USB-MEA32-STIM4 Manual - Multi Channel Systems
Contents
1. 14 S Holding 14 Setting up an Experiment PHOT A Aiea N 14 SUCTION 2 488000 14 da Ap Mer Software Installation G Suction Port Gain Factor 9 ORIN ii de ode Ground 14 T Technical Specification Inverted Microscopes 9 Technical Support L Temperature Controller LTP Analyzer 5 Test Model Probe LTP Director 5 Test MEA32S12 Lx M Troubleshooting MC DataTool 5 U MC Rack 5 USB MC Stimulus Il 5 14 10 36 35 14 36 36 33 14 432. The USB MEA32 STIMA is a plug and play device The driver is automatically installed together with the MC Rack LTP Director program It is easier to install the software first and then connect the USB MEA32 STIMA to the data acquisition computer Installing MC Rack with the USB MEA32 STIM4 connected to the computer The Windows operating system detects new hardware when the USB MEA32 STIMA is connected to the computer if the program has not been installed beforehand Please make sure the device has power Simply cancel the Found New Hardware Wizard and proceed with the installation of the MC Rack program Connecting the USB MEA32 STIM4 to a computer with installed MC Rack If MC Rack and or LTP Director had already been installed on the computer before the hardware was connected the operating system needs to load the driver once After this procedure the USB MEA32 STIM4 will be automatically recognized by the operating system 1 Connect the USB MEA32 STIM4 to the USB port of the data acquisition computer 2 Switch the computer on The Found New Hardware Wizard is displayed 3 Choose the option No not this time and continue with Next 4 Choose the option Install the software automatically Recommended and continue with Next 5 The Wizard will automatically find the appropriate USB MEA32 STIM4 driver Continue with Next 6 The driver installation is finished For installation of MC Rack or LTP Director ple
3. C a Trigger 7 G C C 1 Cancel 30 5 2 4 Recording and Stimulation With these settings a Sync Out will automatically be generated for each programmed stimulation pulse and each of the four available triggers will start one stimulation channel and the corresponding Sync Out Please see the MC Stimulus ll manual for detailed description of Auto Sync and Trigger Settings function r T r T 00 00 00 000 00 00 00 001 t hh mm ss zzz 00 00 00 002 It is now possible to start and stop each channel and the corresponding Sync Out individually with the four start stop buttons for the four internal triggers An orange control LED indicates an active stimulation channel Sweep Er ul ja The regular Start Stop buttons will start and stop all channels Please remember downloading the stimulation pattern after each change with the Download or Download and Start buttons 15 Triggering on Sync Out in MC Rack The Sync Out channels 1 to 4 show up in MC Rack on the bits 0 to 3 of the digital channel To trigger on those use a Trigger Detector in MC Rack set on the digital channel To trigger on one Sync Out select the appropriate bit as shown below for Sync Out 1 To trigger on any Sync Out unmask bits 0 to 3 and set the condition to gt 0 Please see the screen shots below Sync Out 1 bit 0 Sync Out 2 bit 1 Sync Out 3 bit 2 Sync Out 4 bit 3 Channel D
4. The USB MEA32 STIMA4 is a plug and play device The driver is automatically installed together with the software program Set up the USB MEA32 STIMA4 amplifier Set up the 01 02 Connect the USB output connector to a free USB 2 0 port of the data proceeding computer It is not recommended to use an USB hub Please be aware that USB ports on the front of a PC are often connected to the main board via internal USB Hub If possible use a USB Port on the back Connect the USB MEA32 STIM4 via power supply unit to a power outlet of the same electrical system connected to the same ground earth wire as all other components of the setup for example the computer or shielding If necessary connect the system to an external ground Connect the PHO1 perfusion cannula to the 01 02 temperature controller s output channel D Sub9 socket with the provided cable Do not connect the PHO1 cable to the computer Start the MC Rack program and select the USB MEA32 STIM4 as the data source Please see the MC Rack help or manual for more details on how to define the data source Start MC Stimulus Il program for stimulation Please read the MC Stimulus help or manual for information Optionally Start LTP Director program Please read the LTP Director help or manual for information 17 USB MEA32 STIM4 Manual 4 4 Working with the USB MEA32 STIM4 Amplifier 4 4 1 Setting up the Amplifier For opening the lid of the amplifier
5. 60 1 39 Hw Rev B Analog 64 Digital 1 Data Format 16 bit Offset Correction Learn Offsets Offset Correction 10 Add a data display to you virtual rack by clicking the data display icon or by selecting it from the Edit menu The data display will open with the layout selected in the Data Source Setup Enter Amplifier Gain x Caution Amplifier gain is a hardware property that cannot be changed by software controls See the technical specifications for details or ask the manufacturer Enter the gain of the used amplifier Amplifier Gain fi 000 Default for MEA1060 Amplifier 1200 ME41060 BC Amplifier 1100 USB MEA256 1100 USB MEA32 STIM4 1000 Wireless Headstage 100 2100 Headstage 10 Cancel 11 Click the Start button to start the data acquisition No data is recorded You see the raw data streams of all 32 channels in the specific layout 12 You may have to adjust the position and span of the axes until you can clearly see the noise level You should see the baseline with a maximum noise level of 8 uV Be aware that the USB ME32 STIMA has wide filter bandwidth 0 5 Hz to kHz Therefore it is likely that you will pick up low frequency noise To avoid this use a virtual 10 Hz High Pass filter see MC Rack manual for details 24 Recording and Stimulation Operating more than one USB MEA32 STIM4 System with multiple instances of MC Rack Using USB ME
6. Device 4 3 1 Front Unit for pMEA with Suction Port The perspex insert in front of the main unit contains the frame for the pMEA on top and the suction port below A tube is connected to the small sealed compartment By this tube suction can be applied to the MEA from below either manually via syringe or via controlled vacuum pump CVP Holding Clamps To open the unit unlock the holding clamps on the left and on the right The upper part of the unit swings open 24999 9479 099 14 USB MEA32 STIM4 System Contact Pins of the Amplifier When the unit is closed the contact pins of the amplifier connect to the contact pads of the pMEA The contact pins are arranged in five rows with nine pins each Perfusion Cannula PHO1 The PHO1 is used for controlling the perfusion of the perfusion chamber on the pMEA The connected temperature controller TC01 02 keeps the temperature of the perfusion medium constant Ground Connectors Two ground connectors are available on the front panel of the USB MEA32 STIMA device A cable is attached to the which should be connected to one of the ground connectors A short cable with two small crocodile clamps is also provided to connect the suction cannula and the PHO1 and thereby also ground the cannula 15 USB MEA32 STIM4 Manual 4 3 2 Rear Panel USB The USB connector is used to transfer the amplified and digitized data from all data channels and the additional digit
