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Biomek FX and FXp Laboratory Automation Workstations

1. Install Cancel Figure B 32 New Devices Biomek FX and FX P Laboratory Automation Workstations User s Manual B 26 Using a Stacker Carousel 4 Check Stacker Carousel and choose Install The device is displayed under Stacker Carousels in the left pane Note The first Stacker Carousel added to the instrument is named Stacker1 the second is named Stacker2 and so forth OR Right click Stacker Carousel and choose Add Device gt Stacker Carousel The device is displayed under Stacker Carousels in the left pane Figure B 33 Biomek Hardware Setup p Reconnect ff Home All Axes h Add Device S Remove Device Y Accept gt lt Cancel Biomek FX SN None i AccuFrame 96 Podi 8 Pod2 f Devices 6 DeviceControllerO 6 ShakerALPO g7 ShakerALP1 47 SpeedPumpo oF StirrerALPO oF StirrerALP1 47 TiploaderO 47 Tiploader1 f Digital Devices E Simulator OP Camera g Materials Stacker Carousel Reconnect Figure B 33 Adding a Stacker Carousel Beckman Coulter Inc Using a Stacker Carousel B 27 B 4 2 Configuring Stacker Carousels To configure Stacker Carousels 1 In Hardware Setup select the desired stacker under Stacker Carousels in the left pane of Hardware Setup and the configuration view appears in the right pane Figure B 34 Biomek Hardware Setup Reconne
2. Shoulder 1 Figure 2 13 Sequence for installing four plunger screws and four shoulder screws on the HDR Tool Body 11 After all four shoulder screws are installed go back and tighten them securely in the same order 12 Replace the bottom pod cover and tighten the three screws 13 Turn on the power to the Biomek FX instrument 14 Retract the gripper using Advanced Manual Control Note After installing the HDR Tool Body on a Multichannel Pod Hardware Setup must be updated refer to Section 4 3 Configuring a Multichannel Pod Beckman Coulter Inc Multichannel Pod 2 19 2 4 Gripper The Multichannel Pod incorporates a gripper tool gripper Figure 2 14 that grasps and moves labware from one location on the Biomek FX deck to another The gripper can move labware no taller than 2 35 inches in height e move stacks of standard height labware no more than four plates high maximum 2 35 inches Note The gripper stacks and unstacks groups of labware from the bottom If a stack containing four pieces of labware is being unstacked the gripper grasps the top three and moves them to a new position Then the gripper grasps the top two of that stack and moves them finally the gripper grasps the top piece of labware in that stack and moves it to a new location e place lids on and remove lids from labware
3. BiomekFx Figure 4 6 Hardware Setup displaying Enable Plate Sensor An error message appears if labware is not sensed when the gripper is squeezed Figure 4 7 Refer to the Biomek Software User s Manual Chapter 26 Handling and Preventing Errors for information on recovering from errors Note By default Enable Plate Sensor is not checked Biomek Software Error in Move Labware from P3 to Orbital Eror during Assert labware gripped There was no plate sensed by the grippers j Retry Snap Figure 4 7 Error for no plate sensed 2 17 2004 11 05 22 AM Biomek FX and FX P Laboratory Automation Workstations User s Manual Configuring the Biomek FX in Hardware Setup Configuring a Span 8 Pod Configuring a Span 8 Pod in Hardware Setup includes Beckman Coulter Inc e Configuring the Probes for Fixed or Disposable Tips Section 4 4 1 e Configuring a Speed Pump Section 4 4 2 e Correlating the Pods Section 4 4 3 Note Correlating the pods should be performed only by a Beckman Coulter Service Engineer at initial setup e Correlating the Z Axes Section 4 4 4 Note Correlating the Z axes should be performed only by a Beckman Coulter Service Engineer at initial setup e Performing Find LLS Sensitivities Section 4 4 5 Performing Clot Detection Sensitivities available only with a Biomek FX Instrument Section 4 4 6
