Operating manual for universal centrifuge Z 306
Contents
1. Operating manual for universal centrifuge Z 306 O Hermle Labortechnik GmbH Z306 Instruction Manual docxlish 05 2012 Hermle Labortechnik GmbH Z306 Instruction manual docx Ta PRODUG TE DESCRIPTION anuario 3 1 1 Usage in accordance with safety standards 3 1 2 Brief COS CU IOI EE 3 1 3 OLIVET Eat Eeer 3 1 4 Operating and display elements canoas 2 LAT ECO D ue 3 1 5 Signs and indications of the centrifuge seen 3 AA e ee e a ga a EE OOO 3 192 Prod ct n ameplate Example eeen a a a men nt 4 1 53 Warning and information ION Sarria 5 1 5 4 Danger precautions and warranty nn inner 5 1 5 5 Following rules must strictly be adhered 10 ne 6 156 WV UNAM OPERA lo e 0 O EN GA Dang aaa aa ANANG 6 1 6 installation Of the centrifuge asian 7 1 6 1 Unpacking the centrifuge nn 7 102 ol e Eo e Eo OE OE A 7 A A A o e PR e OO E 7 1 7 STE 8 1 7 1 Access to mode Operating Data anna na a an aaa 8 UE Sound SIGA tumon EE 9 1 7 3 Volume pre selection of sound signal nn 9 1 7 4 Song selection for sound signal end of run 10 1 25 Keyboard sound UNO Oria rod 10 1 7 6 Call up operating data by skilled personnel or service engineer only 11 2 OPERATION ora aa 12 2 1 Mounting and loading angle rotor esconde 12 ZA DEE el OO ERES ER codann inga dad gak aa e agen 12 282 o E ne 12 2 19 Loading SWING OU OS EE 13 2 1 4 Loading and overloading of rotorg nn 14 23 ACM METO aoe decantado 14 2 2 B lleg 15 22 10 A o OOO SOU On 15 AA e A e2. M3 and 22x xx M4 In case of free program numbers it appears 0 Close the lid of the centrifuge Now proceed as already described to set all important run parameters For adaption of data press the key store 12 for approx 1 second As a result the word program M10 disappears As soon as the key store 12 is no longer anymore it reappears the word programm xx M10 the xx stands for the chosen program place If all program numbers are occupied you can take an old number that is not necessary anymore and just put in the new parameters All with number marked passages refer to figure 21 Figure 21 Recall of stored programs To recall stored programs press the key prog 11 while the lid is already closed Inside the display time A 3 appears programm M10 With the adjusting knob 1 you pre select the desired program number In the respective displays there will appear the stored values for that program If there is not the right rotor inside the centrifuge for the pre selected program in the display rpm rcf A 1 flashes the word rotor M3 At the same time the word FALSE and the stored rotor number 22x xx M4 will flashing by turns All with number marked passages refer to figure 22 Figure 22 O Hermle Labortechnik GmbH Z306 Instruction manual docx 19 20 2 5 3 Leaving program mode To leave the program mode just press the key
3. time 6 In the display time A 3 flashes the indication m s or h m depending on the previous setting To set the desired value use the adjusting knob 1 After exceeding of 59 min 50 sec the indication changes automatically into h m After exceeding of 99 hours 59 min the word cont appears in the display time A 3 That continuous run can only be interrupted by pressing the key stop 10 The time countdown as soon as the setted speed is reached The display shows always the remaining running time see figure 18 All with number marked passages refer to figure 18 Figure 18 Preselection of brake intensity and acceleration Through the key accel decel 5 this function is activated By pressing the key once the word accel M7 flashes in the display accel decel A 2 The desired acceleration can be pre selected by the adjusting knob 1 The value 0 is equivalent to the lowest and the value 9 to the highest acceleration By pressing the key accel decel 5 twice in the display accel decel A 2 indicates the word decel M8 Now the desired brake intensity can be pre selected by the adjusting knob 1 The value 9 is equivalent to the shortest and the value 0 to longest possible brake time All with number marked passages refer to figure 19 See table 4 acceleration and deceleration times APPENDIX P VIII There are shown the acceleratio
4. additional the word no M4 By pressing the key lid 7 you can release the lid of centrifuge As soon as the electromagnetic lid is completely released it appears the word open M2 Now you can open the lid of the centrifuge All with number marked passages refer to figure 16 rpm ref Figure 16 During the run you can call up the rotor type at any time by pressing the key lid 7 Lid lock The lid must be pressed down An electromagnetic lid lock closes the lid at the same time disappears the word open M2 As a sign that the centrifuge is ready for starting it appears in the display rom rcf A 1 the word close M1 Simultaneously it appears in that display the word rotor M3 as well as the code number of the rotor which is in the centrifuge i e nr 22x xx M4 With that all rotor specifically data like e g max speed acceleration etc are adopted All with number marked passages refer to figure 16 A ATTENTION Don t grip your fingers between lid and device or locking mechanism when closing the lid Hermle Labortechnik GmbH Z306 Instruction Manual docx 2 3 Preselection 2 3 1 2 3 2 Preselection of speed RCF value Through the key rpm rcf 4 this pre selection is activated By pressing the key once the word rpm M5 flashes By pressing the key once again the pre selection of the centrifugal forces may be chosen Then it appears the flashin
