JLA-8.1000 and JLA-9.1000 rotor assemblies
Contents
1. see Run Speeds Number of removable canisters 00 cece cee e ene teen een 4 Available bottle see Table 1 Tmin Nominal dimensions of largest 95 191 mm Nominal bottle nnn 1000 mL lt Nominal rotor capacity ce s Leo Otia dd obe ve RR TRIBNS 4 liters f M Weight of fully loaded rotor with lid 20 8 kg 45 9 Ib Minimum installation weight rotor body and windshield 10 8 kg 23 9 lb Weight of single empty 630 gams 1 4 Ib Q Maximum sample load for each canister at rated speed including bottle plug cap closure and sample 1500 grams 3 3 Ib 1 Axis of Rotation Approximate acceleration time to maximum speed rotor fully loaded iiis iur ina rrt and Pares Ween veter PER TERRE Eds 21 2 min Approximate deceleration time from maximum speed rotor fully loaded max brake 3 min Maximum allowable imbalance of opposing 30 grams Rotor body materialis enirir doter eer or Ro Ere rre e one aluminum Canister 1 carbon fiber epoxy composite Plug material ty ss ccacsarwadadawad eiaeiiai polycarbonate or polyetherimide PEI Cap closure material polyphenylsulfone PPSU2. PN J TB 073GC February 2014 Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A Instructions For Use J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies For Use in Beckman Coulter Avanti J Series Centrifuges COULTER J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies PN J TB 073GC February 2014 Copyright 1997 2014 Beckman Coulter Inc All rights reserved No part of this document may be reproduced or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise without prior written permission from Beckman Coulter Inc Avanti Beckman Coulter the stylized logo and HarvestLine are trademarks of Beckman Coulter Inc and are registered in the USPTO All other trademarks service marks products or services are trademarks or registered trademarks of their respective holders Find us on the World Wide Web at www beckmancoulter com Printed in the USA Safety Notice Read all product manuals and consult with Beckman Coulter trained personnel before attempting to use this equipment Do not attempt to perform any procedure before carefully reading all instructions Always follow product labeling and manufacturer s recommendations If in doubt as to how to proceed in any situation contact your Beckman Coulter Representative Alerts for Warning Caution Important and Note WARNING ind
3. 500 mL bottle w cap assy EM 69 x 160 mm polypropylene 355607 445 adapter 363683 1 8000 8000 500 mL bottle w cap assy um PN J TB 073GC 5 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Table 1 Bottles and Tubes Used in the JLA 8 1000 and JLA 9 1000 Rotors Continued Bottle or Tube Required Accessory Max Max Speed Speed Max No RCF RCF Max Fill Tubes kfactor kfactor Dimensions Part Volume Part per in JLA in JLA and Volume Description Number mL Description Number Adapter 9 1000 8 1000 62 x 120 mm polypropylene 356011 250 adapters 362750 in 1 9000 8000 250 mL wide mouth bottle 363683 w cap assy 62 x 120 mm polycarbonate 356013 250 adapters 362750 in 1 9000 8000 250 mL wide mouth bottle 363683 w cap assy 29 x 104 mm polycarbonate 357000 45 adapters 3569967 in 1 9000 8000 50mL bottle w 363683 liquid tight cap assy 29 x 104 mm polypropylene 357001 45 adapters 3569967 in 1 9000 8000 50 mL bottle w 363683 liquid tight cap assy 29 x 104 mm polypropylene 357003 40 adapters 3569964 in 1 9000 8000 50 mL bottle w screw cap 363683 _ 29 x 104 mm polypropylene 357005 36 5 adapters 3569967 in 1 9000 8000 50 mL thickwall tube 363683 natural 356264 29 red 357369 29 mm cap green 357360 29 mm cap yellow 35
4. m 10 EQ 1 k o 3600 where is the angular velocity of the rotor in radians per second c 0 105 RPM Tmax is the maximum radius and rmin is the minimum radius After substitution 0 533 x 10 Inna min Eua RPM2 17 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use In the JLA 8 1000 rotor the k factor is 2482 for full 1000 mL bottles at 8000 rpm In the JLA 9 1000 rotor the k factor is 2544 for full 1000 mL bottles at 9000 RPM At reduced speeds the k factors will be different Calculate k factors for these containers using EQ 2 Use the k factor in the following equation to estimate the run time t in hours required to pellet particles of known sedimentation coefficient s in Svedberg units S pak EQ3 S Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows u y EQ 4 adj ctual run speed for the JLA 8 1000 rotor 9000 for the JLA 9 1000 rotor Run times can also be estimated from data established in prior experiments using a different rotor if the k factor of the previous rotor is known For any two rotors a and b t k EQ5 Run Speeds The centrifugal force at a given radius in a rotor is a function of speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rotational speed is adjusted so that identical samples are subjected to th
5. A988 14 Cap closure replacement assembly made from USP Class VI material set of six B10317 AutoVent plug replacement O rings made from USP Class VI material set of six includes Six large plug O rings A98818 Six AutoVent plug O rings A98816 B10320 Labware Replacement Part Numbers for Bottle Assemblies B29896 Cap closure and plug assembly set of two includes Two cap closures Two AutoVent polypropylene plugs with O rings Two additional large plug O rings Two additional AutoVent plug O rings B29895 29 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Supply List Part Description Number Plug assembly AutoVent polypropylene set of two 393898 includes Two polypropylene AutoVent plugs with O rings Two additional large plug O rings Two additional AutoVent plug O rings Bottles 1000 mL polypropylene made from USP Class VI material set of two A98814 Cap closure replacement assembly made from USP Class VI material set of six B29894 AutoVent plug replacement O rings made from USP Class VI material set of six B10320 includes Six large plug O rings A98818 Six AutoVent plug O rings A98816 Supplies NOTE For MSDS information go to the Beckman Coulter website at www beckmancoulter com Part Description Number Rotor Cleaning Kit 339558 Solution 555 1 qt 339555 Spinkote lubricant 2 oz 306812 Spatula po
6. Decontamination 24 Sterilization and Disinfection 25 Returning a Rotor 26 Supply List 27 Replacement Rotor Parts and Labware 27 Supplies 30 vii Contents Beckman Coulter Inc JLA 8 1000 and JLA 9 1000 Rotor Warranty Related Documents viii Illustrations Illustrations 1 The JLA 8 1000 Fixed Angle 3 2 The JLA 9 1000 Fixed Angle Rotor 4 3 1 000 mL Bottle Assemblies Used in the JLA 8 1000 and JLA 9 1000 Rotor Assemblies 8 4 The 500 mL Adapter for the JLA 8 1000 and JLA 9 1000 Rotors 9 5 Installing the Rotor JLA 9 1000 shown 11 6 canister Sleeve Washer 21 7 canister Dome Area 22 8 Lubricating the Lid Stem 23 Tables Tables Bottles and Tubes Used in the JLA 8 1000 and JLA 9 1000 Rotors 5 2 Relative Centrifugal Fields for the JLA 8 1000 and JLA 9 1000 Rotors 19 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Specifications for the JLA 8 1000 Fixed Angle Rotor Assembly NOTE The JLA 8 1000 rotor can be used in Avanti JXN 26 J 26S XP series Avanti J 26 XP series and in discontinued Avanti J 20 series and Avanti J 20 XP series centrifuges only Maximum speed ise iU bed He rb ebbe Ei 8000 RPM Critical speed 200 to 400 RPM Maximum solution density ss esses 1 2 g mL Relative Centrifugal Field at maximum speed ALT 222 8 MM ERES EUN ER
