SHUTTLE BOX AVOIDANCE
Contents
1. Toggle P1 Bits Toggle P2 Bits AutoTest Clear Inputs Clear Outputs Exit ili ACCI CL TTI F RNY AVLOTNANCE MED ASSOC ES INC SHUTTLE BOX AVOIDANC CHAPTER 4 Beginning amp Running an Experiment Translating The MED PC IV mpc File Programs written in MedState Notation need to be translated using Trans IV before they can be executed in this application The MED PC IV Programmer s Manual explains how to accomplish this translation Be sure that a copy of the file being translated is present the directory C MED PC IV MPC Open Trans IV and select Translation Translate and Compile Select the program to use for the experiment and select Make Click OK to start the translator and it should automatically parse the MedState Notation and then open to a DOS screen to compile the Pascal code Depending on the speed of the computer each of these steps may not be seen If any problems are encountered during this process refer to the on screen help menu the MED PC IV User s Manual or contact MED Associates Inc for assistance Figure 4 1 Trans IV Control Panel for Translating and Compiling MedState Notation Code M Specify Files to Translate BAE Shuttle Box Avoidance With Four Inputs mpe Make Shuttle Box Avoidance With Pivot Floor mpc Shuttle Box Avoidance mpc Build Shuttle Box Passive Avoidance Test Level mpc Shuttle Box Passive Avoidance Test mpc Shuttle2. N BEAM 5 BREAK ITO I TOTO ITO ITO ITO ITO IO K Ke 5 8 25 81 START gt 52 S2 HR RightIR 1 Z Five gt 53 S3 HR RightIR 1 gt SX 0 01 gt 82 ko ko koe ko ko ke koe RH RH Ke N BEAM 6 BREAK 5 5 26 81 START gt S2 S2 HR RightIR 2 Z Six S3 S3 HR RightIR 2 gt SX 0 01 82 E E I ko ko ko koe ko ko AK AK K Ke N BEAM 7 BREAK 5 5 27 51 HSTART gt 52 S2 HR RightIR 3 Z Seven gt 53 S3 HR RightIR 3 gt SX 0 01 82 oko kc I dO K e N BEAM 8 BREAK 5 5 28 51 HSTART gt 52 S2 HR RightIR 4 Z Eight S3 S3 HR RightIR 4 gt SX 0 01 gt 82 DESCRIPTION OF STATE SET 32 State Set 32 counts the time for the session NCRCKCKCKCKCkCkCk kk A Kk KR Kk k kk kk kk ARR I N SESSION TEST TIMER 5 5 32 S1 START SHOW 5 Elapsed Time E 60 gt S2 S25 Wait for Start of First Trial Z Start CS S3 S3 Incr
3. Ke 5 5 9 51 HSTART gt S2 S2 0 01 IF A stimTag 1 Tone GNext Tone gt 53 Next IF A StimTag 2 Light Next Light gt S4 Next IF A StimTag 3 Both NoStim Both gt 85 GNoStim gt S1 S3 Tone Only Z Start CS IF L 1 Left Right Left ON LeftTone gt S6 Right ON RightTone gt S6 S4 Light Only Z Start CS IF L 1 Left Right Left ON LeftLight gt S6 GRight ON RightLight S6 S5 Both Tone amp Light 4z Start CS IF L 1 Left Right Left ON LeftTone LeftLight gt S6 GRight ON RightTone RightLight S6 S6 Wait for Signal to turn Stimuli OFF Z End CS OFF LeftTone LeftLight OFF RightTone RightLight S2 DESCRIPTION OF STATE SETS 10 amp 11 State Sets 10 and 11 record left and right movement within the chamber respectively RH RER IO dO K Ke LEFT MOVEMENT ACTIVITY E E 5 5 10 51 START gt S2 52 Wait for Start of First Trial Z Start CS 83 S3 Wait for first Beam Break Z Four gt 54 Z Three gt 55 Z Two gt S6 Z One gt S7 S4 Movement following a Beam 1 Break Z EndSession OT ADD B LeftMovement D I 5 gt 55 Z Two ADD B LeftMovement D I 5 gt S6 Z One ADD B LeftMovement D I 5 S7
4. Il Il il amp NN Control Variables with Assigned Aliases as Defined Var Alias Number of Trials to Run Default 50 Var Alias Stimuli 1 Tone 2 Lgt 3 Both Default Both Var Alias Avoid Interval sec Default 5 seconds Var Alias Escape Interval Default 25 seconds Default Yes Default Yes Default 60 minutes Var Alias CS UCS Overlap 1 Yes 0 Var Alias Punish ITI Cross 1 Yes O No Var Alias Session Time min AURWNES PEP ag gg Subscript Constants for Control Variables Trials 0 stimTag Time UCS Time overlapTag ITIShockTag SessionTime Il List Data Variables Here B Summary Data Array Subscript Constants for Summary Data Array TrialCount 0 Total Trials Run TotalAvoids 1 AvoidLatency 2 2 Divided by Total Avoids for Mean Latency TotalEscapes 3 EscapeLatency 4 Divided by Total Escapes for Mean Latency LeftMovement 5 RightMovement 6 TotalCrossings 7 TotalITIShocks 8 B 9 Not Used NV D Trial by Trial Data Array X D I Trial Number X D I 1 Avoid Tag N D I 2 Avoid Latency N 1 3 Escape Tag N 1 4 Escape Latency D I 5 Left Movement Activity X D I 6 Right Movement Activity N 1 7 Crossings D I 8 ITI Shocks 1 9 RESERVED List Working Variables Here Elapsed Time in Seconds I Subscri
5. SHUTTLE BOX AVOIDANCE SHUTTLE BOX AVOIDANCE LEVEL SHUTTLE BOX AVOIDANCE WITH FOUR INPUTS SHUTTLE BOX AVOIDANCE WITH FOUR INPUTS LEVEL SHUTTLE BOX AVOIDANCE WITH PIVOT FLOOR SHUTTLE BOX PASSIVE AVOIDANCE TEST SHUTTLE BOX PASSIVE AVOIDANCE TEST LEVEL SHUTTLE BOX PASSIVE AVOIDANCE TRAINING SHUTTLE BOX PASSIVE AVOIDANCE TRAINING LEVEL 11 MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE The Enter Experiment Data screen will appear next The purpose of this screen is to allow annotations to be added to the data file that is produced by MED PC IV These annotations will help identify the Subject Experiment and Experiment Group upon which data was collected Comments can be added here as well and the data file can be given a customized file name Enter the information desired and click Next the information on this panel is optional and can be skipped if so desired Figure 4 5 Enter Experiment Data Screen y Subject 1 Subject ee 8 Shuttle Box Level 1 Experiment Group Group 1 Comments 5 Box Level Experiment E Optional Custom Filename 5 Subiectt_Groupil The next screen to appear is the Review Choices screen This is a method of confirming that the information entered is correct If it is not correct select Previous and edit the data If it is correct select Next Figure 4 6 Review Choices Screen Review the data below Box 1 Procedure SHUTTLE BOX AVOIDANCE LEVEL Subject Subject 1 Ex