5. and scroll through it by the adjusting knob 1 The respective error codes appear in the display rom rcf A 1 Please look up in Table 5 error messages see APPENDIX S IX Here as well you must shortly switch off the centrifuge for changing to the normal program mode again romi rcf Figure 8 Hermle Labortechnik GmbH Z306 Instruction Manual docx 11 12 2 OPERATION 2 1 Mounting and loading angle rotor 2 1 1 2 1 2 Installation of rotors Clean the drive shaft as well as the collet with a clean grease free piece of cloth Place the rotor onto the drive shaft see figure 9 Take care that the rotor is fully installed onto the motor shaft Figure 9 Hold the rotor with one hand and secure the rotor to the shaft by turning the fixing nut clockwise Tighten fixing nut with enclosed allan key see figure 10 Figure 10 Figure 11 ATTENTION For safety always ensure that rotor fixing screw is tightened before each run see figure 10 Loading angle rotors Rotors must be load symetrically and with equal weight see figure 12 13 The adapter may only be load with the appropriate vessels The weight differences between the filled vessels are as low as possible to keep Therefore we recommend to weighting with a balance This reduces the wear of drive and the acoustic operating noise On each rotor is designated how large the maximum load per hole is lt is allowed to operate e g a 12 place ro
6. o In 15 2 3 A A A 16 231 PieselechonOrspeed e 16 CC deine Bd rie luet 16 2 3 3 Preselection of brake intensity and acceleration 17 2 4 AA AP e 18 2 5 A A 18 2921 SDN do o O ena NGNE NDENG Bk aa AN a ag kaang aan angadi a aa Bangga agin 18 EEGEN 19 2 5 3 Leaving lieler 20 Hermle Labortechnik GmbH Z306 Instruction manual docx Starting and stopping the centrifuge s s ssssssueunsnsununsnnunnnnnnununnnnunnnnnnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnunnnnnnnnnnnnnnnnnnnnn nnaman 20 2 6 1 Starting the centrifuge a o aa 20 202 110 5 Eege 20 imbalance detec lianas 21 3 MalnleNance ee e 22 Maintenance and CIEANING sancionada 22 A ee 22 3 1 2 Cleaning and disinfection Of the unit 23 3 1 3 Cleaning and disinfection of the rotor NANANG NANA NANANG 23 CERN edel vele fellt te CN 23 3 1 5 Disinfection of d ee 23 3 1 6 Ee ie 24 Service life of rotors round and rectangular buckets accessories mms 24 AA AP A 26 Error messages Cause SOMMOM nn aa NAN AGA aa AAN una NGNE aa NA aka benedentuin arten daneen 26 Survey of possible error messages and their solutions sr 26 42 1 Lid release during power failure Emergency Lid Release 26 4 2 2 Description of the error message system ins 27 5 Receipt of centrifuges to repair nnannsarnennennennennennennennennennnenennennnnnennennnnnnen 29 6 TRANSPORT STORAGE AND DISPOSAL naa eneenernennennensenneneeneens 30 HB AVN OO nne E E E 30 0 E 30 AAPP 30 7 APPEND
7. requirements The centrifuge should be installed on an even solid surface if possible on a laboratory cabinet table or some other solid vibration free surface During centrifugation the centrifuge must be placed in a way that there is a minimum space of 30 cm on each side of the unit according to the standards EN 61010 2 020 Do not place the centrifuge next to a window or a heater where it could be disposed to excessive heat as the performance of the unit is based on an ambient temperature of 23 C Installation Follow these steps e Check whether power supply corresponds with the one named on the manufacturer s rating label which is mounted on the rear panel e The line voltage circuit braker is max 10 A type K slow release for commonly used instruments e In case of emergency there must be an emergency switch off installed outside the room in order to disconnect the power supply of the unit e Connect the centrifuge with the mains The socket for the power cord must be easy to reach respectively easy to disconnect Switching it on using the mains power switch Open the lid by using the button LID e Remove the transport securing device of the motor Hermle Labortechnik GmbH Z306 Instruction Manual docx 1 7 Basic adjustments 1 7 1 At commissioning of the centrifuge you have the options to make the following basic settings Acustic signal turn on oft Keyboard sound turn on off Volume pre selecti
8. switch off the centrifuge for a short while rpmircf Figure 6 Keyboard sound turn on off Proceed as described under point 1 7 1 to enter this program mode and then press the key accel decel 5 In the display accel decel A 2 flashes the word service Now select the letter D with the adjusting knob 1 As a result appears in the display rom rcf A 1 the word ON BEEP After pressing the key rpm rcf 4 you can turn the keyboard sound On or Off with the adjusting knob 1 see figure 7 After you have stored the settings see 1 7 1 you changed to the normal program mode again by switch off the centrifuge for a short while remircf Figure 7 Z306 Instruction manual docx O Hermle Labortechnik GmbH 1 7 6 Call up operating data by skilled personnel or service engineer only In the mode Basic Adjustments you can call up the operating data of the centrifuge Please proceed as described under point 1 7 1 to enter this program mode Press the key accel decel 5 In the display accel decel A 2 flashes the word service With the adjusting knob 1 the different information can be called up A previous starts of the centifuge H previous operating hours S software version r Converter software E list of previous error massage h running time of the motor The list of the last 99 error messages can be looked over by pressing the key rpm rcf 4
9. 0 72 79 14 140 06 705 008 220 72 80 14 14 0 06 705 009 220 72 84 04 146 0 705 010 220 72 85 04 140 06 705 012 2207262 140 06 705 015 2207265 140 06 705 016 2207266 140 06 2207288 139 07 704004 004 220 56 55 04 704 005 220 56 56 04 With 220 96 12 04 107 220 96 12 04 With 220 96 08 04 220 96 05 04 With of 220 96 08 04 701 011 220 96 06 04 603 000 220 97 04 04 aa Z306 Instruction manual docx Hermle Labortechnik GmbH Table 6 part 2 Radius correction Rotor no Adapter Tube Drawing no Radius cm Correction rack Order no cm 8 out rotor 626 003 221 12 09 03 14 8 221 12 ES 02 a 626002 221 12 07 03 we 02 626 004 221 12 11 03 145 03 626 005 221 12 13 03 142 06 626 006 221 12 06 03 14 2 06 626 007 221 12 12 03 140 08 06 26 008 008 221 12 16 14 2 626 009 2211217 142 06 626 010 2211218 143 05 eson aen 10a 0a 03 626 013 013 221 12 21 14 5 626 014 2211222 99 49 626 015 2211224 116 32 221 01 02 EEEN A 221 17 01 E E 234 11 0 56 56 0 3 4 a 221 19 01 10 9 701 000 225 52 71 04 8 AA A 9 7 CU ue 10 6 ET A 8 9 EEE ECE 221 24 01 maa OO 221 25 01 5 133 03 Hermle Labortechnik GmbH Z306 Instruction manual docx A Table 7 Abbreviations used Symbol Abbreviation RZB rc
