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ÄKTA avant Installation Guide

1. Chemical Concen CAS no EC no tration Acetone 10 67 64 1 200 662 2 Rare RPC Ammonia 30 7664 41 7 231 635 3 Oligonucleotide syn thesis Ammonium chloride 2M 12125 02 9 235 186 4 Hydrophobic Interac tion Chromatogra phy HIC Ammonium 3M 7783 20 2 231 984 1 Cleaning of plas sulphate mids HIC Arginine 2M 74 79 3 200 811 1 Wash and elution pH 4 using protein A media refolding Benzyl alcohole 2 100 51 6 202 859 9 Cleaning and stor age of columns Decon 90 10 N A Cleaning Dimethyl sulphoxide 5 67 68 5 200 664 3 CIP RPC cell separa DMSO tion Dithiothreitol DTT 100mM 3483 12 3 222 468 7 Reducing agent Dithioerythritol DTE 100 mM 6892 68 8 229 998 8 Reducing agent Ethylenediaminete 100mM 60 00 4 200 449 4 Buffer additive traacetic acid EDTA Ethanol 20 75 08 1 200 837 3 Storage Note Long term use 1 month Ethanol 96 75 08 1 200 837 3 CIP Ethanol NaOH 40 N A CIP 1M Ethylene glycol 50 107 21 1 203 473 3 HIC elution Formic acid 1 64 18 6 200 579 1 RPC peptide separa tion Glycerol 50 56 81 5 200 289 5 HIC KTA avant Installation Guide 29 0351 82 AA 10 Reference information 10 4 Chemical resistance guide Chemical Concen CAS no EC no tration Glycine 2M 56 40 6 200 272 2 Cleaning of MAb binding media Guanidinium 6M 50 01 1 200 002 3 Denaturizing agent hydrochlo
2. AKTA avant Installation Guide 29 0351 82 AA 15 2 Introduction to KTA avant 2 2 Indicators and controls Indicator Button Description Indicates that fractionation is ongoing Do not open the Frac drawer during fractionation 16 KTA avant Installation Guide 29 0351 82 AA 2 Introduction to AKTA avant 2 3 Connectors 2 5 Connectors Introduction This section describes the connectors for power and communication on the AKTA avant instrument Illustration The illustration below shows the location of the connectors 1 1 TITU peed a 303 geeseecesaged teee poen ob KT eee Part Function 1 Power input connector 2 Network connector Ethernet 3 UniNet 9 connectors Note Termination plugs must be connected to the connectors that are not in use Other connectors are for use by authorized service engineers only NOTICE gt Misuse of UniNet 9 connectors The UniNet 9 connectors at the rear panel should not be mistaken for Firewire connectors Do not connect any external equipment to the UniNet 9 connectors Do not disconnect or move the UniNet 9 bus cable KTA avant Installation Guide 29 0351 82 AA 17 2 Introduction to AKTA avant 2 4 Network architecture 2 4 Network architecture Introduction The AKTA avant instrument is connected to the UNICORN computer through the Network connector Ethernet at the back of the instrument There are two possibl
3. 280 270 260 250 240 230 220 210 200 190 180 170 160 150 140 130 120 110 100 30 80 70 60 50 40 30 20 10 10 UV1_265_Chrom 1 System Test 001 UV 2_254_Chrom 1 System Test 001 UV 3_280_Chrom 1 System Test 001 Cond_Chrom 1 System Test 001 Conc B_Chrom 1 System Test 001 ml 30 40 50 60 70 80 30 100 110 120 130 140 150 160 170 180 Gradient test result Step response test resul Sample peak and Conductivity test result Note The illustration shows a chromatogram from a System test on KTA avant 25 A chromatogram from a System test on KTA avant 150 has a similar appear ance but a different scale on the y axis KTA avant Installation Guide 29 0351 82 AA 99 9 Performance tests 9 4 System test 9 4 3 Evaluate the test 9 4 3 Evaluate the test Automatic evaluation The system automatically generates a report when the test is finished The report can be printed in two ways e Ifthe option Save the report to file was selected in the Evaluation Procedures dialog in the Start Protocol of the test the report is saved in location C Program Files GE Healthcare UNICORN Temp Open the report and print it using your default Windows printer Recommended Note The exact search path depends on the location of the UNICORN installation folder e If the option Print report was selected in the Evaluation Procedures dialog in the Start
4. 60 C Chemical environment See Section 10 4 Chemical resistance guide on page 124 Operating range Parameter Data Operating temperature range 4 C to 35 C Relative humidity 20 to 95 non condensing KTA avant Installation Guide 29 0351 82 AA 113 10 Reference information 10 2 Component specifications 10 2 Component specifications Introduction This section specifies the operating data of the components in AKTA avant For general data for the system see Section 10 1 System specifications on page 112 System pumps Parameter Data Pump type Piston pump metering type Flow rate range KTA avant 25 0 001 to 25 ml min KTA avant 150 0 01 to 150 ml min Pressure range KTA avant 25 0 to 20 MPa 2900 psi KTA avant 150 0 to 5 MPa 725 psi Viscosity range KTA avant 25 0 35 to 10 cP KTA avant 150 0 35 to 5 cP Flow rate specifications KTA avant 25 e Accuracy 1 2 e Precision RSD lt 0 5 Conditions 0 25 to 25 ml min lt 3 MPa 0 8 to 2 cP KTA avant 150 e Conditions 1 0 to 150 ml min lt 3 MPa 0 8 to 2 cP e Accuracy 1 5 e RSD lt 0 5 114 KTA avant Installation Guide 29 0351 82 AA Sample pump 10 Reference information 10 2 Component specifications Parameter Data Pump type Piston pump metering type Flow rate range KTA avant 25 0 01 to 25 ml min KTA
5. Click Next in the dialogs of the Start Protocol to open the next dialog The dialogs are described in table Overview of the Start Protocol on page 91 In the last dialog of the Start Protocol Result name and location click Finish Result The Q valve test starts Overview of the Start Protocol The table below describes the dialogs of the Start Protocol Dialog Description Notes Displays the Method Notes of the method The Method Notes contains a method description and instructions on how to run the method This dialog also allows the user to enter Start Notes Evaluation Procedures Allows the user to select to save the report to file recom mended and or to print the report tion Result Name and Loca Allows the user to change result name and result location AKTA avant Installation Guide 29 0351 82 AA 91 9 Performance tests 9 3 Q valve test During the run A Message dialog opens during the run Read the messages in the dialog and make sure that necessary preparations have been performed Click the Confirm and Continue button in the Message dialog to change system state from System Pause to Run and proceed with the test Alternatively click the Confirm button in the Message dialog and click the Continue button on the Instrument display Automatic evaluation The system automatically generates a report when the test is finished The report can be printed in two ways e Ifthe opt
6. Required material The following material is required e Distilled water e Syringe 25 30 ml e For KTA avant 25 Cassettes for deep well plate 2 pcs 96 deep well plates 2 pcs e For KTA avant 150 Cassettes for 50 ml tubes 2 pcs 50 ml tubes 12 pcs Prepare the test Follow the instruction below to prepare the system before method start Step Action 1 Immerse the piece of inlet tubing marked A1 in distilled water 2 Prime inlet A1 and purge System pump A See Section 8 1 Prime buffer inlets and purge System pump A and System pump B on page 63 3 Place the deep well plates or tubes in the cassettes Make sure that the deep well plates are rotated so that the well marked A1 is positioned above the A1 marking on the Cassette AKTA avant Installation Guide 29 0351 82 AA 85 9 Performance tests 9 2 Fraction collector test Step Action 86 Place the Cassettes on the Cassette tray one on Cassette position 1 and one on Cassette position 6 Make sure that the Cassette type codes see il lustration below faces the front of the tray marked with the GE logo C SY Open the Frac drawer by pressing the handle upwards and pulling out the drawer KTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 2 Fraction collector test Step Action 6 Place the Cassette tray on the Tray support of the Frac drawer Make sure that the front of the tray marked with the G
7. e Ifthe baseline is not drifting Integrate the curve using the function Zero baseline e If the baseline is drifting Correct the baseline before integrating the curve For further information on peak integration see Evaluation Manual 3 Enter the area of each peak in column 2 in table Sample peak and conduc tivity test result on page 110 Add the areas of peak 1 and peak 2 and divide the area of each peak with the total peak area Enter the results in column 3 4 Check that the obtained percentages fall within the intervals given in column 4 104 KTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 4 System test 9 4 4 Possible causes of a failed test 9 4 4 Possible causes of a failed test Introduction The tables in this section describe possible causes of a failed test When possible sources of error have been checked and taken care of run the test once again Faulty Gradient Test Result Disturbances caused by air trapped in any of the pumps Make sure to prime the buffer inlets and to purge the System pumps and the Sample pump before method start See Section 8 1 Prime buffer inlets and purge System pump A and System pump B on page 63 and Section 8 2 Prime sample inlets and purge the Sample pump on page 71 Disturbances caused by damaged pump piston seals Replace piston seals See User Manual chapter Mainte nance Unstable or incorrect UV signal or drifting base line Fau
8. 29 0351 82 AA 27 3 Site preparation 3 4 Site environment 3 4 Site environment Introduction This section describes the environmental requirements for installation of AKTA avant Room climate The following requirements must be fulfilled e The room must have exhaust ventilation e The instrument should not be exposed to direct sunlight e Dustin the atmosphere should be kept to a minimum Allowed temperature and humidity ranges are specified in the table below Parameter Allowed range Ambient temperature operating 4 C to 35 C Ambient temperature storage 25 C to 60 C Relative humidity operating 20 to 95 non condensing Heat output The heat output data is listed in the table below Component Heat output AKTA avant instrument 800 W Computer incl monitor and printer 300 W Total 1100 W 28 AKTA avant Installation Guide 29 0351 82 AA 3 Site preparation 3 5 Power requirements 3 5 Power requirements Introduction This section describes the power supply requirements for AKTA avant WARNING Protective ground The AKTA avant instrument must always be connected to a grounded power outlet WARNING Only use grounded power cords delivered or approved by GE Healthcare WARNING Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect WARN
9. 46 4 3 Connect system units 47 4 4 Install the instrument 50 AKTA avant Installation Guide 29 0351 82 AA 35 4 Hardware installation 4 1 Unpack the instrument 4 1 Unpack the instrument Introduction This section describes how to unpack the AKTA avant instrument and how to lift the in strument onto the bench WARNING Heavy object The AKTA avant instrument weighs about 116 kg Use proper lifting equipment or use four or more persons when moving the instrument All lifting and moving must be performed in accordance with local regulations 36 AKTA avant Installation Guide 29 0351 82 AA 4 Hardware installation 4 1 Unpack the instrument Lift the instrument onto the bench using an overhead crane It is recommended to use an overhead crane when lifting the AKTA avant instrument Follow the instruction below to lift the instrument onto the bench using an overhead crane and to remove the transport fixations If an overhead crane is not available see Lift the instrument onto the bench by hand on page 38 Step Action 1 Lift off the cardboard hood 2 Make sure that the safety hooks of the straps are properly connected to the lifting yokes located in each of the four corners of the plywood board under the instrument 3 Connect both straps to the hook of the overhead crane KTA avant Installation Guide 29 0351 82 AA 37 4 Hardware installation 4 1 Unpack the instrument Step Action 4 Li
10. Outlet valve Injection mark Monitors Fraction collection v Save result as Browse V Auto update of parameters during run Click L_Execute_ Result Inlet valve B switches to the selected port 3 Connect a 25 30 ml syringe to the purge valve of one of the pump heads of System pump B Make sure that the syringe fits tightly into the purge con nector 4 Open the purge valve by turning it counter clockwise about 3 quarters of a turn Draw liquid slowly into the syringe until the liquid reaches the pump 64 AKTA avant Installation Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 1 Prime buffer inlets and purge System pump A and System pump B Step Action 5 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 6 Repeat steps 2 5 for each piece of inlet tubing B that is to be used during the run Prime inlet tubing A Follow the instruction below to fill all A inlet tubing to be used in the run with appropriate buffer solution Step Action 1 Make sure that all A inlet tubing that is to be used during the method run is immersed in the correct buffers 2 In the Manual instructions dialog e Select Flow path Inlet A e Select the Position of the inlet to be filled from the drop down list click Bate Result Inlet valve A switches to the selected port 3 Connect a 25 30 ml syringe to the purge valve of one of the pump heads of Syst
