µFlow - User Manual - Bioprocess Control
Contents
1. 4000 3500 3000 S 0 c c TS T dein om gh ditm eei ro i e pom iie ee eI pedes vs p pi LE eh Lm nc rue Lope i sat nn prid iri FERAM Ut Flow Nml hour 1000 00 00 04 00 08 00 12 00 16 00 20 00 00 00 04 00 08 00 12 00 16 00 20 00 00 00 05 24 08 24 08 24 06 24 08 244 08 25 08 25 08 25 08 5 08 25 08 25 08 26 Time 16 00 20 00 08 23 08 23 08 24 ly Copyright 2006 2013 Bioprocess Control Sweden AB All rights reserved bloprccess ONTROL 4 PRE COMMISSIONING The following items are not included in the delivered uFlow however they will may be required to operate the flow meter Data acquisition system available from BPC Additional wall socket adapters plugs contacts as the ones supplied are according to European US or UK standards depending on original purchase order If the customer has a gas chromatograph and is interested in off line biogas composition analysis gas sampling units can be ordered separately from BPC 5 QUALITY RULES AND RECOMMENDATIONS The product guarantee provided corresponds to the guarantee stipulated on the confirmed product order form and shipping documentation For high accuracy measurements the uFlow should not be exposed to mechanical vibrations and or high frequency radio transmissions and should be placed on a level and stable surface Only the parts delivered with the product can be used with the system in order to guarantee the quality and2. In the anaerobic biodegradation process gas flow rate represents a key parameter for evaluating the performance of a process In laboratory scale experiments the bioreactor volume ranges from 1 to 50 1 with a low gas production especially during the start up period often within several hundreds millilitres per hour Measuring such a low volume of gas accurately is not an easy task using commercially available gas flow meters since they do not measure such low flow rates A gas flow meter for laboratory scale reactors has higher demands compared with a meter adapted for a large scale anaerobic reactor This includes no gas losses stable calibration through time and higher sampling rates and ultimately more reliable gas measurements The uFlow meets all the needs of the most demanding biogas biofuel labs and user applications 2 1 Conventional Gas Flow Meters Conventional gas flow meters 1 utilise techniques e g thermal Coriolis mass ultrasonic turbine vortex shedding etc that measure parameters such as velocity or mass of the flowing stream to indirectly determine the volumetric flow or 11 measure the volume of the gas directly by liquid displacement Although use of the liquid displacement technique is simple and straightforward errors associated with volume determination still exist when the investigated gas is soluble in the displaceable liquid and the barrier solution is in direct and continuous contact with the atmosphere M
3. 1 1 Apache Software Foundation 9 Qualcomm Atheros Bluetooth Suite 64 Qualcomm Atheros A Qualcomm Atheros Driver Installation Program Qualcomm Atheros S Realtek Card Reader Realtek Semiconductor Corp x Realtek Ethernet Controller All In One Windows Driver Realtek X Realtek High Definition Audio Driver Realtek Semiconductor Corp AM Synaptics Pointing Device Driver Synaptics Incorporated uFlow DVel AB s UniversalDataLogger DVel AB Windows Driver Package C Technologies AB CPen C Technologies AB I Windows Essentials 2012 Microsoft Corporation v Installed On Size 10 09 2014 05 06 2014 05 06 2014 06 06 2014 06 06 2014 05 03 2015 03 10 2014 10 09 2014 05 06 2014 05 06 2014 05 06 2014 05 06 2014 05 06 2014 05 06 2014 05 03 2015 05 03 2015 30 01 2015 07 05 2014 DVel AB Product version 1 0 63 Supportlink http www dvel se e Help link http www dvel se Update information http www dvel se 33 Search Progra JO Version 10 1 MB 9 0 30729 6161 13 6 MB 10 0 30319 9 89 MB 10 0 30319 204MB 11 0 50727 1 173MB 11 0 50727 1 666 KB 1 0 30 338MB 4 11 9775 5 16MB 8 0 1 232 10 0 6 3 9600 29075 8 24 1218 2013 6 0 1 7164 464MB 18 04 0 6 32MB 1 0 63 6 30MB 1 0 12 02 22 2010 3 0 0 2 164 3508 0205 Size 6 32 MB biopr 2 if D oUn Un 10 2 uFlow Data Logger Software User Guide HOME Available information User Manual Specification Product S
4. Nml h in the generated report Right clicking in the graph area selected commands Clear Graph will just clear the curves in the graph view but all data that has been recorded will be kept The graph can be reloaded by pressing the Load Data button in the Load Data window It may take a little longer for the data to show up in the Graph window than it does in the Load Data window AutoScale X Automatically scales the x axis to contain all data AutoScale Y Automatically scales the y axis to contain all data Export A picture of the current view of the graph can be exported as a picture excel file or added to the clipboard To export the experiment data see the Load Data page guide 4 SYSTEM bIop System Information General SW Version DAQ Hardware Daq Type Serial Number IP address Contact Support E mail Info E mail Sees bloprce ONT Usb 19BEEEB 0 0 0 0 support bioprocesscontrol com info bioprocesscontrol com Ab Copyright 2006 2015 Bioprocess Control Sweden AB All rights reserved This section shows the System page The page contains general information about the software Data Logger hardware i e serial number IP address and contact details for troubleshooting and support 42