7. STIM4 Important Safety Advice Operator s Obligations Guarantee and Liability Important Safety Advice Software Installation System requirements Recommended BIOS settings Driver Installation USB MEA32 STIM4 System The USB MEA32 STIM4 System Components 4 1 1 Filter Amplifier 4 1 2 Data Acquisition 4 1 3 Stimulus Generator 4 1 4 Perfusion 4 1 5 Suction Port Perforated MEA The USB MEA32 STIM4 Device 4 3 1 Front 4 3 2 Rear Panel 4 3 3 Connecting the USB MEA32 STIM4 Working with the USB MEA32 STIM4 Amplifier 4 4 1 Setting up the Amplifier 4 4 2 Perfusion 4 4 3 Pressure Control in the Suction Port 4 44 Setting up an Experiment N BWW y UI 10 10 10 11 11 14 14 16 17 18 18 20 21 22 USB MEA32 STIM4 Manual vi 5 1 5 2 5 3 6 1 6 2 6 3 6 4 7 1 7 2 7 3 7 4 7 5 7 6 Recording and Stimulation 23 MC Rack Data Acquisition and Analysis 23 MC Stimulus Stimulation Control 28 5 2 1 Device Setup 28 5 2 2 Selection of Stimulation Electrodes 29 5 2 3 Programming of Stimulation Pulses and Triggers30 5 2 4 Triggering on Sync Out in MC Rack 31 LTP Director Recording and Stimulation Software 32 Troubleshooting 33 Troubleshooting 33 Overall Noise 33 No Computer Connection No Recording Possible 34 MEA is defective 34 Appendix 35 Technical Support 35 Contact Information 35 Technical Specifications 36 Test Model Probe 38 Data Sheet 39 Scope of Delivery 40 Index 41 1 1 Int
8. band the gain is approximately 70 of the full gain Therefore you should use a bandwidth that is at a safe distance of the signals of interest Outside the pass band the gain decreases with the frequency and finally approaches zero For more information on gain and filters in general please refer to standard literature or contact your local retailer Raw data from up to 32 electrodes of a microelectrode array MEA is amplified by 32 channels of filter amplifiers that are built very small and compact using SMD Surface Mounted Devices technology The small sized MEA amplifier combines the interface to the pMEA probe with the signal filtering and the amplification of the signal The compact design reduces line pick up and keeps the noise level down The MEA sensor is placed directly into the small sized USB MEA32 STIM4 amplifier When the amplifier is closed the contact pins in the lid of the amplifier are pressed onto the MEA contact pads The very close location of the amplifier to the MEA sensor is very favorable concerning a high signal to noise ratio The amplifier is intended to be used with a binocular or an inverted microscope The USB MEA32 STIM4 amplifier is compatible with most standard microscopes If necessary you can use a Faraday cage or appropriate materials for example aluminum foil for shielding the amplifier The shielding should be connected to the amplifier s ground 4 1 2 4 1 3 4 1 4 USB MEA32 STIM4 Manua
9. there are sometimes hardware incompatibilities of the USB MEA32 STIM4 and computer components or that an inappropriate computer power supply may lead to artifact signals Please contact your local retailer for more information on recommended computer hardware Important You need to have installed the latest USB MEA32 STIM4 driver to operate the USB MEA32 STIMA which is automatically installed with MC Rack LTP Director The installation may be invalid if the USB MEA32 STIM4 does not respond Please contact Multi Channel Systems or your local retailer in this case Recommended BIOS settings Recommended operating system settings The following automatic services of the Windows operating system interfere with the data storage on the hard disk and can lead to severe performance limits in MC Rack These routines were designed for use on office computers but are not very useful for a data acquisition computer Turn off Screensaver Turn off Windows Indexing Service Turn off Windows System Restore Manual management of automatic Windows Update Power Options Power scheme Never turn off monitor hard disk and system standby It is also not recommended to run any applications in the background when using MC Rack Remove all applications from the Autostart folder Be careful when using a virus scanner These programs are known to disturb MC Rack and even data loss may occur 3 3 Software Installation Driver Installation
10. to the ground plugs of the system If using a include a metal part in the tubing to the and ground it Noisy equipment is too close by for example the binocular lamp Cover the pMEA with aluminum foil and connect the foil to the system ground Remove or shut down unneeded equipment Problem Low frequency noise Possible causes The USB MEA32 STIM4 has a wide filter bandwidth 0 5 Hz to 3 kHz Therefore it is likely that you will pick up low frequency noise Use a virtual 10 Hz High Pass filter please see MC Rack manual for details 33 6 3 6 4 USB MEA32 STIM4 Manual No Computer Connection No Recording Possible Problem You cannot establish a connection to the computer USB MEA32 STIM4 is not available in MC_Rack When loading a previously saved virtual rack file you will get an error message and the simulation mode will be started automatically Or you get an error message when starting the recording in MC_Rack after a successful computer connection Possible causes The supply power is not connected or there is a technical problem with the device Check the power source and the cable connections If this does not solve the problem contact your local retailer for support The USB port might not support USB 2 0 or might not be working Check the USB port Only high speed USB 2 0 ports can be used Try another USB 2 0 port You can establish a connection but get an error message when star
11. 0 C to 70 C 10 to 85 non condensing 235 x 135 x 55 mm with PHO1 on top 900 g 32 4096 16 0 5 Hz to 3 kHz 1000 typical 0 01 96 max 0 1 96 up to 50 kHz 6 4 MByte per second 16 Bit USB 2 0 High Speed 4 256 uA 13 bit 50 kHz 20 us PT 100 with 4 wire connection 0 1 C 200 Power supply unit MPU30 Input voltage Output voltage Max Power Rear Panel interface and connectors USB Ground Power supply Software Microsoft Windows operating system MC Rack program Data acquisition and analysis software MC DataTool program Data export MC Stimulus Il Stimulation software LTP Director Data acquisition analysis and stimulation software Appendix 90 to 264 VAC 47 to 63 Hz 11 13V 30 W USB 2 0 Speed cable type A mini B Common jack 4 mm banana plug Barrel connector 0 7 x 2 35 mm Windows 7 Vista or XP with NTFS English and German versions are supported Version 3 7 0 and higher Version 2 4 5 and higher ASCII txt binary file raw format Version 3 0 0 and higher Version 1 0 0 and higher Warning The USB MEA32STIM4 device may only be used together with microelectrode array pMEA32512 Lx from Multi Channel Systems MCS GmbH and only for the specified purpose Damage of the device and even fatal injuries can result from improper use Do not open the data acquisition box and do not change hardware configuration as it c
