USER`S MANUAL - Madell Technology Corporation
Contents
1. 7 Detection modes single wavelength or dual wavelengths 8 Display 320 x 240 pixels LCD screen 9 Print external printer 10 Communication RS232 serial port 11 Output absorbency cut off value qualitative judgment value of P CO concentration judgment of normal value and quality control value 3 3 Power Supply 1 Voltage 220X 1 10 V 2 Frequency 50x 1 2 Hz 3 Power supply must be earthed perfectly 4 PANEL 4 1 Back Panel lt 3 eS eT Switch Fuse Power RS232 Port Print nort Fan Fig 3 Back Panel 4 2 Preparation and Examination You should first examine the instrument whether there are scathes such as distortion and crack for the sake of inappropriate transportation and storage If there isn t any scathe you can insert into the electric socket and switch on the instrument The instrument will auto check at first If there is something wrong the corresponding prompt will display on the screen For example if a filter fails the instrument displays Filter failure if the optical system has malfunction it displays Optical system failure You should correct these mistakes before using the instrument If the auto checking passes the machine will go to the main menu and wait for orders At that time you can operate program detect inquire according to your demands 5 Before you begin to detect you must wait 20 minutes for a complete warm up since the instrument has be2. DNM 9602G Microplate Reader USER S MANUAL Madell Technology Corp 7372 Walnut Ave Suite V Buena Park CA 90620 USA Phone 1 877 670 9023 http www madellitech com CONTENTS 1 INTRODUCTION 1 1 CHARACTERS 1 2 MAIN FUNCTIONS 1 3 DENOMINATING RULE 1 4 WORK CONDITIONS 1 5 DIMENSIONS AND WEIGHT 2 PRINCIPLE AND FEATURES 2 1 WORK PRINCIPLE 2 2 STRUCTURE FEATURES 2 3 SYSTEM CONFIGURATION 3 SPECIFICATIONS 3 1 FUNCTIONS 3 2 TECHINCAL SPECIFICATIONS 3 3 POWER SUPPLY 4 PANEL 4 1 BACK PANEL 4 2 PREPARATIONS AND EXAMINATION 5 PROGRAMMING 5 1 PROGRAMMING INTRODUCTION 5 2 PROGRAMMING OPERATION 6 DETECTION 7 SYSTEM SETTING 7 1 TIME SETTING 7 2 WAVELENGTH SETTING 8 INQUIRY 9 MAINTENANCE 9 1 FILTER 9 2 LAMP 9 3 TRAY 9 4 FUSE 10 TRANSPORTATION 11 PACKING LIST 12 QUALITY GUARANTEE 13 WARNING 1 INTRODUCTION 1 1 Characters The DNM 9602G Microplate Reader is a semi automated bench top instrument focused on clinical immunity test in hospitals or epidemic preventing stations It has good characters such as high sensitivity high accuracy high speed of detection and the easiness of operation etc Display is a liquid crystal one with alphanumeric and graphic options Parameters can be checked on the display without using difficult command sequences 1 2 Main Functions 1 The instrument is mostly used for the detections of viruses germina symbols of tumor human body s hormones and other items based on the princ
3. inputted value see the function of SET 4 Select single well detection or dual wells detection If you select the latter the instrument will combine two wells in the row direction as A1 A2 A3 A4 H11 H12 and use the mean of the two absorbencies 5 If you want to exit programming click the up arrow in the status bar in the right side of the screen or click the icon at the left bottom Click the down arrow to go to the next interface of programming 5 2 2 Vibration Mode and Detection Mode Explanation 1 The instrument has the function of vibration But we suggest you use external vibration if you have 2 If you only want to get the absorbency of the sample and do not need any analysis and calculation please select Absorbency 3 When you need to get the quality for example positive or negative of the sample please select Qualitative Detection 4 When you need to get the concentration of the sample please select Quantitative Detection 5 Click the icon at the left bottom to exit programming click the up arrow in the status bar to return to the first interface of programming and click the down arrow in the status bar to go into the next interface 5 2 3 Set Blank Well The instrument allows randomly setting blank wells and mosily set 8 blank wells at one time User can select the mode of Average Column Blank Wells and set all the 8 wells of one column as blank
4. item parameters in the instrument and then return to the main menu If you click Exit the instrument will not save the parameters Click the up arrow to return to the previous interface 5 2 6 Absorbency Detection Mode Click Absorbency in the interface of Detection Mode and then click the down arrow in the status bar to enter the interface of blank wells setting After you set the blank wells click the down arrow in the status bar Then save the results The operations are the same as above 5 2 7 Kinetic Detection Mode 5 2 7 1 Click Kinetic Detection in the interface of Detection Mode and then click the down arrow in the status bar to enter the following interface Explanation 1 Click underline to input the corresponding parameters Unit of delay time and detection time is second The instrument adjusts interval between two detections based on the detection time input The sampling times is 6 seconds in the detection period 2 Calculating formula Activity of the enzyme A A minx F D IU L In above formula F means factor 6 DETECTION The instrument can be used to clinical test after programming Caution To ensure veracity of the detecting results you must arrange the places of the blank wells negative control wells and the positive control wells on the board to be consistent with the detection program 6 1 Switch the instrument on and then it will start self checking first If self checking passe