10. 1 17 VO3 34 221 19 VO2 20 221 24 V02 95 in seconds Accelertaion time Decelertaion time VIII Z306 Instruction Manual docx Hermle Labortechnik GmbH A Table 5 Error messages orto esei Imbalance arose Imbalance switch has been activated for longer than 5 seconds Transponder in the rotor is defective 14 Leap of speed is too big between two measurements CLOSE lid Open lid while motor is runninc MI Imbalance sensor is defective Communication with frequency converter disturbed during start 03 20 an communication with frequency converter disturbed during stop a2 Sort inn tregueney convener 8 JUnderotage equeney comentar a overotage requeney convener as over temperatura frequency coment overtomperatemetor OOOO a8 Timeout between control unit and requeneycomvener as Joiner or equeney cometer CN oee 20 oor is notaowedinthiscontfuge aen Rotrisnotdeected 7 7 O Hermle Labortechnik GmbH Z306 Instruction manual docx IX Tabelle 6 Radius correction Rotor no Swing out rotor 220 72 VO4 Angle rotor 220 87 VO9 220 87 V10 Angle rotor 220 96 VO4 Angle rotor 220 97 VO4 Swing out rotor 220 50 V08 Adapter Tube Drawing no Radius Correction rack Order no cm cm Sem a 605 000 001 220 72 06 03 142 04 705 002 220 72 73 04 140 06 705 003 220 72 74 04 140 06 705 005 220 72 76 04 137 09 705 007 22
11. 34 C Z306 Instruction manual docx O Hermle Labortechnik GmbH 3 1 2 3 1 3 3 1 4 3 1 5 Cleaning and disinfection of the unit Open the lid before you turn off the unit Disconnect it from the power supply Open the rotor nut by turning the rotor key counter clockwise 1 2 3 Remove the rotor A For cleaning and desinfection of the unit and the rotor chamber using the above mentioned cleaner 5 Clean all accessible areas of the device and its accessories including the power cord with a damp cloth 6 Wash the rubber seals and rotor chamber thoroughly with water 7 Rub the dry rubber seals with glycerol or talc to prevent these to becoming brittle Other components of the unit e g the lid lock motor shaft and rotor must not be greased 8 Dry the motor shaft with a soft dry and lint free cloth 9 Control the unit and accessories for damage Remove at least every six months adherent dust from the ventilation slots in the centrifuge by using a soft brush Cleaning and disinfection of the rotor 1 Clean and disinfect the rotors rotor lids and adapters with the above mentioned cleaner 2 Use a bottle brush to clean and disinfect the rotor bores 3 Rinse the rotors rotorlid and adapter with clear water Particular the drillings of angle rotors A For drying of the rotors and accessories set them on a towel Place the angle rotors with bores down to dry them to 5 Dry the rotor cone with a soft dry an
12. Display field acc dec Display field time The following picture shows the individual elements of the LCD display Messages logos of the display fields M1 M2 M3 M4 M5 M6 M7 1 5 Signs and indications of the centrifuge 1 5 1 General close open rotor Rotor No rpm rof accel M8 M9 M10 M11 M12 M13 M10 M11 M9 decel radius program error Service hms Y Instructions for disposal see chapter 6 P 28 EA gt Direction of rotation clockwise rotation for the rotor drive Hermle Labortechnik Z306 Instruction Manual docx 3 Reference for loading rotors Company address TYPE REF SN MAX Drehzahl KIN EN U 1 f LU CE 1 5 2 Product nameplate Example Hermle Labortechnik GmbH Siemensstr 25 D 78564 Wehingen TYPE Z36HK REF 302 00 VO1 SN 58105001 y 2010 MAX DREHZAHL 30000 1 min KIN EN 50880 Nm Ur 120 VAC 15 8A 50 60Hz P 1 6KW Made in Germany AR C gt Hermle Labortechnik GmbH Siemensstr 25 D 78564 Wehingen Type designation of the product Order no of the product Serial number of the product Date of manufacture max allowed speed of the unit max kinetic energy with corresponding rotor Allowable voltage max current frequency Electrical input power Before operating read the operating manual Labeling standards and guidelines th
13. IX a eee XXXI EC Conformity Declaration inner IV CADET TECHNICAL RE V EWEG VI Table 3 Max speed and RCF values for permissible rotors E VII Table 4 Acceleration and deceleration mes VIII EA A aa daa o eg IX E 0 Radius dl y een X Table 7 Abbreviations used iii XII Redemption form Decontamination certificate ss XIII Z306 Instruction manual docx Hermle Labortechnik GmbH 1 PRODUCT DESCRIPTION 1 1 Usage in accordance with safety standards A This symbol indicates safety instructions and points to potential dangerous situations Before using the centrifuge the first time please read the operating manual Failure to follow these instructions can result in personal injury an property damage Intended use includes the observance of all instructions in the instruction manual and carrying out inspection and maintenance This Hermle centrifuge was designed only for the separation of materials or mixtures with different density specifically for the preparation and processing of samples from the human body in context of an in vitro diagnostic use to allow the use of in vitro diagnostic in accordance to its intended purpose The designated device and its accessories listed in the technical documentation for this device are in accordance with Directive 98 79 EC on in vitro diagnostic medical devices Hermle centrifuges are intended exclusively for indoor use and for use by qualified personnel Only Hermle original ro