4. Movement Vector x fo cm Y fo om Z fo cm D fo cm M AutoClear 2 Clear Speed 100 Go stop Figure 5 23 Advanced Manual Control for moving gripper during framing Note Do not select Close if Advanced Manual Control closes the framing process must be completed again beginning with step 1 Manually move the pod into gripping position and hit OK Figure 5 24 Moving pod into gripping position Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 26 Framing the Biomek FX 8 Use Advanced Manual Control to move the gripper fingers until they are aligned with the bottom of the AccuFrame Figure 5 25 and squeeze the gripper fingers until they firmly touch the AccuFrame inside the notches at the bottom of the AccuFrame Figure 5 26 If necessary refer to Chapter 6 Manually Controlling the Biomek FX in Biomek Software Note Both front and rear gripper fingers must touch at the same time when squeezing which usually necessitates a move along the Y axis Note Assessing the position of the gripper fingers in step 7 is a visual task Figure 2 19 Figure 5 25 Gripper framing position on the AccuFrame Correct Gripper Alignment ncorrect Gripper Alignment Bottom of gripper finger aligned Top of gripper finger aligned to to bottom of AccuFrame and top of cut out of AccuFrame aligned to the inside o
5. A S D HE Gripper shaft Gripper shaft Gripper fingers Figure 2 14 Gripper The gripper contains two fingers e a double gripper located to the front of the instrument e a single gripper located to the back of the instrument Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 20 Multichannel Pod 2 4 1 Framing the Gripper with the AccuFrame f WARNING Gripper fingers may bend if not taught framed properly The gripper is framed during installation by a Beckman Coulter Service Engineer The framing process for the gripper must be repeated when A head is changed Extraordinary circumstances occur such as accidentally bending a gripper Should the gripper need to be framed refer to Section 5 4 Framing the Gripper on a Multichannel Pod 2 5 Preventive Maintenance The Multichannel Pod requires little preventive maintenance however to ensure optimum operation perform the following maintenance procedures as necessary f WARNING To prevent injury use proper decontamination procedures Beckman Coulter Inc Wipe up all spills immediately Return heads to their original packaging when they are not in use Check connections periodically to make sure that all are secure refer to Section 2 6 Troubleshooting Check and tighten head mount screws and gripper mount screws Make sure that gripper shafts are straight and gripper pads are i
6. I fe Fuses Fuse 2 4 carrier i Re i S2 Fuse carrier cover O 9 Figure 1 6 Replacing fuses in tower ae 22 e e ee T a z Biomek FX Introduction 1 15 4 Gently pull old fuses from the carrier by hand 5 Gently place new fuses into carrier by hand Note Use 250v 3 15 amp 5x20 slow blow type fuses for replacement 6 Replace the fuse carrier into the AC power input module 7 Plug the instrument into the power source 8 Turn on the instrument power switch Biomek FX and FX P Laboratory Automation Workstations User s Manual Multichannel Pod 2 1 Overview The Multichannel Pod is a self contained working unit installed on the right left or both bridges of the Biomek FX Figure 2 1 The Multichannel Pod is a full microplate replication tool incorporating a gripper and interchangeable heads to accommodate a variety of functions refer to Section 2 3 Interchangeable Heads The Multichannel Pod contains its own electrical cabling communication and pneumatic connections with the base unit and it interacts with ALPs located over the entire deck area of the Biomek FX Note When installed on a dual pod system the pod mounted on the left side of the Biomek FX instrument is only capable of accessing ALPs on that side of the deck while a p