5. with the arrangement of the filters in the tray otherwise it will lead to wrong results There are four filters in the instrument and the turn of them is 405nm 450nm 492nm and 630nm 7 2 2 The instrument can set 7 filters at most If the number of wavelength is less than 7 input O 7 2 3 Continue clicking Delete all items and then the numbers of 1 2 3 appear in turn When the number 3 appears the instrument will save all items information and the wavelengths set But all the detection results will be deleted 7 2 4 The function of Debug is used for checking work parameters when the instrument is maintaining Click the icon of STOP to exit the function 8 INQUIRY The function is designed for inquiring the saved quality control data and detection results 8 1 Inquiry Introduction Click Inquire in the main menu and then the following interface appears For example Explanation 1 If you need to print the quality control results click Result of QC The screen displays the symbol of The symbol means the item number 0 5 The six items are quality control items detected in this month When you input the item number the LCD shows the data of this item 2 When Detection Result is selected the screen will display the list of detection results Enter the result number you want to inquire Then the display shows the saved result There are 30 results saved in the instrument Click the up arrow or
6. d to be transmitted to the external PC please connect the signal wire first and make the PC in the state of waiting Please see the user s manual of Software of DNM 9602G irmans Microplate Reader When the screen shows the absorbency click then the picture of Ei on the screen It means the data are transmitting now Until the transmitting is finished the picture of does not disappear 6 6 When all the work above is finished take the enzymatic board out If you still want to continue detection of the item replace a new enzymatic board and repeat the operating steps since step 6 4 If you wish to detect other items please repeat operating steps since step 6 1 6 7 After all the detection items are finished return to the main menu Click the menu of Finish the tray of the enzymatic board will return to the instrument automatically if you click Finish again the tray of the enzymatic board will come out Then you can shut off the power and pull out the plug 6 8 When the detection method is kinetic method the display will show the A T reaction chart after detection Click the 15 icon at the bottom to switch the sample from 1 to 8 6 9 When the calculation mode is Regression Technique Semi log Technique Total Logarithmic Technique or Ratio Logarithmic Technique of quantitative detection and select the item of Use results of this detection the screen displays the standard curve after detection For examp
7. en switched on If the instrument has no operations for 30 minutes it will automatically switch to the sleeping state in order to prolong its life When you start operating again the instrument will return to the normal state 5 PROGRAMMING Before beginning detection you should first program according to your demands In order for convenience of later work order of program numbers should be based on the following principles 1 Put the most commonly used detection items in the front and arrange combined items together 2 Because the instrument only allows five items to process quality controls synchronously you should put the item which needs quality control in the first five items when programming 5 1 Programming Introduction 5 1 1 The following picture is the main menu of the instrument Explanation Detect After the programming is finished you can click the icon to begin to detect It allows to select item set the row number and the column number and decide whether to use the calibrating factors saved in the instrument or not Program Click the icon to enter the interface of programming The instrument can save 120 programs from O to 119 Set The instrument allows setting wavelengths and the system time and deleting all items saved detection results quality control results and the calibrating factors Inquire Query the result of quality control detection results and parameters of programs Time After you click
