module 100 Voltage Selector Arrow Voltage Sele
31. rating and plug appropriate for your location See Figure 2 4 for the correct cord 1 Select the proper line cord for your location 2 Insure that the power switch is off and insert the line cord plug into the AC supply North America a a Standard NEMA 5 15 india 0999 1420 0999 1423 Europe British Standard ve CEE 7 BS 1363 Schuko United Kingdom 0999 1415 0999 1414 Europe Australia Switzerland ETSA S 86 0999 1413 0999 1417 0999 1422 0999 1424 Denmark Japan 0999 1416 1 0999 1425 Figure 2 4 2 12 AC Line Cord Selections Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making the External Tubing Connections Installation Making the Extemal Tubing Connections This section contains procedures describing how to make all the necessary external tubing connections and includes Connecting the drain hoses to the solvent waste container Connecting the Autosampler to the column Connecting the Autosampler to the pump Installing and connecting the sample needle uF WN Connecting the flush pump syringe tubing to the solvent container For your convenience all of the required internal tubing connections to the injector valve of the Series 200 LC Autosampler are made at the factory If you need to change the sample loop replace tubing or connectors refer to Chapter 7 in this manual Figure 2 5 provides an overview of typica
32. steady stream of solvent If there is no solvent flow replace the needle as described in Chapter 7 of this manual Use correct recommended vials and caps Clear obstruction If thereis no obstruction call PerkinElmer service Use correct recommended vials and caps If this fails call PerkinElmer service Vial missing Incorrect vial size or missing vial Use correct recommended vials and caps 8 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendixes Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix A Sample Trays Available Tray Types You can select from one of the following eight different sample trays Description Part No Standard 100 Vials N293 1007 225 Vials N 293 0043 205 Vials N 293 0042 85 Vials N 293 0041 Diluent 80 Vials 1 Tank N293 0314 Peltier 100 Vials N293 0310 Microtiter 96 24 Vials N 293 0045 Each of the following sections contains a description and diagram of the available sample trays Standard 100 Part Number N293 1007 Accepts 1 1 8 mL vials 80 P N N930 7017 2 0 2 mL Microvials 80 P N N930 2136 Requires Glass Sleeve P N N930 7027 Og Artisan Technology Group Quality Instrumentation Guara
33. test is designed to test the internal DUART which controls serial communications The DUART is configured for local loop back mode Data bytes 55 and AA aretransmitted This test verifies that bytes 55 and AA are received If a failure occurs call your PerkinElmer Service Engineer PAGE 2 OF THE DIAGNOSTICS SCREEN PELT PELTIER COMMUNICATIONS TEST DUA1 DUART TEST INTERNAL DUA2 DUART TEST EXTERNAL INP REMOTE INPUTS TEST COUNT 01 CESA ouarfouazpne Pf A E Press F2 to start the test The following screen appears during the test DUA1 DUART TEST INTERNAL PASS 01 FAIL 00 2 If a failure occurs a screen similar to the following appears DUA1 DUART TEST INTERNAL PASS 00 FAIL 01 Press the key to redisplay page 2 of the Diagnostics Screen 6 16 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics DUART Test Extemal This test is designed to test DU ART and connections between the Serial A and Serial B ports on the rear panel of the Autosampler See Figure 6 2 for the location of the connectors Ifa failure occurs call your PerkinElmer Service Engineer Note You cannot run this test without a special test fixture loopback connector PAGE 2 OF THE DIAGNOSTICS SCREEN PELT PELTIER COMMUNICATIONS TEST DUA1 DUART TEST INTERNAL DUA2 DUART TEST EXTERNAL INP
34. the end of a Flush Cycles method 16 Hard Key Cycles The number of times the flush syringe will cycle when you press the key 17 Reagent Mixing The speed of the flush syringe when it Speed In picks up sample plus reagent from the vial through the sample needle for mix ing Note that for viscous samples you should set the speed to VSLO very slow or SLOW 18 Reagent Mixing The speed of the flush syringe when it Speed Out ejects sample plus reagent through the sample needle back into the vial for mix ing Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining System Parameters Configuring the Autosampler Defining System Parameters This section describes how to make changes to the Autosampler s system parameters and includes e Adding Method Options e Setting the Screen Contrast and Screen Saver e Setting the System Clock e Checking the Hardware Options tray type e Setting the Error Recovery Mode You can perform all of these functions from the Instrument Setup Screen The procedure for accessing the Instrument Setup Screen is summarized here for your convenience DEFAULT MAIN SCREEN READY E E sea on A perry From the Default Main Screen press F7 to move to the Configure Screen CONFIGURE SCREEN CONFIGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION LOG INSTRUMENT LOG inst cou
35. this example 1 METHODOO STORED METHOD NAME LAST MODIFIED DEFAULT 02 FEB 1994 12 00 carrot UTM TEST 02 FEB 1994 13 00 l 2 UTM ASSAY 03 MAR 1994 12 00 Message line STORE STOR OR RECALL RCL A METHOD Ci f sf fro _f__fstor __ Press F4 and the message line will change to the following RECALL METHOD 1 Press the key and the message line will display the following prompt OVERWRITE ACTIVE METHOD Y N N Press the 4 numeric key to change the default to Y This will replace the current active method which is Method00O with Method01 from the directory The Main Screen will appear as shown on the next page Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Sequences Starting a Method MAIN SCREEN METHODO1 STORED UTM TEST READY E E u E EA S Gad At this point press the key on the keyboard and the screen will change to display status information about the new current active method method01 METHOD01 STORED UTM TEST SAMPLE VIAL 1 REP 1 OF 2 PIPETTING SAMPLE E CS ee E Ee Ee a Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Starting a Sequence Running Methods and Sequences Starting a Sequence This section describes how to start a sequence MAIN SCREEN METHODOO READY E C BS Be Gd Press F5 IEZJ from the Main Screen to go to the Sequence Scr
36. use the numeric keys to type in a new value If the method already exists the message line will display the following prompt OVERWRITE EXISTING METHOD Y N Use the 1 0 numeric keys to respond The message line changes and asks you if you want to name the method as shown in the next screen METHOD0O0 CHANGED DEFAULT FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 Message line NAME METHOD Y N N mM E ESO I BS Ss es 3 To name the method press the numeric key The screen shown on the next page appears 4 19 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Creating New Methods Creating a New Method from within the Active Method continued METHOD01 STORED ABCDEFGHIJ USE CURSOR AND SEL TO KLMNOPQRST SELECT BKSP TO BACKSPACE UVWXYZ PRESS ENTER WHEN COMPLETE 0123456789 TD E E E E Using the cursor arrow keys highlight the letter or number then press F5 to enter that choice You can select up to eight characters Press F3 EZS to erase a selected letter or num ber As you enter the name the screen changes to reflect the highlighted name as shown in the next screen METHOD01 STORED ABCDEFGHIJ USE CURSOR AND SEL TO KLMNOPQRST SELECT BKSP TO BACKSPACE UVWXYZ PRESS ENTER WHEN COMPLETE 0123456789 UTM ASSAY ee 0 ee ee ee ee 5 Press the key to save the name and method number in the Auto
37. vial caps include Description Part No Blue 11 mm Silicone Teflon vial septa N 930 7022 Blue 11 mm Silicone Teflon vial with cross cut septa N 930 7021 Red 11 mm Screw Cap Silicone Teflon vial with cross cut septa N930 7019 When using Polyethylene Snap Caps Part No N930 7023 set the Vent Mode to OFF When performing dilutions denivatizations or serial dilutions Verify or set the following Performance Parameters to the values modes listed here Performance Parameter Valueor Mode Air Cushion 10 uL Reagent Mixing Speed In SLOW Reagent Mixing Speed Out FAST Also remember to set the number of mix cycles to 2 for your derivatization or dilution method Using these conditions you can expect a vial to vial reproducibility of 1 2 RSD for dilu tions in the 1 2 through 1 50 range Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix E Operating Guidelines for Derivatizations and Dilutions Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Index Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Index Active method see also Method defined P 3 interrupting with a priority method 5 9 sample injection order samples and calibrants 4 8 s
38. 00uL Eo E N O E M M Using the numeric keys enter the approximate initial volume of liquid present in the sample vials This volume should correspond to the amount of liquid present before any reagent or liquid has been transferred to the sample vial To accept the entry and move to the next field VIAL use the cursor arrow keys Go to the next page to continue 9 95 Rev C 4 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining the Active Method All Options Enabled Setting Up a Denvatization continued As you move to and highlight each field a description of the information required for that field is displayed in the message line Use the numeric keys to enter the information for each of the reagents The information required and the acceptable range for each of the fields in the Method Derivatization Screen is summarized here SAMPL VOL Initial volume of liquid present in the sample vial 0 2500 uL VIAL Reagent vial number 1 225 VOLUME Volume of reagent to be transferred to the sample vial 0 2500 uL MIX CYCLES The number of times cycles the mixture sample 0 9 and reagent will be mixed after reagent transfer Unless a specific chemistry requires it best results are obtained by adding reagents and performing two mixing series REACT TIME Reaction time for the mixture 0 99 Min The following example shows a completed M
39. 4 21 4 22 Customer service P 5 D Defining system parameters 3 5 accessing the Instrument Setup Screen 3 5 adding method options 3 6 Derivatization see also Method Derivatization Screen adding the deriv dilute option 3 8 defined 3 8 Derivatization Dilution adding the deriv dilute option 3 8 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Index Diagnostic Screen 6 6 6 7 accessing 6 6 pages 6 7 Diagnostic tests 6 8 6 24 Battery Backup Test 6 12 6 13 BCD Outputs Test 6 24 Clear All Methods 6 22 Clear Battery RAM 6 23 DUART Test External 6 17 6 18 DUART Test Internal 6 16 Flash Test 6 10 Keypad Test 1 6 8 Keypad Test 2 6 9 Output Relays Test 6 20 Peltier Communications Test 6 14 6 15 RAM Test 6 11 Read Firmware Part Number 6 21 Remote Input Test 6 19 Dilution see also Method Dilution Screen adding the deriv dilute option 3 8 defined 3 8 sample tray example 4 14 4 16 setting up the sample tray 4 13 4 14 Directory viewing methods stored in 4 27 storing new methods in 4 20 Display description 1 3 1 4 1 8 Drain hoses connecting to the waste container 2 14 location 2 14 Dual syringe system description 1 6 Editing a method 4 24 4 25 Error codes in the Instrument log 6 27 Error messages in the display 8 10 Error recovery defining the error recovery mode 3 15 F Firmware determine firmware part number 6 21 Flush syringe changing the flush syringe 7 8
40. 6 Part Numbers Part Numbers Needle Cartridge N293 1012 Needle Cartridge Thumbscrews 0992 0672 Slotted Needle Nut N 293 1064 Union 0990 3289 Union Holder N293 1081 Screw M3 X 6 0991 9200 Clamp 0998 4738 PEEK Nut 0992 0513 Needle Spring 0992 0429 a 9 95 Rev C 2 17 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Making the External Tubing Connections 2 Lift the needle assembly up and move it over the injector valve port The location of the injector valve port is shown in Figure 2 9 Injector valve port Figure 2 9 Location of the Injector Valve Port The following steps are keyed to Figure 2 10 3 Slide the needle spring over the curved top portion of the sample needle as shown in Figure 2 10 Slide one of the PEEK nuts over the end of the sample needle as shown 5 Thread the PEEK nut on the sample needle into the top portion of the union 6 Slidethe union holder up over the sample needle and union Makesure the union holder is oriented as shown facing forward 7 Insert the sample needle union holder assembly into the needle cartridge 8 Toholdthesample needle in place thread and tighten the slotted needle nut into the top of the needle cartridge as shown 9 Press and hold the needle cartridge up against the face plate so that the lower surfaces of the two plates are flush While holding the needle cartridge securely in pla
41. AIR CUSHION you need to enter or key NEEDLE LEVEL 5 EXCESS VOL press you need to make for TIME SAVER Off each highlighted data field SELECT LOOP MODE PARTIAL FIXED _ ee ee ee ee ee Screen defined soft keys F2 these change depending on the data field highlighted To change the LOOP MODE from 1 the default partial to fixed Press F2 EEO INJECTION PERFORMANCE PARAMETERS INJECTION PERFORMANCE PARAMETERS LOOP MODE fixed VENT MODE DELAY TIME yw 0 01 AIR CUSHION NEEDLE LEVEL 5 EXCESS VOL TIME SAVER Off ENTER DELAY TIME 0 01 999 MINUTES EE TT For data fields requiring numeric entries use the numeric keys on the keypad to type in new values The acceptable range for any field requiring a numeric entry is shown in the message line For example for DELAY TIME you could enter in any value within the acceptable range 0 01 999 minutes To accept the entry and move down to the next parameter use the cursor arrow keys To go to page 2 Sample Pump Reagent Mixing Performance Parameters press F8 DRG To accept and save the change s and return to the Instrument Setup Screen press the key To cancel changes and return to the Instrument Setup Screen press the key Refer to Table B 2 on the next page It lists the names of each of the default performance parameters in Table B 1 and their corresponding names as they appear on each of the three pages making up the Performance Parame
42. ATE 0 0 0 CE N _ _ Press F1 until the O STATE value for the DATA output is highlighted Then press F2 to change the 0 to 1 The DATA value must be set to 1 in order to activate the other lines Press F1 again to highlight the O STATE value for the line 80 output Then press F2 to change the 0 to 1 Check the DVM to verify the change in state Repeat this procedure for all outputs Press the key to redisplay page 3 of the Diagnostics Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics Serial Ports BCD Port Figure 6 4 Rear Panel of the Autosampler Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Viewing the Instrument Log Viewing the Instrument Log The following procedure describes how to access and view the Instrument Log MAIN SCREEN READY E E sea on Ponral erry From the Main Screen press F7 to move to the Configure Screen CONFIGURE SCREEN CONFIGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION LOG INSTRUMENT LOG inst commipiac Tcat_Jtoc J Jrark ver 2 Press F5 to move to the Instrument Log Screen This displays the number of valve cycles and injection cycles performed by the A
43. C Autosampler inert to the most common mobile phases that are used for biological assays If you have questions about your mobile phase contact a PerkinElmer representative Refer to Table 2 2 Caution Aqua Regia and sulfuric acid are known to A dissolve titanium Table 2 2 Solvents Which May Corrode the Series 200 Bio LC Autosampler Aqua Regia 80 HCI 20 HNO3 Anhydrous M ethanol Hydrogen Peroxide Chlorinated solvents Dichloromethane Sulfuric Acid Conc Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Warnings and Precautions Solvents with Auto Ignition Temperature Below 110 C Certain solvents have a temperature at which they are combustible upon contact with a heated surface even in the absence of a spark or other source of ignition A list of common solvents with their auto ignition temperatures arein Table 2 3 Waming Do not use carbon disulfide or other solvents which have an auto ignition temperature below 110 C Table 2 3 Auto I gnition Temperatures of Common LC Solvents Solvent Auto I gnition Temp C C C Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preparing Your Laboratory Installation Prepanng Your Laboratory Before installing your Series 200 LC Autosampler prepare your laboratory according to the following guidelines Required Air Quality To minimize contami
44. Checking the Hardware Options tray type The Tray Type screen provides information about the type of sample tray that is installed in the Series 200 LC Autosampler This screen is for information only and does not contain any soft function keys 4 From the Instrument Setup Screen press F5 to move to the Hardware Options Screen The type of tray installed in the Autosampler will be displayed on the screen as shown TRAY TYPE SCREEN TRAY TYPE STANDARD 100 VIALS 2 To return to the Instrument Setup Screen press either the key or the key The following is a list of the available tray types in addition to the STANDARD one that EJ may appear in the Tray Type Screen 225 VIALS DILUENT 80 VIALS TANK 25 VIALS PELTIER 100 VIALS 205 VIALS 85 VIALS MICROTITER 96 24 VIALS 3 14 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining System Parameters Configuring the Autosampler Setting the Enor Recovery Mode This Error Recovery Screen allows you to specify how the Series 200 LC Autosampler responds to a missing sample vial From the Instrument Setup Screen press F6 to move to the Error Recovery Screen ERROR RECOVERY SCREEN ERROR RECOVERY SAMPLE VIAL MISSING CONTINUE NG CL A A E 2 Press F1 if you want the Autosampler to resume and continue processing sample vials if it encounters a missing sample vial Press F2 if you want the Autosa
45. Connector Panel 1 4 Sample Tray 1 4 Sample Needle Assembly 1 4 Dual Syringe System 1 6 RearPanel 1 7 Using the Keyboard and Display 1 8 Summary of the Keyboard Functions 1 9 Keyboard Operations 1 11 Instrument Specifications 1 17 Chapter 2 Installation Wamings and Precautions 2 2 Solvents 2 2 Solvents with Low Boiling Points 2 2 Buffers 2 2 Conosion 2 3 Solvents with Auto Ignition Temperature Below 110 C 2 4 Preparing Your Laboratory 2 5 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Required Air Quality 2 5 Adequate Bench Space 2 5 Waste Solvent Disposal 2 5 Unpacking Your Series 200 LC Autosampler 2 6 Items Included 2 6 Removing the Packing Foam 2 7 Making the Electrical Connections 2 8 Checking Electrical Power Requirements 2 8 Setting the Operating Voltage 2 8 Installing the Primary Fuse s 2 10 Installing the AC Line Cord 2 12 Making the Extemal Tubing Connections 2 13 Connecting the Drain Hosesto the Solvent Waste Container 2 14 Connecting the Autosamplerto the Column 2 15 Connecting the Autosamplerto the Pump 2 15 Installing and Connecting the Sample Needle 2 17 Connecting the Flush Pump Syringe Tubing to the Solvent Container 2 20 Making Input Output Terminal Connections 2 21 Making a Connection between Modular Cable and Twisted Pair Wires 2 22 Chapter 3 Config
46. D BRANCHER LE CORDON D ALIMENTATION AVANT D OUVRIR DA CONTINUIT DES MASSES EST ESSENTIELLE POUR UN FONCTIONNEMENT SANS DANGER NE J AMAIS UTILISER L QUIP MENT SI LE FIL DE TERRE N EST PAS RACCORD Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Safety and Regulatory Information Elec tical Wamings f Waming f Waming f Waming f Waming Electrical Warnings Connect the instrument to an AC line power outlet that has a protective ground connec tion To ensure satisfactory and safe opera tion of the instrument it is essential that the protective ground conductor the green yel low lead of the line power cord is connected to true electrical ground Any interruption of the protective ground conductor inside or outside the instrument or disconnection of the protective ground terminal may impair the protection provided by the instrument Do not operate the instrument with any cov ers or parts removed Do not attempt to make adjustments replace ments or repairs to this instrument except as described in the accompanying User Documen tation Only a Perkin Elmer service representa tive or similarly trained and authorized person should be permitted to service the instrument Use only fuses with the required current rat ing and of the specified type for replacement Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www
47. Engineer Note You cannot run this test without a special test fixture loopback connector PAGE 2 OF THE DIAGNOSTICS SCREEN PELT PELTIER COMMUNICATIONS TEST DUA1 DUART TEST INTERNAL DUA2 DUART TEST EXTERNAL INP REMOTE INPUTS TEST COUNT 01 fevt ouatfouazfine fore Press F1 to start the test The following screen appears PELT PELTIER COMMUNICATIONS TEST PLACE TEST CONNECTOR ON J4 PELTIER PRESS PELT CENA E E E E A E 2 Attach the loopback connector to the Peltier J4 connector on the rear panel of the Autosampler See Figure 6 2 Press F1 to continue If the test is successful the following screen appears PELT PELTIER COMMUNICATIONS TEST PASS 01 FAIL 00 6 14 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics Peltier Communications Test continued If a failure occurs the following screen appears PELT PELTIER COMMUNICATIONS TEST PELTIER COMMUNICATIONS FAILURE PASS 00 FAIL 01 Press the key to redisplay page 2 of the Diagnostics Screen Serial Ports Serial Port Peltier J4 connector Connectors A and B Figure 6 2 Rear Panel of the Autosampler 6 15 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Running the Diagnostic Tests DUART Test Intemal This
48. IGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION LOG INSTRUMENT LOG inst comm orac can oc earn oer Press F7 ENI to move the Sample Needle Assembly over the injector valve port 2 Switch off the power to the Autosampler and disconnect it from the electrical supply 7 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Replacing the Sample Needle 3 Refer to Figure 7 4 Remove the needle cartridge from the needle cartridge face plate by removing the two thumbscrews Needle cartridge face plate L Thumbscrews Needle cartridge Figure 7 4 Removing the Needle Cartridge Steps 4 9 below are keyed to Figure 7 5 4 5 10 11 Remove the PEEK nut connecting the sample tubing to the union Remove the slotted needle nut holding the sample needle and union holder in the needle cartridge Remove the sample needle union and union holder from the cartridge slide the union holder off the sample needle Unscrew the PEEK nut connecting the sample needle to the union and remove the PEEK nut from the needle Slide the needle spring over the curved top portion of the sample needle and set aside Replace the sample needle Reverse the above steps to install the new needle Note When reattaching the needle cartridge to the faceplate as shown in the lower p
49. ION TARGET VOL 0 TRANSFER SOURCE VOLUME TARGET MIX gt 1 TANK m 500 0 ENTER TARGET VIAL 1 225 amp TANK ET Ed ES A Using the numeric keys type in target vial 2 for the first transfer The entry for the Mix data field should be left as 0 for the first transfer entry The completed screen for the first transfer is shown next SERIAL DILUTION TARGET VOL 0 TRANSFER SOURCE VOLUME TARGET MIX gt 1 TANK 500 2 0 ENTER MIX CYCLES 0 9 ET Es BG M A E A To add the next transfer entry press either the key at the last data field or the key Modify transfer entry 2 and then add and modify the remaining transfer entries required to accomplish the serial dilution shown in Figure C 1 Since only three transfer entries can be dis played on the screen at one time the eight transfer entries you need to make are listed inTable C 1 below for your convenience Table C 1 Sample Dilution Screen Entries SERIAL DILUTION TARGET VOL 0 TRANSFER SOURCE VOLUME TARGET MIX TANK 500 2 500 TANK 800 2 200 TANK 800 3 200 TANK 750 4 250 ANoOoaRWND anak BwWWD PP NONONONO IMPORTANT Make sure and complete all additions to each vial in the order shown in Table C 1 before proceeding to the next vial Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Setting up the Serial Dilution Screen Appendix C Serial Dilutions SERIAL DILUTION TARGET VOL 0 TRANSFER SOURCE
50. IRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 ENTER FIRST VIAL NUMBER 1 225 mM eed EN Bd E ET es Press F2 and the Directory Screen shown in step 1 reappears with the following prompt in the message line COPY METHOD 0 FOR EDITING Using the cursor arrow keys move down through the list of stored methods until the carrot gt is next to the method you want to copy this example will use Method 1 To accept press the key The Directory Screen then prompts you to overwrite the method Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Editing a Stored Method Creating Methods and Sequences Editing a Stored Method continued METHODO1 STORED METHOD NAME LAST MODIFIED DEFAULT 02 FEB 1994 12 00 UTM TEST 02 FEB 1994 13 00 2 UTM ASSAY 03 MAR 1994 12 00 OVERWRITE EDIT METHOD Y N N To overwrite the selected method press the 4 numeric key As soon as you press this key the following Edit Screen will appear You will note that except for the function the Edit Screen is identical to the Method Screen EDIT SCREEN EDIT 01 STORED UTM TEST FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 ENTER FIRST VIAL NUMBER 1 225 Soft Keys pe TST Reel ESC BGT B ECH m Use the same procedures described in the Defining the Active Method All Options Enabled section in this chapter to make changes to the method Note 1 If you are making cha
51. Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Table 2 General Instrument Problems General Instrument Problems Short injector valve rotor seal life Short sample or flush syringe life Injection valve mechanism is noisy or injection valve error is displayed Possible Cause s Dirty mobile phase Dirty sample Incorrect vial caps Dirty flush solvent Injection valve is worn Troubleshooting Check and or remedy Replace inlet solvent filter with recommended filter Use only degassed and filtered HPLC grade solvents for flushing solu tion and mobile phase Improve sample cleanup Use recommended vial caps Replace inlet solvent filter with recommended filter Use only degassed and filtered HPLC grade solvents for flushing solu tion and mobile phase Rebuild injection valve as described in Chapter 7 of this manual If the valve appears to be working properly call Perkin Elmer service Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Troubleshooting Table 3 Display Error Messages Troubleshooting Table 3 Display Enor Messages Possible Cause Check and or remedy Flush pump failed Needle blocked X motor failed or Inappropriate vial or cap size Y motor failed Obstruction in sample tray Z motor failed Inappropriate vial or cap size Remove needle and press flush and check for
52. MED EVENTS on DERIV DILUTE on METHOD EDIT off RANDOM SAMPLING off SERIAL DILUTION off SELECT STANDARDIZE OPTION ON OFF K A E A E A E When the above method options are enabled the top level Method Screen displays them as the Bi GW and soft function keys as shown here METHOD SCREEN METHOD STORED FIRST LAST VOLUME REPLICATES TIME 1 1 10 1 10 ENTER FIRST VIAL NUMBER 1 225 M u E Sd BS Ea es Each of these method options is described in a separate procedure within this section as indi cated here Method Option Method Screen Procedure Function Key STANDARDIZE STD Adding Calibrants TIMED EVENTS T E Setting up Timed Events DERIV DILUT DRV Setting up a Derivatization DERIV DILUT DIL Setting up a Dilution The DIL function key is displayed when the special dilution tray is inserted in the Autosampler Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining the Active Method All Options Enabled Adding Calibrants This section describes how to add calibrants STANDARDIZE option enabled to the method METHOD SCREEN METHODO0O0 STORED FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 Message line ENTER FIRST VIAL NUMBER 1 225 mM E EA Bd A En e From the Method Screen press F3 to move to the Method Standardize Screen The data field for the Total Calibrants is highlighed METHOD STANDARDIZE SCREEN
53. METHOD00 STORED STANDARDIZE TOTAL CALIBRANTS 0 Message line ENTER TOTAL CALIBRANTS 0 7 Using the numeric keys on the keypad type in the total number of calibrants as directed by the message line In this example 5 is used Press the down cursor arrow key to accept the number of calibrants The screen will change to display the correct number of VIAL and VOL columns 5 and the available soft function keys as shown here Note that the data field for the first VIAL is highlighted METHOD0O0 CHANGED STANDARDIZE TOTAL CALIBRANTS 5 REPS 1 INTERVAL 4 VIAL 1 2 3 4 5 VOL 5 5 5 5 5 ENTER CALIBRANT VIAL NUMBER 1 225 ECN CES int fo fvot ft Use the numeric keys to change the vial number and the cursor arrow keys to move between vial numbers In this example the vial numbers have been changed as shown on the next page Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Active Method All Options Enabled Creating Methods and Sequences Adding Calibrants continued METHOD01 STORED STANDARDIZE TOTAL CALIBRANTS 5 REPS 1 INTERVAL 4 VIAL 10 11 12 13 14 VOL 5 5 5 5 5 ENTER CALIBRANT VIAL NUMBER 1 225 EN EES int To a ff 5 To select the next field press the corresponding soft function key For example to change the number of replicates press F2 BEA and the data field for Reps becomes highlighted The message line changes instructing you to en
54. NG SAMPLE E CS ee E Be ES a Interrupting a Sequence If the Autosampler is performing an operation on a method like sampling within a sequence when you press the key the following Main Screen display appears The PPEND in the top line denotes that a priority method is pending and will start as soon as the Autosampler com pletes its current operation The sequence will resume once the priority method is completed METHOD03 STORED LINKED PPEND UTM SET 1 SAMPLE VIAL 1 REP 1 OF 2 PIPETTING SAMPLE E CS ee E Be a Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Sequences Notes Notes 5 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Calibrating the Sample Needle 6 3 Accessing the Diagnostics Screen 6 6 Moving Between Pages of the Diagnostics Screen 6 7 Running the Diagnostic Tests 6 8 Keypad Test 1 6 8 Keypad Test 2 6 9 Flash Test 6 10 RAM Test 6 11 Battery Backup Test 6 12 Peltier Communications Test 6 14 DUART Test Intemal 6 16 DUART Test Extemal 6 17 Remote Input Test 6 19 Output Relays Test 6 20 Read Firmware Part Number 6 21 ClearAll Methods 6 22 Clear Battery RAM 6 23 BCD Outputs Test 6 24 Viewing the Instrument Log 6 26 Artisan Technology Group Quality Instrumentation Guarante
55. OSTICS SCREEN DUA1 DUART TEST INTERNAL DUA2 DUART TEST EXTERNAL INP REMOTE INPUTS TEST COUNT 01 C CONE ES A TT PAGE 3 OF THE OUT OUTPUT RELAYS TEST DIAGNOSTICS SCREEN pART READ FIRMWARE PART NUMBERS CLRM CLEAR ALL METHODS CRAM CLEAR BATTERY RAM BCD BCD OUTPUTS TEST COUNT 01 M CSG ST Ee ME ETS To move from page 3 of the Diagnostics Screen to page 4 press F8 MA NOTE Page 4 of the Diagnostic Screen is for use by PerkinElmer Service Personnel only PERKIN ELMER PERSONNEL ONLY COUNT 01 a EC Ped Bad a ee ES 4 The sections that follow describe how to run each of the diagnostic tests from each page of the Diagnostic Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Running the Diagnostic Tests Running the Diagnostic Tests Keypad Test 1 This test is designed to test the general functionality of the keypad If a number is displayed next to pass the keypad passed the test If a number is displayed next to fail call your PerkinElmer Service Engineer PAGE 1 OF THE DIAGNOSTICS SCREEN KPD1 KEYPAD DISPLAY TEST 1 KPD2 KEYPAD DISPLAY TEST 2 FLSH FLASH TEST RAM RAM TEST BBUP _ BATTERY BACKUP TEST COUNT 01 E kroz rush Pram Peeve A Press F1 to start the test If the test is successful the following screen appears KPD1 KEYPAD DISPLAY TEST 1 PASS FAIL eect fous fousz
56. P 2 on power up 3 2 5 3 using with keyboard 1 8 1 12 Method creating a new method from within the active method 4 19 4 20 creating a new method from within the directory 4 21 4 22 defined P 2 editing a method 4 24 4 25 recalling and starting a stored method 5 5 see also Active method Method Derivatization Screen 4 11 summary of the data field requirements and ranges 4 12 Method Dilution Screen 4 15 4 17 summary of the data field requirements and ranges 4 15 4 17 Method edit adding the method edit option 3 9 see also Method Edit Screen Method Edit Screen 4 24 Method Screen accessing 4 3 all options enabled 4 5 summary of the data field requirements and ranges Samples only 4 3 Method Standardize Screen 4 6 summary of the data field requirements and ranges 4 7 Needle cartridge installing 2 17 2 19 removing 7 12 Artisan Technology Group Quality Instrumentation Index Operating voltage selecting 2 8 2 9 Packing foam removing 2 7 Peltier Accessory 3 20 setting the temperature 3 20 Performance Parameters See Configuring the Autosampler Power input module location 1 7 removing to change fuses 7 5 setting the operating voltage 2 8 Power requirements 1 17 2 8 Powering up the Autosampler 3 2 5 3 precision 1 17 Preparing Y our Laboratory 2 5 bench space requirements 2 5 reguired air quality 2 5 waste solvent disposal 2 5 Priority method defined P 3 setting up a priority method 4 31 starting 5 8 when torun a
57. Priority Method e Defining a Random Priority Method Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining the Active Method Samples Only Defining the Active Method Samples Only The active method is the method that is currently running on the Autosampler or the method currently shown on the display A method is a procedure that may consist of a single injection or a series of injections of sev eral samples calibrants or a combination of samples and calibrants The method also defines the location of each sample or calibrant vial as well as the injection volume and the number of replicates for each sample or calibrant The default method method00 provided with the Autosampler is set up to perform a single sample injection This section describes how to make some simple changes to this default method The procedure is shown on the facing page To permanently store changes to the default active method refer to the Creating New Methods section in this chapter Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Active Method Samples Only Creating Methods and Sequences Procedure for Modifying the Default Active Method Samples Only MAIN SCREEN METHOD00 STORED DEFAULT READY E E Pf sea or C erry Press F2 to move to the Method Screen The data field for the fir
58. Screen INJECTION PERFORMANCE PARAMETERS To move between INJECTION PERFORMANCE PARAMETERS data fields ona LOOP MODE partial VENT MODE page use the DELAY TIME 0 AIR CUSHION cursor arrow keys NEEDLE LEVEL 10 EXCESS VOL All data field entries TIME SAVER Off are in lower case SELECT LOOP MODE PARTIAL FIXED TS TT To move to page 2 of the Performance Parameters press F8 MJ To go back to the Instrument Setup Screen press the key SAMPLE PUMP REAGENT MIXING PERFORMANCE PARAMETERS PERFORMANCE PARAMETERS 2 of 3 SAMPLE PUMP REAGENT MIXING SPEED medium SPEED IN slow SYRINGE 250 SPEED OUT fast PAUSE 2 SELECT SAMPLE PUMP SPEED EA Ee eN rast vestf __ wore To move to page 3 of the Performance Parameters Press F8 MJH To move back to page 1 from page 3 press F8 MJH again To go back to the Instrument Setup Screen press the key FLUSH PUMP PERFORMANCE PARAMETERS FLUSH PUMP PERFORMANCE PARAMETERS 30f3 SPEED medium HARD KEY CYCLES 1 VOLUME 700 FLUSH CYCLES PRE 1 POST 1 END OF METHOD CYCLES 0 SELECT FLUSH PUMP SPEED mEn m N S a a a Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix B Changing the Performance Parameters Changing Information on the Screen Changing Information on the Screen INJECTION PERFORMANCE PARAMETERS l l INJECTION PERFORMANCE PARAMETERS Message line provides LOOP MODE partial VENT MODE instructions on information DELAY TIME 0
59. T VIAL NUMBER 1 225 CEN E a u U E E A TIME 0 10 0 10 0 10 carrot To select an item from the list press F1 EN and enter the item number when prompted then press the key The carrot will move to the selected item Alternatively you can use the cursor arrow keys to scroll up and down the list Jo delete an item select the item and press the key Jo permanently save these changes press the key Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences NOTES NOTES Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Sequences Getting Ready A Checklist 5 2 Powering Up the Autosampler 5 3 The Default Top Level Main Screen 5 3 Starting a Method 5 4 Starting the Active Method 5 4 Recalling and Starting a Stored Method 5 5 Starting a Sequence 5 7 Starting a Priority Method or Random Priority Method 5 8 Starting a Priority Method No Active Method or Sequence Running 5 8 Interrupting the Active Method orSequence and Running the Priority Method 5 9 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Sequences This chapter describes how to run Methods
60. TT Stator 7725 010 Stator Screws 3 7125 025 Figure 7 14 Injector Valve Disassembly Note All part numbers in Figure 7 14 are Rheodyne Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Dismantling the Injector Valve pg Reassembling the Injector Valve 1 Lineup the rotor seal as shown in Figure 7 15 The rotor seal slots face the stator 2 Replace the stator ring so that the pin in the 60 stop ring enters the mating hole in the stator ring Notch Rotor pin 60 stop ring Rotor seal slots Figure 7 15 Correct Position of Rotor Seal 3 Loosen the pressure adjusting screw about 1 2 turn Note the original position of the two red dots as shown in Figure 7 16 Red alignment dots Top View of Injector Pressure adjusting screw Figure 7 16 Guides on Adjusting Screw and Body of Injector Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Dismantling the Injector Valve Maintenance 4 Put the stator face assembly on the stator The three pins on the assembly fit into the mating holes in the stator only one way 5 Replacethe stator and stator face assembly on the valve so that the pin in the stator ring enters the mating hole in the stator 6 Add thethree stator screws Tighten each an equal amount until the screws are tight Artisan Technology Group
61. a a jf ver 2 Press F4 to move to the Calibration Screen CALIBRATION SCREEN GTV GO TO VALVE XY CALIB POSITION CXY CALIBRATE X amp Y NDLE GO TO NEEDLE Z CALIB POSITION CZ CALIBRATE NEEDLE Z X HHH Y HHH Z HH C CEA ra r u E E Ee Go to the next page to continue Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Calibrating the Sample Needle Calibrating the Sample Needle continued CALIBRATION SCREEN GTV GO TO VALVE XY CALIB POSITION CXY CALIBRATE X amp Y NDLE GO TO NEEDLE Z CALIB POSITION CZ CALIBRATE NEEDLE Z X HHH Y HH Z HHH C CA nra rc e E Be Ee F1 F2 F3 F4 Fe F7 F8 Press F1 R amp A This positions the needle over the injection valve Push the needle all the way down into the valve Being careful to keep your fingers clear of the Sample Needle Assembly press F4 W This sets the z coordinate for the needle and then raises it up out of the injector valve The new Z value appears in the Calibration Screen Check the calibration by pressing F3 QE This positions the needle over the injection valve and then lowers it all the way into the valve Verify that the upper surface of the vial probe firmly contacts the lower surface of the needle cartridge Record the new value that appears in the Z data field of the Calibration Screen You can overwrite the old Z value w
62. agnostics Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics Read Firmware Part Number This selection from page 3 of the Diagnostics Screen displays the Firmware part number PAGE 3 OF THE DIAGNOSTICS SCREEN OUT OUTPUT RELAYS TEST PART READ FIRMWARE PART NUMBERS CLRM CLEAR ALL METHODS CRAM CLEAR BATTERY RAM BCD _ BCD OUTPUTS TEST COUNT 01 M CS Ce Ee ME TTS Press F2 to display the part numbers The following screen appears CARD PART NUMBER N293 XXXX BOOT FIRMWARE XX 2 Press the key to redisplay page 3 of the Diagnostics Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Running the Diagnostic Tests Clear All Methods This selection from page 3 of the Diagnostics Screen clears all stored methods PAGE 3 OF THE DIAGNOSTICS SCREEN OUT OUTPUT RELAYS TEST PART READ FIRMWARE PART NUMBERS CLRM CLEAR ALL METHODS CRAM CLEAR BATTERY RAM BCD _ BCD OUTPUTS TEST COUNT 01 lout_Jeartfocurmp cramp ecof A Press F3 to start to clear all methods The following screen appears CLRM CLEAR ALL METHODS ARE YOU SURE ECE ECE E E A E E Gas 2 Press F1 to run the test or press F2 IMN to redisplay page 3 of the Diagnostics Screen Artisan Technology Group Quality Instrumentation G
63. allow you to get your system up and running quickly Using the Manual The main function of this manual is to give you all the information you need to operate the Series 200 LC Autosampler as productively as possible using all of the instrument features Caution Theinstrument should be used according to VAN the instructions provided in this manual If used otherwise the protection provided by the instrument may be impaired Note The unpacking and setup procedures for the Series 200 LC Autosampler are included in Chapter 2 of this manual If you have not installed the instrument you should first refer to the instructions given in Chapter 2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preface Using the Manual Definitions Definitions The following definitions of key terms will help you in using this manual to operate the Series 200 LC Autosampler Method A method is a procedure that may consist of a single injection or a series of injections of sev eral samples and or calibrants The method also defines the location of each sample or cali brant vial as well as the injection volume and the number of replicates for each sample calibrant There is a default method method00 provided with the Autosampler You can make changes to this default method and save those changes by saving them under a new method number from 1 19 If you choose not to save the chang
64. alog or toa PerkinElmer Product Description List Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Unpacking Your Series 200 LC Autosampler Installation Removing the Packing Foam 1 Cut the shipping strap securing the needle assembly 2 Hold the needle assembly in the most upright position 3 Slide the packing material forward to remove as shown in Figure 2 1 Hold needle assembly in the most upright position 1 Cut the shipping strap Slide packing material forward to remove Figure 2 1 Removing the Packing Foam 2 7 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Making the Electrical Connections Making the Hectical Connections Caution The Series 200 LC Autosampler is shipped from VAN the factory with The operating voltage set to 120 VAC The main AC power switch set to the OFF position No fuses installed You need to select the appropriate voltage set ting and install the proper fuse s before install ing the line cord and applying AC power Checking Electrical Power Requirements The Series 200 LC Autosampler requires a grounded nominal 100 120 220 230 or 240 VAC source The Series 200 LC Autosampler uses 70 Watts maximum power The AC line voltage must remain within 10 of the nominal specified voltage 6 10 for 240 VAC If the electrical supply voltage
65. ample injection order samples only 4 4 setting up for samples only 4 3 setting up when all method options are enabled 4 5 starting 5 4 Adding method options 3 6 deriv dilut option 3 8 method edit option 3 9 random sampling option 3 10 serial dilution option 3 11 standardize option 3 7 timed events option 3 7 Autosampler components 1 4 configuring 3 1 3 20 cover 7 16 description 1 2 1 3 performance parameters See Configuring the Autosampl er powering up the Autosampler 3 2 5 3 specifications 1 17 system parameters See Defining system parameters B Buffers 2 2 C Calibrants see also Method Standardize Screen adding the standardize option 3 7 Calibration Label 6 4 6 5 Calibration Screen 6 3 accessing 6 3 Column column tubing replacement part numbers 7 31 connecting to the Autosampler 2 15 location of column tubing connection 2 15 replacing the tubing to the column 7 31 scavanger 2 17 Communications accessing the Communications Screen 3 18 BCD port output 3 16 defining the options 3 16 list of options 1 17 pin assignments for BCD cable 3 17 selecting the communication option 3 19 serial ports 3 16 Configuring the Autosampler changing the Default Performance Parameters B 1 B 7 Performance Parameter Screen B 4 reviewing the Default Performance Parameters 3 2 B 1 Corrosion 2 3 solvents which may corrode the Autosampler 2 3 Creating a new method from within the active method 4 19 4 20 from within the directory
66. and Sequences on your Series 200 LC Autosam pler It includes the following information e Getting Ready a Checklist e Powering up the Autosampler e Starting a Method e Starting a Sequence e Starting a Priority Method Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Sequences Getting Ready A Checklist Getting Ready A Checklist Before you start a method or sequence use the following checklist to insure that you are ready to begin running samples 1 Review the safety and warning notes at the beginning of this manual Safety and Regulatory Information 2 Verify that the tubing to your pump column and detector is properly connected 3 Verify that the electrical connections to relays and timed events are properly con nected 4 Start the pump and detector Flush and equilibrate both with the mobile phase required for your analysis conditions To set the pump and detector conditions refer to the user s manual provided with the instruments 5 Start the Autosampler 6 Flush the Autosampler by pressing the key on the keyboard The default number of flush cycles per key press is set at 2 Each cycle flushes 500 uL of sol vent through the system for a total of 1 0 mL which corresponds to the amount of solvent held in the system tubing To change this value refer to Appendix B in this manual Note If you have not run solvent through the Au
67. ange for highlighted field 1 13 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Using the Keyboard and Display Entering Non numeric Information into Data Fields within a Screen Use the To Function Keys If a highlighted data field requires a non numeric entry the options for that field appear as screen defined soft keys The message line also provides information on the options available for the highlighted field To change the current entry in the highlighted field press the corresponding function key F1 through F8 For example in the following Method Options Screen in order to change the OFF entry to ON and thereby enabling the STANDARDIZE option press F 1 MX METHOD OPTIONS SCREEN Message line provides METHOD OPTIONS information on available STANDARDIZE off TIMED EVENTS off options __J DERIV DILUTE off METHOD EDIT off RANDOM SAMPLING off SERIAL DILUTION off SELECT STANDARDIZE OPTION ON OFF Screen defined soft keys gt BKNE es es ee ee ee These change as you move from field to field 1 14 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Using the Keyboard and Display Scrolling Through Sc reen Lists Introduction Use the cursor arrow keys OS If a list of items is displayed on a screen use the cursor arrow keys to move the carrot up a
68. art of Figure 7 5 press and hold the needle cartridge up against the face plate so that the lower surfaces of the two plates are flush While holding the needle cartridge securely in place tighten the the two thumbscrews Switch on the power to the Autosampler Press the key on the keyboard and flush solvent through the system until there are no bubbles in the sample tubing To ensure that there are no bubbles inspect closely the tubing near the point where it enters the union 7 12 9 95 Rev C Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance O Slotted Needle nut Part No N293 1064 Q PEEK nut Part No 0992 0513 Needle spring g ie a E ne Needle cartridge n Sample needle Part No N293 0023 Union Part No 0990 3289 Union holder Part No N293 1081 PEEK nut Sample tubing from sample syringe Sample tubing from sample syringe must go under the loop in the sample needle Needle cartridge face plate Needle cartridge thumbscrews Lower surfaces of the needle cartridge and face plate should be flush Figure 7 5 Removing Replacing the Sample Needle 9 95 Rev C 7 13 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Notes Notes 7 14 Artisan Technology Group Quality Instrumentation Guaranteed
69. artisantg com Quality Control Good Laboratory Practices Safety and Regulatory Information Quality Control Good Laboratory Practices Quality Contol The user should develop appropriate quality control procedures for the LC Autosampler and the entire LC system to ensure suitability for its intended use These procedures typically consist of periodic performance verifications and routine inspections and suitability tests Certificate of System Conformity Each Series 200 LC Autosampler is carefully built and tested in a controlled system in accor dance with the requirements specified in its applicable PerkinE Imer Final Assembly and Test Specification Each instrument is certified to meet its functional and performance specification upon release to shipment The integrity of this quality system is routinely audited and is certified by the British Standards Institution as meeting all requirements of I SO 9001 1987 Certifi cation No FM 22179 Instrument Performance Venrific ation IPV To maintain functional performance PerkinE Imer recommends a yearly Instrument Perfor mance Verification IPV of the Series 200 Autosampler by a PerkinElmer Service Engineer to ensure its operation within published specifications These tests consist of measuring the most important autosampler characteristics such as Relative Standard Deviation of peak area and sample carryover Certification is available for regulatory compliance Contact your loca