11. Title 2006 42 EC Machinery Directive MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive 1999 5 EC Radio Equipment and Telecommunications Terminal Equipment R amp TTE Directive CE marking ce The CE marking and the corresponding EC Declaration of Conformity is valid for the in strument when it is e used as a stand alone unit or 8 AKTA avant Installation Guide 29 0351 82 AA 1 Introduction 1 2 Regulatory information e connected to other GE Healthcare instruments or e connected to other products recommended or described in the user documentation and e usedinthe same state as it was delivered from GE Healthcare except for alterations described in the user documentation International standards This product fulfills the requirements of the following standards Standard EN ISO 12100 Description Safety of machinery General princi ples for design Risk assessment and risk reduction Notes EN ISO standard is har monized with EU direc tive 2006 42 EC EN 61010 1 IEC 61010 1 UL 61010 1 CAN CSA C22 2 No 61010 1 Safety requirements for electrical equipment for measurement con trol and laboratory use EN standard is harmo nized with EU directive 2006 95 EC EN 61326 1 IEC 61326 1 Emission accord ing to CISPR 11 Group 1 class A Electrical equipment for measure ment control and laboratory u
12. account e Room temperature and limited overpressure is assumed Note Chemical influences are time and pressure dependent Unless otherwise stated all concentrations are 100 List of chemicals Proposed chemical compatibility for AKTA avant All chemicals used for CIP and cleaning are for short term use only ambient temperature lt 25 C if not other stated Note A user can be exposed to large volumes of chemical substances over a long time period A Material Safety Data Sheet MSDS provides the user with infor mation regarding characteristics human and environmental risks and preven tive measures Make sure that you have the MSDS available from your chemical distributor and or databases on the internet Chemical Concen CAS no EC no tration Aqueous buffers N A Separation pH 2 12 Acetic acid 70 64 19 7 200 580 7 Cleaning In Place CIP Acetonitrile 1 100 75 05 8 200 835 2 Reversed Phase Chromatography Note Quaternary RPC valve is not resistant Depending on pres sure tubing between pump head and pressure monitor needs to be changed See User Manual chapter Prepare the system for a run Acetonitrile THF 85 15 109 99 9 203 726 8 RPC Note Quaternary Tetrahydrofuran valve is not resistant AKTA avant Installation Guide 29 0351 82 AA 125 10 Reference information 10 4 Chemical resistance guide 126
13. and Technical Manual for further information on network settings AKTA avant Installation Guide 29 0351 82 AA 49 4 Hardware installation 4 4 Install the instrument 44 Install the instrument Introduction This section describes how to install the Barcode scanner and the pH electrode and how to prime the pump piston rinsing systems Install the 2D Barcode scanner Connect the cable of the 2D Barcode scanner to the scanner and to a USB port on the computer Install the pH electrode If pH monitoring is to be used you need to replace the dummy electrode mounted at delivery with a pH electrode CAUTION pH electrode Handle the pH electrode with care The glass tip may break and cause injury Follow the instruction below to install the pH electrode Step Action 1 Unpack the pH electrode Make sure that the electrode is not broken or dry 2 Unscrew the dummy electrode from the flow cell 3 Pull off the plug from the connector on the front of the pH valve and store the plug together with the dummy electrode 4 Remove the cover from the tip of the pH electrode 5 Carefully insert the electrode in the flow cell Tighten the locking ring by hand to secure the electrode 6 Connect the pH electrode cable to the connector on front of the pH valve 50 KTA avant Installation Guide 29 0351 82 AA 4 Hardware installation 4 4 Install the instrument Illustration of the pump piston rinsing syst
14. and tips Note A Note is used to indicate information that is important for trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware controls indicators and connections are identified in the text by bold text e g Power switch Text entries that UNICORN generates or that the user must type are represented by a monotype typeface e g Program Files GE Healthcare UNICORN bin UNI CORN Instrument Server exe config AKTA avant Installation Guide 29 0351 82 AA 7 1 Introduction 1 2 Regulatory information 1 2 Regulatory information Introduction This section describes the directives and standards that are fulfilled by AKTA avant Manufacturing information The table below summarizes the required manufacturing information For further infor mation see the EC Declaration of Conformity document Requirement Content Name and address of GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 manufacturer 84 Uppsala Sweden CE conformity This product complies with the European directives listed in the table below by fulfilling the corresponding harmonized standards For further information see the EC Declaration of Conformity document Directive
15. avant 150 0 01 to 150 ml min Pressure range KTA avant 25 0 to 10 MPa 1450 psi KTA avant 150 0 to 5 MPa 725 psi Valves Viscosity range 0 7 to 10 cP Flow rate AKTA avant 25 specifications e Conditions 0 25 to 25 ml min lt 3 MPa 0 8 to 3 cP e Accuracy 2 e RSD lt 0 5 AKTA avant 150 e Conditions 1 0 to 150 ml min lt 3 MPa 0 8 to 2 cP e Accuracy 2 e RSD lt 05 Parameter Data Type Rotary valves Number of valves Upto 12 Functions Inlet A Inlet B Sample inlet Injection Column pH Outlet KTA avant Installation Guide 29 0351 82 AA 115 10 Reference information 10 2 Component specifications Quaternary valve Parameter Data Type 4 port solenoid actuated membrane valve Functions Quaternary gradients or BufferPro Number of inlets Inlet A 7 expandable to 14 Inlet B 7 expandable to 14 Sample inlet 7 expandable to 14 Quaternary inlet 4 expandable to 18 Pressure sensors Parameter DX Ke Placement of sensors System pump Sample pump Pre Column Post Column Range KTA avant 25 0 to 20 MPa 2900 psi AKTA avant 150 0 to 5 MPa 725 psi Accuracy AKTA avant 25 0 02 MPa or 2 whichever is greater KTA avant 150 0 015 MPa or 1 5 whichever is greater Air sensors Parameter Placement of sensors Inlet A Inlet B Sample inlet 116 AKTA avant I
16. during run Click L Eeee Result Inlet valve B switches to the selected port KTA avant Installation Guide 29 0351 82 AA 67 8 Prime inlets and purge pumps 8 1 Prime buffer inlets and purge System pump A and System pump B Step Action 3 Connect a 25 to 30 ml syringe to the purge valve of the left pump head of System pump B Make sure that the syringe fits tightly into the purge con nector Open the purge valve by turning it counter clockwise about 3 quarters of a turn Draw a small volume of liquid slowly into the syringe with a rate of about 1 ml per second Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents Connect the syringe to the purge valve on the right pump head of System pump B and repeat steps 4 and 5 Keep the system flow running 68 AKTA avant Installation Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 1 Prime buffer inlets and purge System pump A and System pump B Purge System pump A Follow the instructions below to purge both pump heads of System pump A Step Action 1 4 In the Manual instructions dialog e Select Pumps and pressures Gradient e Set Target to 0 B and Length to 0 min Click L_Execute_ Result Only System pump A is active In the Manual instructions dialog e Select Flow path Inlet A e Select the Position of one of the inlets to be used from the drop down list click LE J Resul
17. in the flow path Plastics and rubber materials are selected to avoid leakage of monomers plasticizers or other additives Cleaning chemicals overview Strong cleaning works well with 2 M sodium hydroxide 70 acetic acid or the alcohols methanol ethanol and isopropyl alcohol Complete system cleaning with 1 M hydrochloric acid should be avoided due to sensitivity in the pressure sensors For cleaning separation media with 1 M hydrochloric acid use loop injections of the acid Make sure that the column is not mounted on a column valve V9 C which contains a pressure sensor If sodium hypochlorite is used as sanitizing agent instead of 2 M sodium hydroxide use a concentration up to 10 Chromatography chemicals overview 124 Reversed phase chromatography of proteins works well with 100 acetonitrile and ad ditives trifluoroacetic acid TFA up to 0 2 or formic acid up to 5 Strong organic solvents like ethyl acetate 100 acetone or chlorinated organic solvents should be avoided These might cause swelling of plastic material and reduce the pressure tolerance of PEEK tubing For this reason flash chromatography and straight normal phase is generally not recommended on the system AKTA avant Installation Guide 29 0351 82 AA 10 Reference information 10 4 Chemical resistance guide Assumptions made The ratings are based on the following assumptions e Synergy effects of chemical mixtures have not been taken into
18. inside the Fraction collector 4 Pull out the F shaped piece of foam that holds the Frac arm in position during transportation 5 Attach the Waste funnel in position inside the Frac chamber 44 AKTA avant Installation Guide 29 0351 82 AA 4 Hardware installation 4 1 Unpack the instrument Step Action 6 Press the Button cover onto the button on the Frac arm AKTA avant Installation Guide 29 0351 82 AA 45 4 Hardware installation 4 2 Install the computer equipment 4 2 Install the computer equipment Introduction The computer is either e Supplied as a part of the KTA avant delivery e Supplied locally Unpacking and installing Unpack and install the computer according to the manufacturer s instructions 46 AKTA avant Installation Guide 29 0351 82 AA 4 Hardware installation 4 3 Connect system units 4 3 Connect system units Introduction The following interconnections must be made e Power supply to the KTA avant instrument e Power supply to the computer equipment e Network connection between the computer and the KTA avant instrument WARNING Only use grounded power cords delivered or approved by GE Healthcare WARNING Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before con necting the power cord Connect power to the AKTA avant instrument Follow the instruction below to connect power to
19. lab Dimensioned to accommodate the size and weight of the instrument AKTA avant Installation Guide 29 0351 82 AA 23 3 Site preparation 3 3 Room requirements 3 3 Room requirements Introduction This section describes the requirements for the transportation route and the room where the AKTA avant instrument is placed WARNING Protective ground The AKTA avant instrument must always be connected to a grounded power outlet WARNING Only use grounded power cords delivered or approved by GE Healthcare WARNING Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect WARNING Explosion hazard To avoid building up an explosive atmosphere when using flammable liquids make sure that the room ventilation meets the local requirements Transportation route Doors corridors and elevators must have a minimum width of 65 cm to allow for trans porting the instrument Allow additional space for moving around corners 24 AKTA avant Installation Guide 29 0351 82 AA 3 Site preparation 3 3 Room requirements Space requirements The illustration below shows the space recommended for the AKTA avant system Allow space on the laboratory bench for e handling of samples and buffers 2 x 30 cm e computer and monitor 80 cm e access for service see below A
20. manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense The modules Mixer M9 and UV detector U9 D comply with FCC 47 CFR Part 15c Note This device complies with Part 15c rules Operation is subject to the following two conditions e This device may not cause harmful interference and e This device must accept any interference received including interference that may cause undesired operation The user is cautioned that any changes or modifications not expressly approved by the manufacturer could void the user s authority to operate the equipment Software declaration of conformity UNICORN 6 is technically compatible with all relevant sections of FDA 21 CFR Part 11 A part 11 system assessment checklist is available on request through the local GEHC representative Regulatory compliance of connected equipment Any equipment connected to AKTA avant should meet the safety requirements of EN 61010 1 IEC 61010 1 or other relevant harmonized standards Within EU connected equipment must be CE marked 10 AKTA avant Installation Guide 29 0351 82 AA Environmental Conformity 1 Introduction 1 2 Regulatory information Regulation Title 2011 65 EU Restriction of Hazardous Substances ROHS Directive 2002 96 EC Wast