12. 1 E Config USB MEA32 Stim4 Ser 00015 Instance 1 In the main window of LTP Director Test Mode you see the first layout of the 2512 11 Select the stimulation electrodes S1 to 512 in the white field of the blue control window that mirrors the pMEA layout rotated by 90 degree The LTP Director can deliver two independent stimulation pathways to different electrodes One electrode can be selected for each pathway The electrode of stimulation pathway S1 is labeled with a small red square in the upper left corner of the electrode symbol in the electrode grid The electrode belonging to stimulation pathway S 2 is labeled in black Selected pulse parameters apply to both pathways To select an electrode for stimulation pathway S 1 just click on the electrode you want to select it will be marked in red To select an electrode for S 2 hold the Shift key and click on the electrode it will be marked in black To deselect an electrode click on the electrode symbol again or press Shift key and click again LTP Director does not allow to select more than one electrode for one pathway Please read the LTP Director manual or help for detailed information The pulse interval time between two pulses of one pathway can be set between 0 5 and 180 seconds This interval refers to one stimulation pathway When using two stimulation pathways pathway S 2 will be delivered in the middle of the pulse interval of pathway S1 So the time delay bet
13. A32 STIM4 Systems it is possible to operate up to four devices with one instance of MC Rack for each device on a single data acquisition computer Click Advanced Configuration in Edit menu to select the number of instances of MC Rack that you want to run simultaneously Please see the following screen shot Advanced Configuration Max Number of MC RackInstances 2 v Stop Data Loss Start the first instance of MC Rack Click Data Source Setup on the Edit menu All connected devices should become available in the Data Source with different serial numbers Select the device you want to control with the first instance of MC Rack via serial number Channel Layout x Data Source use y USB MEA32 5TIM4 S N 00004 y USB MEA32 STIM4 S N 00003 USB MEA32 STIM4 S N 00004 m Source Layout No of Channels 1 dimensional Total 32 C 2 dim MEA No Electrode Analog Configuration 1 32 0 Digital Input Ch Adjust the settings as described above Start the second instance of MC Rack In the Data Source select a device with a different serial number and repeat the process described above 25 USB MEA32 STIM4 Manual Once all MC_Rack instances are assigned to the respective devices you can operate each device independently The serial number of the connected device will be shown in MC_Rack main window SB MEA32 STIM4 5 00003 Rack C
14. MEA can be reused several times and is perfect for long time experiments up to several weeks and even months The electrode impedance ranges between 30 and 50 Tracks and contact pads are made of titanium nitride TiN the insulation material is polyimide respectively The electrodes are integrated into a thin polyimide foil This thin foil is fixed on a ceramic waver for mechanical stability In the middle of the waver under the electrode field there is a hole that makes it possible to access the electrode field from below The area around the electrodes is perforated to apply suction to the slice from below Please read chapter Working with the USB MEA32 STIM4 Amplifier The total area of the holes averages 0 8 mm the diameters of the holes varies from 20 to 90 um Because of the polyimide foil perforated MEAs are heat stable up to 50 C only 11 USB MEA32 STIM4 Manual Important Do not autoclave or sterilize pMEAs by heat This type of MEA is not heat stable and will be irreversibly damaged For cleaning rinse them with distilled water first then apply 1 Terg A Zyme solution Sigma for several hours Rinse the pMEA again with distilled water and dry them directly before use Sterilization with 70 ethanol is possible PMEA32S12 Layouts The following pictures show the four different pMEA layouts currently available each with twelve electrodes for stimulation and 32 electrodes for recording Please see chapter D
15. al channel to any connected data acquisition computer via High Speed USB 2 0 type A mini B cable Power In Connect the power supply unit This power supply powers the USB MEA32 STIMA4 main unit only The device needs 9 V and 1 3 A 11 7 W Ground If an additional ground connection is needed you can connect this plug with an external ground using a standard common jack 4 mm for the plug and a crocodile clip 16 4 3 3 USB MEA32 STIM4 System Connecting the USB MEA32 STIM4 USB MEA32 STIMA is a highly compact system with integrated amplification data acquisition and analog digital conversion Via USB High Speed 2 0 it is possible to transfer a digitally converted and amplified data stream of up to 32 electrode channels to any data acquisition computer Note Using a USB hub for connecting the USB MEA32 STIM4 to the computer is not recommended The system needs a broad bandwidth for the data transmission Recording might not be possible especially if a second device that sends or receives continuous data streams for example a web cam or USB speakers is connected to the same USB port Provide a power supply in the immediate vicinity of the installation site Place all devices on a stable and dry surface where the air can circulate freely and the devices are not exposed to direct sunlight Set up the computer Install the MC Rack LTP Director program from the installation volume if it is not already installed
16. an be normalized on baseline values and exported as relative ASCII values to a database Please check the system requirements before you install the software MCS cannot guarantee that the software works properly if these requirements are not fulfilled Please see the appropriate help or manual for more information It is recommended that you check the MCS web site for software updates on a regular basis The USB MEA32 STIMA is a plug and play device The driver is automatically installed together with the software It is easier to install the software first and then connect the USB MEA32 STIMA to the data acquisition computer Important Please make sure that you have full control over your computer as an administrator Otherwise it is possible that the installed hardware does not work properly 3 1 3 2 USB MEA32 STIM4 Manual System requirements Operating System One of the following Windows operating systems is required Windows 7 VISTA or XP English and German versions supported with the NT file system NTFS Other language versions may lead to software errors Hardware Not required for offline analysis or demo mode The data acquisition board USB MEA32 STIMA no USB MEA32 STIMA is present MC Rack LTP Director opens in a simulation mode A computer with low performance may lead to performance limits more often therefore MCS recommends an up to date computer preferably with two separate hard disks Please note that
17. ase refer to the respective manuals It is not recommended to run any applications in the background when using MC Rack Remove all applications from the Autostart folder Warning The operating system settings of the data acquisition computer were pre configured by MCS and should not be changed by the user Changing these settings can lead to program instabilities and data loss 4 1 4 1 1 USB MEA32 STIM4 System The USB MEA32 STIM4 System Components Filter Amplifier The filter amplifier combines a band pass filter and the signal amplification in one instrument The bandwidth of 0 5 Hz to 3000 Hz is suitable for a broad range of applications such as spikes and field potential recordings The digital filter of the MC_Rack program can be used to adjust the pass band and filter the raw data Please see the MC_Rack help or manual for more information For most LTP experiments it is recommended to use a digital 10 Hz high pass filter to avoid low frequency noise Please note that the gain factor of the filter amplifier 1000 is a fixed hardware property that cannot be changed by software controls Please also note that the ratio of the output signal to the input signal that is the gain is not a fixed parameter for the complete bandwidth The gain that was specified for the amplifier for example 1000 is not fully reached at the borders of the amplifier s pass band The general rule is that at the lower and upper limit of the frequency