7. Always move to max Z when roving Check this field to move the pod to its maximum height during any move in the X or Y axis Enable Plate Sensor Enables the plate sensor on the gripper to sense labware Default setting is not checked Refer to Section 4 3 3 1 Using the Plate Sensor Final D Position under Tip Load Settings Position the pod returns to along the D axis after loading tips Final D Position under Tip Unload Settings Position the pod returns to along the D axis after unloading tips Gripper Extend Time Specifies the time the pod takes to extend grippers Gripper Retract Time Specifies the time the pod takes to retract grippers Gripper X Y Z D Offsets Controls gripping operation along X Y Z and D axes from center of back edge of deck position These offsets are automatically updated when the gripper is framed Note Gripper X offset is read only Last Validation Set by a Beckman Coulter Service Engineer using the Set Validation button Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 10 Configuring the Biomek FX in Hardware Setup Table 4 2 Multichannel Pod Properties Continued Property Description Maximum X Y Z D The maximum position the pod may move to along the X Y Z and D axes relative to the Home position Set by using the appropriate buttons under the X cm Y cm Z cm
8. Figure 4 34 Confirmation to reset stored configuration 5 Choose Yes The stored configuration is reset Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 36 Configuring the Biomek FX in Hardware Setup Beckman Coulter Inc 4 5 3 To delete settings Deleting Pod Settings 1 In Hardware Setup select the appropriate Pod from the left pane to access the configuration area in the right pane Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 2 Choose Delete Settings Delete Settings appear Figure 4 35 Delete Settings Settings Settings Information MultichannelPodS ettings1 Validation Time Not Validated a Serial Number Head Type 96 Mandrel 200 pL Head Select desired settings Y 590 3320 to delete here D 58 43 229 00 Speed Limit 100 Additional Roving Height o 75cm Additional Timeout 25 00 Do not move to Z max men roving Gripper Settings Gripper Offset zi coea Figure 4 35 Delete Settings 3 Select the desired settings to delete 4 Choose OK The following Confirm appears Figure 4 36 i Confirm Q Are you certain you want to delete the stored configuration MultichannelPodSettings1 Click Yes to delete MultichannelPodSettings1 Click No to return to Hardware Setup without deleting Figure 4 36 Confirmation to delete stored configuration 5
9. Note Some ALPs and devices require a Device Controller Section 1 2 4 3 to provide power and communications between the device and the Biomek FX instrument 1 2 4 1 ALPs Automated Labware Positioners ALPs are removable and interchangeable platform structures that are installed on the Biomek deck to allow automated assays to be performed ALPs are either e Passive ALPs some hold labware in place on the deck while others act as a receptacles for by products from methods such as system fluid and disposed tips tip boxes and labware OR e Active ALPs contain mechanisms that may hook to power and or air sources for mechanical operation such as tip loading tip washing mixing stirring shaking and precisely positioning labware Note Refer to the ALPs User s Manual for detailed information on all available ALPs for the Biomek FX 1 2 4 2 Optional Devices and Solutions Optional devices and solutions such as a Stacker Carousel can be integrated with the Biomek FX instrument to accommodate specific operations Note Refer to Appendix B Using a Stacker Carousel for information about integrating a Stacker Carousel 1 2 4 3 Device Controller A Device Controller is a peripheral box that connects to the Biomek FX to provide a means to control a number of high voltage 110 VAC 220VAC and low voltage 24VDC devices and low voltage digital inputs High voltage devices receive power through an AC Power In