6. Z Escape Z End CS 513 813 Shock OFF Delay to Force Complete Crossing 1 5 OFF ShockOperate RightGrid S1 DESCRIPTION OF STATE SET 4 State Set 4 establishes the operation of the ITI punishment procedure If Crossings are being punished and once the ITI has initiated S2 the aversive stimulus sequence begins S3 The maximum duration of aversive stimulus is defined in S5 If ITI crossing are not punished then the sequence begins at S10 PUNISH ITI CROSSINGS 5 5 4 0 5 Shocks if Activated S1 HSTART gt S2 S2 Wait for Start of ITI Z Avoid Z Escape Z Start ITI IF A ITIShockTag 1 PunishCrossing NoShock GPunish gt S3 NoShock gt S10 S3 Start of Sequence with Shock Wait for Crossing dz EndSession gt S1 2 EnterLeft Z EnterRight ADD D I 7 B TotalCrossings ADD D I 8 B TotalITIShocks IF L 1 Left Right Left ON LeftGrid gt S4 Right ON RightGrid gt S4 Z Start CS S2 End of ITI 54 0 01 ON ShockOperate gt S5 2 EndSession OFF LeftGrid RightGrid gt S1 Z Start CS OFF LeftGrid RightGrid S2 S5 0 5 OFF ShockOperate LeftGrid RightGrid S6 2 EndSession OFF ShockOperate LeftGrid RightGrid gt S1 Z Start CS OFF ShockOperate L
7. Display Data from Box Additional Boxes to Update from 1 1 Select All Deselect Al 0 000 x The value of any simple variable may be viewed from this screen Click an array on the table and each element in that array can be viewed To change a value simply highlight and replace the value in the lower right hand box or use the up down arrows to increment by 1 Click the Issue button for the change to take effect Click Named Variables to produce the display in Figure 4 13 Change variables here as needed 15 MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE Figure 4 12 Displaying Array A Displaying Array A from Box 1 A 2 A 3 Alt S Named Vars 3 000 5 000 25 000 1000 Vars Refresh Issue Close a Help A 8 60 000 Display Data from Box Additional Boxes to Update 1 Select All Deselect All 01 from Box 1 50 000 Figure 4 13 Displaying Named Variables Displaying Named Variables from Box 1 Number of Trials to Run Stimuli 1 2 Lgt 3 Both Avoid Interval sec Escape Interval Befresh CS UCS Overlap 1 Yes 0 Punish ITI Cross 1 Yes 0 Session Time min Named Vars Issue Close Qui Help Select All Deselect All 16 CHAPTER 5 Saving Data amp Understanding the Raw Data File Data is automatically saved usi
8. Five ADD B RightMovement 1 Z Six ADD B RightMovement D 1 Z Seven ADD B RightMovement D 1 LeftMovement S1 54 55 57 51 54 55 56 51 55 56 57 51 54 56 57 51 54 55 57 51 54 55 56 DESCRIPTION OF STATE SETS 21 28 State Sets 21 28 record the IR inputs for each Beam exactly like Toggle Mode records inputs ko ko ko IK K Ke N BEAM 1 BREAK ke HR S 8 21 S1 START gt 52 S2 HR LeftIR 4 Z One gt S3 S3 HR LeftIR 4 SX 0 01 82 kc BEAM 2 BREAK RH IORI TOR ITO ITO ITO ITO RER HR S 6 22 S1 START gt 52 S2 HR LeftIR 3 Z Two S3 S3 HR LeftIR 3 SX 0 01 82 ko ko N BEAM 3 BREAK I IO oko RH ke 5 5 23 51 START gt S2 52 R LeftIR 2 Z Three gt 53 53 R LeftIR 2 gt SX DDL gt 62 E E RER N BEAM 4 BREAK oko kk IO dO Ke 5 8 24 51 START S2 S2 HR LeftIR 1 Z Four S3 S3 R LeftIR 1 gt SX 0 01 gt 82
9. does not avoid the presentation of the aversive stimulus then the CS will terminate after 5 seconds and aversive stimulus will be administered However if the subject avoids the aversive stimulus by moving to the other side of the chamber within 2 5 sec then the CS will terminate at 2 5 seconds and subject will not experience the aversive stimulus If the CS UCS Overlap 1 see below And the subject does not avoid the presentation of the aversive stimulus then the UCS aversive stimulus will be administered 5 seconds following the onset of the CS tone light The CS will remain on until either the subject escapes or the Escape Interval see below has expired Escape Interval sec This is the maximum duration of the UCS presentation The default value is set to 25 seconds but the UCS will terminate after the subject has moved to the other side of the chamber CS UCS Overlap 1 Yes 0 Enter 0 for this variable to terminate the CS light tone stimulus at the onset of the UCS aversive stimulus If this variable is set to 1 then the CS and UCS will terminate in unison Punish ITI Cross 1 Yes 0 No Enter 1 for this variable to enable the re administration of aversive stimulus For instance if the subject avoids or escapes the aversive stimulus and then returns to the side of chamber in which the aversive stimulus had originally occurred the program will re administer aversive stimulus Session Time min Defin
10. such as this one to make data collection easy These pre programmed applications can also be modified to meet the evolving demands of a research protocol This manual begins by providing step by step instructions on how to use this pre programmed application Then the manual provides some examples of editing and modifying the programming code and finally it defines the elements in the raw data file produced by this application In addition to this manual refer to the MED PC IV User s Manual for the installation of the MED Associates interface drivers the MED PC IV Software and the Delphi Compiler Also refer to the User s Manual for instructions on hardware configuration The Hardware Configuration software utility accompanies MED PC IV and is used to assign the inputs and outputs in the interface cabinet for each task controlled by the program Data file structure file saving format and other related options are also determined by the Hardware Configuration software utility Refer to the MED PC IV Programmer s Manual if you are unfamiliar with the process of translating and compiling an application Any lines in the mpc file that begin with a backslash V are comments that help identify key elements of the code and explain the function of each step within the program The staff at Med Associates Inc is available to answer any questions that may arise Please e mail us at support med associates com with a detailed description of the pro