21. the system pressure curves Air trapped in System pump A Faulty System pump A Air in pumps Make sure to prime inlet tubing A1 and purge System pump A before method start see Section 8 1 Prime buffer inlets and purge System pump A and System pump B on page 63 Faulty pump See User Manual chapter Troubleshooting Liquid is collected in the wrong wells or tubes Incorrect fractionation settings Check the fractionation settings in the System Settings dialog Liquid is collected in wrong Cassette Incorrect preparation of the Fraction collector Make sure that only two Cassettes are placed on the Cassette tray and that the Cassettes are positioned on the cassette positions 1 and 6 Fractionation interrupt ed Impurities on the Dis penser head of the Fraction collector Clean the Fraction collector See User Manual chapter Maintenance Incorrect preparation of buffer and tubing Make sure that the system was correctly prepared see Prepare the test on page 85 AKTA avant Installation Guide 29 0351 82 AA 89 9 Performance tests 9 3 Q valve test 9 3 Qvalve test Method description The Q valve test checks the functionality of the Quaternary valve Correct gradient for mation is tested by producing a series of gradient steps The method run takes approxi mately 30 minutes for AKTA avant 25 and 70 minutes for AKTA avant 150 Required material The following
22. the AKTA avant instrument Step Action 1 Select the correct power cord to be used Each instrument is delivered with 2 alternative power cords e Power cord with US plug 2 m e Power cord with EU plug 2m Discard the unused power cord 2 Connect the power cord to the Power input connector on the back of the instrument and to a grounded wall outlet 100 240 V 50 60 Hz AKTA avant Installation Guide 29 0351 82 AA 47 4 Hardware installation 4 3 Connect system units Step Action 3 Attach the power cord to the rear of the instrument using the cable clip Connect power to computer equipment Follow the manufacturer s instructions to connect power to the e computer e monitor e local printer if used Connect to network Follow the instructions below to make network connections Step Action 1 Connect a network cable between the network connector Ethernet on the back of the instrument and the computer network card marked for AKTA The illustration below shows the symbol of the Ethernet connector 48 AKTA avant Installation Guide 29 0351 82 AA 4 Hardware installatio n 4 3 Connect system units Step Action 2 If the computer is to be connected to an external network connect a network cable between the main network card of the computer and a network wall outlet Note If the computer has not been supplied by GE Healthcare and if network con figuration is to be used see Administration
23. the instruction below to prepare the system before method start Step Action 1 Immerse the pieces of inlet tubing marked A1 B1 and the piece of sample inlet tubing marked Buff in distilled water 2 Prime the inlets A1 B1 and the sample inlet Buff and purge the pumps See Section 8 1 Prime buffer inlets and purge System pump A and System pump B on page 63 and Section 8 2 Prime sample inlets and purge the Sample pump on page 71 3 Disconnect the inlet tubing connected to the inlet valve positions A4 B4 and S4 During the test method air is introduced into the inlet valves through these inlet ports to test the function of the air sensors Start the test Follow the instruction below to start the Air sensor test Step Action 1 In the System Control module select System Performance Test and Report Result The System Performance Test and Report dialog opens 82 AKTA avant Installation Guide 29 0351 82 AA Step Action 9 Performance tests 9 1 Air sensor test 2 In the System Performance Test and Report dialog e Select Air Sensor Test e Click the Run Performance Method button Result The Start Protocol of the Air sensor test opens 3 Click Next in the dialogs of the Start Protocol to open the next dialog The dialogs are described in table Overview of the Start Protocol dialogs on page 83 4 In the last dialog of the Start Protocol Result name and location click Finish Result The Air sensor te
24. to open the next dialog The dialogs are described in table Overview of the Start Protocol on page 98 In the last dialog of the Start Protocol Result name and location click Start Result The System test starts Overview of the Start Protocol The table below describes the dialogs of the Start Protocol Dialog Description Notes Displays the Method Notes of the method The Method Notes contains a method description and instructions on how to run the method This dialog also allows the user to enter Start Notes Evaluation Procedures Allows the user to select to save the report to file recom mended and or to print the report tion Result Name and Loca Allows the user to change result name and result location 98 AKTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 4 System test 9 4 2 Run the test During the run A Message dialog opens during the run Read the messages in the dialog and make sure that necessary preparations have been performed Click the Confirm and Continue button in the Message dialog to change system state from System Pause to Run and proceed with the test Alternatively click the Confirm button in the Message dialog and click the Continue button on the Instrument display Illustration of chromatogram The illustration below shows a chromatogram from a System test 440 430 420 410 400 390 380 370 360 350 340 330 320 310 300 290
25. 7 Holder rails 2 Buffer tray 8 Swivel foot Lock Unlock knob 3 Instrument display 9 Swivel foot 4 Wet side 10 Swing out toolbox 5 Foldable door 11 Power switch 6 Pump cover KTA avant Installation Guide 29 0351 82 AA 13 2 Introduction to KTA avant 2 2 Indicators and controls 2 2 Indicators and controls Introduction This section describes the indicators and controls that are available to the user of the AKTA avant system Illustration The illustration below shows the location of indicators and controls 1 Part Function 1 Power switch 2 Swivel foot Lock Unlock knob 3 Instrument display See description below 14 AKTA avant Installation Guide 29 0351 82 AA Illustration of the Instrument display 2 Introduction to AKTA avant 2 2 Indicators and controls The illustration below shows the Instrument display with the system state Ready showing TRRTRavent j Instrument display indicators and buttons The Instrument display is a touch screen that shows the current system status The In strument display includes the following indicators and buttons Indicator Button Description Indicates if the Instrument display buttons are unlocked or locked The buttons can be locked from UNICORN Sys tem Control Pauses the run and stops all pumps ij i B Resumes instrument operation from the following states e Wash e Pause e Hold
26. AKTA avant 150 has a similar appear ance but a different scale on the y axis Manual evaluation Evaluate the step response 94 Follow the instruction below to calculate the relative absorption plateau heights for curve UV1_ 265 Perform the calculations for each of the three tables in Section 9 5 Test protocol for Q valve test on page 107 Step Action Subtract the baseline value 0 UV from each of the values in column 2 Enter the results in column 3 KTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 3 Q valve test Step Action 2 Divide each value in column 3 by the baseline corrected value corresponding to 100 UV multiply by 100 and enter the results in column 4 3 Check that all values in column 4 fall within the intervals given in column 5 Possible causes of a failed test The table below describes possible causes of a failed test When possible sources of error have been checked and taken care of run the test once again Disturbances caused by air trapped in any of the System pumps Make sure to prime all Q inlets and purge the System pumps before method start see Section 8 3 Prime Q inlets and purge System pump A and System pump B on page 76 Disturbances caused by damaged pump piston seals Replace piston seals See User Manual chapter Mainte nance Unstable or incorrect UV signal or drifting base line Faulty UV monitor See User Manual chapter Tro
27. Action 10 11 12 Connect a 25 30 ml syringe to the outlet tubing of the System pump piston rinsing system Draw liquid slowly into the syringe Disconnect the syringe and discard its contents Immerse the outlet tubing in the rinsing solution tube where the inlet tubing of the System pump piston rinsing system is immersed Connect a 25 30 ml syringe to the outlet tubing from the Sample pump piston rinsing system Draw liquid slowly into the syringe Disconnect the syringe and discard its contents Immerse the outlet tubing in the rinsing solution tube where the inlet tubing of the Sample pump piston rinsing system is immersed Fill the rinsing solution tubes so that each of the tubes contains 50 ml of 20 ethanol AKTA avant Installation Guide 29 0351 82 AA 53 4 Hardware installation 4 4 Install the instrument Location of waste tubing All waste tubing is found on the rear of the instrument see the illustration below Se eeeceeneuassussea See eee ree Part Description 1 Waste tubing from the Injection valve the pH valve and the Outlet valve W W1 W2 and W3 2 Waste tubing from the Fraction collector and the Buffer tray Prepare the waste tubing Follow the instruction below to prepare the waste tubing 54 KTA avant Installation Guide 29 0351 82 AA Step 4 Hardware installation 4 4 Install the instrument Action Place the four pieces of waste tubing from the Inj
28. E logo faces the front of the drawer T Close the Frac drawer Make sure that it snaps into closed position 8 In the System Control module select View Fraction collection content In the Fraction collector content dialog check that the automatic scanning procedures have been performed correctly 9 In the System Control module select System Settings Result The System Settings dialog opens 10 In the System Settings dialog e Select Fraction collector Fractionation settings e For KTA avant 25 In the Fractionation mode drop down list select Accumulator e Inthe Fractionation order drop down list select Row by row AKTA avant Installation Guide 29 0351 82 AA 87 9 Performance tests 9 2 Fraction collector test Start the test Follow the instruction below to start the Fraction collector test Step Action i In the System Control module select System Performance Test and Report Result The System Performance Test and Report dialog opens In the System Performance Test and Report dialog e Select Fraction Collector Test e Click the Run Performance Method button Result The Start Protocol of the Fraction collector test opens Click Next in the dialogs of the Start Protocol to open the next dialog The dialogs are described in table Overview of the Start Protocol dialogs on page 88 In the last dialog of the Start Protocol Result name and location click Finish Result The Fraction collector tes
29. GE Healthcare Life Sciences AKTA avant Installation Guide Original instructions Table of Contents Table of Contents MER OG UCU OA sceicco esiaren anie e a E R 5 1 1 Important user information 6 12 REGQUICEORY INFOMATION sssssssassssssossssssesscorsecenionsnsoseessoosoossacenssvsnsvosaassseesseensnrsbsssunsssebedenisevvssiastisteaneeny 8 2 Introduction to AKTA OVANT sosisini esi rn aiana 12 2 1 The KTA avont instrument secesssssssssssssenssssssssssessssnsseusnsssstunnnsssesssnssnuestesentnnesesnssasesesseeeeee 13 22 indicators AME Control S eninin a a RUNAS 14 2 53 CONNECTS mnssiiusnnnn iea a aA EET a 17 24 Network architectUr emisiei i AE 18 Bi Site preparati sicsecesseececscsecsssectecksss di savecscsscsciscsciecaccesiaeacsuestcseiadessestessizedhssestestiss 20 3U INtODUCHOM aenn a AN EANA ia ates 21 3 2 Delivery and storage 22 3 3 Room FEQUIFERMEMIS sionister aiai aniraa Esnas aaas i 24 34 Site enVironNMEeN sussista ansesasansastacaastssstesosasanaete 28 3 5 Power requirements 29 3 6 Computer requirements 31 37 R g ired TAGS RIGS ssscdsscscstessasesssssssscsceteneste vows tsvsossacdecevstvansssuaavaiosanncoesosbgnbsbadnsanatiaesduncabionvctaveendebiets 32 G Hardware installati n cc cccscsssscsssesseesteessecssscsvesesessssecsesessesecsossssetbesesessoseestenevee 35 4 1 Unpack the ISERIES TUE sirisser aana aT iei 36 4 2 Install the computer equipment u ecssssssse
30. ING Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before con necting the power cord gt PP Requirements The table below specifies the power requirements Parameter Requirement Supply voltage 100 240 V Frequency 50 60 Hz Max power consump 800 VA tion AKTA avant Installation Guide 29 0351 82 AA 29 3 Site preparation 3 5 Power requirements Parameter Requirement Number of sockets 1 socket per instrument up to 3 sockets for computer equipment Type of sockets EU or US plugs Grounded mains sockets fused or protect ed by equivalent circuit breaker Location of sockets Maximum 2 m from the instrument due to length of mains cable Extension cables can be used if required Quality of power The mains power supply must be stable and conform to specifications at all times to ensure reliable operation of AKTA avant There should be no transient or slow changes in average voltage outside the limits specified above 30 AKTA avant Installation Guide 29 0351 82 AA 3 Site preparation 3 6 Computer requirements 3 6 Computer requirements Introduction KTA avant systems are controlled by UNICORN software running on a PC The PC can be part of the delivery or be supplied locally The PC used must fulfill the recommendations stated in this section Computer specifications Refer to UNICO