70. ation and Diag nos tics Runn ing the Diag no stic Tests Flash Test This test calculates a checksum for the flash memory parts which is then compared with a checksum stored on the flash memory If a failure occurs call your PerkinElmer Service Engineer PAGE 1 OF THE DIAGNOSTICS SCREEN KPD1 KEYPAD DISPLAY TEST 1 KPD2 KEYPAD DISPLAY TEST 2 FLSH FLASH TEST RAM RAM TEST BBUP _ BATTERY BACKUP TEST COUNT 01 E kroz rush Pram Peeve A Press F3 to start the test If the test is successful the following screen appears FLSH FLASH TEST PASS 01 FAIL 00 2 If a failure occurs a screen similar to the following appears FLSH FLASH TEST U14 U15 CHECKSUM IS XXXX CORRECT CHECKSUM IS XXXX PASS 00 FAIL 01 Press the key to redisplay page 1 of the Diagnostics Screen 6 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics RAM Test This diagnostic writes a number of test patterns into RAM and then reads them to verify that they were written correctly Each RAM is tested with three patterns The first is mod ule 256 of each byte address The second is the hexadecimal value 55 and AA written into the same part of every other byte The system RAM is tested first then the battery backup RAM is tested The contents of the battery backup RAM are stored in the system during testing The battery
71. backup RAM will not be tested if the system RAM fails If a failure occurs call your PerkinElmer Service Engineer PAGE 1 OF THE DIAGNOSTICS SCREEN KPD1 KEYPAD DISPLAY TEST 1 KPD2 KEYPAD DISPLAY TEST 2 FLSH FLASH TEST RAM RAM TEST BBUP _ BATTERY BACKUP TEST COUNT 01 E xeo2 rush Pram Peeve _ ore Press F4 ZNE to start the test If the test is successful the following screen appears RAM TEST PASS 01 FAIL 00 2 If a failure occurs the following screen appears RAM RAM TEST RAM U12 OR U13 FAILS TESTS AT ADDRESS XXXX DATA WRITTEN IS XXXX DATA READ IS XX Press the key to redisplay page 1 of the Diagnostics Screen 6 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Running the Diagnostic Tests Battery Bac kup Test Caution This test will delete all methods the sequence A configuration and calibration parameters This test is designed to test the battery backup RAM The contents for the battery backup RAM are stored in the system during testing The battery backup RAM will not be tested if the system RAM fails If a failure occurs call your PerkinElmer Service Engineer PAGE 1 OF THE DIAGNOSTICS SCREEN KPD1 KEYPAD DISPLAY TEST 1 KPD2 KEYPAD DISPLAY TEST 2 FLSH FLASH TEST RAM RAM TEST BBUP _ BATTERY BACKUP TEST COUNT 01 KPD1 aA JFLsH PRAM BBuP a U MORE
72. bes nuts and ferrules from Table 7 5 Table 7 5 Replacement Tubing Nuts and Ferrules Replacement Part Stainless Steel Part No Bio Applications Part No Tubing setions sections 0271 1441 20 cm 0271 1441 20cm 1 16 in O D in O D N930 2678 N930 2678 5 ft 1 16 in O D ft 1 16 in O D 0 007 in 1 D 0 007 in 1 D N293 1089 30 cm 1 16 in O D N930 2650 5 ft 1 16 in O D 0 007 in D 0 010 in 1 D 0089 1480 50 cm 1 16 in O D 0 007 in D Rheodyne short nut 0990 4956 N930 2676 Pkg of 5 Rheflex Rheodyne long nut 0990 4974 Nuts and Ferrules Rheodyne ferrule 0990 4947 3 Using a standard 1 4 wrench remove the nut and ferrule connecting the pump tube to port 2 4 Slidethe nut and ferrule over the tube Orient the nut and ferrule as shown in Figure 7 22 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Replacing the Tubing to the Pump 5 Insert the tube into port 2 on the bottom of the injector value Push the tubing into the port until it stops Finger tighten the nut as far as it will go Take the 1 4 wrench and tighten the nut an additional 1 2 turn 6 Completely remove the nut and ferrule The leading edge of the tube should be tapered towards the tubing There should also be about 1 8 inch 3 mm of tubing that protrudes beyond the ferrule 7 Add the nut and ferrule to the other end of the tub
73. ce tighten the the two thumbscrews 10 Locate the sample tubing coming from the sample syringe Slide the sample tubing under the curved top portion of the sample needle as indicated in the lower portion of Figure 2 10 11 Slide the remaining PEEK nut over the end of the sample tubing and thread it into the bottom of the union 12 Secure the sample tubing to the needle cartridge using the clamp and screw 2 18 9 95 Rev C Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making the External Tubing Connections Installation Slotted needle nut p m a Curved top portion of the sample needle Needle spring 0 PEEK nut Needle cartridge gt y PEEK nut A vA lt _ _ __ Clamp and i screw D Sample tubing from sample syringe Needle cartridge face plate PEEK nut Lower surfaces Needle cartridge of the needle thumbscrews cartridge and face plate should be flush Clamp and screw Figure 2 10 Connecting the Sample Needle 9 95 Rev C 2 19 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Making the External Tubing Connections 5 Connecting the Hush Syringe Tubing to the Solvent Container 1 Refer to Figure 2 11 Locate the flush line tubing with the solvent filter Part No 0990 3615 attached to the free end The
74. ce and Information 1 800 762 4000 Supplies accessories and replacement parts can be ordered directly from PerkinElmer using the eight digit part numbers provided in this manual PerkinEImer s catalog service offers a full selection of high quality intrumentation reagents and consumables through the HPLC and CE Supplies Catalog For information to place an order or request a free catalog If you are located within the U S call toll free 1 800 762 400 8 30 a m to 7 p m EST Your order will be shipped promptly usually within 24 hours If you are located outside the U S call your PerkinElmer Sales Representative Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preface Using the Manual Customer Service Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Intoduc ton A Description of the Autosampler 1 2 Series 200 LC Autosampler Features and Benefits 1 3 Main Components of the Series 200 LC Autosampler 1 4 Keyboard and Display 1 4 Input Output Connector Panel 1 4 Sample Tray 1 4 Sample Needle Assembly 1 4 Dual Syringe System 1 6 RearPanel 1 7 Using the Keyboard and Display 1 8 Summary of the Keyboard Functions 1 9 Keyboard Operations 1 11 Instrument Specifications 1 17 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com
75. connecting the flush syringe tubing tothe sol vent container 2 20 description 1 6 location 1 6 7 9 Artisan Technology Group Quality Instrumentation Function keys description 1 8 1 9 1 12 Fuses changing a single fuse 7 6 changing two fuses 7 7 dual fuse configuration 2 11 location 2 10 7 5 part numbers for dual fuse configuration 2 11 7 7 part numbers for single fuse configuration 2 10 7 6 replacing 7 5 single fuse configuration 2 10 Injection range 1 17 Injector valve cover 7 20 dismantling 7 24 reassembling 7 26 reinstalling in the Autosampler 7 28 removing from the Autosampler 7 20 7 23 rotor seal 7 24 stator face assembly 7 24 valve actuator arm 7 22 Injector valve port location 1 5 2 18 Input Output Connector Panel connecting to LC Series 200 Pump 2 22 2 24 connecting to Turbochrome 2 22 2 24 description 1 4 2 21 making connections 2 21 2 24 Instrument log viewing 6 26 6 27 7 4 sopropanol removal of air bubbles 7 9 K K eyboard description 1 3 1 4 1 8 entering non numeric information 1 14 entering numeric information 1 13 operations 1 11 responding to screen prompts 1 16 scrolling through screen lists 1 15 selecting data fields 1 13 selecting screen options 1 12 summary of functions 1 9 Guaranteed 888 88 SOURCE www artisantg com Line cord description 2 12 installation 2 12 linearity 1 17 Loop mode fixed D 1 Maintenance Preventative 7 3 Routine 7 4 Main Screen defined
76. ctor Card Orientation for 120 Volt Operation AOS Pa j a yd oas Voltage Selector sa Rt amans SR Card a ne _ g Voltage Selector Power Input Arrow Module Cover a gt 100 Voltage Selector Arrow Voltage Selector Card Orientation for 220 Volt Operation Figure 2 2 Power Input Module exploded view 4 Position the number 100 120 220 240 on the side of the voltage selector card so that it is opposite the voltage selector arrow THIS NUMBER MUST CORRESPOND TO THE VOLTAGE FOR YOUR LOCATION Figure 2 2 shows the voltage selector card settings for both the 120 V and the 220 V position Reinstall the voltage selector card into the power input module Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Making the Electrical Connections Installing the Primary Fuse s After setting the voltage range select and install the proper fuse s The fuse block is located on the power input module cover See Figure 2 3 for the location of the fuse block Single Fuse Configuration 1 Select the required fuse from Table 2 5 below Table 2 5 Single Fuse Configuration Table Use only 3AG 1 4 x 1 1 4 250 V Fuses Voltage Selector Insert the following fuse 1 6 amp Slow Blow 0998 1613 1 6 amp Slow Blow 0998 1613 0 8 amp Slow Blow 0999 1671 0 8 amp Slow Blow 0999 1671 2 Slide the fuse into the fuse holder See Figure 2 3
77. e F4 This chapter describes how to perform routine and preventive maintenance procedures on your Series 200 LC Autosampler It includes the following information e Guidelines for Maintaining the Autosampler e Replacing a Fuse e Changing the Sample and Flush Pump Syringes e Replacing the Sample Needle e Changing the Transfer Tubing e Removing the Injector Valve e Dismantling the Injector Valve e Changing the Sample Loop e Replacing the Tubing to the Column e Replacing the Tubing to the Pump e Replacing the Waste Tubes Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Notes Notes Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Guidelines for Maintaining the Autosampler Maintenance Guidelines for Maintaining the Autosampler The Autosampler is constructed with high quality components and requires little mainte nance other than to keep it clean The following guidelines are offered to keep your Autosampler up and running e Wipe up spilled liquids immediately with a soft lint free cloth or absorbant paper to pre vent damage to the instrument e Carry out the maintenance tasks described in this chapter e If you have any problems or inquires contact your nearest PerkinElmer office e If you need to return the instrument for maintenance send it in the original packing Preventative Measures to Av
78. e Main Screen appears COPYRIGHT AND SELF TEST SCREEN SERIES 200 LC AUTOSAMPLER COPYRIGHT C PERKIN ELMER 1995 SOFTWARE REVISION 1 00 RUNNING SELF TEST DEFAULT MAIN SCREEN METHOD00 STORED DEFAULT E C0 ee Ee eS a The Default Top Level Main Screen The Main Screen provides access to all of the Autosampler s functions The soft keys dis played along the bottom of the default Main Screen correspond to the functions that are available when you first power up the Autosampler Later in this chapter you will see how to add other optional functions to the Main Screen Reviewing the Default Performance Parameters Table 3 1 lists the default sample injection and flush pump parameters for the Autosampler These parameters have been set toinsure high precision low carryover high throughput and economical use of sample If you need to change any of these settings due to a special requirement of your analysis you can do so from the Default Main Screen as described in Appendix B Changing the Performance Parameters Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Reviewing the Default Performance Parameters Configuring the Autosampler Table 3 1 Default Performance Parameters ES Default Acceptable Parameter Description Value or Range 1 Air Cushion Volume of air drawn up before sample 10 uL 1 99uL Air cushion is used to prevent mixing of the flush buffer and
79. e Method Screen and saves the changes until you turn the power off on the Autosampler When you restore power the default values for the method will also be restored To permanently store the changes refer to the Creating New Methods section in this chapter To make changes to a method containing timed events press F5 from the Method Screen and use the cursor arrow keys to scroll through the list of events To turn off T E 1 or T E 2 use the cursor arrow to move to T E 1 or T E 2 and press the 0 numeric key The YES will be deleted from the field To change the time of an event select the event and press the F3 key Then use the numeric keys to enter a new time To delete an event select the event and press the key To add an event use the cursor arrow keys to scroll down to the location in the event list where you want to add the event and press the key If there is a time slot available between the two successive events an event will be added immediately after the first event otherwise the system will ignore the key For example if the time for event 5 is 0 30 and the time for event 6 is 0 31 you will not be able to add an event between 5 and 6 The time difference between the two events must be at least 0 02 minutes Save the changes as described in step 6 To permanently store the changes refer to the Creating New Methods section in this chap ter 4 10 Artisan Technology Group Qualit
80. e RDY IN connec tor If a failure occurs call your PerkinElmer Service Engineer PAGE 2 OF THE DIAGNOSTICS SCREEN PELT PELTIER COMMUNICATIONS TEST DUA1 DUART TEST INTERNAL DUA2 DUART TEST EXTERNAL INP REMOTE INPUTS TEST COUNT 01 CE CONE COS TT Press F4 ENJ to start the test The following appears during the test When the Ready n Loopback connector is plugged into the RDY IN connector the 0 in the above screen should change to 1 RDY INJ i IN IN Co JE Tet STRT IC TE2 ON OFF Figure 63 Location of the RDY IN Connector 6 19 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Running the Diagnostic Tests Output Relays Test This test is designed to test the output relays If a failure occurs call your PerkinElmer Service Engineer PAGE 3 OF THE DIAGNOSTICS SCREEN OUT OUTPUT RELAYS TEST PART READ FIRMWARE PART NUMBERS CLRM CLEAR ALL METHODS CRAM CLEAR BATTERY RAM BCD _ BCD OUTPUTS TEST COUNT 01 lout_Jeartfourmfcramp ecof A Press F1 to start the test The following screen appears during the test Monitor the status of the output relay directly with a DVM START OFF INJ1 INJ2 OFF TE1 OFF TE2 OFF CESI ECEN _ Gs 2 Press F2 to switch the relay on or off Press F1 to select the next relay Press the key to redisplay page 3 of the Di
81. e TANK is dispensed first Sample from the vial beginning the 2nd set in this example 1 is dispensed next VOLUME Volume of diluent 1st set or sample 2nd set MIX CYCLES Number of times the mixture in the target vials is mixed For best results set this value to 2 as shown in the screen above EQUIL TIME The amount of time mixture in target vial is allowed to equilibrate between vial to vial transfers of liquid 9 95 Rev C 4 15 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining the Active Method All Options Enabled Example 2 Dilution Refer to Figure 4 2 Thedilution tray is set up so that the first 20 vial positions are reserved for sample source vials the second 20 vial positions 21 40 for internal standards and the third set of 20 vial positions 41 60 for target vials The screen entries and the proce dure for this dilution scheme are on the facing page 100 uL of diluent is transferred from the Diluent Tank to target vial 41 50 uL of sample in sample vial 1 is transferred to target vial 41 25 uL of internal standard is transferred from vial 21 to target vial 41 For the second dilution 100 uL of diluent is transferred from the Diluent Tank to target vial 42 fol lowed by 50 uL of sample from sample vial 2 Then 25 uL of internal standard is tranferred from vial 22 to target vial 42 This process will c
82. e should also be about 1 8 inch 3 mm of tubing that protrudes beyond the ferrule 7 Add the nut and ferrule to the other end of the tubing the end going to the column Use nuts and ferrules recommended by the column manufacturer or if you are inserting an in line device between the Autosampler and the column use nuts and ferrules compati ble with the device 8 Rethread the nut and ferrule going to the injector into port 3 on the injector valve Fin ger tighten the nut as far as it will go Take the 1 4 wrench and tighten the nut an addi tional 1 8 turn 9 Donot connect the other end tothe column or in line device until after you reinstall the injector valve Nut Ferrule Tubing amp Figure 7 20 Correct Orientation of Nut and Ferrule on Tubing Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Replacing the Tubing to the Pump Maintenance Replacing the Tubing to the Pump If you have not already done so remove the injector valve from the Autosampler as described in Removing the Injector Valve section in this chapter 1 Refer to Figure 7 21 which shows the location of all the ports on the bottom of the injec tor valve and their connections Sample loop connected P t2 UMP por Na toports 1 and 4 Waste vent port 6 Column port 3 Waste vent port 5 Figure 7 21 Bottom View of the Injector Valve 2 Select the appropriate replacement tu
83. ed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics This chapter contains procedures for calibrating and running diagnostics on your Series 200 LC Autosampler It includes the following information e Calibrating the Sample Needle e Accessing the Diagnostics Screen e Running the Diagnostics Tests Keypad Display Tests FLASH Test RAM Test Battery Backup Test Peltier Communications Test DUART Test Internal DUART Test External Remote Input Test Output Relays Test Read Firmware Part Number Clear All Methods Clear Battery RAM BCD Outputs Test e Viewing the Instrument Log Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Notes Notes Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibrating the Sample Needle Calibration and Diagnostics Calibrating the Sample Needle You need to run the following calibration procedure whenever you change the type of injec tor MAIN SCREEN READY E C SB A a From the Main Screen press F7 to move to the Configure Screen CONFIGURE SCREEN CONFIGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION LOG INSTRUMENT LOG inst commipiac Tcat_Jtoc
84. eedle to the Injection Valve Port e The needle is lowered into the port and the sample is injected onto the column e The transport mechanism moves the needle to the flush port and a flush cycle is per formed The sampling modes and the sampling volumes available on the Autosampler are described in detail in the section Reviewing the Default Performance Parameters in Chapter 3 of this manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Main Components of the Series 200 LC Autosampler Introduction Sample Compartment Door Flush Port Injector Valve Port Sample Sample Needle Tray Assembly Injection Valve area MRON On Off Power Switch Keyboard and Display Input Output Connector Panel a Sample Needle Figure 1 2 Front Right Side View of Autosampler Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Main Components of the Series 200 LC Autosampler Dual Syringe System Sample Syringe Flush Syringe Figure 1 3 Front Left Side View of the Autosampler showin
85. een The method number for SET 1 is highlighted SEQUENCE SET METHOD INJECTIONS 3 UTM 6 2 5 UTM ASSAY 3 ENTER METHOD NUMBER 0 19 ser PE Toes Press F6 to link all of the methods in the sequence to the method in SET 1 The Main Screen is redisplayed with the name of the method in SET 1 METHOD03 STORED LINKED UTM READY E CS SB Gd To start the sequence press the key on the keyboard The Main Screen then dis plays the status information for the method in SET 1 of the sequence METHOD03 STORED LINKED UTM SET 1 SAMPLE VIAL 1 REP 1 OF 2 PIPETTING SAMPLE E u Ee a Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Sequences Starting a Priority Method or Random Priority Method Starting a Priority Method or Random Priority Method This section describes how to start a priority method Note that the operations and the screens are identical for starting a random priority method Starting a Priority Method No Active Method or Sequence Running You can start the priority method from any screen by pressing the key on the keyboard As soon as you start the priority method the Main Screen appears and displays information about the status of the priority method similar to the screen shown here PRIORITY SAMPLE VIAL 1 REP 1 OF 2 PIPETTING SAMPLE E CS ee Ee Ee eS a Note if the priority method you defined and the sample tray a
86. een 1 12 description 1 8 Diagnostic Screen 6 6 difference between screens and pages P 2 enabling the screen saver 3 12 entering non numeric information 1 14 entering numeric information 1 13 Error Recovery Screen 3 15 hierarchy 1 8 1 10 Main Screen P 2 1 8 1 12 Main Screen on power up 3 2 5 3 Method Derivatization Screen 4 11 Method Dilution Screen 4 15 4 17 Method Edit Screen 4 24 Method Screen 1 13 4 3 Artisan Technology Group Quality Instrumentation Method Standardize Screen 4 6 Performance Parameter Screen B 4 Priority Method Screen 4 31 Random Priority Screen 4 32 responding to prompts 1 16 saving changes 1 16 scrolling through lists 1 15 selecting options 1 12 Sequence Screen 4 28 Serial Dilution Screen C 3 setting screen contrast 3 12 System Clock Screen 3 13 Tray Type Screen 3 14 Sequence defined P 3 interrupting with a priority method 5 9 rules for defining a sequence 4 26 setting up a sequence 4 28 starting 5 7 Sequence Screen 4 28 Serial dilution adding the serial dilution option 3 11 example C 2 Serial Dilution Screen C 3 setting up the Serial Dilution Screen C 3 Serial Dilution Screen C 3 summary of data field requirements and ranges C 6 Shipping container 2 6 items included 2 6 Solvent filter replacing 7 16 7 17 Solvents 2 2 auto ignition temperature below 100 C 2 4 Solvents with Low Boiling Points 2 2 Specifications instrument 1 17 Starting a priority method 5 8 Starting a sequence 5 7 Star
87. em a Pump Check short term pump flow stabil reproducibility ity e Good retention time e Poor reproducibility Pump Note Retention performance on the pump should be 0 1 RSD or better f to eliminate the pump as a source of e Poor retention time problems Refer to pump manufac turer s guide Column Refer to column manufacturer s guide Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Troubleshooting Table 1 Performance Problems Flush Solenoid Sample T Flush Line Tubing Solvent Filter gt J Figure81 Dual Syringe System Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Table Performance Problems Troubleshooting Y connector To Column Bottom view of the injector valve Figure 8 2 Location of Waste Lines on Injector Valve Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Troubleshooting Table 2 General Instrument Problems Troubleshooting Table 2 General Instrument Prob lems General Insi ument Possible Cause s Check and or remedy Problems Autosampler wi
88. er share many common operating features that will allow you to get your system up and running quickly The Series 200 LC Autosampler provides you with interchangeable technol ogy so that you do not have to buy a new Autosampler and learn new user interfaces every time your needs change Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Series 200 LC Autosampler Features and Benefits Introduction Series 200 LC Autosampler Features and Benefits e Upgradable to biocompatible versions for maximum flexibility e Small compact design 12 5 inches 31 8 cm wide saves valuable bench space e Directory of up to 20 methods e Ability tolink up to 10 methods together in a sequence e Priority method which allows you to interrupt a method or sequence to run critical sam ples The method or sequence resumes once the priority samples are analyzed e Edit the active method or sequence in real time with easy access function keys e Large backlit liquid crystal display allows clear viewing of autosampler method and operating parameters at all times e Tactile keyboard with unique key click so that you know your entry has been made e Phonetype contact closure connections and finger tight plumbing fittings make it simple to connect or disconnect your system e Full method documentation including modification with method name number date and time stamp e Visual access front door pa
89. es 200 LC Autosampler Terminal Connectors Table 2 9 Input Output Terminal Connectors INJ1 This contact closure activates for a duration of 2 seconds when the injection value rotates from load to inject This is typically used to start an external device typically a pump INJ 2 Identical to INJ 1 except this is typically used to start a data handling system START This contact closure activates at the beginning of the sam pling operation and is typically used to autozero the detec tor READY IN This contact closure is used when the Autosampler is con Required for operation trolled by an external device typically a pump The Autosampler waits to start sampling until this contact closes thereby synchronizing the timing between the Autosampler and the controlling external device TElandTE2 These contact closures actuate at a time specified in the Method and control an external device When activated the TE1 and TE2 contacts are switched and held for 2 seconds Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Making Input Output Terminal Connections Making a Connection between Modular Cable and Twisted Pair Wires You will need to make this connection when you are connecting the Autosampler to accesso ries likethe LC Series 200 Pump the LC 135 235C Diode Array Detector or Turbochrom The proper way to connect the modular cable and the two wires from one