18. ata Sheet in the Appendix for pin layout and for MC_Rack channel maps of the pMEA layouts 1 to 4 The layout pMEA32S12 L1 for example is specifically designed to fit the hippocampal structure The three rows of recording electrodes cover the CA1 area Cornu ammonis of the hippocampus The two rows of stimulation electrodes cross the Schaffer Collaterals The other layouts L2 to L4 are for more general use The ellipse indicates the perforated area L1 L2 L3 LA 12 USB MEA32 STIM4 System Perfusion The pMEA carrier is equipped with a glass ring The ring is 6 mm high and has an outer diameter of 24 mm The ring can be used as perfusion chamber The USB MEA32 STIM2 System provides a PHO1 perfusion cannula on top of the device For perfusion you need a peristaltic pump and for regulating the temperature of the perfusion medium a temperature controller 01 02 connected to the PHO1 Suction Through the perforation in the pMEA suction can be applied to the slice from below The suction keeps the slice in place and ensures a good electrode to tissue contact without the need for a weight The images show a hippocampal slice with and without suction being applied from below Suction applied Suction can be applied manually with a syringe or with a controlled vacuum pump also available from MCS Please see chapter Working with the USB MEA32 STIM4 Amplifier 13 USB MEA32 STIM4 Manual 4 3 The USB MEA32 STIM4
19. d with the powerful and easy to use MC Rack program from Multi Channel Systems MCS GmbH You can export the data in standard formats to other programs with the software MC DataTool Stimulus program controls the stimulation on up to four of the twelve stimulation electrodes at a time As a more convenient alternative for LTP long term potentiation experiments the new software package LTP Director and LTP Analyzer is available This software is able to control both recording and stimulation from one program and also has many useful features for LTP recordings The USB MEA32 STIMA is designed primarily to do recordings from acute slices Four different layouts of the perforated MEAs are available each with 32 recording and twelve separate stimulation electrodes The USB MEA32 STIM4 is not compatible with the squarish standard 60 channel MEAs The integrated filter amplifier supports 32 electrode channels The amplifier gain of 1000 is fixed The bandwidth of 0 5 Hz to 3 kHz is suitable for a broad range of applications such as spikes and field potential recordings The device can be used in combination with a binocular or an inverted microscope Acute slices can be kept in position and in good contact with the electrodes by applying suction through the perforated MEA from below 2 1 2 2 Important Safety Advice Operator s Obligations The operator is obliged to allow only persons to work on the device who are familiar wi
20. e Use MC_Rack LTP Director or your custom data acquisition program to record from the test model probe and to check the amplifier Please see chapter Test Model Probe in the Appendix The following screen shot shows a recording from a USB MEA32 STIM4 System with a test model probe and asampling rate of 40 kHz 1012 326 400 sec X4wis 1000 gt ms gt 00 10000 IV Peak Detection Reverse Display 3719 sweep H vasis 150 0 0 500 500 19 4 4 2 USB MEA32 STIM4 Manual Perfusion A PH01 perfusion cannula is already integrated on top of the USB MEA32 STIM4 System Connected to TCO 1 02 temperature controller it is possible to keep the temperature of the perfusion medium constant at the desired value There is no heating from below as with the regular MEA amplifiers TT ON ay louueu mn nus LO Hd To connect the perfusion cannula PHO1 please read PHO1 manual for detailed information Bath or test solutions can be applied at flow rates from 500 pl to 4 5 ml per minute The PHO1 can be connected to any tubing that fits to the perfusion inlet luer lock and outlet 1 mm OD stainless steel cannula A titanium wire inserted into the cannula results in a turbulent flow that ensures that the temperature of the liquid is constant across the flow stream For setting up a complete perfusion system a pump and a perfusion port for aspirating the liquid from the culture chamber are required Standard s
21. ect a temperature controller 01 02 to the heating element via D Sub 9 connector the heating element guarantees constant temperature conditions for the biological sample placed on the pMEA 32S12 Lx The 01 02 can be controlled manually or by software TCX Control Please see the TCX manual for more information Suction Port A suction port is integrated below the frame for the perforated MEA It is a small sealed compartment that is connected to a tube The red O ring for sealing this compartment is made of silicon It has an inner diameter of 7 mm and a thickness of 1 mm Silicon 70 7 x 1 By the tube suction can be applied to the MEA from below either manually via syringe or via controlled vacuum pump CVP Please see chapter Working with the USB MEA32 STIM4 Amplifier Perforated MEA pMEA32S 12 Lx For the USB MEA32 STIM4 System special perforated MEAs have been designed They are different in size but identical in function to the regular pMEAs One layout of the perforated MEAs designed for the USB MEA32 STIM4 System has been optimized for acute hippocampal slices The flat round electrodes are made of titanium nitride TiN with a diameter of 30 um for the recording electrodes and 50 um for the stimulation electrodes The stimulation electrodes can not be used for recording and vice versa The interelectrode distances vary from 150 to 200 um MEAs with titanium nitride electrodes are very stable Therefore the p
22. etups for MEA recording use a peristaltic pump or syringe pump and a standard metal cannula with beveled tip for aspiration The height of the aspiration needle can be adjusted with a setscrew The setscrew is the screw closest to the tip of the needle Make sure to ground both the PHO1 and the aspiration needle The is equipped with a standard platinum temperature sensor PT 100 The fluid temperature is controlled by the connected temperature controller 01 02 Please read PHO1 and TCX manual for more information 20 USB MEA32 STIM4 System 4 4 3 Pressure Control in the Suction Port Pressure Control via CVP The pressure control unit CVP controlled vacuum pump is a vacuum pump with a pressure sensor and a waste bottle A sensor measures the pressure in the compartment attached to the waste bottle and can regulate the suction to maintain a constant negative pressure With this unit it is possible to precisely control the suction applied to the slice and to keep the negative pressure stable during the whole recording period The accuracy of the pressure control is 0 1 mbar the maximum negative pressure is 200 mbar below atmospheric pressure A negative pressure of 10 to 30 mbar is enough to keep a slice in place on the electrodes Please read CVP manual for setting up the vacuum pump In case of 50 Hz noise include a metal part in the tubing to the CVP and ground it to one of the systems ground connectors It is also recomme