5. files and for the National Instrument products files that are part of the software 22 bioproc 5 Click next Destination Directory Select the primary installation directory All software will be installed in the following locations To install software into a different location click the Browse button and select another directory Directory for MyFlowDAQ Directory for National Instruments products 23 bloprccess ONT 6 When the installation summary shows up start the installation by clicking Next Start Installation Review the following summary before continuing Adding or Changing MyFlowDAQ Files Click the Next button to begin installation Click the Back button to change the installation settings 24 7 A window will show the installation progress Overall Progress 23 Complete biop Copying new files lt 4 Back 25 Next gt gt bloprcocess ONT 8 A message will follow when the installation is completed Click Next to finalise Installation Complete lt lt Back Frish 9 When the installation has been confirmed as successful restart the computer 10 Start the second part of the installation by opening the folder called Flow Installers Home Share View 09 X Cut TE X amp g Open HH Select all Copy path p EJ 2 Edit 59 Select none Copy Paste Move Copy Delete Rename New Properties A x History fa Pasteshortcut toy t
6. flow rate duration of the experiment and version of the software 3 Port for filling water 9 Adjustable foot 4 Inlet for gas flow 10 Button for instant pressure and temperature display press for less than 5 seconds and for resetting the timer press for more than 5 seconds 5 Maximum filling level indicator 11a Power input 6 Minimum filling level indicator 11b Standard signal output for automatic data acquisition 10 bloprcocess ONTROL 7 2 uFlow Gas Meter with Side Power and Analogue Output Port o Aa o volume Nml flow rate Nmi h ean 1 Outlet for gas flow 2 Level gauge 3 Port for filling water 4 Inlet for gas flow 5 Maximum filling level indicator 6 Minimum filling level indicator 1 time min 3 4 bioprocess ONTROL uFlow 11 version reset 9 10 7 Glass window for visual inspection of the flow cell movement 8 Liquid Crystal Display LCD for real time visualisation of normalised gas volume amp flow rate duration of the experiment and version of the software 9 Adjustable foot 10 Button for instant pressure and temperature display press for less than 5 seconds and for resetting the timer press for more than 5 seconds 11 Combined power input and analogue output port bloprcocess ONTROL Angle view of the uFlow meter with the combined power input and analogue output port on the
7. measurements Glass window for visualisation of flow cell movement Low maintenance requirements Pre calibration to ensure measurement accuracy 13 bloprccess ONTROL 7 5 Data Logger System Technical Characteristics The PC based Data Logger system for uFlow consists of a hardware module controlled by an easy to use software program DATA LOGGER HARDWARE The hardware module has 8 plug amp play terminal connectors that provide connections for 8 analog input channels Each channel has an AI plug amp play terminal to which you can connect a current signal The module has a common terminal COM that is internally connected to the isolated ground reference of the module The main characteristics of the hardware module are presented below e 8 analogue current input channels e 4to 20 mA programmable input ranges e Universal Data Logger with Ethernet interface 16 bit resolution 200 kS s aggregate sampling rate e uFlow Data Logger with USB interface 12 bit resolution 10 kS s sampling rate e Indoor application only e Power adapter input 100 240 V 50 60 Hz output 24 V DC 0 5 A or higher DATA LOGGER SOFTWARE The Data Logger software is needed in order for the Data Logger hardware to work with a PC It is delivered on a USB card together with the Data Logger hardware Please notice that the Data Logger software is delivered with a license control and can only work together with the Data Logger hardware delivered at the same t
8. side 7 3 Charger for the uFlow Gas Flow Meter The power input port is located at different positions on the two versions of the uFlow meters The charger is inserted at 11b on the front of the uFlow meter described in chapter 7 1 and at 11 on the side on the uFlow meter described in chapter 7 2 12 bloprccess ONTROL 7 4 uFlow Gas Flow Meter Technical Characteristics GENERAL Measuring resolution 10 ml Measuring range 20 to 4000 ml h Signal output for flow rate 4 20 mA Repeatability 196 Working principle liquid displacement amp buoyancy Built in sensors pressure and temperature LCD display normalised gas flow NmL h volume Nml and time min Materials high quality aluminium Dimension 19x14x6 cm Weight 0 180 kg Power supply input 100 240 V 50 60 Hz output 12 V DC 0 5 A Usage indoor applications for inert and slightly aggressive gases FEATURES Large linear detection range up to 4000 ml h which fits perfectly with most of lab and small pilot scale applications Embedded timer and reset button for easy logging of accumulated gas volume over a defined period of time Automatic gas flow and volume normalisation with real time temperature and pressure compensation which allow for gas measurement standardisation Standard analogue current signal output 4 20 mA for automatic data acquisition Adjustable foot and horizontal gauge to ensure the correct installation position for high precision