90. es in this way the changes will be lost when you turn the power off on the Autosampler Screens and Pages Screens display the information for defining the operation or configuration of the Series 200 LC Autosampler If all of the information for a particular operation set cannot be displayed on a single screen then the screen is divided into pages Main Screen The Main Screen is the top level screen that provides you with access to all of the Autosam pler s functions All of the functions are displayed along the bottom of the screen The Main Screen has two display formats The first format shown here is the one displayed when the Autosampler is idle METHOD00 STORED DEFAULT E C0 ee Ee Be eS a The second format is displayed when the Autosampler is performing an operation It pro vides status information about the operation A typical example of status information is shown here for an active methodO0 currently being run METHOD00 CHANGED DEFAULT SAMPLE VIAL 1 REP 1 OF 2 PIPETTING SAMPLE E CS ee E Be a Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Definitions Preface Using the Manual Active Method The active method is the method that is currently running on the Autosampler or the method currently shown on the display Sequence A sequence is a series of stored methods that are linked together When you start a sequence each method in the seque
91. esults set this value to 2 as shown in the Serial Dilution Screen at the top of this page Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix D Fixed Loop Mode This appendix contains the recommended sample loop and sample syringe sizes when oper ating the Series 200 LC Autosampler using the fixed loop mode In the fixed loop mode the volume of sample injected onto the column is defined by the size of the sample loop Since the volume of the sample loop is exact to within very close toler ances this mode is suitable for applications requiring high precision In the fixed loop mode the autosampler withdraws 10 times the amount specified in the injection VOLUME field on the method screen The loop is rinsed with the sample and the excess is flushed to waste A typical method screen is shown in Figure D 1 In this method the injection volumeis 5 ul Therefore 50 uL of sample will actually be withdrawn from vial 1 and rinsed through the loop METHOD SCREEN METHOD00 CHANGED FIRST LAST VOLUME REPLICATES TIME 1 1 5 1 10 ENTER INJECTION VOLUME 0 1 2500 uL M ee Sd ee ee Figure D 1 Method Screen Make sure that the injection VOLUME corresponds as closely as possible to the size of the sample loop This ensures that the system is well flushed with sample and the sample loop is overfilled so that the sample concentration will be correct To ensure adequate e
92. ethod Derivatization Screen with some typical entries METHODOO STORED DERIV SAMPL VOL 400 VIAL 1 2 VOLUME 100 50 MIX CYCLES 0 5 REACT TIME o 1 ENTER SAMPLE VOLUME 0 2500uL reap vat Pvoufmx Prmef 7 eee Ee To save these entries press the key This also takes you back to the Method Screen and saves the changes until you turn the power off on the Autosampler When you restore power the default values for the method will also be restored To permanently store the changes refer to the Creating New Methods section in this chapter To modify the entries press F4 from the Method Screen A screen similar to the one in step 7 will appear with the Sampl Vol field highlighted You can use cursor arrow keys to move to the appropiate field or press the corresponding function key Save the changes as described in step 7 To permanently store the changes refer to the Creating New Methods section in this chap 4 12 9 95 Rev C Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Active Method All Options Enabled Creating Methods and Sequences Setting Up a Dilution In order to have access to the function from the Method Screen you need to make sure that you have inserted the dilution tray Part No N293 0314 into the Autosampler NOTE To achieve the best performance use the default Performance Parameter settings To review or change the Pe
93. etting 500 uL of Diluent and 100 uL of Sample 4 14 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Active Method All Options Enabled Creating Methods and Sequences Procedure and Screen Entries for Example 1 From the Main Screen press F2 to move to the Method Screen From the Method Screen press F1 REM to create a new active method Fill in the fields with the entries shown here Note that the VOLUME specified in this screen refers to the portion of the final dilution in the target vial that is injected METHOD0O0 CHANGED DEFAULT FIRST LAST VOLUME REPLICATES TIME 41 80 20 1 1 0 ENTER FIRST VIAL NUMBER 1 225 new E sro ow Pre E A 2 Press F4 to move to the Method Dilution Screen METHODOO ENTER THE NUMBER OF DILUENT SETS 0 6 0 Enter 2 for the number of DILUENTS and press the key The following screen METHOD00 CHANGED DILUT SAMPL VOL FIRST VIAL TANK 1 VOLUME 500 100 MIX CYCLES 0 2 EQUIL TIME 0 0 ENTER SAMPLE VOLUME 0 0 2500uL Cd BS Gd Ee Se Fill in the fields with the entries shown in the above screen As you move to and highlight each field a description of the information required for that field is displayed in the message line The information required for each data field is summarized here SAMPL VOL The volume of sample in each of the target vials in this example 0 FIRST VIAL First vial ofa source set Diluent from th
94. f ons oar roe Park oer 2 Press F1 to move to the Instrument Setup Screen INSTRUMENT SETUP SCREEN OPT METHOD OPTIONS SCRN SCREEN CONTRAST SAVER PERF PERFORMANCE PARAMETERS CLK SYSTEM CLOCK TRAY TRAY TYPE RECV ERROR RECOVERY fort _fsornJrerr four rravpreov Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Defining System Parameters Adding Method Options The Method Options Screen allows you to customize the Series 200 LC Autosampler so that only those operations which you commonly perform are presented as selections in the Autosampler screens You may find it convenient to use the Autosampler s default configu ration if you inject primarily samples and do not vary their injection volumes If you need to use calibrants or perform dilutions or derivatizations the steps in this section show you how to add those options Note that the options you add here apply to all methods including the Priority Method 4 From the Instrument Setup Screen press F1 to move to the Method Options Screen The field for the STANDARDIZE option is highlighted METHOD OPTIONS SCREEN METHOD OPTIONS STANDARDIZE TIMED EVENTS off DERIV DILUTE METHOD EDIT off RANDOM SAMPLING SERIAL DILUTION SELECT STANDARDIZE OPTION ON OFF Screen defined soft keys b gt EME ae ee ee es es ee These change as you move from field to field Message li
95. fer Tubing section in this chapter pA Disconnecting the Extemal Tubing Connections 1 Disconnect the Autosampler from the pump and the column The pump and column tubes exit the top cutout on the left side of the Autosampler and are marked as shown in Figure 7 10 FROM PUMP amp TO COLUMN J FLUSH DRAIN Jp Fa co vw ya i ca cs Z Ja Mg Lf Figure 7 10 Pump and Column Connections to the Autosampler 2 Open the front access door on the Autosampler and pull the drain tubes extending from ports 5 and 6 on theinjector valve out of the Y drain connector as shown in Figure 7 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Removing the Injector Valve Maintenance Y connector To Column Bottom view of the injector valve Figure 7 11 Removing the Drain Tubes from the Waste Drain Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Removing the Injector Valve Removing the Injector Valve Assembly 1 Refer to Figure 7 12 Remove the stainless steel needle guide Part No N293 1054 Store in a safe place 2 Using the allen wrench supplied loosen the two allen screws on the valve actuator arm Lift the valve actuator arm straight up and off the injector valve stem Valve actuator arm One of two
96. for the recommended sample loop and sample syringe sizes Appendix B provides a list of all the perfor mance parameters and describes how to change them 1 Refer to Figure 7 18 which shows the location of all the ports on the bottom of the injec tor valve and their connections Sample loop connected to ports 1 and 4 Pump port 2 Waste vent port 6 Column port 3 Waste vent port 5 Figure 7 18 Bottom View of the Injector Valve Using a standard 1 4 wrench remove the sample loop attached to ports 1 and 4 Select the appropriate replacement sample loop nuts and ferrules For sample loop types and their part numbers refer to the PerkinElmer HPLC and CE Supplies Catalog or toa PerkinE Imer Product Description List 4 Thread each nut and ferrule into ports 1 and 4 Finger tighten each nut as far as it will go Take the 1 4 wrench and tighten the nut an additional 1 8 turn Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Replacing the Tubing to the Column Maintenance Replacing the Tubing to the Column If you have not already done so remove the injector valve from the Autosampler as described in Removing the Injector Valve section in this chapter 1 Refer to Figure 7 19 which shows the location of all the ports on the bottom of the injec tor valve and their connections Sample loop connected to ports 1 and 4 Pump port 2 ae Waste vent
97. g the Dual Syringe System Dual Syringe System This system shown in Figure 1 3 contains the Sample syringe and the Flush syringe The Sample syringe actually draws up the sample through the sample needle into the sample loop and ejects it into the injection valve port The Flush syringe flushes the sample loop needle and sample tubing between injections Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Main Components of the Series 200 LC Autosampler Introduction Serial Ports A B Power Input Module Fan DO NOT BLOCK Figure 1 4 Rear Panel of the Autosampler Rear Panel The rear panel contains the Power Input Module and the communication ports for the Autosampler The power module accepts the AC line cord and contains the system fuses and voltage selector card The function and procedures required to set up the various communi cation ports are described in the Defining the Communications Options section in Chap ter 3 of this manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Using the Keyboard and Display Using the Keyboard and Display The Autosampler keyboard and Liquid Crystal Display LCD are located on the front panel of the instrument as shown in Figure 1 2 A closer view of the keyboard and display is pro vided herein Figure 1 5 You configure the Autosample
98. ght Check for leaks in the Dual Syringe Reproducibility System refer to Figure 8 1 specifi Retention Time Good cally Check connection between flush line tubing and flush sole noid Check connection between sam ple line and the Sample T Check connections to needle Bubbles forming in flush pump Degass the flush solvent Slow down the flush pump speed Check inlet solvent filter Bubbles trapped in flush syringe Flush the system with isopro or transfer tubing panol Remove the syringe and flush it manually with isopropanol Improperly degassed solvent Use only degassed and filtered HPLC grade solvents for flushing solution and mobile phase Blocked sample needle Remove needle clean flush and check stream If necessary replace needle as described in Chapter 7 of this manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Table 1 Performance Problems Troubleshooting Performance Possible Cause s Check and or remedy Poor Area and Height Incorrect sample syringe size Make sure sample syringe size and Reproducibility sample volume requirements are compatible for example do not use PERT E Og a large syringe for injecting small sample volumes Injector valve waste lines par Check the waste vent lines 5 and 6 tially plugged or injector valve for reduced flow when doing an needs to be rebuilt injection or check to
99. he corresponding function key For example press F7 to select and open the Configure Screen CONFIGURE SCREEN CONFIGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION Press function key F1 through F5 LOG INSTRUMENT LOG to select one of the configuration El GEE tions P ing F8 rest th configuration defauts ss F4_ F2 Fe F4 Fs Configuration Options 1 12 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Using the Keyboard and Display Introduction Selecting Data Fields and Entering Numeric Information Use the cursor arrow keys S and the numeric keys To accept the entry in a highlighted data field and move to the next field use the cursor arrow keys For example when you first enter the top level M ethod Screen shown below the data field for the FIRST sample vial is highlighted To accept the current entry and move to and highlight the LAST sample vial press the right cursor arrow key To change a numeric entry use the numeric keys on the keypad The acceptable range for each highlighted data field is displayed in the message line METHOD SCREEN METHOD STORED ese lis Gllsbralraw Keys to FIRST LAST VOLUME REPLICATES TIME accept the entry and move to 10 1 10 the next field Message line ENTER FIRST VIAL NUMBER 1 225 Defines the acceptable CET E a a a ia r
100. her information Consulter les documents d accompagnement This symbol indicates an operation or condi shock if precautions are not followed due to the presence of high voltage Attention Risque de choc lectrique This symbol indicates the danger of electric Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Safety and Regulatory Information Symbols used on the Instrument Additional graphic symbols used on the instrument are the following Indicates alternating current Indicates the primary protective grounding terminal Indicates the off position of the main power switch Indicates the on position of the main power switch Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Warnings on the Instrument Safety and Regulatory Information Wamings on the Instrument The following warning labels are affixed to the rear panel of the instrument for all models as shown in the figure below FOR PROTECTION AGAINST FIRE HAZARD REPLACE ONLY WITH SAME TYPE AND RATING OF FUSE AFIN D ASSURER LA PROTECTION CONTRE LES RISQUES D INCE NDIE REMPLACER UNIQUEMENT PAR UN FUSIBLE DE M ME TYPE ET DE M ME COURANT NOMINAL DISCONNECT SUPPLY CORD BEFORE OPENING GROUNDING CIRCUIT CONTINUITY IS VITAL FOR SAFE OPERATION OF EQUIPMENT NEVER OPER ATE EQUIPMENT WITH GROUNDING CONDUCTOR DISCONNECTED
101. hich appears on the Calibration Label on the electronic cover in the Autosampler The location of the Calibration Label is shown in Figure 6 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibrating the Sample Needle Calibration and Diagnostics F Calibration Label PERKIN ELMER Serial No 293N Y Z To reduce the chance of electrical shock do not remove this cover No user serviceable parts are inside Refer servicing to Perkin Elmer qualified service personnel X A This equipment must be de energized for 60 seconds to allow safe discharge of capacitors before servicing Internal Fuses Power Supply Board Fuse Circuit Fuse Type 250V Rated Only F1 5V 3 0A 3AG SLOW BLOW F2 12V 1 0A 3AG SLOW BLOW F3 24V 5 0A 3AG SLOW BLOW For protection against fire hazard replace only with same type and rating of fuse Figure 6 1 Location of the Calibration Label Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Accessing the Diagnostics Screen Accessing the Diagnostics Screen The Diagnostics tests are used to check the condition of the Autosampler hardware They are performed from the Diagnostics Screen which is comprised of four pages and is accessed as shown in the following procedure MAIN SCREEN READY E E sea on Po
102. ibes how to add other optional functions to the Main Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running Methods and Sequences Starting a Method Starting a Method This section describes the procedures for starting a method and includes e Starting the Active Method e Recalling and Starting a Stored Method Starting the Active Method You can start the active method from any screen by pressing the key on the keyboard As soon as you start the active method the Main Screen appears and displays information about the status of the active method The following screen shows typical status information for the default method Method00O which is now being run METHOD00 CHANGED DEFAULT SAMPLE VIAL 1 REP 1 OF 2 PIPETTING SAMPLE E CS ee Ee Be a Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Starting a Method Running Methods and Sequences Recalling and Starting a Stored Method MAIN SCREEN METHODOO READY E C M E A A Press F6 EELN from the Main Screen to go to the Directory Screen DIRECTORY SCREEN METHODOO STORED METHOD NAME LAST MODIFIED DEFAULT 02 FEB 1994 12 00 UTM TEST 02 FEB 1994 13 00 STORE STOR OR RECALL RCL A METHOD E u ES ee Using the cursor arrow keys move down through the list of stored methods until the carrot gt is next to the method you want to recall in
103. ibrants are injected for the previous method METHOD SCREEN METHOD FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 ENTER FIRST VIAL NUMBER 1 225 M BE ES I Be Ea a STANDARDIZE SCREEN METHOD STANDARDIZE TOTAL CALIBRANTS 5 REPS 1 INTERVAL 4 VIAL 10 11 12 13 14 VOL 5 5 5 5 5 ENTER TOTAL CALIBRANTS 1 7 ECN E J int Pviac fvor ft gt Injection Order Vial Vial Type Injection Volume Calibrant Calibrant Calibrant Calibrants are always run first Calibrant Calibrant Sample Sample Sample Sample Calibrant Calibrants are repeated every Calibrant 4th sample injection as defined Calibrant by the number 4 in the Calibrant INTERVAL data field in the Calibrant Standardize Screen Sample Sample Sample Sample Calibrant Calibrant Calibrant Calibrant Calibrant 1 2 3 4 5 6 7 8 9 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Active Method All Options Enabled Creating Methods and Sequences Setting Up Timed Events This section describes how to set up timed events TIMED EVENTS option enabled within a method METHOD SCREEN METHOD00 CHANGED FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 ENTER FIRST VIAL NUMBER 1 225 M E E BB Sc ee Press F5 to move to the Timed Events Screen The data field for the Time is highlighed TIMED EVENTS SCREEN METHOD0O0 CHANGED TIMED EVENTS EVENT TIME T E 1 T E 2
104. inary Data positive polarity Range of values for output 0 255 BIN NEGATIVE Binary Data negative polarity Range of values for output 0 255 EXTERNAL Choosing this selection simultaneously enables the serial ports A and B and disables the BCD port 2 To accept and save the change s and return to the Configure Screen press the key To cancel changes and return to the Configure Screen press the key 3 19 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Setting the Temperature of the Optional Peltier Accessory Setting the Temperature of the Optional Peltier Accessory This section describes how to set the temperature of the Peltier Accessory Make sure the Peltier tray is in the Autosampler and the cable coming from the tray is plugged into the Peltier connector on the rear panel of the Autosampler Accessing the Peltier Accessory Screen MAIN SCREEN READY ELT METH _ SEQ oir onre PRT From the Main Screen press F1 to move to the Peltier Accessory Screen PELTIER ACCESSORY CURRENT TEMPERATURE SET TEMPERATURE ENTER TEMPERATURE 0 C a Press F6 to set the temperature of the tray Use the numeric key to enter the value Press F7 if temperature control is not required To accept and save the change s and return to the Main Screen press the key To cancel changes and return to the Main Screen press the
105. ines to Waste Container 2 14 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making the External Tubing Connections Installation pg Connecting the Autosampler to the Column 1 Refer to Figure 2 7 Using nuts and ferrules recommended by the column manufacturer add a nut and ferrule to the end of the top section of tubing coming from the top cutout Note that the other section of tubing coming from the top cutout is labelled Pump 2 Thread the nut and ferrule into the column To column i ZA _ DS Labelled Pump FROM PUMP amp TO COLUMN Top cutout Column FROM PUMP amp TO COLUMN LY FLUSH DRAIN Figure 2 7 Connecting the Autosampler to a Column 2 15 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Making the External Tubing Connections Connecting the Autosamplerto the Pump 1 You can connect the other section of tubing coming from the top cutout labelled Pump directly to the pump or you can insert an in line device between the pump and the Autosampler Table 2 7 lists some common types of in line devices Figure 2 8 shows two types of in line devices 2 Adda nut and ferrule to the end of the tubing going to the pump or to the in line device Use a nut and ferrule recommended by the pump manufacturer
106. ing the end going to the pump Use nuts and ferrules recommended by the pump manufacturer or if you are inserting an in line device between the Autosampler and the pump use nuts and ferrules compatible with the device 8 Rethread the nut and ferrule going to the injector into port 2 on the injector valve Fin ger tighten the nut as far as it will go Take the 1 4 wrench and tighten the nut an addi tional 1 8 turn 9 Do not connect the other end to the pump or in line device until after you reinstall the injector valve Nut Ferrule Tubing amp Figure 7 22 Correct Orientation of Nut and Ferrule on Tubing Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Replacing the Waste Tubes Maintenance Replacing the Waste Tubes If you have not already done so remove the injector valve from the Autosampler as described in Removing the Injector Valve section in this chapter 1 Refer to Figure 7 23 which shows the location of all the ports on the bottom of the injec tor valve and their connections Sample loop connected Pump port 2 to ports 1 and 4 Column port 3 Waste vent port 5 Figure 7 23 Bottom View of the Injector Valve 2 Select the appropriate replacement waste capilliary vent tubes nuts and ferrules from Table 7 6 Table 7 6 Replacement Vent Capilliary Tubes Nuts and Ferrules Replacement Part Stainless Steel Part No Bio App
107. ing this option are on the previous page TIMED EVENTS Option METHOD OPTIONS STANDARDIZE on TIMED EVENTS off DERIV DILUTE off METHOD EDIT off RANDOM SAMPLING off SERIAL DILUTION off SELECT TIMED EVENTS OPTION ON OFF KON CEA E E A E E The TIMED EVENTS option allows you to control relay contact closures which in turn con trol auxiliary instruments like a column switching valve or a fraction collector When actu ated the relays close for two seconds then reopen This option allows you to activate two timed events TE 1 and TE2 up to 20 times during a method The relay contact closures TE1 and TE2 are located on the Input Output Terminal on the right side panel of the Autosampler and are described in Chapter 2 of this manual To add the TIMED EVENTS option press F1 XM Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Defining System Parameters DERIV DILUTE Option METHOD OPTIONS STANDARDIZE on TIMED EVENTS off DERIV DILUTE off METHOD EDIT off RANDOM SAMPLING off SERIAL DILUTION off SELECT DERIVATIZE DILUTE OPTION Lorr En fetch oof ft ft F1 F2 F3 F4 The DERIV DILUTE option allows you to e Perform derivatizations by adding up to 5 different reagents to the sample vials e Perform dilutions by adding diluent to the sample vials Note 1 Reagents and diluent are only added to sample vials and not to via
108. ining a Random Priority Method Creating Methods and Sequences As you move to and highlight each field a description of the information required for that field is displayed in the message line Use the numeric keys to make changes The information required and the acceptable ranges for each of the fields in the Random Priority Screen is summarized here NOTE Pressing the F8 REA function key will cancel all of the changes made to the Random Priority Screen FIRST LAST VOLUME REPLICATES Number of the first sample vial Number of the last sample vial Injection volume of the sample Replicates per sample vial 1 225 1 225 0 1 2500 uL 1 99 TIME The analysis time 0 00 999 minutes To add another item press either the key at the last data field or the key The system adds item 2 and uses the same settings as item 1 as shown in the next screen To change the settings use the cursor arrow and numeric keys PRIORITY RANDOM SAMPLING ITEM FIRST LAST VOLUME REPS TIME gt 1 3 3 10 1 2 3 3 10 1 ENTER THE FIRST VIAL NUMBER 1 225 EE Dw 0 10 0 10 You can continue to add and modify items in this manner up to a maximum of 99 The next screen shows the first three items in a completed Random Priority screen In the items shown here the Autosampler would sample from vials 3 then 6 8 and then 12 PRIORITY RANDOM SAMPLING ITEM FIRST LAST VOLUME REPS 1 3 10 1 gt 2 8 10 1 12 10 1 ENTER THE FIRS
109. ion 1 Isthe autosampler set for the correct mode fixed or loop NOTE If your autosampler is set for fixed loop mode the poor reproducibility is not due to the autosampler If this is the case check other components pump detector etc in your LC system Check excess volume and air cushion settings and try revert ing to the default values Check the sample syringe speed setting If this is set too high it can cause cavitation especially for viscous samples For mixed samples check the reagent mixing out of vial parameter F or viscous samples this should be set to the slowest speed For mixed samples check the reagent mixing into vial parameter and make sure it is adequate for your sample size To change the parameter values refer to Appendix B in this manual Restore factory settings by pressing the F8 key from the Config ure Screen see Defining System Parameters in Chapter 3 of this manual Chemical interaction See Ghost Peaks carryover below Peak doubling or Sample volume too large Inject sample prepared in mobile splitting phase with a total volume lt 15 of volume of first peak Check or replace column Replace injector valve rotor seal as in Chapter 7 of this man ual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Table Performance Problems Troubleshooting Performanca