23. ftware options available to run an USB MEA32 STIM4 System Controlling the data recording and processing with MC Rack in combination with MC Stimulus for the stimulation or working with LTP Director The LTP Director controls the data acquisition and the stimulation as well Please read the respective manuals or helps for detailed information MC Rack The recording and analysis software MIC Rack can be used to work with the USB MEA32 STIM4 System Channel maps for all pMEA layouts are provided please see chapter Data Sheet in the Appendix MC Rack can be opened up to four times on one PC to control four connected units independently MC Stimulus II Selection of stimulation electrodes and programming of the current pulses is done with MC Stimulus Il Programming of the internal stimulator works in the same way as the programming of the regular STG stimulators from MCS LTP Director and LTP Analyzer The software package LTP Director and LTP Analyzer is designed to run standard LTP LTD experiments in acute hippocampal slices It is less flexible than MC Rack but includes the control of recording stimulation and electrode selection and an integrated documentation of the experiment in one program Furthermore it is possible to control an automated perfusion system The course of the experiment can be designed up in front and then runs automatically LTP Director is for recording and LTP Analyzer for offline analysis of the data Results c
24. g operation or maintenance of the device Unauthorized structural alterations to the device Unauthorized modifications to the system settings Inadequate monitoring of device components subject to wear Improperly executed and unauthorized repairs Unauthorized opening of the device or its components Catastrophic events due to the effect of foreign bodies or acts of God USB MEA32 STIM4 Manual Important Safety Advice Warning Make sure to read the following advice prior to installation or use of the device and the software If you do not fulfill all requirements stated below this may lead to malfunctions or breakage of connected hardware or even fatal injuries Warning Always obey the rules of local regulations and laws Only qualified personnel should be allowed to perform laboratory work Work according to good laboratory practice to obtain best results and to minimize risks The product has been built to the state of the art and in accordance with recognized safety engineering rules The device may only be used for its intended purpose be used when in a perfect condition Improper use could lead to serious even fatal injuries to the user or third parties and damage to the device itself or other material damage Warning The device and the software are not intended for medical uses and must not be used on humans Malfunctions which could impair safety should be rectified immediately High Voltage Electrical cords mus
25. g Pressure Control Connect the controlled vacuum pump CVP to the tube of the suction port Please read CVP manual for setting up the controlled vacuum pump Alternatively Connect a 1 ml syringe to the tube of the suction port For more information refer to the MEA Application Note Acute Hippocampal Slices on Perforated MEAs Service and Maintenance You should clean the contact pins of the amplifier carefully with a soft tissue and alcohol or isopropanol from time to time especially if you have problems with the noise level For handling and cleaning of the pMEA please refer to the pMEA data sheet or to MEA Cleaning Quick Guide on the MCS web site www multichannelsystems com Warning Spilled liquid can damage or even completely destroy the electronics of the USB MEA32 STIM4 amplifier Please be careful when setting up your perfusion system and when starting the perfusion Take care that the flow rates of the inlet and outlet flow match so that flooding of the amplifier is efficiently prevented Please be very careful when handling the amplifier or cleaning the device The pins can be damaged easily 22 Recording and Stimulation MC Rack Data Acquisition and Analysis Please refer to the MC Rack manual for more information Start MC Rack Click Data Source Setup on the Edit menu Select USB MEA32 STIMA Channel Layout x Data Source use JUSB MEA325TIM4 S N 00015 71 r Source Layout No of Cha
26. hannels Recorder Window A Display 1 HAA ChannelTool 1 multichan nel Display 2 ME systems Status started Lag Opening an existing Rack File with USB MEA32 STIM4 and multiple instances of MC Rack If you build up and saved a complicate rack in the first instance of MC Rack which you want to reuse in the second instance of MC Rack please do the following Start the second instance of MC Rack Click Open in File menu The dialog Open Rack Files appears Select the rack file you want to open Before the selected file will open in the second instance of MC Rack you have to change the data source device specification from the copied file That is why the dialog Channel Layout in Data Source Setup will automatically appear again Please choose the appropriate USB MEA32 STIM4 device via serial number assigned the second instance of MC_Rack Channel Layout x Data Source Mm MEA USB MEA32 5TIM4 S N 00003 y USB MEA32 STIM4 S N 00004 Source Layout No of Channels 1 dimensional Total 32 y 2 dim MEA Mo Electrode Analog Configuration 1 32 0 Digital Input Ch 26 Recording and Stimulation Now the reused rack file will be opened in the second instance of MC Rack The data source used for the rack is shown in the header and after the data source icon USB MEA32 STIM4_00004 rck Rack Data Source USB MEA32 STIM4 S N 00003
27. igital Data D1 y Channel Digital Data D1 y Mask 112 3 415 5 7 8 9 1411 14141414 OTT ETT 415 8 7 8 9 10 11 13131915 Bit FO 1 2 3 4 5 6 7 8 9 1411114141414 Bit 011121314151617 8 3101 12131419 C C 200 lt lt Dead Time m Im Dead Time 200 Te Ca Ps ll c Trigger only on Sync Out 1 Trigger on Sync Out 1 2 3 or 4 For more details on triggering on the digital channel please read the MC Rack manual 31 5 3 USB MEA32 STIM4 Manual LTP Director Recording and Stimulation Software Start LTP Director In menu Settings click Hardware tab Select Hardware Configuration USB MEA32 Stim4 and Device Serial Number USB MEA32 STIMA 00015 The amplifiers gain is fixed to factor 1000 Select the Sampling Frequency File Settings View Help El amp x p m sins y 1000 po Amplitude Measurement Data Acquistion gt m Select Electrodes for Experiment Select Asa Al Ena So Ais Test Pulse Pulse Shape neg pos Phase pos neg Phase Phase Duration 100 us Amplitude 100 p Stimulus Paradigm Single Pulse C Paired Pulse so v ms Pulse Intervat 10 s each PW resp es e Bug Bod pos Ped fo E E Pod Red Fed Ee Fed s Bri 4 Fed Es El E E E En Layout pMEA 32512 L
