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Typhoon™ FLA 7000 Control Software

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1. 500 500 1000 c25 pm C L4 Filter F IP 80 pm oe c 100 um C 200 Sampling Area Mode Grid C Free AIBIC DIE FIG H I JIK LIM IN O P C 20x25 C 20x40 File Size about 7 63 MB x2 A BIC DIE FIGIH I JIKILIM IN O P Reading Time about 1m0s Top File Format Gel and TIFF O Start Scan ND Filter Off Click Top to return to the main window from the Phosphorimaging mode 2 Make these settings before reading Storage phosphor screens Explanations for the various settings are found below Function Image Folder Specify where the file is saved after reading by clicking the Browse button File Name Enter a file name to save data of a read image Note You cannot start reading unless you enter a file name C Comment Enter a comment optional The comment is saved with the image as a file and can be viewed with the analyzing software Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 9 2 Reading phosphorimaging samples 2 1 Setting the reading conditions Function Laser Filter Before reading begins make sure that the 650 nm laser is shown and the IP filter is selected in the Setting field as shown below In Phosphorimaging reading the 650 nm laser and IP filter are selected automatically Laser pa 650nm Filter F IF To use the Phosphorimaging mode the LD 650nm laser must be loaded and the IP filter must be set Ifthese conditions are not fulfilled th
2. File Format f Gel image File gel Gel Image File geh TIFF image Filet tif Launch Application w C 3 For File Format select Selection Function Gel Image File gel The standard file format is a gel file which contains square root encoded pixel data Gel Image File gel In combination with a gel file a read image can also be saved Tiff Image File tif in TIFF file format For TIFF files image data type is always set to Linear format 4 Click the Select button and select the specified analyzing software Launch Application C Program Files GE Healthcare lmageQuantTL 1d exe b elect 36 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 Installing and uninstalling the software 6 1 Installation Windows XP Installation is performed in the following sequence 1 Installation of USB Control driver 2 Installation of USB Function driver 3 Installation of Typhoon FLA 7000 Control Software 6 1 1 Installation of USB Control Driver Note The computer and Typhoon FLA 7000 must not be connected with a USB cable during the operation 1 Open the control panel and select Printers and Other Hardware amp Control Panel File Edit view Favorites Tools Help Q Back 7 S P Search Key Folders i gt Address gt Control Panel v Control Panel A Pick a category E Switch to Classic View G L A Appea
3. 4 3 Setting the Fluor stage on Typhoon FLA 7000 sss ssssesssssssssssssssssssssrsrssrrrrrrrerrren A N WSN aaa E E eee ees 5 Lasers and filters other settings cssccsscsssssssssssscessessseesssesssscssesssesseeesseeeses 5 1 Registering laser and filter COMbINnationsS ssssssssssssssssseeesserrrrrrrrrresesssssssssssssssseses 52 Filtermodule SEINS gene nnn nn eevee uni ne ve eevee Oe ee ee 521 Registering Mers enssins iere neve eee 5 2 2 Saving filter COMbINAtIONS scocsuvasisssiascessadecesuctatesivesuvesw vaseytedatesantadsenudediavestedendeteutact 52 3 Recalling Tilter cCOmMbOINGUONS ess tecacccenscartsan aac eiancnenaniian 5 2 4 Registering a new filter NAME sssrssssssssssssssssssresesrrrrresrrrrrsessssssssssssssssrrrrrrrrrrens 5 2 5 Edita filter name or remove a filter sssssssssssssssrrrrrerirrrrerrrrrssessssssssssssrrrrrrrrrren 5 3 Other settings earn nnn moO nn ne eee Sad SEUNG Or SCANNING seseina Ee ese tems cesses 5 3 2 Selecting file format and analyzing software u 6 Installing and uninstalling the Software eeseeseeseessessesseeserserseeseeseesseesesseeseeseeses 6 1 Installation Windows XP creer cca enter seearennctatean staan baenousenvenecucs 6 1 1 Installation of USB Control Driver vc cessescsssssecessesescessesessessssscsceessssecseseesecsesetensseenees 6 1 2 Installation OF USB Function driver sscesecccccessusssixsccustcernestaietiseeitetestossontnticehiecetoadennces 6 1 3 Installation of Typhoon FLA
4. e tis possible to scan using a different laser Failed to retrieve picture data from PC Check the PC setting Meaning e There was a failure in reading the image data from the computer e Check the computer environment Countermeasure e tis possible that the computer s processing ability has degraded Check the computer once when there are no devices connected to it and no software started PMT error occurred Use low sensitivity for the Setting Meaning e An overexposure error has been detected outside of the scanning area e Scan with low sensitivity settings Countermeasure e Scan with low sensitivity settings e Restart the computer e fan error occurs even after restarting the instrument call a serviceman The file name is already used Meaning e The file name is already used for another file Countermeasure e Change the file name to make sure that the scan data is not lost Close the SampleSetDoor or FilterChangeDoor Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA Meaning Close the sample set door or the filter change door Countermeasure Close the sample set door or the filter change door 69 7 Troubleshooting 7 2 Warnings Note If an error message is displayed a serviceman should take the countermeasures to resolve the trouble Please contact the dealer where you purchased Typhoon FLA 7000 or contact GE Healthcare For technical inquiry an
5. 000 635 1 TT File Laser cear Name Name Number If reading is set with the 635 nm laser 473 nm laser 635 nm laser and 635 nm laser with test as the file name then the following files are created in the test folder test 655 1 test 4 35 test 635 2 test 655 5 c Comment Enter a comment optional The comment is saved with the image as a file and can be viewed with the analyzing software d Scanning Method and PMT eta a B Scanning can be performed up to four times EE e7anmayscn Method and PMT are set for each scanning PHT S00 500 1000 Description Fe A From the pull down menu select the Method that corresponds EVER A with the sample The selected laser and filter combination is FITC displayed below Gy2 Dig ita 73 You may set the voltage to be applied to the photo multiplier tube PMT as an integral value within the predetermined range The larger the value is the higher the sensitivity PMT 500 W 500 1000 500V Value 1000V Small lt ___ Large Sensitivity Low lt P High 16 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 3 Reading fluorescent samples 3 1 Setting the reading conditions Description Click the button to increase the number of scans and click the button to reduce the number of scans Up to four scans can be performed e Pixel Size Set the pixel size for reading Click to select from one of the four types as shown
6. 16 6 1 16 3 1 33 1 1 66 4 Reading digitization samples 4 4 Starting reading Drag the adjuster You may adjust the density of the read image Data that are lighter than the line on the left Low value will be displayed as a completely white image and data that are darker than the line on the right High value will be displayed as a completely black image If Auto Range Scope is checked the Typhoon FLA 7000 Control Software automatically corrects the optimal tone You may change the display area by selecting a magnification ratio from the pull down menu In addition after reading is complete you can click the zoom in and zoom out button aia and then click in the display area to enlarge or reduce the magnification 4 Click Save as to save the data with a different file name Click Launch to launch the registered analyzing software to display the image 5 Toread another digitization sample continuously follow the above procedures Click the Return button to return to the first Reader Settings window Do not open the stage door of Typhoon FLA 7000 until the stage has completely returned If it is opened close it immediately 6 When scanning finishes the Save as and Launch buttons become active but the Return button is grayed out until the stage has completely returned 7 Finish reading Before turning off the power of Typhoon FLA 7000 shut down the Typhoon FLA 7000 Control Software Typhoo
7. 7000 The InstallShield R Wizard will install Typhoon FLA 7000 on your computer To continue click Mex WARMING This program is protected by copyright law and international treaties Cancel 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see back cover of this manual for contact information Select accept the terms in the license agreement and click the Next button 48 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 1 Installation Windows XP 6 1 3 Installation of Typhoon FLA 7000 Control Software ig Typhoon FLA 7000 InstallShield Wizard License Agreement Please read the Following license agreement carefully GE Healthcare STANDARD SOFTWARE END USER LICENSE AGREEMENT FOR LIFE SCANCES SOP TWAAE PRODUCTS IMPOR TANT PLEASE READ CAREFULLY THIS 15 THE LICENSE AGREEMENT THAT END USER I REQUIRED TO ACCEPT BEFORE INSTALLING AND USING GE HEALTHCARE SOFTWARE CAREFULLY READ ALL OF THE TERMS AND CONDITIONS OF THIS LICENSE AGREEMENT BEFORE PROCEEDING WITH THE DOWNLOADING AND OF a CT do not accept the terms in the license agreement 5 Select destination folder in the dialog ig Typhoon FLA 7000 InstallShield Wizard Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA Destination Folder Click Next bo install to this Folder or click Change to install to a different Fold