10. 0 03 0 02 in 0 03 0 02 in Wide side Narrow side Figure B 31 Label positioning on microplate Beckman Coulter Inc Using a Stacker Carousel B 25 B 4 Configuring the Stacker Carousel in Hardware Setup Use Hardware Setup to install configure and remove Stacker Carousels Refer to the Biomek FX and FX P Laboratory Automation Workstations User s Manual Chapter 4 Configuring the Biomek FX in Hardware Setup for more information on Hardware Setup Hardware Setup is accessed from within Biomek Software B 4 1 Installing Stacker Carousels To install Stacker Carousels 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software Biomek Software appears 2 From the Instrument menu choose Hardware Setup Hardware Setup appears 3 Choose Add Device gt Stacker Carousel New Devices appears Figure B 32 New Devices Available Devices iFan MagBead LP HW Address 00 MagBead LP HW Address 01 MagBead4LP HW Address 02 MagBeadA amp LP HW Address 03 OrbitalShaker4LP Hw Address 00 OrbitalShaker4LP Hw Address 01 _ OrbitalShakerALP Hw Address 02 OrbitalShakerA4LP Hw Address 03 PositivePosition4LP HW Address 00 PositivePositionSLP Hw Address 01 Simple SpeedPump Hw Address 01 Stacker Carousel Tiploader HW Address 2 Tiploader Hw Address 3 WashPump
11. s ssesessssessessessesseessessessees 3 38 Table 3 6 Parts and materials along the Span 8 Tip Wash ALP fluid flow Paths scsiccssciisnsiisosdaceseniedessashsstuseasaneesovacnterasssaunes savaeasedisndevenes 3 39 Sable 3 7 Speed Pump Specifications sa saniccssicwasasdnsxisnesutaventasedssmscsantossdeutasweddsueinestdeacesmunssavbes 3 49 Table 3 8 Span 8 Pod Troubleshooting cccsceussssasenasnasssesaecsnanseessvenssausnesnsnassenseatannassensvensaenss 3 54 Table 4 1 Hardware Setup Options lt csvsiesuniassncnsiinineaeie 4 3 Table 4 2 Multichannel Pod Properties s nsnsnneneseessessenssessesseesseesessrnsseeseesesssreseesenssreseesersseesne 4 9 Table 4 3 Span 8 Pod Properties xid sidsssasnedassasasncexeaiasccaaasdteava dsanccassstaccinssatucasbatneussbisaewaaasancaes 4 32 Table 4 4 P rge SUNG vgn sce stots besciancyu e e R E E E EAA 4 33 Table 5 1 Troubleshooting Framing s ssseesssssessesessessseesreseesstesrsresstessesersstessessessressessrssres 5 28 Table 6 1 Advanced Manual Control selection areas for the Multichannel Pod 0 6 8 Table 6 2 Advanced Manual Control selection areas for the Span 8 Pod cece eeeeeeeeee 6 16 Table A 1 System Speci ficati NS isisi siisii nra R EE RN E AEE ata eaters A 1 Biomek FX and FX P Laboratory Automation Workstations User s Manual Biomek FX Introduction Overview Note Unless otherwise noted all information in this hardware manual refers t
12. Cancel Figure 5 2 Warning to address before homing process begins 3 Choose OK on each Warning and Information after confirming that the actions have been addressed appropriately 5 2 2 Attaching the Framing Fixture to the Pod After homing the pod s the appropriate framing fixture must be installed on the pod used for framing The type of framing fixture required depends on the type of pod and head installed e Multichannel Pod with a 96 channel or 384 channel head refer to Section 5 2 2 1 Attaching the Framing Fixture to the 96 Channel or 384 Channel Head of a Multichannel Pod e Multichannel Pod with the HDR Tool Body refer to Section 5 2 2 2 Attaching the Framing Fixture to the HDR Tool Body of a Multichannel Pod e Span 8 Pod refer to Section 5 2 2 3 Attaching the Framing Shaft to the Span amp Pod Beckman Coulter Inc Framing the Biomek FX 5 5 5 2 2 1 Attaching the Framing Fixture to the 96 Channel or 384 Channel Head of a Multichannel Pod When positioning the framing fixture the framing probe must be pointed down and away from the mandrels of the head From the front perspective the framing guides on the framing fixture must be to the back and left To install the framing fixture on the multichannel head 1 Hold the framing fixture Figure 5 3 against the head with the framing guides pressed to the outside of the back row and left column of mandrels Note M
13. Beckman Coulter Inc Span 8 Pod 3 21 3 3 6 Disposable Tip Mandrels Disposable tips can be used on the Multichannel Pod or the Span 8 Pod but LLS disposable tips should be used only on the Span 8 Pod To use the disposable tips option disposable tip mandrels must be installed on the Span 8 probes and configured for use in the Hardware Setup refer to Section 4 4 Configuring a Span 8 Pod 3 3 6 1 Installing Disposable Tip Mandrels CAUTION Do not perform aspirate or dispense operations without mandrels installed and tubing attached to disposable tips To operate the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod gt e Note A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tip mandrels on the Span 8 Pod refer to Section 3 3 4 Electro Static Discharge Protection when Changing Tips on the Span 8 Pod To install disposable tip mandrels 1 Place the end of a disposable tip mandrel into the tubing that extends from the bottom of the tip interface Figure 3 13 Probe Tip Interface 4 Disposable Tip Mandrel Figure 3 13 Inserting a disposable tip mandrel into the tubing Biomek F