31. KTA avant Installation Guide 29 0351 82 AA 25 3 Site preparation 3 3 Room requirements Service access To access the rear panel the instrument can be rotated on a swivel foot There must be at least 20 cm additional space on the bench to allow for free rotation WARNING Rotating the instrument Make sure that there is always at least 20 cm of free space around the AKTA avant instrument to allow for sufficient ventilation and rotation on the swivel foot When ro tating the instrument take care not to stretch or squeeze tubing or cables A disconnected cable may cause power interruption or network interruption Stretched tubing may cause bottles to fall resulting in liquid spillage and shattered glass Squeezed tubing may cause increase in pressure or block liquid flow To avoid the risk of knocking over bottles always place bottles on the buffer tray and close the doors before rotating the instrument Laboratory bench The bench must be clean flat and stable to support the weight of the AKTA avant system see table Equipment weight below 26 AKTA avant Installation Guide 29 0351 82 AA 3 Site preparation 3 3 Room requirements Equipment dimensions The outer dimensions of the AKTA avant instrument are shown in the illustration below 66cm Equipment weight Item Weight AKTA avant instrument 116 kg Computer 9 kg Monitor 3 kg Total 128 kg AKTA avant Installation Guide
32. Protocol of the test the report is automatically printed on the system printer Note that a system printer must have been set up see UNICORN Administration and Technical Manual Print the report and check the status of the tests For each of the tests the report states The test passed or The test failed Manual evaluation Preparation 100 If you prefer to evaluate your results manually follow the instructions below Step Action il In the Evaluation module in UNICORN click the Open Result Navigator icon n Result The Result Navigator opens 2 In the Result Navigator double click the test result name to open the test result 3 Click the Customize icon W4 Result The Customize dialog opens 4 In the Customize dialog select the Curves tab KTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 4 System test 9 4 3 Evaluate the test Step Action 5 In the Curves tab e Select the following curves to be displayed UV1_265 UV2_254 UV3_280 Cond ConcB e Click OK 6 In the Chromatogram pane of the Evaluation module e Select the curve UV1_ 265 e Right click and select Vertical marker 7 Click the vertical marker and drag it to the constant section of each plateau of the curve UV1_265 Read the absorbance values of each plateau in the upper left corner of the Chromatogram pane and enter the values in the column 2 in table Step response test result on page 109 8 Read t
33. RN Administration and Technical Manual for computer specifications AKTA avant Installation Guide 29 0351 82 AA 31 3 Site preparation 3 7 Required materials 3 7 Required materials Introduction This section describes the accessories required for the installation and operation of AKTA avant Buffers and solutions The buffers and solutions listed below are required during the installation procedure and should be provided at the installation site Buffer solution Requiredvolume Scope of use Distilled water 1 liter Air sensor test Fraction collector test Q valve test System test 1 acetone in distilled 0 5 liter Q valve test water 1 acetone and 1M NaCl 0 5 liter System test in distilled water 20 ethanol 200 ml Priming of the pump piston rinsing system Laboratory equipment The equipment listed below is required during the installation procedure and should be provided at the installation site Flasks liquid containers For buffers and waste Gloves For protection Protective glasses For protection 32 KTA avant Installation Guide 29 0351 82 AA 3 Site preparation 3 7 Required materials Fraction collector tubes The tubes used in the Fraction collector of AKTA avant must fulfill the requirements listed in the table below Examples of manufacturers are also listed in the table Tube size Diameter mm Height mm Max Examples of ml volume manufact
34. System pump B 76 AKTA avant Installation Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 1 Prime buffer inlets and purge System pump A and System pump B 8 1 Prime buffer inlets and purge System pump A and System pump B Overview The procedure consists of the following stages Prime all inlet tubing B to be used during the run Prime all inlet tubing A to be used during the run Prepare the system before purging the System pumps Purge System pump B Purge System pump A Dn HO FP WN N e End the run Prime inlet tubing B Follow the instructions below to fill all B inlet tubing to be used in the run with appropriate buffer solution Step Action 1 Make sure thot all B inlet tubing that is to be used during the method run is immersed in the correct buffers KTA avant Installation Guide 29 0351 82 AA 63 8 Prime inlets and purge pumps 8 1 Prime buffer inlets and purge System pump A and System pump B Step Action Z In the Manual instructions dialog e Select Flow path Inlet B e Select the Position of the inlet to be filled from the drop down list Start at the inlet position with the highest number and end at the inlet position with the lowest number Manual instructions System3 Selected column type Select Instructions i Parameters for Inlet B 4 Pumps and pressures Rad Position Flow path i Injection valve B1 S Column position Inlet PH valve Sample inlet
35. Test protocol for System test Gradient test result Gradient linear from W B tO ansni B The gradient must be linear from 5 to 90 with an allowed deviation of 1 00 Step response test result 1 2 3 4 5 Programmed Valueread mAU Baseline corrected Normalized value Allowed interval Conc B value 100 7 95 94 00 96 00 50 49 00 51 00 5 4 00 6 00 o 2 UV response test result 1 2 3 4 5 Wavelength nm Value read mAU Baseline corrected Normalized Allowed interval value veie 100 B 0 B 254 z 265 254 1 10 1 23 265 265 280 5 1 30 1 58 280 a KTA avant Installation Guide 29 0351 82 AA 109 9 Performance tests 9 6 Test protocol for System test Sample peak and conductivity test result 3 4 Area Peak area Allowed interval 1 65 00 67 00 2 33 00 35 00 110 AKTA avant Installation Guide 29 0351 82 AA 10 Reference information 10 Reference information About this chapter This chapter lists the technical specifications of the AKTA avant The chapter also includes a list of wetted materials and a chemical resistance guide In this chapter This chapter contains the following sections Section See page 10 1 System specifications 112 10 2 Component specifications 114 10 3 Wetted materials 121 10 4 Chemical resistance guide 124 AKTA avant Insta
36. are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information UNICORN Any use of this software is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products A copy of this Standard Software End User License Agreement is available on request GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 29 0351 82 AA 03 2013
37. at 0 to 2 AU Operating pressure 0 to 2 MPa 290 psi Flow cells e 0 5mm optical path length 1 ul cell volume e 2 mm optical path length 2 ul cell volume e 10mm optical path length 8 pl cell volume 118 AKTA avant Installation Guide 29 0351 82 AA 10 Reference information 10 2 Component specifications Conductivity monitor Parameter Data Conductivity reading range 0 01 MS cm to 999 99 mS cm Accuracy 0 01 mS cm or 2 whichever is greater within 0 3 to 300 mS cm Operating pressure 0 to 5 MPa 725 psi Flow cell volume 22 ul Temperature monitor Parameter Reading range 0 C to 99 C Accuracy 1 5 C within 4 C to 45 C pH monitor Parameter Data pH reading range Oto 14 Accuracy 0 1 pH unit within pH 2 to 12 temp within 3 C from calibra tion temp Operating pressure 0 to 0 5 MPa 72 psi Flow cell volume 76 ul Fraction collector Parameter Number of fractions up to 576 AKTA avant Installation Guide 29 0351 82 AA 119 10 Reference information 10 2 Component specifications Parameter Data Vessel types 3 8 15 or 50 ml tubes 250 ml bottles Deep well plates 96 48 24 Vessel type selection Automatic recognition Fraction volumes 0 1 to 50 ml Spillage free modes AKTA avant 25 Drop sync or Accumulator AKTA avant 150 Accumulator Protection of fractions Covered vessels and temperature control Organ
38. cess the instrument handles 40 AKTA avant Installation Guide 29 0351 82 AA 4 Hardware installation 4 1 Unpack the instrument Step Action 7 Grip the handles on the front and wet side of the instrument On the back and left hand side grip the metal plates located at the bottom of the instru ment AKTA avant Installation Guide 29 0351 82 AA 41 4 Hardware installation 4 1 Unpack the instrument Step Action 8 Lift the instrument and at the same time pull away the plywood board from under the instrument Accessories packages The illustration below shows the accessories packages placed in the Buffer tray at delivery 42 AKTA avant Installation Guide 29 0351 82 AA 4 Hardware installation 4 1 Unpack the instrument Part Description 1 Cassette tray 2 Manual box 3 Fraction collector cassettes 4 pH electrode 5 Accessories box Remove transport fixations from inside the Fraction collector Follow the instruction below to remove the transport fixations from inside the Fraction collector Step Action al Pull out the deep well plates the Waste funnel and the Button cover from the holes in the piece of foam located in the Frac chamber 2 Fold forward the vertical section of the foam and fold over the right edge of the foam KTA avant Installation Guide 29 0351 82 AA 43 4 Hardware installation 4 1 Unpack the instrument Step Action 3 Pull out the foam from
39. ct to system 7 Start UNICORN and connect to sys tem Introduction This section describes how to start and log on to UNICORN and how to connect the in strument to UNICORN Start UNICORN and log on Follow the instructions below to start UNICORN and log on to the program Step Action al Double click the UNICORN icon on the desktop Result The Log On dialog opens Note If there is no connection to the database it is still possible to log on to UNICORN and control a running system The Log On dialog will give the option to start System Control without a database Click Start System Control to proceed to the next Log On dialog AKTA avant Installation Guide 29 0351 82 AA 59 7 Start UNICORN and connect to system Step Action 2 In the Log On dialog e select User Name and e enter Password Note It is also possible to select the Use Windows Authentication checkbox and enter a network ID in the User Name field F Log On UNICORN O Use Windows Authentication User Name Password Domain Access Group Eric Administrators od e click the Options button and select which UNICORN modules to start Start UNICORN Administration C System Control Method Editor O Evaluation e click OK Result The selected UNICORN modules open 7 Administration D t4 Ig f User P SEA unice i gt System Control amp Method Editor Conn
40. describes how to start the instrument and the computer Instruction Follow the instructions below to start the instrument and the computer Step Action 1 Switch on the instrument by pressing the Power switch to the I position Result The instrument starts and the Instrument display states Not connect ed 2 Turn on the computer and monitor according to the manufacturer s instruc tions AKTA avant Installation Guide 29 0351 82 AA 57 6 Software installation 6 Software installation Introduction This section gives an overview of the different UNICORN installation types Detailed information about software installation and configuration is available in the UNICORN Administration and Technical Manual Software installations UNICORN can be installed as listed below a complete UNICORN installation on a stand alone workstation Full installation a UNICORN database and license server Custom installation and UNICORN software client and instrument server software on a network client station Custom installation It is also possible to define a system as part of the installation configure E licenses configure Windows settings necessary for the UNICORN process pictures in a network deployment configure firewall settings when necessary upgrade UNICORN remove UNICORN installations and set up a system printer 58 AKTA avant Installation Guide 29 0351 82 AA 7 Start UNICORN and conne
41. e Disconnect the syringe and discard its contents 6 Repeat steps 3 5 for the other three purge valves of the System pumps to get rid of air in all pump heads Keep the system flow running End the run Click the End icon in the System Control toolbar to end the run L 80 KTA avant Installation Guide 29 0351 82 AA 9 Performance tests About this chapter 9 Performance tests Performance tests should be run after installation to check the function of the AKTA avant system Performance tests can also be used at any time to check the condition of the system for example after a prolonged stop This chapter describes how to prepare run and evaluate the four performance tests available In this chapter This chapter contains the following sections Section See page 9 1 Air sensor test 82 9 2 Fraction collector test 85 9 3 Q valve test 90 9 4 System test 96 9 5 Test protocol for Q valve test 107 9 6 Test protocol for System test 109 81 AKTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 1 Air sensor test 9 1 Air sensor test Method description The Air sensors A B and S are integrated in Inlet valve A Inlet valve B and Sample inlet valve The Air sensor test checks if the air sensors detect air The method run takes ap proximately 5 minutes Required material The following material is required e Syringe 25 30 ml e Distilled water Prepare the test Follow