7. HarvestLine System provides a convenient method of loading recovering and storing samples The liners are loaded with sample through a funnel or fermentor hose A valve in the liner neck is sealed and the liner necks are folded to fit inside the 1000 mL bottles The bottles are sealed with rotor plugs and cap closures and the sealed bottles are placed into the rotor canisters for centrifugation After centrifugation the liner valves are cut off and the supernatant is decanted either for storage or disposal The liners can then be heat sealed for pellet storage or disposal This rotor manual does not contain instructions for use of the HarvestLine System HarvestLine System instructions are contained in Litpak 369263 which contains document J TB 093 HarvestLine System Liner Kit 369264 Prerun Safety Checks PN J TB 073GC Read the Safety Notice section at the front of this manual before using the rotor 1 Make sure that the rotor and lid are clean and show no signs of corrosion or cracking 2 Inspect the canisters for damage see About Composite Canisters later in this manual 3 Before each use inspect each bottle cap closure and plug for cracks or damage Do not use damaged components J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use 4 Check the chemical compatibilities of all materials used Refer to Chemical Resistances publication IN 175 included in the Rotors and Tubes CD 5 Use
8. U S A or Canada or contact your local Beckman Coulter Representative for detailed information about ordering parts and supplies For your convenience a partial list is given below Replacement Rotor Parts and Labware NOTE Inthe kits and assemblies listed below cap closures plugs and adapters are shipped with the O rings installed and one spare of each O ring is provided Part Description Number Bottle assembly 1000 mL polycarbonate made from USP Class VI material set of two A98812 includes 1000 mL polycarbonate bottles set of two 366751 Cap closure and plug assemblies set of two A99028 Bottle assembly 1000 mL polypropylene made from USP Class VI material set of two A98813 includes 1000 mL polypropylene bottles set of two A98814 Cap closure and plug assemblies set of two A99028 Bottle assembly 1000 mL polypropylene made from USP Class VI material with B29896 polyphenylene sulfide cap closure set of two includes 1000 mL polypropylene bottles set of two A98814 Cap closure and plug assemblies set of two B29895 Bottle assembly 1000 mL polycarbonate set of two 363676 includes Two 1000 mL polycarbonate bottles 366751 Two cap closure and plug assemblies 363680 Bottle assembly 1000 mL polypropylene set of two 363678 includes Two 1000 mL polypropylene bottles 366752 Two cap closure and plug assemblies 363680 Labware Kit 392574 includes One
9. centrifugal forces that can efficiently pellet cells from large volumes of culture media cell particles from tissue homogenates or subcellular organelles The JLA 8 1000 and 9 1000 rotor assemblies consist of a rotor body with attached windshield a rotor lid and lid knob and six JLA 8 1000 or four JLA 9 1000 removable carbon fiber composite canisters see Figure 1 and Figure 2 The canisters are interchangeable allowing the use of multiple sets of canisters with a single rotor body Figure 1 The JLA 8 1000 Fixed Angle 1 Lid 4 Canister 2 Windshield 5 Bottle 3 Rotor Body 6 Cap Closure The rotor body windshield and lid are made of anodized aluminum The canisters are made of carbon fiber epoxy composite with a nickel plated aluminum sleeve Two pins in the rotor drive hole seat alongside the centrifuge drive hub teeth preventing the rotor from slipping during centrifugation The lid tie down knob assembly secures the rotor to the centrifuge drive hub J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Figure 2 The JLA 9 1000 Fixed Angle Rotor 1 Lid 4 Canister 2 Windshield 5 Bottle 3 Rotor Body 6 Cap Clousure The rotors with bottle assemblies 363676 363678 A98812 and A98813 have been tested with and without HarvestLine System liners 369256 to demonstrate containment of microbiological aerosols under normal conditions of the associated Beckman Coulter centrifuge when used an
10. 200 3000 1870 1350 830 2000 995 766 533 2000 829 600 370 1000 249 192 133 1000 207 150 92 500 62 48 33 500 52 38 23 a Entries in this table are calculated from the formula RCF 1 12r RPM 1000 and then rounded to three significant digits b Not rounded PN J TB 073GC Relative Centrifugal Fields for the JLA 8 1000 Rotor 4000 Speed RPM Relative Centrifugal Fields for the JLA 9 1000 Rotor 2000 3000 4000 5000 6000 7000 8000 Speed RPM 19 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Care and Maintenance Care and Maintenance Cap Closure and Plug Inspection and Maintenance Plug and cap closure performance depend on proper use and maintenance 1 check the O rings before each use to make sure that they are in good condition and are completely dry Replace the O rings if they are damaged torn or dirty O ring integrity is the key to successful function of cap closure and plug assemblies 2 Do not lubricate the cap closure O rings or plug O rings If they are lubricated they will cause the assembly to seal too tightly during centrifugation If crazing or cracks appear on or in a cap closure or plug replace the cap closure and plug immediately with a new cap closure and plug assembly Do not continue to use damaged or suspect cap closures plugs or other as
11. 7361 29 blue 357362 29 mm cap 29 x 104 mm polypropylene 357007 50 adapters 3569964 in 1 9000 8000 50 mL thickwall tube 363683 _ no cap 29 x 104 mm polycarbonate 363647 40 adapters 3569964 in 1 9000 8000 50 mL open top tube 363683 29 x 104 mm polycarbonate 363664 40 adapters 3569964 in 1 9000 8000 50 mL tube w snap on 363683 cap 6 PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Table 1 Bottles and Tubes Used in the JLA 8 1000 and JLA 9 1000 Rotors Continued Bottle or Tube Required Accessory Max Max Speed Speed Max No RCF RCF Max Fill Tubes kfactor k factor Dimensions Part Volume Part per in JLA in JLA and Volume Description Number mL Description Number Adapter 9 1000 8 1000 17 119 mm conical 355663 15 adapters 356960C in 5 6500 6500 15 mL polypropylene 363683 EN open top tube Bio Vials polypropylene 566353 4 adapters 342098 in 1 9000 8000 14 55 capped 362750Cin 363683 a Maximum speeds listed are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of manufacturing variances no guarantee of performance is expressed or implied b Do not fill above the maximum fill line marked on the bottle Minimum fill volume for bottles 363676 and A98812 is 500