14. aler Hermle Labortechnik GmbH Z306 Instruction manual docx V Table 2 Permissible net weight VI Rotor number 220 50 VO8 220 72 V06 220 87 V09 220 87 V10 220 96 V04 220 97 V04 221 12 V03 221 16 VO3 221 17 V03 221 19 V02 221 24 V02 221 25 V03 3500 min 1 4000 min 1 13500 min 1 13500 min 1 6000 min 1 6000 min 1 4500 min 1 4500 min 1 12000 min 1 4500 min 1 3250 min 1 5000 min 1 Max speed Permissible net weight 4x 200g 4x 465 g 24 x 3 4 9 24 x 3 4 9 12x 25g 6x 72g 4 x 340 g 2x 400g 30x 3 4 g 30 x 32 9 2x709 6x110g Z306 Instruction Manual docx Hermle Labortechnik GmbH 220 50 V08 220 72 V06 220 87 V09 220 87 V10 220 96 V04 220 97 V04 221 12 V03 221 16 V03 221 17 V03 221 19 V02 221 24 V02 221 25 V03 Hermle Labortechnik GmbH 3500 min 1 4000 min 1 13500 min 1 13500 min 1 6000 min 1 6000 min 1 4500 min 1 4500 min 1 12000 min 1 4500 min 1 3250 min 1 5000 min 1 Table 3 Max speed and RCF values for permissible rotors RCF value 1424 xg 3885 XQ 17319 xg 17319 xg 4185 xg 3823 xg 3350 xg 2716 Xg 15133 xg 2830 xg 1700 xg 3801 xg Z306 Instruction Manual docx VI Table 4 Acceleration and deceleration times Acceleration values Deceleration values Rotor number level 0 level 9 level 0 level 9 14 19 220 50 V08 220 72 VO6 14 17 220 87 VO9 22 39 220 87 V10 25 39 220 96 VO4 13 13 220 97 V04 13 13 221 12 VO3 14 19 221 16 VO3 25 26 22
15. at are considered Z306 Instruction manual docx O Hermle Labortechnik GmbH 1 5 3 Warning and information signs Warning Four carrier must be used at al Four carrier must be used at all times on four place swing out rotors or TETRA damage will occur to the centrifuge Such damage will not be covered rotors or damage will occur to the centrifuge Such damage will not be under the product warranty covered under the product warranty Attention Check the fastening of the rotor nut before each run Vor manueller Entriegelung oder ffnen des Geh uses Netzstecker Ziehen TAKE OFF MAINS PLUG before opening Take off mains plug before opening the housing or the emergency release the housing or the emergency release RETIREZ LE CORDON avant toute intervention a l interieur de l appareil 1 5 4 Danger precautions and warranty This device may only be operated by trained specialist stuff They must have carefully read the operating manual and be familiar with the function of the device To protect people and environment the following precautions should be observed Hermle Labortechnik GmbH During centrifugation the presence of people and the setting up of hazardous materials is prohibited within 30 cm around the centrifuge according to the regulations of EN 61010 2 020 The HERMLE Z 306 is non explosion proof and must therefore not be operated in explosion endangered areas or locations Centrifugation of f
16. contamination of the listed parts confirm the proper decontamination Company dept place and date Hermle Labortechnik GmbH Z306 Instruction manual docx XIII Signature of the authorized person XIV Z306 Instruction manual docx O Hermle Labortechnik GmbH O Hermle Labortechnik GmbH Z306 Instruction manual docx Labnet Labnet International Inc 31 Mayfield Ave Edison NJ 08837 lt USA e US toll free 888 LABNET1 fax 732 417 1750 International lt phone 1 732 417 0700 fax 1 732 417 1750 website http www labnetinternational com email labnetinfo corning com Technical modification rights reserved OHERMLE Labortechnik GmbH 2012 Z306 Instruction manual docx O Hermle Labortechnik GmbH
17. d lint free cloth and look for damage Do not grease the rotor cone Disinfection of aluminium rotors In case of infectious material spilling into the centrifuge the rotor and rotor chamber have to be disinfected right after the run Rotors may be autoclaved at a maximum temperature of 121 C Disinfection of PP rotors Autoclaving The recommended time for autoclaving 15 20 min at 121 C 1 bar ATTENTION The sterilization time of 20 min must not be exceeded Sterilization again and again will cause reduction of the mechanical resistance of the plastic material Before the autoclaving the PP rotor and adapter must thoroughly be cleaned to avoid the burning in of dirty residues You can disregard the consequences of some chemical residues to plastic materials at ambient temperatures But at the high temperatures of the autoclaving those residues may corrode and O Hermle Labortechnik GmbH Z306 Instruction manual docx 23 destroy the plastic The objects must be thoroughly washed up with distilled water after the cleaning but before the autoclaving Residues of any cleaning liquids may cause fissures whitening and stains Gassterilization Adapters bottles and rotors may be gassterilized with Ethylenoxyd According to the duration of the application you may give long enough an airing to the items after the sterilization and before using them again ATTENTION Because the temperature may rise during the sterilization
18. ding and overloading of rotors All approved rotors are listed with their maximum speed and maximum filling weight in table 2 permissible net weight see APPENDIX P VI The maximum load permitted for a rotor which is determined by the manufacturer as well as the maximum speed allowed for this rotor see label on rotor must not be exceeded The liquids the rotors are loaded with should have an max homogeneous density of 1 2 g ml or less when the rotor is running at maximum speed In order to spin liquids with a higher density the speed has to be reduced according to the following formula 1 2 Reduced speed neg higher danz y max speed Nmax of the rotor Example ie Ned A x 4 000 3 360 rpm If In case of any questions please contact the manufacturer 2 1 5 Removing the rotor Untighten the rotor fixing nut complete 2 screw over the stiff point and lift the rotor vertical out of the centrifuge See figure 10 and 11 Z306 Instruction manual docx O Hermle Labortechnik GmbH 2 2 Lid 2 2 1 2 2 2 Lid release After the run respectively closing the lid of the centrifuge it appears in the display rpm rcf A 1 the word close M1 If there is a rotor in the centrifuge it appears additional the word rotor M3 as well as the code number of the respective rotor which is in the centrifuge e 221 28 M4 If there is no rotor in the centrifuge it flashes the word rotor M3 and
