Home

Agilent 1290 Infinity II High Speed Pump User Manual

1. Pump head parts in contact with the piston seal need to be cleaned properly in order to get a smooth surface and a tight connection 111 7 Maintenance 7 To remove wear use isopropanol 9 To dry the pump head and to remove residues use 8 To clean the piston and the pump head surface use the pressurized air 1 2 and 3 ultra fine abrasive mesh p n 8660 0852 10 If the wash seal shall be replaced by a new one use the steel side of the insert tool for removing it Wash seal 112 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance Replacing the Heat Exchanger Tools required p n 5023 2501 Parts required p n G4220 81013 G4220 81012 64220 20028 G4220 20001 Description Wrench 19 mm Screwdriver Torx T10 Description Heat Exchanger secondary pump head only Heat Exchanger secondary pump head only Headless screw for 1290 Infinity pump heads Spacer Fitting Preparations Remove the pump head assembly from the pump Remove the secondary pump head from the link plate CAUTION Loss of small spacer fitting Inside the secondary pump head is a small spacer fitting which can be dropped easily when removing the heat exchanger gt The heat exchanger does not need to be removed for pump head maintenance 7 Agilent 1290 Infinity lI High Speed Pump User Manual 113 7 Maintenance Pump Head Procedures 1 Remove the 19 mm screw at th
2. _ j Joes TE 8 Perform a Pump Leak Rate Test and a System Pressure Test Agilent 1290 Infinity Il High Speed Pump User Manual 129 7 Maintenance Replace the Purge Valve Head When Parts required Preparations CAUTION In case of problems with the purge valve p n Description thd Purge valve head 5067 4655 Capillary ST 0 25 mm x 235 mm G4220 87000 Capillary ST 0 17 mm x 300 mm 01090 87308 Capillary ST 0 25 mm x 130 mm Remove all capillary connections to the purge valve Potential damage of valve head or malfunction of valve When the pump is switched on the valve tag is accessed read write and used for correctly positioning the valve If the valve head is replaced while the pump is on invalid information may be written to the valve head making it unusable or positioning may be wrong resulting in wrong flow connections inside the valve potentially damaging parts gt Switch off the pump before working on the purge valve CAUTION Bias measurement results The valve drive contains sensitive optical parts Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results gt Protect the optical parts from dust and other pollutions 130 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 1 Remove all capillary connections Then unscrew the black union nut and remove the head of the purge valve by pulling it to
3. If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 10 Forced Cold Start Settings On board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 0 0 0 1 178 Agilent 1290 Infinity Il High Speed Pump User Manual Hardware Information 10 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined b
4. Please contact your Agilent service representative Please contact your Agilent service representative 53 54 Error Information Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective power switch assembly Please contact your Agilent service representative 2 Loose connection between the power switch Please contact your Agilent service representative board and the main board Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main boar
5. Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals Procedure is valid for primary and secondary pump head You may assemble pump head parts only correctly If parts are in wrong orientation the screws do not fit in Seals Always orientate the seals with the spring side towards the part Store seals in isopropanol and fit seals in wet Agilent 1290 Infinity Il High Speed Pump User Manual 99 7 Maintenance Pump Head Procedures HINT Always ultrasonicate seals in solvent to clean seals properly Obey the following order to clean seals properly 1 H30 tab 2 H30 dest 3 H20 lsopropanol 50 50 4 isopropanol 100 NOTE If the pump head has been initialized without pump this may have damaged the pump drive In this case please contact your local Agilent service organisation 100 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 Pump Head Procedures Remove the Pump Head Assembly Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 5043 1361 Hex Key Set Driver 5023 2499 Hex Key Set NOTE This procedure describes the replacement of the left pump head assembly Similarly the right pump head assembly can be replaced One pump head assembly consists of two pump heads which are both removed at the same time 1 In Lab Advisor go to Tools gt Remove Install Pump Head 2 Open the doors and follow instruction
6. LAN The inter module communication is done via CAN Agilent 1290 Infinity Il High Speed Pump User Manual 171 10 Hardware Information e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e USB Universal Series Bus as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler 172 Agilent 1290 Infinity Il High Speed Pump Use
7. Parts required Preparations CAUTION If the module shall be transported or shipped p n Description 9301 0411 Syringe Plastic 9301 1337 Syringe adapter G7120 44000 Protective Foam Flush both solvent channels with isopropanol Mechanical damage gt For shipping the module insert the Protective Foam to protected the module from mechanical damage Be careful not to damage tubing or capillary connections while inserting the module in the Protective Foam 136 Flush system with appropriate storage solution for example 20 isopropanol in water Remove solvent inlets from solvent reservoirs Disconnect the solvent tubing from the inlet of primary pump heads for both solvent channels Use a syringe for removing liquid from the solvent tubings between solvent reservoir shutoff valve panel solvent selection valve degassing unit and pump inlets Switch the solvent selection valve if applicable Remove tubing and capillary connections to other modules and the solvent cabinet Remove tubing plugs 4 Remove the shutoff valve panel by pulling it downwards 5 You may keep internal tubing and capillary connections Remove cable connections to other modules Remove the module from the stack Carefully insert the Protective Foam to the front part of the instrument Do not damage any tubing or capillary connections Close the front cover For transport or shipment put the module and accessory kit to the original s
8. The new solvent will now appear in the Solvent Type Catalogs Agilent 1290 Infinity II High Speed Pump User Manual Agilent 1290 Infinity II High Speed Pump User Manual KP amp e e e 20 ee 5 7 e Troubleshooting and Diagnostics 7 i User Interfaces 44 Agilent Lab Advisor Software 45 Overview about the troubleshooting and diagnostic features te Agilent Technologies 43 5 Troubleshooting and Diagnostics User Interfaces Depending on the user interface the available tests and the screens reports may vary e Preferred tool should be Agilent Lab Advisor Software see Agilent Lab Advisor Software on page 45 The Agilent OpenLab ChemStation C 01 03 and above do not include any maintenance test functions e Screenshots used within these procedures are based on the Agilent Lab Advisor Software 44 Agilent 1290 Infinity Il High Speed Pump User Manual Troubleshooting and Diagnostics 5 Agilent Lab Advisor Software The Agilent Lab Advisor Software is a standalone product that can be used with or without chromatographic data system Agilent Lab Advisor helps to manage the lab for high quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status Early Maintenance Feedback counters EMF instrument configuration information and diagnostic tests By the push of a button a detailed diagnostic report can be generated Upon re
9. gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed WARNING Incorrect line voltage at the module Shock hazard or damage of your instrument can result if the devices are connected to line voltage higher than specified gt Connect your module to the specified line voltage WARNING Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 18 Agilent 1290 Infinity Il High Speed Pump User Manual Site Requirements and Specifications 2 Power Cords Country specific power cords are available for the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Agilent makes sure that your instrument is shipped with the power cord that is suitable for your particular country or region WARNING Absence of ground connection The absence of ground connection can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection W
10. 1290 Infinity II High Speed Pump G7120A introduction and specifications e using and optimizing e troubleshooting and diagnose maintenance e parts identification e hardware information safety and related information Agilent Technologies 2014 2015 Printed in Germany 07 2015 G7120 90000 Rev D fe Agilent Technologies
11. 184 When the connection was established successfully the module responds with the following ET C AWINDOWS system32 cmd exe telnet 134 40 27 95 Agilent Technologies G1i315C PP 8000624 Figure 37 A connection to the module is made Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 3 Type and press enter to see the available commands WINDOWS system32 cmd_exe telnet 134 40 27 95 Agilent Technologies G1315C PP 8880824 gt command syntax description display help info display current LAN settings ip lt x x x x gt set IP Address sm X X X X gt set Subnet Mask gu lt x x x x gt set Default Gateway exit exit shell gt Figure 38 Telnet Commands Table 17 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip lt X X X xX gt sets new ip address sm lt X X X X gt sets new subnet mask gw lt X X X x gt sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style parameter value for example ip 134 40 27 230 Then press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return Agilent 1290 Infinity Il High Speed Pump User Manual 205 11 LAN Configuration 5 Use the and press Enter to li
12. 3 Close the Services and Administrative Tools screens 202 Agilent 1290 Infinity II High Speed Pump User Manual LAN Configuration 11 Manual Configuration Manual configuration only alters the set of parameters stored in the non volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Non Volatile RAM Stored Parameter Ld Control Module Figure 35 Manual Configuration Principle Agilent 1290 Infinity II High Speed Pump User Manual 203 11 LAN Configuration With Telnet Whenever a TCP IP connection to the module is possible TCP IP parameters set by any method the parameters may be altered by opening a Telnet session 1 2 204 Open the system DOS prompt window by clicking on Windows START button and select Run Type cmd and press OK Type the following at the system DOS prompt c gt telnet lt IP address gt or c gt telnet lt host name gt E C AWINDOWS system32 cmd _exe C gt telnet 134 46 27 95 Figure 36 Telnet Starting a session where lt IP address gt may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switch on page
13. BootPTAB 0010835675AC Status BootP Request finished processing at outermost layer 3 Record the hardware MAC address for example 0010835675AC Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 4 The Error means the MAC address has not been assigned an IP address and the Tab File does not have this entry The MAC address is saved to the Tab File when an IP address is assigned 5 Close the log file before turning on another instrument 6 Uncheck the Do you want to log BootP requests box after configuring instruments to avoid having the logfile use up excessive disk space Adding each instrument to the network using BootP 1 Follow Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 Uncheck the Do you want to log BootP requests once all instruments have been added The Do you want to log BootP requests box must be unchecked when you have finished configuring instruments otherwise the log file will quickly fill up disk space 3 Click Edit BootP Addresses The Edit BootP Addresses screen appears 4 Click Add The Add BootP Entry screen appears Add BootP Entry x Figure 32 Enable BootP logging Agilent 1290 Infinity II High Speed Pump User Manual 199 11 LAN Configuration 5 Make these entries for the instrument e MAC address Host name Enter a Hostname of your choice The Host Name must begin with alpha charac
14. Example The user may not want to have a Bootp Server be active in his network all the time But on the other side he may not have any other configuration method than Bootp In this case he starts the Bootp Server temporarily powers on the module using the initialization mode Bootp amp Store waits for the Bootp cycle to be completed closes the Bootp Server and powers off the module Then he selects the initialization mode Using Stored and powers on the module again From now on he is able to establish the TCP IP connection to the module with the parameters obtained in that single Bootp cycle Bootp Active Server Parameter Non Volatile RAM Stored Parameter Figure 25 Bootp amp Store Principle Use the initialization mode Bootp amp Store carefully because writing to the non volatile memory takes time Therefore when the module shall obtain its parameters from a Bootp Server every time it is powered on the recommended initialization mode is Bootp Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 Using Stored When initialization mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Non Volatile RAM Active Parameter Stored Parameter Figure 26 Using Stored Principle Using D
15. ID 22405 Writing the pump encoder tag has failed e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Defect connection between encoder and Please contact your Agilent service main board representative 2 Defect tag Defect connection between tag Please contact your Agilent service and encoder representative Pump drive blocked or encoder failed Error ID 22406 Pump drive blocked or encoder failed e Parameter None Probable cause Suggested actions 1 Blockage of the pump drive Drive encoder Please contact your Agilent service failed representative 62 Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 Drive current too low Error ID 22407 The current consumption of the pump drive is too low Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Drive motor defect Please contact your Agilent service representative 2 Wrong missing connection of pump drive to Please contact your Agilent service main board representative Drive Encoder failed Error ID 22408 Drive encoder failed during pump drive calibration Probable cause Suggested actions 1 Internal error Contact Agilent support Agilent 1290 Infinity Il High Speed Pump User Manual 63 64 Error Information Drive current too high Error ID 22409 The current consumption of the pump drive is too high e Parameter 1 4 referring to pump drive Probable cau
16. Information Defect connection between main board and pump drive encoder Error ID 22414 Defect connection between main board and pump drive encoder e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Defect connection between main board and Please contact your Agilent service pump drive encoder representative 2 Defect encoder Please contact your Agilent service representative Pump drive encoder defect Error ID 22415 Defect pump drive encoder e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Defect encoder Please contact your Agilent service representative 66 Agilent 1290 Infinity II High Speed Pump User Manual Error Information Purge valve failed Error ID 22417 Lost steps of the purge valve encoder e Parameter None Probable cause Suggested actions 1 Purge valve drive mechanically blocked or e Check installation of purge valve head defect e Please contact your Agilent service representative Reading of purge valve tag failed Error ID 22420 Reading the purge valve tag failed e Parameter None Probable cause Suggested actions 1 Reading of purge valve tag failed Check cable connection 2 Purge valve head tag defect or empty Replace purge valve head 3 Purge valve tag reader is defect Please contact your Agilent service representative Agilent 1290 Infinity Il High Speed Pump User Manual 67 6 6 Error Information Pump drive encod
17. Pump G7120A Performance Specifications Feature Specification Integrated degassing Included unit Automatic Purge Valve Active Seal wash Intelligent System Emulation Technology ISET Communications Safety and maintenance GLP feature Housing Number of channels 2 Internal volume per channel 1 5 mL Materials in contact with solvent TFE PDD Copolymer FEP PEEK PPS Included Included Included Controller area network CAN RS232C APG remote ready start stop and shutdown signals LAN Extensive diagnostics error detection and display through included Agilent LabAdvisor leak detection safe leak handling leak output signal for shutdown of the pumping system Low voltage in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre defined and user settable limits and feedback messages Electronic records of maintenance and errors All materials are recyclable Agilent 1290 Infinity Il High Speed Pump User Manual 23 2 Site Requirements and Specifications Performance Specifications 24 Agilent 1290 Infinity II High Speed Pump User Manual Agilent 1290 Infinity II High Speed Pump User Manual 3 Using the Pump Magnets 26 Turn on off 27 Status Indicators 28 Best Practices 29 Daily Weekly tasks 29 Power up Shut down the pump 29 Prepare the pump 30 How to deal with solvents 31 Nor
18. Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces The module includes a DC Out 24 VDC power line that is intended to be used with certain modules that operate as CAN slaves for example external valves The line has a limited output of 0 5 A 1 7 A as of August 2011 and is self resetting Agilent 1290 Infinity lI High Speed Pump User Manual 175 10 Hardware Information Setting the 8 bit Configuration Switch The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communicat