12. U kie 15 970 xg Atro AGLI 12 300 x g At rage 194mm 8530 X g k factor at maximum speed full 1000 1 2482 Conditions requiring speed reductions see Run Speeds Number of removable 6 Available bottle see Table 1 Nominal dimensions largest 95 191 mm min Nominal battle 5 bee abo oda bi acea 1000 mL Nominal rotor capacity cc cece ehh hehe 6 liters a Weight of fully loaded rotor with 34 6 kg 76 lb lnak Minimum installation weight rotor body and windshield 16 8 kg 37 Ib Weight of single empty canister 630 gams 1 4 Ib Maximum sample load for each canister at rated speed n including bottle plug cap closure and sample 1500 grams 3 3 Ib Approximate acceleration time to maximum speed rotor fully loaded ore cem rere on ene wens 6 min 1 Axis of Rotation Approximate deceleration time from maximum speed rotor fully loaded max brake sess 3 min Maximum allowable imbalance of opposing 30 grams Rotor body alumi
13. back of this manual for more information 4 Each canister has a polyetherimide PEI sleeve washer 363601 beneath the aluminum sleeve see Figure 6 which acts as a cushion between the canister and the rotor body Before each use make sure that the sleeve washer is securely in place If the canister is exposed to high temperatures and or autoclaving the washer may loosen if this happens press it back into place with your finger If the washer becomes deformed so that it no longer stays on the canister replace it About Composite Canisters PN J TB 073GC The carbon fiber epoxy composite material used in the JLA 8 1000 and JLA 9 1000 canisters is extremely strong and ideally suited to the high stresses produced by centrifugal force The canisters will retain their strength throughout their 7 year life but will exhibit certain cosmetic changes with time The canisters also require different inspection and handling procedures than all metal rotor components After several thousand centrifuge run cycles surface cracks may appear in the canister dome area see Figure 7 and may increase in number with continued use Surface cracks are not uncommon in carbon fiber components and do not affect the structural integrity of the canister However cracks or other damage on the outside wall should be inspected by a Beckman Coulter representative Repeated autoclaving of the canisters may cause the following conditions all of which are cosm
14. d maintained as instructed For warranty information see the Warranty at the back of this manual Each canister is marked with an individual serial number and expiration date do not use a canister after the expiration date Preparation and Use Specific information about the JLA 8 1000 and JLA 9 1000 rotor assemblies is given here Information common to these and other rotors is contained in the manual Rotors and Tubes for J Series Centrifuges publication JR IM 10 which should be used together with this manual for complete rotor and accessory information NOTE Although rotor components and accessories made by other manufacturers may fit in the JLA 8 1000 and JLA 9 1000 rotors their safety in these rotors cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in these rotors may void the rotor warranty and should be prohibited by your laboratory safety officer Use only the components and accessories listed in this publication in these rotors Validation of microbiological containment was done at an independent third party testing facility CAMR Porton Down UK or USAMRIID Ft Detrick MD U S A Improper use or maintenance may affect seal integrity and thus containment 4 PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Bottles and Tubes The JLA 8 1000 and JLA 9 1000 rotor assemblies use the bottles and tubes listed in Table 1 Be sure
15. d Angle Rotor Assemblies Preparation and Use Installing the Rotor 1 Hold the rotor by the scalloped areas between the canister cavities and carefully lower the rotor straight down onto the centrifuge drive hub see Figure 5 Do not hold the rotor by the windshield during installation NOTE Do not drop the rotor onto the hub or the hub could be damaged If the rotor is left in the centrifuge between runs make sure the rotor is seated on the drive hub and the tie down knob is tight before each run Figure 5 Installing the Rotor JLA 9 1000 shown 1 Rotor Scallop four total on JLA 9 1000 six on JLA 8 1000 2 Windshield 2 Turn the rotor slightly around the centrifuge drive hub to ensure that the rotor is properly seated 3 After canisters are installed place the lid on the rotor and tighten the lid tie down knob to secure the rotor to the centrifuge drive hub Loading the Canisters NOTE canisters run in opposite positions in the rotor must balance to within 30 grams of each other Load opposing canisters with the same type of labware containing the same amounts of fluid of equal density Do not exceed the rated maximum sample load for any single canister which is 1500 grams 3 3 Ib including bottle sample plug and cap closure PN J TB 073GC 11 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Working with Physiological Fluids Handle body fluids with care because t
16. d by this warranty for seven 7 years from the date of purchase and will be replaced or repaired during such period according to the terms and conditions of this warranty 5 Acanister should not be used after the expiration date marked on the canister If the marked date is fewer than 7 years from the date of purchase the expiration date becomes the date of purchase plus 7 years Use of a canister after such expiration date voids Beckman Coulter s warranty obligations with respect to any rotor and or centrifuge in which such a canister is used Warranty 1 Beckman Coulter Inc JLA 8 1000 and JLA 9 1000 Rotor Warranty Disclaimer IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT Warranty 2 PN J TB 073GC www beckmancoulter com Osoon COULTER Related Documents Rotors and Tubes for J2 J6 and Avanti J Series Centrifuges JR IM 10 Rotors Tubes Bottles and Accessories Using Tubes Bottles and Accessories Using Fixed Angle Rotors Using Swinging Bucket Rotors Using Vertical Tube and Rack Type Rotors Care and Maintenance Chemical Resistances for Beckman Coulter Centrifugation Products Temperature Compensation Tab
17. e polycarbonate bottles bottle plugs or the polyethylene bottle rack 974627 or spatula 363646 Use cold sterilization methods as described below Low concentrations of hydrogen peroxide less than 6 and bleach less than 10 may be used on all rotor components bottles and accessories Ethanol 70 may be used on all rotor components except the polycarbonate bottles and plugs Hydrogen peroxide and bleach may cause discoloration and corrosion of the anodized rotor surfaces Use the minimum exposure time for each solution rinse thoroughly with distilled water and dry with a soft cloth per laboratory standards Ethanol is a flammability hazard Do not use it in or near operating centrifuges No guarantee of sterility or disinfection is expressed or implied through the use of these disinfection methods When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use 25 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Returning a Rotor Refer to publication IN 192 included with each box of tubes for tube sterilization and disinfection procedures Returning a Rotor 26 Before returning a rotor or accessory for any reason prior permission must be obtained from Beckman Coulter Inc A return authorization form may be obtained from your local Beckman Coulter sales office The return form should contain the following information rotor type and serial numb