19. e Labortechnik GmbH 4 2 2 Description of the error message system The error message error M11 is shown in the time A 3 display see figure 27 Detailed information about possible error messages are in table 5 error messages see Appendix P IX M11 Figure 27 Hermle Labortechnik GmbH Z306 Instruction Manual docx 27 28 Z306 Instruction manual docx O Hermle Labortechnik GmbH 5 Receipt of centrifuges to repair A Health risk from contaminated equipment rotors and accessories In case of returning the centrifuge for repairing to the manufacturer please notice the following The centrifuge must be decontaminated and cleaned before the shipment for the protection of persons environment and material Decontamination certificate at goods return delivery see APPENDIX P XIV We reserve the right to accept contaminated centrifuges Further on all costs occurred for the cleaning and disinfection of the units will go to the debit of the customers account Hermle Labortechnik GmbH Z306 Instruction Manual docx 29 6 TRANSPORT STORAGE AND DISPOSAL 6 1 Transport Before transporting take out the rotor Only transport the unit in the original packaging Use a transport aid for transporting over longer distances to fix the motor shaft 25 bis 60 C 10bis75 30 bis 106 kPa 6 2 Storage During storage of the centrifuge the following environmental conditions must be observed in
20. f relative centrifugal force Polypropylen Polycarbonat De acceleration MN deceleration XII Z306 Instruction manual docx Hermle Labortechnik GmbH Redemption form Decontamination certificate Decontamination certificate at goods return delivery Enclose at all returns of equipment and assemblies absolutely The completely full declaration about the decontamination is prerequisite for the assumption and further processing of the return lf no corresponding explanation is enclosed we carry out decontamination with costs at your expense Surname last name xX Organization company iS Street 5 23 ZIP CODE place a 0 Telephone fax ze E Mail a Le Decontaminated feelend Deseripton Commen object Are these parts listed above in touch with the following substances Health endangering watery solutions buffers acids alkalis O Yes O No Potentially infectious agents enen nenn nnen nenne O Yes O No Organic reagents and solvent oooococcccccccccccnconcnocononcnnnnnnnconnnnnnnnnnrnnnnnnnnns O Yes O No Radioactive substances nanne ennen eeen eenen Do 6 0 y O Yes O No Health endangering proteins oon vennen venennnn venen annen sneren O Yes O No Ri TEE O Yes O No These substances have reached the equipment assembly O Yes O No Which one if yes Description of the measures for the de
21. from first use Condition for the operating life Proper use damage free condition recommended care 24 Z306 Instruction manual docx O Hermle Labortechnik GmbH O Hermle Labortechnik GmbH Z306 Instruction manual docx 25 4 Trouble Shooting 4 1 Error messages Cause Solution The error messages are listed to help localize possible errors faster The diagnose referred to this chapter may not always be the case as they are only theoretically occurring errors and solutions Always please keep us informed about any kind of error occurring which is not listed in this chapter Only through your information we are able to improve and complete this operation manual Many thanks in advance for your support HERMLE Labortechnik GmbH 4 2 Survey of possible error messages and their solutions 4 2 1 Lid release during power failure Emergency Lid Release In case of power failure or malfunction the lid of the centrifuge can be opened manually in order to protect your samples Please proceed as follows see Figure 26 A Switch the centrifuge off and unplug the power cord wait until the rotor stands still this may take several minutes At the right side of the centrifuge there is a plastic stopper Figure 26 Remove this stopper which is connected to the lid lock horizontally from the housing until the centrifuge lid opens e Now open the lid of the centrifuge Figure 26 26 Z306 Instruction Manual docx Herml
22. g word ref M6 You can set the desired values with the adjusting knob 1 In the display A 1 the regulated value is shown permanently before during and after the run All with number marked passages refer to figure 17 rrm rcf rmircf Figure 17 As long as no rotor is inserted the speed is adjustable between 200 rpm and maximum revolution of the centrifuge lf there is a rotor in the centrifuge the speed can only be pre selected until the maximum permissible revolution of that rotor It is the same with the pre selection of the RCF value The setting range is between 20 xg and the maximum permissible centrifugal force of the rotor See table 3 max speed and RCF values for permissible rotor see APPENDIX P VII There are listed all important values ATTENTION Please also check the maximum permissible revolutions of your test tubes Producer Indication Preselection of running time The running time can be pre selected in three different ranges from 10 seconds up to 99 hours 59 minutes 1 Range from 10 seconds up to 59 minutes 50 seconds in steps of 10 seconds 2 Range from 1 hour up to 99 hours 59 minutes in steps of 1 minutes 3 Range continuous run cont which can be interupted by the key stop 10 Z306 Instruction manual docx O Hermle Labortechnik GmbH 2 3 3 The running time can be pre selected with the lid open or closed To activate the setting of the running time press the key
23. gure 4 After you have stored the settings you changed to the normal program mode again by switch off the centrifuge for a short while Figure 4 Volume pre selection of sound signal Proceed as described under point 1 7 1 to enter this program mode and then press the key accel decel 5 In the display accel decel A 2 flashes the word service Now select the letter U with the adjusting knob 1 As a result appear in the display rom rcf A 1 the words Vol 0 9 Sound After pressing the key rpm rcf 4 you can adjust the desired volume between 0 low and 9 loud with the adjusting knob 1 see figure 5 After you have stored the settings see 1 7 1 you changed to the normal program mode again by switch off the centrifuge for a short while Figure 5 O Hermle Labortechnik GmbH Z306 Instruction Manual docx 10 1 7 4 Song selection for sound signal end of run Proceed as described under point 1 7 1 to enter this program mode and then press the key accel decel 5 In the display accel decel A 2 flashes the word service Now select the letter Q with the adjusting knob 1 As a result appears in the display rpm rcf A 1 the word SonGo Sound After pressing the key rom rcf 4 you can select a song with the adjusting knob 1 see figure 6 After you have stored the settings see 1 7 1 you changed to the normal program mode again by