42. e Electrical and Electronic Equipment WEEE Direc tive Regulation EC No 1907 2006 Registration Evaluation Authorization and restriction of CHemicals REACH ACPEIP AKTA avant Installation Guide 29 0351 82 AA Administration on the Control of Pollution Caused by Electronic Information Products China Restriction of Hazardous Substances RoHS 11 2 Introduction to AKTA avant 2 _ ntroduction to AKTA avant About this chapter The AKTA avant system comprises the AKTA avant instrument the UNICORN software and accessories This chapter gives an overview of the AKTA avant system and describes the location of indicators and controls on the instrument Illustration of the system The illustration below shows the AKTA avant instrument with UNICORN software installed on a computer In this chapter This chapter contains the following sections Section See page 2 1 The AKTA avant instrument 13 2 2 Indicators and controls 14 2 3 Connectors 17 2 4 Network architecture 18 12 AKTA avant Installation Guide 29 0351 82 AA 2 Introduction to AKTA avant 2 1 The AKTA avant instrument 2 1 The KTA avant instrument Introduction This section provides an overview of the AKTA avant instrument Illustration of the main parts of the instrument The illustration below shows the location of the main parts of the instrument Part Function Part Function 1 Fraction collector
43. e data needed to prepare the laboratory for the installation In this chapter This chapter contains the following sections Section See page 3 1 Introduction 21 3 2 Delivery and storage 22 3 3 Room requirements 24 3 4 Site environment 28 3 5 Power requirements 29 3 6 Computer requirements 31 3 7 Required materials 32 20 AKTA avant Installation Guide 29 0351 82 AA 3 Site preparation 3 1 Introduction 3 1 Introduction Why site preparation The laboratory site must be planned and prepared before installing the KTA avant system The performance specifications of the system can be met only if the laboratory environment fulfills the requirements stated in this chapter The time spent in preparing the laboratory will contribute to the long term performance of the systems Required knowledge Personnel that will install the system must have e Ageneral understanding of how a PC and Microsoft Windows operating system work In most cases universal computer functions will not be explained e Anunderstanding of the concepts of liquid chromatography Terminology and functions will be explained only when they differ from normal practice e Ageneral understanding of risks associated with handling chemicals and substances for use in liquid chromatography AKTA avant Installation Guide 29 0351 82 AA 21 3 Site preparation 3 2 Delivery and storage 3 2 Delivery and storage Introduction This section describes
44. e network configurations e Workstation configuration local network and a local database e Network configuration distributed network using TCP IP and a central database Office computers can be used to set up runs view and evaluate run data In this guide only the workstation configuration is described For further information about network configuration please refer to UNICORN Administration and Technical Manual Illustration Workstation configuration The illustration below shows a typical workstation configuration Workstation computer License server 2D Barcode scanner Database UNICORN Instrument 18 KTA avant Installation Guide 29 0351 82 AA 2 Introduction to AKTA avant 2 4 Network architecture Illustration Network configuration The illustration below shows a possible network configuration Office computer UNICORN Database server 2D Barcode scanner Database License server Instrument server 2D Barcode scanner Note Griffin is a software tool that can only be used by GE Healthcare service staff This service tool is used for diagnostic testing and quality control AKTA avant Installation Guide 29 0351 82 AA 19 3 Site preparation 3 Site preparation About this chapter This chapter describes the site planning and the preparations necessary to perform before installation of an AKTA avant system The purpose is to provide planners and technical staff with th
45. ect to system Follow the instructions below to connect the instrument to UNICORN 60 AKTA avant Installation Guide 29 0351 82 AA 7 Start UNICORN and connect to system Step Action al In the System Control module click the Connect to Systems icon P EA Result The Connect to Systems dialog opens Connect to Systems Connected systems 1 selected max 3 System name Control View E System E System2 g E System3 E Systems E B Systems E Systems E System 2 In the Connect to Systems dialog e Select a system e Select Control mode e Click OK Result The selected instrument can now be controlled by the software KTA avant Installation Guide 29 0351 82 AA 61 8 Prime inlets and purge pumps 8 Prime inlets and purge pumps About this chapter Before starting the performance tests described in Chapter 9 Performance tests on page 81 itis important to 1 Prime the inlets fill the inlets with liquid 2 Purge the pumps remove air from the pumps This chapter describes how to prime the buffer inlets sample inlets and Q inlets and how to purge the System pumps and the Sample pump In this chapter 62 This chapter contains the following sections Section See page 8 1 Prime buffer inlets and purge System pump A and System pump B 63 8 2 Prime sample inlets and purge the Sample pump 71 8 3 Prime Q inlets and purge System pump A and
46. ection valve the pH valve and the Outlet valve W W1 W2 and W3 in a vessel placed below the bench NOTICE gt The maximum level of the waste vessel for the waste tubing from the valves must be lower than 30 cm above the lab bench Place the three pieces of waste tubing from the Fraction collector and the Buffer tray in a waste vessel placed below the bench NOTICE gt The maximum level of the waste vessel for the waste tubing from the Fraction collector and the Buffer tray must be lower than the bench height Cut the waste tubing from the Fraction collector and the Buffer tray to ap propriate length It is important that the tubing is not bent and will not be submerged in liquid during the run q Note Ifthe tubing is too short replace it with new tubing Do not lengthen the tubing as this might cause obstruction of the tubing and flooding in the Frac chamber KTA avant Installation Guide 29 0351 82 AA 55 4 Hardware installation 4 4 Install the instrument CAUTION Make sure that the waste vessels will hold all the produced volume of the run For AKTA avant 25 a suitable waste vessel should typi cally have a volume of 2 to 10 liters For AKTA avant 150 a waste vessel should have a volume of 40 liters 56 AKTA avant Installation Guide 29 0351 82 AA 5 Start the instrument and the computer 5 Start the instrument and the com puter Introduction This section
47. em pump A Make sure that the syringe fits tightly into the purge con nector 4 Open the purge valve by turning it counter clockwise about 3 quarters of a turn Draw liquid slowly into the syringe until the liquid reaches the pump 5 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 6 Repeat steps 2 5 for each piece of inlet tubing A that is to be used during the run AKTA avant Installation Guide 29 0351 82 AA 65 8 Prime inlets and purge pumps 8 1 Prime buffer inlets and purge System pump A and System pump B Prepare the system before purging the System pump heads Follow the instructions below to prepare the system Step Action il Make sure that the piece of waste tubing connected to Injection valve port W1 is placed in a waste vessel 2 Open the System Control module and select Manual Execute Manual In structions Result The Manual instructions dialog opens 3 In the Manual instructions dialog e Select Flow path Injection valve and select System pump waste from the Position drop down list Click Execute Result The Injection valve switches to waste position This is necessary to achieve a low backpressure during the purge procedure e Select Pumps and pressures System flow For KTA avant 25 set the Flow rate to 1 0 ml min For KTA avant 150 set the Flow rate to 10 0 ml min Click Execute Result A system flow starts 66 AKTA avant Installat
48. ems The illustration below shows the tubing configuration of the pump piston rinsing systems x w 7 Part Description 1 Inlet tubing to the Sample pump piston rinsing system 2 Outlet tubing from the Sample pump piston rinsing system 3 Inlet tubing to the System pump piston rinsing system 4 Outlet tubing from the System pump piston rinsing system KTA avant Installation Guide 29 0351 82 AA 51 4 Hardware installation 4 4 Install the instrument Prime the pump piston rinsing system Follow the instruction below to fill the pump piston rinsing systems with rinsing solution See the tubing configuration of the rinsing systems in Illustration of the pump piston rinsing systems on page 51 Step Action 1 Unscrew the rinsing system tubes from the holders 2 Fill each of the rinsing system tubes with 50 ml of 20 ethanol 3 Screw the rinsing solution tubes back in the holders 4 Immerse the inlet tubing to the System pump piston rinsing system in one of the rinsing solution tubes Note Make sure that the inlet tubing reaches to the very bottom of the rinsing solu tion tube 5 Immerse the inlet tubing to the Sample pump piston rinsing system in the other rinsing solution tube Note Make sure that the inlet tubing reaches to the very bottom of the rinsing solu tion tube 52 KTA avant Installation Guide 29 0351 82 AA Step 4 Hardware installation 4 4 Install the instrument
49. essesscsscsssssssssssesesssssccssssessssusssssseccesssssssuuuecseseceesssesssees 46 4 3 Connect system UNITS sisii innii E a ANAE AA AR R 47 44 DVS tll tS AS TUS iit nensi TO AAS E 50 5 Start the instrument and the computer csseseseseececscecsesececececeeseceeseeeeees 57 G Software ImStell ation secsssscesccszsctarsizecciceccsvssestaaviasecievedaceaducncstevadtideeasatiaaseeseusttacs 58 7 Start UNICORN and connect to system ssssessesssessessssessssessssseesesessessesesesses 59 8 Prime inlets and purge pUmMpS s sssssssssessssesssssesssssessesssseessseseesessesessessenssessesesses 62 8 1 Prime buffer inlets and purge System pump A and System pump B 63 8 2 Prime sample inlets and purge the Sample PUMP uu eessssssssssessesssssscssssssssseetsessccessssssssesnees 71 8 3 Prime Q inlets and purge System pump A and System PUMP B n sssssssssss151s1150111511112 76 9 Performance Tests esiseisoisistireissi rei eieiei sisena areia iaie a R RTE REESS Tki SERT RiS 81 Od ASENSO TEST nonannannannn na nn EAAS R n RA 82 9 2 Fraction col ctort St minamina n ANANN ANT 85 9 3 Q valve test 90 9 4 System test 96 QSL Preparethe test sax sasess seccarcssassa chest naa AEAN A EAA AET EAN 97 DAD E a E ACS AETA EENES E E 98 943 Ev lyate the LOSE sssini ieni e RN 100 KTA avant Installation Guide 29 0351 82 AA 3 Table of Contents 10 9 4 4 Possible causes of a failed test scsssssccssssssisssssenetcocishsseettiendictan
50. ethod start Step Action 1 2 Immerse the piece of inlet tubing marked A1 in distilled water Immerse the piece of inlet tubing marked B1 in 1 acetone and 1 M NaCl in distilled water Immerse the piece of inlet tubing marked Buff in 1 acetone and 1 M NaCl in distilled water Connect the capillary loop marked Ref 1 between Column valve ports 1A and 1B to generate a backpressure For AKTA avant 25 Make sure that the Mixer with a chamber volume of 1 4 ml is installed For AKTA avant 150 Make sure that the Mixer with a chamber volume of 5 ml is installed For further information see User Manual chapter Maintenance Prime the buffer inlets and purge System pump A and System pump B See Section 8 1 Prime buffer inlets and purge System pump A and System pump B on page 63 and Section 8 2 Prime sample inlets and purge the Sample pump on page 71 AKTA avant Installation Guide 29 0351 82 AA 97 9 Performance tests 9 4 System test 9 4 2 Run the test 9 4 2 Run the test Start the test Follow the instruction below to start the System test Step Action 1 In the System Control module select System Performance Test and Report Result The System Performance Test and Report dialog opens In the System Performance Test and Report dialog e Select System Test e Click the Run Performance Method button Result The Start Protocol of the System test opens Click Next in the dialogs of the Start Protocol
51. ft the instrument onto the bench using the overhead crane 5 Disconnect the safety hooks of the straps from the lifting yokes of the ply wood board Lift the instrument onto the bench by hand If an overhead crane is not available lift off the cardboard hood and disconnect the safety hooks of the straps from the lifting yokes of the plywood board under the instru ment Lift the instrument by hand using the handles illustrated in steps 6 7 in instruction Remove transport fixations on page 39 38 AKTA avant Installation Guide 29 0351 82 AA 4 Hardware installation 4 1 Unpack the instrument Remove transport fixations Follow the instruction below to remove the transport fixations from the instrument Step Action 1 2 Lift off the plywood board from the top of the instrument Remove the pieces of foam from around the instrument Check the contents in the Buffer tray according to the illustration Accessories packages on page 42 and lift off the packages from the tray Unscrew the screws from the Buffer tray securing plate and remove the plate Fasten the screws back into place AKTA avant Installation Guide 29 0351 82 AA 39 4 Hardware installation 4 1 Unpack the instrument Step Action 5 Remove the pieces of adhesive tape placed on the locations marked in the illustrations below 6 Open the pump cover and lift off the tray on the wet side and the tray on the front of the instrument to ac