18. e same RCF in two different rotors the samples are subjected to the same force The RCF at a number of rotor speeds is provided in Table 2 Do not select rotational speeds that exceed the limits in Table 1 When solutions more dense than 1 2 g mL are centrifuged in the JLA 8 1000 rotor use EQ 6 to calculate the reduced maximum allowable rotor speed Use EQ 7 to calculate the reduced maximum allowable rotor speed for the JLA 9 1000 rotor 1 2 g mL EQ6 density of tube contents reduced maximum speed 8000 RPM 12 g mL EQ7 of tube contents reduced maximum speed 9000 RPM density The maximum speed for 1000 mL polypropylene bottles 363678 is 7000 RPM in the JLA 8 1000 and 8000 RPM in the JLA 9 1000 PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Table 2 Relative Centrifugal Fields for the JLA 8 1000 and JLA 9 1000 Rotors JLA 8 1000 Rotor JLA 9 1000 Rotor Relative Centrifugal Field x g Relative Centrifugal Field x g Rotor Rotor Speed At Imax At ray At Fmin Speed At Imax At rav At Fmin RPM 222 8 mm 171 mm 119 mm RPM 185 mm 134 mm 82 mm 9000 16 800 12 200 7440 8000 15 9705 12 300 8530 8000 13 300 9610 5880 7000 12 200 9380 6530 7000 10 200 7350 4500 6000 8950 6900 4800 6000 7460 5400 3310 5000 6220 4790 3330 5000 5180 3750 2300 4000 3980 3060 2130 4000 3320 2400 1470 3000 2240 1720 1
19. emblies MAX FILL 1000mL 750mL 500mL 27 Cap Closure polyphenylsufone PPSU or polyphenylene sulfide PPS Cap Closure O ring AutoVent Plug O ring AutoVent Plug Plug O ring Bottle 1000 mL polycarbonate or polypropylene a Applies to bottle assemblies 363676 and 363678 only A six place rack 974627 for carrying the bottles is provided with labware kit 392574 Extra racks may be ordered Bottle Rack 974627 The 500 mL adapter 363683 see Figure 4 enables the use of 500 mL 250 mL 50 mL and 15 mL bottles and tubes and Bio Vials Labware smaller than 500 mL must be placed in another adapter or in more than one other adapter before being placed in the 500 mL adapter See Table 1 for a list of bottles tubes and adapters that can be used See the Supply List for part numbrs PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Figure 4 The 500 mL Adapter for the JLA 8 1000 and JLA 9 1000 Rotors 1 Adapter Cap 2 Adapter O rings 970883 b b 3 Adapter Body HarvestLine System Liners The HarvestLine System 369264 consisting of nonsterile polyetherurethane liners that are placed inside the rotor bottles is available for use with the JLA 8 1000 and JLA 9 1000 rotors The
20. er history of use approximate frequency of use reason for the return original purchase order number billing number and shipping number if possible name and email address of the person to be notified upon receipt of the rotor or accessory at the factory name and email address of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Sterilization and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the outside of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the return form when mailing the rotor and or accessories Customers located outside the United States should contact their local Beckman Coulter office PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Supply List Supply List See the Beckman Coulter High Performance High Speed High Capacity Rotors Tubes amp Accessories catalog BR 8102 available at www beckmancoulter com call Beckman Coulter Customer Service at 1 800 742 2345
21. essary and remove it promptly from rotor surfaces For radioactive decontamination of the canisters RadCon Surface Spray and Radiacwash can be used Do not soak the canisters in these solutions for long periods use the minimum immersion times specified by the solution manufacturers While Beckman Coulter has tested these methods and found that they do not damage components no guarantee of sterility or disinfection is expressed or implied Consult your laboratory safety officer regarding the proper decontamination methods to use If the rotor or other components are contaminated with toxic or pathogenic materials follow appropriate decontamination procedures as outlined by your laboratory safety officer Refer to Chemical Resistances IN 175 to evaluate your rotor s ability to withstand a given chemical solution Strongly alkaline solutions will damage the rotor body Sterilization and Disinfection 121 C PN J TB 073GC The rotor body canisters cap closures polypropylene bottles and polyetherimide PEI plugs can be autoclaved at 121 C for up to 20 minutes Remove canisters from the rotor body during autoclaving Place polypropylene bottles into the autoclave upside down to prevent bottle deformation The 500 mL adapter adapter cap and O rings can be autoclaved at 121 C for 15 minutes Repeated autoclaving will reduce the life of these components For this reason cold sterilization methods are recommended Do not autoclav
22. etic in nature during the 7 year warranty period anincrease in the number of surface cracks in the dome area increased visibility of existing surface cracks achangein canister color due to oxidation of the epoxy and formation of a white powdery residue on the outside wall of the canister this residue can be wiped off with a soft damp cloth 21 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Care and Maintenance Repeated autoclaving may also cause the sleeve washer 363601 to deform so that it no longer stays on the canister If this happens replace the washer Figure 7 canister Dome Area JLA 8 1000 8000 RPM JLA 9 1000 9000 RPM seen 1 Outside Wall 2 Dome Area Rotor Body Inspection and Maintenance 1 Periodically at least monthly inspect the rotor body for rough spots pitting or heavy discoloration If any of these signs are evident do not use the rotor Contact Beckman Coulter Field Service for information about the Field Rotor Inspection Program FRIP and the rotor repair center 2 Before using the rotor inspect the rotor drive pins to ensure that they are not damaged Damaged drive pins can prevent the rotor from seating properly on the centrifuge drive hub Avanti J series centrifuges only To inspect the drive pins turn the rotor upside down and look into the drive hole in the center of the rotor If the drive pins appear damaged contact Beckman C