9. to a power outlet If the uFlow Gas Meter with the analogue output port on the front is used as a stand alone unit the green terminator plug must be inserted in the analogue output port 11b If the unit will be connected to a Data Logger system connect the power cable to either the power input 11a and the analogue output cable to the port on the front 11b or to the combined power and analogue output port on the side 11 again depending on which version is used When a Data Logger System is used make sure that the end of the analogue output cable marked with blue or grey for same versions 1s always connected to the Data Logger and not to the uFlow Gas Meter 15 bloprccess ONTROL INSTALLATION OF THE DATA LOGGER HARDWARE a Connect the Data Logger to the computer The Data Logger in the left picture below uses an USB interface and the Universal Data Logger in the right picture below uses an Ethernet cable The pictures show the back of the devices b It is very important that the blue or grey for some versions end of the analogue signal cable is used when inserting the cable into the port on the Data Logger Important If the Universal Data Logger is used please refer to the Universal Data Logger Operation and Maintenance Manual before connecting the signal cables The Universal Data Logger has more options for measuring parameters and signal processing types The pictures below show the cable ports on the
10. up 6 alternatives to zooming in out and changing the scales of the graph B Tr E ae A J one 1 The top left icon allows the user to resize the graph window to a smaller subset of the visible display by first clicking on the icon and then by clicking at a suitable point in the graph and then while still holding down the mouse button creating a square shape The graph window will then zoom in to display the data contained within the square shape 2 The top middle icon allows the user to resize the graph window in a similar way to the first option but with a focus on the x axis The y axis will automatically adjust to an appropriate value 3 The top right icon allows the user to resize the graph window in a similar way to the first option but with a focus on the y axis The x axis will automatically adjust to an appropriate value 4 The bottom left icon allows the user to resize the graph window to display all available data To do this click once on the icon 5 The bottom middle icon allows the user to zoom in on the graph by first clicking on the icon and then clicking in the graph window 6 The bottom right icon allows the user to zoom out on the graph by first clicking on the icon and then by clicking in the graph window 38 bloprccess ONTROL Right clicking in the graph area selected commands Clear graph will just clear the curves in the graph view but all data that has been recorded will be kept The grap
11. 0 16 The green status bars show the progress Overall Progress 25 Complete biop lt lt Back 31 Next gt gt 17 Click Finish to complete the installation Installation Complete The installer has finished updating your system 18 When the software installation has finished use the power adaptor to connect the Data Logger hardware to a power outlet Connect the Data Logger to a computer by the USB cable 22 bi O roCESS C oO T Z 19 The Data Logger software can be uninstalled in the Windows Control Panel The National Instruments files need to be uninstalled separately s m B gt Control Panel Programs Programs and Features Control Panel Home Uninstall or change a program View installed updates To uninstall a program select it from the list and then click Uninstall Change or Repair iy Turn Windows features on or zx Organise Uninstall Name Publisher ln Microsoft Visual C 2008 Redistributable x86 9 0 3 Microsoft Corporation E Microsoft Visual C 2010 x64 Redistributable 10 0 Microsoft Corporation E Microsoft Visual C 2010 x86 Redistributable 10 0 Microsoft Corporation g Microsoft Visual C 2012 Redistributable x64 11 0 Microsoft Corporation 29 Microsoft Visual C 2012 Redistributable x86 11 0 Microsoft Corporation E MyFlowDAQ DVel AB National Instruments Software National Instruments 5 OpenOffice 4
12. 21 16 02 02 2015 21 16 02 02 2015 21 17 Rex Be Delete Rename Move toy toy Copy Y Organise New folder New Properties PAES Rib History Open gt t d uFlowData Logger MyFlowDAQ Additional Installers Volume i Videos iip Windows C cx RECOVERY D ex USB DISK F amp fseventsd Spotlight V10C Ai Trashes d SW IF screenst Je SW installation i Netunel 5 items Name n bin license J supportfiles 1 item selected 1 32 MB A 21 Date modified 02 02 2015 22 17 02 02 2015 22 17 02 02 2015 22 17 14 08 2014 14 20 06 06 2014 09 08 14 08 2014 14 20 J cd Open A Edit Properti xii S Pa History Open g8 Select all Select v A Type File folder File folder File folder File folder Open fH Select all Edit Select e Type File folder File folder File folder ID File Application Configuration sett 88 Select none fe Invert selection Size 88 Select none Size Search MyFlo D 0 BE Invert selection Search Volume JO bloprccess ROL 4 When the setup exe file is opened installation will be initialised and a green bar will show the progress MyFlowDAQ Additional Installers This installer will install the additional resources needed for the MyFlowDAQ software to run Please wait while the installer initializes Select a destination directory for the MyFlowData Logger program