19. Secondary pump head parts 144 Agilent 1290 Infinity II High Speed Pump User Manual Parts and Materials for Maintenance 8 Secondary Head G4204 60061 Item 1 2 3 10 11 12 13 14 p n 0515 1218 G4220 20003 G4220 20000 G4220 81013 G4220 81012 G2404 25213 G4220 20028 G4220 20001 0905 1719 5023 2513 G4220 60016 01018 07102 0905 1718 G4220 63010 64220 23705 G4220 60000 5067 5938 Description Screw M5 40 mm long Pump Head Screw LID Heat Exchanger Heat Exchanger secondary pump head only not shown PH Body sec Headless screw for 1290 Infinity pump heads not shown Spacer Fitting not shown PE Seal Distance sheet Seal holder including backup ring Gasket Seal wash Backup Seal PE Seal Wash Support Ring Seal Wash Bracket Preload Assy Plunger Agilent 1290 Infinity II High Speed Pump User Manual 145 8 Parts and Materials for Maintenance Purge Valve Purge Valve 146 Figure 13 Item ee wo N p n tbd 1535 4857 5068 0004 5068 0201 1535 4045 Purge valve parts Description Purge valve head Stator screws Purge Valve Stator Purge Valve Rotor Seal polyimide 1300 bar Bearing ring Agilent 1290 Infinity II High Speed Pump User Manual Cover Parts p n 5043 0286 5067 5908 G4224 60200 G4224 60201 5067 5767 5067 5768 Leak Parts p n 5043 0856 Parts and Materials for Maintenance Description Base Cover Top Cover Side Cover R
20. USB Flash Disk Required tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Agilent 1290 Infinity II High Speed Pump User Manual 167 10 Hardware Information Main FW update Resident System Main System Resident FW Update Figure 17 Firmware Update Mechanism Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost A
21. and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use ESD protection for example an ESD wrist strap when handling electronic boards and components CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 80 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Overview of Maintenance The following pages describe maintenance simple repairs of the module that can be carried out without opening the main cover Agilent 1290 Infinity Il High Speed Pump User Manual 81 7 Maintenance Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 82 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Install Fittings and Capillaries WARNING Solvent can spray under high pressure gt Observe appropriate safety procedures for example goggles safety gloves and protective clothing when
22. can be configured by the following methods e by automatically requesting the parameters from a network based BOOTP Server using the so called Bootstrap Protocol by automatically requesting the parameters from a network based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 190 e by manually setting the parameters using Telnet e by manually setting the parameters using the Instant Pilot G4208A The LAN interface differentiates between several initialization modes The initialization mode short form init mode defines how to determine the active TCP IP parameters after power on The parameters may be derived from a Bootp cycle non volatile memory or initialized with known default values The initialization mode is selected by the configuration switch see Table 12 on page 185 Agilent 1290 Infinity II High Speed Pump User Manual 183 11 LAN Configuration Configuration Switch Configuration Switch The configuration switch can be accessed at the rear of the module Configuration switch Figure 23 Location of Configuration Switch The module is shipped with all switches set to OFF as shown above NOTE To perform any LAN configuration SW1 and SW2 must be set to OFF Table 11 Factory Default Settings Initialization Init Mode Bootp all switches down For details see Initiali
23. described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an opera
24. on page 201 or Restarting the Agilent BootP Service on page 202 5 Uncheck the Do you want to log BootP requests box after configuring instruments otherwise the log file will quickly fill up disk space Agilent 1290 Infinity lI High Speed Pump User Manual 197 11 LAN Configuration 198 Determining the MAC address directly from the LAN Interface card label 1 Turn off the instrument 2 Read the MAC address from the label and record it The MAC address is printed on a label on the rear of the module 3 Read the MAC address from the label and record it The MAC address is printed on a label on the rear of the module See Figure 21 on page 182 and Figure 22 on page 182 4 Turn on the instrument Assigning IP Addresses Using the Agilent BootP Service The Agilent BootP Service assigns the Hardware MAC address of the instrument to an IP address Determining the MAC address of the instrument using BootP Service 1 Power cycle the Instrument 2 After the instrument completes self test open the log file of the BootP Service using Notepad The default location for the logfile is C Documents and Settings All Users Application Data Agilent BootP LogFile The logfile will not be updated if it is open The contents will be similar to the following 02 25 10 15 30 49 PM Status BootP Request received at outermost layer Status BootP Request received from hardware address 0010835675AC Error Hardware address not found in
25. opening flow path CAUTION Deformation of fittings and seals Liquid drops under high pressure up to 1200 bar act like solid parts Tightening connections under high pressure can deform or destroy fittings and seals gt Never tighten flow connections under pressure The lifetime of a fitting depends on how firmly it has been tightened firm tightening reduces the lifetime If fitting has been overtightened replace it 1 Install fittings and capillaries 2 Tighten fittings and capillaries Agilent 1290 Infinity Il High Speed Pump User Manual 83 7 Maintenance Remove and Install Doors Parts required p n Description 5067 5767 Door assy 200 left IF II 5067 5768 Door assy 200 right IF II The figures shown in this procedure exemplarily show the Infinity II Multisampler module The principle of how to remove and or install doors works in the same way for all Infinity II modules 84 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Remove and Install Doors 1 Press the release buttons and pull the front door out 2 For the Installation of the front door Insert the hinges into their guides and move the door in until the release buttons click into their final position Agilent 1290 Infinity II High Speed Pump User Manual 85 7 Maintenance Replace the Shutoff Valve Panel When If a shutoff valve is damaged or the panel needs to be removed for other repair procedures Parts req
26. operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1290 Infinity lI High Speed Pump User Manual 219 12 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 220 Agilent 1290 Infinity Il High Speed Pump User Manual Appendix 12 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Agilent 1290 Infinity lI High Speed Pump User Manual 221 Index Index 8 bit configuration switch on board LAN 176 A Agilent Lab Advisor software 45 Agilent Lab Advisor 45 Agilent oninternet 221 User Interface Software Setup 208 ambient non operating temperature 21 ambient operating temperature 21 analog signal 1 74 apgremote 1 4 assemble primary pump head 118 secondary pump head 118 automatic configuration with Bootp 193 bench space 20 binary pump shutdown during analysis 61 BootP service installation 195 restart 202 settings 202 stop
27. prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as Agilent 1290 Infinity II High Speed Pump User Manual Hardware Information 10 e standard TTL levels 0 V is logic true 5 0 V is false e fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Table 7 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L
28. pump head which fixes the connection capillary of the heat exchanger 1 Then lift up the capillary and remove it from the primary pump head 2 A gold seal between outlet valve and heat exchanger capillary is used for a tight connection Agilent 1290 Infinity Il High Speed Pump User Manual 103 7 Maintenance Pump Head Procedures 3 Remove both pump heads from the link plate by pushing the clips at the rear of the plate Link plate 4 Remove the outlet valve at the top of the pump head and the inlet valve at the bottom of the pump head Outlet valve Inlet valve 104 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Disassemble the Primary Pump Head INET Damage of pump piston The pump piston is made of Zr07 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals 1 For disassembling the pump head remove the 4 hex 2 Remove the front part of the pump head including pump screws at the rear of the pump head chamber housing with pump seal distance sheet support ring seal holder including backup ring and the gasket Support ring fi l eal wash gasket AN Seal holder K s Distance sheet R i OY Pump chamber b housing a J I ey SO ST gw wa Agilent 1290
29. pump service kit 5067 4699 includes pump head alignment tool and items 1 5 Figure 14 Pump head alignment tool Agilent 1290 Infinity I High Speed Pump User Manual 149 8 Parts and Materials for Maintenance Tools Alignment piston Tool handle Figure 15 Replacement kit for 1290 Infinity pump head alignment tool piston handle Figure 16 HPLC System Tool Kit Infinity II 150 Agilent 1290 Infinity Il High Speed Pump User Manual HPLC System Tool Kit HPLC System Tool Kit Infinity II G7120 68708 contains the following items p n 8720 0025 8710 1924 8710 2409 8710 0510 5023 2500 8710 1534 5043 1361 5023 2499 8710 2394 8710 0899 5023 2501 5023 2504 5023 2503 5023 2502 9301 0411 9301 1337 0100 1710 0100 1681 01018 23702 5067 6127 Parts and Materials for Maintenance Description Wrench 1 2 inch amp 9 16 inch Wrench open 14 mm Wrench open end 5 16 3 8 inch Wrench open 1 4 5 16 inch Wrench Wrench 4 mm both ends open end Hex Key Set Driver Hex Key Set Hex key 9 64 inch 15 cm long T handle Pozidriv screwdriver Screwdriver Torx T10 Hex driver SW 4 slitted Hex driver SW 5 slitted Hex driver 1 4 inch slitted Syringe Plastic Syringe adapter Mounting Tool for Tubing Connections Syringe adapter luer barb Insert tool Blank Nut SL Agilent 1290 Infinity Il High Speed Pump User Manual 151 8 Parts and Materials for Maintenance HPLC System Tool Kit 152 Agi
30. specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt Ground the waste container gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly gt Do not use solvents with an auto ignition temperature below 200 C 392 F Agilent 1290 Infinity lI High Speed Pump User Manual 215 12 Appendix 216 Safety Symbols Table 18 Symbols m eber gt gt C The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up Cooling unit is designed as vapor compression refrigeration system Contains fluorinated greenhouse gas refrigerant according to the Kyoto protocol For specifications of refrigerant charge capacity carbon dioxide equivalent CDE and global warm
31. the front 2 Put the new valve head onto the valve drive such that the lobe fits to the groove Screw the valve head onto the valve drive using the union nut 3 Install all flow connections Port 1 is connected to the outlet of the secondary pump head of channel B Port 2 is connected to the outlet of the pressure sensor Port 3 is connected to the inlet of the pressure sensor Port 4 is connected the outlet of the secondary pump head of channel A Ports 5 and 6 are connected to waste capillaries The central port is connected to the Jet Weaver inlet Agilent 1290 Infinity II High Speed Pump User Manual 131 7 Maintenance Replace Parts of the High Pressure Filter Assembly When For removing blockages and leaks in the high pressure filter assembly The filter frit in the outlet valve should be replaced regularly depending on the system usage Other parts are covered by the Agilent Preventive Maintenance PM Service Tools required p n Description G4220 20012 Torque wrench 2 25 Nm G4220 20015 Adapter in square to hex 14 mm Hex bit Parts required p n Description 1 01018 22707 PTFE frits pack of 5 1 5067 4728 Seal cap CAUTION Leakage or damaged connection Opening the outlet of the primary pump head may cause leaks or damage the connection between the pump heads gt Do not open the outlet of the primary pump head This procedure describes replacements for channel A left pump head assembly and can be applied
32. to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 162 Agilent 1290 Infinity Il High Speed Pump User Manual Identifying Cables 9 Agilent Module to PC p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m Agilent 1290 Infinity lI High Speed Pump User Manual 163 9 Identifying Cables USB To connect a USB Flash Drive use a USB OTG cable with Mini B plug and A socket p n Description 5188 8050 USB A M USB Mini B 3 m PC Module 5188 8049 USB A F USB Mini B M OTG Module to Flash Drive Agilent 1290 Infinity Il High Speed Pump User Manual Agilent 1290 Infinity II High Speed Pump User Manual 10 Hardware Information Firmware Description 166 Electrical Connections 169 Serial Number Information 170 Rear View of the Module 170 Interfaces 171 Overview Interfaces 172 Setting the 8 bit Configuration Switch 176 Special Settings 178 Early Maintenance Feedback 179 Instrument Layout 180 This chapter describes the pump in more detail on hard
33. to the peristaltic pump inlet 1 and from the peristaltic pump outlet to the primary pump head inlet 2 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Release a Stuck Inlet Valve When If inlet valve is stuck or if pump is not generating pressure after being turned off for an extended period of time Before the system is turned off for an extended period of time it should be flushed with at least 10 isopropanol to prevent inlet valves from getting stuck 1 Remove the capillary connection from the outlet of the secondary pump head 2 Unscrew the tubing at the inlet valve 3 Attach a Luer lock syringe with adapter to the tubing and fill it with solvent 4 Reconnect tubing to inlet valve 5 Unscrew tubing at degassing unit and attach the syringe to it 6 Push solvent with syringe until it comes out at the top of the High Pressure Filter Assembly Detach the syringe and reconnect the tubing into the degassing unit 8 Reinstall the capillary connection to the High Pressure Filter Assembly 9 Purge the system to remove air Agilent 1290 Infinity Il High Speed Pump User Manual 97 7 Maintenance Pump Head Procedures CAUTION Limitation of life time The pump head assembly is an exchange part which cannot be reassembled with standard tools Disassembling the pump head will strongly limit its life time gt Do not disassemble the pump head assembly CAUTION Damage of connect
34. valve head 130 seal wash pump 96 solvent selection valve 94 wash seal 116 resolution Optimization 39 RS 232C cable 163 Agilent 1290 Infinity Il High Speed Pump User Manual S safety class 212 safety general information 212 standards 21 symbols 216 seal wash pump was missing when tried to turnon 73 secondary pump head assemble 118 disassemble 109 parts 144 serial number information 170 shutdown 50 shutoff valve panel replacing 86 shutoff valves replacing 86 site requirements 17 power cords 19 solvent counter exceeded limit 59 solvent selection valve replace 94 special interfaces 175 special settings boot resident 178 forced cold start 178 specification physical 21 specifications 17 SSV replace 94 T target pressure not reached for binary pump degasser 58 TCP IP parameter configuration 183 telnet Index configuration 204 temperature sensor 55 timeout 50 torques pumphead 89 transport prepare 136 troubleshooting errormessages 49 U unknown purge valve type 71 V valve rail kit install 134 voltage range 21 Ww wash seal replace 116 waste counter limit exceeded 60 waste electrical and electronic equipment 218 WEEE directive 218 weight 21 writing the pump encoder tag failed 62 writing the purge valve tag failed 69 Agilent 1290 Infinity II High Speed Pump User Manual 225 www agilent com In This Book This manual contains technical reference information about the Agilent
35. with the length of the column This is the reason that the 1290 Infinity LC system was designed to go to 1200 bar so that it can run sub two micron particles and column length can be increased to 100 mm or 150 mm There are even examples of 100 mm and 150 mm columns linked to give 250 mm length Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1 4 What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure Methanol mixtures will generate more back pressure than acetonitrile mixtures Acetonitrile is often preferred because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better selectivity certainly for small molecules less than about 500 Da The viscosity can be reduced by increasing the temperature but it should be remembered that this can change the selectivity of the separation Experiment will show if this leads to increase or decrease in selectivity As flow and pressure are increased it should be remembered that frictional heating inside the column will increase and that can lead to slightly increased dispersion and possibly a small selectivity change both of which could be seen as a reduction in resolution The latter case might be offset by reducing the temperature of the thermostat by a few degrees and again experiment will reveal the answer The van Deemter curve sho
36. 0 60070 1 G7120 60017 5067 5760 Description Capillary ST 0 25 mm x 235 mm purge valve to pump head assemblies channel A and B 2x Peristaltic pump with Pharmed tubing Tubing 1 mm i d 3 mm o d silicone 5 m Tubing kit 140 mm 2 pk SSV to shutoff valve or degassing unit Tubing kit 270 mm for connection of degassing unit to inlet valve set of 2 tubes Capillary ST 0 17 mm x 300 mm Valve to Jet Weaver Capillary ST 0 25 mm x 130 mm purge valve to pressure sensor 2x Valve Holder Left not shown Shutoff valve not shown Bottle Head Assembly not shown Ultra Clean Tubing Kit includes bottle head assemblies and tubing connections within the pump Tubing Kit 140 mm Ultra Clean Tubing tubes from SSV to shutoff valve or degassing unit to MCGV Bottle Head Assembly Ultra Clean Tubing bottle heads and tubing to shutoff panel degasser Solvent Cabinet Kit not shown Agilent 1290 Infinity Il High Speed Pump User Manual 139 8 Parts and Materials for Maintenance Pump Head Assemblies Figure 9 Pump head assembly left with seal wash option Figure 9 on page 140 exemplarily shows the pump head with seal wash option for channel A of the pump The following pump head assemblies are available p n Description G4220 60900 Pump Head Channel A with Seal Wash G4220 60910 Pump Head Channel B with Seal Wash All pump head assemblies are complete including valves and filter assemblies 140 Agilent 12