110. ion Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining a Sequence Procedure for Defining a Sequence MAIN SCREEN METHODOO READY E E M A sea oe oneal erry 4 Press F5 E amp 6 from the Main Screen to go to the Sequence Screen The Method Number for SET 1 is highlighted SEQUENCE SCREEN SEQUENCE SET METHOD INJECTIONS gt 1 0 DEFAULT 1 Message line ENTER METHOD NUMBER 0 19 C ee CEG To change the default method for SET 1 use the numeric keys to type in a new method number If you do not want to create another SET press the key and the number and name of the method you typed in will be accepted and you will return to the Main Screen To create another SET press either the key or the key The screen changes to reflect the new method in SET 1 and adds an entry for SET 2 as shown in the next Sequence Screen on the facing page Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining a Sequence Creating Methods and Sequences Procedure for Defining a Sequence continued When you press the key or the key to add another set the system uses the same method as in the previous set Use the numeric keys to type in a new method number for the new set SEQUENCE SET METHOD INJECTIONS gt 1 3 UTM 5 2 3 UTM 5 ENTER METHOD NUMBER 0 19 EEM A OE Continue to add sets until you have entered all of the meth
111. ion Screen BC D Port Output The BCD port supports two output formats the BCD format and the Binary Data format The BCD format is a two digit format 00 99 and the Binary Data format is a three digit format 000 255 If you intend to program the Autosampler so that all control is done at the instrument and you only need to output vial numbers use e PerkinElmer cable Part No N293 0340 when connecting to Turbochrom or e PerkinElmer cable Part No N293 0341 when connecting to the 1022 Integrator or e PerkinElmer generic cable Part No N293 0342 when connecting to some other device the pin outs for this cable are provided on the next page If you are connecting to the BCD port using one of these cables you need to select the BCD NEGATIVE option on the Communication Screen 3 16 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Communications Options Configuring the Autosampler Pin assigments for PerkinElmer Generic BCD Cable Part No N293 0342 Figure 3 2 shows the PerkinElmer Generic BCD cable and the pin assignments N293 0342 rein cor eco om E pce o em e o o p e f oa o a e oe 800 08 o ee oa i o me eoo a e voe ow a Figure 3 2 Pin Assignments for Generic BCD Cable 3 17 Artisan Technology Group Quality Instrumentation
112. isan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Creating New Methods Recalling a Method from the Directory continued MAIN SCREEN METHOD0O1 STORED UTM TEST READY E C M E EA Gd At this point press F2 from the Main Screen to access the Method Screen You can modify the method information by changing the fields directly on the Method Screen or by changing the fields on the screens which can be accessed from the Method Screen Standardize Derivatiza tion Dilution or Timed Events Refer to Figure 4 3 on the facing page for a summary of how to access these screens Store the method under a new name and method number as described in the previous section Creating a New Method within the Active Method Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Creating New Methods awit xin on e M finia AE t HE H E AWILTINOA HH HH H HH OHH HH SIIOAO XIN HEO HE ke a ee a t AWMOA Ht E HH HH HH o WIA LSUIA 0A IdNVS 1na GSYOLS O0GOHLAW L L epa O i Fa NOHARIC a3avyo1 3g LSNW E ES Ee EXEC AE E OHH OH H OR AIWWIL LOWS HE H HH HH SFTIOAOXIN HE HE E H JNNTOA HE HE E E HoH WIA 1OA IdNVS Al 3d GAYOLS OOGOHLAW SS ES ED at eO HEHE HEHEHE TON re a p i NOILNIIA ea
113. iv Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figures Figure 1 1 Figure 1 2 Figure 1 3 Figure 1 4 Figure 1 5 Figure 1 6 Figure 2 1 Figure 2 2 Figure 2 3 Figure 2 4 Figure 2 5 Figure 2 6 Figure 2 7 Figure 2 8 Figure 2 9 Figure 2 10 Figure 2 11 Figure 2 12 Figure 2 13 Figure 2 14 Figure 2 15 Figure 3 1 Figure 3 2 Figure 4 1 Figure 4 2 Figure 4 3 Figure 6 1 Figure 6 2 Figure 6 3 Figure 6 4 Figure 7 1 Figure 7 2 Figure 7 3 Figure 7 4 Figure 7 5 Figure 7 6 Figure 7 7 The Series 200 LC Autosampler 1 2 Front Right Side View of Autosampler 1 5 Front Left Side View of the Autosampler showing the Dual Syringe System 1 6 Rear Panel of the Autosampler 1 7 Keyboard and Display 1 8 Screen Hierarchy Diagram 1 10 Removing the Packing Foam 2 7 Power Input Module exploded view 2 9 Fuse Location 2 10 AC Line Cord Selections 2 12 Typical Tubing Connections in an LC System 2 13 Connecting Waste Linesto Waste Container 2 14 Connecting the Autosamplerto a Column 2 15 Connecting the Autosamplerto a Pump 2 16 Location of the Injector Valve Port 2 18 Connecting the Sample Needle 2 19 Connecting the Flush Line Tubing to the Solvent Container 2 20 The Series 200 LC Autosampler Terminal Connectors 2 21 Connecting a Wire to the ModularConnector 2 23 System Connection Diagram Series 200 LC Autosam
114. l PerkinElmer Sales and Service office Routine Inspection and Suitability Test The Series 200 Autosampler housing should also be inspected weekly through the injection valve access door and the top sample access door for signs of liquid leaks Prior to any sam ple analysis a system suitability test which closely resembles the intended assay should be performed to ensure that the LC system is operating within established criteria e g peak resolution peak asymmetry precision retention time column plate count pressure limits signal noise ratio etc While the Universal Test Mix UTM Part No 0089 0893 can be used for a system check we recommend that you develop a separate system suitability test and acceptance criteria for each of your assays Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Safety and Regulatory Information Hazardous Chemicals Hazardous Chemicals Before using mobile phase solvents you should be thoroughly familiar with all hazards and safe handling practices Observe the manufacturer s recommendations for use storage and disposal These recommendations are normally provided in the material safety data sheets MSDS supplied with the solvents may be hazardous or may become hazardous after completion of an analysis The responsi ble body e g Lab Manager must take the nec essary precautions to ensure that the surrounding workplace and that the i
115. l bal SWIL 1N34 3 SLNJA 3 A NIL aa 4O01S O0dGOHLAW NOILVZILVAIWAG AEO AEO Oo HEH HH HE IA VAUALNI SdJY SLNVHEIVO IWLOL AZIGHYVGNVLS GSYOLS O0GOHLAW Pt e at a ais man HH HH HH HH AWIL SALVOIMdSsY AWNIOA LSV LSHIs GaYyOLS OOGOHLAN Screens tons Summary of the Method Opt Figure 4 3 4 23 Guaranteed 888 88 SOURCE www artisantg com Artisan Technology Group Quality Instrumentation Creating Methods and Sequences Editing a Stored Method Editing a Stored Method This section describes how to retrieve a stored method from the Autosampler Directory modify it and save it while a different active method is running When editing a method you can leave the edit session to perform other operations on the Autosampler and then return and continue editing without losing any of your changes MAIN SCREEN METHODO1 STORED UTM TEST READY E CS BS Gd To edit a stored method press F6 SARE from the Main Screen The Directory Screen similar to the one below appears DIRECTORY SCREEN METHODO1 STORED METHOD NAME LAST MODIFIED carrot gt 0 DEFAULT 02 FEB 1994 12 00 1 UTM TEST 02 FEB 1994 13 00 Message line STORE STOR OR RECALL RCL A METHOD C A SS A Press F1 and the following Edit Screen appears Note that the method which appears in the screen will correspond to the last method recalled during the previous edit session EDIT SCREEN EDIT 00 STORED F
116. l tubing connections between the Autosampler and other instruments making up an LC system Loop 1 to 4 From Pump eee RN EEN Series 200 Autosampler To Column j Helium Supply Bottom View of the Injector Valve Drain 3 x 3 Column 3 2 In Line Solvent Filter Optional gt LC 135 235C aa 200 LC Pump Py Solvent Systems Kit Diode Array Detector n eee A cn aera i Spiral Wrap P N 0998 4970 Figure 2 5 Typical Tubing Connections in an LC System 2 13 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Making the External Tubing Connections Connecting the Drain Hoses to the Solvent Waste Container 1 Refer to Figure 2 6 which shows the location of the drain hoses on the left side of the Autosampler 2 Place the free ends of both drain hoses into the solvent waste container Important Both drain hoses rely on gravity to carry away waste solvent Make sure to place the solvent waste container lower than the Autosampler gt FROM PUMP amp AE TO COLUMN J FLUSH _ DRAIN x ff To Solvent Waste from flush port ris from flush port To Solvent Waste from drip tray Figure 2 6 Connecting Waste L
117. left and right sides as well as the back oe p pE i y A SS ee A i 4 Figure 7 7 Removing the Autosampler Cover Replacing the Tubing There are three main sections of transfer tubing in the Autosampler e Solvent reservoir to flush solenoid length 4 ft e Flush solenoid to sample syringe T length 150 mm e Sample syringe T to sample needle length 5 ft When replacing the tubing cut the length of tubing you need from the Teflon Transfer line Part No N293 1139 which is supplied in a single 10 ft long section Replacing the Solvent Reservoir to Hush Solenoid Tubing When you replace this section of tubing you should also replace the solvent filter Refer to Figure 7 8 1 Cut anew 4ft section of tubing from the Transfer Line N 293 1139 2 Adda nut Part No 0992 0381 and reverse ferrule Part No 0992 0382 to the end of the tubing going to the flush solenoid inlet as shown in Figure 7 8 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Changing the Transfer Tubing Maintenance Nut Flush solenoid Flush solenoid inlet Reverse ferrule Sample Syringe T 1 16 in Flush C line tubing jf i a j Solvent Filte
118. lications Part No 2 4 in Vent 2 4 in Vent capilliary _ C N2931085 o mana3 O tubes Rheodyne short nut 0990 4956 N930 2676 Pkg of 5 Rheflex Rheodyne long nut 0990 4974 Nuts and Ferrules Rheodyne ferrule 0990 4947 3 Using a standard 1 4 wrench remove each nut and ferrule connecting the waste vent tubes to ports 5 and 6 4 Slide the one nut and one ferrule over each tube Orient each nut and ferrule as shown in Figure 7 24 5 Oneat atime insert the tube into port 5 or 6 whichever is appropriate on the bottom of Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Replacing the Waste Tubes the injector valve Push the tubing into the port until it stops Finger tighten the male nut as far as it will go Take the wrench supplied and tighten the nut an additional 1 2 turn 6 Completely remove the nut and ferrule Compare the tube with the attached ferrule to the tube without the ferrule Theleading edge of the tube with the ferrule should be tapered towards the tubing There should also be about 1 8 inch 3 mm of tubing that protrudes beyond the ferrule 7 Repeat steps 4 and 5 for the remaining waste tube 8 Rethread the nut and ferrule on each tube into its corresponding port Finger tighten the nut as far as it will go Take the wrench supplied and tighten the nut an additional 1 8 turn You will insert the free ends of the waste tubes from po
119. ll not start Series 200 LC Autosampler line Plug Series 200 LC Autosampler cord is not plugged into AC out line cord into the AC outlet let Power switch is off Turn on the power switch Blown fuse Replace the fuse as described in Chapter 7 of this manual Nodisplay Power lightis Screen saver enabled Press any key on Autosampler runs slow Sample compartment door is Close sample door open Autosampler will not Noready in connection Connect controlling external inject device pump to ready in Refer to Making Input Output Termi nal Connections section in Chap ter 2 of this manual Autosampler performs Improper external connections Check I O connections Refer to injection but external LC Making Input Output Terminal devices do not function Connections section in Chapter 2 of this manual Not connected as serial port A or B Autosampler will not com Not configured for external con Refer to Defining the Communi municate with external trol cations Options section in Chap controller TC4 or 1022 ter 3 of this manual Autosampler will not No flush solvent Fill solvent reservoir rime syringes will not fill syring Flush syringe has been allowed Hold solvent reservoir above the to dry out level of the flush syringe to increase head pressure Solenoid valve fails Call PerkinElmer service Worn or defective flush syringe Replace flush syringe Artisan Technology Group Quality
120. ls designated as containing calibrants Note 2 In order to perform dilutions you need to insure that the dilution sample tray is installed in the Autosampler To add the DERIV DILUTE option press F2 F3 or F4 F2 Ifyou select the SING single option the Autosampler will perform the derivatiza tion or dilution operation on the first sample in the method and then inject that sam ple F3 If you select the BTCH batch option the Autosampler will first perform the deriva tization or dilution operation on all of the samples in a method it will then return to the first sample and begin the injections F4 Ifyou select the D O derivatizaion dilution only option the Autosampler will only perform the dilution or derivatization on the samples It will not inject the samples Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining System Parameters Configuring the Autosampler METHOD EDIT Option METHOD OPTIONS STANDARDIZE on TIMED EVENTS off DERIV DILUTE off METHOD EDIT off RANDOM SAMPLING off SERIAL DILUTION off SELECT METHOD EDIT OPTION ON OFF CE a E A A A E The METHOD EDIT option allows you to retrieve a stored method from the directory mod ify it and save it while a different active method is running You can also leave the edit session to perform other operations on the Autosampler and then return and continue edit ing without losing any of you
121. magnetic Compatibility EMC Safety and Regulatory Information Safety and Regulatory Information Electromagnetic Compatibility EMC United States This product is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Safety and Regulatory Information Symbols and Conventions used in this Manual Symbols and Conventions used in this Manual In this manual the following graphic symbols and special text formats are used to set apart important safety information cause personal injury if precautions are not f Waming A warning indicates an operation that could followed cause instrument damage if precautions are Caution A caution indicates an operation that could not followed Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Symbols used on the Instrument Safety and Regulatory Information Symbols used on the Instument There are two different types of warning symbols that appear on the instrument tion on the instrument that could cause per sonal injury if precautions are not followed This can be any type of hazard When you see this symbol refer to the safety pages in the manual for furt
122. move buffers flush the system with water followed by methanol or isopropanol Remember to change the flush solvent from methanol or isopropanol to water before using buffers Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Warnings and Precautions Installation Corrosion The following corrosion precautions apply to the standard stainless steel autosampler and the biocompatible titanium autosampler Standard Autosampler All parts of the autosampler that contact mobile phase from the pump and flush solvent are made of stainless steel Teflon and glass Some of these materials are extremely sensitive to acid chlorides If you have questions about your mobile phase or flush solvent contact a Per kinElmer representative Refer to Table 2 1 Table 2 1 Solvents Which May Corrode the Series 200 LC Autosampler Aqua Regia 80 HCI 20 HNO3 Bromine Hydrogen Peroxide Freon 12 wet Anhydrous Chloride Chlorinated solvents Dichloromethane H ydrofluorosilicic Acid 20 Sulfuric Acid Conc Hydrobromic Acid 20 Hydrochloric Acid 20 and 37 Ferric Chloride Hydrofluoric Acid 20 50 and 75 Ferrous Chloride Copper Chloride Mercuric Chloride Dilute Biocompatible Autosampler All parts of the Series 200 BIO LC Autosampler that contact the mobile phase from the pump and flush solvent are made of titanium fluoropolymers or glass This makes the Series 200 BIO L
123. mpler to stop processing any additional sample vials and terminate the analysis if it encounters a missing sample vial If a reagent or calibrant vial is missing the method or sequence will terminate To accept and save the change s and return to the Instrument Setup Screen press the key To cancel changes and return to the Instrument Setup Screen press the key 3 15 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Defining the Communications Options Defining the Communications Options This section describes how to select either the seriel communication ports or the Binary Coded communicaton port on the rear panel of the Autosampler It also describes how to specify the output format for the BCD port Thelocation of these ports on the rear panel of the Autosampler is shown here for your convenience Serial Ports Figure 3 1 Location of the Communication Ports on the Rear Panel of the Autosampler Serial Ports If you intend to control the Autosampler using Turbochrom through a 600 Series Link box you will need PerkinElmer cable Part No N293 0343 If you intend to control the Autosampler using a 1022 Integrator you will need Perkin Elmer cable Part No N293 0344 If you are connecting through the serial ports using either one of the above cables you will need to select the EXTERNAL CONTROL option on the Communicat
124. n Date 09938912 September 1995 Any comments about the documentation for this product should be addressed to User Assistance PerkinElmer 710 Bridgeport Avenue Shelton CT 06484 4794 Or emailed to info perkinelmer com Notices The information contained in this document is subject to change without notice Except as specifically set forth in its terms and conditions of sale PerkinElmer makes no warranty of any kind with regard to this document including but not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of PerkinElmer Inc Copyright 2004 PerkinElmer Inc Produced in the U S A Trademarks Registered names trademarks etc used in this document even when not specifically marked as such are protected by law PerkinElmer is a registered trademark of PerkinElmer Inc Teflon and Tefzel are registered trademarks of E I duPont de Nemours Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Electro
125. n the screen saver off press F2 EHA To increase the screen contrast press F4 ENJ To decrease the screen contrast press F6 EEJ To accept and save the change s and return to the Instrument Setup Screen press the key To cancel changes and return to the Instrument Setup Screen press the key 3 12 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining System Parameters Configuring the Autosampler Setting the System Clock From the Instrument Setup Screen press F4 to move to the System Clock Screen SYSTEM CLOCK SCREEN SYSTEM CLOCK DATE DA MO YR 12 15 94 TIME 24HR CLK 15 06 foay Junta fvear A E wn E a To set the day press F1 ZSA The first character field in DATE 12 becomes highlighted Use the numeric keys to enter the new day To move to the month character field 15 in DATE press F2 and use the numeric keys to enter the new month Set the year and the hour and minute values in the same way that is by pressing the appropriate function and using the numeric keys to enter the new val ues To accept and save the change s and return to the Instrument Setup Screen press the key To cancel changes and return to the Instrument Setup Screen press the key 3 13 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Defining System Parameters
126. nation problems in your laboratory provide a relatively dust free envi ronment Make sure that the following gases or vapors are not present at levels exceeding federal state and local ordinances for continuous human exposure Flammable Explosive Toxic Caustic Corrosive Make sure that your laboratory environment consists of the following temperature and humidity levels e Ambient temperature between 10 C and 30 C e Constant relative humidity between 20 and 80 without condensation Use care when working with hazardous solvents or solvents that produce hazardous by products interfere with the detector performance should be considered hazardous to someone who is continuously exposed to the same vapors Waming Solvent vapor levels that are high enough to Adequate Bench Space Provide bench or table space to accommodate the dimensions of the autosampler listed in the specification section The weight of the Series 200 LC Autosampler is 21 kg 46 Ibs The bench must also support the weight of the detector and other instruments in the system Provide space at the rear of the autosampler for air circulation and provide access space on both sides of the autosampler Waste Solvent Disposal Provide a properly labeled chemical waste container in a safe and vented place Make sure that it is within all of the specified safety requirements for your location Wear gloves eye protection and a laboratory coat when handling or dis
127. nce is run in the order defined during the setup of the sequence Priority Method The priority method is similar to a method in that it may also consist of a single injection or series of injections of several samples and or calibrants It differs from the active method however in that there is a only a single priority method and you can interrupt the active method or sequence with the priority method the active method resumes as soon as the priority method is completed In addition any changes you make to the priority method are automatically saved so that if you turn the power off on the Autosampler you will not lose the changes Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preface Using the Manual Conventions Conventons The following conventions are used in this manual e Aninstrument screen display is pictured as a single lined box METHOD00 STORED DEFAULT E CS ee Be a e Instructions for using the softkey functions are displayed in the text as press F2 to e Instructions for using hard keys on the instrument keyboard are displayed in text as press the key Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Customer Service Preface Using the Manual Customer Service Company Name and Address PerkinElmer Instruments 761 Main Avenue Norwalk CT 06859 USA For Sales Assistan
128. nd down through the list of entries The following example shows the three most common types of screen lists the Directory Screen Sequence Screen and the Timed Events Screen carrot List of the methods stored in the Autosampler s Directory List of the methods or SETs comprising a sequence List of all the timed events for a particular method in this case Method01 DIRECTORY SCREEN METHOD01 STORED METHOD NAME 0 DEFAULT gt 1 UTM TEST 02 FEB 1994 13 00 2 UTM ASSAY 03 MAR 1994 12 00 STORE STOR OR RECALL RCL A METHOD C ES ee ee LAST MODIFIED 02 FEB 1994 12 00 SEQUENCE SCREEN SEQUENCE SET METHOD INJECTIONS 1 3 TESTI 5 gt 2 5 UTM ASSAY 12 3 8 ASSY4 35 ENTER METHOD NUMBER 0 19 a E ee EG TIMED EVENTS SCREEN METHOD01 STORED TIMED EVENTS EVENT TIME T E 1 T E 2 4 0 23 YES YES 0 24 YES YES 0 25 YES YES CTi ES ES EEN ee ee gt 5 6 1 15 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Using the Keyboard and Display Responding to Screen Prompts Torespond toa Y N prompt appearing in the Message line of a screen such as the one shown in the following Method Screen press the 4 numeric key to select Y and the 9 to select N METHOD SCREEN METHOD0O0 STORED FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 Message line OVERWRITE EXISTING METHOD Y N M BS ES Bd cd es
129. ne provides information on available options To add the STANDARDIZE option press F1 XM To accept the entry and move to the next field you can use either the cursor arrow keys or the key The key works in the same way as a tab key moves from left to right and down See Describing the Method Options section beginning on the next page for a brief over view of the remaining options For detailed information on using each of these options in an active method priority method or random priority method refer to Chapter 4 of this manual To accept and save the change s and return to the Instrument Setup Screen press the key To cancel changes and return to the Instrument Setup Screen press the quit key Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining System Parameters Configuring the Autosampler Describing the Method Options This section gives a brief description of the options provided by the Method Options Screen As you move to and highlight each option field directions for selecting the option appear in the message line and the soft keys change to reflect the choices available for that option For detailed information on using each of these options in a method refer to Defining the Active Method in Chapter 4 of this manual STANDARDIZE Option The STANDARDIZE Option allows you to add up to 7 calibrants to a method Directions for enabl