52. he absorbance values for the plateaus corresponding to 0 B and 100 B for the curves listed below Click the curve name in Chromatogram pane to change the curve reading e UV1_265 e UV2_254 e UV3_280 Enter the absorbance values in column 2 in table UV response test result on page 109 AKTA avant Installation Guide 29 0351 82 AA 101 9 Performance tests 9 4 System test 9 4 3 Evaluate the test Step Action 9 Click the Print icon Result The Print Chromatograms dialog opens Print Chromatograms Printer Microsoft Office Document Image Writer aN Properties Print Format m Lo BE U B E Chromatograms in each column Chromatograms in each row Use thick lines 10 In the Print Chromatograms dialog e Select Printer e Click OK Result Your chromatogram is printed Manual evaluation Evaluate the gradient Follow the instruction below to evaluate the gradient for curve UV1_265 Step Action 1 Place a ruler along the gradient part of curve UV1_265 in the printed report 2 Check that the curve is linear between 5 B and 90 B and void of discon tinuities 3 Enter the start and stop values of the linear section of the curve in Gradient test result on page 109 102 KTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 4 System test 9 4 3 Evaluate the test Manual evaluation Evaluate the step response Follow the instruction below to calculate the re
53. ic solvents Ei Delay volume UV Dispenser KTA avant 25 518 yl head KTA avant 150 1831 ul Temperature control Target temperature 6 C to 20 C 120 KTA avant Installation Guide 29 0351 82 AA 10 Reference information 10 3 Wetted materials 10 3 Wetted materials Introduction This section specifies the wetted materials of AKTA avant Wetted materials The following wetted materials are used in AKTA avant Material Component Aluminium oxide Sample pump Borosilicate Fraction collector ECTFE EthyleneChloroTriFluoroEthylene System pumps Sample pump ETFE EthyleneTetraFluoroEthylene Tubing EPDM EthylenePropyleneDiene M class rubber Quaternary valve Fraction collector Elgiloy UHMWPE UltraHighMolecularWeightPolyEthylene System pumps FullyFluorinatedPropyleneMonomer Sample pump FEP Tubing FluorinatedEthylenePropylene FFPM FFKM pH valve Flow restrictor Pressure monitors FPM FKM FluorinatedPropyleneMonomer Mixer chamber Sample pump Hastelloy C 276 Pressure monitors PCTFE PolyChloroTriFluoroEthylene Conductivity monitor KTA avant Installation Guide 29 0351 82 AA 121 10 Reference information 10 3 Wetted materials Material Component PEEK Quaternary valve PolyEtherEtherKetone Inlet valves System pumps Pressure monitors Mixer chamber Injection valve Column valve UV flow ce
54. ion Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 1 Prime buffer inlets and purge System pump A and System pump B Purge System pump B Follow the instruction below to purge both pump heads of System pump B Step Action 1 In the Manual instructions dialog e Select Pumps and pressures Gradient e Set Target to 100 B and Length to 0 min Manual instructions System3 Selected column type l _ Select Instructions 7 Parameters for Gradient Pumps and pressures a Target 0 0 100 0 System flow SSS Sem lias Sample flow L 100 0 8 P h Length 0 00 100000 0 ump wasl a a System wash ooo oo min Quatemary start concentrations Quatemary gradient BufferPro pH F Column packing flow amp Flow path Monitors v Save resultas Browse 2 V Auto update of parameters during run tick Ete Click L_Ewecute_ Result Only System pump B is active 2 In the Manual instructions dialog e Select Flow path inlet B e Select the Position of one of the inlets to be used from the drop down list Manual instructions System3 Selected column type lL Select Parameters for Inlet B Pumps and pressures a Position Flow path Injection valve Bt Mi Column position Inlet A pH valve Sample inlet Outlet valve Injection mark Monitors Fraction collection Instructions lt Save result as __ Browse 2 V Auto update of parameters
55. ion Save the report to file was selected in the Evaluation Procedures dialog in the Start Protocol of the test the report is saved in location C Program Files GE Healthcare UNICORN Temp Open the report and print it using your default Windows printer Recommended Note The exact search path depends on the location of the UNICORN installation folder e Ifthe option Print report was selected in the Evaluation Procedures dialog in the Start Protocol of the test the report is automatically printed on the system printer Note that a system printer must have been set up see UNICORN Administration and Technical Manual Print the report and check the status of the tests For each of the tests the report states The test passed or The test failed Manual evaluation Preparation 92 If you prefer to evaluate your results manually follow the instructions below Step Action 1 In the Evaluation module in UNICORN click the Open Result Navigator icon Result The Result Navigator opens 2 In the Result Navigator double click the test result name to open the test result KTA avant Installation Guide 29 0351 82 AA Step Action 9 Performance tests 9 3 Q valve test 3 In the Chromatogram pane of the Evaluation module e Select the curve UV1_265 e Right click and select Vertical marker 4 Click the vertical marker and drag it to the constant section of each plateau of the curve UV1_265 Read the cor
56. lative absorption plateau heights for curve UV1_ 265 Step Action 1 Subtract the baseline value 0 B from each of the values in column 2 in table Step response test result on page 109 Enter the results in column 3 2 Divide each value in column 3 by the baseline corrected value corresponding to 100 B multiply by 100 and enter the results in column 4 3 Check that all values in column 4 fall within the intervals given in column 5 Manual evaluation Evaluate the UV response Follow the instruction below to calculate the UV response Step Action 1 Subtract the baseline values 0 B corresponding to each UV curve from the values corresponding to 100 B in column 2 in table UV response test result on page 109 Enter the results in column 3 2 Calculate the absorbance ratios 265 254 nm and 265 280 nm using the values in column 3 Enter the values in column 4 3 Check that the obtained ratios fall within the intervals given in column 5 Manual evaluation Evaluate the Sample Peak and Conductivity Test Result Follow the instruction below to inspect the two sample peaks of the Cond curve in the chromatogram Step Action 1 Check that the Cond curve has a shape similar to the shape of the UV curves KTA avant Installation Guide 29 0351 82 AA 103 9 Performance tests 9 4 System test 9 4 3 Evaluate the test Step Action 2 Check that the baseline of the Cond curve looks correct and is not drifting
57. ll Conductivity monitor pH valve Flow restrictor Sample pump Outlet valve Fraction collector Tubing PolyEthylene Fraction collector Polyimide UV flow cell PP Mixer chamber PolyPropylen Fraction collector PPS Fraction collector PolyPhenyleneSulfide PTFE Mixer chamber PolyTetraFluorEthylene Injection valve Column valve UV flow cell pH valve Outlet valve PVDF System pumps PolyVinylideneDiFluoride Sample pump Ruby System pumps Sample pump 122 AKTA avant Installation Guide 29 0351 82 AA 10 Reference information 10 3 Wetted materials Material Component Sapphire System pumps Sample pump Silica UV flow cell Silicone Tubing Titanium System pumps Pressure monitors Conductivity monitor Sample pump AKTA avant Installation Guide 29 0351 82 AA 123 10 Reference information 10 4 Chemical resistance guide 10 4 Chemical resistance guide Introduction This section specifies the chemical resistance of AKTA avant to some of the most com monly used chemicals in liquid chromatography Biocompatibility AKTA avant is designed for maximum biocompatibility with biochemically inert flow paths constructed mainly from titanium PEEK and highly resistant fluoropolymers and fluoroelastomers Titanium is used as far as possible to minimize contribution of poten tially deactivating metal ions such as iron nickel and chromium There is no standard stainless steel
58. llation Guide 29 0351 82 AA 111 10 Reference information 10 1 System specifications 10 1 System specifications Introduction This section lists the system specification data of AKTA avant For components data see Section 10 2 Component specifications on page 114 System specifications Parameter Data System configuration Benchtop system external computer Control system For AKTA avant 25 UNICORN 6 0 or higher version For AKTA avant 150 UNICORN 6 1 or higher version Connection between PC and instrument Ethernet Dimensions W x D x H 860 x 710 x 660 mm Weight excluding computer 116 kg Power supply 100 240 V 50 60 Hz Power consumption 800 VA Enclosure protective class IP 21 wet side IP 22 112 AKTA avant Installation Guide 29 0351 82 AA 10 Reference information 10 1 System specifications Parameter Data Tubing and connectors AKTA avant 25 e Inlet FEP tubing i d 1 6 mm Tubing connector 5 16 Ferrule yellow 1 8 e Pump to Injection valve PEEK tubing i d 0 75 mm Fingertight connector 1 16 e After Injection valve PEEK tubing i d 0 50 mm Fingertight connector 1 16 AKTA avant 150 e Inlet FEP tubing i d 2 9 mm Tubing connector 5 16 Ferrule blue 3 16 e After pumps PEEK tubing 1 0 mm i d Fingertight connector 1 16 Environmental ranges Parameter Data Storage and transport temperature range 25 C to
59. lty UV monitor See User Manual chapter Troubleshooting Wrong Mixer chamber size or faulty Mixer Replace the Mixer See User Manual chapter Maintenance Faulty Step Response Result If all values are faulty air in the pump or a faulty pump Air in pumps Make sure to prime the buffer inlets and to purge the System pumps and the Sample pump before method start See Section 8 1 Prime buffer inlets and purge System pump A and System pump B on page 63 and Section 8 2 Prime sample inlets and purge the Sample pump on page 71 Faulty pump See User Manual chapter Troubleshooting Faulty values at 5 damaged pump piston seal in System pump B Replace piston seals See User Manual chapter Mainte nance AKTA avant Installation Guide 29 0351 82 AA 105 9 Performance tests 9 4 System test 9 4 4 Possible causes of a failed test Faulty values at 95 Replace piston seals See User Manual chapter Mainte damaged pump piston nance seal in System pump A Faulty UV Response Test Result Faulty UV monitor Restart the instrument to calibrate the UV monitor See User Manual chapter Troubleshooting Faulty Runlog Event Test Result Cause Action Any of the valves do not See User Manual chapter Troubleshooting switch Faulty Sample Peak and Conductivity Test Result If the shape of the Cond See User Manual chapter Troubleshooting curve differs from the shape of the UV c
60. material is required e 1 acetone in distilled water e Distilled water e Capillary loop Ref 1 e Syringe 25 30 ml e For KTA avant 25 Mixer 1 4 ml e For KTA avant 150 Mixer 5 ml Prepare the test Follow the instructions below to prepare the system before method start Step Action 1 Immerse the pieces of inlet tubing marked Q1 and Q3 in 1 acetone in dis tilled water 2 Immerse the pieces of inlet tubing marked Q2 and Q4 in distilled water 3 Connect the capillary loop marked Ref 1 between Column valve ports 1A and 1B to generate a backpressure 4 For KTA avant 25 Make sure that the Mixer with a chamber volume of 1 4 ml is installed For KTA avant 150 Make sure that the Mixer with a chamber volume of 5 ml is installed For further information see User Manual chapter Maintenance 5 Prime all Q inlets and purge the System pumps See Section 8 3 Prime Q inlets and purge System pump A and System pump B on page 76 90 KTA avant Installation Guide 29 0351 82 AA Start the test 9 Performance tests 9 3 Q valve test Follow the instruction below to start the Q valve test Step Action 1 In the System Control module select System Performance Test and Report Result The System Performance Test and Report dialog opens In the System Performance Test and Report dialog e Select Q Valve Test e Click the Run Performance Method button Result The Start Protocol of the Q valve test opens