24. l halogenated hydrocarbons A Cleaning units rotors accessories Turn the device off and disconnect it from the power supply before you begin any cleaning or disinfecting Do not pour liquids into the housing interior Do spray disinfectant on the device Thorough cleaning not only has its purpose in hygiene but also in avoiding corrosion based on pollution In order to avoid damaging anodized parts such as rotors reduction plates etc only pH neutral Detergents with a pH value of 6 8 may be used for cleaning Alkaline cleaning agents pH value gt 8 must not be used After cleaning please ensure all parts are dried thoroughly either by hand or in a hot air cabinet max Temperature 50 C It is necessary to coat anodized aluminium parts with anti corrosion oil regularly in order to increase their life spans and reduce corrosion predisposition Due to humidity or not hermetically sealed samples condensate may be formed The condensate has to be removed from the rotor chamber with a soft cloth regularly A The maintenance procedure has to be repeated every 10 to 15 runs but at least once a week Connect the unit to the power supply after the equipment is completely dry Do not carry out disinfection with UV beta and gamma raysor other high energy radiation Metal rotors can be autoclaved Rotor lid and adapters can also be autoclaved max 121 C 20 min The tube racks are made of PP and can not be autoclaved at 1
25. lammable explosive radioactive or such substances which chemically react with high energy is strictly prohibited The final decision on the risks associated with the use of such substances is the responsibility of the user of the centrifuge Never spin toxic or pathogenic material without adequate safety precautions Le centrifugation of buckets tubes without or with defective hermetic sealings is strictly prohibited The user is obliged to perform appropriate disinfection procedures in case dangerous substances have contaminated the centrifuge and or its accessories When centrifuging infectious substances always pay attention to the General Laboratory Precautions If necessary contact your safety officer It is prohibited to run the centrifuge with rotors other than listed for this unit Under no circumstances open the lid of the centrifuge while the rotor is still running or rotating with a speed of gt 2m s Z306 Instruction Manual docx 1 5 5 Following rules must strictly be adhered to Do not operate the centrifuge in case it is not installed correctly Do not operate the centrifuge when dismounted e g without housing Do not run the centrifuge when mechanical or electrical assembly groups have been tampered with unauthorized persons Do not use accessories such as rotors and buckets which are not exclusively approved by HERMLE Labortechnik GmbH except commercially available centrifuge tubes made of glass o
26. n and deceleration times for the acceleration and deceleration stages 0 to 9 for permissible rotors O Hermle Labortechnik GmbH Z306 Instruction manual docx 17 18 Figure 19 2 4 Radius correction If you use adapters or reducers it could change the centrifugal radius of the respective rotor In that case you can correct the radius manually Please proceed as follows Press the key time 9 and the key prog 11 at the same time and hold them In the display time A 3 appears the word radius M9 By the adjusting knob 1 you can pre select then the respective radius correction see table 6 APPENDIX P X in steps of 0 1 cm As soon as you have set a radius correction the word radius M9 appears This hint is as long visible as you put the radius correction back to 0 again All with number marked passages refer to figure 20 Figure 20 2 5 Program 2 5 1 Storage of programs You can store up to 99 runs with all relevant parameters incl the used rotors You can use any free program number and call it up again Put the needed rotor into the centrifuge By pressing the key prog 11 in the display time A 3 appears the word program With the adjusting knob 1 you can chose the desired program number Z306 Instruction manual docx O Hermle Labortechnik GmbH 2 5 2 If a program number is already occupied in the display rpm rcf A 1 will appear the words rotor
27. on The rotor decelerates to stand still When in the display time A 3 the word error M11 together with the number 01 appear the weight difference of the samples is too huge Weigh out the samples exactly Load the rotor as described in chapter 2 1 1 and 2 1 2 When inside the display time A 3 the word error together with the number 02 see figure 25 appear there could be following reasons The imbalance switch is defective Figure 25 O Hermle Labortechnik GmbH Z306 Instruction manual docx 21 22 3 Maintenance 3 1 Maintenance and cleaning 3 1 1 General Care Maintenance of the centrifuge is confined to keeping the rotor the rotor chamber and the rotor accessories clean as well as to regularly lubricating the rotor insert bolts of a swing out rotor if available The most suitable lubricant is the offered HERMLE High TEF oil Order no 34 5147 Lubricants containing molycote and graphite are not allowed Please pay special attention to anodized aluminium parts Breakage of rotors can be caused even by slightest damages In case of rotors buckets or tube racks getting in touch with corrosive substances the concerned spots have to be cleaned carefully Corrosive substances are for instance alkalis alkaline soap solutions alkaline amines concentrated acids solutions containing heavy metals water free chlorinated solvents saline solutions e g salt water pheno
