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1.    Do not operate the unit in premises where condensation can form  Operating conditions of the unit are  defined in the Specifications section     1  Safety precautions Version 2 04     March 2014  Inteliwasher 3D IW8 Page 3    DURING OPERATION    Do not operate the unit in environments with aggressive or explosive chemical mixtures  Please contact  manufacturer for possible operation of the unit in specific atmospheres     Do not operate the unit if itis faulty     Always have the cover protecting against aerosol spread  hereinafter protective cover  installed during  operation     Do not operate the unit if any needle is clogged or malfunctions    Never touch the needles or place fingers or other parts under the needles during operation  Manifold  needles are sharp and can cause injury    Always switch off the unit before changing the manifold    Do not allow the waste bottle to overflow the maximum level during operation  there is a mark on the side    surface of the bottle   Take necessary precautions utilizing waste liquid in accordance with general  laboratory standards     AFTER OPERATION    Carry out the cycle of system washing  on the unit front panel  use the button System rinsing  with  distilled water and do not dry  This will keep the system in permanent operation condition     BIOLOGICAL SAFETY    It is the user s responsibility to carry out appropriate decontamination if hazardous material is spilt on or  penetrates into the equipment   In diagnostic researche
2.  March 2014 4  Operation    Page 12    Inteliwasher 3D IW8    4 19     4 20     When the cycle is completed  a sound signal will inform that the unit stopped the operation  Move to   paragraph 5 of chapter    Operation closedown    ofthis manual    Attention  Always perform the rinsing cycle using distilled water after finishing the operation at the  end of working day  This will help to keep the unit in working order and prevent head  channel clogging    Plate setup    When installing a plate for the first time or when a plate of different type is used  set up the manifold  needles lowering depth     4 20 1  Pressing the Enter key in the start mode and navigate to PLATE SETUP using     and     keys  the    following message appears on the display  fig 9      4 20 2  To confirm calibration  press the Run Stop key        The manifold will measure the depth of the plate well by touching the plate surface first and then the  bottom of the well  Then it will save the difference of the values measured       Shake CLES    Dispense rate  03      Aspirate rate  03      Soak limit s  oo      Shake limit s  30    Fig  9  Manifold lowering depth identification   Dispensed volume   300      Aspirate time  ms  1000      Final aspirate  ms  2000      Wash by rows  YEs      Num of wash cycles  o3      Thechannel  a      Second chan  cycles  on      Secondchannel  oz      Table 3  All parameters of programs with two channels    WASHING PROGRAM    Plate setup  Press RUN button       4  Operat
3.  collecting of waste liquid               o o o   o o    1 piece  Hydrophobic filters for half litre bottle                0 020  ee 2 pieces  Manifold cleaning set    20    ec eee bees 1 piece  Syringe for liquid flushinginhoses         0 0000 cee ete ee 1 piece  POWeENCONd a ao ahs beaded one bh nodes rada eens 1 piece  Operating manual  certificate 2 2    ae 1 copy  4 channel washing solution weight logger  4CHW Logger                  00  on request                   Fig  1  Delivery set    Version 2 04     March 2014 3  Getting started    Page 6    Inteliwasher 3D IW8    3 3      gt  3    3 3 1   3 3 2   3 3 3     3 4 2   3 4 2   3 4 3        Storage and transportation    During long term storage  it is recommended to keep the unit and all its accessories in original packaging  in a dry place protected from dust     Note  Remove the washing solution completely from the hydraulic system in case of long term  storage  more than 8 hrs      Use the original packaging for transportation whether transported by air  water or land    Attention  We do not accept warranty claims related to damages caused by improper packaging   To pack the unit properly    Remove the manifold  tubes and platform for plates    Puton packaging blocks onto sides of the unit and place it into the original carton box     Place the manifold into the protecting case  the syringe  the manifold cleaning set and the platform into  the polythene bag  Place the bag  the case with the manifold in the corners o