61. n below to purge the Quaternary valve System pump A and System pump B Note that both pump heads of each System pump have to be purged Step Action 1 In the Manual instructions dialog e Select Pumps and pressures Pump wash and select All from the BufferPro Q inlets drop down list Manual instructions System3 Selected column type Select Instructions an Parameters for Pump wash Pumps and pressures a System flow Sample flow Off be Gradient Inlet B Pump wash System wash Off 4 Quaternary start concentrations ButferPro Q inlets Quatemary gradient BufferPro pH N Column packing flow Sample inlet Flow path Monitors g of i Save result as _ Browse 2 Auto update of parameters during run e 2 Click l Result A simultaneous pump wash of all the Q inlets is started This will re move air from the Quaternary valve 2 Wait until the pump wash is completed KTA avant Installation Guide 29 0351 82 AA 79 8 Prime inlets and purge pumps 8 3 Prime Q inlets and purge System pump A and System pump B Step Action 3 Connect a 25 30 ml syringe to the left purge valve of the selected System pump Make sure that the syringe fits tightly into the purge connector 4 Open the purge valve by turning it counterclockwise about 3 quarters of a turn Draw 10 ml of liquid slowly into the syringe with a rate of about 1 ml per second 5 Close the purge valve by turning it clockwis
62. nantedhtccecsettidesti aman henacenaeninny 105 95 Test protocol Tor O valve LEST psiniscnnnisinanaaii n aa 107 9 6 mest protoc l for System test x ssissscscsessassssassescscescovevsessvsenctsdzscaccascsseasbecsosesiveevusteaaustbssncsesrvevtosiins 109 Reference information 10 1 System specifications 10 2 Component SpECIfEAUONS ennn N NAAR NN 114 10 3 Wetted materials 121 10 4 Chemical resistance guide seeessesssessccsscsssssssssssssseseccssssssssuussssessccesssssssussuscsssssesssssssssssssessssscesees 124 KTA avant Installation Guide 29 0351 82 AA 1 Introduction 1 Introduction About the Installation Guide The purpose of the Installation Guide is to give the necessary instructions to enable users and service personnel to unpack an AKTA avant system delivered from the factory install the instrument install the computer install the software verify functionality after installation Read the entire Installation Guide before starting to install the AKTA avant system In this chapter This chapter contains the following sections Section See page 1 1 Important user information 6 1 2 Regulatory information 8 AKTA avant Installation Guide 29 0351 82 AA 5 1 Introduction 1 1 Important user information 1 1 Important user information Read this before using the AKTA avant system All users must read the entire KTA avant Installation Guide and Getting Started with AKTA avant before in
63. nd purge pumps 8 2 Prime sample inlets and purge the Sample pump Step Action 3 Open the purge valve by turning it counter clockwise about 3 quarters of a turn Draw a small volume of liquid slowly into the syringe with a rate of about 1 ml per second 4 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 5 Connect the syringe to the purge valve on the right pump head of the Sample pump and repeat steps 2 3 Keep the sample flow running 74 AKTA avant Installation Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 2 Prime sample inlets and purge the Sample pump End the run Click the End icon in the System Control toolbar to end the run El AKTA avant Installation Guide 29 0351 82 AA 75 8 Prime inlets and purge pumps 8 3 Prime Q inlets and purge System pump A and System pump B 8 3 Prime Q inlets and purge System pump A and System pump B Overview The procedure consists of the following steps 1 Prepare the system before priming the Q inlets and purging the System pumps Prime all Q inlet tubing 2 3 Purge the Quaternary valve and the System pumps 4 End the run Prepare the system before priming the Q inlets and purging the System pumps Note Step Inlet A1 and B1 must be immersed in buffer or water When the System pumps are synchronized the Inlet valves are positioned to A1 and B1 for a short mo ment Action 76 Make su
64. nstallation Guide 29 0351 82 AA Parameter 10 Reference information 10 2 Component specifications Data Optional placement KTA avant 25 Before Sample inlet valve or after Injection valve KTA avant 150 After Injection valve Sensing principle Ultrasonic Outlet valve fractionation Parameter Data Number of outlets 10 expandable to 32 Fraction volumes KTA avant 25 0 01 to 100 000 ml KTA avant 150 1 to 100 000 ml Delay volume UV Outlet valve AKTA avant 25 142 ul AKTA avant 150 535 ul Mixer Parameter Data Mixing principle Chamber with magnetic stirrer Mixer volume KTA avant 25 0 6 1 4 or 5 ml KTA avant 150 1 4 5 or 15 ml KTA avant Installation Guide 29 0351 82 AA 117 10 Reference information 10 2 Component specifications Gradient formation Parameter Data Gradient flow rate range KTA avant 25 e Binary 0 25 to 25 ml min e Quaternary 0 5 to 25 ml min KTA avant 150 e Binary 1 to 150 ml min e Quaternary 2 to 40 ml min Gradient composition accuracy Binary 0 6 Quaternary 1 Pressure monitors Placement of sensors System pump Range O to 20 MPa 2900 psi Accuracy 0 02 MPa or 2 whichever is greater UV monitor Wavelength range 190 to 700 nm in steps of 1 nm up to 3 wavelengths Absorbance range 6 to 6 AU Linearity within 2
65. nter Recommended Note The exact search path depends on the location of the UNICORN installation folder e Ifthe option Print report was selected in the Evaluation Procedures dialog in the Start Protocol of the test the report is automatically printed on the system printer Note that a system printer must have been set up see UNICORN Administration and Technical Manual Print the report and check the status of the tests For each of the tests the report states The test passed or The test failed Possible causes of a failed test The table below describes possible causes of a failed test When possible sources of error have been checked and taken care of run the test once again Action Faulty air sensor For further information see User Manual chapter Trou bleshooting Incorrect preparation of Make sure that the tubing was correctly prepared see tubing Prepare the test on page 82 84 AKTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 2 Fraction collector test 9 2 Fraction collector test Method description The Fraction collector test checks the functionality of the Fraction collector The method run takes approximately 10 minutes For KTA avant 25 fractionation of 2 ml is performed in three sequential wells in each of two 96 deep well plates For AKTA avant 150 fraction ation of 20 ml is performed in three sequential tubes placed in each of two Cassettes for 50 ml tubes
66. ons E Pumps and pressures amp Start concentration Q1 0 0 100 0 System flow fi Ze Sample flow 100 0 5 Gradient Start concentration Q2 10 0 100 0 Pump wash f O S 0 0 5 Start concentration Q3 0 0 100 BufferPro pH a 0 0 5 __ Column packing flow Start concentration Q4 10 0 100 0 Flow path N T O Monitors F PE Save result as Browse iO Auto update of parameters during run Click AKTA avant Installation Guide 29 0351 82 AA 77 8 Prime inlets and purge pumps 8 3 Prime Q inlets and purge System pump A and System pump B Step Action 3 Connect a 25 30 ml syringe to one of the purge valves of either of the system pumps Make sure that the syringe fits tightly into the purge connector 4 Open the purge valve by turning it counterclockwise about 3 quarters of a turn Draw 10 ml of liquid into the syringe Check that the Q1 inlet is filled with liquid 5 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 6 Repeat steps 2 5 for Q2 Q3 and Q4 respectively by setting the respective Quaternary start concentration to 100 Tip The piece of inlet tubing that is immersed in distilled water should be the last piece of inlet tubing to be primed 78 AKTA avant Installation Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 3 Prime Q inlets and purge System pump A and System pump B Purge the Quaternary valve and the System pumps Follow the instructio
67. re that the piece of waste tubing connected to Injection valve port W1 of the Injection valve is placed in a waste vessel Open the System Control module and select Manual Execute Manual In structions Result The Manual instructions dialog opens In the Manual instructions dialog e Select Flow path Injection valve and e choose System pump waste from the Position drop down list Click E Result The injection valve switches to the waste position Note This is necessary in order to achieve a low backpressure during the purge procedure AKTA avant Installation Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 3 Prime Q inlets and purge System pump A and System pump B Step Action 4 Select Pumps and pressures System flow e For KTA avant 25 Set the Flow rate to 1 0 ml min e For KTA avant 150 Set the Flow rate to 10 0 ml min Click Result A system flow starts Prime the Q inlets Follow the instruction below to prime the Q inlets Step Action 1 Make sure that the pieces of inlet tubing marked A1 B1 and Q1 Q4 are im mersed in the correct buffers 2 In the Manual instructions dialog e Select Pumps and pressures Quaternary start concentrations e Set Start concentration Q1 to 100 Make sure that the other start concentrations are set to 0 Manual instructions System3 Selected column type Select Instructions Parameters for Quaternary start concentrati
68. re the system Step Action 1 72 Make sure that the piece of waste tubing connected to Injection valve port W1is placed in a waste vessel Open the System Control module and select Manual Execute Manual In structions Result The Manual instructions dialog opens AKTA avant Installation Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 2 Prime sample inlets and purge the Sample pump Step Action 3 In the Manual instructions dialog e Select Flow path Injection valve and select Sample pump waste from the Position drop down list Click Execute Result The Injection valve switches to waste position This is necessary to achieve a low backpressure during the purge procedure e Select Pumps and pressures Sample flow For AKTA avant 25 set the Flow rate to 1 0 ml min For AKTA avant 150 set the Flow rate to 10 0 ml min Click Execute _ Result A sample flow starts Purge the Sample pump Follow the instruction below to purge both the pump heads of the Sample pump Step Action 1 In the Manual instructions dialog e Select Flow path Sample inlet e Select Buffer from the Position drop down list click Execute J Result The Sample inlet valve switches to Buffer port 2 Connect a 25 30 ml syringe to the purge valve of the left pump head of the Sample pump Make sure that the syringe fits tightly into the purge connector AKTA avant Installation Guide 29 0351 82 AA 73 8 Prime inlets a
69. responding absorbance values in the upper left corner of the Chromatogram pane Enter the values in column 2 in the tables in Section 9 5 Test protocol for Q valve test on page 107 as follows Enter the absorbance value at 0 UV 100 Q2 in table Step response test result Q1 Q2 Step response test result Q3 Q4 Step response test result Q1 Q2 Q3 Q4 100 UV 100 Q1 Step response test result Q1 Q2 Step response test result Q3 Q4 Step response test result Q1 Q2 Q3 Q4 5 Q1 95 Q2 Step response test result Q1 Q2 95 Q1 5 Q2 Step response test result Q1 Q2 5 Q3 95 Q4 Step response test result Q3 Q4 95 Q3 5 Q4 Step response test result Q3 Q4 50 UV 25 Q1 25 Q2 25 Q3 25 Q4 Step response test result Q1 Q2 Q3 Q4 KTA avant Installation Guide 29 0351 82 AA 93 9 Performance tests 9 3 Qvalve test Ill Th ustration of chromatogram e illustration below shows a chromatogram from a Q valve test The gradient steps are marked in the illustration 480 470 460 450 440 UV 1_265_Chrom 1 Q Valve Test 001 50 80 90 100 110 120 130 140 150 168 170 0 UV 100 UV 5 Q1 UV 95 Q1 UV 5 Q3 UV 95 Q3 UV 50 UV 95 Q2 5 Q2 95 Q4 5 Q4 Note The illustration shows a chromatogram from a Q valve test on AKTA avant 25 A chromatogram from a Q valve test on
70. ride Hydrochloric acid max 7647 01 0 231 595 7 CIP 0 1 M midazole 2M 288 32 4 206 019 2 2 M needed for buffer preparation sopropanol 100 67 63 0 200 661 7 CIP fatty gels Methanol 100 74 93 1 200 659 6 RPC CIP Mercaptoethanol 20mM 37482 11 4 253 523 3 Reducing agent n Propanol 50 67 63 0 200 661 7 RPC Phosphoric acid 0 1M 7664 38 2 231 633 2 CIP Potassium 1M 7778 77 0 231 913 4 HIC phosphate Potassium chloride 4M 7447 40 7 231 211 8 4M for pH cell stor age Note Long term use 1 month Sodium dodecyl 1 151 21 3 205 788 1 Detergent sulfate SDS Sodium chloride 4M 7647 14 5 231 598 3 CIP Sodium hydroxide 2M 1310 73 2 215 185 5 CIP Sodium sulphate 1M 7757 82 6 231 820 9 HIC Trichloroacetic acid 1 76 03 9 200 927 2 RPC additive Trifluoroacetic acid 1 176 05 1 200 929 3 RPC additive pep tide separation Triton X 1 9002 93 1 Triton X 100 Detergent Tween 1 9005 64 5 500 018 3 Detergent Tween 20 AKTA avant Installation Guide 29 0351 82 AA 127 10 Reference information 10 4 Chemical resistance guide Chemical Concen tration CAS no EC no Urea 8M 57 13 6 200 315 5 Buffer additive de naturizing agent Water 100 7732 18 5 231 791 2 Note Long term use 1 month 1 PEEK tubing is biocompatible and chemically inert to most solvents used in purification of proteins It has in general very good pressure limits especially for
71. se EMC requirements EN standard is harmo nized with EU directive 2004 108 EC EN 301 489 1 EN Electromagnetic compatibility and EN standard is harmo Radio spectrum Matters ERM Short Range Devices SRD Radio equip ment in the frequency range 9 kHz to 25 MHz and inductive loop sys tems in the frequency range 9 kHz to 30 MHZ 301 489 3 Radio spectrum Matters ERM nized with EU directives ElectroMagnetic Compatibility EMC 1999 5 EC and standard for radio equipmentand 2004 108 EC services EN 300 330 2 Electromagnetic compatibility and EN standard is harmo nized with EU directive 1999 5 EC KTA avant Installation Guide 29 0351 82 AA 1 Introduction 1 2 Regulatory information FCC statement The AKTA avant instrument excluding transmitted radio frequency energy from the Mixer M9 and UV detector U9 D complies with FCC 47 CFR Part 15b Federal Communi cations Commission FCC title 47 of the Code of Federal Regulations CFR Part 15b Radio Frequency Devices Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated ina commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction
72. st starts Overview of the Start Protocol dialogs The table below describes the dialogs of the Start Protocol Dialog Description Notes Displays the Method Notes of the method The Method Notes contains a method description and instructions on how to run the method This dialog also allows the user to enter Start Notes Evaluation Procedures Allows the user to select to save the report to file recom mended and or to print the report Result Name and Loca tion Allows the user to change result name and result location During the run A Message dialog opens during the run Read the messages in the dialog and make sure that necessary preparations have been performed Click the Confirm and Continue button in the Message dialog to change system state from System Pause to Run and proceed with the test Alternatively click the Confirm button in the Message dialog and click the Continue button on the Instrument display KTA avant Installation Guide 29 0351 82 AA 83 9 Performance tests 9 1 Air sensor test Automatic evaluation The system automatically generates a report when the test is finished The report can be printed in two ways e Ifthe option Save the report to file was selected in the Evaluation Procedures dialog in the Start Protocol of the test the report is saved in location C Program Files GE Healthcare UNICORN Temp Open the report and print it using your default Windows pri
73. stalling operating or maintaining the instrument Do not operate the AKTA avant system in any other way than described in the user documentation Otherwise you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Intended use AKTA avant is a liquid chromatography system intended for process development The system can be used to screen for optimal choice of columns media and running param eters to purify selected proteins The AKTA avant system is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of the product See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood 6 AKTA avant Installation Guide 29 0351 82 AA 1 Introduction 1 1 Important user information Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood Notices NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes
74. t Inlet valve A switches to the selected port Connect a 25 30 ml syringe to the purge valve of the left pump head of System pump A Make sure that the syringe fits tightly into the purge con nector Open the purge valve by turning it counter clockwise about 3 quarters of a turn Draw a small volume of liquid slowly into the syringe with a rate of about 1 ml per second KTA avant Installation Guide 29 0351 82 AA 69 8 Prime inlets and purge pumps 8 1 Prime buffer inlets and purge System pump A and System pump B Step Action 5 Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 6 Connect the syringe to the purge valve on the right pump head of System pump A and repeat steps 5 6 Keep the system flow running End the run Click the End icon in the System Control toolbar to end the run Lad 70 AKTA avant Installation Guide 29 0351 82 AA 8 Prime inlets and purge pumps 8 2 Prime sample inlets and purge the Sample pump 8 2 Prime sample inlets and purge the Sample pump Overview The procedure consists of the following steps 1 2 3 4 Prime all sample inlet tubing to be used during the run Prepare the system before purging the Sample pump Purge the Sample pump End the run Prime sample inlet Follow the instructions below to fill all sample inlet tubing to be used in the run with appropriate buffer solution Step Action 1 Make sure
75. t starts Overview of the Start Protocol dialogs The table below describes the dialogs of the Start Protocol Dialog Description Notes Displays the Method Notes of the method The Method Notes contains a method description and instructions on how to run the method This dialog also allows the user to enter Start Notes tion Result Name and Loca Allows the user to change result name and result location During the run Check that an accumulator wash is performed at the beginning of the run During the accumulator wash the Dispenser head is positioned over the Waste funnel and liquid is squirted from the nozzle several times 88 AKTA avant Installation Guide 29 0351 82 AA Evaluate the result 9 Performance tests 9 2 Fraction collector test For AKTA avant 25 Check that correct volume 2 ml is collected in each of the wells 1A1 1A3 and 6A1 6A3 Also check that the fractionation marks in the chromatogram correspond to the filled wells For KTA avant 150 Check that correct volume 20 ml is collected in each of the tubes 1A1 1A3 and 6A1 6A3 Also check that the fractionation marks in the chromatogram correspond to the filled tubes Possible causes of a failed test The table below describes possible causes of a failed test When possible sources of error have been checked and taken care of run the test once again Wrong volumes collect ed in the wells or tubes and disturbances on
76. that all sample inlet tubing that is to be used during the method run is immersed in the correct buffers In the Manual instructions dialog e Select Flow path Sample inlet e Select the Position of the inlet to be filled from the drop down list Manual instructions System5 Instructions mal Parameters for Sample inlet Pumps and pressures Position Flow path Injection valve C Column position Inlet A Inlet B pH valve Sample inlet Outlet valve njection mark Monitors amp Fraction collection Save result as 2 Auto update parameter fields will be updated during method run Click Cea Result The Sample valve switches to the selected port AKTA avant Installation Guide 29 0351 82 AA 71 8 Prime inlets and purge pumps 8 2 Prime sample inlets and purge the Sample pump Step Action 3 Connect a 25 30 ml syringe to one the purge valves of the pump heads of the Sample pump Make sure that the syringe fits tightly into the purge connector Open the purge valve by turning it counter clockwise about 3 quarters of a turn Draw liquid slowly into the syringe until the liquid reaches the pump Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents Repeat steps 2 5 for each sample inlet that is to be used in the method run Prepare the system before purging the Sample pump Follow the instruction below to prepa
77. the requirements for receiving the delivery box and storing the instrument before installation WARNING Heavy object The AKTA avant instrument weighs about 116 kg Use proper lifting equipment or use four or more persons when moving the instrument All lifting and moving must be performed in accordance with local regulations When you receive the delivery e Record on the receiving documents if there is any apparent damage on the delivery box Inform your GE Healthcare representative of such damage e Move the delivery box to a protected location indoors Delivery box AKTA avant instruments are shipped in a delivery box with the following dimensions and weight Contents Dimensions mm Weight KTA avant instrument with ac w1000 x h900 x d800 155 kg cessories Storage requirements The delivery boxes should be stored at a protected place indoors The following storage requirements must be fulfilled for the unopened boxes Parameter Allowed range Ambient temperature storage 25 C to 60 C Relative humidity 20 to 95 non condensing 22 AKTA avant Installation Guide 29 0351 82 AA Equipment for transportation 3 Site preparation 3 2 Delivery and storage The following equipment is recommended for handling the delivery boxes Equipment Specifications Pallet mover Suitable for a lightweight pallet 80 x 100 cm Cart for transporting the instru ment to the
78. ubleshooting Wrong mixer chamber size or faulty mixer Replace the mixer See User Manual chapter Maintenance Faulty Quaternary valve See User Manual chapter Troubleshooting Faulty System pumps See User Manual chapter Troubleshooting Incorrect preparation of buffer and tubing Make sure that the system was correctly prepared see Prepare the test on page 90 AKTA avant Installation Guide 29 0351 82 AA 95 9 Performance tests 9 4 System test 9 4 System test About this section The System test checks the solvent delivery the functionality of the UV and conductivity monitoring systems and the valve functionality The method run takes approximately 30 minutes This section describes how to prepare run and evaluate a System test In this section This section contains the following subsections Section See page 9 4 1 Prepare the test 97 9 4 2 Run the test 98 9 4 3 Evaluate the test 100 9 4 4 Possible causes of a failed test 105 96 AKTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 4 System test 9 4 1 Prepare the test 9 4 1 Prepare the test Required material The following material is required Distilled water 1 acetone and 1 M NaCl in distilled water Capillary loop Ref 1 Syringe 25 30 ml For AKTA avant 25 Mixer 1 4 ml For AKTA avant 150 Mixer 5 ml Prepare the test Follow the instructions below to prepare the system before m
79. urers Min Max Min Max ml 3 10 5 11 5 50 56 3 NUNC 8 12 13 3 96 102 8 BD Biosciences VWR 15 16 17 114 120 15 BD Biosciences 50 28 30 110 116 50 BD Biosciences Deep well plates Requirements The deep well plates used in the Fraction collector of KTA avant must fulfill the require ments listed in the table below Property Specification No of wells 24 48 or 96 Shape of wells Square not cylindrical Well volume 10 5 or 2 ml Approved deep well plates The plates listed in the table below are tested and approved by GE Healthcare to be used with AKTA avant AKTA avant Installation Guide 29 0351 82 AA 33 3 Site preparation 3 7 Required materials Plate type Manufacturer Part no 96 deep well plate Eppendorf 951033405 0030 501 306 BD Biosciences 353966 Greiner Bio One 780270 Porvair Sciences 219009 Seahorse Bioscience 30009 Whatman 7701 5200 48 deep well plate Seahorse Bioscience 30004 Whatman 7701 5500 24 deep well plate Seahorse Bioscience 30024 Whatman 7701 5102 34 AKTA avant Installation Guide 29 0351 82 AA 4 Hardware installation 4 Hardware installation About this chapter This chapter describes the installation procedure of an AKTA avant system In this chapter This chapter contains the following sections Section See page 4 1 Unpack the instrument 36 4 2 Install the computer equipment
80. urves faulty Conductivity monitor Unproportional peaks See User Manual chapter Troubleshooting faulty Sample pump Faulty baseline Perform a manual peak integration of the Cond curve See Evaluation Manual 106 AKTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 5 Test protocol for Q valve test 9 5 Test protocol for Q valve test Step response test result Q1 Q2 1 2 3 4 5 Programmed Value read Baseline corrected Normalized Allowed interval Gane mAU value value 100 UV 100 Q1 95 UV 95 Q1 5 93 6 96 4 Q2 5 UV 5 Q1 95 Q2 3 6 6 4 0 UV 100 Q2 Step response test result Q3 Q4 1 2 3 4 5 Programmed Value read Baseline corrected Normalized Allowed interval Caine mAU value value 100 UV 100 Q1 95 UV 95 Q3 5 93 6 96 4 Q4 5 UV 5 Q3 95 Q4 3 6 6 4 0 UV 100 Q2 AKTA avant Installation Guide 29 0351 82 AA 107 9 Performance tests 9 5 Test protocol for Q valve test Step response test result gt Q1 Q2 Q3 Q4 1 2 3 4 5 Programmed Value read Baseline corrected Normalized Allowed interval Cane mAU value value 100 UV 100 Q1 50 UV 48 6 51 4 25 Q1 25 Q2 25 Q3 25 Q4 0 UV 100 Q2 108 KTA avant Installation Guide 29 0351 82 AA 9 Performance tests 9 6 Test protocol for System test 9 6
81. water based buffers However organic solvents penetrates weaknesses in the tubing walls easier than water based buffers Special care should therefore be taken with prolonged use of organic solvents close to pressure limits 2 Ifhydrochloric acid HCI is used as a cleaning agent when columns are connected to the system the HCI concentration should not exceed 0 1 M in the pressure sensors Remember that the AKTA avant system has pressure sensors in the Column valve V9 C For other parts of the system up to 1 M HCl is acceptable for short time use Long time use of 0 2 M HCI connected to the Quaternary valve as part of a BufferPro recipe is acceptable The solution becomes diluted further down in the system 128 AKTA avant Installation Guide 29 0351 82 AA For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com AKTA imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company AKTA UNICORN and Whatman are trademarks of GE Healthcare companies Eppendorf is a trademark of Eppendorf AG Hastelloy is a trademark of Hayne s Intl Inc Seahorse Bioscience is a trademark of Seahorse Bioscience Inc Microsoft and Windows are trademarks of Microsoft Corporation 2009 2012 General Electric Company All rights reserved First published Dec 2012 All goods and services

ÄKTA avant Installation Guide

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&Auml;KTA avant Installation Guide

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ÄKTA avant Installation Guide