23. fective in which case Beckman Coulter will pay all transportation charges A defective rotor will be replaced by Beckman Coulter at its then current list price less a credit based upon the age of the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Indianapolis Indiana facility or delivered to a Beckman Coulter Field Service representative The replacement price cost to Buyer for the respective rotor shall be calculated as follows years Replacement price Current rotor list price x 7 Conditions 1 Except as otherwise specifically provided herein this warranty covers the JLA 8 1000 and the JLA 9 1000 rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i bottles ii bottle contents iii cap closures or iv plugs 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the JLA 8 1000 or JLA 9 1000 rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor characteristics or is operated in a Beckman Coulter centrifuge that has been improperly disassembled repaired or modified 4 Each canister whether purchased with a complete rotor assembly or purchased separately is covere
24. groove threaded areas inside and out for cracking b Regularly inspect the plug in the areas behind the O ring for cracking c Make sure that the O rings are in place and in good condition and that for the plug both the O ring and the plug bottle interface are completely dry Make sure that the large and small plug O rings are in place For the cap closure make sure that the area around the O ring is completely dry f Donotlubricate the O rings Place a plug on the bottle a Then place a cap closure over the plug and tighten firmly by hand to where the scribe mark is as closely as possible aligned with the vent line on the bottle or beyond the line if possible You cannot over tighten the closure by hand Tools are available to assist in tightening and loosening cap closures See Bottle Grip and Wrench Kit 36670 below 1 Tighten the cap closure until this mark is as closely as possible aligned with the bottle vent line FILL un 4000mL 2 Vent Line Bo 4H 5 Place the filled sealed labware assembly into an empty canister using a twisting motion to ensure proper seating a Place the canister into a cavity in the installed rotor Bottles MUST be placed into canisters NEVER place a bottle directly into a rotor cavity 6 Repeat steps 1 through 5 for all of the bottles to be run 7 Make sure that a canister is installed in every rotor position run empty canisters without cap closu
25. hey can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Take appropriate safety precautions when handling toxic pathogenic or other hazardous materials When working with potentially hazardous materials always fill and open containers in an appropriate hood or biological safety cabinet In addition it is strongly recommended that all containers carrying physiological fluids be capped to prevent leakage Loading the 1000 mL Bottle and Labware Assemblies 1 Inspect the bottles that you will be using especially in the areas shown in the illustration Do not use bottles that are cracked or deformed 2 Filla bottle with sample Do not fill above the maximum fill line marked on the bottle See Table 1 for complete fill volume and run speed information 1 Do not fill above this line 2 Inspect here 3 Regularly inspect the cap closure you will be using 1 Cap Closure 5 Plug Bottle Interface CN LO P j 2 O ring 6 Inspect here OR A 3 Plug 7 AutoVent Plug O ring 0 oso IL a Applies to bottle assemblies 363676 and 363678 only PN J TB 073GC PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use a Inspect the O ring
26. icates a potentially hazardous situation which if not avoided could result in death or serious injury CAUTION indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury NOTE NOTE is used to call attention to notable information that should be followed during installation use or servicing of this equipment Safety Information for the J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies PN J TB 073GC These rotors were developed manufactured and tested for safety and reliability as part of a Beckman Coulter centrifuge rotor system Their safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter s approval Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe conta
27. inment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor s and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the centrifuge Safety Notice Safety Information for the J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories as required Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on Run Speeds Do not use sharp tools on these rotors that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use PN J TB 073GC Safety Notice Safety Information for the J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Additional information for the safe use of the J Lite JLA 8 1000 and JLA 1 000 rotor assemblies Please read before use Use all rotor components during each run as shown Six canisters must be run at all times in the JLA 8 1000 and four in the JLA 9 1000 whether loaded or empty Do not place cap closures on top of empty canisters NEVER load sa
28. les Gradient Materials Blood Component Separation References Glossary Available in hard copy or electronic pdf by request Rotors and Tubes CD 369668 Rotors and Tubes for Tabletop Preparative Ultracentrifuges Rotors and Tubes for J2 J6 Avanti J Series Centrifuges Rotors and Tubes for Preparative Ultracentrifuges Rotor Safety Bulletin Chemical Resistances for Beckman Coulter Centrifugation Products Included with shipment of instrument Additional References Chemical Resistances for Beckman Coulter Centrifugation Products IN 175 Beckman Coulter High Performance High Speed High Capacity Rotors Tubes amp Accessories catalog BR 8102 HarvestLine System Liner Kit 369264 J TB 093 Use and Care of Centrifuge Tubes and Bottles IN 192 Available in hard copy or electronic pdf by request also available at www beckmancoulter com 2014 Beckman Coulter Inc All Rights Reserved
29. lyethylene for removing pellet from 1000 mL bottles 363646 Polytetrafluorethylene PTFE spray 366772 Tri flow lubricant 2 oz 392645 NOTE Suggested Permanent Marking Pens Sanford Laundry Marking Pen item 31161 black Sanford Permanent Metallic Marker item 76115 gold Sanford Permanent Metallic Marker item 68820 silver 30 PN J TB 073GC PN J TB 073GC Beckman Coulter Inc JLA 8 1000 and JLA 9 1000 Rotor Warranty Subject to the conditions specified below and the warranty clause of the Beckman Coulter terms and conditions of sale in effect at the time of sale Beckman Coulter Inc agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of the JLA 8 1000 or JLA 9 1000 rotor to the original buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge all centrifuge parts required for repair Replacement Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the transportation charges collect unless the product is found to be de
