Q-View User Manual
Contents
1. Gamma t Image Quansys Biosciences Toll Free 888 Q uansys 782 6797 i 365 North 600 West Fax 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com Image 0596 CR2 Quansys Q View Software EA imag E File Image Scaling Fiters Tools Multiplex ELISA Analysis Help cg EEx xaucGG 5 76e6G0626G9 2 Ready a VU ag Start Untitled 1 uw X Gamma Image Quansys Biosciences Toll Free 888 Q uansys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com x Image 0596 CR2 Quansys Q View Software cif JE File Image Scaling Fiters Tools Multiplex ELISA Analysis Help CA Mx x AaG G ES i Ready s Mc nm EL NUM E Brightness Image Quansys Biosciences Toll Free 888 Q ua nsys 782 6797 365 North 600 West Fax 435 750 6869 Better Testing Better Results Logan Utah 84321 info quansysbio com El Image_0596 CR2 Quansys Q View Software zl File Image Scaling Fiters Tools Multiplex ELISA Analysis Help om Mxxaacec oo eee Decrease Brightness Decrease Brightness NUM ES i 73 start x E image 0596 R2 Qu TERI Brightness Image Quansys Biosciences Toll Free 888 Q ua nsys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com sf Image 0596 CR2 Quansys Q View Sofi p software led File Image Scalin2. speed and image file size The Open Grid Layout enables the userto open previously saved grids for facilitating spot analysis The Save Grid Layout feature allowsthe userto scan multiple imagesthat share the same layout The gridslayout can be opened on a new image and analyzed The save grid layout is discussed in greater detail in the image analysis section Continue to Image Scaling Menu Image Scaling Menu The Image Scaling menu is used to scale the image to any size once the image has been loaded It can be used to view one enlarged section of the imge orto scale the ientire image down so it can be viewed entirely on one canvas View image example Image Scaling Zoom Page adjusts the image to fit into one page The Zoom In command increased the image size on the monitor while the Zoom Out command decreases the image size on the monitor Continue to Filters Menu Filters Menu The Filters Menu contains features that enhance the userss ability to enhance the image to make viewing responses easier While these tools visibly alter what is observed on the monitor the data output isindependent of Gamma or Brightness settings and will be unaltered View image example Filters Menu Gamma t Gamma Brightness Brightness Continue to Tools Menu Gamma Gamma Gamma Increase Increasing the gamma allows you to make the image brighter In the case of microamay images it lets you look at the wells and spots
3. which are dull and not visible when the image is first loaded View image example Gamma Gamma Gamma Decrease Thisactsasthe opposit of the gamma plusand decreases the gamma of the image bya certain pre fixed amount Since itsthe exact opposite of Gamma one application of the gamma plus filter can be offset by one application of the Gamma filter and vice versa View image example Gamma Brightness Brightness Increase Brightness Increasing the brightness of the image increases the values of all pixels in the image by the same uniform scaling factor This makes darker images easier to view View image example Brightness Brightness Brightness Decrease This makes the images darker by reducing all the pixels in the iamge by the same uniform scaling amount Since this is the exact opposit of the Brightness feature one application of the Brghtness can be cancelled out by one application of the Brightness and vice versa View image example Brightness Tools Menu The Tools Menu contains options used for assay analysis The Ellipse Tool enablesthe userto select spots for analysis The size of this ellipse can be altered by using the increase or decrease spot size commands After clic king the ellipse command in the tools menu the cursor should change from an amow to an ellipse Place the cursoroverthe spots desired for analysis and click the left mouse button The spot is selected for subsequent analysis View im