4.  side of the rail will move the platform into start position           Fig  2  Installation of platform and manifold    3  Getting started Version 2 04     March 2014  Inteliwasher 3D IW8 Page 7    cr Note  If the plate platform is installed improperly  the magnet will not hold it in place and the  platform will be able to move freely  Move the platform along the rail until the magnets  lock  Increase of resistance to movement indicates that the magnets are locked     3 4 5  Unpack the manifold     3 4 6  Place the manifold on the holder arm so that the guide pins of the manifold slide into the respective  grooves of the holder arm  fig  2 3      3 4 7  Connect the manifold inlet connector supplying the liquid and the Washing Head Dispense  blue   connector at the rear panel of the unit  fig  3 2  with the tube  fig  1 7      3 4 8  Connect the manifold outlet connector and the Washing Head Aspirate     gt   connector at the rear  panel of the unit  fig  3 4  with the tube  fig  1 8      cr Note  Use the provided labels for marking bottles with washing solutions and bottles for  collecting waste liquid and aerosol     3 4 9  Connect the Waste bottle outlet  4     green  on the rear side of the unit to the connector of 2 litre bottle   number 4  for collecting of waste liquid  fig  3 1      3 4 10  Place the tube for the hydrophobic filter  fig  1 9  on the half litre bottle  number 5  connector  then place  the hydrophobic filter on the tube  fig 1 13   When installing the fi
5. 0  eee 8     Numberofwashing CycleS         ooooococc eee ae 1 15  e ASPIFAtON Ume dioramas la Balad ee nb ara db Goan 0 2 3s    Aspiration dispensing speed               a a a a a aa A Ea E a A E a a eee 3 levels    Choice of 3 washing buffers   o DiSpense SYSTEM  raea ar A aa wale x sphincter valve     AA NN 0 300s  incrementis 10s      Number of washed rOWS ooo  1 12     Time of plate single wash  300 pl          ooooccooccco eae max  65 s    Number Of programs  ines esta iaa brad a da ie whine 101     Plate platform and manifold movement     a oa nnana automated    Indication of operationmodes    asasan asama aaa aa a ete 8 line LCD    DIMENSIONS aa ar AAA SE A A a eo OO a 375x345x180 mm     A ii 2 24 EN EE EES E A Ad a 9 6kg    Input current power consumption       oooocooco 00 e eee eee 12V 1 8A 22W   gt  External power supply unit                         inputAC 100 240 V 50 60 Hz  output DC 12 V                                              Optional accessories Description Catalogue number  4 channel washing solution weight logger   4CHW Logger max  loading per scale cup 2 kg  logger dimensions BS 060102 AAI  267x252x97 mm  weight  3 kg   Replacement parts Description Catalogue number  Bottle 1 Assembled  with weight  tube  filter  BS 060102 S26  Bottle 2 Assembled  with weight  tube  filter  BS 060102 S27  Bottle 3 Assembled  with weight  tube  filter  BS 060102 S28  Bottle 4 Assembled with tube BS 060102 S29  Bottle 5 Assembled  with filter  tube  BS 060102 S43  Hydr
6. EI Wao ass  2 g al 2  100  00 00  00  2  Era bg VW S o  3 DO 00 001  O0 ze  5 QQQ QQQQQQQQQ   3      00  DO 100  00 N  E   2009009222220 z 21     DO  DO DO DO ah  E    QQQQQQQQOQQQO  amp  2 2 0O DO G Co   rr  gl    00 00 00 00   000000000000 Ulla    DO   001 OO   00   Scheme 1    The FastFRAME  Schleicher amp Shuell  multi slide plate or analog plate of another manufacturer  that is   compatible with standard 1 x 3 inch  25 x 76 mm  glass slides    acp NOTE  Optional 4 channel washing solution weight logger  4CHW Logger allows automatic    buffer bottles and waste bottle volume control     The unit is supplied with 8 channel washing head for dispensing aspiration  3 bottles for washing and  rinsing solutions  a waste bottle and small bottle with hydrophobic filter that eliminates risk of contamination with  bacteria  viruses and infected particle from wasted liquid to an atmosphere  Hydrophobic filter bacterial  efficiencies is very high  up to 99 999  particles bigger than 0 027 micron  which is smaller than Hepatitis A  B  and C      The unit provides     washing mode     rinsing mode     mixing mode      single point  two point  circular  circle or rectangular path      possibility of additional solution mixing during time gap between two work cycles       possibility to use microtest plates by different manufacturers  ensured by automated plate set up   adjusting to different depths of plate wells        round bottom plate and strip washing mode     possibility of user de