13. 9 7 p uFlow Data Logger uFlow Installers Volume v A Search Volume p E Videos A Name Date modified Type Size iia Windows C B tin cx RECOVERY D B mee e USB DISK F ffseventsd Spotlight V10C p Trashes d SW_IF_screenst e SW installation 13 02 2015 10 24 File folder 04 02 2015 18 24 File folder supportfiles 13 02 2015 10 24 File folder 10 02 2015 22 04 ID File 06 06 2014 09 08 Application 10 02 2015 22 04 Configuration sett i Nene 6items 1 item selected 1 32 MB ZI bloprcocess ONT 13 The installation is started and a green status bar shows the progress uFlow Installer This installer will update the uFlow software Additional resources needed for this software will NOT be installed a separate installer is needed for that Please wait while the installer initializes 28 bioproc 14 Select a destination directory for the files Destination Directory Select the primary installation directory All software will be installed in the following locations To install software into a different location click the Browse button and select another directory Directory for uFlow 29 bioproc 15 Start the installation by clicking Next Start Installation Review the following summary before continuing Adding or Changing uFlow Files Click the Next button to begin installation Click the Back button to change the installation settings 3
14. Stop logging button there are checkboxes for all 8 channels They can be used to hide and select which channels that should show up in the graph Check the box to select that channel and uncheck it to hide it Use the grey scrolling bar to find Channel 7 and 8 The graph shows the current flow rates for the selected channels The values in the graph are updated every 15 min after starting the logging and are normalised to 1 0 atm and 0 C Stop logging To stop data logging press the button Stop logging The button will turn green again and the system will stop recording any data Stopping the data logging will not delete anything it will only stop recording data until the Start logging button is pressed All logged data will be stored in the database files 3l bloprcocess ONTROL Scrolling and zooming in the graph plot By right clicking in the graph plot a menu will show up Choose Visible Items gt Graph palette to find a bar with 3 buttons where some zooming and scrolling tools will be found HAR The button with the hand icon can be used to move the graph in any direction A left mouse click that is held down while moving the mouse will move the graph according to the mouse movement A right mouse click that is held down will start scrolling the graph when the cursor reaches any edge of the graph to reveal more of the graph in that direction The button with a magnifying glass with a plus sign opens
15. as will leave the uFlow at the gas flow outlet 1 The off gas can be collected in a gas bag 1f the measurement of the gas composition is of interest or it can be linked directly to a fume hood through a gas tube Make sure the gas flow is within the measurement range of the flow meter Connect the power supply 12 V DC 0 5 A to the gas meter and to a 100 240 V 50 60 Hz standard power socket The uFlow is ready for operation 17 bloprccess ONTROL MOVING OF THE FILLED UNIT a Care should be taken if the uFlow has to be moved after having been filled with packing liquid In this case transportation should be done in vertical position in order to avoid packing liquid from exiting the unit 8 2 Start up At this stage it is assumed that the uFlow is already installed according to section 8 1 the gas source is connected to the inlet port of the gas flow and the power supply also is connected MEASURING DEVICE a Check the instant pressure and temperature by pressing the reset button for approximately 2 seconds b Reset the timer just before starting a new experiment This can be done by pressing the reset button 10 for more than 5 seconds or by disconnecting the power for the same interval of time c Check that the accumulated gas volume and the instant gas flow rate at the beginning of the experiment are displayed as 0 Nml and 0 Nml h respectively 8 3 Monitoring MEASURING DEVICE a Periodically check that the water l
16. e Em Testcht Testch2 V T Channel 4 N Channel 5 L V Channel v Channel ee ee eee E rei Flow Nml hour 1800 1750 1700 1650 1600 1550 1500 1450 20 44 20 45 20 45 20 46 20 47 20 48 20 49 20 50 20 50 20 51 20 52 20 53 20 54 20 55 20 55 20 56 20 57 20 58 20 59 21 00 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 01 26 Time lt Copyright 2006 2015 Bioprocess Control Sweden AB All rights reserved In the Load Data page it is possible to generate a report Load Data To start loading the data choose the period of interest 1 e start and stop times and then press the button Load Data The data for the selected time period will show up in the graph 40 bloprcocess ONTROL Generate Report By pressing the button Generate report a pop up window will be displayed with the following message The data load in the graph is at an interval of 15 minutes Choose at which interval you would like to save your report 15 minutes hour or daily After selecting the unit for the time e g quarter of hour hour or day and pressing OK the data will be saved into a csv comma separated values file The file can be opened and viewed through a spreadsheet program such as Microsoft Excel Open Office Calc or Google Docs The flow rate for the selected flow cells is presented in
17. e tops E ORE aeee 7 5 QUALITY RULES AND RECOMMENDATIONS eere 7 0 SAFETY CONSIDERATIONS pyrenees 8 7 EQUIPMENT DESCRIPTION DESIGN FUNCTION eeeerrnnnnnn 9 7 1 uFlow Gas Meter with Front Power and Analogue Output Port 10 7 2 uFlow Gas Meter with Side Power and Analogue Output Port ssssss 11 To Chareertor the i Flow Gas Flow Metelica E 12 7 4 uFlow Gas Flow Meter Technical Characteristics eeeeeesssssseeeeeeeeeeeeeennenen 13 7 5 Data Logger System Technical Characteristics cccccccecsssscccceccceeesseseececeeeeeaaeseeeeeeees 14 o OPERATION 5e outil eate qttod ia e tem natn ud qe temi 15 Sel ICE Ret NO RR TERES ETUERTEUTU REPRE EE 15 52 SEED rere meagre a re ge ere etant ete Luo ee ae ht ete ee 18 So EM Pere 18 O24 Endt ODerdtiofb su ppc rt ai a a A ions 19 9 MAINTENANCE tp ee p EE T EMI I 19 10 nFEOW SOETWARLD ipeo veniet oue suu tact sos p sagesuotuub senes sue vds taco sS gu P escun sub Feed stes 20 10 1 Installation of the uFlow Data Logger Software Windows eeeeessssss 20 10 2 Flow Data Logger Software User G de ceuroen eiei i RN 34 bloprccess ONTROL 1 PREFACE Bioprocess Control Sweden AB BPC is a privately held company and market leader in the area of advanced control technologies for the comm