130. nel allows quick plumbing inspection and easy servicing e Maintenance log coded by the serial number of the autosampler including autosampler injection cycles e Instrument log records history of operation for quick diagnosis and servicing to get you up and running quickly e Extensive service diagnostics e Sampler module can be completely removed to lab bench for maintenance without having to unstack modules Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Main Components of the Series 200 LC Autosampler Main Components of the Series 200 LC Autosampler The Series 200 LC Autosampler consists of the following main components A brief descrip tion of each of the components follows Refer to Figure 1 2 for the location of the following components Keyboard and Display All instrument functions are accessed using the keyboard Status information is shown on the screens appearing in the display An overview describing how to perform some basic operations using keyboard and display is provided in Using the Keyboard and Display sec tion of this chapter Input Output Connector Panel The connector panel consists of six contact closure connectors for automated instrument con trol of devices like an LC pump LC detector and a data handling system Sample Tray The Autosampler accepts eight different types of sample trays The trays hold sample and calibran
131. new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED D a gaa tia Contact us 888 88 SOURCE sales artisantg com www artisantg com
132. nges to one of the Standardize Derivatization Dilution or Timed Events Screens that you access using one of the soft keys you need to press the key to save those changes and return to the Edit Screen above To leave the Edit mode to view for example the status of the current method or sequence press the key To return to the Edit mode again press F6 EIN from the Main Screen then press F1 If you make a mistake entering numeric values in any of the fields on the screen s press the key and retype the number To store the changes under a new method number and name press F6 from within the Edit Screen and follow the procedure described in the Creating a New Method from within the Active Method section in this chapter Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining a Sequence Defining a Sequence A sequence is a series of stored methods that are linked together This section contains the information you need to define a sequence Before you begin you need to know the rules for defining a sequence and how to identify the methods that comprise the sequence Rules for Defining a Sequence 1 If you have not already done so you need to create and store in the Autosampler s Direc tory all of the methods that you intend to include in the sequence Procedures for creat ing and storing methods are contained in the Creating Ne
133. nications Options 3 16 Serial Ports 3 16 BCD Port Output 3 16 Accessing the Communication Screen 3 18 Selecting the Communication Option 3 19 Setting the Temperature of the Optional Peltier Accessory 3 20 Accessing the Peltier Accessory Screen 3 20 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configunng the Autosampler This chapter describes how to configure your Series 200 LC Autosampler It includes the fol lowing information Powering up the Autosampler Reviewing the Default Injection Performance Parameters Defining System Parameters Adding Method Options Setting the Screen Contrast Setting the System Clock Checking the Hardware Options Defining the Error Recovery Mode Defining Communications Options Setting the Temperature of the Optional Peltier Accessory Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Powering Up the Autosampler Powering Up the Autosampler Press the power switch located on the lower right side of the Autosampler to the ON posi tion The Copyright and Self Test screen first appears on the Liquid Crystal Display The display is located directly above the keyboard on the front panel As soon the self test is completed th
134. nral erry From the Default Main Screen press F7 to move to the Configure Screen CONFIGURE SCREEN CONFIGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION LOG INSTRUMENT LOG inst cowmfoiac Pca Proc a ark oer 2 Press F3 EY to enter the diagnostic mode As soon as you press F3 the following prompt appears as the last line in the Configure Screen STOP amp ENTER DIAGNOSTICS Y N Press the 1 numeric key to select Y and the first page of the Diagnostics Screen appears Note that the count field is highlighted This represents the number of times a test will be performed PAGE 1 OF THE DIAGNOSTICS SCREEN KPD1 KEYPAD DISPLAY TEST 1 KPD2 KEYPAD DISPLAY TEST 2 PROM PROM TEST RAM RAM TEST BBUP BATTERY BACKUP TEST COUNT 01 E keo2 prompram Peeve __ more Go to the next page to view all of the pages in the Diagnostics Screen and directions for moving between the pages Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Accessing the Diagnostics Screen Calibration and Diagnostics Moving Between Pages of the Diagnostics Screen PAGE 1 OF THE KPD1 KEYPAD DISPLAY TEST 1 DIAGNOSTICS SCREEN KPD2 KEYPAD DISPLAY TEST 2 FLSH FLASH TEST RAM RAM TEST BBUP BATTERY BACKUP TEST COUNT 01 A kroz rus Pram Pesue A PAGE 2 OF THE PELT PELTIER COMMUNICATIONS TEST DIAGN
135. nstru ment operators are not exposed to hazardous levels of toxic substances chemical or biologi cal as defined in the applicable Material Safety Data Sheets MSDS or OSHA ACGIH or COSHH documents Venting for fumes and disposal of waste must be in accordance with all national state and local health and safety regulations and laws f Waming Some chemicals used with this instrument Definitions in Waming for Hazardous Chemicals Responsible body Individual or group responsible for the use and maintenance of equipment and for ensuring that operators are adequately trained per IE C 1010 1 Amendment 2 draft Operator Person operating equipment for its intended purpose per IEC 1010 1 Amendment 2 draft OSHA Occupational Safety and Health Administration United States ACGIH American Conference of Governmental Industrial Hygienists COSHH Control of Substances Hazardous to Health U nited Kingdom Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Temperature Humidity and Environment Safety and Regulatory Information Temperature Humidity and Environment Caution This instrument is designed for indoor use ated area The Series 200 LC Autosampler operates most efficiently under the following conditions ambient temperature is 10 to 30 C ambient relative humidity is 20 to 80 without condensation altitude is in the range 0 to 2000 meters
136. nteed 888 88 SOURCE www artisantg com Appendix A Sample Trays Available Tray Types 225 Vial Part Number N293 0043 Accepts 0 2 mL Microvials 225 P N N930 2136 205 Vial Part Number N293 0042 Accepts 0 2 mL Microvials 225 P N PR ee Accepts 1 8 mL vials 10 P N N930 7017 a os ar ae ot YN ON ODO SN J 14 j J A AS SSF SSF RS SF Na Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Available Tray Types Appendix A Sample Trays 85 Part Number N294 0041 Accepts 1 8 mL vials 80 P N N930 7017 Accepts 7 0 mL vials 5 P N N930 2134 Accepts 1 1 8mL vials 80 P N N930 7017 2 0 2 mL Microvials 80 P N N930 2136 Dilution Tank ae a n Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix A Sample Trays Available Tray Types 25 Part Number N294 0040 N SA Na y NY i J C A Vy J IN N D fe N Accepts 1 8 mL vials 25 P N N930 2134 See ee a A AA ne N J So F Pa fr x 3 N ae EN C J oa N i p ON N gt a a C j p Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix B Changing the Performance Parameters This appendix describes how to change the default injection performance parameters listed in Cha
137. nteed 888 88 SOURCE www artisantg com Setting up the Serial Dilution Screen Appendix C Serial Dilutions Setting up the Senal Dilution Screen The following procedure describes how to set up the Serial Dilution screen to perform the dilution shown in Figure C 1 MAIN SCREEN METHODOO READY E CS ET SB Sn Press F3 from the Main Screen to go the Serial Dilution screen The data field for the first source vial is highlighted SERIAL DILUTION SCREEN SERIAL DILUTION TARGET VOL 0 TRANSFER SOURCE VOLUME TARGET MIX gt 1 1 1 50 0 Message line _gm ENTER FIRST SOURCE VIAL 1 225 amp TANK ETA EN vor _Js o J s_ new 2 In this example the first transfer will actually be 500 uL of diluent from the diluent tank to target vial in position 2 To select the tank press F2 SERIAL DILUTION TARGET VOL 0 TRANSFER SOURCE VOLUME TARGET MIX To accept the entry and move to the VOLUME field use the gt 1 TANK 1 1 0 cursor arrow keys ge ENTER TRANSFER VOLUME 0 2500 UL Ec Cs BG Ee ES 0 Note that as you move from field to field a description of the information required for each field Using the numeric keys type in the 500 uL volume of diluent to be added to the target vial 2 appears in the message line Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix C Serial Dilutions Setting up the Serial Dilution Screen SERIAL DILUT
138. ods for the Sequence Remember the maximum number of methods in a Sequence is 10 Note If you make a mistake and add the wrong method remove it by pressing the key To link the sequence to the method in Set 1 press F6 EIS To select the SET position in the Sequence Screen press F1 HES To delete the entire sequence so that you can create a new sequence press F8 BERS Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining a Priority Method Defining a Priority Method A priority method is similar to an active method in that it may also consist of a single injec tion or series of injections of several samples and or calibrants It differs from the active method however in several important ways How is the Priority Method different from an Active Method e Thereisa single priority method For the active method you can store up to 19 different methods e You can interrupt the active method or sequence and run the priority method As soon as the priority method is completed the active method or sequence will resume e Any changes you make to the priority method are automatically saved so that if you turn the power off on the Autosampler you will not lose the changes When do you run the Priority Method While you can run a priority method any time it is usually reserved for those occasions when you need to interrupt an active method
139. of sample and buffer to be withdrawn from the vial is first injected into the sample vial This prevents a vacuum from developing when multiple samples are withdrawn from the vial 11 Flush Volume The volume of flush liquid used for each 0 2500 uL cyde during the pre and postinjection flush operation 12 Speed of the Flush The speed at which the flush syringe Syringe picks up buffer from the buffer reservoir and ejects it through the sample needle 13 Preinjection Flush The number of flush cycles before sample Cycles is injected 14 Post injection Flush Thenumber of flush cycles after sample is Cycles injected Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com The Default Performance Parameters Appendix B Changing the Performance Parameters Acceptable Value or ear Range Parameter Description 15 E 15 Endof Method of Method The number of flush cycles at the end ofa number of flush cycles at the end of a Flush Cycles method 16 Hard Key Cycles The number of times the flush syringe will cycle when you press the flush key 17 Reagent Mixing The speed of the flush syringe when it Speed In picks up sample plus reagent from the vial through the sample needle for mix ing Note that for viscous samples you should set the speed to VSLO very slow or SLOW 18 Reagent Mixing The speed of the flush syringe when it Speed Out ejects sample plu
140. of these accessories is to use the modular connector Part No 0999 6640 that is supplied in the Start Up Kit To make a connection 1 Refer to Figure 2 13 Cut off about 10 mm of plastic sleeve from each of the two wires Insert the two wires into the two center positions labelled R and G in the inverted top piece 2 Combine the top and bottom pieces Secure them with the Phillips screw 3 Plug the modular connector into the O panel connector on the Series 200 LC Autosam pler Figure 2 13 shows a typical connection between the Autosampler and the Series 200 Pump 4 Refer to Figures 2 14 and 2 15 for examples of typical 1 O connections between the Autosampler and other instruments making up an LC system Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Making Input Output Terminal Connections Installation Preparation of Interconnecting Cable ends using Modular Connector 0999 6604 STEP 1 STEP 2 Cut off 10 mm of plastic sleeve and insert the Combine the top piece with the two wires into the two center positions bottom piece and secure them labeled R and G with the Phillips Screw _Phillips Screw Top Piece Bottom Piece Cable to The Model 1020 1022 or Turbochrom Interconnecting Cable 20 ft supplied Figure 2 13 Connecting a Wire to the Modular Connector Artisan Technology Group Quality Instrumentation
141. ogy Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Changing the Sample and Flush Syringes 5 For thesample syringe slide the plunger into the sample syringe plunger slot Thread the top of the sample syringe into the sample T 6 Replace the solvent filter in the solvent reservoir 7 Reconnect the Autosampler to the electrical supply Switch on the power to the Autosampler Press the key on the keyboard and flush solvent through the system until there are no bubbles in the syringes or transfer tubing To ensure that there are no bubbles in the transfer tubing inspect closely the tubing near the top of the sample needle Table 7 3 Replacement Sample Syringes Sample Syringe Injection Range Replacement Part No 50 uL 0 1 20 uL 0992 3304 100 uL 0 1 65 uL 0992 3305 250 uL 0 1 195 uL 0992 3270 500 uL 0 1 430 uL 0992 3306 1000 uL 1 0 880 uL 0992 3307 2500 uL 1 0 2475 uL 0992 3219 7 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Replacing the Sample Needle Maintenance Replacing the Sample Needle 1 Position the sample needle assembly over the injector valve port using the PARK func tion on the Configure Screen as shown here MAIN SCREEN READY E E sea on A erry Press F7 from the Main Screen to move to the Configure Screen CONFIGURE SCREEN CONF
142. oid Problems e Follow good laboratory practice e Useonly high quality purity HPLC grade solvents Note Mixtures of organics and water once degassed remain in a degassed state for longer periods of time than pure organics This minimizes bub ble formation in the transfer lines In general depending on your mobile phase strengths are 3 1 methanol water 1 1 methanol water or your specific mobile phase without buffer e Use filtered solvents to prevent the tubing in the LC system from becoming clogged e Usean in line filter for example PerkinElmer Part No 0990 3606 between the pump and the injector valve e Use only clean passivated tubing for the solvents e Follow the operating and warning instructions in this manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Guidelines for Maintaining the Autosampler Routine Maintenance and Inspection Carry out the following tasks regularly to avoid problems e Use only degassed and filtered HPLC grade solvents for the flushing solution e Make sure the flushing solution container is clean and rinse it out with fresh solvent before filling e Donot let the waste container overfill Empty it regularly ensuring the solvent is dis posed of correctly e Check the filters regularly and clean or replace them as required e Check the system regularly for leaks Where necessary tighten joints or replace dam aged pa
143. on METHOD OPTIONS STANDARDIZE on TIMED EVENTS off DERIV DILUTE off METHOD EDIT off RANDOM SAMPLING off SERIAL DILUTION off SELECT SERIAL DILUTION OPTION ON OFF E cs ee ee TheSERIAL DILUTION option allows you to perform a serial dilution starting from a single calibrant and then making a series of vial to vial transfers No injections are performed between the vial to vial transfers Note 1 Unlike methods you can only have a single serial dilution program Note 2 The key and the key are disabled in the serial dilution To add the SERIAL DILUTION option press F 1 For more information refer to Appendix C Setting up Serial Dilutions in this manual 3 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Defining System Parameters Setting the Screen Contrast and Screen Saver From the Instrument Setup Screen press F2 to move to the Screen Contrast Screen SCREEN CONTRAST SCREEN ABCDEFGHIJKLMNOPQRSTUVWXYZ1234567890 ABCDEFGHIJKLMNOPQRSTUVWXYZ1234567890 ABCDEFGHIJKLMNOPQRSTUVWXYZ1234567890 ABCDEFGHIJKLMNOPQRSTUVWXYZ1234567890 SCREEN SAVER ON CONTRAST 8 CO A A OS A a By default the screen saver option is ON This ensures that after 15 minutes of keyboard inactivity the screen s back light will go out Note pressing any key on the keyboard will turn the screen s back light on again To tur
144. one end of the 5 ft tubing section Unscrew the nut and ferrule connecting the old section of tubing to the sample syringe T Remove the nut and ferrule from the end of the tubing Thread the tubing back through the path shown in Figure 7 9 all the way to the point where it connects to the union on the sample needle Unscrew the PEEK nut connecting the old sample tubing to the union Connect the end of the replacement tubing with the PEEK nut tothe union Thread the replacement tubing back through all of the clamps identified in Figure 7 9 to the sample syringe T When you reach the two adjacent clamps on the top of the PC board be sure and loop the transfer tubing three times through the clamps as shown Add a nut and reverse ferrule to the free end of the tubing and thread it into the sample syringe T Replacing Part of the Sample T to Needle Tubing If you only need to replace a portion of the tubing at or near the point where it enters the union on the sample needle do the following 1 2 3 4 5 Unscrew the PEEK nut connecting the sample tubing to the union Remove the PEEK nut from the end of the tubing and cut off the old section Thread the tubing back through the 2 adjacent clamps on top of the PC board see Figure 7 9 to free up an additional length of tubing Each loop yields about 5 inches of tubing Rethread the tubing back through the clamps to the union Replace the PEEK nut on the f
145. ontinue until diluent sample and internal standard have been added to the remaining target vials 43 through 60 A portion of the mixture specified in the VOLUME field in the Method Screen and shown on the facing page as 20 uL may be injected Whether or not the final dilution is injected and when the injection occurs depends on the DERIV DILUTE option selected from the Method Options Screen described in the Adding Method Options section in Chapter 3 of this manual B To Injection Valve 72 9 4 78 7 28 8 2 64 6 66 7 G6 60 0 POQQOQOQMOOEO novas Q 6 000909000000 o 000000000 M Q O O urpera DOOOOO QOE Sua Figure 4 2 Dilution Tray Setup for Pipetting 100 uL of Diluent 50 uL of Sample and 25 uL of Internal Standard 4 16 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Active Method All Options Enabled Creating Methods and Sequences Procedure and Screen Entries for Example 2 From the Main Screen press F2 to move to the Method Screen From the Method Screen press F1 GWM to create a new active method Fill in the fields with the entries shown here Note that the VOLUME specified in this screen refers to the portion of the mixture in the target vial that is injected METHOD02 CHANGED FIRST LAST VOLUME REPLICATES TIME 41 60 20 1 1 0
146. or sequence that is already in progress to insert priority sample s How do you define a Priority Method The procedures and screens used to define the priority method are virtually identical to the ones used for defining the active method described in the first two sections of this chapter Defining the Active Method Samples Only and Defining the Active Method All Options Enabled The only difference is in how you access the Priority Screen and the titles of the screens Both of these differences are shown in the diagram on the facing page Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining a Priority Method Creating Methods and Sequences MAIN SCREEN METHODOO READY E E a SB CS Ga Press F8 to go to the Priority screen PRIORITY SCREEN PRIORITY FIRST LAST VOLUME REPLICATES TIME 1 1 10 1 10 ENTER FIRST VIAL NUMBER 1 225 M a ES ES cd es Note Any method options you enabled for active method and stored methods in Adding Method Options in Chapter 3 also apply to the Priority Method The top level Priority Screen shown here assumes that you have enabled all of the method options At this point you can modify the priority method information by changing the fields directly on the top level screen or by changing the fields on the screens which are accessed from the Priority Screen Standardize Derivatization Dilution or Timed Even
147. ple T Tubing 7 17 Sample Transfer Tubing Path 7 19 Pump and Column Connectionsto the Autosampler 7 20 Removing the Drain Tubes from the Waste Drain 7 21 Top of the Injector Valve Assembly 7 22 Front View of Injector Assembly 7 23 Injector Valve Disassembly 7 25 Corect Position of Rotor Seal 7 26 Guides on Adjusting Screw and Body of Injector 7 26 Orientation of Injector Valve Stem in the Injector Valve Assembly 7 28 Bottom View of the Injector Valve 7 30 Bottom View of the Injector Valve 7 31 Correct Orientation of Nut and Ferule on Tubing 7 32 Bottom View of the Injector Valve 7 33 Correct Orientation of Nut and Ferule on Tubing 7 34 Bottom View of the Injector Valve 7 35 Correct Orientation of Nut and Ferule on Tubing 7 36 Dual Syringe System 8 6 Location of Waste Lines on Injector Valve 8 7 Serial Dilution Example C 2 Method Screen D 1 vi Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Preface Using the Manual The PerkinElmer Series 200 LC Autosampler is an advanced sample processor that can be used with virtually every liquid chromatography system or application If you use the Per kinE Imer Series 200 LC Pump you will find that the Autosampler front panel is almost identical to the one on the Pump In addition the Pump and the Autosampler share many common operating features that will
148. pler Series 200 LC Pump LC UV VIS Detector and a Data System 2 24 System Connection Diagram Series 200 LC Autosampler Series 200 LC Pump LC 235C Diode Amay Detector and a Data System 2 24 Location of the Communication Ports on the Rear Panel of the Autosam pler 3 16 Pin Assignments for Generic BCD Cable 3 17 Dilution Tray Setup for Pipetting 500 uL of Diluent and 100 uL of Sample 4 14 Dilution Tray Setup for Pipetting 100 uL of Diluent 50 uL of Sample and 25 uLof Intemal Standard 4 16 Summary of the Method Options Screens 4 23 Location of the Calibration Label 6 5 Rear Panel of the Autosampler 6 15 Location of the RDY IN Connector 6 19 Rear Panel of the Autosampler 6 25 Power Input Module 7 5 Fuse Location 7 6 Dual Pump System Sample and Flush Syringes 7 9 Removing the Needle Cartridge 7 12 Removing Replacing the Sample Needle 7 13 Removing the Injector Valve Cover 7 15 Removing the Autosampler Cover 7 16 v 9 95 Rev C Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Figure 7 8 Figure 7 9 Figure 7 10 Figure 7 11 Figure 7 12 Figure 7 13 Figure 7 14 Figure 7 15 Figure 7 16 Figure 7 17 Figure 7 18 Figure 7 19 Figure 7 20 Figrue 7 21 Figure 7 22 Figure 7 23 Figure 7 24 Figure 8 1 Figure 8 2 Figure C 1 Figure D 1 Replacing the Flush Line Tubing Solvent Filter and Flush Solenoid to Sam
149. port 6 Column port 3 A Waste vent port 5 Figure 7 19 Bottom View of the Injector Valve 2 Select the appropriate replacement tubes nuts and ferrules from Table 7 4 Table 7 4 Replacement Tubing Nuts and Ferrules Replacement Part Stainless Steel Part No Bio Applications Part No Tubing setions sections 0271 1441 20 cm 0271 1441 20cm 1 16 in O D in O D N930 2678 N930 2678 5 ft 1 16 in O D ft 1 16 in O D 0 007 in 1 D 0 007 in 1 D N293 1089 30 cm 1 16 in O D N930 2650 5 ft 1 16 in O D 0 007 in D 0 010 in 1 D 0089 1480 50 cm 1 16 in O D 0 007 in D Rheodyne short nut 0990 4956 N930 2676 Pkg of 5 Rheflex Rheodyne long nut 0990 4974 Nuts and Ferrules Rheodyne ferrule 0990 4947 3 Using a standard 1 4 wrench remove the nut and ferrule connecting the column tube to port 3 4 Slidethe nut and ferrule over the tube Orient the nut and ferrule as shown in Figure 7 20 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Replacing the Tubing to the Column 5 Insert the tube into port 3 on the bottom of the injector value Push the tubing into the port until it stops Finger tighten the nut as far as it will go Take the 1 4 wrench and tighten the nut an additional 1 2 turn 6 Completely remove the nut and ferrule The leading edge of the tube should be tapered towards the tubing Ther