14. The sections in this chapter include e Using Manual Control Section 6 3 e Using Advanced Manual Control with the Multichannel Pod Section 6 4 e Using Advanced Manual Control with the Span 8 Pod Section 6 5 e Using Advanced Manual Control to Manually Control Speed Pumps Section 6 6 Biomek FX and FX P Laboratory Automation Workstations User s Manual 6 2 Manually Controlling the Biomek FX in Biomek Softwa i 6 2 Accessing Manual Control To open Manual Control choose Instrument gt Manual Control An Information dialog Figure 6 1 appears briefly as the connection is made with the Biomek FX instrument immediately followed by Manual Control Figure 6 2 Note Manual Control is available only when a method is not being executed If a need for manual control is realized during a method run stop the method using the Stop button or Snap Continuation button refer to the Biomek Software User s Manual Chapter 26 11 2 Snapping a Continuation on the toolbar before accessing Manual Control Information x G Connecting Manual Control ose a He Advanced Controls Home All Axes Get version Figure 6 2 Manual Control Beckman Coulter Inc 6 3 ally Controlling the Biomek FX in Biomek Software 6 3 Using Manual Control Use Manual Control for e Homing all Axes of the Pod or Pods Section 6 3 1 e Moving a Pod to a Specific Deck Position Section 6 3 2
15. 8 Pod2 Devices IV This is a dual armed system 7 DeviceControllerO 47 ShakerALPO Left Pod Type Left Multichannel Pod 7 oF Shaker LP1 oF SpeedPump0 Right Pod Type Right Span 8 Pod 47 SpeedPump1 oF StirrerALPO oF StirrerALP1 oF TiploaderO 47 Tiploader1 Digital Devices J WashPump1 a e Simulator e Camera E Materials f Stacker Carousels Figure 4 1 Hardware Setup for a dual pod system with a Multichannel Pod and Span 8 Pod Note A single pod system lists only the one installed pod Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 3 4 1 2 Understanding the Options in Hardware Setup An understanding of the options on the toolbar in Hardware Setup Figure 4 1 is necessary to properly install configure and remove devices Table 4 1 lists and describes the toolbar options in Hardware Setup Table 4 1 Hardware Setup Options Option Reconnect Description Allows Hardware Setup to reexamine the devices present Choose this option to determine what devices are present rather than closing and reopening Hardware Setup Home All Axes Gives the Biomek FX instrument a point of reference from which to make subsequent moves For a single pod system home position is left back For a dual pod system home position for the first left pod is left back and for the second right pod is right back Note Pods should
16. COULTER Biomek FX and FX Laboratory Automation Workstations User s Manual Beckman Coulter PN 987834 evision Beckman Coulter Inc Except as provided in writing signed by an officer to Beckman Coulter Inc this system and any related documentation are provided as is without warranty of any kind expressed or implied including that the system is error free This information is presented in good faith but Beckman Coulter Inc does not warrant guarantee or make any representations regarding the use or the results of the use of this system and related documentation in terms of correctness accuracy reliability currentness omissions or otherwise The entire risk as to the use results and performance of this system and related documentation is assumed by the user Except as expressly provided herein Beckman Coulter Inc makes no other warranty whether oral or written expressed or implied as to any matter whatsoever including but not limited to those concerning merchantability and fitness for a particular purpose nor is freedom from any patent owned by Beckman Coulter Inc or by others to be inferred LIMITATIONS OF LIABILITY Beckman Coulter Inc shall not be liable to any extent whatsoever for any damages resulting from or arising out of the use or performance of this system and related documentation or the procedures specified in this manual regardless of foreseeability or the form of action whether i