30. mL minimum fill volume for bottles 363678 A98813 and B29896 is 1000 mL Polyethylene terephthalate PET Polypropylene oan Acetal Polypropylene package of 25 The specially designed Beckman Coulter 1000 mL bottles are sealed using attached plugs and patented cap closures see Figure 3 Silicone O rings on the plug and the cap closure create the seals the O rings must be free of defects dry properly seated and unlubricated to ensure sealing Bottles may be centrifuged at normal operating temperatures from 2 to 25 C bottle A98813 can be centrifuged up to 40 C but should be tested under simulated conditions first when working at temperatures below 2 C or above 25 C NOTE The useful life of plastic bottles varies slightly between lots but will largely depend on handling and usage We highly recommend that you pretest labware in the rotor using water samples to determine your optimal operating conditions The manufacturing process can cause small cosmetic flaws on polycarbonate and polypropylene bottles These flaws which may appear as irregular or scraped surface areas are cosmetic only They do not affect the function nor shorten the life of the bottle PN J TB 073GC Applies to bottle assemblies 363676 and 363678 only Preparation and Use J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Figure 3 1 000 mL Bottle Assemblies Used in the JLA 8 1000 and JLA 9 1000 Rotor Ass
31. mple directly into a canister Load sample into a bottle seal the bottle with a plug and a cap closure as described in this manual and place the sealed bottle WEIT inside a canister NEVER place a bottle directly into a rotor cavity Place filled sealed labware c ap Closure assemblies in canisters previously O ring installed in the rotor body AutoVent P Plug O ring Cap Closure Check cap closures plugs and their O rings ERN Labware before each run to ensure that they are in Assembly good condition The cap closures must be free of crazing or other defects The plug and cap closure O rings must be free of defects and must be dry and unlubricated to ensure seal integrity Before each use inspect each bottle cap closure and plug for cracks or p Canister Sleeve Washer damage Do not use damaged components Canister Place filled canisters in at least two opposing cavities place empty canisters in the other cavities Make sure that filled canisters are loaded symmetrically into the rotor and that opposing containers are filled to the same level with liquid of the same density Rotor Body Place the rotor lid on top of the rotor Saag deaths body and securely fasten the round Rotor Cavities tie down knob Opposing filled canisters plus labware and sample must weigh within 30 grams of each other Although rotor components and accessories made by othe
32. num Canister carbon fiber epoxy composite Plug polycarbonate or polyetherimide PEI Cap closure material polyphenylsulfone PPSU or polyphenylene sulfide PPS a The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration PN J TB 073GC 1 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Specifications for the JLA 9 1000 Fixed Angle Rotor Assembly Specifications for the JLA 9 1000 Fixed Angle Rotor Assembly NOTE Before the JLA 9 1000 rotor can be used in an Avanti J series centrifuge shipped before January 1998 a field upgrade of the centrifuge is required Contact Beckman Coulter Field Service for information Maximum speed uds tex e e hee Rt des atau P p e E oS 9000 RPM Critical speed 200 to 400 RPM Maximum solution density esses 1 2 g mL Relative Centrifugal Field at maximum speed nc etl Lo FRA d ti Ed 16 800 x g Atr 42405 eria cece agate tle boap UIN NR 12 200 x g TN p 7440 x g k factor at maximum speed full 1000 ml bottle 2544 Conditions requiring speed
33. only the bottles tubes and adapters listed in Table 1 b Rotor Preparation Before each run make sure the rotor is seated on the centrifuge drive hub with the rotor drive pins alongside the teeth on the top surface of the hub and that the lid knob is tight If you run less than a full load arrange the loaded canisters opposite each other in the rotor and place empty canisters in the other cavities a Fillopposing bottles to the same level with liquid of the same density b Placea canister loaded or empty in each rotor position during a run Make sure that the plug and cap closure O rings are in good condition are properly installed and that they are completely dry and unlubricated If the O rings or the sealing area are wet bottle leakage may result For low temperature runs from approximately 2 to 10 C precool the rotor canisters labware and sample in the centrifuge or in a refrigerator before use especially before short runs A suggested rotor precooling cycle is a minimum of 30 minutes at 2000 RPM at the required temperature After high temperature runs 40 C the cap closure may be difficult to remove a To prevent sticking spray a light coating of polytetrafluoroethylene PTFE spray 366772 on the top surface only of the plug b Allow the plug to dry completely before use Applies to bottle assemblies 363676 and 363678 only PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixe
34. or polyphenylene sulfide PPS a The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration b Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 2 9 where ris the radius in millimeters is the angular velocity in radians per second 2 x RPM 60 and g is the standard acceleration of gravity 9807 mm s2 After substitution 1 12r RPM 1000 2 2 PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Description Description PN J TB 073GC These rotors have been manufactured in a facility that maintains certifications to both ISO 9001 2008 and ISO 13485 2003 They are for use in the specified Beckman Coulter centrifuges The J Lite JLA 8 1000 fixed angle rotor assembly rated for 8000 RPM has a tube angle of 20 degrees from the axis of rotation and is used in Beckman Coulter Avanti JXN 26 J 26S XP series Avanti J 26 XP series and discontinued Avanti J 20 series and Avanti J 20 XP series centrifuges The J Lite JLA 9 1000 fixed angle rotor assembly rated for 9000 RPM also has a tube angle of 20 degrees from the axis of rotation and is used in all Beckman Coulter Avanti J series centrifuges These rotors develop
35. oulter Field Service 3 Do not use sharp tools on the aluminum rotor body as corrosion begins in scratches and may open fissures in the metal with repeated use If the rotor body is scratched contact Beckman Coulter Field Service about having it re anodized Do not use sharp objects or tools on the composite canisters as the composite material is easily damaged Call 1 800 742 2345 U S A or Canada outside the U S contact your local Beckman Coulter office or visit us on the web at www beckmancoulter com 22 PN J TB 073GC Cleaning PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Care and Maintenance To prevent corrosion of the aluminum rotor body store the rotor in a dry environment with the lid removed Do not store the rotor in the centrifuge To prevent abrasion of the metal rotor tie down components place the rotor lid upside down ona flat surface and put two or three drops of Tri Flow oil 392645 between the lid adapter and the lid stem see Figure 8 This area should be lubricated every few weeks or after each autoclave cycle Figure 8 Lubricating the Lid Stem 1 Lubrication Area Spinkote 2 Lid Stem 3 Lubricaton Points Tri Flow 4 Lid Adapter To provide lubrication between the rotor and the centrifuge drive hub apply a thin coat of Spinkote lubricant 306812 to the threads of the rotor tie down knob before each run If the rotor is left in