11. Box Passive Avoidance Training Level mpc Shuttle Box Passive Avoidance Training mpc No Trans Exclude v X Cancel MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE Using the MED PC IV Load Wizard Open MED PC IV and the MED PC Experiment Loading Wizard s Welcome screen shown below will appear To proceed with the wizard click Next and the Box Selection screen shown in Figure 4 3 will appear Figure 4 2 The MED PC IV Loading Wizard Welcome Screen To avoid this load wizard deselect the checkbox labeled Run this experiment automatically when starting MED PC Close this screen by clicking the Close button Closing this screen immediately reveals the MED PC Run Time Screen shown in Figure 4 10 10 MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE The Box Selection screen is the screen in which the researcher chooses which boxes will be used in the experiment Select the boxes that will run the experiment by clicking in the radio button next to the box number The figure shows that the Hardware Configuration included only 1 box which was selected Click Next to proceed Figure 4 3 The Box Selection Screen The next screen is the Select a Procedure screen This is where the application to be run is selected The screen displays a list of all the currently compiled procedures Simply select the desired procedure for example Shuttle Box Avoidance Level and then click Next Figure 4 4 Select a Procedure
12. C IV mpc File sise 9 Using the MED PC IV Load Wizard 10 Viewing Changing Variable Values pp 15 Chapter B sm 17 Saving Data amp Understanding the Raw Data 17 Chapter ODORE CODE 19 Data Analysis Using MED PC to Excel Nt 19 Using a Pre Formatted Table Profile MTP file 19 Editing ciet pan TEE 22 ECEEREREECEREREEEEROREEEEPIREE EO ies 23 Modifying the Existing Program 23 Introduction Shuttle Box Avoidance Level mpc mne 23 DESCRIPTION OF STATE SET 1 6 then E prata reu eon Feu EE PER REA 23 DESCRIPTION OF STATE SET 2 2 2 trainees i o ERR resume GE S GG X 26 DESCRIPTION OF STATE SET 3 27 DESCRIPTION OF STATE SET 4530080 28 DESCRIPTION OF STATE SETS 5 8 622 cicius exer a x asap Ra Ra VAR PAR Ra AD 29 DESCRIPTION STATE SETS 7 amp Birisini ih Ra RR meets 29 DESCRIPTION OF STATE SET ni KR RERO ERR ERA AERA 30 DESCRIPTION OF STATE SETS 10 amp 11 30 DESCRIPTION OF STATE SETS 21 28 A 32 DESCRIPTION OF STATE SET 32 eee erret eth rh nerunt 33 CHAPTER 1 Introduction The latest version of MED PC IV gives researchers the ability to use pre programmed applications
13. ED PC IV run time environment without sending a start signal by selecting I am finished with the wizard This option results in the screen shown in Figure 4 10 Figure 4 10 MED PC IV Run Time Screen MED PC IV x File Configure View Macros Help ax ale amp 9 Subject Experi Group Load Program CS 8 12 2010 1 48 58 ea MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE Viewing Changing Variable Values Before a start command has been issued any variable may be changed on the MED PC IV run time screen Simply highlight the value to change and then type in the new value Once a session is in progress change variables by selecting Configure Change Variables on the main menu or click the 4th tool bar item AX In the lower left hand corner of the Displaying Variables window find the Display Data from Box display and choose the chamber s to modify By clicking additional boxes in the Additional Boxes to Update section changes made to a single box are automatically loaded on all of the boxes selected Figure 4 11 Displaying Variables Screen A Array B Array D Array m Named Vars H J Vars 0 000 0 000 0 000 0 000 0 000 M N 0 000 0 000 0 000 0 000 0 000 Refresh Q R T 0 000 0 000 0 000 S Array 0 000 5 Issue U y W K Y x ch 0 000 0 000 0 000 0 000 0 000 ose 2 0 000 A Help
14. Manual for details regarding the processing of commands in MED PC The MED PC IV Programmer s Manual and online tutorial provides necessary information on Med State Notation and these resources will be very helpful when making adjustments to Shuttle Box Avoidance Level mpc or creating a custom mpc program Shuttle Box Avoidance Level mpc WE DO LR LE TT 4 gt gt gt gt gt gt gt gt Copyright C 2010 MED Associates All Rights Reserved Shuttle Box Avoidance Level For systems with 8 I R Photobeam sensors separate Left and Right Light and Tone and either with or without Auto Guillotine Door The default values used for this Procedure run a fixed number of 50 Trials Each Trial presents both stimuli for 5 seconds followed by a maximum 25 second shock escape interval while the stimuli remain on The mean ITI interval is 15 seconds The total session time is set at 60 minutes however given the above parameters the test will always end in less than an hour Program runs only in Level Mode The 1 Beam is the Beam Closest to the Door or Hurdle on each Side Inputs are Assigned from Left to Right as viewed from front of Box Inputs LeftIR_4 LeftIR 3 LeftIR 2 LeftIR 1 RightIR 1 RightIR 2 RightIR 3 RightIR 4 Hog og 1 I U1 amp Outputs LeftLight RightLight LeftTone RightTone Door ShockOperate LeftGrid RightGrid Il