28. on of sound signal Song selection of sound signal end of run Access to mode Operating Data If the centrifuge is still turned off press simultaneously the keys time 6 and lid 7 and turn on the main switch of the centrifuge Now release both keys again As a result a display test is excecuted for approx 5 seconds All possible indications will appear at the same time see Figure 2 Figure 2 Attention Please notice that you must enter the program as described under point 1 7 1 to change the adjustments of the points 1 7 2 1 7 6 After you have stored the settings you change the normal program mode again by switch off the centrifuge for a short while All changed settings must be confirmed by the key start 9 As an optical confirmation appears the word store in the display rpm rcf A 1 Only then the pre selections are valid see figure 3 Z306 Instruction manual docx Hermle Labortechnik GmbH Figure 3 Sound signal turn on off Proceed as described under point 1 7 1 to enter this program mode and then press the key accel decel 5 In the display accel decel A 2 flashes the word service Now select the letter L with the adjusting knob 1 As a result appear in the display rpm rcf 4 the words On Sound If you press the key rpm rcf 4 now the word On flashes and you can switch off the sound with the adjusting knob 1 see fi
29. prog 11 Then inside the display time appears the word Programm Set the display to programm M10 with the adjusting knob 1 All with number marked passages refer to figure 22 2 6 Starting and stopping the centrifuge 2 6 1 2 6 2 Starting the centrifuge You can start the centrifuge either with the start key 9 or the quick key 8 By the start key 9 you can start stored runs or runs with manually pre selected parameters When the respective pre selected running time has ended then the centrifuge will stop automatically By the quick key 8 you can start runs which will last just a few seconds By pressing the quick key 8 the centrifuge accelerates up to the pre selected revolution In the display time A 3 the passed running time is indicated from the date of pressing the quick key 8 By releasing the quick key 8 the centrifuge stops and the running time is indicated until the opening of the lid All with number marked passages refer to figure 23 Figure 23 The STOP key By the stop key 10 see figure 24 you can interrupt the run at any time After pressing the key the centrifuge decelerates with the respective pre selected intensity down to stand still Figure 24 Z306 Instruction Manual docx Hermle Labortechnik GmbH 2 7 Imbalance detection In case of the rotor not being equally loaded the drive will turn off during accelerati
30. r plastic Do not spin extremly corrosive substances as they may cause material damages and impair mechanical resistance Do not operate the centrifuge with rotors or buckets which show any signs of corrosion or mechanical damage The manufacturer is responsible for safety and reliability of the centrifuge only if the unit is operated in accordance with this instruction manual modifications repairs or other adjustments are performed by HERMLE authorized personnel and the electrical installation of the related location corresponds to the IEC regulations 1 5 6 Warranty The centrifuge has been subjected to thorough testing and quality controls In the unlikely case of any manufacturing faults occurring the centrifuge and rotors are covered by warranty for a period of two years from date of delivery This warranty becomes invalid in case of mishandling damage and negligence and further in case of usage of inappropriate spare parts and or accessories or unauthorized modification of the unit Technical modification rights are reserved by the manufacturer in respect to technical improvement Z306 Instruction manual docx O Hermle Labortechnik GmbH 1 6 Installation of the centrifuge 1 6 1 1 6 2 1 6 3 Unpacking the centrifuge Model Z 306 is supplied in a carton Remove the strap retainer open the carton and remove the centrifuge The instruction manual must always be kept with the centrifuge Space
31. rotors adapters and bottles must not be closed respectively must be totally unscrewed Chemical sterilization Bottles adapters and rotors may be treated with the usual liquid disinfectants ATTENTION Before applying any other cleaning resp Decontamination method than recommended by the manufacturer contact the manufacturer to ensure that it will not damage the unit or the rotor 3 1 6 Glass breakage With high g values the rate of glass tube breakage increases Glass splinters have to be removed immediately from rotor buckets adapters and the rotor chamber itself Fine glass splinters will scratch and therefore damage the protective surface coating of a rotor If glass splinters remain in the rotor chamber fine metal dust will build up due to air circulation This very fine black metal dust will extremely pollute the rotor chamber the rotor the buckets and the samples If necessary replace the adapters tubes and accessories to avoid further damages Check the rotor bores regulary for residues and damages ATTENTION Please check the relevant specifications of the tubes centrifuges with the manufacturer 3 2 Service life of rotors round and rectangular buckets accessories Rotors and rotor lid made of aluminium or stainless steel have a operating life of max 7 years from first use Transparent rotor lids and caps made of PC or PP as well as rotors tube racks and adapters of PP have a maximum service life up to 3 years
32. s used 2 2 222222222222022002022022an20n2u0nu2n00n00nnnnnnnnnnnnnnn nun nun nun nnn ann nn XII Redemption form Decontamination Certificate nnannensennennennenn XIII Hermle Labortechnik GmbH Z306 Instruction manual docx 31 EC Conformity Declaration EG Konformit tserkl rung EC Conformity Declaration CE Hermle Labortechnik GmbH Siemensstr 25 D 78564 Wehingen Germany Das bezeichnete Produkt entspricht den einschl gigen grundlegenden Anforderungen der aufgef hrten EG Richtlinien und Normen Bei einer nicht mit uns abgestimmten Anderung des Produktes oder einer nicht bestimmungsgem en Anwendung verliert diese Erkl rung ihre G ltigkeit The Product named below fulfills the relevant fundamental requirements of the EC directives and standards listed In the case of unauthorized modifications to the product or an unintended use this declaration becomes invalid Produkttyp Product type Laborzentrifugen mit Zubeh r nach IVD andere Laboratory centrifuge with accessories to IVD others Typenbezeichnung Typ designation Z 100 M Z 160 M Z 206 A Z 233 M 2 Z 306 Z 326 Z 366 Z 383 Z 400 Z 513 Z 216 MK Z 32 HK Z 326 K Z 36 HK Z 383 K Z 400 K Z 513 K Einschl gige EG Richtlinien Normen Relevant EC directives standards 98 79 EG 2006 95 EG 2004 108 EG EN 61 010 1 EN 61 010 2 020 EN 61 010 2 101 EN ISO 14971 2007 DIN ISO 13485 HERMLE Alexander Hermle Ge