8. FT PROUT FEA PLL IPS Cat Weld We led ll Ready to Install the Program The wizard is ready to begin installation Click Install to begin the installation If you want to review or change any of your installation settings dick Back Click Cancel to exit the wizard InstallShield 64 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 3 Installation of Tyohoon FLA 7000 Control Software 7 Click the Finish button JE Typhoon FLA 7000 InstallShield Wizard InstallShield Wizard Completed The InstallShield Wizard has successfully installed Typhoon FLA 7000 Click Finish to exit the wizard Launch Typhoon FLA 7000 lt Back Cancel The installation of Typhoon FLA 7000 Control Software is now completed 8 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 65 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 3 Installation of Tyohoon FLA 7000 Control Software 6 4 Uninstallation Windows Vista 1 Select Uninstall a program underPrograms in Control Panel JON gt Control Panel e Control Panel Home Classic View Recent Tasks Uninstall a program Check for updates
9. PMT voltage adjustment mode differs from the regular Phosphorimaging mode in that a sensitivity level that matches the scanned sample can be set independently Replacement of the standard PMT with a multi alkali PMT is done by a serviceman For details contact an authorized dealer The Sensitivity settings differ from that of the regular Phosphorimaging mode The operation procedures for other settings are the same as those of the regular Phosphorimaging mode Follow the operation procedures of the regular Phosphorimaging mode Phosphorimaging mode Sensitivity Pixel Size Latitude C 10000 C 25 pm L4 m 54000 250 p a C 1000 C 100 pm C 200 pm PMT voltage adjustment mode PMT Pixel Size Latitude 500 V 500 1000 25 HM L4 50 pm E 100 pm 200 pm You may set the voltage to be applied to the PMT as an integral value within the predetermined range The larger the value is the higher the sensitivity but noise will be greater 500V Value 1000V Small lt gt Large Sensitivity Low lt High Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 3 Reading fluorescent samples 3 Reading fluorescent samples 3 1 Setting the reading conditions 1 Click the Fluorescence button The Reader Settings window for the Fluorescence mode is displayed lt amp Typhoon FLA 7000 Lee File Edit Help aq Condition File test1 dition Load Condition aS b Image Folder c Users UserAdmin Deskt
10. Select this to specify the Select this to specify a reading reading area arbitrarily area of 20 cm x 40 cm 53 Use Save Condition button when saving the reading conditions in a file You may save reading conditions that are used frequently with this function and recall them later with Load Condition Use Load Condition button when recalling reading conditions saved with Save Condition Note When starting Typhoon FLA 7000 Control Software the settings information from the previous session is displayed 2 2 Setting the Storage phosphor screen on the Phosphor stage Set the Storage phosphor screen on the Phosphor stage For instructions on setting the Storage phosphor screen see Typhoon FLA 7000 User Manual 2 3 Setting the Phosphor stage on Typhoon FLA 7000 Set the Phosphor stage on Typhoon FLA 7000 For instructions on setting the Phosphor stage on Typhoon FLA 7000 see Typhoon FLA 7000 User Manual Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 11 2 Reading phosphorimaging samples 2 4 Starting reading 2 4 Starting reading 1 Click the Start Scan button on the Reader Settings window to start reading Result The scanned area is displayed in the real time window as shown below The stage is read from the left to the right a Typhoon FLA 7000 Reader Scanning Condition Save File Manual Image Phosphor Low 0 High 65535 Curve Linear V Auto Range Scope Magnificat
11. exponential tone curve is used Linear to adjust gradations sigmoid Linear The linear tone curve is used to adjust gradations Sigmoid The sigmoid tone curve is used to adjust gradations 20 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 3 Reading fluorescent samples 3 4 Starting reading Drag the adjuster You may adjust the density of the read image Data of a lower light intensity than the line on the left Low value will be displayed as a completely white image and data of a higher light intensity than the line on the right High value will be displayed as a Low 20000 High 50000 completely black image w Auto Range Scope lf Auto Range Scope is checked the Typhoon FLA 7000 Control Software automatically optimizes the tone Note The Typhoon FLA 7000 Control Software converts data read from samples to images that have an information of 65536 tones with O being the value for white and 65535 being the value for black You may change the display area by selecting a Magnification 6 1 16 magnification ratio from the pull down menu In addition after reading is complete you can click the 3 1 83 zoom in and zoom out button and then click in the 1 1 66 display area to enlarge or reduce the magnification 4 Click Save as to save the data with a different file name Click Launch to launch the registered analyzing software to display the image 5 To read another fluorescent sam
12. on the left A sample with a smaller pixel size is analyzed more finely Short Reading Time Long lt lt ii Small Image File Size Large f Latitude Specify the dynamic range The dynamic range that can be detected is larger with L5 than with L4 If the signals of the sample are in the L4 range the density gradation is represented more finely if L4 is selected g Drag and select the scanning area on the Flour stage Sampling Area Mode Grid C Free C I CID EJ F File Size about 2 21 MB Reading Time about 1m0 s Function Grid mode Select Grid mode to specify the reading area based on the 2 5 cm grid lines on the Fluor stage Free mode Select Free mode to arbitrarily specify the reading area All mode Select All mode to specify the entire Fluor stage as the reading area Reading Time Displays estimated time until reading finishes File size Displays size of the file Expressed as file size per test x number of scans Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 17 3 Reading fluorescent samples 3 1 Setting the reading conditions When you use the Multi stage optional accessory When the gel with the glass and the titer plate sample are read by using the Multi stage and TP plugin change Stage type in the pull down list Display of Multi stage Sampling Area A IB CIDIE FIGIH I J K LIM N O P A BI CIDIE FIGIH 1 J KILIM N O P Drag and select the scanning area on
13. pour hardware amp If your hardware came with an installation CD it is recommended that you click Cancel to close this wizard and use the manufacturer s CD to install this hardware To continue click Hert Cancel Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 1 Installation Windows xP 6 1 1 Installation of USB Control Driver 4 Select Yes have already connected the hardware and click the Next button Add Hardware Wizard Is the hardware connected Have you already connected this hardware to your computer No have not added the hardware yet 5 Select Add a new hardware device and click the Next button Add Hardware Wizard The following hardware is already installed on your computer S From the list below select an installed hardware device then click Next to check properties or troubleshoot a problem you might be having To add hardware not shown in the list click Add a new hardware device Installed hardware e USE Root Hub oS USB Mass Storage Device amp Generic USB Hub ae USB Composite Device S Generic volume dd a new hardware device Cancel Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 39 6 Installing and uninstalling the software 6 1 Installation Windows XP 6 1 1 Installation of USB Control Driver 6 Select Install the hardware that I manually select from a list Advanced and click th
14. 7000 Control Software usses 6 2 Uninstallation For Windows XP sssssssssssonsssssssesssssssonsnsssononnnossrennssssnennssonrosnssssrennssnn 6 3 Installation Windows Vista wee ceecessecsesceccessescessessesecsessecsecsessessessesesseesecsecsucsecaesaeeneensees 6 3 1 Installation of USB control driver iiccicessteccres scccleccsssaessesztecieesscncaieezesiodionecaisea mes aeucen 6 3 2 Installation of USB function driver veeceesescssessesessssessessssssessssecseceessssecsecassessesatenseeeeees 6 3 3 Installation of Typhoon FLA 7000 Control Software sss 6 4 Uninstallation Windows Vista wc cecescessesssssescssesecsecseccecsescessessesesssesecsecsecsecatesecasensees Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA Table of Contents Table of Contents T TOUDE OCT ei arse erases ence ance sa sates cea errata cesses le ote ees 68 LE E och E E ttc Stebel etka ce ote cde e pee ded eee cts ateee nes eet 68 Le 0 6 a en SP TICE eT E eT ene ve ree 68 4 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 1 Preparations before use 1 Preparations before use 1 1 Starting Tyohoon FLA 7000 Control Software CAUTION Please note that the GUI screens may change without notice Note Minimum computer requirements OS Windows XP Professional SP3 32 bit or later or Windows Vista Business SP1 32 bit or later Memory More than 1 GB Processor Intel Core 2 Duo processors HD More than 80 GB