28. imulation The Select Device dialog appears Please select the USB MEA32 STIM4 device you want to connect to by the serial number Like MC Rack MC Stimulus Il can also be opened several times at once Open one instance of MC Stimulus Il for each USB MEA32 STIM4 device connected to the computer and assign each instance to one device All available stimulation devices are listed by their serial numbers Select Device Communication with STG STG 00004 00003 00004 5 2 2 Selection of Stimulation Electrodes Select the stimulation electrodes in main menu Edit Channel Select Select Stimulation Channels Assign the 4 stimulation channels to electrodes off sve iss Ste Chaneli rrr Channel Fr off se Esla la le Channel 3 mI E EM MM Chamel4 xj It is possible to assign stimulation channel 1 and 2 to stimulation electrodes S 1 to S6 It is not possible to select the same electrode for both channels Stimulation electrodes S 7 to S 12 have to be assigned to channel 3 and 4 If you do not need four channels for stimulation click check box channel off is programmed Warning Please switch off all unused stimulation channels even if no stimulation pulse 29 5 2 3 USB MEA32 STIM4 Manual Programming of Stimulation Pulses and Triggers For programming and downloading of stimulation pulses in MC Stimu
29. iny golden the TiN is gone and the electrode is irreversibly damaged Electrodes may be damaged without changing their visual appearance though If too many electrodes are defective use a new pMEA 34 7 1 7 2 Appendix Appendix Technical Support Please read the chapter Troubleshooting of the user manual first Most problems are caused by minor handling errors Contact your local retailer immediately if the cause of trouble remains unclear Please understand that information on your hardware and software configuration is necessary to analyze and finally solve the problem you encounter Please keep information on the following at hand Description of the error the error message text or any other useful information and of the context in which the error occurred Try to remember all steps you had performed immediately before the error occurred The more information on the actual situation you can provide the easier it is to track the problem The serial number of the device You will find it on the backside of the housing or in MC Rack Hardware tab The operating system and service pack number of the connected computer The hardware configuration microprocessor frequency main memory hard disk of the connected computer This information is especially important if you have modified the computer or installed new hard or software recently The version of the recording software On the Help menu click About to dis
30. l Data Acquisition Electrode raw data are acquired from the special designed perforated MEA and digitized by the analog digital converter board that is integrated into the main unit Recorded signals are converted in real time into digital data streams at sampling rates of up to 50 kHz per channel You will not miss even fast biological signals Data is transferred to the computer via High Speed USB 2 0 port Stimulus Generator The internal stimulator generates current controlled pulses only The maximum output current is 256 pA the resolution is 13 bit and the time resolution is 20 us Twelve stimulation electrodes can be connected via software control to the four channels of the internal stimulus generator Selection of the stimulation electrodes and programming of the stimulation pulses is done either with MC Stimulus or with the LTP Director Please read the respective manuals helps for more information Perfusion Perfusion Cannula PHO1 suo3s s ku A PHO1 perfusion cannula is already integrated on top of the USB MEA32 STIM4 System Connected to a TC01 02 temperature controller it is possible to keep the temperature of the perfusion medium constant at the desired value There is no heating element below the MEA as with the regular MEA amplifiers Bath or test solutions can be applied at flow rates from 500 ul to 4 5 ml per minute The PHO1 can be connected to any tubing that fit
31. lus II please see the respective manual The USB MEA32 STIMA device has four internal trigger outputs Sync Out 1 to 4 which be assigned to the four stimulation channels These four Sync Out channels will show up in MC Rack on bits O to 3 of the digital channel so it is possible in MC Rack to trigger on the exact time point of stimulation from each of the four internal stimulation channels Please use the Auto Sync Settings dialog from the Edit menu to assign one Sync Out to each stimulation channel Check if the Auto Sync function is activated in the Settings menu Pre and post time are only relevant for amplifiers with blanking function both can be set to 0 Auto Sync Settings Sync M Ch Ch Ch3 Ch Sync2 Chi Ch Ch3 Ch4 Sync Chi Ch4 4 Chi Ch3 v Ch 4 Pre Time D Post Time D us Cancel be able to start stop all four stimulation channels individually please assign the stimulation channels and the corresponding Sync Out to individual triggers in the Trigger Settings dialog in the Edit menu Trigger Settings Mode Cont Mode Standard Multi File C Extended Multifile E On Off Settings Chi Ch25 pCh33 pCh47 51 52 53 S4 rRepeat Trigger C C 2 1 T gger2 e C C C e C C 1 T gger3 C C C
32. multichannel systems USB MEA32 STIM4 Manual MEA Information in this document is subject to change without notice No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH While every precaution has been taken in the preparation of this document the publisher and the author assume no responsibility for errors or omissions or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document O 2012 Multi Channel Systems MCS GmbH All rights reserved Printed 04 05 2012 Multi Channel Systems MCS GmbH AspenhaustraBe 21 72770 Reutlingen Germany Fon 49 71 21 90 925 0 Fax 49 71 21 90 92 5 11 info multichannelsystems com www multichannelsystems com Microsoft and Windows are registered trademarks of Microsoft Corporation Products that are referred to in this document may be either trademarks and or registered trademarks of their respective holders and should be noted as such The publisher and the author make no claim to these trademark 1 1 1 2 2 1 2 2 2 3 3 1 3 2 3 3 4 2 4 3 4 4 Table of Contents Introduction About this Manual Welcome to the USB MEA32
33. nded to use a valve between the CVP and the system SH 11 Pressure Control via Syringe Suction can be applied manually with a syringe Connect a 1 ml syringe to the tube After mounting the slice gently suck in about 20 to 50 ul with the syringe It is better not to start with the syringe at position 0 ml better have 100 to 200 ul of air already in Do not apply more suction or the slice will be sucked into the holes Most likely the negative pressure will dissipate over time but usually the adhesion of the slice to the pMEA surface is good enough by then to keep it in place anyway 21 4 4 4 USB MEA32 STIM4 Manual Setting up an Experiment Open the lid of the amplifier Place the pMEA32S12 Lx chip inside the USB MEA32 STIM4 amplifier The pin layout is described in chapter Data Sheet in the Appendix Close the lid Grounding the bath The 2 5 is equipped with a big internal reference electrode which will be automatically connected to the systems ground when the amplifier is closed Connecting Perfusion Cannula and Temperature Controller For setting up a complete perfusion system a pump and a perfusion port for aspirating the liquid from the culture chamber are required Standard setups for MEA recording use a peristaltic pump or syringe pump The standard metal cannula with beveled tip for aspiration is fixed on top of the USB MEA32 STIMA device beside the PHO1 Connectin