7. MI     Medical Biological  Research  amp  Technologies    Inteliwasher    3D IW8    Microplate washer       for version  V 2AW    Operating Manual  Certificate          Contents    1 Safety PreCaUtiONS   wisc di bl ie tia a 3  2 General information sss isase A A A A a a ai 5  3 Getting started eaei ee e aal RA dadas abaratar brand dida 6  4 Operation eiii iia a die ead bh it 64 Ache de dk la a ed a hele ee 10  5 Operation closedown      2 2 0 0    eee 13  6 Progdram error Messages Vii tae ae Se Sa eS Bee Oe Rhee ee a 14  7 Specifications site enaa dees He A A OWA PONS PURE AR ORAS ASR 15  8 Maintenance and troubleshooting         0    0c eee 16  9 Warranty  Reclamation Information              000000 eee 18  10 GIOSSALY 2544 240444 dr rada a dbeeoeepeera teenais idee dade tedaaed te detains 2 19  11 CGiManking  2  2244 200420 phe tayist on Sadho soem e bee RAR 20  Version 2 04     March 2014 Contents    Page 2    Inteliwasher 3D IW8    1     Safety precautions    A Attention  Please make sure you have fully read and understood the Manual before using the    equipment and pay special attention to sections marked by this symbol     F Note  Paragraphs marked with this symbol contain information about important functions and    use of the device     GENERAL SAFETY PRECAUTIONS    Use only as specified in the Operating Manual provided   Protectthe unitfrom shocks and falling     After transportation or storage  keep the unit at room temperature for 2 3 hours before connecting to th
8. am cycle error messages  their description and possible solutions     A E TRA HOME ERROR   Initalplatiormposiionemor        2  ETRASTEPERROR   Prallommenementemar  gt      4   HEADMOVEUPERROR   Headmovementerror     5  K RESETERROR   Emormovingvalveinto initial position          6    K POSITIONERROR__  Errormovingvalveintosetposition____          7    CANCELED BY OPERATOR   _ Program execution is stopped by operator__   Press Run Stop key _     8    E TRAIL FORERROR   Platformmovementerror          9    E TRAIL BACKERROR   Platform movementerror J       A 1 E RARAS    14   E LANDING ZONEERROR  Error movingplateintoworkingzone       gt  o    19  4CHW Logger error    Table 4  Program error messages         Repair only by service engineers and trained specialists     6  Program error messages Version 2 04     March 2014  Inteliwasher 3D IW8 Page 15    i    Specifications    The unit is designed for operation in closed laboratory rooms at ambient temperature from  4  C to  40  C    in a non condensing atmosphere and maximum relative humidity 80  at 31  C decreasing linearly to 50     relative humidity at 40  C       Dispense system of liquid dosage for each channel separately      Minimumdispensevolume ooo 25 ul    Maximum dispense volume ooo 1600 ul    DISPENSE Incrementa Ad E a 25 ul  o Irregular liquid dosage at 300 Ul    2    o max   2 5  or 7 5 ul     Allowed residual liquid volume in plate well     n n annann max  2 ul     Number of wells washed simultaneously    1    0 0 0
9. ble    plate wells or the   dispensing channel of Dee  washing solution is not the manifold ora 5  Ensure presence of liquid in the bottles No  1  2 and 3  fill up  dispensed at all  needle liquid in a bottle if needed   Attention  information on liquid  ae  levels in bottles is shown on the display    5  Absence of liquid in  bottles  Table 5  Troubleshooting  8  Maintenance and troubleshooting Version 2 04     March 2014    Inteliwasher 3D IW8 Page 17       Symptom    Possible cause    Action required       Obstruction of manifold  dispensing channel or  a needle    During operation needles  of the manifold can be  obstructed     1  Determine the obstructed needle by checking the underfilling of  awell    Warning   Be careful working with the manifold  prevent pricks and  injuries caused by needles  they may contain dangerous  infections    2  Take off the manifold from the device  put it down with needles  upward  Clean the aspiration channel needles with the wiping   rod   0 80 mm  and clean needles of the dispensing channel  with the wiping rod    0 45 mm  inserting the wiping rod against  stop and removing it    3  After cleaning  install the manifold on the device and do the  rinsing procedure to ensure the proper operation of the needle    4  In the case of repeated obstruction  execute requirements of pp   1 and 2 of this section  unscrew the manifold   s plugs  disconnect  the dispensing and aspirating tubes  clean the dispensing or  aspiration channel with the wipi
10. e  changes and startthe unitoperation     WASHING PROGRAM WASHING PROGRAM    Bottle  cycles Second stage rinse    shake from bottle 02  bottle  1  2  3    51 42 30 Press RUN button       Fig  8  Operating program messages Fig  9  Operating program messages    During operation  the number of cycles left is displayed  fig  7   If the current action has time tracking   soaking  shaking   then the elapsed time is displayed     If 4 channel washing solution weight logger is connected to the unit  the display during operation shows  following massages  fig  8   program number  active bottle number  cycles left and the remaining volume  percentage of liquid in the bottles  For the waste bottle  filled liquid amount is displayed accordingly  based on 2 litre bottle volume percentage     Pressing the Run Stop key during operation will stop the program  and the message    Canceled by  operator    appears on the lower line ofthe display  Press the Run Stop key to restartthe washing     Note  If operation requires washing from one channel  parameter On two channels set to NO    washing parameters for the second channel are not displayed  Respectively  if two  chamnels are set  parameter On two channels set to YES   washing parameters for the  second channel are displayed and can be adjusted as needed  Table 3   Before starting  the program for washing from two channels  the message  to confirm the correct bottle  number of second wash  appears on the display  fig  9      Version 2 04    