15. Change keyboards or other input methods 2 Select Typhoon FLA 7000 and then click Uninstall System and Maintenance Get started with Windows Back up your computer Security Check for updates Check this computer s security status ie Allow a program through Windows Firewall Network and Internet Connect to the Internet View network status and tasks Set up file sharing Hardware and Sound Play CDs or other media automatically Printer Mouse Programs Uninstall a program Change startup programs User Accounts Add or remove user accounts Appearance and Personalization Change desktop background Change the color scheme Adjust screen resolution Clock Language and Region Change keyboards or other input methods Ease of Access Let Windows suggest settings Optimize visual display Z Additional Options x gG A gt Control Panel Programs Programs and Features v Tasks View installed updates Get new programs online at Windows Marketplace View purchased software digital locker Turn Windows features on or off 66 Uninstall or change a program To uninstall a program select it from the list and then click Uninstall Change or Repair Organize v 22 Views Uninstall s Change Repair Name A E DeCyder 2D Database DeCyder 2D Oracle Server i DeCyder 2D Program dy ImageMaster 2D Platinum 7 ImageQuantTL E Intel R Graphics Media Accelerator
16. Control Software User Manual 28 9607 63 AA 25 4 Reading digitization samples 4 4 Starting reading 2 Click the Start Scan button to start reading Typhoon FLA 7000 Reader Scanning Condition Save File Coomassie Digitization Silver Stain Magnification 10 1 10 A Q Status Post reading Method Silver Stain Laser J 473nm Filter 520 File Format Gel and TIFF Low 0 High 65535 Curve Linear z V Auto Range Scope Return The scanned area is displayed in the real time window as shown below The stage is read from the left to the right 3 When you want to change the display parameters of the real time window refer to the explanations below and make settings The Typhoon FLA 7000 Control Software converts data read from samples to images that have an information of 65536 tones with 0 being the value for white and 65535 being the value for black The tones are indicated by the horizontal axis of the tone curve graph Curve Exponential 7 Auto kaaa TP Exponential sigmoid Linear Sigmoid 26 Select the type of tone curve from the pull down menu The exponential tone curve is used to adjust gradations The linear tone curve is used to adjust gradations The sigmoid tone curve is used to adjust gradations Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA Low 20000 High 50000 wW Auto Range Scope Magnification 6 1
17. Driver E9 Intel R Network Connections 13 1 33 1 _ Intel R TV Wizard _ IQTLSecurlTy fa Microsoft NET Framework 1 1 8 Microsoft NET Framework 3 5 SP1 Gi seeeesseeesnsneeeenesessnssseneenenenesseses Publisher GE Healthcare GE Healthcare GE Healthcare GE Healthcare GE Healthcare Intel Corporation Intel Intel Corporation GE Healthcare Microsoft Microsoft Corporation seueeesueususneenennssssssenseeeenesesessssssenenensensns Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 4 Uninstallation Windows Vista 3 Click Yes to uninstall the program 4 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 67 7 Troubleshooting 7 Troubleshooting 7 1 Errors An error is the condition in which all of the reading modes of Typhoon FLA 7000 cannot be used When an error occurs the indicator lamps light up and there is a beeping sound An error message dialog box is displayed on the Typhoon FLA 7000 Control Software screen displayed on the computer x Scanner error Please restart Typhoon FLA 7000 imager and Scanner Control Software Sense Key 04H Error Code EO40H 00000001 H Please contact GE Healthcare and be prepared to state the four digit Error Code and the eight digit num
18. GE Healthcare Typhoon FLA 7000 Control Software User Manual Table of Contents 1 Preparations before uSe esseeseeseeseessesseseeeserserseescescessessesseescesceseeesessesseeseeseeseeesess 1 1 Starting Typhoon FLA 7000 Control Software ssss ssssssssessssssssssssssesersrrrrrrrerrrreen 1 2 Control Software main WINdOW sssssssssssssssssssesssesttetteeeerrreeeeeesssssssssssssssssttttrirerrerereress 2 Reading phosphorimaging samples ccccssccsssssssecstecsseesscsssessseesseeseeesseeseees 2 1 Setting the reading Conditions ss iisssssiiiisissiniiirsrsrsisisiisssssdiainssssininrorissisisiisissssn 2 2 Setting the Storage phosphor screen on the Phosphor stage 2 3 Setting the Phosphor stage on Typhoon FLA 7000 sssssssssssssssseereereserrresssssssssssss eA SAn N ee chet aces tee ee eee scenes ttt cee 3 Reading fluorescent samples iicciiknsasiesinanuniendnws wanna Dok Setting the reading conditions sassssssnsceriniraseredsiniiuuni inah 3 2 Setting a fluorescent sample on the Fluor or Multi Stage usss 3 3 Setting the Fluor or Multi stage on Typhoon FLA 7000 sss 3 4 Starting redding Reeeanee nono nn nan nee nS EO On 4 Reading digitization samples cssccsscsscssscscestecssecssessescssecsseesscssseesseessseeseeees 4 1 Setting the reading Conditions s sssssssssssssssseessssirrrerrerrrssesssssssssssssssessesttrrrirrrerrerrerees 42 Setting a digitization sample on the Fluor Stage s src
19. MB 51 6 Installing and uninstalling the software 6 2 Uninstallation For Windows XP 3 Click Yes Add or Remove Programs 2 Are yau sure you want to remove Typhoon FLA 7000 From your computer Result A progress bar is displayed and uninstallation is started Note Correction files created during calibration such as shading data are required by the Typhoon FLA 7000 Control Software They are not deleted during the uninstallation and remain in the Data folder of Typhoon FLA 7000 Control Software folder 6 3 Installation Windows Vista Installation is performed in the following sequence 1 Installation of USB Control driver 2 Installation of USB Function driver 3 Installation of Typhoon FLA 7000 Control Software 6 3 1 Installation of USB control driver Note The computer and Typhoon FLA 7000 must not be connected with a USB cable during the operation Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation 52 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 1 Installation of USB control driver 1 Open the control panel and click Classic View in the upper left corner gO b Control Panel gt Control Panel Home Recent Tasks Un
20. Tell me why diver signing is important 9 Insert the Typhoon FLA 7000 Control Software CD and click the Browse button Install From Disk Insert the manufacturer s installation disk and then make sure that the corect dive is selected below a Copy manufacturer s files from m Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 41 6 Installing and uninstalling the software 6 1 Installation Windows XP 6 1 1 Installation of USB Control Driver 10 Select to install the driver from the Tyohoon FLA 7000 Control Software CD Locate File My Documents H My Computer See Local Disk LC s Typhoon FLA 7000 C Sam STORE N GO E O Shared Documents 9 My Documents A My Network Places File name DeyvMng int a Open Files of type Setup Information inf Cancel 11 Open the USB Control folder Locate File ok T USE Control CUSE Function File name Devhing inf k L Files of type Setup Information inf Cancel 42 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 1 Installation Windows xP 6 1 1 Installation of USB Control Driver 12 Select the DevMng inf file and click the Open button Locate File Look in O USB Control i ee E 4 Files of type Setup Information inf l 13 Click the OK button Install From Disk Insert the manufactur
21. USB ports USB 2 0 Optical drives DVD ROM or SuperMulti Drive Monitor more than 1280 x 1024 resolution NOTICE Do not connect any USB devices other than Typhoon FLA 7000 to the computer in which the Typhoon FLA 7000 Control Software is installed Otherwise it may cause malfunctions During reading do not use any USB devices other than Typhoon FLA 7000 connected to the computer If USB devices are used simultaneously image data may be lost 1 Turn on Typhoon FLA 7000 and peripheral devices CAUTION A N Do not insert a Storage phosphor screen in Typhoon FLA 7000 before turning on the machine If an imaging plate is detected during the self diagnosis of the Typhoon FLA 7000 the sensitivity of the Storage phosphor screen may deteriorate The scanned data can then not be guaranteed USB Connection Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 5 1 Preparations before use 1 1 Starting Tyohoon FLA 7000 Control Software 2 Turnon the computer 3 Make sure that Typhoon FLA 7000 has completed the warm up Only the power lamp on the upper left panel on the front of Typhoon FLA 7000 is lit up when the warm up is completed 4 Start Typhoon FLA 7000 Control Software from the Startup menu or use the shortcut key Typhoon FLA Yood Note After starting the Control Software the condition is displayed in the Status area Status messages are as follows e Disconnected Cannot