36. r manufacturers may fit in the JLA 8 1000 and JLA 9 1000 rotors their safety in these rotors cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in these rotors may void the rotor warranty and should be prohibited by your laboratory safety officer Use only the components and accessories listed in this publication in these rotors Cap closure O ring included only with bottle assemblies 363676 and 363678 PN J TB 073GC V vi Safety Notice Safety Information for the J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies PN J TB 073GC Contents s Safety Notice iii Alerts for Warning Caution Important and Note iii Safety Information for the J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies iii J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies 1 Specifications for the JLA 8 1000 Fixed Angle Rotor Assembly 1 Specifications for the JLA 9 1000 Fixed Angle Rotor Assembly 2 Description 3 Preparation and Use 4 Bottles and Tubes 5 HarvestLine System Liners 9 Prerun Safety Checks 9 Rotor Preparation 10 Installing the Rotor 11 Loading the Canisters 11 Temperature 16 Removal and Sample Recovery 16 Run Times 17 Run Speeds 18 Care and Maintenance 20 Cap Closure and Plug Inspection and Maintenance 20 Canister Inspection and Maintenance 20 About Composite Canisters 21 Rotor Body Inspection and Maintenance 22 Cleaning 23
37. res Opposing filled canisters plus labware and sample must weigh within 30 grams of each other Applies to bottle assemblies 363676 and 363678 only 13 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Bottle Grip and Wrench Kit 36670 It is very important that bottles be tightly sealed before a run to prevent leakage Some users may find it difficult to hand tighten the parts adequately Kit 366770 provided with labware kit 392574 contains a bottle grip and a wrench that are designed to simplify tightening and loosening cap closures to seal and unseal bottles Using the Bottle Grip The bottle grip seats on any flat surface and holds the bottle snugly in place leaving both hands free for loading and sealing It is not necessary to affix the grip to the surface pressing down as you turn the cap closure prevents the grip from moving 1 Inspect all parts as described in Loading the 1000 mL Bottle and Labware Assemblies above 2 Place an empty bottle in the grip with the bottle vent line toward you a Push down until the bottle is seated 1 Scribe Mark 2 Vent Line 3 Fill the bottle 4 place a plug on the bottle 5 Place a cap closure over the plug 14 PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use 6 Tighten the cap closure Without the Wrench Grasp the cap closure with both hands push down for le
38. sembly components Replace labware assemblies at the first sign of damage to any of the components Canister Inspection and Maintenance Regularly inspect each canister as described below 1 Before each use inspect the canisters 363686 for deep cracks If any cracking or other damage is visible on the outside wall do not use the canister Contact Beckman Coulter Field Service 2 Ifacrack or blemish appears after a canister is dropped from a height of several feet or after it strikes a sharp edge of a bench or table autoclave the canister and then return it to Beckman Coulter for inspection Include a written explanation of the reason for the return Applies to bottle assemblies 363676 and 363678 only t Call 1 800 742 2345 U S A or Canada outside the U S contact your local Beckman Coulter office or visit us on the web at www beckmancoulter com 20 PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Care and Maintenance 3 Each canister has an expiration date which is engraved on the aluminum sleeve around the top of the canister see Figure 6 Do not use the canister after this date Figure 6 canister Sleeve Washer 1 Aluminum Sleeve COULTER JLA 8 1000 8000 RPM JLA 9 1000 9000 RPM S N XXXX EXP DATE 6 19 2 Sleeve Washer 363601 MADE IN U S A New canisters can be used with the same rotor body See the Warranty at the
39. six place bottle rack 974627 One spatula 363646 One tool kit 366770 PN J TB 073GC 27 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Supply List 28 Description Part Number HarvestLine System liner kit includes 120 HarvestLine System liners nonsterile polyetherurethene USP 23 class VI 369256 One stainless steel filling rack 368740 One funnel 977472 Instructional CD 369367 369264 Bottle rack polyethylene holds six 1000 mL bottles 974627 Canisters set of two 363686 Canister sleeve washer set of six 363601 Lid assembly JLA 8 1000 363563 Lid assembly JLA 9 1000 363583 Tool Kit includes One bottle grip 366763 One wrench 367309 366770 500 mL Adapter assembly includes Adapter polyethylene teraphthalate PET One adapter cap polycarbonate One adapter O rings silicone set of two replacement part number 970883 363683 Labware Replacement Part Numbers for Bottle Assemblies 363676 and 363678 Cap closure and plug assembly set of two includes Two cap closures with O rings Two AutoVent polycarbonate plugs with O rings Two additional cap closure O rings Two additional large plug O rings Two additional AutoVent plug O rings 363680 Plug assembly AutoVent polycarbonate set of two includes Two polycarbonate AutoVent plugs with O rings Two addi
40. sures and plugs Use caution when opening the sealed assemblies Tools are available to assist in tightening and loosening cap closures See Bottle Grip and Wrench Kit 36670 above 16 PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use Removing Pellets from 1000 mL Bottles 1 Use the spatula provided to remove pellet from the bottle Do not use a metal tool to remove pellet as metal could scratch or damage the bottle and shorten the bottle s useful life Spatula for removing pellets 363646 Removing 500 mL Adapters 1 After centrifugation remove 500 mL adapters from the canisters by grasping each adapter cap top and pulling up until you can grasp the adapter body Then holding the adapter body remove the adapter completely and set it on a flat surface 2 Toremove the adapter cap hold the adapter firmly one hand and with the other hand twist and lift off the cap Placing your fingers in the indentations in the adapter may help you to grip the adapter tightly Some users may find the adapter caps difficult to remove To facilitate removal use the bottle grip tool described above Run Times PN J TB 073GC The k factor of the rotor is a measure of the rotor s pelleting efficiency Beckman Coulter has calculated the k factors for all of its rotors at maximum rated speed with full tubes or bottles using the following formula In fmax Tmin