11. e  mains     Use only cleaning and decontamination methods recommended by the manufacturer   Do not make modifications to the design ofthe unit     Before diagnostics  study carefully the possibilities and operation procedures of the unit in order to obtain  reproducible and credible results  Check any unclear issues with the supplier     Itis not recommended to leave the operating unit unattended for a long period   Change the bottles or connecting tubes only when the unitis switched off     In case of doubt about the quality and uniformity of the completed washing program  disregard the  results obtained and repeat the washing procedure    Accuracy of the obtained testing results depends on the quality of the microplate rinsing procedure  To  assure correctness of the rinsing procedure  regularly exercise visual control over reproducibility of liquid  volume dosing     ELECTRICAL SAFETY    Connect only to an external power supply unit with voltage corresponding to that on the serial number  label  Use only the external power supply unit provided with this product     Do not plug the power supply unit into an ungrounded power socket  and do not use an ungrounded  extension lead     Ensure that the switch and external power supply connector are easily accessible during use   Disconnect the unit from the mains before relocating it     If liquid penetrates into the unit  disconnect it from the mains and do not use it until it is checked by a  repair and maintenance technician  
12. escending    Poor contact of the  manifold with the holder  arm    Wipe the golden contacts of the manifold and the holder arm with  a piece of cotton moistened with 75  ethanol solution        On switching on  the  device does not fetch  liquid from the active  bottle           Insufficient liquid  quantity in the pipe          To solve this problem  the following procedure should be done     Stop the programme      Prepare a syringe with 5ml of distilled water      Unscrew the hose from the active bottle      Press a button    System Rinse          Using buttons     and      select number of the active bottle  and press the button    Start          At the moment when the pump begins operation  gradually fill  the hose with distilled water from the syringe       Tighten the hose on the bottle       If the pump does not restore performance  turn to the service  engineer                 Version 2 04     March 2014  Page 18    Table 5  Troubleshooting    8  Maintenance and troubleshooting  Inteliwasher 3D IW8    9 1     9 2     9 3     9 4     9  Warranty  reclamation information    Warranty  reclamation Information    The Manufacturer guarantees the compliance of the unit with the requirements of Specifications     provided the Customer follows the operation  storage and transportation instructions     The warranted service life of the unit from date of delivery to the Customer is 12 months  exclude items  mentioned in the table of Specifications point   Contact your local dis
13. f the box between the  packaging blocks and the side panels of the carton box  place the 2 litre bottle and the half litre bottle in  free space  between the packaging blocks and the side panels of the carton box     Insert the top section into the carton box  previously bending it downwards along the perimeter and insert  the deflected parts between the walls of the original carton box and packaging blocks  so that the device  and the rest content of the lower part of the box are fully covered     Put the soft foam sheet  of grey colour  with the smooth side downwards on the resulting horizontal  carton surface     Putthe rest three bottles and other accessories on the foam sheet     Note  If 4 channel weight logger is included in the kit  place pads under scale cups  then place  the logger into top section of packaging     Cover all the content with another sheet of soft foam material with the smooth side upwards   Close the box and seal with duct tape   Unit on site installation and preparing for operation    Place the unit on a strong horizontal surface  which can safely support weight of the unit  To provide  optimal ventilation  ensure 100 mm clearance on each side  Operate at ambient temperature from  4   to   40  C and relative humidity up to 80  at  25  C     Remove the protecting cover   Unpack the plate platform     Place the plate platform on the rail  fig  2 1  so that the plate holder  flat spring  faces the rear side of the  unit  fig  2 2   Magnet on the other