8. en the instrument displays the next interface of inputting the parameters A B D Please input the three parameters according to the reagent specification Click the down arrow in the status bar to finish the operation of inputting the three parameters The parameters set will not display in the J33 Ll 7J process of programming but appear in detection If the calculation mode is Single means positive and means negative 3 The Dual calculation mode demands to input two groups of parameters The first group is A1 B1 and D1 The second group is A2 B2 and D2 Take these two cut off values as upper limit and lower limit to judge quality positive or negative Inputting method is the same as that of Single If the calculation mode is Dual means positive means negative blank means the absorbency is between the two cut off values 4 Please input two parameters at the position of Limit Value Click the underline and the keyboard appears The two parameters are the upper limit and the lower limit for subsection of absorbencies Make the difference of the two parameters to 10 average shares The difference between the two absorbencies and the lower limit is put to the corresponding subsection The result is a number from 0 to 9 For example input two parameters 0 5 and 1 0 Average subsection is 0 05 If the absorbency is 0 6 the difference between the sample s absorbency and the
9. iples of immunology in the clinical tests 2 Corresponding detection for microanalysis in chemistry or biochemistry 1 3 Denominating Rule DN M 9602 G Lo Serial Number Designing Number Symbol of the instrument Symbol of computer 1 4 Work Conditions 1 Environmental temperature 10 30 C 2 Relative humidity lt 0 3 Keep away from strongly electromagnetic field and avoid direct irradiation by strong light 4 Avoid dust and shake There must be no caustic gas such as hydrochloric acid in air 5 Before you begin to detect you must wait 20 minutes for a complete warm up since the instrument has been switched on 1 5 Dimensions and Weight Dimension 420 x 325x 210 mm Ix hx w Weight 10 kg 2 PRINCIPLE AND FEATURES 2 1 Work Principle Work principle of the instrument is photoelectric colorimetry Firstly the instrument detects absorbencies of the under detecting sample and the standard liquid Secondly it analyses and calculates the absorbencies Finally it produces the value or the condition of the under detecting material in the sample liquid 2 2 Structure Features The instrument has two main components the mainframe and the printer The mainframe is composed of optic system 8 channel detection system single chip system and enzymatic plate driving system The instrument can detect enzymatic plate that has 96 or 48 wells 2 3 System Configuration Optic 8 channel Enzymatic Photoelectric am F system Prisma
10. le The Y coordinate may be absorbency A or logig A and the abscissa may be concentration C or 0919 C which is decided by the calculation mode selected The line is confirmed by the calibrating factor The letter r denotes the lineal correlating ratio and the pink box denotes the detection value of the standard liquid You can click the icon Edit to change the absorbencies of the standard liquids For example Click the corresponding position of the number and input a new absorbency When the new number has been entered click the up or down arrow in the status bar and return to the interface where calibrating curve shows Then calculate the correlating ratio again If you click Next it will calculate the sample s concentration using the calibrating factor displayed in this interface The calibrating factor a and b can be showed and changed at the beginning of detection 7 SYSTEM SETTING Click Set in the main menu and then the following interface appears For example 7 1 Time Setting When there is something wrong with the date or the time displayed in the screen you can select the function and input the right date and time in turn in the next menu First click the position second using the keyboard at the bottom to input The bit you input is decided by the symbol of 7 2 Wavelength Setting 7 2 1 Input the wavelengths nm of the filters in turn Pay attention to make the input data accord
11. lower limit is 0 1 and then the result is 2 5 If the regent specification prescribes that when the negative control value is less than the input value for example 0 05 you should calculate according to the input value please click the corresponding underline and input the regulated value If the regent specification prescribes that when the negative control value is more than the input value for example 1 20 you should calculate according to the input value please click the corresponding underline and input the regulated value 10 6 Click the little icon at the left bottom to exit programming Click the up arrow in the status bar to return to the interface of blank wells setting Click the down arrow in the status bar to go to the next interface 5 2 4 2 Invalidity Judgment and Wells Setting Explanation 1 Reagent specifications of some detection items have prescribed the validity judgment for the absorbencies of positive control samples and negative control samples to ensure validity of detection results On this circumstance please input the prescriptive value as the reagent specification demand If the reagent specification has no demands do not input and the instrument doesn t do the validity judgment when detecting 2 The quality control well must be one When you input negative or positive wells please input the well s number first and then input the position 3 If there is no change about the inputted items and you wi