18. e transport company documents the incident d Inform the seller of the incident 3 1 wFlow Gas Meter Box Content See also Chapter 7 Equipment Description for pictures of included parts 1 flow meter 1 power adapter input 100 240 V 50 60 Hz output 12 V DC 0 5 A manual 3 2 Optional Equipment For automatic data logging of gas flow rate BPC provides two different Data Logger systems together with the software for Microsoft Windows 1 Data Logger with USB interface biopr 2 if D oUn Un 2 Universal Data Logger with Ethernet interface The hardware module includes eight analog current input channels for high performance control and monitoring applications The system features analogue current input ranges of 4 to 20 mA To protect against signal transients the module includes a channel to earth ground double isolation barrier for safety and noise immunity When deciding between a USB and Ethernet interface it s important to weight the advantages and disadvantages of both When it comes to the Data Logger unit with a USB interface the most obvious benefit 1s cost In addition the USB instrument is easier to install and most PCs have plenty of USB ports to choose from On the downside the Data Logger with USB interface can only be accessed over a relatively short distance In addition the USB connection is not really designed for reliable data communication over a long period of time While slight
19. ercial biogas industry providing technologies and services that support the efficient design and operation of biogas plants amp processes The company was founded in 2006 bringing to market more than 15 years of industry leading research in the area of instrumentation control and automation of anaerobic digestion processes At the end of 2009 BPC launched the Automatic Methane Potential Test System I AMPTS I a revolutionary product in the area of on site lab equipment for methane potential analysis The number of users of AMPTS I grew quickly and today there are users in more than 40 countries located 1n the Americas EMEA and Asia Pacific regions In April 2011 an updated version of the AMPTS was made commercially available and at the end of 2012 the BioReactor Simulator BRS was introduced to the market As of January 2013 a new instrument uFlow for measuring ultra low gas flows with high precision has been offered to the market The u Flow is a flow meter which has been designed for on line real time monitoring of gases over a wide detection range and for most indoor laboratory scale applications Suitable applications include biogas process studies ethanol fermentation dark fermentation for bio hydrogen and leak rate detection bloprccess ROL 2 GENERAL INFORMATION Anaerobic biodegradation processes are gaining in interest among industry and local governments due to their ability to produce energy and reduce sludge yields
20. etails DETAILED GUIDE FOR EACH PAGE In each page it is possible to find additional menus by right clicking in different areas Not all the functions shown in these menus are activated in this application and only the ones that are useful for experiments with the uFlow Gas Meter are explained here 35 SETTINGS This page is used to set up an experiment Enter the name of the first reactor line under Channel 1 A channel name is limited to 10 characters In the Use attribute select In use Save the above settings by pressing the Apply button Repeat this procedure for all the channels that will be used in the experiment and press Apply after each channel has been set The value of the current flow rate for each channel is also displayed The value is a mean value over the last minute 36 bloprccess ONTROL GRAPH bloprocess ONTROL SS es erry aw 2044 2043 2044 2047 2048 2049 DH 25 DN 209 DM 2050 2090 2057 29030 WP WO 216 2M 20908 2104 mo 21 6 01 206 21 26 21 26 01 260 1 26 01 260 071 26 Q1 26 01 26 01 20 91 26 01 25 01 26 01 20 01 260 61 26 01 20 O1 28 01 26 01 20 01 2 Tov Copyright 2906 2015 Bioprocess Control Sweden ABR AB nghts reserved This section shows the Graphs page Start logging To start data logging the user has to press the green button labeled Start logging The button will turn grey and it will change its name to Stop logging Under the Start
21. evel in the gas flow measuring device is within the recommended range b Fill up with additional deionised or distilled water to the recommended water level when necessary c Note periodically the parameters of interest e g volume flow rate time duration of the experiment by reading the values from the LCD screen of the system Important If the instantaneous flow rate is out of range i e gt 4000 Nml h the value displayed by the system will be marked with an asterisk 1 e 4000 The same symbol will be displayed in front of the flow rate value if the corresponding value is not a measured value but an estimated one This situation can appear for example when the tubing from the gas source and the unit is disconnected and the last value for the flow rate is displayed after a corresponding interpolation 18 bloprcocess ONTROL 8 4 End of Operation a Note the total running time if this is of interest In the displayed values the last data collected will be from the last opening of a cell b If an automatic data acquisition system is connected to the unit then generate a report Download the report and open it to make sure that the report has been generated properly and that no errors occurred while downloading the file c Stop the logging by pressing the Stop button in the Graph page Disconnect the power adapter from the wall socket Note When pressing the stop button the experiment can no longer be continued d D