33. sch ftsf hrer Managing Director Wehingen den 01 01 2012 IV Z306 Instruction manual docx O Hermle Labortechnik GmbH e Table 1 Technical Data Weight without rotor 30 kg max speed 13500 min max volume 4 x 100 ml max RCF 18625 x g allowable density 1 2 kg dm allowable kinetic energy 5595 Nm Rotor 221 17 VO3 Mains power connection AC 230 V 50 Hz 1 ph 120 V 60 Hz 1 ph Voltage fluctation 10 10 Current consumption 193 2 4 Power consumption 0 24 KW 0 3 KW Radio interference VDE 0871 radio interference B Audit requirement BGR 500 no Ambient conditions EN IEC 61010 1 Environement for indoor use only High Use up to an altitude of 2000 m above MSL Ambient temperature 2 C up to 35 C Max relative humidity Max relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity up to 35 C Overvoltage category IEC 60364 4 443 Degree of contamination 2 Class of protection Gg Not suitable for use in hazardous environements EMV EN IEC FCC Class B EN IEC FCC Class B Interference emission noise immunity 61326 1 61326 1 Category B Category B Noise level depending on the rotor lt 63dB A Write from operator Inventory No Monitoring Nr Environement Maintenance contract HERMLE Labortechnik GmbH or dealer service office Siemensstrasse 25 responsible service office 78564 Wehingen Tele aa Fax 49 7426 96 22 49 responsible de
34. tor with 2 or 4 loaded tubes only But the loaded borings must be opposite each other Z306 Instruction manual docx O Hermle Labortechnik GmbH Figure 12 wrong Figure 13 correct 4 tubes 2 1 3 Loading swing out rotors Loading of the buckets vessels must be made in accordance to figure 15 It is allowed to operate e g a 4 place rotor with 2 loaded buckets only But the loaded buckets must be opposite to each other Make sure that the unloaded buckets also be put inside the rotor see figure 14 and 15 In principle swing out rotors may not be taken in operation until all buckets or racks are put into the rotor The bolts at the rotor must be greased with the HERMLE High EF oil Order No 34 5147 The sample tubes have to be filled evenly by eye and put into the drillings or tube racks The weight difference of the loaded buckets should not exceed approx 1 0 g A ATTENTION Swing out rotors may be taken in operation only if all locations are filled in with either four buckets or four carriers do not mix buckets and carriers up Figure 14 wrong Figure 15 correct A ATTENTION Do not operate the centrifuge with rotors or buckets which show any signs of corrosion or mechanical damage Do not operate with extremely corrosive substances which could damage the rotor and buckets In case of any questions please contact the manufacturer O Hermle Labortechnik GmbH Z306 Instruction manual docx 13 2 1 4 Loa
35. tors and accessories might be used Any other use or intended use is considered improper From the resulting damage the company Hermle Labortechnik is not liable 1 2 Brief description The unit type Z 306 is a non refrigerated universal centrifuge which we offer in two voltage variations 230V or 120V The centrifuge can be used with swing out and angle rotors All parameters are accessible via buttons and selected with the central adjuster All pre selected and current values will be shown permanently on the LCD display The centrifuge is powered by a maintenance free induction motor Detailed technical data are in table 1 Technical data see APPENDIX P V 1 3 Delivery package e 1 Centrifuge Z 306 e 1 Operating Manual Z 306 e 1 Rotor key Rotor s Accessories will be packed separate Hermle Labortechnik GmbH Z306 Instruction manual docx 1 4 Operating and display elements OO N O A A O N ck N O central adjuster run parameters 0 1 LCD rpm rcf accel decel time lid quick start stop prog store power switch control panel display speed g force acceleration Deceleration intensity centrifugation time lid release short running start centrifugation stop centrifugation calling stored programs program store Z306 Instruction manual docx O Hermle Labortechnik GmbH 1 4 1 LCD Display Figure 1 Display fields A 1 A 2 A 3 Display field rom rcf
36. transport packaging 25 bis 55 C 10 bis 75 70 bis 106 kPa 6 3 Disposal In the event of disposing of the product please observe the applicable legal regulations Information on the disposal of the electrical and electronic devices in the European Community The disposal of the electrical devices is regulated within the European Community by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE In accordance with this any devices delivered after 13 08 2005 on a business to business basis which includes the product may no longer be disposed of in household waste To document this they have been marked with the following identification ZS Because disposal regulations may differ from one country to another within the EU please contact your supplier if necessary 30 Z306 Instruction manual docx O Hermle Labortechnik GmbH mome 7 APPENDIX EC Conformity Declaration a nannenneneneenennenenenennenennenennenenneneenenennenennenenne IV Table 1 Technical DATA a ios V Table 2 Permissible net weight 2 22 22 222202222020220020000000202200nan2unnununnnnnnnnnnnnnnnnnnnnnnnn0 VI Table 3 Max speed and RCF values for permissible rotors een VII Table 4 Acceleration and deceleration times ss VIII Table b Error MESSAGES inne een ee IX TABS 6 Radius GOGON sawau inaa ai anaa iang a Degan ka aa bahane abiane anana ka baia X Table 7 Abbreviation
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