12. ment it will appear a letter after first clicking and will appear the corresponding number after second clicking 5 1 3 There is a status bar on the left side of the display when you are programming Click the up arrow to return to the previous menu and click the down arrow to go to the next menu 5 1 4 You can freely set blank negative positive and quality control wells on the enzymatic plate when you are doing qualitative detection And you can set blank negative and positive wells from 1 to 3 respectively But the quality control well must be no more than one When you are doing quantitative detection you can set standard wells from small to large values according to rows or columns 5 1 5 In the process of programming you can click the icon on the left bottom to exit programming and return to the main menu 5 2 Programming Operation 5 2 1 Programming Click the icon of Program in the main menu The next display will show for example Explanation 1 After you click Item No the keyboard will appear Then you can enter the item No 0 119 2 Click Item Name to input the name of the program The name may be a combination of numbers and letters for example 8IG If you have not entered a name the system will designate a default one 3 Select a working mode of single wavelength or dual wavelengths based on the reagent specification The pink box means the current selection Click the number of wavelength to change the
13. needs to input the number of the standard wells and the instrument will automatically set wells positions based on the direction of the standard wells and detection demands The instrument will show the positions of the standard wells when it displays the item information before detection Caution You must first set the direction of the standard wells and then input the quality control well If the direction of the standard wells has been changed the quality control well must be set again 3 The direction A1 B1 of the standard wells means put the standard wells according to the column direction and A1 A2 means row direction 4 Click the icon at the left bottom to exit programming Click the up arrow in the status bar to return to the previous interface Click the down arrow in the status bar to go to the next interface 5 2 5 3 Set Standard Values 12 Explanation 1 Click Concentration to input the standard concentrations as the reagent specification demands in the order from small values to large values Please input two concentrations each line Use the keyboard to ERTEH to finish this inputting Caution When the detection method is logarithm method you can t input the first concentration input values and then press value if it is 0 otherwise the detection results will be wrong 2 If you need to do judge the normal values click Normal Value and then input two normal values 3 Click Save to keep the
14. o click the down arrow to begin detection 6 4 When the detection is started the screen displays Detecting now please wait After a few seconds the detection is finished First the instrument displays absorbency of every well second it give the result of qualitative or quantitative detection Results from column 1 to column 6 first show on LCD If you wish to see the results from column 7 to column 12 please click the button of Next at the bottom of the display The symbol of means there is no detection in the position If you want to print the absorbency please click 14 Fem Click pa SAVE click Ene The instrument sends all the data to the printer together You can do other operations once the to go into the next interface or return If the detecting results need to be stored for query printing starts and needn t wait The printing format shows as table 3 Item Plate Wavelength Date Time Operator A B C D E F G H Negative Positive Cut off value Quality control Explanation The program decides the output of detecting results Detecting results are mainly four 1 Absorbency 2 Result of cut off judgment and value of P CO 3 Concentration 4 Judgment based on normal concentration value H L If the detecting result of concentration is 99999 it means the concentration of the sample is beyond the linear range You should dilute it and detect again 6 5 When the detection results nee