37. 0 Infinity II High Speed Pump G7120A Performance Specifications Feature Specification Hydraulic system Pump resolution step size Settable flow range Flow precision Flow accuracy Pressure range Pressure pulsation Compressibility compensation Recommended pH range Gradient formation Delay volume Composition precision Composition accuracy Number of solvent Solvent selection valve Two dual pistons in series pumps with proprietary servo controlled variable stroke design and smooth motion control 300 pL step size 0 001 5 mL min in 0 001 mL min increments executed in 300 pL step increments lt 0 07 RSD or 0 005 min SD whatever is greater 1 or 10 pL min whatever is greater up to 130 MPa 1300 bar at 0 2 mL min ramping down to 80 MPa 800 bar at 5 mL min lt 1 amplitude or lt 0 5 MPa 5 bar whatever is greater Automatic 1 0 12 5 solvents with pH lt 2 3 should not contain acid which attack stainless steel High pressure binary mixing As low as 45 uL 10 pL without mixer lt 0 15 RSD or 0 01 min SD whatever is greater 0 35 absolute 2 out of maximum 26 solvents Internal 4 solvent selection valve included External 2x 12 solvent valve as option fully integrated in the pump control interface Agilent 1290 Infinity II High Speed Pump User Manual Site Requirements and Specifications 2 Table 2 Agilent 1290 Infinity II High Speed
38. 0 Nm and close the outlet valve 2 7 Insert the heat exchanger capillary into the outlet of the outlet valve Using a torque wrench with a 2 5 mm hex bit set 3 Nm and close the hex screw at the top of the outlet 9 Open the shut off valves and purge the system to remove air Agilent 1290 Infinity Il High Speed Pump User Manual 93 7 Maintenance Replace the Solvent Selection Valve SSV When In case of problems with the solvent selection valve Parts required p n Description 1 G7120 60029 SSV Valve Assembly 2 G4220 60035 Tubing kit 140 mm 2 pk SSV to shutoff valve or degassing unit 1 Close shut off valve Remove tubing connections between the SSV and the solvent shut off valves and the SSV and the degassing unit inlets 2 Push down the SSV panel for removing it 3 Remove the connector by pushing up the small clip at the bottom of the connector 4 Install a new SSV by inserting the connector and clipping the SSV panel to the module top panel Then re install all tubing connections open shut off valve and purge valve 94 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Change Configuration or Replace the Jet Weaver When Tools required Parts required For optimizing the pump configuration to mixing performance or low delay volumes fast gradients see chapter Optimizing Performance p n Description 8710 0510 inch wrench 3 mm hex key p n Description 1 G42
39. 20 60027 Jet Weaver 35 pL 100 pL 1 G4220 60012 Jet Weaver 380 uL OPTIONAL 1 G4220 87000 Capillary ST 0 17 mm x 300 mm Valve to Jet Weaver 1 Remove capillary connections from the Jet Weaver 2 Remove the hex screws that fix the Jet Weaver to the pump housing The standard Jet Weaver Jet Weaver 35 uL 100 uL G4220 60027 has a front and a rear side with different internal volumes 35 100 uL that are optimized for a low delay volume or best mixing performance The optional Jet Weaver Jet Weaver 380 pL G4220 60012 is recommended for applications which are challenging with respect to mixing noise e g TFA applications and has just one side 3 Install new Jet Weaver or flip the Jet Weaver for backside 4 Reinstall the capillary connections The inlet at the left side of the Jet Weaver is connected to the central port of the pump valve by a capillary length 300 mm 0 17 mm i d The outlet at the right side is connected to the autosampler Agilent 1290 Infinity Il High Speed Pump User Manual 95 7 Maintenance Replace the Seal Wash Pump Replace the Seal Wash Pump When Parts required Preparations In case of wear of the seal wash pump p n Description 5065 4445 Peristaltic pump with Pharmed tubing 5065 9978 Tubing 1 mm i d 3 mm o d silicone 5m Remove the flow connections from and to the seal wash pump 1 Press the clips 2 Pull the pump to the front 4 Fix the seal wash tubings
40. 201 Bootp amp Store 186 automatic configuration 193 initialization modes 185 using default 187 using stored 187 222 c cable CAN 162 LAN 162 overview 154 RS 232 163 cables analog 156 remote 158 CAN cable 162 capillaries 83 cleaning 82 compensation sensor open 53 compensation sensor short 54 condensation 20 configuration switches 184 current of primary pump drive too high 70 current of secondary pump drive too high 70 D defect connection between main board and pump drive encoder 66 degasser s pressure limit violation 59 delay volume description 36 deliver underrun 65 DHCP general information 189 setup 190 dimensions 21 disassemble primary pump head 105 pump head assembly 103 secondary pump head 109 doors install 84 remove 84 drive current too high 64 drive current too low 63 drive encoder error 69 drive Encoder failed 63 drive position limit 68 drive timeout 64 E electrical connections descriptions of 169 electronic waste 218 electrostatic discharge ESD 80 EMF early maintenance feedback 179 error messages binary pump shutdown during analysis 61 compensation sensor open 53 compensation sensor short 54 cover violation 56 current of primary pump drive too high 70 current of secondary pump drive too high 70 defect connection between main board and pump drive encoder 66 degasser s pressure limit violation 59 deliver underrun 65 Agilent 1290 Infinity Il High Speed Pump User M
41. 5 LC Modules inclusive 1120 1220 configuration switch at rear of the instru ment SW6 SW7 SW 8 Initialization Mode ON OFF OFF DHCP 3 Turn on the module that hosts the LAN interface 4 Configure your Control Software e g Agilent ChemStation Lab Advisor Firmware Update Tool and use MAC address as host name e g 003043177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 189 Agilent 1290 Infinity lI High Speed Pump User Manual 191 11 LAN Configuration Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full or half duplex modes In most cases full duplex is supported when the connecting network device such as a network switch or hub supports IEEE 802 3u auto negotiation specifications When connecting to network devices that do not support auto negotiation the LAN interface will configure itself for 10 or 100 Mbps half duplex operation For example when connected to a non negotiating 10 Mbps hub the LAN interface will be automatically set to operate at 10 Mbps half duplex If the module is not able to connect to the network through auto negotiation you can manually set the link operating mode using link configuration switches on the module Table 16 Link Configuration Switches SW3 SW4 SW5 Link Configuration OFF speed and duplex mode determined by auto negotiation ON OFF OFF m
42. 52 Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the Power Switch board Please contact your Agilent service representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being pinched by a metal component Please contact your Agilent service representative Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the power switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Loose connection between the power switch board and the main board 2 Defective power switch assembly Agilent 1290 Infinity Il High Speed Pump User Manual
43. 90 Infinity Il High Speed Pump User Manual Pump Head Assembly Parts Figure 10 Item p n 1 G4220 81013 1 G4220 81012 2 G4220 60022 3 G4220 60028 4 G4220 20020 5 G4280 60026 Agilent 1290 Infinity I High Speed Pump User Manual Parts and Materials for Maintenance ibly Parts Pump Head Asse Pump head assembly parts Description Heat Exchanger secondary pump head only Channel A Heat Exchanger secondary pump head only Channel B Inlet valve primary pump head Outlet valve primary pump head Internal gold seal for Outlet Valve High Pressure Filter Assembly secondary pump head 141 8 Parts and Materials for Maintenance Primary Pump Head Parts Primary Pump Head Parts so Figure 11 Primary pump head parts 142 Agilent 1290 Infinity Il High Speed Pump User Manual Parts and Materials for Maintenance Primary Head G4204 60060 Item 1 2 wo 10 11 p n 0515 1218 G4204 60033 0905 1719 5023 2513 G4220 60016 01018 07102 0905 1718 G4220 63010 G4220 23705 G4220 60000 5067 5938 Description Screw M5 40 mm long PH Body prim PE Seal Distance sheet Seal holder including backup ring Gasket Seal wash Backup Seal PE Seal Wash Support Ring Seal Wash Bracket Preload Assy Plunger Agilent 1290 Infinity II High Speed Pump User Manual 143 8 Parts and Materials for Maintenance Secondary Pump Head Parts Secondary Pump Head Parts AD ofe D j Figure 12
44. ARNING Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use a power cord other than the one that Agilent shipped with this instrument gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Es fee Solvents may damage electrical cables gt Prevent electrical cables from getting in contact with solvents gt Exchange electrical cables after contact with solvents Agilent 1290 Infinity Il High Speed Pump User Manual 19 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1 on page 21 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Agilent recommends that you install the HPLC instrument in the A Line Flex Bench rack This option helps to save bench space as all modules can be placed into one single stack It al
45. Flow rate limit exceeded Error ID 22064 Suggested actions Empty waste container e Reset waste counter e Adjust waste counter limit The flow rate of the binary pump has exceeded the limit while the pump runs in pressure controlled mode e g during a pressure test Parameter None Probable cause 1 Leak 2 Bottle empty 3 Shutoff valve closed 4 Drift of pressure sensor unlikely for short tests taking some minutes 60 Suggested actions Check for leaks in the pump and flow path Fill solvent bottle Open shutoff valve Replace pressure sensor Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 Binary pump shutdown during analysis Error ID 22065 The binary pump has been shut down by the control software or control module during an analysis e Parameter 0 for off 1 for standby Probable cause Suggested actions 1 Pump has been shut down Restart pump Reading the pump encoder tag failed Error ID 22402 Reading the pump encoder tag has failed e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Defect connection between encoder and Please contact your Agilent service main board representative 2 Missing or defect tag Defect connection Please contact your Agilent service between tag and encoder representative Agilent 1290 Infinity Il High Speed Pump User Manual 61 6 Error Information Writing the pump encoder tag failed Error
46. I to general purpose p n 5188 8029 pin Color code Enhanced Classic Active TTL Remote Remote 1 white 101 START Low D Sub female 15way REQUEST user s view to connector 585355585 2 brown 102 STOP Low N fon uw w N p 8 1 3 green 103 READY High EEE EEEE 150 0 000 4 yellow 104 POWER ON High r a a aE 5 grey 105 NOT USED TERE era 6 pink 106 SHUTDOWN Low 3 7 blue 107 START Low 8 red 108 PREPARE Low 9 black 1wire DATA 10 violet DGND 11 grey pink 5V ERI out 12 red blue PGND 13 white green PGND 14 brown green 24V ERI out 15 white yellow 24V ERI out NC yellow brown 5188 8044 ERI to ERI Connector D_Subminiature 15 pin 158 Agilent 1290 Infinity Il High Speed Pump User Manual Table4 5188 8044 ERI to ERI Identifying Cables 9 p n 5188 8044 Pin ERI Signal Pin ERI Active TTL ne 9 10 GND 10 m EB 1 Start Request 1 Low 2 Stop 2 Low 3 Ready 3 High 5 Power on 5 High 4 Future 4 6 Shut Down 6 Low 7 Start 7 Low 8 Prepare 8 Low Ground Cable Shielding NC 5188 8045 ERI to APG Connector D_Subminiature 15 pin ERD Connector D_Subminiature 9 pin APG p n 5188 8045 Pin ERI Signal Pin APG Active TTL ae E 10 GND 1 are S 1 Start Request 9 Low 2 Stop 8 Low 3 Ready 7 High 5 Power on 6 High 4 Future 5 6 Shut Down 4 Low 7 Start 3 Low 8 Prepare 2 Low Ground Cable Shielding NC Agilent 1290 Infinity lI High Speed Pump User Manual 159 9 160 Identifying Cables
47. Infinity II High Speed Pump User Manual 105 7 Maintenance 3 Remove the piston from the piston housing by pushing it to the rear then pull it out from the rear 106 4 Check the pump pistons for scratches grooves and dents when changing the piston seals Damaged pistons cause micro leaks and will decrease the lifetime of the seals Piston surface Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 5 Use the steel side of the insert tool for removing the 6 To flush out particles from the pump head use pump seal from the pump chamber housing pressurized air 1 2 and 3 Pump seal Do not use the pump piston for that purpose as this can break it Pump head parts in contact with the piston seal need to be cleaned properly in order to get a smooth surface and a tight connection Agilent 1290 Infinity II High Speed Pump User Manual 107 7 Maintenance 7 To remove wear use isopropanol 9 To dry the pump head and to remove residues use 8 To clean the piston and the pump head surface use the pressurized air 1 2 and 3 ultra fine abrasive mesh p n 8660 0852 10 If the wash seal shall be replaced by a new one use the steel side of the insert tool for removing it Wash seal 108 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 Disassemble the Secondary Pump Head SVE yy Damage of pump pisto
48. Maximizing peak capacity and separation speed in liquid chromatography Agilent 1290 Infinity II High Speed Pump User Manual 41 4 Optimizing Performance Using Solvent Calibration Tables 42 Importing Solvent Calibration Tables RC NET based Agilent graphical user interfaces ChemStation EZChrom Elite OpenLab etc include data for most commonly used solvents in HPLC This data contains solvent properties and is used for optimum pump control in order to ensure best flow and composition accuracy If your solvent is not included to the software please check the Agilent web site http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 for additional libraries registration required which also provides updates and optimized data If your solvent is neither available in the user interface nor in the library please use generic solvents Generic aqueous gives good results for most solvent mixtures with at least 50 water which have similar properties as pure water For other solvents with high organic percentage Generic organic gives a good approximation Importing Solvent Calibration in ChemStation 1 Go to menu Instrument gt Instrument configuration 2 In the Instrument Configuration screen choose your module and click Configure Click Configure Solvent Type Catalogs In Solvent Type Catalogs click Import Navigate to the location of the solvent calibration table and click Open ao ol A Q
49. One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1290 Infinity II High Speed Pump User Manual Agilent Module to General Purpose Identifying Cables 9 p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo eee S S te Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low Agilent 1290 Infinity lI High Speed Pump User Manual 161 9 Identifying Cables CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module
50. Pump User Manual Maintenance 7 CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 9 Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 5 Nm and fix the 4 screws at the rear of the alignment tool Tighten screws crosswise KN This procedure will align pump head parts to their correct positions and close the pump head tightly 10 Open the 3 screws which have closed the pump head alignment tool and take out the aligned pump head In case the pump head sticks inside the alignment tool you can use the handle and insert it to the rear of the tool for pushing out the pump head Heat exchanger capillary ool han GO i Agilent 1290 Infinity II High Speed Pump User Manual 123 7 Maintenance CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 11 For the primary pump head install the inlet valve and outlet valve using the torque wrench which is included to the 1290 Infinity Service Kit p n 5067 4699 Set 10 Nm for the inlet valve and 10 Nm for the outlet valve 12 Replace the filter frit and seal cap as desired Please note the correct orienta
51. UTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions Agilent 1290 Infinity lI High Speed Pump User Manual 217 12 Appendix Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product R Do not dispose of in domestic household waste To return unwanted products contact your local Agilent office or see http www agilent com for more information 218 Agilent 1290 Infinity Il High Speed Pump User Manual Appendix 12 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is
52. Windows programs Insert the Agilent ChemStation software DVD into the drive If the setup program starts automatically click Cancel to stop it 4 Open Windows Explorer 5 Go to the BootP directory on the Agilent ChemStation DVD and double click BootPPackage msi If necessary click the Agilent BootP Service icon in the task bar The Welcome screen of the Agilent BootP Service Setup Wizard appears Click Next The End User License Agreement screen appears Read the terms indicate acceptance then click Next The Destination Folder selection screen appears Install BootP to the default folder or click Browse to choose another location Click Next The default location for installation is C Program Files Agilent BootPService 10 Click Install to begin installation Agilent 1290 Infinity lI High Speed Pump User Manual 195 11 LAN Configuration 11 Files load when finished the BootP Settings screen appears BootP Settings a BootP Tab File Create Tab File Edit BootP Addresses Logging BootP Log File ESS ad Sa REG Default Settings Subnet mask GO 0 0 0 Gateway GO 0 0 0 Cancel Help Figure 30 BootP Settings screen 12 In the Default Settings part of the screen if known you can enter the subnet mask and gateway Defaults can be used The default subnet mask is 255 255 255 0 The default gateway is 192 168 254 11 13 On the BootP Settings screen click OK T