14. fined programs with adjustable parameters     2  General information Version 2 04     March 2014  Inteliwasher 3D IW8 Page 5    3 1    cr    3 2    Getting started    Unpacking     Remove packaging materials carefully and retain them for future shipment or storage of the unit     Note     Examine the unit carefully for any damage incurred during transit  The warranty does not  cover in transit damage     Complete set  The set includes   No  in Fig 1    1    ON DOO BW DY     o    11  12  13  14  15  16  17  18       NaMe ssarrerrerroredea Peas de aba oe ne We end vee BOW Yaa Quantity  Inteliwasher 3D IW8 microplate washer                00 00 e eee eee eee 1 piece  Platform tor plates  vosotras Oa eee a ad ee A ee ae oa 1 piece  Manitold 222240040 ad arca drid a rd dee ee heed 1 piece  Protecting COVED sic wade a mak Rink ea RIA a e 1 piece  External power supply unit ac  eass taras tadaa aa a a a e eee 1 piece  Tubes  outside inside diam  length 6 3 600 MM             00  cee eee 5 pieces  Tube for manifold  outside inside diam  length 3 2 1 6 400 mm                  0  1 piece  Tube for manifold  outside inside diam  length 5 3 440 mm               0000 eee 1 piece  Tube for hydrophobic filter  outside inside diam 9 6mm            000 0c eee 1 piece  Half litre bottle with connectors for aerosol collectiON                o o o o o oooo    1 piece  1 litre bottles with sieve filters and connectors for reagents              000000 ee 3 pieces  2 litre bottle with connector for
15. ion Version 2 04     March 2014  Inteliwasher 3D IW8 Page 13    5 1     Operation closedown    When the cycle is completed  a sound signal will inform thatthe unit stopped the operation     Attention  Always perform the rinsing cycle using distilled water after finishing the operation at the  end of working day  This will help to keep the unit in working order and prevent manifold  channel clogging        5 2  Press Prime rinse  fig  10 1   and the message  PRIME RINSE  the channel 01  will appear    Select the correct channel using     and      fig  10 2    Press Run Stop  fig  10 3  key and the system will perform rinsing cycle   Repeat the procedure twice  if necessary    5 3   Disconnectthe tube from selected bottle  and press Prime rinse key again to dry the tube with air    5 4  After finishing the operation  remove the tube from the valve opening to prevent deformation  wall  glueing  of the dosing valve tube  To remove the tube  press the valve on the side  fig  2 4  2 f  and stretch  the tube a little when pulling it through an opening    5 5  Switch the unit off using mains switch on the rear panel  position O  OFF     5 6  Unplug the external power supply unit from the mains    PRIME RINSE   The channel 01   Press RUN button  Prime Program   rinse parameters Enter   Back     Run   Row Stop 3   2  Fig  10  Prime rinse function  Version 2 04     March 2014 5  Operation closedown    Page 14    Inteliwasher 3D IW8    6  Program error messages    Table 4 shows the progr
16. ird category  IPF96 FLAT C  are intended for flat bottom well  immunoplates       Fourth category  FastFRAME 2  and fifth category  FastFRAME C  are intended for Watman Schleicher   amp  Shuell FastFRAME plates with rectangular wells     o1   02   03   04   05   o   07  08 100    Z  S                                            i      3   E  Bl e   3 8 3  gt   I   2 E  E   l l l l    c     s       8        2    amp  3 5      0 x x x x   a 2  A  lt       ES irs  ts  a A    Soakings   20   30   30   30   30   30   300   oo   00      Shakings_____                               30   30      _ Dispensedvolume  pI       400   400   400   400   400   300   200   300      Aspiratetimems   1200   600   600   600   600   600   600       1000      Finalaspirate ms___        1200   1200   1200   1200   1200   1200       YES      Firstaspirate        YES   YES   YES   YES   YES   NO       YES      Washbyrows       YES   YES   YES   YES   YES   NO       YES      Ontwochannels       NO   NO   NO   NO   YES   NO    Num of1 wash cycles       01   02   03   05   05   o    1 channel Pp      on   ot   ot    aja  u    Num of2wash cycles                       o1        2 channel  gt                   S  da   o       Table 1  Initial parameters of all user programs    4 3  Install the plate on the platform   Attention  When installing a plate the first time or when a different type of plate is installed perform  A plate setup to adjustthe manifold lowering depth  see paragraph 4 20    Note  When wor