150. posing of chemical waste Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Unpacking Your Series 200 LC Autosampler Unpacking Your Series 200 LC Autosampler Removing the Instrument from the Shipping Container 1 The weight of the autosampler is approximately 21 kg For convenience lifting points are provided in the packing foam at the front and rear of the autosampler 2 Grasp the autosampler at the lift points and carefully lift it so that it clears the packing foam 3 Check for obvious signs of damage that may have occurred during shipment I mmedi ately report damaged or missing items to the shipping carrier and PerkinElmer 4 Save the original packing in case you need to return the instrument Items Included When you open the shipping container the first box you will see is the Startup Kit Depend ing on your application you will receive either the Start Up Kit for the Series 200 LC Autosampler Part No N293 0305 or the Start Up Kit for the Series 200 BIO Compatible LC Autosampler Part No N293 0306 For N293 0305 refer to the Start Up Kit Packing List Part No 0993 6176 for N293 0306 refer to the Start Up Kit Packing List Part No 0993 6177 The part numbers in these lists should help you to identify items in the kits but do not use these part numbers to order replacements To order replacement parts refer to the PerkinElmer HPLC and CE Supplies Cat
151. priority method 4 30 Priority Method Screen 4 31 Pump connecting to the Autosampler 2 16 location of the pump tubing connection 2 16 pump tubing replacement part numbers 7 33 replacing the tubing to the pump 7 33 type of in lines devices between pump and Autosampler 2 17 R Random priority method 4 32 starting 5 8 Random Priority Screen 4 32 summary of data field requirements and ranges 4 33 Random sampling see also Random priority method adding the random sampling option 3 10 defined 3 10 Ready In Loopback Connector 6 19 Rear panel description 1 7 Recalling and starting a stored method 5 5 Rotor seal replacing 7 24 In 3 Guaranteed 888 88 SOURCE www artisantg com Index S Sample compartment door 1 5 7 15 Sample loop 7 30 changing 7 30 Sample needle calibrating 6 3 6 4 installing and connecting 2 17 2 19 replacing the sample needle 7 11 7 13 Sample needle assembly description 1 4 startup kit components 2 17 Sample syringe changing the sample syringe 7 8 description 1 6 injection ranges 7 10 location 1 6 7 9 replacement part numbers 7 10 sizes 1 17 7 10 Sample trays checking the tray type 3 14 description 1 4 A 1 A 4 Sample volume minimum 1 17 Sampling operation description 1 4 Saving changes to screens 1 16 new methods 4 20 Screen contrast setting 3 12 Screen defined function keys 1 8 1 12 Screen saver enabling 3 12 Screens Calibration Screen 6 3 Communications Screen 3 18 Configure Scr
152. pter 3 Configuring the Autosampler In order to change the parameters you need to do the following e Access the Performance Parameter Screen this screen is comprised of three pages e Move within and between the pages of the Performance Parameter Screen e Change the necessary parameters For your convenience the default performance parameters are repeated again herein Table B 1 The Default Perfomance Parameters Table B 1 Default Performance Parameters Default Acceptable Parameter Description Value Value or Range 1 Air Cushion Volume of air drawn up before sample 10 uL 1 99 uL Air cushion is used to prevent mixing of the flush buffer and the sample 2 Loop Mode Partial loop is partially filled with the Partial Partial Fixed volume specified during method setup Fixed loop fills up completely with sam ple If you change the default from par tial to fixed refer to Appendix D in this manual for the recommended sample loop and sample syringe sizes 3 Excess Volume Extra volume of sample drawn up during 10 uL 0 100 uL Sample sampling operation Note in the Fixed Loop Mode the sample volume drawn up is already ten times the loop volume The excess volume is then added to that vol ume 4 Sample Pump The size of the syringe used to draw up 250 uL 50 100 250 500 1000 Syringe Size the sample 2500 uL Artisan Technology Group Quality Instrumentation Guaranteed
153. r 1 8 in Tubing Part No 0990 3610 Figure 7 8 Replacing the Flush Line Tubing Solvent Filter and Flush Solenoid to Sample T Tubing To attach the new solvent filter to the other end of the flush line tubing first insert a sec tion of 1 8 in tubing into the solvent filter Then slide the 1 16 in flush line tubing into the 1 8 in tubing Firmly screw the solvent filter onto the end of the tubing 5 Thread the nut and ferrule end of the tubing into the flush solenoid inlet Place the end with the solvent filter into the solvent reservoir Replacing the Hush solenoid to Sample Syringe T Tubing 1 2 Cut a new 150 mm section of tubing from the Transfer Line N 293 1139 Add a nut Part No 0992 0381 and reverse ferrule Part No 0992 0382 to each end of the 150 mm tubing section Orient the each nut and ferrule as shown in Figure 7 8 Thread one end into the flush solenoid and the other into the sample syringe T as shown in Figure 7 8 7 17 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Changing the Transfer Tubing Replacing the Tubing from Sample T to Needle 1 Refer to Figure 7 9 on the facing page which shows the path of the transfer tubing from the sample syringe T to the union on the sample needle Cut a new 5 ft section of tubing from the Transfer Line N 293 1139 3 Adda PEEK nut 0992 0513 to
154. r Valve Under normal conditions the injector valve will perform thousands of injections before requiring any maintenance The two components of the injector valve most likely to require maintenance are the rotor seal and the stator face assembly respectively Replacing the Rotor Seal and Stator Face Assembly 1 Refer to Figure 7 14 Remove the three stator screws 2 Removethe stator Note that the stator face assembly usually comes off with the stator 3 Remove the stator ring 2 piece assembly 4 Pull the rotor seal off the pins Note Leave the isolation seal and bearing ring in place 5 Seeitem 2 in this section for reassembly Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Dismantling the Injector Valve Maintenance Shaft Assembly 8125 035 E Includes Shaft Rotor Pin Pressure Adjusting Screw l and Seal Pins 7725 034 B E Body 8125 076 Thrust Bearing 7125 042 Spring Washers 4 7010 014 Body Locating Pin 9010 015 a eee Position Sensing Switch 9010 018 60 Degree Stop Ring 9010 017 i i Aa Model 7725 only Needle Port Tube 7125 068 Tube Spring 7125 035 Bearing Ring 7010 006 Isolation Seal 7010 015 __1 D on Rotor Seal 7125 047 i i Stator Ring 9010 016 S Ff Stator Locating Pin 9010 014 Stator Face Assembly 7725 026 me
155. r and define all of your methods by filling in information on the screens that appear in the LCD You use the keys on the key board to enter the information on the screens and also to start or stop certain operations on the Autosampler As you move from one screen to another the screen defined function keys change The functions of the various keys are summarized in Table 1 1 on the next page You access all of the Autosampler screens starting at the top level Main Screen which is shown in the LCD in Figure 1 5 All of the Autosampler screens are shown in the screen hierarchy diagram in Figure 1 6 Screen Saver LED Screen Backlit Liquid Crystal Display METHODOO retu rn Function Keys Screen defined function keys Keyboard Figure 1 5 Keyboard and Display Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Using the Keyboard and Display Introduction Summary of the Keyboard Functions Table 1 1 Summary of Keyboard Functions Press a function key to select the corresponding screen defined soft key label displayed above it For example press F7 from the Main Screen to go to the Configure Screen flush Press to flush and clean the sample needle and transfer line This is active only when a method is not controlling the instrument Press pause if you want to add samples to the sample tray during a
156. r changes To add the METHOD EDIT option press F1 EJ Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Defining System Parameters RANDOM SAMPLING Option METHOD OPTIONS STANDARDIZE on TIMED EVENTS off DERIV DILUTE off METHOD EDIT off RANDOM SAMPLING off SERIAL DILUTION off SELECT RANDOM SAMPLING OPTION ON OFF KON a E A A A E The RANDOM SAMPLING option allows you to sample vials in a nonconsecutive or random manner For example by enabling this option you can program the Autosampler to pick up sample from vial 1 then move to and pick up sample from vials 12 through 18 then go back and pick up sample from vial 6 By adding the RANDOM SAMPLING option you replace the Priority Method with the Ran dom Priority M ethod Note 1 You cannot include calibrants perform dilu tions or derivatizations or program timed eventsin a Random Priority Method Note 2 Unlike methods you can only have one Random Priority Method Note 3 Enabling RANDOM SAMPLING does not affect your ability to add calibrants or perform dilu tions derivatizations or include timed events in the active or stored methods To add the RANDOM SAMPLING option press F1 EW 3 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining System Parameters Configuring the Autosampler SERIAL DILUTION Opti
157. ransfer entry number when prompted then press the key The carrot will move to the selected item Alternatively you can use the cursor arrow keys to scroll up and down the list To delete a tranfer entry select the item and press the key To permanently save changes to the screen without running the dilution press the key Torun thedilution later press the F3 from the Main Screen to enter the Serial Dilution screen then start the dilution by pressing either F 6 IRA or F 7 HEE To clear all the changes made to the Serial Dilution screen press the F 8 GW function key Summary of the Serial Dilution Data Field Requirements and Ranges The following is a summary of the data fields and their acceptable range of entries Data Field Description TARGET VOL The volume of sample in the first target vial 0 2500 uL To access this data field press F4 from the Serial Dilution screen SOURCE The first source vial 1 225 amp Tank To specify the diluent tank press F2 from the Serial Dilution screen VOLUME The volume to be tranferred from the source vial or diluent tank 0 2500 uL TARGET The target vial to receive the volume of material from the source vial or the diluent tank 0 2500 uL MIX The number of mix cycles or times the transferred material from the source vial or diluent tank will be mixed with the sample in the target vial 0 9 If there is O uL of sample in the target vial this should be set to 0 For best r
158. re incompatible the Priority Screen will appear with an error message as soon as you press the key For example if the last vial in the Priority Method is set at 225 and you have a standard 100 vial sample tray in the Autosampler the Priority Screen will appear as shown here To correct the error and clear the message use the cursor arrow keys to move to the LAST field and enter the correct number PRIORITY SCREEN PRIORITY FIRST LAST VOLUME REPLICATES TIME 1 225 10 1 1 PRIORITY SAMPLE VIAL gt 100 M ee ES ee ee Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Starting a Priority Method or Random Priority Method Running Methods and Sequences Interrupting the Active Method or Sequence and Running the Priority Method To insert the priority method into the active method or sequence press the key on the keyboard Refer to item 2 if you are interrupting the active method If you are interrupting a sequence refer to item 3 Interrupting the Active Method If the Autosampler is performing an operation on a method like sampling when you press the key the following Main Screen display appears The PPEND in the top line denotes that a priority method is pending and will start as soon as the Autosampler completes its current operation The method will resume once the priority method is completed METHOD03 STORED PPEND UTM SAMPLE VIAL 1 REP 1 OF 2 PIPETTI
159. ree end of the tubing and rethread it into the union Hushing the System Replacing the Cover 1 When you are done replacing the tubing reconnect the Autosampler to the electrical sup ply and switch on the power Press the key on the keyboard and flush solvent through the system until there are no bubbles in the sample tubing To ensure that there areno bubbles inspect closely the tubing near the point where it enters the union on the sample needle Replace the injector valve cover by reversing the procedure in item in this section Replace the Autosampler cover by reversing the procedure in item Fal in this section 18 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Changing the Transfer Tubing Maintenance a D g S Do D g D n i Q amp 3 9 gaye D U Do S gt D x j j o Z lt Via ar 7 E JD HT Jy 3 a a 7 TF 7 19 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Sample Transfer Tubing Path Figure 7 9 Maintenance Removing the Injector Valve Removing the Injector Valve Removing the Injector Valve Cover 1 Switch off the power to the Autosampler and disconnect it from the electrical supply 2 Follow the same procedure for removing the injector valve cover as described in Chang ing the Trans
160. rformance Parameters refer to Appendix B Changing the Per formance Parameters Depending on the types of vial caps you are using you may need to change the Vent Mode parameter refer to Appendix E Operating Guidelines for Derivati zations and Dilutions Two examples are presented here to show how to use the dilution feature on the Autosam pler In thefirst example there are 40 sample vials and 40 empty target vials Each sample vial contains a different volume of liquid You will program the Autosampler to e Add 500uL of diluent to each of the 40 target vials then e Pipet 100 uL of liquid from each of the 40 sample source vials into its corresponding tar get vial In the second example there are 20 sample vials 20 vials containing an internal standard and 20 target vials As in the first example each sample vial contains a different volume of liquid You will program the Autosampler to e Add 100 uL of diluent to each of the 20 target vials then e Pipet 50 uL of liquid from each of the 20 sample source vials into its corresponding tar get vial then e Pipet 25 uL of internal standard from each of the 20 standard vials into its corresponding target vial Each example will show the dilution tray setup and the corresponding screen entries 9 95 Rev C 4 13 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Example
161. rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Introduction Instrument Specifications AC voltage range Grounded 100 120 220 230 VAC 10 Grounded 240 VAC 6 10 Fasering See Installing the AC Line Cord section in Chapter 2 of this manual Environment This instrument is designed for indoor use It is not designed to operate in an explosive environment Ambient temperature 10 30 C Ambient relative humidity 10 80 without condensation Altitude 0 to 2000 meters Installation Category This instrument is able to withstand transient overvoltage according to Installation Category as defined in IEC 1010 1 Pollution Degree This instrument will operate safely in environments that con tain nonconductive foreign matter to Pollution Degree 2 in IEC 1010 1 ture range seregeconditons Dimensions Height 400 mm 15 8 in Width 318 mm 12 5 in Depth 483 mm 19 in 1 18 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Installation Wamings and Precautions 2 2 Solvents 2 2 Solvents with Low Boiling Points 2 2 Buffers 2 2 Conosion 2 3 Solvents with Auto Ignition Temperature Below 110 C 2 4 Preparing Your Laboratory 2 5 Required Air Quality 2 5 Adequate Bench Space 2 5 Waste Solvent Disposal 2 5 Unpacking Your Series 200 LC Autosampler 2 6 Items Included
162. rts e If you do not run solvent through the Autosampler for a period of 24 hours flush the sample and flush pump syringes and the tranfer tubing with isopropanol before run ning any samples Viewing the Instument Log Periodically check the Instrument Log as described in section Viewing the Instrument Log in Chapter 6 of this manual This lists the number of valve and injection cycles performed by the Autosampler You can use this information to help set up a maintenance schedule Switch off all instruments in the system 1 2 Disconnect the Autosampler from the elec trical supply 3 Follow the maintenance instructions exactly as described in this chapter Waming Before starting any maintenance work Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Replacing a Fuse Maintenance Replacing a Fuse Locating and Removing the Power Input Module 1 Switch off the power to the Autosampler and disconnect it from the electrical supply 2 Refer to Figure 7 1 The fuseis contained in the Power Input Module located on the rear of the Autosampler Serial Ports A B Power Input Module Figure 7 1 Power Input Module 3 4 Refer to Figure 7 2 Remove the Power Input M odule cover by inserting a small flat blade screwdriver into the notch and carefully prying off the cover Remove the voltage selector card from the Power Input Mod
163. rts 5 and 6 into the remaining free top arm of the Y shaped drain tube coming from the flush port after you reinstall the injector valve Nut Ferrule Tubing Figure 7 24 Correct Orientation of Nut and Ferrule on Tubing Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting 8 Troubleshooting Table 1 Performance Problems 8 2 Troubleshooting Table 2 General Instrument Problems 8 8 Troubleshooting Table 3 Display Eror Messages 8 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting 8 This chapter describes how to solve some basic performance and instrument problems Table 1 lists the most frequently encountered performance problems and what you can do to correct them In addition illustrations are provided at the end of the Table 1 to assist you in troubleshooting Table 2 lists general instrument problems Table 3 lists display error messages their causes and solutions Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Troubleshooting Table 1 Performance Problems Troubleshooting Table 1 Performance Problems Performance Possible Cause s Check and or remedy Poor Area and Hei
164. run This prevents the needle arm and sample pump from moving until you press pause again to resume operation priority Press priority to start a priority method The priority method will start after the current injection completes Press start to initiate a sequence or the active method The sequence will start if the methods within a sequence are linked otherwise the active method will start stop Press to stop the autosampler Pressing stop raises and then flushes the sample needle quit Press quit to cancel any changes you have made to the screen or one of the pages within a screen and return to the previous screen return Press return to save changes you have made to the current screen or one of the pages within the screen and return to the previous screen Press enter to accept a typed value and move to the next position or field Press CE to clear a typed value and redisplay the original value insert Press to insert a duplicate of the current step below the current step and increment the steps following it by one for example when adding methods in a sequence Press to delete the current step and decrement the steps following it by one Numeric Keys Use numeric keys 1 9 Use numeric keys 0 and 1 no for numeric entry and yes to answer to yes no prompts Use the cursor arrow keys to accept a typed value and to move between SO fields within a screen or page of a screen Artisan Technology Group
165. s reagent through the sample needle back into the vial for mix ing Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix B Changing the Performance Parameters Accessing the Performance Parameter Screen Accessing the Performance Parameter Screen DEFAULT MAIN SCREEN METHODOO READY E E M Pf sea on A erry From the Default Main Screen press F7 to move to the Configure Screen CONFIGURE SCREEN CONFIGURE INST INSTRUMENT SETUP COMM COMMUNICATIONS DIAG DIAGNOSTIC TESTS CAL CALIBRATION LOG INSTRUMENT LOG DIAG ae pA Press F1 to move to the Instrument Setup Screen INSTRUMENT SETUP SCREEN OPT METHOD OPTIONS SCRN SCREEN CONTRAST SAVER PERF PERFORMANCE PARAMETERS CLK SYSTEM CLOCK TRAY TRAY TYPE RECV ERROR RECOVERY fort EN EA four rravprecv Press F3 to move to the first page of the Performance Parameter Screen The data field for the first parameter LOOP MODE will be highlighted INJECTION PERFORMANCE PARAMETERS INJECTION PERFORMANCE PARAMETERS LOOP MODE partial VENT MODE DELAY TIME 0 AIR CUSHION NEEDLE LEVEL 10 EXCESS VOL TIME SAVER Off SELECT LOOP MODE PARTIAL FIXED Eu Ee a a a E E Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Moving in the Performance Parameter Screen Appendix B Changing the Performance Parameters Moving in the Performance Parameter
166. sampler s Directory Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating New Methods Creating Methods and Sequences Creating a New Method by Recalling a Method from the Directory This procedure describes how to recall a method from the Autosampler directory so that you can modify it and save it under a new name and number MAIN SCREEN METHODOO READY E C M E A Gd Press F6 EEEN from the Main Screen to go to the Directory Screen DIRECTORY SCREEN METHODOO STORED METHOD NAME LAST MODIFIED DEFAULT 02 FEB 1994 12 00 UTM TEST 02 FEB 1994 13 00 STORE STOR OR RECALL RCL A METHOD Ci ES ee Using the cursor arrow keys move down through the list of stored methods until the carrot gt is next to the method you want to recall in this example 1 METHODOO STORED METHOD NAME LAST MODIFIED DEFAULT 02 FEB 1994 12 00 carrot UTM TEST 02 FEB 1994 13 00 l UTM ASSAY 03 MAR 1994 12 00 Message line STORE STOR OR RECALL RCL A METHOD Ci m El Press F4 and the message line will change to the following RECALL METHOD 1 Press the key and the message line will display the following prompt OVERWRITE ACTIVE METHOD Y N N Press the 4 numeric key to change the default to Y This will replace the current active method which is Method0O0 with Method01 from the directory The Main Screen will appear as shown on the next page Art
167. see if liquid is coming out of the needle guide on top of the injector valve Remove the vent waste tubes from ports 5 and 6 1 If liquid flows out of ports 5 and 6 and no liquid comes out of the needle guide replace the waste line tubes If liquid still flows out of the needle guide rebuild the injec tor valve Refer to Chapter 7 in this manual for directions on replacing the waste vent tubes and rebuilding the injec tor valve Worn injector valve seals Check the instrument log see Viewing the Instrument Log in Chapter 6 of this manual to deter mine if the number of injection cycles gt 5000 If the number is gt 5000 replace the injector valve rotor seal and stator face assembly as described in Chapter 7 of this manual Incorrect vial closure Check vial caps used on sample vials especially for large sam ples Use only recommended vials Check the needle height and be sure the needle is not hitting the bottom of the vial If neces sary set a new needle level in the vial Refer to Table 3 1 Default Perfor mance Parameters jn Chapter 3 of this manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Troubleshooting Table 1 Performance Problems Performance Problems Possible Cause s Check and or remedy Improper performance Verify that the performance param parameters eters are set properly for your appli cat
168. st sample vial number is highlighted METHOD SCREEN METHOD0O0 CHANGED FIRST LAST VOLUME REPLICATES TIME 1 1 10 1 10 Message line f gt ENTER FIRST VIAL NUMBER 1 225 Te i Sc ee ee Using the numeric keys on the keypad type in the number of the first sample vial as directed in the message line METHOD0O0 CHANGED FIRST LAST VOLUME REPLICATES TIME To accept the entry and 1 4 10 1 10 move to the next field use N the cursor arrow keys ENTER THE LAST VIAL NUMBER 1 225 M e a a I E M ee As you move to and highlight each field a description of the information required for that field is displayed in the message line Use the numeric keys to make changes The information required and the acceptable ranges for each of the fields in the Method Screen is summarized here NOTE Pressing the F1 REM function key will cancel_all changes made to the Method Screen FIRST Number of the first sample vial 1 225 LAST Number of the last sample vial 1 225 VOLUME Injection volume of the sample 0 1 2500 uL REPLICATES Replicates per sample vial 1 99 TIME The analysis time 0 00 999 minutes To retain these changes press the key This also takes you back to the Main Screen and saves the changes until you turn the power off on the Autosampler Then when you restore power the default values for the method will also be restored To permanently store the changes refer to the Creating New Methods section in this chapter
169. steel needle guide you set aside earlier in the procedure Remov ing the Injector Valve in this section Slide the top cover over the injector valve assembly and under the thumbscrews on the left side of the sample compartment Tighten the thumbscrews Reconnect all the external tubing connections Reconnect the Autosampler to the electrical supply and switch on the power Press the key on the keyboard and flush solvent through the system until there are no bubbles in the sample tubing To ensure that there areno bubbles inspect closely the tubing near the point where it enters the Teflon union on the sample needle Important If you have changed the type of injector valve or if you have rebuilt the injector valve you need to do the following 1 Recalibrate the sample needle as described in the Calibrating the Sample Needle sec tion in Chapter 6 of this manual 2 Reset the valve cycles to zero as described in the Viewing the Instrument Log sec tion in Chapter 6 of this manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Changing the Sample Loop Changing the Sample Loop If you have not already done so remove the injector valve from the Autosampler as described in Removing the Injector Valve section in this chapter Important If you have also changed the loop mode perfor mance parameter from partial to fixed refer to Appendix D