53. accordingly to channel B In both cases maintenance is done only at the secondary pump head outlet which hosts the filter frit When replacing a PTFE frit consider replacing the seal cap as well in order to prevent leaks 132 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 Replace Parts of the High Pressure Filter Assembly 1 Remove the capillary connection from the outlet of the secondary pump head 2 Usea 14 mm hex wrench for opening the filter assembly of the secondary pump head 3 Replace the filter frit and seal cap as desired Please note the correct orientation of the filter frit Filter frit Seal cap 4 Re install the filter assembly using the torque wrench 14 mm hex bit set to 16 Nm 5 Reconnect the capillary from the purge valve to the high pressure filter assembly Agilent 1290 Infinity II High Speed Pump User Manual 133 7 Maintenance Install the Valve Rail Kit When Tools required Parts required 134 This rail is needed for the installation of external valves Description Pozidrive screwdriver 1 p n Description 1 5067 4634 Valve Rail Kit The rail can be installed on the left or right side of the pump This procedure describes the installation on the left side and applies similarly to the right side 1 The valve rail is fixed to the pump cover by 4 screws The position of the lower screws is marked on the modul
54. ace the Shutoff Valve Panel 86 Replace the Pressure Sensor 87 Overview of Torques for Pump Head Procedures 89 Replace the Inlet Valve 90 Replace the Outlet Valve 92 Replace the Solvent Selection Valve SSV 94 Change Configuration or Replace the Jet Weaver 95 Replace the Seal Wash Pump 96 Release a Stuck Inlet Valve 97 Pump Head Procedures 98 Remove the Pump Head Assembly 101 Disassemble the Pump Head Assembly 103 Disassemble the Primary Pump Head 105 Disassemble the Secondary Pump Head 109 Replacing the Heat Exchanger 113 Replace Wash Seal and Gasket 116 Assemble the Primary and Secondary Pump Head 118 Reassemble the Pump Head Assembly 127 Install the Pump Head Assembly 128 Brae Agilent Technologies 75 7 Maintenance Replace the Purge Valve Head 130 Replace Parts of the High Pressure Filter Assembly 132 Install the Valve Rail Kit 134 Replace the Module Firmware 135 Prepare the Pump Module for Transport 136 This chapter describes the maintenance of the High Speed Pump 76 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 Introduction to Maintenance Introduction to Maintenance Figure 4 on page 77 shows the main user accessible assemblies of the Agilent 1290 Infinity II High Speed Pump These parts can be accessed from the front simple repairs and don t require to remove the pump from the system stack Solvent Selection Valve Jet Weaver SSV Seal Wash Pump Seal Was
55. anual drive current too high 64 drive current too low 63 drive encoder error 69 drive Encoder failed 63 drive position limit 68 drive timeout 64 fan failed 54 flow rate limit exceeded 60 ignition without cover 55 55 insufficient power of drive encoder LED 68 leak sensor open 53 leak sensor short 52 leak 55 lost CAN partner 52 overcurrent of pump drive 65 overcurrent of solvent selection valve SSV 65 pressure below lower limit 58 pressure exceeded upper pressure limit 57 pressure sensor calibration wrong or missing 73 pump drive blocked or encoder failed 62 pump drive encoder defect 66 pump drive encoder error 71 pump drive encoder rollover 68 pump drive error 71 pump drive stop not found 72 pump drive stroke blocked 72 pump error 5 7 purge valve failed 67 reading of purge valve tag failed 67 reading the pump encoder tag failed 61 remote timeout 51 seal wash pump was missing when tried to turnon 73 shutdown 50 solvent counter exceeded limit 59 target pressure not reached for binary pump degasser 58 timeout 50 unknown purge valve type 71 waste counter limit exceeded 60 writing the pump encoder tag failed 62 writing the purge valve tag failed 69 extra column volume 36 F fan failed 54 firmware description 166 main system 166 resident system 166 update tool 167 updates 167 135 upgrade downgrade 135 fittings 83 flow connections 139 flow rate limit exceeded 60 frequency ran
56. anually set to 10 Mbps half duplex ON OFF ON manually set to 10 Mbps full duplex ON ON OFF manually set to 100 Mbps half duplex ON ON ON manually set to 100 Mbps full duplex 192 Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 Automatic configuration with Bootp All examples shown in this chapter will not work in your environment You need your own IP Subnet Mask and Gateway addresses Assure that the detector configuration switch is set properly The setting should be either BootP or BootP amp Store see Table 12 on page 185 Assure that the detector connected to the network is powered off If the Agilent BootP Service program is not already installed on your PC then install it from your Agilent ChemStation DVD located in folder BootP About Agilent BootP Service The Agilent BootP Service is used to assign the LAN Interface with an IP address The Agilent BootP Service is provided on the ChemStation DVD The Agilent BootP Service is installed on a server or PC on the LAN to provide central administration of IP addresses for Agilent instruments on a LAN The BootP service must be running TCP IP network protocol and cannot run a DHCP server Agilent 1290 Infinity Il High Speed Pump User Manual 193 11 LAN Configuration 194 How BootP Service Works When an instrument is powered on an LAN Interface in the instrument broadcasts a request for an IP address or host name and provides i
57. ap GL45 with 4 ports 5043 1221 for optimal and safe waste disposal If you decide to use your own waste solution make sure that the tubes don t immerse in the liquid Agilent 1290 Infinity II High Speed Pump User Manual 15 1 Introduction Leak and Waste Handling 16 Agilent 1290 Infinity Il High Speed Pump User Manual Agilent 1290 Infinity II High Speed Pump User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 18 Physical Specifications 21 Performance Specifications 22 This chapter provides information on environmental requirements physical and performance specifications ee Agilent Technologies 17 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Consideration The module power supply has wide ranging capabilities and accepts any line voltage in the range mentioned in Table 1 on page 21 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug
58. ased on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1290 Infinity lI High Speed Pump User Manual 179 10 Hardware Information Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the requ
59. ative e Parameter 1 4 referring to pump drive Probable cause 1 Wiper shifted 2 Pump drive spindle is defect Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 Pressure sensor calibration wrong or missing Error ID 22443 Pressure sensor calibration wrong or missing Parameter None Probable cause Suggested actions 1 Pressure sensor calibration wrong or e Replace pressure sensor missing e Please contact your Agilent service representative Seal wash pump was missing when tried to turn on Error ID 22499 The seal wash pump has not been detected while being configured or detected before Probable cause Suggested actions 1 Defect cable connection to seal wash pump Check cable connection 2 Defect seal wash pump motor Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1290 Infinity Il High Speed Pump User Manual 73 6 Error Information Pump Error Messages 74 Agilent 1290 Infinity II High Speed Pump User Manual Agilent 1290 Infinity Il High Speed Pump User Manual 7 Maintenance Introduction to Maintenance 77 Warnings and Cautions 79 Overview of Maintenance 81 Cleaning the Module 82 Install Fittings and Capillaries 83 Remove and Install Doors 84 Repl
60. cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Pump drive error Error ID 22438 22439 The pump drive failed during calibration Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive motor defect or mechanically Please contact your Agilent service blocked representative Agilent 1290 Infinity Il High Speed Pump User Manual 71 6 72 Error Information Pump drive stroke blocked Error ID 22441 During initialization the pump defines the operation position of the pump drives and therefore the pistons First the pump drive moves backwards to find a mechanical stop within the ball screw Afterwards pistons move forwards for finding the maximum available stroke volume These values are expected within a pre defined range Maximum stroke too short means that the outer drive position is too close This can be caused by a drive initialization without pump head or if the pump head has not been installed properly screws are loose e Parameter 1 4 referring to pump drive Probable cause 1 Wiper shifted 2 Pump head blocks piston movement 3 Pump drive motor is mechanically blocked Pump drive stop not found Error ID 22442 The maximum stroke is too long Suggested actions Please contact your Agilent service representative Replace clean or repair pump head Please contact your Agilent service represent
61. crews at the rear of the pump head The screws will be fixed tightly later 120 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 5 Insert the alignment piston of the pump head alignment CAUTION tool Lubricate the alignment piston with isopropanol or another suitable solvent before insertion Damage to the pump head R gt Using the alignment tool is mandatory Alignment piston gt Not using will break the pump head 6 Insert the pump head to the pump head alignment tool which is included to the 1290 Infinity Service Kit p n A 5067 4699 There are openings for the seal wash support ring and heat exchanger of the secondary pump head gt Observe the correct orientation of all parts mpa Pump head alignment tool rear al h i Pump head alignment tool front Agilent 1290 Infinity II High Speed Pump User Manual 121 7 Maintenance 7 Close the tool by closing the three screws at the connection ring Heat exchanger capillary CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 8 Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 5 Nm and fix the central alignment screw entra alignment screw 122 Agilent 1290 Infinity Il High Speed
62. ct the power cables and any external circuits before removing the instrument cover Do Not Modify the Instrument Do not install substitute parts or perform any unauthorized modification to the product Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained In Case of Damage WARNING Damage to the module Personal injury for example electrical shock intoxication gt Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel 214 Agilent 1290 Infinity lI High Speed Pump User Manual Appendix 12 Solvents WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as
63. d Please contact your Agilent service representative Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries Open Cover Error ID 0205 The top foam has been removed Probable cause Suggested actions 1 Foam not activating the sensor Please contact your Agilent service representative 2 Defective sensor or main board Please contact your Agilent service representative Agilent 1290 Infinity Il High Speed Pump User Manual 55 56 Error Information Cover Violation Error ID 7461 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed while the lamps are on or if an attempt is made to switch on for example the lamps with the foam removed the lamps are switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent s
64. d by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1290 Infinity Il High Speed Pump User Manual 169 10 Hardware Information Electrical Connections Serial Number Information The serial number information on the instrument labels provide the following information CCYWWSSSSS Format CC country of manufacturing DE Germany JP Japan CN China YWW year and week of last major manufacturing change e g 820 could be week 20 of 1998 or 2008 SSSSS real serial number Rear View of the Module APG Remote Power supply for externa CAN valves Configuration switch CAN Bus RS232 LAN Power plug Figure 18 Rear view of the High Speed Pump 170 Agilent 1290 Infinity Il High Speed Pump User Manual Interfaces Hardware Information 10 The Agilent 1200 Infinity Series II modules provide the following interfaces Table5 Agilent 1200 Infinity II Series Interfaces Module CAN USB LAN RS 232 Analog APG Special on board A ERI E Pumps G7104A Flexible Pump 2 No Yes Yes 1 G7120A High Speed Pump 2 No Yes Yes 1 A Samplers G7129A B ALS 2 Yes Yes No No E G7167A B Multisampler 2 Yes Yes No No E Detectors G7114A B VWD 2 Yes Yes No 1 E G7117A B DAD 2 Yes Yes No 1 E G7115A B DAD 2 Yes Yes No 1 E Others G7116B MCT 2 No No No No No Requires a HOST module via CAN The detector DAD MWD FLD VWD RID is the preferred access point for control via
65. e column The delay depends on the flow rate and the delay volume of the system In effect this means that in every HPLC system there is an additional isocratic segment in the gradient profile at the start of every run Usually the gradient profile is reported in terms of the mixture settings at the pump and the delay volume is not quoted even though this will have an effect on the chromatography This effect becomes more significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with narrow bore columns e g 2 1 mm i d as often used with mass spectrometric detection The delay volume in a system includes the volume in the pump from the point of mixing connections between pump and autosampler volume of the flow path through the autosampler and connections between autosampler and column 36 Agilent 1290 Infinity II High Speed Pump User Manual Optimizing Performance 4 How to Configure the Optimum Delay Volume The physical delay volume of the pump depends primarily on the use of the Jet Weaver mixer For UV detection the Jet Weaver should always be used but for mass spectrometric detection the user can decide to bypass the Jet Weaver in order to reduce the delay volume This only makes sense for ultra fast gradient operation less than 0 5 min or for use with very small volu
66. e cover First tighten these screws and then tighten the upper screws Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance Replace the Module Firmware When Tools required OR Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool 7 To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN USB FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module Agilent 1290 Infinity II High Speed Pump User Manual 135 7 Maintenance Prepare the Pump Module for Transport When
67. e front of the secondary pump head 2 Remove the front plate 3 Remove the screw at the bottom of the pump head Do not drop the golden spacer fitting Screw 4 Lift out the heat exchanger 114 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Pump Head Procedures 5 If removed first insert the spacer fitting Then insert the 6 Use the 19 mm screw for fixing the front plate new heat exchanger to the opening in the pump head and lift it over the pins Front plate es Spacer fitting 7 Insert and fix the screw Screw Agilent 1290 Infinity I High Speed Pump User Manual 115 7 Maintenance Replace Wash Seal and Gasket Tools required p n Description 01018 23702 Insert tool Parts required p n Description 0905 1718 Wash Seal PE 5062 2484 Gasket seal wash pack of 6 1 If the wash seal shall be replaced by a new one use the 2 Use the soft plastic side of the insert tool for inserting the steel side of the insert tool for removing it wash seal into the support ring 8 Insert tool Wash seal Wash seal CES 7 Support ring Clean seals before reinstallation or use new ones Store seals in isopropanol bath before installation 116 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 3 Put the gasket into the support ring and insert the seal holder Distance sh
68. e range and frequency of your power distribution matches to the power specification of the individual instrument gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations gt Make all connections to the unit before applying power Note the instrument s external markings described under Safety Symbols on page 216 Ground the Instrument WARNING Missing electrical ground Electrical shock gt If your product is provided with a grounding type power plug the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard gt The ground pin must be firmly connected to an electrical ground safety ground terminal at the power outlet Any interruption of the protective grounding conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury Agilent 1290 Infinity Il High Speed Pump User Manual 213 12 Appendix Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes Explosion hazard gt Do not operate the instrument in the presence of flammable gases or fumes Do Not Remove the Instrument Cover WARNING Instrument covers removed Electrical shock gt Do Not Remove the Instrument Cover gt Only Agilent authorized personnel are allowed to remove instrument covers Always disconne
69. ect the power cable to the Instrument while the covers are removed Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas The electronics of the module will not allow operation of the module when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the fan immediately Voltages for the other electronic components will be turned off after 30 seconds The status lamp will light up red and an error will be logged into the logbook of the user interface Always operate the module with the top covers in place Agilent 1290 Infinity Il High Speed Pump User Manual 79 7 Maintenance Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards
70. eet Seal holder Gasket Support ring Lor Agilent 1290 Infinity II High Speed Pump User Manual 117 7 Maintenance Assemble the Primary and Secondary Pump Head When Tools required Parts required CAUTION This procedure describes how to assemble the secondary pump head using the pump head alignment tool Assembling the primary pump head can be done accordingly The secondary pump head has the heat exchanger capillary which must fit into the openings of the alignment tool whereas the primary pump head does not have a heat exchanger Before installing the pump head p n Description Pump head alignment tool G4220 20012 Torque wrench 2 25 Nm G4220 20013 4 mm hex bit G4220 20041 Bit Torx 10x25 mm G4220 20015 Adapter in square to hex 01018 23702 Insert tool p n Description 0905 1719 PE Seal See chapter Parts for details Damage of the pump piston The pump piston is very sensitive to shearing forces from the side gt Use the alignment piston of the pump head alignment tool for the alignment procedure described below CAUTION Wrong orientation of pins on support ring Assembling the pump head without paying attention to the correct orientation of the pins on the support ring can lead to leaks or damage of the piston and pump head gt Observe pins on the support ring which help assembling the parts of the pump head in the correct orientation Agilent 1290 Infinity Il High Speed Pump U