22. ber inside the parentheses 7 2 Warnings 68 The following are examples of warnings that are displayed as messages in the Typhoon FLA 7000 Control Software window If a displayed message includes instructions please follow them Message Meaning and Countermeasure Filter module has not been Meaning Setup e A filter module has not been set Push the filter module button and set up the filter module Countermeasure e Click the filter module button and set a filter module e Confirm that a filter module is set e If not set a filter module using the filter module button The combination of the Meaning lasers and filters could be e There is a possibility that the laser and filter combination is Inappropriate inappropriate Please check the laser and filter Check the lasers and filters E E e Confirm the Methods registered It is possible that the laser and filter combination is inappropriate e Confirm that the filter specified in the Typhoon FLA 7000 Control Software is actually set Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA Message 7 Troubleshooting 7 2 Warnings Meaning and Countermeasure Laser error occurred Use other lasers Meaning e A laser error has occurred e Usea different laser Countermeasure e An error has occurred with the laser Restart the instrument e ifan error occurs even after restarting the instrument call a serviceman
23. cent Places D DVD RW Drive D Typhoon FLA 7000 test a ORE N GO E Ay Setup Information inf 56 7 Insert the Typhoon FLA 7000 Control Software CD and click the Browse button OK te modif bacain Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 9 Open the USB Control folder LY Computer Aw c Network E DVD RW Drive D Typhoon FLA 7000 Name Size Type Date modif Location Files Currently on the Disc 2 USB Control a File Folder USB Function File Folder File name int Files of type Setup Information inf Deng OO O O O OO O O O l Setup Information inf Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 3 Installation Windows Vista 6 3 1 Installation of USB control driver 57 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 1 Installation of USB control driver 11 Click the OK button Insert the manufacturer s installation disk and then make sure that the drive is selected below 12 Click the Next button Select the device driver you want to install for this hardware Select the manufacturer and model of your hardware device and then click Next If you have a disk that contains the driver you want to install click Have Disk Model Fujifilm USB Device Managment Driver ity This driver is not digitally signed Tell me driver signing is impo
24. continue cick Next WARNING This program is protected by copyright law and international treaties 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative See the back cover of this manual for contact information Select accept the terms in the license agreement and click the Next button JE Typhoon FLA 7000 InstallShield Wizard Please read the following license agreement carefully GE Healthcare STANDARD SOFTWARE END USER LICENSE AGH FOR LIFE SCIENCES SOFTWARE PRODUCTS IMPORTANT PLEASE READ CAREFULLY THIS IS THE LICENSE AGREEMENT THAT END USER IS REQUIRED TO ACCEPT BEFORE INSTALLING AND USING GE HEALTHCARE SOFTWARE CAREFULLY READ ALL OF THE TERMS AND CONDITIONS OF THIS LICENSE AGREEMENT BEFORE PROCEEDING WITH THE DOWNLOADING AND OR x I accept the terms in the license agreement 0 I do not accept the terms in the license agreement InstallShield Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 63 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 3 Installation of Tyohoon FLA 7000 Control Software 5 Select destination folder in the dialog Install Typhoon FLA 7000 to Ba Installshield e Click the Next button to install the software at the default folder C Program Files e Click the Change button to install to a different folder 6 Click the Install button Se ee ee ee T EL I Lad
25. d any question on application related matters price of the products etc please contact us to the address on the back cover 70 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com quantitative_imaging imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Typhoon is a trademark of GE Healthcare companies All third party trademarks are the property of their respective owners 2009 General Electric Company All rights reserved First published Oct 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9607 63 AA 09 2009
26. data read from samples to images that have an information of 65536 tones with O being the value for white and 65535 being the value for black Magnification 10 1 10 E You may change the display area by selecting a magnification ratio from the pull down menu In addition 10 1 10 rahe 5 1 20 after reading is complete you can click the zoom in and 2 4 40 zoom out button and then click in the display area to enlarge or reduce the magnification 3 Click Save as to save data with a different file name Click Launch to display the image using the registered analyzing software 4 To read a second Storage phosphor screen continuously follow the above procedures Click the Return button to return to the first Reader Settings window Do not open the stage door of Typhoon FLA 7000 until the stage has completely returned If it is opened close it immediately When scanning finishes the Save as and Launch buttons become active but the Return button is grayed out until the stage has completely returned 5 Finish reading Before turning off the power of Typhoon FLA 7000 shut down the Control Software Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 13 2 Reading phosphorimaging samples 2 4 Starting reading Note If the photo multipliers PMT are replaced with a multi alkali PMT the Phosphorimaging mode switches from that of the regular Phosphorimaging mode to a PMT voltage adjustment mode The
27. e 6 3 Installation Windows Vista 6 3 1 Installation of USB control driver 5 Select Show All Devices and click the Next button Add Hardware From the list below select the type of hardware you are installing IF you do not see the hardware category you want click Show All Devices Common hardware types Show All Devi _ Display adapters Cg IDE 4T4Z44TAPI controllers EEE 1284 4 compatible printer EEE 1284 4 devices m IEEE 1394 Bus host controllers z Imaging devices F Infrared devices E Wedis Cantor Pvtender 6 Click the Have Disk button Add Hardware Select the device diver you want to install for this hardware Select the manufacturer and model of your hardware device and then click Next IF ou ii have a disk that contains the diver you want to install click Have Disk Manufacturer Model Standard Infrared Port Standard Modern Types Standard M7 P Compliant Dey 4 Tl I Tell me why diver signing is important z Back Hegt gt Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 55 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 1 Installation of USB control driver Insert the manutacturer s installation disk and then make sure that the corect drive is selected below Copy manufacturer s files from Look in DVD RW Drive D Typhoon FLA 7000 7 Ea Recent tems My Desktop Network Publi JEE Computer Local Disk C Re
28. e edited Click the Delete button to delete a registered filter Default filters cannot be deleted Corresponds to the numbers 4 3 2 1 on the filter tray List of filters currently registered in the software Filters marked with are the filters that are initially set as default Click the Load Filter Module button to load saved filter combinations 2 Exchange the filter of Tyohoon FLA 7000 For instructions on exchanging the filter see Typhoon FLA 7000 User Manual 30 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 5 Lasers and filters other settings 3 Click filter position No 2 A red frame appears around the selected filter position 4 Select Y520 from the Filter List The selected item is highlighted in blue f Filter Module Settings Filter Module Settings i Filter List 0580 4 R670 3 520 lt 2 Filter Module Module1 1 B Py 2 Y520 ue P i Load Filter Module 4 B 0580 Delete Add Save Filter Module Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA Cancel OK 5 2 Filter module settings 5 2 1 Registering filters 31 5 Lasers and filters other settings 5 2 Filter module settings 5 2 1 Registering filters 5 Click the Insert button Filter Module Settings Filter Module Settings Filter List gt oso 4 R670 3 f 520 lt 2 IP lt Remove Filter Module Modu
29. e Next button Add Hardware Wizard The wizard can help you install other hardware a The wizard can search for other hardware and automatically install it for wou Or if you know exact which hardware model you want to install you can select it from a list What do wou want the wizard to do Search for and install the hardware automatically Recommended 7 Select Show All Devices and click the Next button Add Hardware Wizard From the list below select the type of hardware you are installing If you do not see the hardware category vou want click Show All Devices Common hardware types Show All Devi a Display adapters amp Fujifilm USB Device 4 IDE ATAZATAPI controllers Sef IEEE 1394 Bus host controllers 38 maging devices al Infrared devices Intel AIM Codec B hindame 40 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 1 Installation Windows xP 6 1 1 Installation of USB Control Driver 8 Click the Have Disk button Add Hardware Wizard Select the device driver you want to install for this hardware a Select the manufacturer and model of Your hardware device and then click Nest IF you have a disk that contains the driver you want to install click Have Disk Manufacturer Standard Infrared Port Standard Modem Types Standard port types Standard system devices This driver ig digitally signed Have Disk i