41. the centrifuge between runs leave the centrifuge power off and the door open Refer to Chemical Resistances In 175 for chemical compatibilities of rotor and accessory materials Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues Manufactured by Thompson amp Formby Inc 10136 Magnolia Drive Olive Branch MS 38654 U S A 23 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Care and Maintenance 1 Wash the rotor canisters plugs cap closures and 500 mL adapters and adapter caps in a mild detergent such as Solution 555 339555 that will not damage the rotor and components Dilute the detergent with water 10 parts water to 1 part detergent The Rotor Cleaning Kit 339558 contains two special plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories 2 O rings on the 500 mL adapters can be left in place during cleaning unless spillage has occurred To remove them use fingers or a tool that will not damage the O rings Thoroughly wash and dry them before replacing them on the adapter Do not lubricate adapter O rings 3 Thoro
42. tional large plug O rings Two additional AutoVent plug O rings 366748 Plug assembly AutoVent polyetherimide PEI set of two includes Two polyetherimide PEI AutoVent plugs with O rings Two additional large plug O rings Two additional AutoVent plug O rings 366749 PN J TB 073GC PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Supply List Description Part Number Bottles 1000 mL polycarbonate set of two 366751 Bottles 1000 mL polypropylene set of two 366752 Cap closure replacement assembly set of six 366768 Cap closure replacement O rings set of six 366769 AutoVent plug replacement O rings set of six includes Six large plug O rings Six AutoVent plug O rings 366767 Labware Replacement Part Numbers for Bottle Assemblies A98812 and A98813 Cap closure and plug assembly set of two includes Two cap closures Two AutoVent polypropylene plugs with O rings Two additional large plug O rings Two additional AutoVent plug O rings A99028 Plug assembly AutoVent polypropylene set of two includes Two polypropylene AutoVent plugs with O rings Two additional large plug O rings Two additional AutoVent plug O rings 393898 Bottles 1000 mL polycarbonate made from USP Class VI material set of two 366751 Bottles 1000 mL polypropylene made from USP Class VI material set of two
43. to use only those items listed and to observe the maximum fill volumes and speed limits shown Refer to Chemical Resistances publication IN 175 for information on the chemical compatibilities of bottle and accessory materials Table 1 Bottles and Tubes Used in the JLA 8 1000 and JLA 9 1000 Rotors Bottle or Tube Required Accessory Max Max Speed Speed Max No RCF RCF Max Fill Tubes kfactor kfactor Dimensions Part Volume Part per in JLA in JLA and Volume Description Number mL Description Number Adapter 9 1000 8 1000 95 x 191 mm polycarbonate A98812 10005 none 9000 8000 1000 mL bottle with cap 16 800 15 970 closure and plug 2544 2482 made with USP Class VI material 95 x 191 mm polypropylene A98813 10005 none 9000 8000 1000 mL bottle with 16 800 15 970 polyphenylsulfone 2544 2482 cap closure and plug made with USP Class VI material 95 191 mm polypropylene B29896 10005 none 9000 8000 1000 mL bottle with 16 800 15 970 polyphenylene 2544 2482 sulfide cap closure and plug made with USP class VI material 95 x 191 mm polycarbonate 363676 10000 9000 8000 1000 mL bottle with cap 16 800 15 970 closure and plug 2544 2482 95 x 191 mm polypropylene 363678 10005 none 8000 7000 1000 mL bottle with cap 13 300 12 200 closure and plug 3220 3242 69 x 160 mm polycarbonate 355605 465 adapter 363683 1 9000 8000
44. ughly rinse the cleaned rotor and components with distilled water 4 Air dry the rotor lid and canisters upside down Do not use acetone to dry rotor components Do not wash the rotor components or accessories in a dishwasher Do not soak components in detergent solution for long periods of time such as overnight Do not use acetone methylethyl ketone MEK chloroform cyclohexane or organic solvents on the canisters at any time These substances will damage the epoxy resin surface material Decontamination e If the anodized aluminum rotor body becomes contaminated with radioactive material it should decontaminated using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solutions and found several that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash In the United States contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo t Inthe United States contact Biodex Medical Systems Shirley NY internationally contact the U S office to find the dealer nearest you 24 PN J TB 073GC J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Care and Maintenance IsoClean can cause fading of colored anodized surfaces Use it only when nec
45. verage and turn the cap closure to the right clockwise until the scribe mark on the cap closure is as closely as possible aligned with the bottle vent line or beyond the mark if possible Using the Wrench NOTE The wrench provides additional leverage for tightening and loosening the cap closure Hold the narrow end of the wrench in one hand and insert the two prongs in any position in the top of the cap closure Place your other palm on the flat textured area over the prongs Press down with your palm and tighten the cap closure until the scribe mark on the cap closure is as closely as possible aligned with the vent line on the bottle or beyond the mark if possible Loosen sealed bottles by pressing down and turning the wrench to the left counterclockwise 7 Remove the bottle from the bottle grip load it into a canister and then into the rotor b Loading the 500 mL Adapters Prepare the 500 mL adapter by making sure that the two O rings are in place as shown in Figure 4 Do not lubricate the O rings 2 Fill and seal the bottles or tubes to be used in the 500 mL adapters J 1f500 mL bottles are used place each bottle directly into a 500 mL adapter a For bottles of different sizes first load the bottles into the adapters listed in Table 1 and then place the filled bottle adapter assembly into the 500 mL adapters Do not load sample directly into the 500 mL adapter Place the adapter on the adapter push do
46. wn and twist The bottom edge of the adapter cap will not touch the top of the adapter but the assembly is sealed by the O rings and will seal completely during centrifugation PN J TB 073GC 15 J Lite JLA 8 1000 and JLA 9 1000 Fixed Angle Rotor Assemblies Preparation and Use 5 Hold the adapter by the adapter body not by the adapter and place the filled adapter into a canister No special orientation of the adapter in the canister is required Make sure that six canisters are loaded into the JLA 8 1000 rotor or four canisters in the JLA 9 1000 rotor prior to centrifugation Temperature Enter the run temperature according to the instructions in your centrifuge instruction manual NOTE To ensure that the rotor assembly reaches the required temperature during the run precool or prewarm the rotor canisters labware and sample to the required run temperature before starting the run Removal and Sample Recovery If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the ultracentrifuge and accessories Removing 1000 mL Bottles 1 Unscrew the rotor lid knob Remove the lid and set it aside 2 Grasp the metal handle on top of each cap closure and using a lifting and twisting motion lift the sealed bottle assemblies out of each canister If required place the sealed bottles under a safety hood before removing the cap clo
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