34. nix 27 5 2 5 2 1 USB MEA32 STIM4 Manual MC Stimulus Stimulation Control The twelve stimulation electrodes can be connected via software control to the four channels of the internal stimulus generator The internal stimulator generates current controlled pulses only Selection of the stimulation electrodes and programming of the stimulation pulses is done either with MC Stimulus or with the LTP Director Please read the respective manuals helps for more information Device Setup Start MC Stimulus Il In Settings menu of the main menu toolbar select STG Device Setup The STG Settings dialog appears Click radio button MEA32 STIM4 Restart MC Stimulus II for the settings to take effect STG Settings x r Choose your Device C 5764002 C STG2004 C 5764004 C sTG2008 5164008 C STG3008 FA MEA32 STIM4 C MEA2100 Channel Configuration Analog Channels 4 7 Syncout Channels 4 Trigger Inputs 4 coca In STG menu of the main menu toolbar please click Select Device F Mc Stimulus II MC Stimi stm File Edit STG Batch Signal View Window Settings Help Start GE O CHER Connect Stop 5 Downlaad uc Doywnid and Start GErTH A Channel Signal Disable Autocalibration max Amp min Ampli Channe Voltage Current 28 Recording and St
35. nnels 1 dimensional Total 32 y 2 dim MEA Configuration Digital Input Ch A Amp MEA USB MEA32 STIM4 y 3251214 y Hippocampus pMEA 32512 L1 3251212 pMEA 32512 L3 pMEA 32512 L4 Refresh Cancel Select 1 Configuration in Source Layout The total number of channels is 32 with an additional digital input channel if necessary The Amp will automatically become USB MEA32 STIM4 Select your MEA type from the MEA drop down menu Click OK to close the Data Source Setup Usually this setup has to be done only once Add USB MEA32 STIMA as a data source to your virtual rack On the Edit menu click Add Data Display to add a raw data display to your virtual rack Click the Hardware tabbed page of the USB MEA32 STIM4 23 USB MEA32 STIM4 Manual 9 Enter the amplifier gain fix 1000 and the designated sampling frequency For field potential recordings in slices a sampling frequency of 10 or 20 kHz is ideal Rack1 Rack Data Source USB MEA32 STIM4 5 N 00015 E Recorder a USB MEA32 STIM4 S N 00015 Hardware Info Settings Signal Voltage Range Amplifier Gain Input Voltage Range of Data Acquisition Board Sampling Frequency Driver Version Device Number of Channels ADC Resolution 16 bit 4096 0 to 4096 0 py 1000 00 4096 0 to 4036 0 mv 7 20000 y Hz 3 10 S N 00015 Pw 1
36. ould lead to improper behavior of the system 37 USB MEA32 STIM4 Manual 7 4 Test Model Probe 38 Appendix 7 5 Data Sheet 39 USB MEA32 STIM4 Manual 7 6 Scope of Delivery 40 1 1 USB MEA32 STIM4 device Temperature Controller TCO1 Data acquisition computer USB 2 0 High Speed cable type A mini B Power supply unit with country specific power cord Data acquisition and analysis software MC Rack Version 3 6 7 and higher Stimulation software MC Stimulus Il Version 3 1 1 and higher Data export software MC DataTool Version 2 4 3 and higher OPTIONAL Software LTP Director and LTP Analyzer Data acquisition analysis and stimulation software USB MEA32 STIM4 System Manual USB MEA32 STIM4 System Data Sheet pMEA32S12 Lx Layout as specified O rings for sealing the suction port Silicon 70 7 x 1 mm 8 Index 41 USB MEA32 STIM4 Manual 42 11 10 A MEA Layout Analog Digital Converter 10 O B O Ring Bandwidth 9 P C Perforated MEA Contact Information 35 Perfusion Cannula D Pin Layout T pMEA 32512 Lx Data Acquisition 10 pMEA 32512 L2 Data Sheet 39 32512 1 3 32512 14 Power Faraday Cage 9 Filter Amplification 9 R Filter Amplifier 9 Rear Panel irst f ieiunia First functional Tests 18 2 2 00 0 Front Panel 14 USB istos tectus Contact Pins 14 Ground
37. play the software version Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site sales info User forum The Multi Channel Systems User Forum provides the opportunity for you to exchange your experience or thoughts with other users worldwide Mailing list If you have subscribed to the mailing list you will be automatically informed about new software releases upcoming events and other news on the product line You can subscribe to the list on the contact form of the MCS web site www multichannelsystems com 35 7 3 USB MEA32 STIM4 Manual Technical Specifications The USB MEA32 STIM4 is a 32 channel amplifier with integrated Analog Digital board converting analog signals to digital data streams in real time It is intended to directly contact to a 32 electrode Micro Electrode Array MEA General Characteristics Operating temperature Storage temperature Relative humidity Dimensions L x D x H Weight Amplifier Number of recording electrodes Input voltage range Data resolution Bandwidth Gain Cross talk channel to channel Data converter and USB interface Sampling frequency Maximal data rate Data resolution USB Version Stimulus Generator Number of stimulation channels Output current Resolution Maximum frequency Time resolution PHO1 perfusion cannula Temperature sensor type Accuracy Heating element impedance 36 10 C to 50 C
38. roduction About this Manual It is assumed that you already have a basic understanding of technical and software terms No special skills are required to read this manual If you are using the device for the first time please read the Important Safety Advice before installing the hardware and software where you will find important information about the installation and first steps The device and the software are part of an ongoing developmental process Please understand that the provided documentation is not always up to date The latest information can be found in the help Check also the MCS web site www multichannelsystems com for downloading up to date manuals and help files USB MEA32 STIM4 Manual 1 2 Welcome to the USB MEA32 STIM4 The USB MEA32 STIMA is a compact and portable stand alone solution for extracellular recordings with integrated amplifiers data acquisition and a four channel current stimulus generator Perfusion heating and the possibility to apply suction through perforated MEAs are also included It is a highly compact system The system acquires data from up to 32 electrode channels The digitized electrode data is transmitted to the connected computer via universal serial bus High Speed USB 2 0 Thus it is possible to use any computer as a data acquisition computer also a laptop Up to four systems can be controlled independently from a single computer Data is recorded graphed analyzed and reviewe
39. s to the perfusion inlet luer lock and outlet 1 mm OD stainless steel cannula A titanium wire inserted into the cannula results in a turbulent flow that ensures that the temperature of the liquid is constant across the flow stream For setting up a complete perfusion system a pump and a perfusion port for aspirating the liquid from the culture chamber are required Standard setups for MEA recording use a peristaltic pump or syringe pump and a standard metal cannula with beveled tip for aspiration The is equipped with a standard platinum temperature sensor PT 100 The fluid temperature is controlled by the connected temperature controller 1 02 To connect the perfusion cannula PHO1 please read PHO1 manual for detailed information A cable is attached to the PHO1 which should be connected to the system ground A short cable with two small crocodile clamps is provided to connect the suction cannula and the PHO1 and thereby also ground the cannula Warning The device must not get in contact with fluids Spilled liquid can damage or even completely destroy the electronics of the amplifier This is eminently important when using a perfusion system Take care that the flow rates of the inlet and outlet flow match so that flooding of the amplifier is efficiently prevented 10 4 1 5 4 2 USB MEA32 STIM4 System Temperature Controller 01 02 The USB MEA32 STIM4 features a PHO1 with a PT100 temperature sensor If you conn