17. Fixed tips are available in two sizes with or without Teflon coating Disposable tip mandrels may also be installed on the Span 8 probes When using disposable tip mandrels any standard LLS capable or barrier disposable tips may be loaded onto and unloaded from the disposable tip mandrels Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 6 Span 8 Pod 3 3 1 Liquid Level Sensing If the liquid level in a piece of labware is not known the Span 8 Pod can determine the liquid level when Liquid Level Sensing LLS capable tips are attached to the probes Note LLS tips must be attached to the probes to detect the liquid level Note An LLS plate option used on a 1 x1 Passive ALP can improve LLS capabilities refer to the ALPs User s Manual Chapter 19 1 1 1 1 x 1 Passive ALP with LLS Plate Option LLS tips are used to determine the liquid level in a piece of labware by detecting a shift in the capacitance The LLS tip moves to a specified height within a well and then slowly moves down into the well When the tip contacts liquid there is a large change in the capacitance detected The liquid level is sensed by determining the height at which this change in capacitance occurs Refer to Section 6 5 6 Verifying Liquid Level Sensing for more information on detecting the liquid level in labware The Span 8 Pod on the Biomek FX supports the following liquid level sensing tips e Fixed 60 Coated and Uncoat
18. Note Clot detection is only available with a Biomek FX instrument e Setting Span 8 Pod Properties Section 4 4 7 as instructed by a Beckman Coulter Service Engineer e Enabling Purge Settings Section 4 4 8 by a Beckman Coulter Service Engineer Note The settings for a Multichannel Pod can be saved restored and deleted refer to Section 4 5 Saving Restoring and Deleting Settings 4 4 1 Configuring the Probes for Fixed or Disposable Tips The probes on the Span 8 Pod may be configured for fixed or disposable tips This configuration must be accomplished in Hardware Setup The probes on the Span 8 Pod must be configured when e Changing from disposable tips to fixed tips e Changing from fixed tips to disposable tips e Changing the type of fixed tips When probe configuration is changed from disposable to fixed tips or from fixed to disposable tips the pod must be homed refer to Section 6 3 1 Homing all Axes of the Pod or Pods and Find Sensitivities must be performed refer to Section 4 4 5 Performing Find LLS Sensitivities Note The pod may be homed by choosing Home All Axes from Hardware Setup Note The Span 8 Pod is capable of accessing all types of labware supported by the Biomek FX instrument however specific types of tips are recommended to access specific types of labware refer to Section 3 3 3 1 Labware and Tip Compatibility with the Span 8 Pod Configuring the Biomek
19. and D uL columns The D axis must be established when a head is changed but do not change the other axes limits without contacting a Beckman Coulter Service Engineer Minimum X Y Z D The minimun position the pod may move to along the X Y Z and D axes relative to the Home position Set by using the appropriate buttons under the X cm Y cm Z cm and D uL columns The D axis must be established when a head is changed but do not change the other axes limits without contacting a Beckman Coulter Service Engineer Speed Limit Controls the speed for pod movement based on a percent of its maximum speed Tip Settle Time Specifies the time required for tips to settle on the mandrels after loading Tip Unload Time Specifies the amount of time the pod takes to unload tips Unload Speed Controls the pod speed when unloading tips based on a percentage of the maximum pod speed Recommended that this be modified only by a Beckman Coulter Service Engineer 4 3 3 1 Using the Plate Sensor The plate sensor on the gripper is available on newer Biomek FX instruments To enable the plate sensor the Multichannel Pod must have e Master Controller firmware version 2 62 e ZD Board version 0 11 0 or later Note To verify the Master Controller and ZD Board versions refer to Section 6 3 4 Viewing the Firmware Version e Biomek Software version 3 0 or later Note To verify the software version c