71. efault When Using Default is selected the factory default parameters are taken instead These parameters enable a TCP IP connection to the LAN interface without further configuration see Table 13 on page 187 Default Active Parameter Parameter Figure 27 Using Default Principle Using the default address in your local area network may result in network problems Take care and change it to a valid address immediately Table 13 Using Default Parameters IP address 192 168 254 11 Subnet Mask 255 255 255 0 Default Gateway not specified Agilent 1290 Infinity Il High Speed Pump User Manual 187 11 LAN Configuration 188 Since the default IP address is a so called local address it will not be routed by any network device Thus the PC and the module must reside in the same subnet The user may open a Telnet session using the default IP address and change the parameters stored in the non volatile memory of the module He may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection In the Using Default mode the parameters stored in the memory of the module are not cleared automatically If not changed by the user the
72. entions Agilent 1290 Infinity Il High Speed Pump User Manual 189 11 LAN Configuration Setup DHCP Software required LONTIQ ation Frotocol L The modules in the stack must have at least firmware from set A 06 34 and the above mentioned modules B 06 40 or above must from the same firmware set 1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Instant Pilot the MAC address can be found under Details in the LAN section System Info Controller DE12345678 G4208A Main Revision DAD EX RPA Cn 64260019 G1315D Main Revision _ Resident Revison B 06 41 0002 B 06 40 0007 On time 3d 01 33h Installed Options Dhcp LAN TCP IP Mode DHCP LAN TCP IP Address 130 168 132 219 0030D314F89E TYPE G61315 66565 SER MAC REV AC MFG 2140 0820 848728 no info v information on each module S 10 08 Figure 29 190 LAN Setting on Instant Pilot Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or the main board of above mentioned modules Table 14 G1369C LAN Interface Card configuration switch on the card SW 4 SW5 SW 6 SW7 SW 8 Initialization Mode ON OFF OFF OFF OFF DHCP Table 1
73. er Manual 87 7 Maintenance Replace the Pressure Sensor 3 Carefully pull out the pressure sensor for about 2 cm 4 Connect the new pressure sensor to the pressure sensor Then unscrew the cable from the pressure sensor connector amp 5 Fix the pressure sensor to the instrument chassis 6 Connect the capillaries from the valve to the pressure sensor connect port 3 to the pressure sensor inlet and port 2 to the outlet 88 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Overview of Torques for Pump Head Procedures Overview of Torques for Pump Head Procedures Figure 6 on page 89 gives an overview of the torques that need to be set during maintenance of the pump heads Figure 6 Overview of torques for pump head procedures Agilent 1290 Infinity Il High Speed Pump User Manual 89 7 Maintenance Replace the Inlet Valve Replace the Inlet Valve When If Inlet valve is defective Tools required p n Description Wrench 14 mm G4220 20012 Torque wrench 2 25 Nm Parts required p n Description G4220 60022 Inlet valve primary pump head NOTE For best performance and life time and for avoiding leaks use a torque wrench set to 10 Nm for fixing the inlet valve 1 Close the shut off valves to avoid solvent leaks 2 Unscrew the tubing at the inlet valve 90 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Replace the In
74. er rollover Error ID 22424 Invalid pump drive encoder signals have been detected Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Drive position limit Error ID 22425 Internal error Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Internal error Please contact your Agilent service representative Insufficient power of drive encoder LED Error ID 22426 Insufficient power of drive encoder LED Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative 68 Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 Drive encoder error Error ID 22427 22430 An error has occurred for the pump drive encoder Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Pump drive encoder is defect Please contact your Agilent service representative Writing the purge valve tag failed Error ID 22431 Writing the purge valve tag failed e Parameter None Probable cause Suggested actions 1 Purge valve head tag defect Replace purge valve head 2 Purge valve tag reader is defect Please contact your Agilent service representative Agilent 1290 Infinity Il High Speed Pump User Manual 69 Error Information Error ID 22433 Current
75. ervice operation representative 2 Foam not activating the sensor Please contact your Agilent service representative Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 Pump Error Messages These errors are pump specific Pressure of binary pump above upper limit Error ID 22014 The pressure has exceeded the upper pressure limit e Parameter Measured pressure Probable cause Suggested actions 1 Blockage in flow path after the pressure Check for blockages in the LC system e g sensor purge valve Jet Weaver degraded column column frits needle needle seat capillaries etc Check for particles in the solvent 2 Inappropriate settings pressure limit flow Decrease flow rate rate e Increase pressure limit Agilent 1290 Infinity Il High Speed Pump User Manual 57 6 Error Information Pressure below lower limit Error ID 22015 The pressure has dropped below the lower limit Parameter None Probable cause Suggested actions 1 Leak Check for leaks 2 Bottle empty Check bottle filling 3 Wrong solvent viscosity Check solvent 4 Inappropriate setting Check flow rate and lower pressure limit 5 Column degradation Replace column Target pressure not reached for binary pump degasser Error ID 22031 The target pressure of the binary pump degasser has not been reached within the expected time Parameter Pressure in mbar Probable cause Suggested actions 1 Conden
76. fter re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 6976 1 168 Agilent 1290 Infinity Il High Speed Pump User Manual Hardware Information 10 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplie
77. ge 21 G gasket replace 116 general error messages 50 heat exchanger replace 113 high pressure filter assembly replace 132 humidity 21 initialization mode selection 185 inlet valve Agilent 1290 Infinity I High Speed Pump User Manual Index release 97 replace 90 stuck 97 installation bench space 20 power considerations 18 site requirements 17 install doors 84 valve rail kit 134 instrument layout 180 insufficient power of drive encoder LED 68 interfaces 44 Infinity Il 171 internet 221 J jet weaver change configuration 95 replace 95 L LAN automatic configuration with Bootp 193 Bootp amp Store 186 Bootp 185 cable 162 configuration switche 184 configuration 181 first steps 182 initialization mode selection 185 link configuration selection 192 manual configuration with telnet 204 manual configuration 203 PC and User Interface Software Setup 208 TCP IP parameter configuration 183 223 Index using default 187 using stored 187 leak sensor open 53 leak sensor short 52 leak 55 line frequency 21 line voltage 21 link configuration selection 192 lost CAN partner 52 M MAC address determine 198 MAC address 182 maintenance feedback 179 introduction 77 replacing firmware 135 manual configuration of LAN 203 message cover violation 56 ignition without cover 55 55 remote timeout 51 module firmware replace 135 non operating altitude 21 non operating temperature 21 no
78. gilent 1290 Infinity II High Speed Pump User Manual Agilent 1290 Infinity Il High Speed Pump User Manual 6 Error Information What Are ErrorMessages 49 General Error Messages 50 Timeout 50 Shutdown 50 Remote Timeout 51 Lost CAN Partner 52 Leak Sensor Short 52 Leak Sensor Open 53 Compensation Sensor Open 53 Compensation Sensor Short 54 Fan Failed 54 Leak 55 Open Cover 55 Cover Violation 56 Pump Error Messages 5 7 Pressure of binary pump above upper limit 57 Pressure below lower limit 58 Target pressure not reached for binary pump degasser 58 Degasser s pressure limit violation 59 Solvent counter exceeded limit 59 Waste counter limit exceeded 60 Flow rate limit exceeded 60 Binary pump shutdown during analysis 61 Reading the pump encoder tag failed 61 Writing the pump encoder tag failed 62 Pump drive blocked or encoder failed 62 Drive current too low 63 Drive Encoder failed 63 Brae Agilent Technologies 47 48 Error Information Drive current too high 64 Drive timeout 64 Overcurrent of pump drive 65 Overcurrent of solvent selection valve SSV 65 Deliver underrun 65 Defect connection between main board and pump drive encoder 66 Pump drive encoder defect 66 Purge valve failed 67 Reading of purge valve tag failed 67 Pump drive encoder rollover 68 Drive position limit 68 Insufficient power of drive encoder LED 68 Drive encoder error 69 Writing the purge valve tag failed 69 Current of primary pump drive too hig
79. h 70 Current of secondary pump drive too high 70 Unknown purge valve type 71 Pump drive encoder error 71 Pump drive error 71 Pump drive stroke blocked 72 Pump drive stop notfound 72 Pressure sensor calibration wrong or missing 73 Seal wash pump was missing when tried to turnon 73 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been
80. h Sensor Leak Tube Left Pump Head Assembl Purge Valve Right Pump Head Assembly Degasser Pressure Sensor Figure 4 Maintenance parts Figure 5 on page 78 shows the flow connections between these main assemblies Agilent 1290 Infinity Il High Speed Pump User Manual 71 7 Maintenance Introduction to Maintenance Multisampler ds hal ik Seal wash pump Ssv l Pump head A e Seal wash sensor Jet Weaver aa Pump head B Degassing unit High Speed Pump Figure 5 The hydraulic path Recommended Interval for Preventive Maintenance The recommended interval for preventive maintenance is e 100 L or 1 year whichever comes first This recommendation is valid for LC instruments on which typical applications are running A typical application can be characterized as follows e pressure range 100 800 bar e flow rates 0 5 3 5 mL min e typical solvents used in reversed phase LC 78 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Warnings and Cautions Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not conn
81. he Agilent BootP Service Setup screen indicates completion 14 Click Finish to exit the Agilent BootP Service Setup screen 15 Remove the DVD from the drive This completes installation 16 Start BootP Service in the Windows services On the Windows desktop click right on Computer icon select Manage gt Services and Applications gt Services Select the Agilent BootP Service and click Start 196 Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 Two Methods to Determine the MAC Address Enabling logging to discover the MAC address using BootP If you want to see the MAC address select the Do you want to log BootP requests check box 1 Open BootP Settings from Start gt All Programs gt Agilent BootP Service gt EditBootPSettings 2 In BootP Settings check Do you want to log BootP requests to enable logging Logging MV Do you want to log bootP requests BootP Log File Ea EP Figure 31 Enable BootP logging The log file is located in C Documents and Settings All Users Application Data Agilent BootP LogFile It contains a MAC address entry for each device that requests configuration information from BootP 3 Click OK to save the values or Cancel to discard them The editing ends 4 After each modification of the BootP settings i e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service
82. hipment box Agilent 1290 Infinity Il High Speed Pump User Manual Agilent 1290 Infinity II High Speed Pump User Manual 8 Parts and Materials for Maintenance Overview of Maintenance Parts 138 Flow Connections 139 Pump Head Assemblies 140 Pump Head Assembly Parts 141 Primary Pump Head Parts 142 Secondary Pump Head Parts 144 Purge Valve 146 Cover Parts 147 Leak Parts 147 Accessory Kit 148 Tools 149 HPLC System Tool Kit 151 This chapter provides information on parts for maintenance Apg Agilent Technologies 137 8 Parts and Materials for Maintenance Overview of Maintenance Parts Overview of Maintenance Parts Figure 7 Item 1 nN oo on A WO N 138 Overview of main assemblies p n G4220 60027 G4220 60012 G7120 60029 5065 4445 G7104 60001 thd G4220 60900 G4220 60910 Description Jet Weaver 35 pL 100 pL Jet Weaver 380 uL OPTIONAL SSV Valve Assembly Peristaltic pump with Pharmed tubing Pressure sensor 1300 bar Purge valve head Pump Head Channel A with Seal Wash Pump Head Channel B with Seal Wash Agilent 1290 Infinity Il High Speed Pump User Manual Flow Connections Parts and Materials for Maintenance 8 Flow Connections Figure 8 Flow connections of the High Speed Pump Item p n 1 2 5067 4655 2 1 5065 4445 3 5 1 5065 9978 4 1 G4220 60035 6 1 5067 4661 7 1 G4220 87000 8 2 01090 87308 G7120 40004 5067 4124 G7120 60007 G7120 68070 i l 1 G422
83. ication interface 1 Note the MAC Media Access Control address for further reference The MAC or hardware address of the LAN interfaces is a world wide unique identifier No other network device will have the same hardware address The MAC address can be found on a label at the rear of the module see Figure 22 on page 182 G42208 65800 MIR AR Part number of the pump main board B 01 XX 0925 Revision Code Vendor Year and Week of assembly MAC 000001000001 MAC address Made in Germany Country of Origin Figure 21 MAC Label 2 Connect the instrument s LAN interface see Figure 22 on page 182 to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable MAC label dJe Dio LAN interface e228 aja Mm Ue e 0 0 MNG bg 0 000000800BA 8800000000 6000 Is ne 0 0800000000800080 M 0 0 68 00 00 D gt cD lt D ED aD i ma jaw DPDT D DOD p Sa CD BO cD a T wc cme CDD OD i oo 6908 uo i 000 080000000 j Me Q O Z aD SS w i J00000 t it 182 Agilent 1290 Infinity II High Speed Pump User Manual LAN Configuration 11 TCP IP parameter configuration To operate properly in a network environment the LAN interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask Default Gateway The TCP IP parameters
84. ight 200 Side Cover Left 200 Door assy 200 left IF II Door assy 200 right IF II Description Leak Adapter Agilent 1290 Infinity lI High Speed Pump User Manual 147 8 Parts and Materials for Maintenance Accessory Kit Accessory kit G7120 68705 contains the following parts p n 0100 1816 5065 9978 0890 2207 5043 1013 5067 4124 5067 4670 5067 6129 5181 1519 5500 1155 9301 1337 9301 6476 G4220 60035 G7120 40004 5063 6527 5500 1156 5500 1169 148 Description Fitting Waste Tube to Purge Valve Tubing 1 mm i d 3 mm o d silicone 5 m Tubing Sleeving Flex Tubing Clip Shutoff valve SST cap 0 17 mm ID 600 mm pre swaged Capillary ST 0 17 x 300 mm S SX CAN cable Agilent module to module 1 m Tube Connector 90 degree ID 6 4 Syringe adapter Syringe with luerlock 5 mL Polypropylene Tubing kit 140 mm 2 pk Valve Holder Left Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste T Tube Connector ID6 4 Y Tube Connector ID6 4 Agilent 1290 Infinity Il High Speed Pump User Manual Parts and Materials for Maintenance 8 Tools Item p n Description 5067 4699 1290 Infinity pump service kit 1 5067 5688 Torque wrench 1 25 Nm with 14 mm wrench 2 G4220 20013 4 mm hex bit 3 G4220 20014 2 5 mm Hex Bit 4 G4220 20015 Adapter in square to hex 5 G4220 20041 Bit Torx 10x25 mm 5067 5691 Wrench open 19 mm 5023 0285 Replacement kit for 1290 Infinity pump head alignment tool piston handle 1290 Infinity
85. ine to achieve high instrument and analytical efficiency Status indicator Jet weaver Seal wash pump Pump head A Pump head B Pressure sensor Leak drain Power switch Figure 1 Overview of the High Speed Pump 10 Agilent 1290 Infinity Il High Speed Pump User Manual Introduction 1 Pump Principle The 1290 Inifinity II Highspeed Pump features a dual pump head design for generation of binary gradients A solvent selection valve allows to choose from two solvents per pump head However this valve cannot the switched during a run Each pump head is equpipped with two independently actuated pistons in series Delivery cycle 1 Piston two moves forward to deliver solvent into the flow path The flow rate is thereby determined by the speed of the piston At the same time piston one draws aspirates solvent from the solvent reservoir The two piston chambers are isolated by a check valve outlet ball valve 2 Shortly before the end of the delivery stroke of piston two piston one reverses its direction The check valve passive inlet valve at the inlet to piston chamber one closes while the solvent in piston chamber one gets re compresed to system operating pressure 3 Piston two reverses while piston one delivery the set flow rate into the flow path and re fills piston chamber two 4 When piston two has reached that end of its intake stroke it reverses and the delivery cycle starts again
86. ing potential GWP see instrument label Confirms that a manufactured product complies with all applicable European Community directives The European Declaration of Conformity is available at http regulations corporate agilent com DoC search htm Manufacturing date Power symbol indicates On Off The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position Pacemaker Magnets could affect the functioning of pacemakers and implanted heart defibrillators A pacemaker could switch into test mode and cause illness A heart defibrillator may stop working If you wear these devices keep at least 55 mm distance to magnets Warn others who wear these devices from getting too close to magnets Agilent 1290 Infinity II High Speed Pump User Manual Appendix 12 Table 18 Symbols Magnetic field uN Magnets produce a far reaching strong magnetic field They could damage TVs and laptops computer hard drives credit and ATM cards data storage media mechanical watches hearing aids and speakers Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields Indicates a pinching or crushing hazard Q Indicates a piercing or cutting hazard WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CA