30. e Reader Settings window for the Phosphorimaging mode cannot be accessed PMT You may set the voltage to be applied to the photo multiplier tube PMT as an integral value within the predetermined range The larger the value is the higher the sensitivity 500V Value Small PMT 500 W 500 1000 ww gt Pixel Size Set the pixel size for reading Click to select from one of the four types as shown on the left A sample with a smaller pixel size is analyzed more finely 200 100 50 m25 Short Reading Time Long e S Small Image File Size Large Latitude Specify the dynamic range The dynamic range that can be detected is larger with L5 than with L4 If the signals of the sample are in the L4 range the density gradation is represented more finely if L4 is selected Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 2 Reading phosphorimaging samples 2 1 Setting the reading conditions Function Sampling Area Drag and select the scanning area on the Phosphor stage Sampling Area Mode Grid C Free A IBIC DIE FIGIH4I J IKIL IMIN O P C 20x25 C 20x 40 File Size about 312 50 KB A BIC DIE FIGIH I J K LIM IN O P Reading Time about 0 m 30 s The different modes on the Sampling Area are selected as follows Function Function Select this to specify the Select this to specify a reading reading area based on the 2 5 area of 20 cm x 25 cm cm grid lines on the Phosphor stage
31. eeds your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Connect the computer and the Typhoon FLA 7000 with a USB cable and turn ON the power switch of Typhoon FLA 7000 Result The scanner will automatically be detected by the computer and the Plug and Play function in Windows Vista starts 60 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 2 Installation of USB function driver 2 Inthe Found New Hardware dialog select Locate and install driver software recommended Windows needs to install driver software for your FUJIFILM FLA 7000 Locate and install driver software recommended Windows will guide you through the process of installing driver software for your device gt Ask me again later Windows will ask again the next time you plug in your device or log on Don t show this message again for this device Your device will not function until you install drrver software 3 Insert the Typhoon FLA 7000 Control Software CD and click the Next button in the Found New Hardware dialog Insert the disc that came with your FUJIFILM FLA 7000 If you have the disc that came with your device insert it now Windows will automatically search the disc for driver software Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 61 6 Installing and uni
32. eferences Click when reading a Storage phosphor Click to set the display menu file format or screen the Log Square Root of image data type 2 Fluorescence 7 Filter Click when reading fluorescent samples The loaded filters are displayed 5 Digitization 8 Laser Click when digitizing The conditions of the loaded laser units are displayed In this example there are four types of laser units loaded e 532 nm laser e 473 nm laser e 650 nm laser e 635 nm laser All the laser units are ready for operation OK Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 1 Preparations before use 1 2 Control Software main window Method Status Ready Click to register or erase combinations of The status of the FLA 7000 is displayed When lasers and filters the status is Ready The FLA 7000 is ready for scanning Filter Module Click to change or register the filter 8 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 2 Reading phosphorimaging samples 2 Reading phosphorimaging samples 2 1 Setting the reading conditions 1 Click the Phosphorimaging button The Reader Settings window for the Phosphorimaging mode is displayed Typhoon FLA 7000 File Edit Help Condition File _Save Condition Load Cond ition q E Image Folder Browse p Comment G Reader Settings Phosphorimaging d Laser J 650nm PMT Pixel Size Latitude i ilter
33. er Install Typhoon FLA 7000 ta C Program Files Cancel Click the Next button to install the software at the default folder C Program Files Click the Change button to install to a different folder 49 6 Installing and uninstalling the software 6 1 Installation Windows xP 6 1 3 Installation of Typhoon FLA 7000 Control Software 6 Click the Install button ig Typhoon FLA 7000 InstallShield Wizard Ready to Install the Program The wizard is ready to begin installation Click Install to begin the installation TF you want to review or change any of your installation settings click Back Click Cancel to exit the wizard Installshield 7 Click the Finish button ig Typhoon FLA 7000 InstallShield Wizard InstallShield Wizard Completed The InstallShield Wizard has successfully installed Typhoon FLA FOOO Click Finish to exit the wizard Launch Typhoon FLA 7000 Cancel The installation of Typhoon FLA 7000 Control Software is now completed 50 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 2 Uninstallation For Windows XP 1 Open the control panel and select Add or Remove Programs amp Control Panel File Edit View Favorites Tools Help va Control Panel Pick rs category B Switch to Classic view See Also ne Q a ke Appearance and Themes e W Windows Update i Help and Support s Network and Internet Connections User Accounts 6 Other Control Pa
34. er s installation disk and then ee make sure that the corect dive is selected below Cancel Copy manufacturer s files from D SUSB Control M Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 43 6 Installing and uninstalling the software 6 1 Installation Windows XP 6 1 1 Installation of USB Control Driver 14 Click the Next button Add Hardware Wizard Select the device driver you want to install for this hardware a Select the manufacturer and model of Your hardware device and then click Nest IF you have a disk that contains the driver you want to install click Have Disk Model Fujifilm USE Device Managment Driver ity This driver is not digitally signed Tell me why driver signing is inportant 15 Click the Next button Add Hardware Wizard The wizard ts ready to install your hardware Hardware to install Fujifilm USB Device Managment Driver To start installing your new hardware click Hert Cancel 44 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 1 Installation Windows XP 6 1 1 Installation of USB Control Driver 16 Click the Continue Anyway button Hardware Installation The software you are installing tor this hardware Fujifilm USE Device Managment Driver has not passed Windows Loga testing to very its compatibility with Windows P Tellme why this testing is important Continuing your insta
35. filter combinations 2 Click the Add button The following dialog box appears Name Enter a name for the combination to be N ame GET registered Laser 670nm Filter f Y520 Laser Select the type of laser You may also select ox ane fi i lasers that are not actually loaded Filter Select the type of filter You may also select lasers that are not actually loaded Note Combinations of lasers and filters that are not loaded cannot be selected in the Reader Condition screen 3 Enter a name for the combination select the type of laser and filter and click the OK button The laser and filter combination is registered Note Click the Delete and Edit buttons to delete or change a registered Method Methods initially registered as default cannot be deleted or edited The Methods marked with are the Methods that initially were set as default 4 Click the OK button 5 2 Filter module settings 5 2 1 Registering filters Filter module settings are registered by a serviceman upon installation Under normal circumstances it is not necessary to register these settings After exchanging the filters of Typhoon FLA 7000 you must register the exchanged filters in the Control Software Note Newly installed filters must be registered in the Control Software or they will not be displayed in the Control Software window The following explains the method for registering filters in the Control Softwa
36. he filter combination you want to recall and click the OK button The filter combination is recalled 4 Click the OK button Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 33 5 Lasers and filters other settings 5 2 Filter module settings 5 2 4 Registering a new filter name 5 2 4 Registering a new filter name You may register a filter name 1 Click the Filter Module button on the main window 2 Click the Add button The following dialog box appears Name Enter a name for the filter Icon Select the color of the filter icon to be displayed in the software 3 Select the filter name and icon color you want to register and click the OK button The filter is registered 4 Click the OK button 5 2 5 Edit a filter name or remove a filter Registered filter name can be deleted using Delete while Remove is used to empty a filter position in the filter module in the software To delete or edit the register filter name select the filter name and click the Delete or Edit button Methods initially registered as default cannot be deleted or edited Click the Remove button to keep the filter position empty after removing a filter 5 3 Other settings These are settings that are carried out when specifying settings for reading samples Depending on the reading mode there may be some functions that cannot be used 5 3 1 Settings for scanning 1 Click the Preferences button on the main window 34 Typ