17. king with the FastFRAME plates  remove the plate holder  fig  2 2   Install it    back when working with immunoplates     4 4  Use the Enter  fig  5 1  key to select category  depending on the used plate type  Use the     and     keys   fig  5 3  to select required washing program from 1 to 10  Program category and number are displayed in  the top right corner of the display  fig  4 1     4 5  Press the Program Parameters key  fig  5 4  to view the parameters of program  In the program  first  parameter is selected  Dispense   yes no  fig  5 5      4 6  Press the Enter key to select next parameter     Version 2 04     March 2014 4  Operation  Page 10 Inteliwasher 3D IW8    Plate shaking on the platform during the cycle    Firstaspirate    25   1600  step 25 Volume of dispensed liquid    Second chan  cycles 01  15 Number of washes with second solution  Second channel 01  03 Number of second bottle to use    Table 2  Programs and their definitions        During aspiration  the waste fluid bottle is detected automatically   4    bottle  The number of the bottle is  not specified on the display     4 7  Use the     and     keys to select necessary value and press the Enter key to change the active  parameter  Pressing the Enter key automatically saves the setting and activates the next parameter  This  way you will be able to review and or change all parameters     4 8  Pressing the Back row key  fig  5 6  will return to the previous parameter in the program     4 9  Press the Pr
18. lter  its  IN  marking must be facing the  bottle 5  fig  11      3 4 11  Connect the Waste bottle to the half litre bottle  number 5   fig 3 10     On the rear side of the unit    3 4 12  Connect bottle with buffer 1 to the inlet connector  1     red  on the rear side  fig  3 3  with the tube  fig  1 6    3 4 13  Connect bottle with buffer 2 to the inlet connector  2     black   fig  3 5  with the tube  fig 1 6     3 4 14  Connect bottle with buffer 3 to the inlet connector  3     white   fig  3 6  with the tube  fig  1 6      3 4 15  Insert the manifold tube  fig  3 2  into the valve opening  fig  2 4   Press the valve in the direction of the  arrow and hold the valve while stretching the tube and pulling it through the opening  Release the valve                                                                                                                                                                                Fig  3  Assembly diagram    Version 2 04     March 2014 3  Getting started  Page 8 Inteliwasher 3D IW8    CF    3 4 16   3 4 17     ar    3 4 18   3 4 19     A    3 5    3 5 1   3 5 2   3 5 3   3 5 4   3 5 5     Note  Make sure that all connections are secured tightly   Install the protective cover ensuring all tubes are covered  but not squeezed   Place bottles near the unit     Note  If 4 channel weight logger is included in the kit  remove pads from underside of scale  cups     Place bottles on the 4 channel weight logger according to the numbering   Connec
19. ng rod  rinse the manifold with  the 75  solution of ethanol  replace the plugs  install the  manifold on the device and ensure the proper operation of  needles        Washing solution is not  dispensed into a plate    Deformation  wall  glueing  of dosing valve  tube due to the long term  storage or standstill of  the device    1  Remove the tube from the valve     2  In the case of deformation  open with fingers the closed part of  the dosing valve    3  Insert the tube into the valve opening up to position shown in  Fig 2 4    Note  in order to release the tube  press the valve on the side and  slightly stretch the tube pulling it through the opening        Wear or damage of the  valve tubes and the pump    The connecting tubes are  worn or damaged during  exploitation     Turn off the device and have it checked by a specialist     Only service engineers and trained specialists are allowed to  replace the tubes        Volume of residual  liquid in a well exceeds  the admissible value  2  mkl      1  Depth of the manifold  immersion is not  determined    2  Obstruction of the  manifold aspiration  channel or a needle    1  Perform well depth measurement in the automatic mode     2  Check if the aspiration channel and needles are not  obstructed  If yes  do their cleaning  see the section     Obstruction of manifold dispensing channel or a needle    in  this Table      If it does not help  turn off the device and have it checked by a  specialist        The manifold is not  d