87. ion protocol and instrument specific initialization procedures All modules with on board LAN e Default is ALL switches DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 e For specific LAN modes switches 3 8 must be set as required e For boot test modes switches 1 2 must be UP plus required mode For normal operation use the default best settings SX Agilent Tecdnsiogies VUeUUUYUYUY 176 Figure 20 Location of Configuration Switch example shows a G4212A DAD Agilent 1290 Infinity Il High Speed Pump User Manual Hardware Information 10 To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration Table 8 8 bit Configuration Switch with on board LAN Mode Function sW 1 SW 2 SW 3 SW 4 SW 5 SW6 SW7 SW 8 LAN 0 0 Link Configuration Init Mode Selection Auto negotiation 0 X X Xx xX X 10 MBit half duplex 1 0 0 x X X 10 MBit full duplex 1 0 1 x xX X 100 MBit half duplex 1 1 0 x xX X 100 MBit full duplex 1 1 1 x X x Bootp x Xx Xx 0 0 0 Bootp amp Store x x x 0 0 1 Using Stored x Xx Xx 0 1 0 DHCP Xx xX xX 1 0 0 Using Default x Xx Xx 0 1 1 TEST 1 1 System NVRAM Boot Resident System 1 x Revert to Default Data Coldstart x Xx x 1 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN set
88. ions Disassembling the flow connection between the two pump heads of the pump head assembly solvent channel can damage the connection and cause leaks gt Do not disconnect the flow connection between the pump heads CAUTION Damage of internal parts gt Do not apply a strong force to the screws of the pump head gt Use a torque hex key for that purpose CAUTION Damage of the pump piston Removing pump heads in a position other than the maintenance position can damage the pump piston gt Before switching off the pump bring it to the maintenance position CAUTION Damage of pump drives The pump drive can be damaged if the pump initializes after switching it on without having the pump head installed properly gt Use the Lab Advisor maintenance procedure for replacing pump heads gt Install the pump head correctly before switching on the pump Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 VTE Tm Damaged pump head Disassembling or reassembling the pump head with tools other than the ones recommended can damage pump heads and significantly reduce their life time gt Follow all instructions step by step gt Use recommended tools like the pump head alignment tool and a torque wrench CAUTION Damage of pump piston The pump piston is made of Zr07 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt
89. iption Figure 33 Windows Services screen 2 Right click Agilent BootP Service 3 Select Stop 4 Close the Services and Administrative Tools screen Agilent 1290 Infinity II High Speed Pump User Manual 201 11 LAN Configuration Editing the IP address and other parameters in EditBootPSettings 1 Select Start gt All Programs gt Agilent BootP Service and select Edit BootP Settings The BootP Settings screen appears 2 When the BootP Settings screen is first opened it shows the default settings from installation 3 Press Edit BootP Addresses to edit the Tab File x Host Name IP Address Comment Gateway 006000111599 Agier LC1 1011 10 Agiert LET ngtt 285 255 2550 0000 005000222883 Ager C2 101 1 1 102 Agiert LC2 left 252552550 0000 Figure 34 Edit BootP Adresses screen 4 In the Edit BootP Addresses screen press Add to create a new entry or select an existing line from the table and press Modify or Delete to change the IP address comment subnet mask for example in the Tab File If you change the IP address it will be necessary to power cycle the instrument for the changes to take effect 5 Leave Edit BootP Addresses by pressing Close 6 Exit BootP Settings by pressing OK Restarting the Agilent BootP Service 1 In the Windows control panel select Administrative Tools gt Services The Services screen appears see Figure 33 on page 201 2 Right click Agilent BootP Service and select Start
90. ired locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 180 Agilent 1290 Infinity Il High Speed Pump User Manual Agilent 1290 Infinity Il High Speed Pump User Manual 11 LAN Configuration What You Have to Do First 182 TCP IP parameter configuration 183 Configuration Switch 184 Initialization mode selection 185 Dynamic Host Configuration Protocol DHCP 189 General Information DHCP 189 Setup DHCP 190 Link configuration selection 192 Automatic configuration with Bootp 193 About Agilent BootP Service 193 How BootP Service Works 194 Situation Cannot Establish LAN Communication 194 Installation of BootP Service 195 Two Methods to Determine the MAC Address 197 Assigning IP Addresses Using the Agilent BootP Service 198 Changing the IP Address of an Instrument Using the Agilent BootP Service 201 Manual Configuration 203 With Telnet 204 PC and User Interface Software Setup Setup 208 PC Setup for Local Configuration 208 User Interface Software Setup 209 This chapter provides information on connecting the module to the Agilent ChemStation PC oh Agilent Technologies 181 11 LAN Configuration What You Have to Do First What You Have to Do First The module has an on board LAN commun
91. lent 1290 Infinity Il High Speed Pump User Manual This chapter provides information on cables used with the modules Agilent 1290 Infinity II High Speed Pump User Manual 9 Identifying Cables Cable Overview 154 Analog Cables 156 Remote Cables 158 CAN LAN Cables 162 Agilent Module to PC 163 USB 164 Apg Agilent Technologies 153 9 Identifying Cables Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 01046 60105 Remote cables p n 5188 8029 5188 8044 5188 8045 5061 3378 01046 60201 CAN cables p n 5181 1516 5181 1519 154 Description Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description ERI to general purpose Remote Cable ERI ERI Remote Cable APG ERI Remote Cable to 35900 A D converter Agilent module to general purpose Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Agilent 1290 Infinity Il High Speed Pump User Manual LAN cables p n 5023 0203 5023 0202 RS 232 cables not for FUSION p n board G1530 60600 RS232 61601 5181 1561 USB cables p n 5188 8050 5188 8049 Identifying Cables 9 Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for
92. let Valve 3 With a 14 mm wrench unscrew the inlet valve and 4 Install inlet valve and tighten it at 10 Nm with a torque remove it wrench 14 mm 5 Attach the inlet tubing at the inlet valve 6 Open the shut off valves and purge the system to remove j air Agilent 1290 Infinity II High Speed Pump User Manual 91 7 Maintenance Replace the Outlet Valve Replace the Outlet Valve When If Outlet valve is defective Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2603 G4220 20012 64220 20041 Parts required p n G4220 60028 64220 20020 Spanner double open ended 12X14 mm Chrome Torque wrench 2 25 Nm Bit Torx 10x25 mm Description Outlet valve primary pump head Internal gold seal for Outlet Valve 1 Close the shut off valves to avoid solvent leaks 2 Remove the cap from the outlet valve 92 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Replace the Outlet Valve 3 Open the 2 5 mm hex screw at the top of the primary pump head which fixes the connection capillary of the heat exchanger and remove it 1 Then lift up the capillary and remove it from the primary pump head 2 4 A gold seal between outlet valve and heat exchanger capillary is used for a tight connection The seal can be replaced separately as needed 6 Insert the outlet valve into the pump head 1 Using a torque wrench set 1
93. mal Phase Applications 32 This chapter explains the operational parameters of the Agilent 1290 Infinity II High Speed Pump ot Agilent Technologies 25 Using the Pump Magnets 3 Magnets 1 This stack exemplarily shows the magnets positions in the modules Agilent 1290 Infinity II High Speed Pump User Manual 26 Using the Pump 3 Turn on off Turn on off Power switch 1 On 2 Off Agilent 1290 Infinity Il High Speed Pump User Manual 27 3 Using the Pump Status Indicators 1 The module status indicator indicates one of six possible module conditions Status indicators 1 Idle 2 Run mode 3 Not ready Waiting for a specific pre run condition to be reached or completed 4 Error mode interrupts the analysis and requires attention for example a leak or defective internal components 5 Resident mode blinking for example during update of main firmware 6 Bootloader mode fast blinking Try to re boot the module or try a cold start Then try a firmware update 28 Agilent 1290 Infinity II High Speed Pump User Manual Using the Pump 3 Best Practices Daily Weekly tasks Daily tasks e Replace mobile phase based on water buffer e Replace organic mobile phase latest every 2nd day e Check seal wash solvent e Run conditioning with composition of your application Weekly tasks e Change seal wash solvent 10 90 isopropanol water and bot
94. me columns If the Jet Weaver is bypassed the connection tubing to the autosampler is routed directly from the purge valve Before disconnecting a Jet Weaver from the flow path flush it with organic solvent Avoid leaving water or buffers inside the Jet Weaver which may cause the growth of microorganisms like algae or bacteria Sometimes it may be advisable to increase the delay volume in the pump Specifically this can be the case when UV detection is employed and a strongly UV absorbing compound has been added to the mobile phase This can have the effect of emphasizing any pump noise and the most common example is the use of trifluoroacetic acid TFA in the analysis of proteins and peptides The effect can be mitigated by increasing the mixer volume The following different Jet Weaver configurations are available The Jet Weaver 35 uL 100 uL G4220 60027 has two alternative volumes in the same unit The switch from the lower volume 35 ul to the higher volume 100 ul is done by uninstalling it turning it around from front to back and re installing it see Change Configuration or Replace the Jet Weaver on page 95 The mixing volume and hence delay volume is increased by 65 ul and the baseline performance with additives like TFA will be improved The configuration of the Jet Weaver is logged automatically by an attached RFID tag The 380 uL Jet Weaver high performance mixer is optionally available for demanding applicati
95. me emg a aaen s atte Agilent Technologies Notices Agilent Technologies Inc 2014 2015 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 67120 90000 Rev D Edition 07 2015 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software
96. n The pump piston is made of Zr07 based ceramic which is a very hard and resistant material but it is sensitive to shearing forces from the side gt Do not try to remove the pump piston from the rear gt Do not use the piston for removing pump seals 1 For disassembling the pump head remove the 4 hex 2 Remove the front part of the pump head including pump screws at the rear of the pump head chamber housing distance sheet pump seal and seal holder and support ring with wash seal and gasket upport ring S gt Seal wash gasket gt LNA istance sheet s A W Pump chamber housing Agilent 1290 Infinity II High Speed Pump User Manual 109 7 Maintenance 3 Remove the piston from the piston housing by pushing it to the rear then pull it out from the rear 110 4 Check the pump pistons for scratches grooves and dents when changing the piston seals Damaged pistons cause micro leaks and will decrease the lifetime of the seals Piston surface Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 5 Use the steel side of the insert tool for removing the pump seal from the pump chamber housing AT Insert tool Pump seal Do not use the pump piston for that purpose as this can break it Agilent 1290 Infinity II High Speed Pump User Manual 6 To flush out particles from the pump head use pressurized air 1 2 and 3
97. nd acids at or below pH 7 gt Do not use normal phase valves in applications running with aqueous solutions 32 The N Valves have been tested successfully in using hexane at pressures below 100 bar heptane can be used as a substitute for neurotoxic hexane Agilent 1290 Infinity II High Speed Pump User Manual Using the Pump 3 Seals for Normal Phase Applications For running normal phase on 1200 Infinity Series pumps yellow PE seals are required which exist as piston seals and wash seals Seal wash is very uncommon for normal phase applications no buffers needed but wash seals are needed for seal wash pump heads 1290 Infinity pumps use PE seals by default In combination with ceramic pistons PE seals are used for both reversed phase 1200 bar and normal phase applications 1260 Infinity pumps use sapphire pistons and black PTFE piston and wash seals by default 600 bar Such PTFE seals create small wear particles in normal phase applications which can clog valves and other parts in the flow path PE seals have a limited life time when used with normal phase solvents and sapphire pistons Agilent recommends a maximum pressure of 200 bar for this combination which shall also be applied for pressure tests Tetrahydrofuran THF is not compatible with PE seals Black PTFE seals should be used with THF even in 1290 Infinity pumps In this case use a maximum pressure of about 400 bar Choice of Normal Phase Val
98. nectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message 50 Agilent 1290 Infinity II High Speed Pump User Manual Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Remote Timeout Error ID 0070 Error Information 6 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Agilent 1290 Infinity Il High Speed Pump User Manual Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for anal
99. nologies on Internet 221 This chapter provides addition information on safety legal and web ae Agilent Technologies 211 12 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards General Do not use this product in any manner not specified by the manufacturer The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions 212 Agilent 1290 Infinity Il High Speed Pump User Manual Appendix 12 Before Applying Power WARNING Wrong voltage range frequency or cabling Personal injury or damage to the instrument gt Verify that the voltag
100. of primary pump drive too high The current of the primary pump drive is too high e Parameter 1 or 4 referring to pump drive Probable cause 1 Blockage of flow path between primary pump head and pressure sensor e g of the heat exchanger 2 Primary pump drive is defect Error ID 22434 Suggested actions Check for blockages in flow path e Please contact your Agilent service representative Please contact your Agilent service representative Current of secondary pump drive too high The current of the secondary pump drive is too high e Parameter 2 or 3 referring to pump drive Probable cause 1 Blockage of flow path between secondary pump head and pressure sensor e g of the heat exchanger 2 Secondary pump drive is defect Suggested actions Check for blockages in the flow path e Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1290 Infinity II High Speed Pump User Manual Error Information Unknown purge valve type Error ID 22435 The type information of the purge valve is invalid Parameter None Probable cause Suggested actions 1 Wrong valve head installed Check or replace purge valve head 2 Valve head has invalid RFID tag content Check or replace purge valve head Pump drive encoder error Error ID 22437 The pump drive encoder has generated no signal Parameter 1 4 referring to pump drive Probable
101. olution N plate count measure of column efficiency a selectivity between two peaks e ko retention factor of second peak formerly called capacity factor The term that has the most significant effect on resolution is the selectivity a and practically varying this term involves changing the type of stationary phase C18 C8 phenyl nitrile etc the mobile phase and temperature to maximize the selectivity differences between the solutes to be separated This is a substantial piece of work which is best done with an automated method development system which allows a wide range of conditions on different columns and mobile phases to be assessed in an ordered scouting protocol This section considers how to get higher resolution with any chosen stationary and mobile phases If an automated method development system was used in the decision on phases it is likely that short columns were used for fast analysis in each step of the scouting Agilent 1290 Infinity II High Speed Pump User Manual 39 4 40 Optimizing Performance The resolution equation shows that the next most significant term is the plate count or efficiency N and this can be optimized in a number of ways N is inversely proportional to the particle size and directly proportional to the length of a column and so smaller particle size and a longer column will give a higher plate number The pressure rises with the inverse square of the particle size and proportionally
102. on selection 192 Automatic configuration with Bootp 193 Manual Configuration 203 PC and User Interface Software Setup Setup 208 12 Appendix 211 General Safety Information 212 Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 218 Radio Interference 219 Sound Emission 220 Agilent Technologies on Internet 221 Agilent 1290 Infinity lI High Speed Pump User Manual Contents 8 Agilent 1290 Infinity II High Speed Pump User Manual Agilent 1290 Infinity II High Speed Pump User Manual e ee 1 r Introduction Product Description 10 Pump Principle 11 Leak and Waste Handling 13 Waste Concept 15 This chapter gives an introduction to the pump and an instrument overview ott Agilent Technologies 1 Introduction Product Description Product Description The Agilent 1290 Infinity II High Speed Pump can enhance your efficiency through high speed and chromatographic performance A low delay volume mixer allows you to run fast gradients for narrow bore applications for high laboratory efficiency The new 1290 Infinity II LC power range has a high instrument efficiency allowing you to run any HPLC and UHPLC method The full ISET range enables you to transfer existing methods from different instruments including current Agilent systems as well as instruments from other manufacturers Active damping automatic purge valve new ultralow dispersion kits or low delay volume capability comb