4. 6 CR2 Quansys Q View Sofi p software led File Image Scaling Fiters Tools Multiplex ELISA Analysis Help ama MxxARG G s oOo BOSE Ready Duplicated Grid Imag Quansys Biosciences Toll Free 888 Q uansys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com L Image 0596 CR2 Quansys Q View Software fe EI File Image Scaling Filters Tools Multiplex ELISA Analysis Help MaMxxAaneoc e soo 68060 Ready Drag Grid Image Quansys Biosciences Toll Free 888 Q uansys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com Image 0596 CR2 Quansys Q View Software g J ICI Image Scaling Filters Tools Multiplex ELISA Analysis Help eerie Open Ctrl 0o GG 660 2 Open Grid Layout Save Grid Layout Ext Save Grid Image Quansys Biosciences Toll Free 888 Q ua nsys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com d Image 0596 CR2 Qi uansy File Image Scaling Filters Tools Sma mxx aac Je jx Help EF ERR TZE eeo Duplicate Grids Delete Grids Delete All Labels Analyze Spots titled Quan ICTV Multiplex ELISA Analysis Menu Image Quansys Biosciences Toll Free 888 Q ua nsys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing B
5. Quansy Q View Software Help Table of Contents QUANSYS BIOSCIE Get Started Now Abbreviated Software Protocol Analyze Spots Brightness Create a Grid File Menu Filters Menu Gamma Getting Started Image Scaling Menu Multiplex ELISA Menu ToolsMenu Getting Started Open an image file by selecting Open in the file menu Compatible formats Tiff peg CR2 CR2 imagesare the best to work with as they have the best resolution possible with the Canon Camera Tiff images are recommended over peg images because they are not compressed Adjust the image until the spots are easily visible Zoom in on the image until the spots are easily visible by pressing the zoom in icon magnifying glass with a plus sign Tum the gamma up if the spots are dim by clicking on the icon labeled with a G Hup orG down Select the ellipse orthe rectangle icon on the Editing toolbar Draw an ellipse around each spot Be sure to draw the objectsin the order you want the data exported It is best to draw the object slightly smaller then the spots Do not let the object overlap another spot Analyzing the spots Continue to File Menu File Menu The File Menu can be used to open image files save grid layouts and open grid layouts View image example File Menu When Opening Image Filesa dialog box wil appearindicating the progress made in opening the file The time to open an image isdependent upon computer
6. The Analyze Spots command directs the software to analyze the spotsin the area circled by the user The Duplicate Grid copiesand pastesa replicate of the selected ellipses The Delete Grid allows the user to delete all selected red ellipse The Delete All Labels allows the userto deleted the labels placed onto each spot by the software flowing analysis so the usercan match the Spot ID with the spot on the image View image example Delete Grid Labels Continue to Analyze Spots Analyze Spots The Analyze Spots command directs the software to analyze the spotsin the areascircled by the user View image example Analyze Spots The software will then analyze the selected spots for pixel intensity in the order that the ellipses were drawn View image example Select Spot Analysis Data can be pasted into a spreadsheet for further organization and analysis Select one cell with the spot value press Select All Ctrl A then Copy Ctrl4C and Paste Ctri2V into your spreadsheet View image example Copy All Results Continue to Abbreviated Software Protocol Abbreviated Software Protocol Load the image desired for analysis Size the image asdesired Click the Ellipse Tool icon Encircle the desired spot with the icon and click to mark for analysis Repeat for all desired spots Click the Analyze Spots icon Copy and paste the data into favored spreadsheet Quansys Biosciences Toll Free 888 Q ua n
7. age example Selected Spot The Rectangle Tool is provided forthe analysis of Westem immunoblots Continue to Create a Grid Create a Grid After clicking the Ellipse Command in the tools menu the cursor should change from an amow to an ellipse Place the cursor over the spots desired for analysis and click the left mouse button The spot is selected for subsequent analysis View image example Create Spot After selecting the desired number of spotsin a well click on the Select Tool Icon in the toolbarand highlight the selected spots The selected spots will tum red once highlighted View image example Select Spots These spotscan be copied by pressing the letter P on the keyboard orthe Duplicate Grids command in the Multiplex ELISA Analysis menu View image example Duplicate Grid The new grids will be pasted onto the image and will be pasted ready for manipulation in red View image example Duplicate Grid Press and hold the shift key which commandsthe software to highlight all selected ellipses Drag the Grid onto a new well View image example Drag Grid By clicking on the Save Grid La yout in the file menu the series of ellipses can be saved foruse on otherimages View image example Sa ve Grid Continue to Multiplex EUSA Analysis Menu Multiplex ELISA Analysis Menu The Multiplex ELISA Analysis Menu contains features for spot analysis View image example Multiplex ELISA Analysis Menu