20. ogram Parameters key again to exit parameter view and change mode and save the  program     4 10  Press the Esc key  fig  5 7  to exit parameter view and change mode without saving   4 11  Usethe Run Stop key to start the program          WASHING PROGRAM  IPF96 U V    C  IPF96 FLAT 2  IPF96 FLAT C           FastFRAME 2  FastFRAME C  PLATE SETUP        ENTER to select    Fig  4  Program selection       4 1 7  5 Prime Program   rinse paramete Enter Esc  Aspirate C  C   Shake    Dispensing rate  Aspirate rate  Soak llimit  s   Back al Run   Row Stop   2  Fig  5  Control panel  4  Operation Version 2 04     March 2014    Inteliwasher 3D IW8 Page 11    4 12     4 13   4 14   4 15    4 16     4 17     4 18     WASHING PROGRAM WASHING PROGRAM    Bottle  Number of rows cycles    shake  Press RUN button       Fig  6  Pre set rinsable row number Fig  7  Operating program messages    If washing of less than 12 rows on a plate is needed  press the Rows key before starting  The following  message appears on the display  fig  6      Select necessary number of rinsable rows using     and     keys   Pressing the Run Stop key will save changes and start the unit operation     Ifoperation requires washing from two channels  parameter On two channels setto YES   the message   to confirm the correct bottle number of second wash  appears on the display before starting the program   fig  9     Select necessary bottle number with buffer using     and     keys and press the Run Stop key  It will sav
21. ophobic filter For bottle 5 BS 060102 S44  Filter complete set Filter  weight  tube BS 060102 S01  Silicone tube set 6 pcs  BS 060102 S39                  Accurate within 10      Biosan is committed to continuous improvement of the unit s properties and quality and reserves the right    to alter unit s design and specifications without additional notice     Version 2 04     March 2014 7  Specifications    Page 16    Inteliwasher 3D IW8    8 1   8 2   8 3     8 4   8 4 1     8 4 2     8 5     8 6     A    8 7     Maintenance and troubleshooting    If the unit requires maintenance  disconnect the unit from the mains and contact your local Biosan  representative or Biosan service department     All maintenance and repair operations must be performed only by qualified and specially trained  personnel     Do not use non conforming parts for repair  The manufacturer provides all necessary services and spare  parts  To order necessary services and parts  please contact your supplier     Maintenance checks  The following checks can be done by operator   Daily maintenance   clean the surface of the device from liquid droplets and contamination   perform the rinsing cycle twice using distilled water after finishing the operation  see point 5    Monthly maintenance   Cleaning with 75  ethanol  e  mounting contacts on the manifold                 mounting surface ofthe plate platform and plate holder spring     Ethanol  75   or other cleaning agents recommended for  cleaning of laborator
22. rming one by one the program parameters    command to setthe number ofwashable rows  command to stop the washing program    10  Glossary  Inteliwasher 3D IW8    11  CE Marking    The C    marking affixed to the equipment indicates that the equipment meets the requirements of the following  Directive s      RoHS Directive 2011 65 EU     RESTRICTION OF HAZARDOUS SUBSTANCES       WEEE Directive 2002 96 EU and 2012 19 EU     WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT       EMC Directive 2004 108 EC     ELECTROMAGNETIC COMPATIBILITY     Applied standards     EN 61326 1 Electrical equipment for measurement  control and laboratory use EMC requirements   General requirements     Low Voltage Directive 2006 95 EC     ELECTRICAL EQUIPMENT DESIGNED FOR USE WITHIN CERTAIN VOLTAGE LIMITS     Applied standards     EN 61010 1 Safety requirements for electrical equipment for measurement  control and laboratory  use  General requirements     11  CE Marking Version 2 04     March 2014  Inteliwasher 3D IW8 Page 21    Biosan SIA   Ratsupites 7  build 2  Riga  LV 1067  Latvia  Phone   371 67426137 Fax   371 67428101  http   www biosan lv    Version 2 04     March 2014  Inteliwasher 3D IW8    
23. s  potentially dangerous biological materials can be used  When working with    such materials  always use protective clothing and eye protection  Always have the protective cover  installed during operation     Version 2 04     March 2014 1  Safety precautions    Page 4    Inteliwasher 3D IW8    2  General information    Inteliwasher 3D IW8 series microplate washer is designed for washing various types of standard  microtitre plates  microstrips and microarrays on FastFRAME  rectangular well shape   It is suitable for washing  wells with different bottom shapes  flat  U shape and V shape  The unit is fully programmable ensuring multi step  solution ripening  aspiration  aspiration  combination of aspiration liquid dispensing and soaking  as well as  soaking cycle during a particular period of time      The unit has 50 programs divided into 5 following aspiration categories  see scheme below    D Type 1  1 0   1 9  IPF96 U V is intended for U shape and V shape immunoplates  1 point aspiration      2  Type 2  2 0   2 9  IPF96 FLAT 2 is intended for flat bottom shape immunoplates  2 point aspiration        Type 3  3 0   3 9  IPF96 FLAT C is intended for rectangular shape immunoplates  full circle aspiration  direction      4  Type 4  4 0   4 9  FastFRAME 2 is intended for multi slide  plate with rectangular wells        Type 5  5 0   5 9  FastFRAME C is intended for multi slide  plate with rectangular wells                                                                       T