22. f liquid displacement and buoyancy a digital pulse is generated when a defined volume of gas flows through the device The unit offers a standard signal output for automatic data acquisition and provides users with an embedded timer and reset button for easy logging of accumulated gas flow volume over a defined period of time The uFlow automatically normalises gas flow and volume measurements with real time temperature and pressure compensation This allows for gas measurements and data presentation to be standardised and thus the impact of measurement conditions are minimised The temperature and pressure of the gas are measured every time a flow cell opens allowing the user to derive exact kinetic information compensated for any variation over time The volumes are normalised to 0 C and 1 atm There are two versions of the uFlow Gas Meter available One has the power supply and analogue output connectors on the front 7 1 and the other has a combined port for power supply and analogue output on the side 7 2 bloprccess ONTROL 7 1 uFlow Gas Meter with Front Power and Analogue Output Port bioprocess ONTROL e uFlow e e e 5 volume Nml time min flow rate Nml h X version 2 2 D 9 10 11a 11b 1 Outlet for gas flow 7 Glass window for visual inspection of the flow cell movement 2 Level gauge 8 Liquid Crystal Display LCD for real time visualisation of normalised gas volume amp
23. front of the Data Loggers The Data Logger with a USB interface is shown to the left and the Universal Data Logger with Ethernet interface to the right 16 c d e f bloprccess ONTROL Insert the other end of the analogue signal cable to a uFlow Gas Meter Important If the Universal Data Logger is used please refer to the Universal Data Logger Operation and Maintenance Manual before connecting the signal cables The Universal Data Logger has more options for measuring parameters and signal processing types If more reactors will be used in the experiment repeat step b and c for each reactor and corresponding measuring device When all reactors and gas meters have been connected plug the power adapter into the power input at the back of the Data Logger Then connect it to a wall socket Please note The characteristics for the power adapters for the Data Logger and the uFlow Gas Meter are different so make sure to use the correct one See Chapter 7 for technical characteristics Enter the names and settings of the reactors in the Data Logger software See Software user guide chapter If the Universal Data Logger is used please refer to the Software chapter in Universal Data Logger Operation and Maintenance Manual GAS PIPE CONNECTION a b c Connect the gas generation source e g bioreactor to the gas flow inlet 4 preferably with a tube from material which is air tight e g Tygon The g
24. h can be reloaded by pressing the Load Data button in the Load Data window It may take a little longer for the data to show up in the Graph window than it does in the Load Data window AutoScale X Automatically scales the x axis to contain all data AutoScale Y Automatically scales the y axis to contain all data Export A picture of the current view of the graph can be exported as a picture excel file or added to the clipboard To export the experiment data see the Load Data page guide Clicking on the icon associated with a specific graph selected commands Left or right clicking the icon associated with a specific graph can reach additional software features Selected functions will be described below AN Plot Visible Common Plots Color d Line Style b Line Width Ld Anti Aliased Bar Plots d Fil Base Line p Interpolation p Point Style b X Scale p Y Scale p Export b Plot Visible This option toggles the visibility of the selected plot and is equivalent to the user clicking on the checkbox located to the left of the specific line s name 39 bloprccess ROL Colour This setting allows the user to define a different colour for the particular line Export A picture of the current view of the graph can be exported as a picture excel file or added to the clipboard To export the experiment data see the Load Data page guide LOAD DATA Start time va time s 01 00 m Afters m E
25. heet o D bioprz ce o yFiow RU ie e A a LJ p sib Copyright 2006 2015 Bioprocess Control Sweden AB All rights reserved The software interface 1s organised in a number of pages all reachable from the start page named Home The pages are laid out in the same order that an experiment is set up executed monitored and finally documented The overview below describes the pages available and their main functions Home This page is shown when the software is started The different pages can be accessed through the five tabs at the top Under the tabs links will lead the user to information such as the user manual the technical specification and product information sheets Settings In the Settings page the user prepares an experiment by applying individual settings for each reactor line represented by channels in the Data Logger system Graph The Graph page is where an experiment is started and stopped The graph provides a visual representation of the gas flow rates vs time It is possible to scroll and zoom in the graph and also to export pictures of it 34 bloprccess ROL Load Data The Load Data page allows the user to load data from a specific time interval and to generate a report of it The report contains experiment data for each reactor line System This page contains general information related to the software version Data Logger hardware and contact d