37. hoon FLA 7000 Control Software User Manual 28 9607 63 AA 5 Lasers and filters other settings 5 3 Other settings 5 3 1 Settings for scanning 2 Click the Scan Settings tab Image File Settings For all modes Correction Mode tf Auto f Manual For fluorescence mode Scan Mode f Standard f Quick 5 For For fluorescence mode ND Filter select Selection Function Off Does not use ND filter for adjusting light intensity On Uses ND filter for adjusting light intensity 4 For For fluorescence mode Scan Mode select Standard or Quick If Quick is selected reading time will become shorter However the noise would stand out during reading The reading time varies depending on the setting image size and the scanning area When reading the whole area of the Fluor stage the reading times are as follows Standard Mode 200 um 210 s 100 um 210 s 50 um 330 s 25 um 450 s Quick Mode 200 um 150 s 100 um 150 s 50 um 210 s 25 um 330 s 5 Click the OK button 5 3 2 Selecting file format and analyzing software These are settings for saving images Depending on the scanning mode there may be some functions that cannot be used 1 Click the Preferences button on the main window Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 35 5 Lasers and filters other settings 5 3 Other settings 5 3 2 Selecting file format and analyzing software 2 Click the Image File Settings tab Preferences
38. igitization sample on the Fluor stage After setting the digitization sample on the Fluor stage place the fluorescent plate for digitization on top of it For instructions on setting the digitization sample see Typhoon FLA 7000 User Manual 24 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 4 Reading digitization samples 4 3 Setting the Fluor stage on Typhoon FLA 7000 4 3 Setting the Fluor stage on Typhoon FLA 7000 Set the Fluor stage on Typhoon FLA 7000 For instructions on setting the Fluor stage on Typhoon FLA 7000 see Typhoon FLA 7000 User Manual 4 4 Starting reading 1 Click the Start Scan button on the Reader Settings window to start reading Result The scanned area is displayed in the real time window as shown below The stage is read from the left to the right Typhoon FLA 7000 Reader Scanning Condition Save File Coomassie Digitization Silver Stain Low 0 High 65535 Curve Linear X V Auto Range Scope Magnification 10 1 10 A Q Status Post reading Method Silver Stain Laser J 473nm Filter 520 Return File Format Gel and TIFF Note To stop reading before the scanning is completed click the Stop button e The area that has not been read will be saved as an image with a data value of 0 light intensity of O e The reading function is cancelled You cannot start reading again from the location where reading stopped Typhoon FLA 7000
39. install a program Check for updates Change keyboards or other input methods 2 Open Add Hardware GO b Control Panel System and Maintenance Get started with Windows Back up your computer Security Check for updates Check this computer s security status e Allow a program through Windows Firewall _ Network and Internet Connect to the Internet View network status and tasks Set up file sharing Hardware and Sound Play CDs or other media automatically Printer Mouse Programs Uninstall a program Change startup programs 4 User Accounts Add or remove user accounts Appearance and Personalization Change desktop background Change the color scheme Adjust screen resolution Clock Language and Region Change keyboards or other input methods Ease of Access Let Windows suggest settings Optimize visual display Additional Options Control Panel Home e Classic View People Near Performance Personaliz Me a amp Programs Regionaland Scannersand Security and Features Language Zz Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA AutoPlay I fa Folder C iSCSI Initiator Backup and Restore C Management Controllers and Offline Files Sharing Ce Cider Ea P amp Mouse Date and Time D Indexing Options re Pen and Input Devices Intel R GMA Driver 3 Pvy Informatio Cameras A Phone and Mode
40. ion 14 1 7 Q Q Stop Status Reading 19 9 Method Phosphor Laser JJ 650nm Filter IP File Format Gel and TIFF Note To stop reading before the scanning is completed click the Stop button e The area that has not been read will be saved as an image with a data value of 0 light intensity of O e The reading function is cancelled You cannot start reading again from the location where reading stopped 2 When you want to change the display parameters of the real time window refer to the explanations below Curve Exponential Select the type of tone curve from the pull down menu w Auto T Exponential The exponential tone curve is used to E adjust gradations Sigmoid Linear The linear tone curve is used to adjust gradations Sigmoid The sigmoid tone curve is used to adjust gradations 12 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 2 Reading phosphorimaging samples 2 4 Starting reading Drag the adjuster You may adjust the density of the read image Data of a lower light intensity than the line on the left Low value will be displayed as a completely white image and data of a higher light intensity than the line on the right High value will be displayed as a completely black image Low 20000 High 50000 Auto Range Scope lf Auto Range Scope is checked the Control Software automatically optimizes the tone Note The Control Software converts
41. le 1 E IP 2 520 3 R670 Load Filter Module 4 ij 0580 Save Filter Module Delete Add Cancel OK It is also possible to drag and drop the selected filter to filter position No 2 Filter position No 2 changes to Y520 6 Click the OK button to save the selection 5 2 2 Saving filter combinations You may save the filter combination that is currently displayed Note Exchanging the module and saving recalling filter combinations can be managed more easily if each user has their own filter module 1 Click the Filter Module button on the main window 2 Click the Save Filter Module button The following dialog box appears 32 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 5 Lasers and filters other settings 5 2 Filter module settings 5 2 2 Saving filter combinations Filter Module Name Enter a name for the filter combination Save Filter Module Filter Module Name Module1 om cane 3 After entering a name for the filter combination click the OK button The filter combination is saved 4 Click the OK button 5 2 3 Recalling filter combinations You may recall the filter combinations that are currently registered 1 Click the Filter Module button on the main window 2 Click the Load Filter Module button The Load Filter Module dialog box is displayed Load Filter Module Filter Module List Delete OK 3 Select the name of t
42. llation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing STOP Installation 17 Click the Finish button Add Hardware Wizard Completing the Add Hardware Wizard The following hardware was installed o Fujifilm USB Device Managment Driver Windows has finished installing the software for this device To close this wizard click Finish Cancel Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 45 6 Installing and uninstalling the software 6 1 Installation Windows XP 6 1 2 Installation of USB Function driver 6 1 2 Installation of USB Function driver 46 1 Connect the computer and the Typhoon FLA 7000 with a USB cable and turn ON the power switch of Typhoon FLA 7000 Result The scanner will automatically be detected by the computer In the Found New Hardware Wizard dialog choose No not this time and click the Next button Found Mew Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on Pour computer on the hardware installation CD or on the Windows Update Web site with pour permission Read our privacy policy Can Windows connect to Windows Update to search for software Yes this time on
43. ly Yes now and every time connect a device Click Next to continue Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 1 Installation Windows XP 6 1 2 Installation of USB Function driver 3 Insert the installation CD select Install the software automatically Recommended and click the Next button Found New Hardware Wizard This wizard helps you install software for FUJIFILM FLA F000 J IF your hardware came with an installation CD lt 4 o floppy disk insert it now what do vou want the wizard to do Install from a list or specific location Advanced Click Next to continue 4 Click the Finish button to complete the installation Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for Fujifilm USB Devices Click Finish to close the wizard Cancel Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 47 6 Installing and uninstalling the software 6 1 Installation Windows xP 6 1 3 Installation of Typhoon FLA 7000 Control Software 6 1 3 Installation of Typhoon FLA 7000 Control Software 1 Insert the Typhoon FLA 7000 Control Software CD 2 Locate and double click the file Tyohoon FLA 7000 msi 3 Inthe Typhoon FLA 7000 InstallShield Wizard click the Next button ig Typhoon FLA 7000 InstallShield Wizard Welcome to the InstallShield Wizard for Typhoon FLA