170. t vials as well as dilution and derivatization vials or tanks For a complete list of available tray types refer to Appendix A in this manual The Autosampler identifies the tray type by reading the pattern of fingers on the back of the tray When you load a tray these fingers slide into a sensor which is recessed in the back wall of the tray well Sample Needle Assembly This assembly consists of a sample needle that moves up and down Z direction and is mounted in a transport mechanism that moves backwards and forwards and left to right XY direction The sample needle picks up sample or calibrant from the vial and depending on the method performs dilutions and or derivatizations and injects the sample onto the col umn It is connected to and works in conjunction with the Injection Valve Assembly and the Dual Syringe System The Injection Valve Assembly is accessed through the Injection Valve Access Door The location of the Dual Syringe System is shown in Figure 1 3 During the sampling operation e Thetransport mechanism positions the sample needle above the sample vial e A cushion of air is drawn up into the needle e The needle is lowered to just pierce the vial septum At this point the system verifies that the vial is present e The needle is fully lowered into the vial and the required sample volume is drawn up e Theneedle is raised clear of the vial and another cushion of air is drawn up e Thetransport mechanism moves the n
171. ter Screen It also gives the screen page number where the parameter is located Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Changing Information on the Screen Appendix B Changing the Performance Parameters Table B 2 Performance Parameters P age Number 13 Preinjection Flush Cycles FLUSH PUMP PERFORMANCE PARAMETERS FLUSH CYCLES PRE Page 3 of 3 14 Post injection Flush Cycles FLUSH PUMP PERFORMANCE PARAMETERS FLUSH CYCLES POST Page 3 of 3 15 End of Method Flush Cycles FLUSH PUMP PERFORMANCE PARAMETERS END OF METHOD CYCLES Page 3 of 3 16 Hard Key Cycles FLUSH PUMP PERFORMANCE PARAMETERS HARD KEY CYCLES Page 3 of 3 17 Reagent Mixing Speed In REAGENT MIXING SPEED IN Page 2 of 3 18 Reagent Mixing Speed Out REAGENT MIXING SPEED OUT Page 2 of 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix B Changing the Performance Parameters Changing Information on the Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix C Senal Dilutions This appendix describes how to define a serial dilution It assumes that you have enabled the Serial Dilution option as described in the Adding Method Options section in Chapter 3 of this manual The Serial Dilution option allows you to perform a serial dilution starting from a single cali
172. ter the number of replicates METHOD00 STORED STANDARDIZE TOTAL CALIBRANTS 5 REPS 14 INTERVAL 4 VIAL 1 2 3 4 5 VOL 5 5 5 5 5 Message line gt ENTER REPLICATES PER VIAL 1 9 ECN J a Pvc fvor Tt A To move to other fields use the cursor arrow keys As you move to and highlight each field a description of the information required for that field is displayed in the message line Use the numeric keys to make changes The information required and the acceptable ranges for each of the fields in the Method Standardize Screen is summarized here TOTAL CALIBRANTS Total number of calibrants 1 7 REPS Number replicates per calibrant vial 1 9 INTERVAL Defines how often the calibrants are run 1 99 VIAL The vial number for each calibrant 1 225 VOL The calibrant injection volume for the corresponding vial 0 1 2500 uL To save these changes press the key This also takes you back to the Method Screen and saves the changes until you turn the power off on the Autosampler When you restore power the default values for the method will also be restored To permanently store the changes refer to the Creating New Methods section in this chapter Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining the Active Method All Options Enabled Example of Sample and Calibrant Injection Order The following example shows the order in which samples and cal
173. tg com Creating Methods and Sequences Creating New Methods Creating New Methods This section describes how to create new methods by permanently storing changes to the active method You can do this either from within the active Method Screen or by recalling an existing method from the Autosampler directory Note When you recall a method from the Autosam pler directory that method replaces or over writes the current active method If you need to create a new method while the Autosampler is running the active method you can do so by copying an existing method stored in the Autosampler s directory This proce dure is described in the Editing a Stored Method section of this chapter 4 18 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating New Methods Creating Methods and Sequences Creating a New Method from within the Active Method METHOD SCREEN METHOD0O0 CHANGED DEFAULT FIRST LAST VOLUME REPLICATES TIME 10 2 5 0 1 4 ENTER FIRST VIAL NUMBER 1 225 Mi u ES Bd cl u 4 Press F6 from within the Method Screen The message line changes and prompts you for the method number The highlighted number that appears corresponds to the next available method number 1 19 METHOD0O0 CHANGED DEFAULT FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 Message line STORE METHOD 04 M BS ES I Be Erc ee Press the key to accept the method number shown or
174. the sample 2 Loop Mode Partial loop is partially filled with the Partial Partial Fixed volume specified during method setup Fixed loop fills up completely with sam ple If you change the default from par tial to fixed refer to Appendix D in this manual for the recommended sample loop and sample syringe sizes 3 Excess Volume Extra volume of sample drawn up during 10 uL 0 100 uL Sample sampling operation Note in the Fixed Loop Mode the sample volume drawn up is already ten times the loop volume The excess volume is then added to that vol ume 4 Sample Pump The size of the syringe used to draw up 250 uL 50 100 250 500 1000 Syringe Size the sample 2500 uL 5 Sample Pump The speed at which the sample syringe MEDM Syringe Speed picks up and dispenses sample The for all speed varies with the size of the sample syringe pump syringe Note that for viscous sam sizes ples you should set the speed to VSLO very slow or SLOW 6 Sample Pump The pause time following each sample 2 seconds 0 10 seconds Syringe Pause syringe movement This allows the air segment between the sample and the flush buffer to equilibrate Delay Time This is used for gradient methods It 0 00 0 00 999 minutes allows you to delay the sample injection minute by this amount of time to compensate for the dead volume between the pump and the Autosampler Time Saver If this feature is enabled On it allows Off On Off the Autosampler to get the ne
175. ting the active method 5 4 Stator face replacing 7 24 System clock setting 3 13 T Technical assistance P 5 Timed events see also Timed Events Screen defined 3 7 Timed Events Screen 4 9 setting up 4 9 4 10 Guaranteed 888 88 SOURCE www artisantg com Transfer tubing changing 7 15 7 18 path through Autosampler 7 19 section lengths 7 16 Troubleshooting general instrument problems 8 8 8 9 performance problems 8 2 8 7 Tubing connections description of required connections 2 13 typical LC system connection 2 13 V Voltage selector card location 2 8 selecting the voltage 2 9 Ww Warnings and Precautions 2 2 Weight of the Autosampler 1 18 2 6 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Index In 5 Index Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com rtisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com PerkinElmer precisely PerkinElmer 710 Bridgeport Avenue Shelton CT 06484 4794 U S A Internet http www perkinelmer com email info perkinelmer com PerkinElmer is a registered trademark of PerkinElmer Inc Part Number 0993 8912 C Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com A rtisan Artisan Technology Group is your source for quality TecmoogyGroup
176. tosampler within the last 24 hours flush the system with isopropanol 7 Check the syringes and tubing for air bubbles If bubbles are present flush the system with isopropanol then again with the appropriate degassed flush solvent 8 Select the proper tray for analysis Appendix A lists all of the available tray types 9 Select or create the method or sequence Creating methods and sequences is described in Chapter 4 of this manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Powering Up the Autosampler Running Methods and Sequences Powering Up the Autosampler Press the power switch located on the lower right side of the Autosampler to the ON posi tion The Copyright and Self Test screen first appears on the Liquid Crystal Display The display is located directly above the keyboard on the front panel As soon as the self test is completed the Main Screen appears COPYRIGHT AND SELF TEST SCREEN SERIES 200 LC AUTOSAMPLER COPYRIGHT C PERKIN ELMER 1995 SOFTWARE REVISION 1 00 RUNNING SELF TEST DEFAULT MAIN SCREEN METHODOO READY E C0 ee Ee Be a The Default Top Level Main Screen The Main Screen provides access to all of the Autosampler s functions The soft keys dis played along the bottom of the default Main Screen shown here correspond to the functions that are available when you first power up the Autosampler Chapter 3 in this manual descr
177. ts Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating Methods and Sequences Defining a Random Priority Method Defining a Random Priority Method This section shows how to define a random priority method It assumes that you have enabled the Random Sampling option as described in the Adding Method Options section in Chapter 3 of this manual The random priority method allows you to sample vials in a non consecutive or random man ner Each itemin a random priority method represents a single tube or multiple tubes in a consecutive series You may define up to 99 items MAIN SCREEN METHODOO READY E E a SB CS Gd Press F8 to go to the Random Priority screen The data field for the first sample vial num ber is highlighted RANDOM PRIORITY SCREEN PRIORITY RANDOM SAMPLING ITEM FIRST LAST VOLUME REPS TIME 1 1 10 1 0 10 Message line ENTER THE FIRST VIAL NUMBER 1 225 mew new Using the numeric keys on the keypad type in the number of the first sample vial for the first item as directed in the message line PRIORITY RANDOM SAMPLING ITEM FIRST LAST VOLUME REPS TIME To accept the entry and gt 1 3 1 10 1 0 10 move to the next field use A the cursor arrow keys ENTER THE FIRST VIAL NUMBER 1 225 mew new Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Def
178. twe ore 2 If a failure occurs the following screen appears KPD1 KEYPAD DISPLAY TEST 1 PASS FAIL eect fours fousztwe ore Press the key to redisplay page 1 of the Diagnostics Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics Keypad Test 2 This test is designed to test the operation of each of the keypad keys If a key works it is highlighted If a key fails to highlight call your PerkinElmer Service Engineer PAGE 1 OF THE DIAGNOSTICS SCREEN KPD1 KEYPAD DISPLAY TEST 1 KPD2 KEYPAD DISPLAY TEST 2 FLSH FLASH TEST RAM RAM TEST BBUP _ BATTERY BACKUP TEST COUNT 01 E keo2 rush Pram Peaue _ wore Press F2 KRA to start the test The following screen appears QUIT F1 F2 F3 F4 F5 F6 F7 F8 F9 FLSH INS DEL 7 8 9 PAUS 4 5 6 PRTY 1 2 3 STRT gt 0 STOP v ENT CE Press each key on the keyboard and verify that the corresponding label highlights on the screen lf a label fails to highlight contact your PerkinElmer Service Engineer Press the key twice to redisplay page 1 of the Diagnostics Screen The first time you press the key it highlights the RET field on the above screen the second time will redisplay page 1 of the Diagnostics Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibr
179. uaranteed 888 88 SOURCE www artisantg com Running the Diagnostic Tests Calibration and Diagnostics Clear Battery RAM Caution This test will delete all methods the sequence VAN configuration and calibration parameters PAGE 3 OF THE DIAGNOSTICS SCREEN OUT OUTPUT RELAYS TEST PART READ FIRMWARE PART NUMBERS CLRM CLEAR ALL METHODS CRAM CLEAR BATTERY RAM BCD _ BCD OUTPUTS TEST COUNT 01 M CS Ce Ee ME ETS Press F4 to clear the battery backup RAM The following screen appears DELETE ALL METHODS SEQUENCE CONFIGURATION AND CALIBRATION PARAMETERS CRAM CLEAR BATTERY RAM ECE ECE E E A E E 2 Press F1 to run the test or press F2 IMN to redisplay page 3 of the Diagnostics Screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Running the Diagnostic Tests BCD Outputs Test To perform the BCD Output Tests first connect the PerkinElmer cable Part No N293 0340 or similar tothe BCD port on the rear panel of the Autosampler The location of the BDC port is shown in Figure 6 4 Usea DVM to check the outputs PAGE 3 OF THE DIAGNOSTICS SCREEN OUT OUTPUT RELAYS TEST PART READ FIRMWARE PART NUMBERS CLRM CLEAR ALL METHODS CRAM CLEAR BATTERY RAM BCD _ BCD OUTPUTS TEST COUNT 01 M CS Fe Ee TS Press F5 to run the BCD Output Test The following screen appears BCD OUTPUT TEST LINE 80 40 20 ST
180. ule and store in a safe place Be careful not to change the voltage selector setting Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Replacing a Fuse Screw Fuse Block Single Fuse Screw Fuse Block Voltage Selector Card Voltage Selector Power Input Arrow Module Cover Dual Fuses Figure 7 2 Fuse Location 2 Changing the Fuse s 1 Remove the old fuse s For a single fuse configuration select the replacement fuse from Table 7 1 For a dual fuse configuration select the replacement fuses from Table 7 2 Table 7 1 Single F use Configuration Table Use only 3AG 1 4 x 1 1 4 250V Rated Fuses Voltage Selector Replacement fuse 1 6 amp Slow Blow 0998 1613 1 6 amp Slow Blow 0998 1613 0 8 amp Slow Blow 0999 1671 0 8 amp Slow Blow 0999 1671 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Replacing a Fuse Maintenance Table 7 2 Dual Fuse Configuration Table Use only 5 x 20 mm 250V Rated F uses Voltage Selector Replacement fuse P N 220 0 8 A Type TT 0999 1670 240 0 8 A Type TT 0999 1670 2 Slide the fuse s into the fuse holder See Figure 7 2 3 Replace the voltage selector card 4 Reinsert the power input module cover back into the power input module Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www
181. uring the Autosampler Powering Up the Autosampler 3 2 The Default Top Level Main Screen 3 2 Reviewing the Default Performance Parameters 3 2 Defining System Parameters 3 5 Adding Method Options 3 6 Setting the Screen Contrast and Screen Saver 3 12 Setting the System Clock 3 13 Checking the Hardware Options tray type 3 14 Setting the Eror Recovery Mode 3 15 Defining the Communications Options 3 16 Serial Ports 3 16 BCD Port Output 3 16 Accessing the Communication Screen 3 18 Selecting the Communication Option 3 19 Setting the Temperature of the Optional Peltier Accessory 3 20 Accessing the Peltier Accessory Screen 3 20 Chapter 4 Creating Methods and Sequences Defining the Active Method Samples Only 4 2 Procedure for Modifying the Default Active Method Samples Only 4 3 Defining the Active Method All Options Enabled 4 5 Adding Calibrants 4 6 Setting Up Timed Events 4 9 Setting Up a Derivatization 4 11 Setting Up a Dilution 4 13 Creating New Methods 4 18 Creating a New Method from within the Active Method 4 19 ii Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Creating a New Method by Recalling a Method from the Directory 4 21 Editing a Stored Method 4 24 Defining a Sequence 4 26 Rules for Defining a Sequence 4 26 Identifying the Methods to Include in a Sequence 4 26 Procedure for
182. utine Maintenance and Inspection 7 4 Viewing the Instrument Log 7 4 Replacing a Fuse 7 5 Locating and Removing the Power Input Module 7 5 Changing the Fuse s 7 6 Changing the Sample and Flush Syringes 7 8 Removing the Old Syringes 7 8 Loading the New Syringes 7 9 Replacing the Sample Needle 7 11 Changing the Transfer Tubing 7 15 Removing the Injector Valve Cover 7 15 Removing the Autosampler Cover 7 16 Replacing the Tubing 7 16 Flushing the System Replacing the Cover 7 18 Removing the Injector Valve 7 20 Removing the Injector Valve Cover 7 20 Disconnecting the Extemal Tubing Connections 7 20 Removing the Injector Valve Assembly 7 22 Dismantling the Injector Valve 7 24 Replacing the Rotor Seal and Stator Face Assembly 7 24 Reassembling the Injector Valve 7 26 Reinstalling the Injector Valve in the Autosampler 7 28 Changing the Sample Loop 7 30 Replacing the Tubing to the Column 7 31 Replacing the Tubing to the Pump 7 33 Replacing the Waste Tubes 7 35 Chapter 8 Troubleshooting Troubleshooting Table 1 Performance Problems 82 Troubleshooting Table 2 General Instrument Problems 8 8 Troubleshooting Table 3 Display Eror Messages 8 10 Appendix A Sample Trays Appendix B Changing the Performance Parameters Appendix C Serial Dilutions Appendix D Fixed Loop Mode Appendix E Operating Guidelines for Derivatizations and Dilutions 9 95 Rev C
183. utosampler This information can be used to help you set up a routine maintenance schedule S N 291N1234567 VALVE CYCLES 335 INJECTION CYCLES 998 DISPLAY HOURS 18 VALVE SET VALVE CYCLES TO ZERO ERR DISPLAY ERROR LOG av fe E Jose ere If you change the injector valve and need to reset the valve cycles press F1 3 EA The number of injection cycles is not resettable Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Viewing the Instrument Log Calibration and Diagnostics Viewing the Instrument Log continued S N 291N1234567 VALVE CYCLES 335 INJECTION CYCLES 998 DISPLAY HOURS 18 VALVE SET VALVE CYCLES TO ZERO ERR DISPLAY ERROR LOG Cd ee ES ee A 4 To view the error log press F8 EGG A screen similar to the following appears ERROR LOG 14 APR 1994 14 00 5 Refer to Table 6 1 for a list of the error codes and their descriptions a To clear the error log press F1 EEJ Table 6 1 Error Codes ero oep eror Desinion BRAM Battery RAM test SMTR Sample Motor TEMP Failure to control to set temper IVLD Injection valve load ature DUA1 DUART test internal IVIN Injection valve inject These occur only on start up Contact PerkinEImer Service Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Calibration and Diagnostics Notes Notes Artisan Technology Group
184. valve mounting screws under the valve a actuator arm Stainless Steel Needle Guide Part No N293 1054 Allen screws One of two valve mounting screws Figure 7 12 Top of the Injector Valve Assembly 3 Refer to Figure 7 13 With one hand grasp the injector valve just above the sample loop With the other hand use a phillips head screwdriver not supplied to loosen the valve mounting screws on top of the valve injector assembly The mounting screws are located directly in front of and behind the injector valve stem see Figure 7 12 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Removing the Injector Valve Maintenance Injector valve stem Injector valve Sample loop Figure 7 13 Front View of Injector Assembly 4 Before you remove the injector valve note that the valve stem points to the 9 00 position as shown here Valve stem points to 9 00 position lt _ __ When you replace the injector it must be replaced so that the valve stem points in this direction 5 Remove the valve mounting screws and store them in a safe place Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Maintenance Dismantling the Injector Valve Dismanting the Injecto
185. w Methods section in this chapter 2 The maximum number of methods comprising a sequence is 10 3 If you have previously defined a sequence you will be able to overwrite but not delete methods that you have stored and assigned to the sequence as long as you have not linked the sequence Once you link the sequence you cannot overwrite or delete any of the methods in the sequence Identifying the Methods to Include in a Sequence Before defining the sequence you need to view the methods stored in the Autosampler s Directory so that you can identify the numbers and names of the methods you want to include in the Sequence To view the methods follow this procedure on the facing page Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining a Sequence Creating Methods and Sequences Viewing the Methods in the Directory 2 MAIN SCREEN METHODO02 READY E E Pf sea oe A erty Press F6 SGI from the Main Screen to go to the Directory Screen DIRECTORY SCREEN METHODO02 STORED METHOD NAME LAST MODIFIED DEFAULT 02 FEB 1994 12 00 UTM TEST 02 FEB 1994 13 00 STORE STOR OR RECALL RCL A METHOD Ci EC ES ee Using the cursor arrow keys move down through the list of stored methods Write down the number and names of the methods you intend to use in the sequence Press the key to go back to the Main Screen Artisan Technology Group Quality Instrumentat
186. xcess sample volume in the fixed mode refer to Table D 1 for the rec ommended sample syringe and sample loop size combinations Sample Loop Size uL Sample Syringe Size uL 5 10 20 250 50 100 1000 200 2500 Table D 1 Sample Syringe vs Sample Loop Size Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix D Fixed Loop Mode Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Appendix E Operating Guidelines for Denvatizations and Dilutions When performing derivatizations dilutions or serial dilutions on the Series 200 LC Autosampler using the vial caps listed in this appendix use the default settings for the Per formance Parameters In addition for derivatizations dilutions or serial dilutions set the number of mix cycles to 2 when you set up the method For directions on reviewing and changing the Performance Parameters refer to Appendix B Changing the Performance Parameters For directions on setting up dilutions or derivatizations refer to Chapter 4 Creating Meth ods and Sequences F or setting up serial dilutions refer to Appendix C Serial Dilutions When using the following vial caps When using vial caps with resealing septa such as rubber or silicone ensure that the Vent Mode Performance Parameter is set to ON Examples of these types of
187. xt sample and perform sample preparation before the other modules pump and detector are ready Needle Level This determines how far the sample nee 10 1 99 dle goes into the sample vial The value corresponds to a percentage of the total distance from the shoulder top of the sample vial to the bottom of the sample holder For example 0 is at the bottom of the sample holder 99 is at the shoul der of the vial Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Configuring the Autosampler Reviewing the Default Performance Parameters Default Acceptable Parameter Description Value Value or Range 10 Vent Mode When this feature is enabled On a vol On Off On ume of air equal tothe volume of sample and buffer to be withdrawn from the vial is first injected intothe sample vial This prevents a vacuum from developing when multiple samples are withdrawn from the vial 11 Flush Volume The volume of flush liquid used for each 700 uL 0 2500 uL cycle during the pre and post injection flush operation 12 Speed of the Flush The speed at which the flush syringe Syringe picks up buffer from the buffer reservoir and ejects it through the sample needle 13 Preinjection Flush The number of flush cycles before sample Cycles is injected 14 Post injection Flush Thenumber of flush cycles after sampleis Cycles injected 15 End of Method The number of flush cycles at
188. y Instrumentation Guaranteed 888 88 SOURCE www artisantg com Defining the Active Method All Options Enabled Creating Methods and Sequences Setting Up a Derivatization This section describes how to set up a derivatization DERIV DILUT option enabled within a method NOTE To achieve the best performance use the default Performance Parameter settings To review or change the Performance Parameters refer to Appendix B Changing the Per formance Parameters Depending on the types of vial caps you are using you may need to change the Vent Mode parameter refer to Appendix E Operating Guidelines for Derivatizations and Dilutions METHOD SCREEN METHOD0O0 CHANGED FIRST LAST VOLUME REPLICATES TIME 1 4 10 2 5 0 ENTER FIRST VIAL NUMBER 1 225 M E TS SS a Press F4 to move to the Method Derivatization Screen METHOD DERIVATIZATION SCREEN METHOD00 CHANGED DERIV ENTER TOTAL NUMBER OF REAGENTS 0 5 0 Using the numeric keys on the keypad type in the number of reagents 2 is used in this example NOTE If you make a mistake entering numeric values in any of the fields on this screen press the key and retype the number Press the key and the Derivatization Screen changes to display the correct number of reagent columns in this example columns 1 and 2 The Sampl Vol field is highlighted METHOD00 CHANGED DERIV SAMPL VOL VIAL 2 VOLUME 0 MIX CYCLES 0 REACT TIME 0 o0 ENTER SAMPLE VOLUME 0 25

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