103. ons which use solvents in different channels for example A versus B that differ strongly in their UV Vis absorption for example by using trifluoroacetic acid TFA as a modifier which has a high absorbance Agilent 1290 Infinity Il High Speed Pump User Manual 37 4 Optimizing Performance Solvent packages created by the pump may persist until the solvent reaches the detector flow cell Absorption fluctuations can then show up as baseline noise also referred to as mixing noise Applications like impurity quantitation or lowest level compound detection require minimizing this noise The 380 uL Jet Weaver strongly improves mixing and therefore reduces baseline noise and improves sensitivity in detection Patented Agilent microfluidic technology offers high mixing performance at a low internal volume of 380 uL 38 Agilent 1290 Infinity II High Speed Pump User Manual Optimizing Performance 4 How to Achieve Higher Resolution Increased resolution in a separation will improve the qualitative and quantitative data analysis allow more peaks to be separated or offer further scope for speeding up the separation This section explains how resolution can be increased by examining the following points e Optimize selectivity e Smaller particle size packing Longer Columns e Shallower gradients faster flow Resolution between two peaks is described by the resolution equation 1 a 1 k Rs VN 2 i 4 a k where R res
104. point to point connection Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Description USB A M USB Mini B 3 m PC Module USB A F USB Mini B M OTG Module to Flash Drive Agilent 1290 Infinity lI High Speed Pump User Manual 155 9 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 35900 A D converters p n 35900 60750 35900 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog 156 Agilent 1290 Infinity lI High Speed Pump User Manual Agilent Module to BNC Connector Identifying Cables 9 p n 8120 1840 Pin BNC Pin Agilent Signal Name module a O Shield Shield Analog SEEN VIZ Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog Analog Se 3 Red i eo Agilent 1290 Infinity lI High Speed Pump User Manual 157 9 Identifying Cables Remote Cables ERI Enhanced Remote Interface 5188 8029 ER
105. que consult the manual of your torque wrench 2 Insert the heat exchanger capillary into the outlet of the primary pump head 1 Using a torque key which is included to the 1290 Infinity Service Kit p n 5067 4699 set 3 Nm and close the screw at the top of the outlet 2 2 SE Agilent 1290 Infinity II High Speed Pump User Manual 127 7 Maintenance Pump Head Procedures Install the Pump Head Assembly 1 Bring the pump drive to the maintenance position using CAUTION the Lab Advisor user interface Go to Tools gt CAUTION Remove Install Pump Head and follow instructions Damage to the pump head given on the screen Both pump drives must be retracted Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 2 Install the new pump head assembly by tightening the screws step by step Apply 5 Nm using a torque hex key which is included to the 1290 Infinity Service Kit p n 5067 4699 128 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 Pump Head Procedures 3 Connect the degassing unit outlet to the inlet of the primary pump head 4 Connect the outlet of the secondary pump head to the inlet of the purge valve NOTE Channel A left pump head assembly is connected to port 4 channel B right pump head assembly to port 1 of the purge valve
106. quest the user can send this report to Agilent for a significantly improved troubleshooting and repair process The Agilent Lab Advisor software is available in two versions e Lab Advisor Basic Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Infinity II Series pump The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Review Client included in Lab Advisor Advanced allows to load and examine the uploaded data no matter on which instrument it was generated This makes Data Sharing an ideal tool for internal support groups and users who want to track the instrument history of their analytical systems The optional Agilent Maintenance Wizard Add on provides an easy to use step by step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Infinity II Series instruments The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files Agilent 1290 Infinity II High Speed Pump User Manual 45 5 46 Troubleshooting and Diagnostics Agilent Lab Advisor Software A
107. r Manual Hardware Information 10 RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for e 19200 baud 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 6 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Agilent 1290 Infinity lI High Speed Pump User Manual 173 10 Hardware Information 174 Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 A 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 ie 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 19 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down
108. resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG ERI remote cable see documentation for the APG ERI interface Agilent 1290 Infinity Il High Speed Pump User Manual 49 6 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input con
109. rmal phase 32 seals 33 0 operating Altitude 21 operating temperature 21 optimization achieving higher resolution 39 224 outlet valve replace 92 overcurrent of pump drive 65 overcurrent of solvent selection valve SSV 65 overview cable 154 P parts primary pump head 142 secondary pump head 144 PC and User Interface Software Setup 208 performance specifications 22 performance Optimization 35 physical specifications 21 power considerations 18 power consumption 21 power cords 19 pressure below lower limit 58 pressure exceeded upper pressure limit 57 pressure sensor calibration wrong or missing 73 pressure sensor replace 87 primary pump head assemble 118 disassemble 105 parts 142 product description 10 pump drive blocked or encoder failed 62 pump drive encoder defect 66 pump drive encoder error 71 pump drive encoder rollover 68 pump drive error 71 pump drive stop not found 72 pump drive stroke blocked 72 pump error messages 5 7 pump head assembly disassemble 103 reassemble 127 remove 101 pump head torques 89 purge valve failed 67 purge valve head replace 130 radio interference 219 reading of purge valve tag failed 67 reading the pump encoder tag failed 61 remote cables 158 remove doors 84 pump head assembly 101 repairs replacing firmware 135 replace gasket 116 high pressure filter assembly 132 inlet valve 90 jet weaver 95 outlet valve 92 pressure sensor 87 purge
110. s given on the screen 3 Close all shut off valves mie SS DIG in rates aie Agilent 1290 Infinity II High Speed Pump User Manual 101 7 Maintenance Pump Head Procedures 5 Remove the capillary connection at the top of the 6 Remove the flow connection between the degassing unit 7 Open the four screws holding the pump heads 8 Remove the complete pump head assembly by holding both heads and pulling it to the front NOTE Open the screws step by step not screw by screw 9 Proceed to install see Install the Pump Head Assembly on page 128 or disassemble the pump head see Disassemble the Pump Head Assembly on page 103 102 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance 7 Disassemble the Pump Head Assembly When If parts inside the pump head need to be replaced Tools required p n Description 5043 1361 Hex Key Set Driver 5023 2499 Hex Key Set 5023 2501 Screwdriver Torx T10 8710 1924 Wrench open 14 mm Preparations Remove the pump head I ey Damaged pump head Disassembling or reassembling the pump head with tools other than the ones recommended can damage pump heads and significantly reduce their life time gt Follow all instructions step by step gt Use recommended tools like the pump head alignment tool and a torque wrench 1 Place the pump head on a special holding tool 2 Open the screw at the top of the primary
111. sation in degasser chamber due to Equilibrate and restart module temperature fluctuation 2 Degasser is defect Please contact your Agilent service representative 58 Agilent 1290 Infinity II High Speed Pump User Manual Error Information 6 Degasser s pressure limit violation Error ID 22032 Pressure too far above the limit Probable cause Suggested actions 1 Leak in degasser chamber or degasser Please contact your Agilent service tubing representative 2 Defect vacuum pump Please contact your Agilent service representative 3 Degasser chamber empty or connected to Block unused degasser channels air Solvent counter exceeded limit Error ID 22055 The counter for the solvent volume has exceeded the limit which has been set in the user interface Parameter e Without Solvent Selection Valve 0 for channel A 1 for channel B e With Solvent Selection Valve 2 for channel Al 3 for channel B1 4 for channel A2 5 for channel B2 Probable cause Suggested actions 1 No solvent present Refill solvent bottle 2 Inappropriate setting Check solvent counter setting in user interface Agilent 1290 Infinity Il High Speed Pump User Manual 59 6 Error Information Waste counter limit exceeded Error ID 22056 The counter for the waste volume has exceeded the limit which has been set in the user interface e Parameter None Probable cause 1 The waste container is full 2 Inappropriate setting for waste counter
112. se Suggested actions 1 Blockage of system before pressure sensor Check for blockage of e g outlet valve filter frit purge valve heat exchanger 2 Drive motor defect Please contact your Agilent service representative Drive timeout Error ID 22410 Drive is blocked mechanically fails during initialization e Parameter 1 4 referring to pump drive Probable cause Suggested actions 1 Blockage of pump drive Drive motor defect Please contact your Agilent service representative Agilent 1290 Infinity II High Speed Pump User Manual Overcurrent of pump drive Error ID 22411 Error Information The current consumption of the pump drive is too high e Parameter 1 4 referring to pump drive Probable cause 1 Blockage of system before pressure sensor 2 Drive motor defect Suggested actions Check for blockage of e g outlet valve filter frit purge valve heat exchanger Please contact your Agilent service representative Overcurrent of solvent selection valve SSV Error ID 22412 Overcurrent of solvent selection valve SSV e Parameter None Probable cause 1 Valve defect Deliver underrun Error ID 22413 Internal error e Parameter None Probable cause 1 Internal error Agilent 1290 Infinity Il High Speed Pump User Manual Suggested actions Replace the solvent selection valve Suggested actions Please contact your Agilent service representative 65 6 6 Error
113. ser Manual Maintenance 7 Damage of the pump head assembly CAUTION When installing the pump head assembly the pump drives need to be in maintenance position where they are retracted Using the pump drive in default position will damage the pump head assembly gt Bring the pump drive to the maintenance position CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 1 Use the soft plastic side of the insert tool for inserting the 2 Insert the support ring and pump head ferrules into the piston seal to the pump chamber housing Wet the piston piston housing Observe the pins on the support ring seal with isopropanol or another suitable solvent before which help you assembling the pump head correctly insertion Insert tool Pump seal Pins second pin not shown Pump chamber housing Pump head ferrules Support ring Agilent 1290 Infinity II High Speed Pump User Manual 119 7 Maintenance Pump Head Procedures 3 Assemble the pump head by putting the pump chamber housing on top of the support ring Observe correct orientation of the pin Pump chamber housing Pump head ferrules Support ring Piston housing NOTE Do NOT install the inlet and outlet valves and the outlet filter at this stage 4 Loosely close the 4 s
114. signed automatically if your network supports Here aman on Laie a proves provides communication Altemate DNS s h coy Otherwise you need to ask your network administrator for IV Show icon in taskbar when connected automatic IP address via DHCP requires special IT setup of the network Figure 42 208 fas ae the appropriate IP settings Te Obtain an IP address automatically C Demeter scares C Obtain DNS server address automatically C Use the following DNS server addresses Changing the TCP IP settings of the PC Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 User Interface Software Setup Install you user interface software according the provided User Interface Software Setup Guide Agilent 1290 Infinity Il High Speed Pump User Manual 209 11 LAN Configuration PC and User Interface Software Setup Setup 210 Agilent 1290 Infinity II High Speed Pump User Manual Agilent 1290 Infinity Il High Speed Pump User Manual 12 Appendix General Safety Information 212 General Safety Information 212 Safety Standards 212 General 212 Before Applying Power 213 Ground the Instrument 213 Do Not Operate in an Explosive Atmosphere 214 Do Not Remove the Instrument Cover 214 Do Not Modify the Instrument 214 InCase of Damage 214 Solvents 215 Safety Symbols 216 Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 218 Radio Interference 219 Sound Emission 220 Agilent Tech
115. so allows to easily relocate the instrument to another Lab Condensation CAUTION Condensation within the module Condensation can damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 20 Agilent 1290 Infinity II High Speed Pump User Manual Physical Specifications Site Requirements and Specifications 2 Table 1 Physical Specifications Type Specification Comments Weight 21 0 kg 46 3 Ibs Dimensions 200 x 396 x 436 mm height x width x depth 7 9 x 15 6 x 17 2 inches Line voltage 100 240 V 10 Wide ranging capability Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC EN CSA UL 50 or 60 Hz 5 210 VA 180 W 4 55 C 39 131 F 40 70 C 40 158 F lt 95 r h at 40 C 104 F Non condensing Up to 3000 m 9842 ft Up to 4600 m 15092 ft For storing the module Installation category II Pollution degree2 For indoor use only Agilent 1290 Infinity Il High Speed Pump User Manual 21 2 Site Requirements and Specifications Performance Specifications 22 Table 2 Agilent 129
116. ss Enter to exit with storing parameters cx WINDOWS system32 cmd exe Agilent Technologies G4296A DEGHBHQH06 gt exit Connection to host lost CoN Figure 41 Closing the Telnet Session If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 134 40 27 99 Agilent 1290 Infinity Il High Speed Pump User Manual 207 11 LAN Configuration PC and User Interface Software Setup Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see also Initialization mode selection on page 185 FIXED IP address Local Area Connection Properties 2 x General General Connect using You can get IP setings automatically if your network supports this capability Ottierwise pape pig okronati a a Xircom CredtCard Ethemet 10 100 Modem 56 the appropriate IFiisettings Configure C DbteererrtPaddressautomatically Components checked are used by this connection Use the following IP address IP adiere 192 168 254 1 Subnet mask 255 255 248 0 Default gateway Install Uninstal h Pronertes c E Internet Protocol TCP IP Properties Description Use the folo General Transmission Control Protocol Intemet Protocol The default Prefered DNS You can get IP settings as
117. st the current settings SAC AC EE ee information about the LAN interface 7 MAC address initialization mode LAN Status Page Initialization mode is Using Stored active TCP IP settings TCP IP status here ready Init Mode Using Stored connected to PC with controller software e g Agilent ChemStation here not connected MAC Addre 6636D36AG838 TCP IP Properties active IP Address gt 134 40 27 95 Subnet Mask gt 255 255 248 0 Gateway 134 40 24 1 Controllers no connections Figure 39 Telnet Current settings in Using Stored mode 6 Change the IP address in this example 134 40 27 99 and type to list current settings AWINDOWS system32 cmd_exe telnet 134 40 27 95 STEER CLT change of IP setting to gt 4 Initialization mode is Using Stored LAN Status Page active TCP IP settings MAC Addre 838D38AG838 stored TCP IP settings in non volatile memory sing Stored TCP IP Properties active i IP Address 134 40 27 95 connected to PC with controller software e g Agilent Subnet Mask 255 255 248 0 ChemStation here not connected Def Gateway 134 409 24 1 stored IP Address gt 134 40 27 99 z 255 255 248 0 Gateway 134 40 24 1 Controllers z no connections Figure 40 Telnet Change IP settings 206 Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 7 When you have finished typing the configuration parameters type exit and pre
118. t should not exceed 2 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet All leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other Waste tubes are guided through a channel on the right hand side of the instrument keeping the front access clear from tubes The leak plane provides leak management by catching all internal liquid leaks guiding them to the leak sensor for leak detection and passing them on to the next module below if the leak sensor fails The leak sensor in the leak plane stops the running system as soon as the leak detection level is reached Solvent and condensate is guided through the waste channel into the waste container e from the detector s flow cell outlet from the Multisampler needle wash port e from the Sample Cooler condensate e from the Seal Wash Sensor e from the pump s Purge Valve or Multipurpose Valve The waste tube connected to the leak pan outlet on each of the bottom instruments guides the solvent to a suitable waste container Do not install the waste tubings into the central waste connectors Agilent 1290 Infinity II High Speed Pump User Manual Introduction 1 Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe c
119. ters i e LC1260 e IP address Comment optional e Subnet mask e Gateway address optional The configuration information entered is saved in the Tab File Click OK Leave Edit BootP Addresses by pressing Close Exit BootP Settings by pressing OK After each modification of the BootP settings G e EditBootPSettings a stop or start of the BootP service is required for the BootP service to accept changes See Stopping the Agilent BootP Service on page 201 or Restarting the Agilent BootP Service on page 202 oo N O 10 Power cycle the Instrument OR If you changed the IP address power cycle the instrument for the changes to take effect 11 Use the PING utility to verify connectivity by opening a command window and typing Ping 192 168 254 11 for example The Tab File is located at C Documents and Settings All Users Application Data Agilent BootP TabFile 200 Agilent 1290 Infinity II High Speed Pump User Manual LAN Configuration 11 Changing the IP Address of an Instrument Using the Agilent BootP Service Agilent BootP Service starts automatically when your PC reboots To change Agilent BootP Service settings you must stop the service make the changes and then restart the service Stopping the Agilent BootP Service 1 From the Windows control panel select Administrative Tools gt Services The Services screen appears RE Be aton yew teb s B iDRBI r gt r gt s n Select an tem to view ts descr