8. c oSe eeco 2 NIN IN IN IN IR INIR 3 9 N S a om joo oo 218 2 3 Ready NUM Copy All Results Image Quansys Biosciences Toll Free 888 Quansys 782 6797 365 North 600 West Fax 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com
9. etter Results Logan Utah 84321 info quansysbio com Image _0596 CR2 Quansy I File Image Scaling Fiters Tools MUTTSETTUNCAN Help amp E u x x A A C Analyze Spots E C060 Duplicate Grids Delete Grids Delete All Labels Deleted Grid Labels Image Quansys Biosciences Toll Free 888 Q uansys 782 6797 365 North 600 West Fax 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com cmEExxaacc IxBB53leeo Spot Value 56880 12748 16018 10611 6636 5509 42162 44405 58109 20283 20617 34970 15973 49917 57207 17490 13076 15589 10840 5861 302 Ready Analyze Spots Image NUM Quansys Biosciences Toll Free 888 Q uansys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com cmxmHxx xaacc e i553eeoeo Spot Value 56880 19339 12748 16018 10811 a lo n 42162 44405 58109 o lo zs 20283 20617 DI 34970 15973 49917 57207 17490 13076 15589 m 10840 5861 59882 6376 42083 44474 58276 18789 19547 31964 15789 4044 n 12 13 14 15 16 17 18 19 20 1 2 3 m NIN N a Ready NUM Select Spot Analysis Image Quansys Biosciences Toll Free 888 Q uansys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com SHiMxx SAAac G
10. g Fiters Tools Multiplex ELISA Analysis Help mn aa MxxARG G s Go BOO Ready Selected Spot Image Quansys Biosciences Toll Free 888 Q uansys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com sf Image 0596 CR2 Quansys Q View Sofi software File Image Scaling Fiters Tools Multiplex ELISA Analysis Help M Mx x Aaneac e cs OG 6 eq Ready Create Spot Image Better Testing Better Results Quansys Biosciences 365 North 600 West Logan Utah 84321 Toll Free 888 Q uansys 782 6797 Fax 435 750 6869 info quansysbio com sf Image 0596 CR2 Quansys Q View Sofi J software File Image Scaling Fiters Tools Multiplex ELISA Analysis Help cEXX XA amp AGG 4 Ready Select Spot Image eog Better Testing Better Results Quansys Biosciences 365 North 600 West Logan Utah 84321 Toll Free 888 Q ua nsys 782 6797 Fax 435 750 6869 info quansysbio com sd Image 0596 CR2 Quansys Q View Software lt File Image Scaling Fiters Tools ultiplex EL Help MIXX AG C_ Analyze EL HOS 9 Delete Grids Delete All Labels Duplicate Selected Grids NUM ad Start ly Untitled Quan a EN Duplicate Grid Image Quansys Biosciences Toll Free 888 Q uansys 782 6797 365 North 600 West Fa x 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com sf Image 059
11. sys 782 6797 365 North 600 West Fax 435 750 6869 Better Testing Better Results Logan Utah 84321 info quansysbio com El Image 0596 CR2 Quansys Q View Software Ne l3 Image Scaling Filters Tools Multiplex ELISA Analysis Help Open Ctr o IGG gt Sa eoe Open Grid Layout Save Grid Layout Er File Menu Image Quansys Biosciences Toll Free 888 Q ua nsys 782 6797 365 North 600 West Fax 435 750 6869 Better Testing Better Results Logan Utah 84321 info qua nsysbio com Image _0596 CR2 Quansys Q View Softw File Bite nem Filters Tools Multiplex ELISA Analysis Help gt Zoom Page Ia a GG X eoe Zoom In Zoom Out Image Scaling Image Better Testing Better Results Quansys Biosciences 365 North 600 West Logan Utah 84321 Toll Free 888 Q ua nsys 782 6797 Fax 435 750 6869 info quansysbio com Image 0596 CR2 Quansys Q View Softw File Image Scaling OEE Tools Multiplex ELISA Analysis Help um fa ul x Gamma f iR S eo oe Gamma Brightness Brightness Filter Menu Image Better Testing Better Results Quansys Biosciences 365 North 600 West Logan Utah 84321 Toll Free 888 Q uansys 782 6797 Fax 435 750 6869 info quansysbio com Ed Image 0596 CR2 Quansys 0 View Software gt lt File Image Scaling Filters Tools Multiplex ELISA Analysis Help MaMxxAaneGo e soo eeq Increase Gamma Increase Gamma NUM
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