24. t the RS 232 with the cable to the plug connector on the rear side of the unit  fig  3 7      Attention  Make sure that the power switch of the unit is in O  Off  position before connecting power  cable to the mains outlet     Switching on   Connect the external power supply to the power socket  fig 3 8  on the rear panel of the unit    Connect the power cord to the external power supply unit  fig  1 16    Connect the external power supply unit to the grounded power socket    Switch ON the unit  position I  using the power switch on the rear panel    When switched on  the unit performs full initialisation cycle and displays the message  Power on reset     Note  Fullinitialisation cycle takes not longer than 5 seconds     3  Getting started Version 2 04     March 2014  Inteliwasher 3D IW8 Page 9    4  Operation    4 1  Before starting plate washing   Fill the rinsing liquid bottle  number 3  with distilled water or appropriate cleansing solution   Fill the necessary bottles  number 1 and or 2  with washing solutions     4 2 Initial parameters of all programs are given in Table 1  All values are adjustable  Ranges descriptions of  program parameters are shown in Table 2  All programs are divided into 5 categories of 10 adjustable  programs  Each category corresponds to a different plate well configuration and  subsequently   aspiration mode  fig  4       First category  IPF96 U V  is intended for U shape and V shape well immunoplates        Second category  IPF96 FLAT 2  and th
25. tributor to check availability of    extended warranty     If any manufacturing defects are discovered by the Customer  an unsatisfactory equipment claim shall  be compiled  certified and sent to the local distributor address  Please visit www biosan  v  Technical    support section to obtain the claim form     The following information will be required in the event that warranty or post warranty service comes  necessary  Complete the table below and retain for your record        Model    Inteliwasher 3D IW8       Serial number          Date of sale             Inteliwasher 3D IW8    Version 2 04     March 2014  Page 19    10  Glossary    Version 2 04     March 2014    Page 20    O ON DO       o    12    Rinsing mode    Mixing mode  Single point aspiration    Double aspiration    Strip washing mode  Dispensing   Waste bottle   Plate setup   Run mode   Enter    Rows  Stop mode    plate washing mode consisting of consequent dispension  and aspiration to from wells     provides mixing of solution in wells by shaking the platform    plate washing mode which provides solution aspiration  from the center point ofa well     plate washing mode which provides solution aspiration  consequently from two opposite points of a well    plate washing by rows   filling wells with a set solution volume   the bottle for collecting the aspirated liquid  procedure for automatic measurement of well depth  command to startthe washing program    in program setup mode  this command is used for  confi
26. y equipment can be used for cleaning and  disinfection ofthe unit                                      Filter replacement  fig  11                    It is recommended to change the hydrophobic filter once in six        months  in particular cases  e g  at intensive operation  once in  three months  to order the filter  you have to know the product  identification number  see the table in Specifications chapter      Disconnect the filter from outlet tube  remove the filter  insert a  new filter into the socket and tighten it                 Attention  Maintain mounting of the filter according to is  Fig 11  The marking    IN    should be located on the    side of the inlet tube  facing to the bottle   Fig  11  Filter mount    Troubleshooting                         Symptom Possible cause Action required  Manifold dosing error   1  Poor contact between   1  Ensure proper connection of the bottle with the device or  the bottle hose con   Volume of washing nector and the device 2  Check if the hose is overbend and straighten it if necessary or  solution does not 2  The hose is overbend   3  Check if the sieve filters in bottles No  1  2 and 3 are clogged   correspond to the set i       E F  volume  dosing 3  Obstruction of sieve 4  Check if the dispensing channel of the manifold or needles is  unevehness is filters not obstructed  If yes  perform cleaning  see the section  b dal th      Obstruction of manifold dispensing channel or a needle    in  nl Ai 4  Obstruction of this Ta
    
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