120. the Pressure Sensor 87 Overview of Torques for Pump Head Procedures 89 Replace the Inlet Valve 90 Replace the Outlet Valve 92 Replace the Solvent Selection Valve SSV 94 Change Configuration or Replace the Jet Weaver 95 Replace the Seal Wash Pump 96 Release a Stuck Inlet Valve 97 Pump Head Procedures 98 Replace the Purge Valve Head 130 Replace Parts of the High Pressure Filter Assembly 132 Install the Valve Rail Kit 134 Replace the Module Firmware 135 Prepare the Pump Module for Transport 136 Parts and Materials for Maintenance 137 Overview of Maintenance Parts 138 Flow Connections 139 Pump Head Assemblies 140 Pump Head Assembly Parts 141 Primary Pump Head Parts 142 Secondary Pump Head Parts 144 Purge Valve 146 Cover Parts 147 Leak Parts 147 Accessory Kit 148 Tools 149 HPLC System Tool Kit 151 Agilent 1290 Infinity II High Speed Pump User Manual Contents 9 Identifying Cables 153 Cable Overview 154 Analog Cables 156 Remote Cables 158 CAN LAN Cables 162 Agilent Module to PC 163 USB 164 10 Hardware Information 165 Firmware Description 166 Electrical Connections 169 Interfaces 171 Setting the 8 bit Configuration Switch 176 Early Maintenance Feedback 179 Instrument Layout 180 1 LAN Configuration 181 What You Have to Do First 182 TCP IP parameter configuration 183 Configuration Switch 184 Initialization mode selection 185 Dynamic Host Configuration Protocol DHCP 189 Link configurati
121. ting procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1290 Infinity II High Speed Pump User Manual In This Guide In This Guide This manual covers the Agilent 1290 Infinity II High Speed Pump G7120A 1 Introduction This chapter gives an introduction to the pump and an instrument overview 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Using the Pump This chapter explains the operational parameters of the Agilent 1290 Infinity II High Speed Pump 4 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 5 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 6 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 7 Maintenance This chapter describes the maintenance of the High Speed Pump Agilent 1290 Infinity lI High Speed Pump User Manual 3 In This Guide 8 Parts and Materials for Maintenance This chapter provides information on parts for maintenance 9 Identifying Cables This chapter pro
122. tings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 178 Agilent 1290 Infinity lI High Speed Pump User Manual 177 10 Hardware Information Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table9 Boot Resident Settings On board LAN Mode Select swi sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 1 0 0 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start
123. tion of the filter frit Hole Filter frit Seal cap 124 Agilent 1290 Infinity Il High Speed Pump User Manual Maintenance CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right torque consult the manual of your torque wrench 13 For the secondary pump head assemble and install the high pressure filter assembly using the torque wrench 14 mm hex wrench which is included to the 1290 Infinity Service Kit p n 5067 4699 set to 16 Nm 14 Remove the alignment piston Agilent 1290 Infinity II High Speed Pump User Manual 125 7 Maintenance 15 Lubricate the pump piston with isopropanol or another suitable solvent and insert it Piston 126 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Reassemble the Pump Head Assembly 1 Insert both pump heads to the link plate and make sure that the clips snap in that fix the pump heads Link plate Clips Observe the correct orientation of the primary and the secondary pump head This is important for correct fixation of the heat exchanger and the capillaries as described in the following steps Mind the orientation of the ID tag CAUTION Damage to the pump head Using a wrong torque will damage the pump head gt For handling the torque wrench setting and applying the right tor
124. tle e Flush all channels with water to remove salt deposits Visually inspect solvent filters Clean or exchange if necessary Power up Shut down the pump Power up the pump e Use new or different mobile phase as required e Purge pump heads with 2 5 3 mL min for 5 min Condition pump heads for 10 20 min Long term shut down of the pump Flush system with water to remove buffer Use recommended solvents to store the system e Power off the pump or system Agilent 1290 Infinity Il High Speed Pump User Manual 29 3 Using the Pump Prepare CAUTION the pump Purge Use the Purge function to e fill the pump exchange a solvent remove air bubbles in tubes and pump heads Condition Use the Conditioning function e daily when starting the pump to minimize pressure ripple by dissolving air bubbles in pump heads Condition your complete system with solvents and composition of your application for example 50 50 A B at 0 5 mL min Seal wash The seal wash function runs continuously and is controlled by the seal wash sensor This guarantees a maximum seal life time Contaminated seal wash solvent gt Do not recycle seal wash solvent to avoid contamination gt Weekly exchange seal wash solvent 30 Agilent 1290 Infinity II High Speed Pump User Manual Using the Pump 3 How to deal with solvents e Use clean bottles only e Exchange water based solvents daily Select solvent volume
125. to be used up within 1 2 days Use only HPLC grade solvents and water filtered through 0 2 um filters e Label bottles correctly with bottle content and filling date expiry date Use solvent inlet filters Reduce risk of algae growth use brown bottles for aqueous solvents avoid direct sunlight Agilent 1290 Infinity Il High Speed Pump User Manual 31 3 Using the Pump Normal Phase Applications CAUTION Current valves used with 1260 and 1290 Infinity pumps do not work well with applications using non polar solvents as for normal phase applications e g hexane and heptane With such applications pressure drops could be observed They are a result of particles electrostatically charging up in insulating solvents and sticking to the balls inside the valves such that the valves do not close properly any more after some time of use can be hours For normal phase applications a second type of valves is available which has a design based on the existing one for 1260 and 1290 Infinity valves These valves use a new material for valve balls which is a conductive ceramic and replaces non conductive ruby balls The balls do not charge up electrostatically and show good performance in normal phase The valves are marked with N for non polar or normal phase Agilent recommends using these valves for and only for normal phase applications Corrosion of valves Normal phase balls valves corrode quickly in aqueous solutions a
126. ts hardware MAC address as an identifier The Agilent BootP Service answers this request and passes a previously defined IP address and host name associated with the hardware MAC address to the requesting instrument The instrument receives its IP address and host name and maintains the IP address as long as it is powered on Powering down the instrument causes it to lose its IP address so the Agilent BootP Service must be running every time the instrument powers up If the Agilent BootP Service runs in the background the instrument will receive its IP address on power up The Agilent LAN Interface can be set to store the IP address and will not lose the IP address if power cycled Situation Cannot Establish LAN Communication If a LAN communication with BootP service cannot be established check the following on the PC e Is the BootP service started During installation of BootP the service is not started automatically Does the Firewall block the BootP service Add the BootP service as an exception e Is the LAN Interface using the BootP mode instead of Using Stored or Using Default modes Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 Installation of BootP Service Before installing and configuring the Agilent BootP Service be sure to have the IP addresses of the computer and instruments on hand 1 2 3 Log on as Administrator or other user with Administrator privileges Close all
127. uired p n Description 4 5067 4124 Shutoff valve 1 G7120 40004 Valve Holder Left 1 G4220 60035 Tubing kit 140 mm 2 pk SSV to shutoff valve or degassing unit Preparations In order to avoid leaks remove tubings from the solvent bottles 1 Unscrew tubing connections between shutoff valves solvent bottles and the solvent selection valve 2 If a single valve shall be replaced it can be pulled to the front for removing it from its mounting Remove the shutoff valve panel by pulling it downwards 4 After replacing the panel or after completion of other maintenance re install the panel and all flow connections 86 Agilent 1290 Infinity II High Speed Pump User Manual Maintenance 7 Replace the Pressure Sensor Replace the Pressure Sensor When No or invalid pressure signal Tools required p n Description 8710 2412 Hex key 2 5 mm 15 cm long straight handle 8710 0510 Wrench open 1 4 5 16 inch Screwdriver Parts required p n Description G7104 60001 Pressure sensor 1300 bar Preparations Turn off pump flow switch off pump NOTE This procedure describes how to replace the pressure sensor In case the cable to the sensor shall be replaced as well please contact your Agilent service representative 1 Remove capillary connections between the pressure 2 Remove the screws that fix the pressure sensor to the sensor and purge valve chassis Agilent 1290 Infinity II High Speed Pump Us
128. ves and Seals Table 3 Recommended valves and seals for normal phase applications 1260 Infinity 1290 Infinity Inlet valves 1260 Infinity Inlet Valve Type N 1290 Infinity Inlet Valve Type N G1312 60166 G4220 60122 1290 Infinity Quat Inlet Valve Type N G4204 60122 Outlet valves 1260 Infinity Outlet Valve Type 1290 Infinity Outlet Valve Type N SFC G1312 60167 N G4220 60128 Seals PE seals pack of 2 0905 1420 Wash Seal PE 0905 1718 Agilent 1290 Infinity Il High Speed Pump User Manual 33 3 Using the Pump Normal Phase Applications 34 Agilent 1290 Infinity II High Speed Pump User Manual Agilent 1290 Infinity II High Speed Pump User Manual 4 Optimizing Performance Delay Volume and Extra Column Volume 36 Delay Volume 36 How to Configure the Optimum Delay Volume 37 How to Achieve Higher Resolution 39 Using Solvent Calibration Tables 42 This chapter gives hints on how to optimize the performance or use additional devices Apg Agilent Technologies 35 4 Optimizing Performance Delay Volume and Extra Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Delay Volume In gradient separations this volume causes a delay between the mixture changing in the pump and that change reaching th
129. vides information on cables used with the modules 10 Hardware Information This chapter describes the pump in more detail on hardware and electronics 11 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC 12 Appendix This chapter provides addition information on safety legal and web Agilent 1290 Infinity Il High Speed Pump User Manual Contents Contents 1 Introduction 9 Product Description 10 Pump Principle 11 Leak and Waste Handling 13 2 Site Requirements and Specifications 17 Site Requirements 18 Physical Specifications 21 Performance Specifications 22 3 Usingthe Pump 25 Magnets 26 Turn on off 27 Status Indicators 28 Best Practices 29 Normal Phase Applications 32 4 Optimizing Performance 35 Delay Volume and Extra Column Volume 36 How to Configure the Optimum Delay Volume 37 How to Achieve Higher Resolution 39 Using Solvent Calibration Tables 42 5 Troubleshooting and Diagnostics 43 User Interfaces 44 Agilent Lab Advisor Software 45 6 Error Information 47 What Are Error Messages 49 General Error Messages 50 Pump Error Messages 57 Agilent 1290 Infinity lI High Speed Pump User Manual Contents 7 Maintenance 75 Introduction to Maintenance 77 Warnings and Cautions 79 Overview of Maintenance 81 Cleaning the Module 82 Install Fittings and Capillaries 83 Remove and Install Doors 84 Replace the Shutoff Valve Panel 86 Replace
130. ware and electronics ott Agilent Technologies 165 10 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections a non instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN USB and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN USB and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote error handling diagnostic functions e or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 166 Agilent 1290 Infinity Il High Speed Pump User Manual Hardware Information 10 Firmware Updates Firmware updates can be done using the following tools latest version should be used Agilent Lab Advisor software with files on the hard disk Firmware Update Tool with local files on the hard disk Instant Pilot G4208A with files on a
131. with step 1 Reproducible solvent properties are maintained by an integrated two channel solvent degasser It is located between solvent selection valve and the pump heads The pump automatically compensates for pressure and flow instabilities caused by the complex relationship between solvent compressibility and system pressure The only user interaction is selecting the appropriate solvent or solvent mixture per channel from a drop down list Agilent 1290 Infinity II High Speed Pump User Manual 11 1 12 Introduction Pump Principle Multisampler ds lala ih Seal wash pump Ssv m Pump head A E Seal wash sensor Jet Weaver Degassing unit High Speed Pump Figure2 The hydraulic path Agilent 1290 Infinity II High Speed Pump User Manual Introduction Leak and Waste Handling Leak and Waste Handling The 1290 Infinity II Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed i Ws W Y Vai X ESSN pe Ja F iit A 45h lt Fosi 1 Figure 3 Leak and waste handling concept overview typical stack configuration as an example Agilent 1290 Infinity II High Speed Pump User Manual 13 1 14 Introduction The solvent cabinet is designed to store a maximum volume of 8 L solvent The maximum volume for an individual bottle stored in the solvent cabine
132. ws that the optimum flow rate through an STM column is higher than for larger particles and is fairly flat as the flow rate increases Typical close to optimum flow rates for STM columns are 2 ml min for 4 6 mm i d and 0 4 ml min for 2 1 mm i d columns Agilent 1290 Infinity II High Speed Pump User Manual Optimizing Performance 4 In isocratic separations increasing the retention factor k results in better resolution because the solute is retained longer In gradient separations the retention is described by k in the following equation tc F100 A B V S m where k mean k value tg time length of gradient or segment of gradient min F flow ml min Vi column delay volume e A B change in fraction of solvent B during the gradient e S constant ca 4 5 for small molecules This shows that k and hence resolution can be increased by having a shallower gradient 2 to 5 min change is a guideline higher flow rate and a smaller volume column This equation also shows how to speed up an existing gradient if the flow is doubled but the gradient time is halved k remains constant and the separation looks the same but happens in half the time Recently published research has shown how a shorter STM column at temperatures above 40 C can generate higher peak capacity than a longer STM column by virtue of running it faster Refer to Petersson et al J Sep Sci 31 2346 2357 2008
133. y are still available when switching back to the mode Using Stored Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 Dynamic Host Configuration Protocol DHCP General Information DHCP The Dynamic Host Configuration Protocol DHCP is an auto configuration protocol used on IP networks The DHCP functionality is available on all Agilent HPLC modules with on board LAN Interface or LAN Interface Card and B firmware B 06 40 or above When the initialization mode DHCP is selected the card tries to download the parameters from a DHCP Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d3177321 It is the DHCP server s responsibility to forward the hostname address information to the Domain Name Server The card does not offer any services for hostname resolution e g NetBIOS DHCP Active Server Parameter Figure 28 DHCP Principle 1 It may take some time until the DHCP server has updated the DNS server with the hostname information 2 It may be necessary to fully qualify the hostname with the DNS suffix e g 0030d3177321 country company com 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conv
134. ysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 51 6 Error Information Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative
135. zation mode selection on page 185 Link Configuration speed and duplex mode determined by auto negotiation for details see Link configuration selection on page 192 184 Agilent 1290 Infinity Il High Speed Pump User Manual LAN Configuration 11 Initialization mode selection Table 12 The following initialization init modes are selectable Initialization Mode Switches SW6 SW7 SW8 Init Mode OFF OFF OFF Bootp OFF OFF ON Bootp amp Store OFF ON OFF Using Stored OFF ON ON Using Default ON OFF OFF DHCP 1 Requires firmware B 06 40 or above Modules without LAN on board see G1369C LAN Interface Card Bootp When the initialization mode Bootp is selected the module tries to download the parameters from a Bootp Server The parameters obtained become the active parameters immediately They are not stored to the non volatile memory of the module Therefore the parameters are lost with the next power cycle of the module Bootp gt Active Parameter Server Figure 24 Bootp Principle Agilent 1290 Infinity Il High Speed Pump User Manual 185 11 LAN Configuration 186 Bootp amp Store When Bootp amp Store is selected the parameters obtained from a Bootp Server become the active parameters immediately In addition they are stored to the non volatile memory of the module Thus after a power cycle they are still available This enables a kind of bootp once configuration of the module

Agilent 1290 Infinity II High Speed Pump User Manual

Contents

Download Pdf Manuals

Related Search

Related Contents