15. S5 Movement following a Beam 2 Break Z EndSession mulie Z Four ADD B LeftMovement D I 5 gt S4 Z Two ADD B LeftMovement D I 5 gt S6 Z One ADD B LeftMovement D I 5 gt S7 S5 Movement following a Beam 3 Break z EndSession Z Four ADD 1 Z Three ADD 71 Z One ADD B I S7 Movement following a Beam 4 Break z EndSession Z Four ADD 1 Z Three ADD 71 LeftMovement LeftMovement LeftMovement LeftMovement LeftMovement Z Two ADD B I TOR ITO ITO AO K K N RIGHT MOVEMENT ACTIVITY 5 5 11 S1 HSTART gt S2 S2 Wait for Start of First Trial Z Start CS S3 S3 Wait for first Beam Break Hz Five gt S4 2 51 gt 85 Z Seven gt S6 Z Eight gt S7 54 Movement following a Beam 1 Break Z EndSession Z Six ADD B RightMovement D 1 Z Seven ADD B RightMovement 1 2 Eight ADD B RightMovement D 1 S5 Movement following a Beam Z EndSession Z Five ADD B RightMovement D 1 Z Seven ADD B RightMovement 1 Z Eight ADD B RightMovement D 1 S6 Movement following a Beam Z EndSession Z Five ADD B RightMovement 1 Z Six ADD B RightMovement D 1 Z Eight ADD B RightMovement D 1 S7 Movement following a Beam Z EndSession Z
16. S6 4 amp z EnterRight ADD D I 1 B TotalAvoids ADD D I 7 B TotalCrossings Z Avoid Z End CS gt S1 S6 Activate Shock Left Side 0 01 ON ShockOperate S7 Z EnterRight ADD D I 1 B TotalAvoids ADD D I 7 B TotalCrossings 27 Z End CS gt S1 Process Avoid from S5 if it occurs same interrupt as CS timeout S7 UCS Period Left Chamber A UCS_Time T OFF ShockOperate LeftGrid Z Start ITI Z End CS S1 4Z EnterRight ADD D I 3 B TotalEscapes ADD 1 7 B TotalCrossings Z Escape Z End CS S8 S8 Shock OFF Delay to Force a Complete Crossing 1 5 OFF ShockOperate LeftGrid S1 510 S13 are identical in function to S5 S8 Above Nese tees eee seer euet ELE E EU S10 Trial Sequence Starting from Right Chamber CS Period A CS_Time T ON RightGrid IF A OverLapTag gt 1 OverLap NoOverLap GOverLap gt S11 GNoOverLap Z End CS S11 SZ EnterLeft ADD D I 1 B TotalAvoids ADD 1 7 B TotalCrossings Z Avoid Z End CS gt S1 S11 Activate Shock Right Side 0 01 ON ShockOperate S12 Z EnterLeft ADD D I 1 B TotalAvoids ADD 1 7 B TotalCrossings Z Avoid Z End CS gt S1 512 UCS Period Right Chamber A UCS Time T OFF ShockOperate RightGrid Z Start ITI Z End CS S1 Z EnterLeft ADD D I 3 B TotalEscapes ADD D I 7 B TotalCrossings
17. SHUTTLE BOX AVOIDANCE MED STATE NOTATION PROCEDURES SOF 700RA 7 User s Manual DOC 024 Rev 2 7 Copyright 2010 Rights Reserved MED Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE TABLE OF CONTENTS Chapter 1 sm 1 Introduction seni nn 1 Overview of the Procedure ss sain euenit aiani og I DEL ORE APA ada 2 Refererces e Edda 2 Active Avoidance Shuttle Box Avoidance Level mMpo 0 1 3 Independent Variables 40 ansa nnn 3 Dependent 4 26 ee Ee E 4 Passive Avoidance Training amp Testing Shuttle Box Passive Avoidance Training Level mpc amp Shuttle Box Passive Avoidance Test Level mpc 4 Independent Variables erc tok oett ces a 4 Dependent VariableSs Nb 5 Chapter 2 xaxa eee anne ce en aie een aie en ne En ne EIU OO OD UUOUR AU LOUER ERCOR 6 HardWares se en a E lassen EN ERE 6 Chapter Dm 7 iori I T 7 Installation se 7 Backing Up 7 Hardware TeSt em i on 8 Chapter dr 9 Beginning amp Running an Experiment ne 9 Translating The MED P
18. blem or your desired goals so that we may provide you with concise and detailed information The Shuttle Box Avoidance procedures are designed to be as easy to use as possible Med Associates understands that researchers do not have the time to devote to programming and hardware design and for that reason we have undertaken that burden for you We sincerely hope that you are satisfied with the products and services we provide and look forward to meeting your future experimental needs as your research program evolves Thank you for purchasing Med Associates Instrumentation and Software Overview of the Procedure The shuttle box task is a test that has been utilized to study conditioning in non human animals for sixty years Miller 1948 Mowrer amp Lamoreaux 1942 Sidman 1960 Shuttle box tests of avoidance and escape from aversive stimulus are used in contemporary research e g Amaropanth LeDoux amp Nader 2000 Caldarone et al 2003 because these behavioral assays are efficient and standardized methods for testing learning and memory processes Two types of procedures are generally used these being active and passive avoidance procedures Active avoidance is a negative reinforcement procedure the alleviation of aversive stimulus reinforces avoidance responding A stimulus signals that an aversive stimulus is imminent and the subject learns to avoid the aversive stimulus by moving to the other side of the two compartment chamb
19. coder Card Figure 2 1 Shuttle Box Hardware For detailed information regarding the wiring and connection of the Shuttle Avoidance Test Package refer to the Shuttle Box for Active amp Passive Avoidance Installation Guide CHAPTER 3 Software Installation Please refer to the MED PC IV User s Manual for a complete guide to installing the MED PC IV software The Shuttle Box Avoidance package includes a CD containing several MED PC testing protocols these include Shuttle Box Avoidance Level mpc Shuttle Box Passive Avoidance Training Level mpc Shuttle Box Passive Avoidance Test Level mpc Shuttle Box Avoidance With Four Inputs Level mpc Shuttle Box Avoidance With Pivot Floor mpc Avoid mtp MPC2XL transfer template Shuttle Box Avoidance mpc Shuttle Box Passive Avoidance Training mpc Shuttle Box Passive Avoidance Test mpc Shuttle Box Avoidance With Four Inputs mpc To install these testing protocols insert the CD into the CD ROM drive and click Install the Shuttle Box Software These files will be copied to the C MED PC IV MPC folder These protocols will not be automatically copied during installation If they are to be used they must be manually copied to the C MED PC IV MPC folder Backing Up the Software Med Associates strongly encourages creating backup copies of the Shuttle Box programs in case of disk failure Having copies of the original programs may be useful in the future should mo