26. ime bloprccess ON TT Raa www bioprocesscontrol com 14 bloprcocess ONTROL 8 OPERATION In this section references are given to pictures in the Chapter 7 Equipment Description in the form of a number within parenthesis e g 1 8 1 Before Start up INSTALLATION OF THE uFLOW GAS METER a b c d e Install the instrument on a flat and stable surface Use appropriate instrumentation 1 e bubble level device to verify the horizontality of the surface and to adjust its position if it is needed Use the adjustable foot 9 and the level gauge 2 to ensure the correct installation position Fill up the measurement chamber with the packing liquid e g deionised or distilled water through the port specially designed for filling the packing liquid 3 by using a small syringe It is also possible to fill the instrument through the gas flow inlet 4 with the help of a small syringe Check the water level in the chamber It should be within the limits of the marking A water level below the minimum acceptable level 6 can cause errors in the measurement of the volume and flow rate of the gas If the uFlow Gas Meter will be used as a stand alone unit 1 e without a Data Logger system connect the power cable to the power connector on the front 11a or to the combined power and analogue output port on the side 11 depending on what version of the uFlow Gas Meter is used Then connect the power adapter
27. isconnect the tube connection between the gas source e g reactors and the uFlow e Empty the water from the measurement chamber by opening the specific port 3 or the eas flow inlet port 4 9 MAINTENANCE When the carrier gas contains small particles these particles can lead to sedimentation in the measuring chamber When the experiment 1s stopped and the electrical connection and all the tubing are disconnected the chamber can be cleaned by emptying and refilling the chamber using the proper port when the meter 1s kept upside down The procedure is repeated until no sediments are present at the bottom of the chamber If the uFlow is not in use for a longer period it is recommended to empty the measurement chamber and to store it 1n dry conditions 19 bioproc TT 10 FLOW SOFTWARE 10 1 Installation of the Flow Data Logger Software Windows 8 The procedure below describes the installation of the uFlow Data Logger Software The installation is divided into two steps The files needed for the first part is collected in a folder called MyFlowDAQ Additional Installers which also includes required installations of Adobe Reader and Open Office The second part is collected in the uFlow Installers Installation instructions for the Universal Data Logger can be found in the Universal Data Logger Operation and Maintenance Manual 1 Insert the USB card with the uFlow Data Logger Software Open the disk drive in Windows Exp
28. lorer and open the SW Installation folder Next open the folder called uFlow Data Logger and then the MyFlowDAQ Additional Installers folder 4 by Ope FH Select all 85 Select none Delete Rename Properties ny M n folder rs History p Invert selection New Open T L ThisPC USBDISK F SW installation uFlow Data Logger 5 Search uFlow JO P E Videos Name Date modified Type Size i Windows C cx RECOVERY D e USB DISK F fseventsd Spotlight V100 Trashes 4s SW_IF_screenst 4 SW installation 4 MyFlowDAQ Additional Installers 02 02 p uFlow Installers 13 02 22 16 File folder 10 24 File folder 015 015 ta Kl atas rl 2 items 20 2 Open the Volume folder af Home Share Hg X cut Copy Paste Clipboard Copy path fa Paste shortcut View le d X e Copy Delete Rename Move toy toy T Organise g 7 d uFlow Data Logger MyFlowDAQ Additional Installers ia Windows C cx RECOVERY D ex USB DISK F i fseventsd Spotlight V100 Temporarylter Trashes J SW installation a SW_IF_screenst i SW installation Gia Network 4 items 1 item selected 3 Select the setup exe P File Home Share Copy A cut MJ Copy path Paste Clipboard a Paste shortcut Name Ji AdobeReader Config J Docs Volume View Manage Date modified 02 02 2015 21 16 02 02 2015
29. ly more expensive the Universal Data Logger unit with Ethernet interface offers more functionality by allowing the user to set each channel to measure not only gas flow but also temperature pH and methane content It also has a greater flexibility with regards to distance cable length up to 100 m between Data Logger and computer as well as a more stable connection For more information about the Universal Data Logger please read the Universal Data Logger Operation and Maintenance Manual In short if the aim of the uFlow user is to view real time data in the immediate vicinity of the device under test and store the data on the local hard driver a Data Logger unit with USB interface 1s the economical choice If on the other hand the user 1s interested in a product with more functionality flexibility and reliability of data communication over a long period of time the Universal Data Logger unit with an Ethernet interface 1s the best choice The Data Logger software automatically logs the gas flow rate shows a real time visualisation of the flow rate trend curve as well as provides report generation One Data Logger system can support up to 8 uFlow devices A Data Logger unit is not included in the standard uFlow package A screenshot of the software user interface is presented below oloprccess ON TROL new 1041 can N A NIA NAS N AS N A a