15. r you have disassembled the instrument without authorization We will answer maintenance related questions for the instrument even after the one year warranty period 13 WARNING 1 The power supply should be grounded perfectly 2 Filters must be changed by a trained professional Madell Technology Corp 7372 Walnut Ave Suite V Buena Park CA 90620 USA Phone 1 877 670 9023 http www madelitech com 19
16. s it will display 13 the main menu At that time you should not detect immediately Please wait 20 minutes for a complete warm up before you begin to detect 6 2 Click Detect in the main menu and then the following detection interface appears For example Humber Hame Humber The LCD shows item names and item numbers of 18 programs at one time Click the down arrow in the status bar to see parameters of other programs Please select a program number before detection After selection click the icon to return to the main menu If the direction of detection is A1 A2 click the string bar Detecting to Col __ and then change the column number if the direction of detection is A1 B1 click the string bar Row___ and then change the row number The instrument has saved calibrating factors of quantitative detection and the cut off values CO1 CO2 of qualitative detection You can use these saved parameters to calculate click the corresponding parameter to change or select Use result of this detection If you select the latter please input standard liquids to calibrate again and use the new results to calculate If you select the former the sample can be put on the standard wells to detect 6 3 Click the up arrow in the status bar to return to the previous interface or click the down arrow to show information of the detection item selected When the correct information has been displayed continue t
17. sh to use them when detecting please click the icon of Save Click the icon of Exit to quit this programming Click the up arrow in the status bar to return to the superiors interface Thus the programming of qualitative detection mode is complete 5 2 5 Quantitative Detection Mode 5 2 5 1 Detection Mode Click Quantitative Detection in the interface of Detection Mode and then click the down arrow in the status bar to enter the interface of selecting calculation method Explanation 1 There are two standard values in the linear technique Directly calculate the sample s concentration according to the linear proportion 2 The method of interpolation technique uses the sections of the standard liquids concentrations to 11 calculate the sample s concentration 3 Click the icon at the left bottom to exit programming Click the up arrow in the status bar to return to the previous interface Click the down arrow in the status bar to go to the next interface 5 2 5 2 Set Wells Explanation 1 Click the underline after Dilution Factor to input the real dilution multiple When the detection mode is Ratio Logarithmic Technique the factor input is used for calculation coefficient revised factor 2 When the detection mode is concentration detection the position of the blank well is fixed There is one quality control well You should select whether to have quality control data or not You only
18. strument and examine it If you confirm that the lamp is invalid please change for the same standard lamp lf something is wrong with the power you should repair it 9 3 Tray When there is something wrong with the tray it can t pass in and out of the instrument there are some possible reasons down lead of the step motor does not perfectly link synchronous strap is loose or doffed movement parts shift and block etc Please find out the causation and get rid of the malfunction 9 4 Fuse lf the fuse burns out you must cut off the power supply at first and then change the fuse for the same standard You may as well change the fuse under the professional s guidance 18 10 TRANSPORTATION 1 Range of environmental temperature 20 C 55 C 2 Relative humidity of environment lt 93 3 Range of atmosphere pressure 500hpa 1060hpa 11 PACKING LIST Name Number 1 DNM 9602G Microplate Reader 1 2 Printer 1 3 User s manual 1 4 Product certificate 1 5 User checking and accepting sheet 1 6 Accessories a Wire for power supply 1 b Fuse 5x 20 2A 2 c Plastic cover 1 12 QUALITY GUARANTEE We will repair or replace the instrument or accessories free of charge if there is any problem with the instrument provided that you have complied with the instructions of this operating manual and it is within one year from the date of purchase You will be charged if you have not followed this operating manual o