44. m Center gt A aal 6 Sound Problem Reports a 8 Sync Center Printers Konni PN Dinu 53 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 1 Installation of USB control driver 3 Inthe Add Hardware dialog click the Next button Add Hardware Welcome to the Add Hardware Wizard This wizard helps you install driver software to support older devices that do not support Plug and Play and which are not automatically recognized by Windows fou should only use this wizard if you are an advanced user or you have been directed here by technical support A If your hardware came with an installation CD it is recommended that you click Cancel to close this wizard and use the manufacturer s CD to install this hardware To continue click Hest 4 Select Install the hardware that I manually select from a list Advanced and click the Next button Add Hardware The wizard can help you install other hardware The wizard can search for other hardware and automatically install it for you Or if you know exact which hardware model you want to install pou can select it from a list What do you want the wizard to doy C Search for and install the hardware automatically Recommended Install the hardware that manually select from a list Advanced 54 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the softwar
45. n Save File EtBr gel EtBr Low 256 High 53500 Curve Linear V Auto Range Scope Magnification 10 1 10 Q Q Stop Status Reading 48 8 Method EtBr Laser J 532nm Filter 0580 File Format Gel and TIFF Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 19 3 Reading fluorescent samples 3 4 Starting reading Note To stop reading before the scanning is completed click the Stop button e The area that has not been read will be saved as an image with a data value of 0 light intensity of O e The reading function is cancelled You cannot start reading again from the location where reading stopped 2 View information about the scan in the lower part of the Reader Scanning Condition Oo Low 0 High 65535 Curve Linear v V Auto Range Scope Magnification 10 1 10 A IA Status Reading 0 0 Method EtBr Laser J 532nm Filter 0580 File Format Gel and TIFF Funtion Displays the scan results of the 1st 2nd 3rd and 4th scan starting from the left Click on the tabs to switch the display Displays information related to the contents of the currently displayed scan 3 When you want to change the display parameters of the real time window refer to the explanations below and make settings Curve Exponential Select the type of tone curve from the pull down menu iw Auto Exponential Exponential The
46. n FLA 7000 Control Software User Manual 28 9607 63 AA 21 5 Lasers and filters other settings 5 Lasers and filters other settings 5 1 Registering laser and filter combinations Note Method settings are registered by a serviceman upon installation Under normal circumstances it is not necessary to register these settings You may register change and delete laser and filter combinations as described below 1 Click the Method button on the main window The Method Settings dialog box appears Method Settings Method Settings Method List Alexa Fluor 488 E4473nm Y520 Alexa Fluor 633 E3635nm R670 Alexa Fluor 680 Es670nm RR 10 CBB 3532nm 0580 Cy2 E3473nm LV520 Cy3 3532nm 0580 Cy5 Fy63snm JIRE70 DDAO Fa635nm JIR670 Deep Purple 69532nm 0580 EtBr Eas32nm 0580 FAM E3473nm LVS20 reit ETE ars Eirwen x Delete Edit Add Cancel OK Function Function Names of the registered laser and Laser Registered laser and filter filter combinations and combinations Filter Name Edit Click this button to edit laser and filter Cancel Click this button to delete laser and button combinations button filter combinations Add Click this button to register new laser button and filter combinations 28 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 5 Lasers and filters other settings 5 1 Registering laser and
47. n samples Refer to the following explanations of reading conditions when making settings a Image Folder Specify where to save the file after reading Click the Browse button and specify where to save the file after reading b File Name Enter the name of a file for saving image data Note You cannot start reading unless you enter a file name c Comment Enter a comment optional The comment is saved with the image as a file and can be viewed with the analyzing software d Method Select the method from the pull down menu that corresponds with the sample Method Digit473 v 473 Y520 2 1 22 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 4 Reading digitization samples 4 1 Setting the reading conditions Function The Method to be used for reading is displayed The contents of the laser and filter combination to be used for reading are displayed Note To use the Digitization mode the 473 nm laser must be selected and the Y520 filter must be registered or the SHG 532 nm laser must be selected and the 0580 filter must be registered If these conditions are not fulfilled the Reader Condition Settings window for the Digitization mode cannot be accessed e PMT You may set the voltage to be applied to the photo multipliers PMT as an integral value within the predetermined range PMT 500 V 500 1000 f Pixel Size Set the pixel size for reading Click to select from one of the four t
48. nel Options 2 Select Typhoon FLA 7000 and click the Remove button e Options 6 Installing and uninstalling the software 6 2 Uninstallation For Windows XP e i Printers and Other Hardware ae s T Ga aad Ge Reeve PA a Date Time Language and Regional Sounds Speech and Audio Devices Accessibility Options A y Performance and Maintenance Security Center Ny Add or Remove Programs Currently installed programs and updates Show updates i5 Hotfix for Microsoft NET Framework 3 5 SP1 KB958484 p Hotfix for Microsoft NET Framework 3 5 SP1 KB953595 F MSXML 6 0 Parser KB925673 PDF Complete E Privacy Manager for HP ProtectTools All Soundmax 4 Typhoon FLA 7000 Click here for support information To change this program or remove it from your computer click Change or Remove amp Typhoon FLA 9000 p Windows Genuine Advantage Validation Tool KB892130 Ee Windows Internet Explorer 8 Windows Internet Explorer 8 Software Updates Update For Windows Internet Explorer 8 KB971180 Ee Security Update for Windows Internet Explorer 8 KB969897 Windows Live OneCare safety scanner BP Windows XP Software Updates a Security Update For Windows XP KB923789 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA sort by Size 1 31MB Size 29 87MB Size 10 37MB Size Installed On 10 22 2008 Size 4 46MB Installed On 6 11 2009 Installed On 6 11 2009 Size 32 12
49. nstalling the software 6 3 Installation Windows Vista 6 3 2 Installation of USB function driver 4 Select Install this driver software anyway x Windows can t verity the publisher of this driver software gt Don t install this driver software You should check your manufacturer s website for updated driver software for your device Install this driver software anyway Only install driver software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal information See details 5 A successful installation message appears Click the Close button The software for this device has been successfully installed Windows has finished installing the driver software for this device Fujifilm USB Device 6 3 3 Installation of Typhoon FLA 7000 Control Software 1 Insert the Typhoon FLA 7000 Control Software CD 2 Locate and double click the file Typhoon FLA 7000 msi 62 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 3 Installation of Tyohoon FLA 7000 Control Software 3 Inthe Typhoon FLA 7000 InstallShield Wizard dialog click the Next button 4E Typhoon FLA 7000 InstallShield Wizard Ea Welcome to the InstallShield Wizard for Typhoon FLA The InstallShield R Wizard will install Typhoon FLA 7000 on your computer To
50. op FLA700 files Ainders E File Name EcL Plex C Comment PmT 620 Reader Settings Fluorescence iS Pixel Size Latitude d Method EA lst C25 um ig g E romy c 50 pm 100 pm o PMT 620 500 1000 Method fieval 2nd Sampling Area luorescence Stage A B C D E F G H I J K L M N O P 532nm40580 PMT 560 500 1000 f Method fievs X EZI E e2snmar670 PMT 750 500 1000 Mode amp Grid C Free C All CIDIE FIGIH I J KIL File Size about 4 12 MB x6 Reading Time about 6 m 30 s File Format Geland TIFF Top ND Filter Off D Start Scan Scan Mode Standard Click Top to return to the main window from the Fluorescence mode 2 Make these settings before reading fluorescent samples Refer to the following explanations of reading conditions when making settings a Image Folder Click the Browse button and specify where to save the file after reading Note When scanning multiple times the image data are saved in the folder with the name specified in File Name which is automatically created in the specified location b File Name Enter the name of a file for saving image data Note You cannot start reading unless you enter a file name Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 15 3 Reading fluorescent samples 3 1 Setting the reading conditions Note When the number of scans is between two and four the laser name and scan number are automatically added to the specified name