20. difications be made to the existing programs MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE Hardware Test Open the MED Test software application Select SmartCtrl DIG 716B DIG 703B from menu bar On the SmartCtrl DIG 716B display verify that the port address is 780 and all other values are zero Select either Toggle P1 Bits or AutoTest to test that each output is controlling the appropriate stimuli lights sound generators grid floor aversive stimulus generator and door in the shuttle box The AutoTest option automatically progresses through each of the outputs Use the Toggle P1 Bits option to turn each output on and off one at a time If one or more of the outputs fails to properly control any of the stimuli verify that all of the cable connections are secure If they are and the problem persists contact Med Associates customer support for help The red and green LEDs on the SmartCtrl card in the interface cabinet correspond to each of the input and output buttons on this display When the AutoTest option is selected the outputs will illuminate one at a time in succession and the appropriate stimuli will switch on and off Figure 3 1 The MED Test Display MED Test Exit Standard Cards SuperPort SmartCtrl Pumps Misc Modules Window Help SmartCtrl DIG 716B DIG 703B 5 In Port Address In Offset Value w Out Port Address Out Offset Value Repeat Count BR
21. ds to be installed separately from MED PC IV Please refer to the User s Manual for MPC2XL for installation instructions Follow the step by step instructions below for importing data obtained from the shuttle box mpc procedures 1 Open Microsoft Excel and then minimize the window Open MED PC to Excel The MED PC to Excel display see Figure 6 1 will appear The leftmost file tab is titled Transfer Data shown in the figure below In the Table Transfer window at the bottom of the screen click Select Figure 6 1 Table Transfer MED PC to Excel BEI File Help Transfer Data Edit Row Profiles Edit Table Profiles r Row Transfer Profile NO PROFILE ASSIGNED r Transfer Orientation Column Labels Data Horizontal Rows JTraneten C Vertical Columns Table Transfer Profile NO PROFILE ASSIGNED lt r Transfer 34 Labels Bata Trraneten MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE 2 Choose Avoid MTP in the C MED PC IV Data folder Figure 6 2 Select Avoid Avoid MTP MED PC Table Profile El E 3 Note that now C MED PC IV Data Avoid MTP is listed under the Table Transfer Profile Select Labels and Data because selecting these options will print data labels as well as import data Then click Transfer Figure 6 3 Trans
22. e the duration of the entire session in minutes Dependent Variables Avoids The number of times out of the total number of trials per session that the subject has moved past the threshold and avoided the delivery of aversive stimulus Escapes The number of times out of the total number of trials per session that the subject experiences the aversive stimulus and then moves to the other side of the chamber is tallied under Escapes Crossings Movement from one side of the chamber to the other is registered as a crossing Passive Avoidance Training amp Testing Shuttle Box Passive Avoidance Training Level mpc amp Shuttle Box Passive Avoidance Test Level mpc Independent Variables Delay to Aversive Stimulus Onset on Dark Side sec This value defines the delay between when the subject enters the dark side of the chamber and when the aversive stimulus is administered The default value is 2 seconds Aversive Stimulus Duration sec The duration of aversive stimulus presentation and the default is 5 seconds No Response Time sec Conditioning has occurred once the subject fails to move into the more preferred dark side of the chamber The value used for No Response Time is thus a criterion with which to define whether conditioning has occurred The default is 180 seconds Inter Trial Interval sec The interval in between the offset of the aversive stimulus and the beginning of the next trial For shuttle box unit
23. eftGrid RightGrid gt S2 S6 0 5 gt 3 Punishment Recovery Period dz EndSession gt S1 Z Start CS gt 82 510 Start of Sequence with no Shock Wait for Crossing dz EndSession gt S1 2 EnterLeft Z EnterRight ADD D I 7 B TotalCrossings gt S11 Z Start CS S2 End of ITI S11 Time Delay to Match the with Shock Sequence 1 01 S10 dz EndSession gt S1 Z Start CS gt 82 DESCRIPTION OF STATE SETS 5 amp 6 State Set 5 calculates the response latency variables and prints the information in the data file array D State Set 6 prints the summary information to the MED PC Run Time Screen oko ko X RESPONSE LATENCY DETERMINATION Ke 5 6 5 S1 Z Start CS S2 52 Z Avoid SET B AvoidLatency B AvoidLatency D I 2 D I 4 0 gt S1 SET B EscapeLatency B EscapeLatency D I 4 D I 2 0 gt S1 Z Start ITI SET D I 2 0 D I 4 0 S1 No Response 0 01 SET D I 2 D I 2 0 01 Avoid Latency SET D I 4 D I 4 0 01 gt SX Escape Latency RH RER RER RER HR HR SHOW TRIAL AND SUMMARY DATA HR III TOTO TOR ITO ITO RER HRK S 8 6 S1 HSTART CLEAR 1 60 gt S2 52 1 SHOW 1 Trial B TrialCount 2 Avoid
24. ement and Display time only Session End is not triggered in 5 5 1 until Current Trial is completed 1 ADD E SHOW 5 Elapsed Time E 60 gt SX Z EndSession gt 51 55