19. the down arrow to go other interface Click the icon of STOP to exit inquiry 3 When the instrument displays the detection results or the quality control results a keyboard appears at se save fons When inquiring the detection results the user should select Print or Trans and the function of Save the bottom is invalid But when the instrument displays the quality control results the functions of Trans and Save are invalid and the function of Next is changing between quality control results and quality control chart 4 The format of Item Information is the same as that appeared in the interface of detection Click Item OKKKKI Information and the display shows At this moment enter the item number 1 3 bits and click EHTE 5 Click the up or down arrow in the status bar to leave the interface 9 MAINTENANCE The instrument only needs cleaning in normal usage But the following assemblies will be invalid or have malfunctions after a long time usage You may as well maintain them under the guidance of professional 9 1 Filter The transmission rate of filters will be declined after a period of usage When the instrument doesn t work naturally it will give you a clue In order for accuracy of detection the invalid filter must be changed by professional in time 9 2 Lamp The instrument will prompt you if the light lamp fails At that time you should open the lid of the in
20. the icon of Time the current time shows on the bottom of the display The time will show a few seconds and then return to the main menu Finish After you click the icon of Finish the tray returns to the instrument and then the instrument does not respond for other icons Till you click the icon again to make the tray comes out you can click other functional icons Caution After you click the icon of Finish the system will save some important parameters For example results of quality control and detection results So you must click the icon of 6 Finish and wait the tray returning to the instrument Then turn off the instrument 5 1 2 When you need to input some numbers click the inputting position Then a keyboard will be fo You can directly click the number you wan to select When all the numbers have been entered displayed as EHTE DEL press If the number needs to be changed please click the button to delete the number entered and enter again The item name may be a combination of numbers and letters After you click the inputting position of the item name a keyboard will be displayed as oe A a 5 T B g DEL 1 4 In above keyboard 1 A means a conversion between number and letter When it is conversed to WEIT letter the button will appear for selecting letters from A to Z When the well s position is needed to select the keyboard will be displayed as TAEA ef eave ef 9 ol e In this mo
21. tic organ plate detection Steady voltage CPU Simulative signal disposal power and A D conversion circuit Display Keyboard RS232 serial port Fig 1 System Configuration 3 SPECIFICATIONS 3 1 Functions 1 The instrument can detect absorbency and do quantitative qualitative and kinetic detection 2 The instrument maximally memories 120 programs in common use and displays parameters of the programs on LCD 3 The instrument can automatically select filters for different items 4 When the instrument starts up it will auto check at first Then an initial setting and self check will begin to operate If there is something wrong the corresponding prompt will appear on the screen 5 The instrument has printing function 3 2 Technical Specifications 1 Range of working wavelength is from 400nm to 700nm It has 4 filters when leaving factory 405nm 450nm 492nm 6380nm 2 Characters of the filters Accuracy of wave nm Half width nm 3 0 lt 12 Table 1 Characters of the Filters 3 Detection range of the instrument is from 0 1A to 3 0A The errors of absorbency accuracy are as Riorana A 0 1 1 0 1 0 or 0 01A follows gt 1 0 2 0 2 0 or 0 02A gt 2 0 3 0 5 0 or 0 05A L 3 0 4 0 8 0 or 0 15A Table 2 Absorbency Accuracy 4 Absorbency A Error Table 3 Linearity of the Instrument 5 Reproducibility Error lt 0 5 6 Stability Error lt 0 005A 1 Omin
22. wells For example Explanation 1 When you select Randomly Set click the strings and input the number of blank wells Then input the positions of the blank wells When you select Average Column Blank Wells directly input the column number and needn t input position of every well 2 Click the icon at the left bottom to exit programming Click the up arrow in the status bar to return to the interface of Vibration mode and detection mode Click the down arrow in the status bar to go to the next interface Caution When the detection mode is quantitative detection or Kinetic Detection positions of blank wells are fixed and do not allow to set 5 2 4 Qualitative Detection Mode 5 2 4 1 Calculation Mode Select the qualitative detection mode in the interface of Detection Mode and enter the interface of Blank Well Click the down arrow in the interface to enter the qualitative detection interface to set calculation mode qualitative mode and negative comparison value For example Explanation 1 The definitions of letters in above formula are as follows NCV Negative comparison value P Absorbency of under detection sample N Average absorbency of negative control wells Y Average absorbency of positive control wells A B D A1 B1 D1 A2 B2 D2 Parameters needed to input according to reagent specification CO CO1 CO2 Cut off values used for judgment 2 Click Single and th
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