30. o folder Clipboard Organise New Open Select BF Invert selection gt Jj gt ThisPC USBDISK F gt SW installation uFlow Data Logger v Search uFlow D B Videos Name Date modified Type Size i Windows C cx RECOVERY D ex USB DISK F a fseventsd Spotlight V10C p Trashes Ji SW IF screenst J SW installation Jk MyFlowDAQ Additional Installers 02 02 201522 16 File folder J uF low Installers 13 02 2015 10 24 File folder eas Rletharnrl 2 items 26 11 Open the Volume folder Home Share View 09 Y cut E J ig Open EB select an Copy path A Edit 38 Select none Paste Move Copy Delete Rename Properties z fa Paste shortcut toy to Ps History oo Invert selection Clipboard Organise Open Select gt n SW installation d uFlow Data Logger b uFlow Installers v A Search uFlow lL P BE Videos Name Date modified Type Size iip Windows C cx RECOVERY D ex USB DISK F ds fseventsd Spotlight V10C Trashes di SW_IF_screenst J SW installation J Volume 13 02 2015 10 24 File folder eu Metas rl 1 item 12 Select the setup exe file and open it File Home Share View 09 X cut we X xfi g Open gt fH Select att M Copy path T Edit 88 Select none Copy Paste _ Move Copy Delete Rename New Properties fa Paste shortcut toe tor folder s History Clipboard Organise New Open Select BF Invert selection
31. oreover in order to compare the data obtained from different laboratories correction factors need to be included in volume calculations to obtain gas volumes at standard temperature and pressure thus reducing or eliminating the error from this source 2 2 Advantages of uFlow over Conventional Flow Meters The uFlow provides several unique functionalities that are important for monitoring the gas flow rate during a biodegradation process e Large linear detection range 20 to 4000 ml h that fits perfectly with most lab and small pilot scale applications e Real time pressure and temperature compensation for gas flow rate and volume measurements ensure that all gas flow measurements are accurate e Real time gas flow rate and normalisation allow for gas measurements to be standardised bloprccess ROL Standard analogue current signal output for automatic data acquisition 4 20 mA Low maintenance requirements LCD display for visualisation of variables The design and calibration delivers highly precise measurements One of the most cost competitive solutions on the market today bloprccess ONTROL 3 DELIVERY CHECKS At delivery unpack and check that the contents match with what is listed below in the Section Box Content If the packaging or the equipment is broken or damaged at delivery please a Document and take photos of the parts and packaging b Inform the transport company at the time of delivery c Make sure that th
32. performance of the product BPC reserves the right to correct any possible text and image errors as well as changes to technical data in this manual Before Getting Started Read this manual before installing and using the equipment Keep the instruction manual for future reference and make sure it is easily available for people who regularly use the uFlow If the Universal Data Logger is used with the uFlow Gas Meter please read the Universal Data Logger Operation and Maintenance Manual 7 bloprccess ONTROL 6 SAFETY CONSIDERATIONS The uFlow should be used in an environment with good ventilation preferably in a laboratory fume hood especially when this detection unit is connected to bioreactors which are generating very high volumes of toxic or slightly aggressive gases Always be cautious when handling electrical devices close to water bloprccess ONTROL 7 EQUIPMENT DESCRIPTION DESIGN FUNCTION The uFlow is a flow meter for ultra low gas flow detection Its design and highly precise calibration delivers a measurement precision of 1 with a resolution of about 10 ml Therefore the uFlow meets the needs of the most demanding biogas biofuel labs and user applications The unit contains a glass window allowing for visual inspection of the flow cell movement and has an adjustable foot to ensure the correct installation position for high precision measurements The measuring device works according to the principle o
33. u Flow Gas flow meter Operation and Maintenance Manual Goon he fy ie e e amp o bioprzce3 g Flow u Flow Release History Version 2 0 September 2015 Any questions related to this Operation and Maintenance manual should be directed to Bioprocess Control Sweden AB Scheelev gen 22 SE 223 63 Lund Sweden Tel 46 0 46 163950 Fax 46 0 46 163959 E mail info bioprocesscontrol com or support bioprocesscontrol com Web www bioprocesscontrol com This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of Bioprocess Control Sweden AB Copyright 2009 2015 Bioprocess Control Sweden AB Produced in Sweden TABLE OF CONTENTS I PREFACE 9 95 59 Nt oe E ree En En CU bad Menem rere mane re ere 1 Z GENERAL TNFORMATION ee E E E 2 Zi ConvemtiOnial Gas RIVE TOUT D SOLUS 2 2 2 Advantages of uFlow over Conventional Flow Meters esses 2 3 DELIVERY CHECKS vetesscocdsssnecctecesccucdececenctebaceud Eee tube EEE EE E E 4 oL blow GaS Meter Bor C ono odes tects dene t ertet aed dt oi D mesi doses 4 o2 ObpuoBDdl PitSrle fitus ded oec tides ub bot ehtrtasd ioa oec eid dV eot otis a ene TURN OE ere 4 4 PRE CONIMISSIONENU ttes Ete eta i Eee ea opas CEDE eatis Re Es
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