25. er after the presentation of the stimulus The primary dependent measure includes whether the subject 1 escapes the aversive stimulus or 2 avoids the presentation of the aversive stimulus entirely by using the stimulus to predict its occurrence Passive avoidance includes both training conditioning and testing phases During training the subject is motivated to move to the preferred dark portion of the two compartment chamber The subject is then confined to the dark portion of the chamber and experiences inescapable aversive stimulus The subject is removed and placed back into the light portion of the chamber for testing During testing the subject is allowed free access to the dark side of the chamber and the latency to move into the dark side of the chamber is the primary dependent measure Increased latencies to enter the dark side of the chamber during the test are evidence If the inescapable aversive stimulus during the training phase was sufficient to produce conditioning then this should be evidenced by increased latencies to enter the dark side of the chamber during the test References Amorapanth P LeDoux J E Nader K 2000 Different lateral amygdala outputs mediate reactions and actions elicited by a fear arousing stimulus Nature Neuroscience 3 74 9 Caldarone B J Karthigeyan K Harrist A Hunsberger J G Wittmack E King S L Jatlow P Picciotto M R 2003 Sex differences in response to oral am
26. fer Data MED PC to Excel 220 MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE 4 Specify the raw data file to transfer and then click Open This step performs the transfer and now the data has been sent to Microsoft Excel Figure 6 4 Specify Data Files to Transfer Specify Data Files to Transfer Look in Data J ef E3 21 2005 02 14 Hippocampal MRP a 2005 02 15 Hippocampal MTP a 2005 02 16 2005 02 17 a 2005 02 18 a 2005 02 23 i2 24 Avoid MTP Avoidance MRP S SY Files of type m File 7 Network Open as read only 5 Expand the Microsoft Excel screen and the data should appear in the following format Figure 6 5 Excel Spreadsheet Microsoft Excel Book3 Eie Edi View Insert Format Tools Data Window Help D Eae T x85 o rz 5i c va 0 BruEssHB AT hd SUBJECT ID INFO Experiment Group Box Commen StartDate StartTime EndDate EndTime Total Trials Rat PHASE Hippocampal 1 1 2 24 2005 8 49 44 2 24 2005 8 51 40 5 INDEPENDENT VARIABLES Escape CS UCS Punish ITI Session Trials Stimuli Awoid Interval Interval Overlap Cross Time 5 3 500 2500 1 1 6 SUMMARY DATA Trials Escape Left Right Completed Avoid Avoid Lat Escape Lat Movement Movement Crossings ITI Shock Reserved 2 2 8 43 B1 92 7 TRIAL X TRIAL DATA Escape Left Right Tria
27. itriptyline in three animal models of depression in C57BL 6J mice Psychopharmacology 170 94 101 Miller N E 1948 Studies of fear as an acquirable drive I Fear as motivation and fear reduction as reinforcement in the learning of new responses Journa of Experimental Psychology 38 89 101 Mowrer O H amp Lamoreaux R R 1942 Avoidance conditioning and signal duration A study of secondary motivation and reward Psychological Monographs 54 Sidman M 1953 Avoidance conditioning with brief aversive stimulus and no exteroceptive warning signal 5 118 157 8 Active Avoidance Shuttle Box Avoidance Level mpc Independent Variables Trials to Run Define the total number of trials for the session A trial is terminated when the subject crosses the threshold between the two chambers to avoid or escape the aversive stimulus Therefore a trial consists of a CS tone light stimulus and possibly a UCS aversive stimulus presentation depending on whether the subject predicts the aversive stimulus occurrence or not The default is 50 Stimuli 1 tone 2 light 3 both The type of CS conditioned stimulus that will be utilized for avoidance conditioning The default value is 3 Avoid Interval sec The maximum duration of the CS tone light stimulus presentation prior to the delivery of the UCS aversive stimulus The default value is 5 sec If the CS UCS Overlap 0 see below And the subject
28. l Avoid Avoid Lat Escape Lat Movement Movement Crossings ITI Shock Reserved 1 0 0 1 131 2 1 0 2 0 0 1 20 3 1 0 0 3 0 0 1 5 9 4 51 1 0 4 1 27 0 0 31 9 2 1 0 5 1 17 0 0 2 9 1 0 0 Ready CAPS NUM 21 Editing the MTP file The MTP file can be edited to customize the transfer process and display the data of most interest See the User s Manual for MPC2XL for explicit instructions about how to modify the MTP file using the Edit Table Profiles screen see Figure 6 6 Header Titles are user defined and can include any information that will help label the data listed below the title Header Elements are the data points that will get transferred from the raw data file into Excel The raw data file will list the elements that can be included in the MTP file e g A Z Figure 6 6 Edit Table Profiles MED PC to Excel OE x File Help Transfer Data Edit Row Profiles Edit Table Profiles Edit Mode 1 3 2 E 6 4 Header Titles Subject Experimer Group Comment StartDate StartT Header Elements Subject Experimer Group Box Comment StartDate IStart INDEPEN Header Titles Trials Stimuli Avoid Inter Escape In CS UCS C Punish ITI Sessii Header Elements 0 1 A 2 A 3 A B SUMMAR Profile in Editor CAMED PC IM Data A
29. ng the filename and date created by the MED PC IV load wizard Figure 4 5 Data can be saved manually by selecting File Save Data Manually or File Save Data Flush Unless otherwise specified data will be saved to C MED PC IV Data Within each data file the headings are created for each Subject Experiment Group Box etc see below Data files may be opened with note pad word pad or any word processor or spreadsheet however make sure they are always saved unformatted in case you would ever want to use a data extraction utility such as MED PC to Excel Data file formats are explained in detail in the MED PC IV User s Manual File C MED PC IVIDATAV 2005 02 18 Start Date 02 14 2008 End Date 02 14 2008 Subject 1 Experiment PHASE 1 Session identification information Group Hippocampal 1a that was entered during the load Box 1 wizard see Figure 4 5 Start Time 9 43 05 End Time 10 40 16 MSN Shuttle Box Avoidance Level C 0 00 0 00 0 00 0 00 Elapsed Time in seconds Subscript for data array D See E below 0 00 N 0 00 E 0 00 L Animal Location Flag P 0 00 Q 0 00 X The current ITI drawn from List R 0 00 S S List of ITI times from 7 5 T 0 00 22 5 seconds with a mean 15 U 0 00 seconds see below for S array V 0 00 W 0 00 K m c N 0 5 00 3 00 500 00 2500 00 1 00 Named Va