51. ple continuously carry out reading by following the above procedures Click the Return button to return to the first Reader Settings window Do not open the stage door of Typhoon FLA 7000 until the stage has completely returned If it is opened close it immediately When scanning finishes the Save as and Launch buttons become active but the Return button is grayed out until the stage has completely returned 6 Finish reading Before turning off the power of Typhoon FLA 7000 shut down the Typhoon FLA 7000 Control Software Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 21 4 Reading digitization samples 4 Reading digitization samples 4 1 Setting the reading conditions 1 Click the Digitization button The Reader Settings window for the Digitization mode is displayed Typhoon FLA 7000 File Edit Help Condition File Load Condition e Folder C Users Userdmin Desktop FLA700 files ee rowse b File Name Coomassie aig Comment C Reader Settings Digitization Pixel Size Latitude d Method cBB PMT 1000 500 1000 c25 pm C L4 E 532 0580 c50 pm Ls 100 pm C 200 um h Sampling Area Mode Grid C Free C All File Size about 2 54 MB x 2 Reading Time about 0 m 45 s File Format Gel and TIFF Start Scan Top ND Filter On gt Click Top to return to the main window from the Digitization mode 2 Make these settings before reading digitizatio
52. rance and Themes a Printers and Other Hardware e lt See Also gt Windows Update A Help and Support 7 Network and Internet Connections User Accounts GB Other Control Panel Add or Remove Programs S Date Time Language and Regional Options Options AN YD Sounds Speech and Audio Devices Accessibility Options y Performance and Maintenance Security Center Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 37 6 Installing and uninstalling the software 6 1 Installation Windows XP 6 1 1 Installation of USB Control Driver 38 Address 2 Click Add Hardware Printers and Other Hardware File Edit view Favorites Help Back 7 oi P Search Key Folders See Also We Add Hardware SA Display Sounds Speech and Audio Devices lt 3 Power Options 43 System Troubleshooters 2 Hardware 2 Printing 2 Home or Small Office Networking 3 Click the Next button Add Hardware Wizard G Printers and Other Hardware 28 Printers and Other Hardware Pick a task iew installed printers or fax printers Add a printer or pick a Control Panel icon Q Game Controllers lt Keyboard Phone and Modem Options moO en Printers and Faxes Scanners and Cameras Welcome to the Add Hardware Wizard This wizard helps you Install software to support the hardware you add to your computer Troubleshoot problems you may be having with
53. re when the Y520 is set in filter module position No 2 second from last CAUTION When removing the filter module make sure to press the Filter Module button and remove it after the window changes to the Filter Settings window If the filter module is removed with force the area where the filter comes in contact with the photo multipliers PMT will be damaged Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 29 5 Lasers and filters other settings 5 2 Filter module settings 5 2 1 Registering filters 1 Click the Filter Module button on the main window The following window appears and the filter module moves to a position where it can be taken out Filter Module Settings m Filter Module Settings 7 Filter List GP 0580 GBIR670 R710 0580 lt 4 R670 3 Ee 2 IP lt 4 HBy520 Filter Module Module1 1E F IP 2 0 Y520 4 3 R670 Load Filter Module 4 0580 6 Add Save Filter Module Cancel OK Function Click the Insert button to set a filter in the software It is also possible to use the mouse to drag and drop Function Click the Save Filter Module button to save filter combinations as a file Click the Remove button to remove a filter in the software e Click the Add button to register a new filter Click the Edit button to change the name or color of the displayed icon for the registered filter Default filters cannot b
54. recognize Typhoon FLA 7000 Please check connection and power e Warm up Typhoon FLA 7000 is in self diagnosis Please wait e Ready to use Ready 5 The main window of Typhoon FLA 7000 Control Software is displayed Mode Settings B Protein el eal See eee nT Te i H Fluorescence fen SF vora Typhoon FLA 7000 a CONTROL SOFTWARE imagination at work 2009 GENERAL ELECTRIC COMPANY ALL RIGHTS RESERVED Technical Support http Awww qelifesciences co j ttp iww qelifesciences com contact System Settings Method Status Ready Filter Module1 Laser U Filter Module ilg IP 532nm OK 2 Y520 473nm OK 3 R670 650nm OK BD Preferences 4 9 0580 635nm OK Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 1 2 Control Software main window 1 Preparations before use 1 2 Control Software main window P Typhoon FLA 7000 File Edit Help tohe Mode Settings I 4 amp Digitization Phosphorimaging Typhoon FLA 7000 System Settings peu wie x CONTROL SOFTWARE imagination at work 2009 GENERAL ELECTRIC COMPANY ALL RIGHTS RESERVED Technical Support http www qelifesciences com contact 4 Gy Method Status Ready e 3 Filter Moduls Laser 5 Fitermodue i Lae cl g r i ees YB oc so 8 No Name No Name 1 Phosphorimaging 6 Pr
55. rtant 58 Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 1 Installation of USB control driver 13 Click the Next button The wizard is ready to install your hardware Hardware to install ile Fujifilm USB Device Managment Driver To start installing your new hardware click Next 14 Click Install this driver software anyway l z 6 Windows can t verify the publisher of this driver software gt Don t install this driver software You should check your manufacturer s website for updated driver software for your device Install this driver software anyway l Only install driwer software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal information See details Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 59 6 Installing and uninstalling the software 6 3 Installation Windows Vista 6 3 1 Installation of USB control driver 15 Click the Finish button Completing the Add Hardware Wizard The following hardware was installed P Fujifilm USB Device Managment Driver Windows has finished installing the software for this device To close this wizard click Finish Cancel 6 3 2 Installation of USB function driver Note During software installations you may be asked to confirm your actions in a dialog with the text Windows n
56. the Multi stage Display of Multi stage and TP plugin Sampling Area AIBICIDIE FIGIH I JIK LIM IN O P MULTI STAGE amp TP PLUG IN AIBICIDIE FIGIH I JIK LIM N O P Select the scanning area on the shape of titer plate Mode Grid Free All Mode Grid C Free Bll 3 Reuse reading conditions Use the Save Condition button when saving the reading conditions in a file You may save reading conditions that are used frequently with this function and recall them later with the Load Condition button Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 3 Reading fluorescent samples 3 1 Setting the reading conditions Note When starting the Typhoon FLA 7000 Control Software the settings information from the previous session is displayed 3 2 Setting a fluorescent sample on the Fluor or Multi stage Set the fluorescent sample on the Fluor or Multi stage For instructions on setting the fluorescent sample see Typhoon FLA 7000 User Manual 3 3 Setting the Fluor or Multi stage on Typhoon FLA 7000 Set the Fluor or Multi stage on Typhoon FLA 7000 For instructions on setting the Fluor or Multi stage see Tyohoon FLA 7000 User Manual 3 4 Starting reading 1 Click the Start Scan button on the Reader Settings window to start reading Result The scanned area is displayed in the real time window as shown below The stage is read from the left to the right la Typhoon FLA 7000 Reader Scanning Conditio
57. ypes as shown on the left A sample with a smaller pixel size is analyzed more finely 200 100 50 m25 A T Short Reading Time Long D a Small Image File Size Large g Latitude Specify the dynamic range The dynamic range that can be detected is larger with L5 than with L4 If the signals of the sample are in the L4 range the density gradation is represented more finely if L4 is selected Typhoon FLA 7000 Control Software User Manual 28 9607 63 AA 23 4 Reading digitization samples 4 1 Setting the reading conditions h Drag and select the scanning area on the Flour stage Sampling Area Mode Grid C Free C All File Size about 2 21 MB Reading Time about TmQs Function Grid mode Select Grid mode to specify the reading area based on the 2 5 cm grid lines on the Fluor stage Free mode Select Free mode to arbitrarily specify the reading area All mode Select All mode to specify the entire Fluor stage as the reading area Reading Time Displays estimated time until reading finishes File size Displays size of the file Expressed as file size per test x number of scans Use the Save Condition button to save the reading conditions in a file You may save reading conditions that are used frequently with this function and recall them later with Load Condition Note When starting the Typhoon FLA 7000 Control Software the settings information from the previous session is displayed 4 2 Setting a d

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