40. t be properly laid and installed The length and quality of the cords must be in accordance with local provisions Only qualified technicians may work on the electrical system It is essential that the accident prevention regulations and those of the employers liability associations are observed Each time before starting up make sure that the power supply agrees with the specifications of the product Check the power cord for damage each time the site is changed Damaged power cords should be replaced immediately and may never be reused Check the leads for damage Damaged leads should be replaced immediately and may never be reused Do not try to insert anything sharp or metallic into the vents or the case Liquids may cause short circuits or other damage Always keep the device and the power cords dry Do not handle it with wet hands Requirements for the installation Make sure that the device is not exposed to direct sunlight Do not place anything on top of the device and do not place it on top of another heat producing device so that the air can circulate freely Warning The device must not get in contact with fluids Spilled liquid can damage or even completely destroy the electronics of the amplifier This is eminently important when using a perfusion system Take care that the flow rates of the inlet and outlet flow match so that flooding of the amplifier is efficiently prevented Software Installation There are two so
41. th the safety at work and accident prevention regulations and have been instructed how to use the device are professionally qualified or have specialist knowledge and training and have received instruction in the use of the device have read and understood the chapter on safety and the warning instructions in this manual and confirmed this with their signature It must be monitored at regular intervals that the operating personnel are working safely Personnel still undergoing training may only work on the device under the supervision of an experienced person Guarantee and Liability The general conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply The operator will receive these no later than on conclusion of the contract Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software It is up to the user to use good laboratory practice to establish the validity of his her findings Guarantee and liability claims in the event of injury or material damage are excluded when they are the result of one of the following Improper use of the device Improper installation commissioning operation or maintenance of the device Operating the device when the safety and protective devices are defective and or inoperable Non observance of the instructions in the manual with regard to transport storage installation commissionin
42. ting the recording in MC Rack The bandwidth of the USB port is not sufficient for recording This can be the case if the USB MEA32 STIMA is connected via USB hub and a second device that sends or receives continuous data streams for example a web cam or USB speakers is connected to the same USB port Connect the USB MEA32 STIMA device directly to a USB port not via hub MEA is defective Perforated MEAs wear out after multiple uses This is considered a normal behavior pMEAs are also easily damaged by mishandling for example if wrong cleaning solutions or too severe cleaning methods are used or if the recording area is touched If you observe a bad long term performance of pMEAs consider a more careful handling Also do not apply too high too long stimuli This might lead to electrolysis and destruction of the electrodes Problem The noise level on individual electrodes is significantly higher than expected or you see artifact signals In the following example electrode number 13 shows a high noise level 326 400 sec 1000 p ms oo 10000 Peak Detection Reverse Display 371st sweep 4 1 YAris 50 gt 50 0 500 Possible causes The contact pads are contaminated Clean the contact pads carefully with a swab or a soft tissue and pure 100 96 alcohol The contact pads or the electrodes are irreversibly damaged You could have a look at the electrodes under a microscope If they appear sh
43. unlock the holding clamps on the left and on the right side The upper part of the unit swings open to enter the pMEA Now you can insert a test model probe or a perforated MEA into the frame Below the place for the pMEA is a small sealed compartment that is connected to a tube By this tube suction can be applied to the MEA from below Be sure that the red O Ring is in place This O Ring Silicon 70 7 x 1 mm seals the suction compartment to the pMEA 18 USB MEA32 STIM4 System Place the Test MEA32S12 Lx test model probe inside Replace the upper unit and close the holding clamps carefully First Functional Tests Each MEA amplifier has been thoroughly tested at the factory site before delivery However you may want to perform some tests yourself before you begin your experiment to exclude any damage that might have occurred during transportation Some of the tests will also help you to get to know the basic functions of the hard and software like a short tutorial It will take only a few minutes time and can save time and trouble in the long run Multi Channel Systems recommends running these tests after the setup of your system before you start your real experiments General Performance Noise Level Please use the provided test model probe Test MEA32S12 Lx to test the noise level of the amplifier immediately after installation It simulates a MEA with a resistor of 220 and a 1 nF capacitor between bath and electrod
44. ween a stimulus on pathway S 1 and S 2 will be half of the selected pulse interval Example Selected a pulse interval of 1 min and two pathways There will be one stimulus every 30 s alternately on pathway one and two Please see LTP Director manual for details 32 6 1 6 2 Troubleshooting Troubleshooting The following hints are provided to solve special problems that have been reported by users Most problems occur seldom and only under specific circumstances Please check the mentioned possible causes carefully when you have any trouble with the product In most cases it is only a minor problem that can be easily avoided or solved If the problem persists please contact your local retailer The highly qualified staff will be glad to help you Please inform your local retailer as well if other problems that are not mentioned in this documentation occur even if you have solved the problem on your own This helps other users and it helps MCS to optimize the instrument and the documentation Please pay attention to the safety and service information in the separate manuals of the related products and in the software help Multi Channel Systems has put all effort into making the product fully stable and reliable but like all high performance products it has to be handled with care Overall Noise Problem High frequency noise 50 Hz Possible causes Perfusion is not properly grounded Ground PHO1 and aspiration needle
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