30. periment Shuttle Box Level 1 Group Group 1 Comment Shuttle Box Level Experiment Filename SBLevel Subjectl Groupl Click Next to finish loading the box or Previous to correct errors 12 MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE The Alter Session Parameters Screen is the next screen to appear and is an important screen for the researcher The Alter Session Parameters screen allows the researcher to alter the parameters by which a procedure executes Click Next to proceed Figure 4 7 Alter Session Parameters Screen Number of Trials to Run Stimuli I2 Tone 2 Lgt 3 Both Avoid Interval sec Escape Interval CS UCS Overlap 12 Yes 0 No Punish ITI Cross 12 Yes 0 No Session Time min The Send Start Command Screen appears next The options available on the screen vary depending upon how many boxes are described in the Hardware Configuration In this example only 1 box is described in the Hardware Configuration so Figure 4 8 will appear next If more than 1 box is in the Hardware Configuration then Figure 4 9 will appear Figure 4 8 Send Start Command Screen for Single Box Configuration 13 MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE Figure 4 9 Send Start Command Screen for Multiple Box Configuration In both cases this is where the researcher decides to either load more boxes send a start signal to boxes that are already loaded or enter the M
31. pt for Data Array D L Location Flag 1 Left 2 Right S List of ITI times 7 5 22 5 seconds mean 15 seconds X This list may be edited as needed These numbers appear N in MED time units i e 1 second 100 MED Ticks given N a Resolution setting of 10 ms X Trial ITI Drawn from List S Z Pulses Used in This Procedure Start CS 1 Z1 Signal Start of CS Avoid 2 Z2 Signal Avoid Escape 3 NV Z3 Signal Escape Start ITI 4 Z4 Signal Start of ITI EnterLeft 5 NV Z5 Entrance to Left Compartment must break beam 2 4 EnterRight 6 NV Z6 Entrance to Right Compartment End CS E Z7 Signal CS UCS Overlap Stim Off EndSession 8 Z8 End of Session One 21 721 Beam 1 Break Two 22 722 Beam 2 Break Three 23 723 Beam 3 Break Four 24 724 Beam 4 Break Five 25 725 Beam 5 Break Six 26 Z26 Beam 6 Break Seven 27 727 Beam 7 Break Eight 28 Z28 Beam 8 Break Dimension Array Sizes DIM A 6 Named Variables DIM B 9 Summary Data Array DIM D 6000 Trial Data Array Available ITI Intervals Mean 15 Seconds DIST S s TEM ee LS 16 5 8T 19 5 2227220 DISKCOLUMNS 10 DISKFORMAT 6 2 DESCRIPTION OF STATE SET 1 State Set 1 is the core of program and sets the default values for all independent variables S1 programs the load wizard setup application S2 starts the procedure on animal ent
32. riables Array 5 1 00 6 00 Trials to run 5 Stimuli 3 Avoid Interval 5sec Escape Interval 25sec CS UCS Overlap 1 Punish ITI Cross 1 Session Time 6min 0 5 00 2 00 3 45 3 00 9 80 Data 54 102 00 52 00 10 00 5 00 0 00 Avoids Escapes Crossings D 1 D Trial by Trial Data 0 1 00 0 00 0 00 1 00 12 20 D I Trial Number 5 0 00 24 00 1 00 0 00 0 00 D I 1 Avoid 10 2 00 1 00 4 50 0 00 0 00 D I 2 Avoid Latency 15 27 00 1 00 1 00 0 00 0 00 0 1 3 Escape 20 3 00 0 00 0 00 1 00 8 70 D I 4 Escape Latency 25 45 00 4 00 2 00 1 00 0 00 D I 5 Left Movement Activity 30 4 00 0 00 0 00 1 00 8 50 2 D I 6 Right Movement Activity 35 19 00 19 00 3 00 2 00 0 00 40 5 00 1 00 2 40 0 00 0 00 crossings 45 11 00 11 00 4 00 3 00 0 00 0 1 8 ITI Aversive Stimuli D I 9 RESERVED 5 S List of ITI times 0 750 0 900 0 1050 0 1200 0 1350 0 7 5 22 5 seconds mean of 5 1500 0 1650 0 1800 0 1950 0 2100 0 15 sec 10 2250 0 These numbers appear in MED time units 1 sec 100 MED time units given a resolution setting of 10 ms Session 7 Comments see Figure 4 5 CHAPTER 6 Data Analysis Using MED PC to Excel Using a Pre Formatted Table Profile MTP file MED PC to Excel MPC2XL is a program that helps to import data from MED PC the raw data file format previous section to a spreadsheet program such as Microsoft EXcel MPC2XL nee
33. ry S3 ends the session S4 amp S5 repeats each trial S6 and saves the data S10 12 oko ko oko ko X Shuttle Box Avoidance Level Schedule 81 Set Default Values N Number of Trials to Run 50 N Stimuli Light amp Tone N Avoid Interval 5 seconds N Escape Interval 25 seconds X CS UCS Overlap Yes N Punish ITI Cross Yes X Session Time 60 minutes A IOI IO TORII IIIT ITO ITO ITO ITO ITO S S 1 Setup Adaptation amp ITI Interval Timers S1 0 001 SET A Trials 50 A StimTag 3 A CS Time 5 SET A UCS Time 25 A OverlapTag 1 A ITIShockTag 1 SET A SessionTime 60 S2 S2 5 SET A CS Time A CS Time 1 SET A UCS Time A UCS Time 1 SET B TrialCount 1 SET D I B TrialCount SET D I 10 987 987 RANDD X S ON Door Z Start CS gt 53 1 SHOW 1 Trials A Trials 2 Stimulus A StimTag 3 Avoid Time A CS Time SHOW 4 Escape Time A UCS Time 5 CS UCS Overlap A OverlapTag 6 ITI Shock A ITIShockTag SHOW 7 Session Time A SessionTime SX S3 Verify Detection of Animal 0 01 IF L 1 OR L 2 GStartNow Wait GStart Z Start CS S4 Wait gt SX S4 Wait for End of Trial Z Avoid Z Escape Z Start_ITI gt S5 S5 Time ITI Interval End Session following completion of ITI if Session Time or N
34. s B TotalAvoids SHOW 3 Escapes B TotalEscapes 4 Crossings B TotalCrossings SHOW 9 ITI Shocks B TotalITIShocks 11 Left Movement B LeftMovement SHOW 16 Right Movement B RightMovement gt SX dz EndSession S1 DESCRIPTION OF STATE SETS7 amp 8 State Sets 7 and 8 print avoid and escape latency data to the Run Time Screen TOR I TOR TORI TOR ITO IAC dO K Ke SHOW MEAN AVOID LATENCY RR TR TR TT TOR TOR TOR TOR TOR RH AK KK Ke S S T7 Sl START S2 82 1 IF B TotalAvoids gt 1 gt S3 S3 1 SHOW 7 Avoid Latency B AvoidLatency B TotalAvoids SX dz EndSession S1 SHOW MEAN ESCAPE LATENCY ko ko ko ko ko koe ko koe kk 5 5 8 51 HSTART gt 52 52 1 IF B TotalEscapes gt 1 gt S3 S3 1 SHOW 8 Escape Latency B EscapeLatency B TotalEscapes SX dz EndSession S1 DESCRIPTION OF STATE SET 9 State Set 9 operates the tone and light stimuli Whether the tone 1 the light 2 or both stimuli 3 function to promote avoidance are defined in the experimental load wizard Figure 4 7 S6 waits for either the escape avoid response or the end of the escape interval to turn off the stimuli STIMULUS CONTROL
35. s with automatic guillotine doors the opening of the door signals the end of the Inter Trial Interval The default is 60 seconds Dependent Variables Activity The number of I R photobeam interruptions in the light side of the chamber is the measure of Activity Latency sec The dark side of the chamber is the more preferred side but this is also where the animal experiences aversive stimulus The animal is going to be more reluctant to move into the dark side of the chamber with increased exposure to aversive stimulus Thus the Latency to move into the dark side of the chamber is a measure of conditioning NOTE Passive avoidance testing differs from training in that no aversive stimulus is administered during testing During testing the subject should be removed after it has moved into the dark side of the chamber The latency to move into the dark side of the chamber on the first trial is the most commonly used dependent measure MED ASSOCIATES INC SHUTTLE BOX AVOIDANCE CHAPTER 2 Hardware The hardware components below are included in the Deluxe Shuttle Avoidance Test Package manufacturer part number MED APA D1R e ENV 010MC Shuttle Box Chamber e ENV 018V Sound Attenuating Cubicle e Photobeam Sensors e Stimulus Lights e Tone Generators e Stainless Steel Grid Floor e Automatic Guillotine Door e ENV 410 Aversive Stimulus Generator 506 6080 Tabletop Cabinet e DIG 716B SmartCtrl Card DIG 700F De
36. sion S6 S3 Check to see if any Left Side Beams are being broken if not then count it as a crossing to the Right Side HR LeftIR 4 amp 4R LeftIR 3 amp 4R LeftIR 2 amp 4R LeftIR 1 S2 0 02 SET L 2 Z EnterRight gt 84 Z EndSession gt S6 54 Animal on Right Grid wait for Beam break on Left Side HR LeftIR 1 amp R LeftIR 2 R LeftIR 3 R LeftIR_4 gt S5 Z EndSession S6 S5 Check to see if any Right Side Beams are being broken if not then count it as a crossing to the Left Side HR RightIR 1 amp R RightIR 2 R RightIR_3 R RightIR_4 gt S4 0 02 SET L 1 Z EnterLeft gt 82 Z EndSession gt S6 olding ate a nd o ession S6 Holdi Stat t End of S i 1 gt SX DESCRIPTION OF STATE SET 3 State Set 3 controls the trial sequence for when the subject starts the trial on the left S1 S5 S8 and right S10 S13 side of the chamber X TRIAL SEQUENCE AND DATA COLLECTION ko ko ITO ITO RH Ke 6 6 3 51 Initiate Trials Z Start CS IF L 1 LeftTrial GNext LeftTrial gt S5 Next IF L 2 RightTrial Error RightTrial gt S10 GError gt SX S5 Trial Sequence Starting from Left Chamber CS Period A CS_Time T ON LeftGrid IF A OverLapTag gt 1 OverLap NoOverLap GOverLap S6 NoOverLap Z End CS
37. umber of Trials completed is equal to or greater than the set values IF E 60 gt A SessionTime OR B TrialCount gt A Trials EndSession NewTrial End Z EndSession gt S10 New gt S6 S6 Set up new Trial 0 01 ADD B TrialCount SET I I 10 SET D I B TrialCount D I 10 987 987 SHOW 1 Trial D I RANDD X S Z Start CS gt 54 510 End of Session Calculations Check for Zero Avoids 0 01 IF B TotalAvoids 0 True False True gt S11 False SET B AvoidLatency B AvoidLatency B TotalAvoids gt S11 S11 Check for Zero Escapes 0 01 IF B TotalEscapes 0 True GFalse True S12 False SET B EscapeLatency B EscapeLatency B TotalEscapes gt 512 512 Delay for Screen Update 2 OFF Door STOPABORTFLUSH DESCRIPTION OF STATE SET 2 State Set 2 tracks the location of the animal in the chamber S1 S3 N TRACK ANIMAL LOCATION ICI IO k K K dO K K 5 5 2 Set Location Flag L and issue Location Z Pulse S1 HR LeftIR 1 amp R LeftIR 2 R LeftIR 3 R LeftIR 4 SET L 1 S2 HR RightIR 1 amp R RightIR 2 R RightIR_3 R RightIR_4 SET L 2 54 S2 Animal on Left Grid wait for Beam break on Right Side R RightIR_1 amp R RightIR 2 R RightIR_3 R RightIR_4 gt 53 Z EndSes
38. void MTP Create Table Create Records Create Freestyle Open Save 2 To edit either the Header Titles or Header Elements click on the appropriate cell in the Edit Table Profiles window Rows and columns can be added to the file First select the desired location then right click to add either the desired row or column Use the right click option titled Paste an Identifier to include subject or session identifying information Note that when using the Paste an Identifier function Header Titles and Header Elements are edited and pasted automatically To save the edited MTP file select Save and create a new filename under the C MED PC IV Data folder To use this newly edited and saved MTP file verify that the file is selected in the Table Transfer Profile display Figure 6 3 and then click Transfer CHAPTER 7 Modifying the Existing Program Introduction Shuttle Box Avoidance Level mpc The Shuttle Box Avoidance procedure was written to run standard shuttle avoidance for a pecified number of trials default 50 Each trial produces a printout optional and data entry with the trial number avoid tag 1 avoid 0 no avoid avoid latency to the nearest 0 1 seconds escape tag escape latency left activity right activity crossings and ITI aversive stimuli Shuttle Box Avoidance Level mpc is listed below in its entirety S followed by a brief explanation of each state set See the MED PC IV User s
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