20. 96 Podi Port Simulate z 8 Pod2 Devices IV This is a dual armed system DeviceControllerO ShakerALPO Left Pod Type Left Multichannel Pod 7 ShakerALP1 SpeedPump0 Right Pod Type Right Span 8 Pod z F SpeedPump1 F StirrerALPO eaten i List of Devices TiploaderO Tiploader1 EM Digital Devices E WashPump1 E Simulator Camera g Materials amp Stacker Carousels onal BiomekFX Figure 4 11 Hardware Setup Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 17 2 Select Add Device New Devices appears Figure 4 12 New Devices Available Devices TS peedPump Hi Address 00 C SpeedPump Hw Address 01 Install Cancel Figure 4 12 New Devices 3 Select SpeedPump HW Address 00 for the first Speed Pump added to the Biomek FX instrument Select SpeedPump HW Address 01 for the second Speed Pump added to the instrument and SpeedPump HW Address 02 for the third Note Only three Speed Pumps can be added to the Biomek FX instrument at one time Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 18 Configuring the Biomek FX in Hardware Setup 4 In New Devices choose Install to add the new Speed Pump to the Hardware Setup and close New Devices A Speed Pump has been added to the Hardware Setup Figure 4 13 Go to step 8 below to associate th
21. CAN interface with a small microcontroller provides power and communication to the Speed Pump e Two address switches e One fluid out port e One fluid in port e One fluid overflow port Fluid out Address Fluid in switches Overflow Figure 3 34 Speed Pump back view The Speed Pump is shipped in two pieces e The Speed Pump Figure 3 34 The tubing connector with the fluid in and overflow tubes already attached Figure 3 35 Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 50 Span 8 Pod 3 6 1 Speed Pump Specifications Table 3 7 Speed Pump Specifications Item Description Environment Indoor use only Input Power Requirements 36V supplied via the CAN bus Dimensions 7 52 wide x 6 04 deep x 5 91 high Weight 3 25 lbs Ambient Operating Temperature 5 30 C 41 86 F Humidity Restrictions lt 85 non condensing 30 C 86 F Speed Pump manufactured by Beckman Coulter Inc 4300 N Harbor Boulevard Fullerton CA 92834 3100 3 6 2 Adding a Speed Pump to the Biomek FX Instrument A Speed Pump can be installed anywhere convenient for normal operation of the Biomek FX instrument as long as it is positioned in line between the supply container and the pumps Installing the Speed Pump involves attaching the tubing connector to the Speed Pump and attaching the system
22. Choose Yes The stored configuration is deleted 5 1 Framing the Biomek FX 5 1 Overview Framing is the procedure of teaching Biomek Software the location of the ALPs and devices positioned on the deck Biomek Software uses this framing information to move the pod s to the appropriate positions to perform liquid handling operations and manipulate labware A Beckman Coulter Service Engineer frames the Biomek FX instrument during system installation It may be necessary to repeat the framing if e ALPs or devices are added moved or removed from the deck e the head on the Multichannel Pod is changed e the probes on the Span 8 Pod are changed Framing deck positions can be performed automatically using the AccuFrame framing tool or manually using a piece of labware to visually align the pod to the wells The gripper on the Multichannel Pod may also be framed manually using the AccuFrame framing tool Framing the Biomek FX instrument includes Framing Deck Positions on the Biomek FX Using AccuFrame Section 5 2 e Manually Framing Deck Positions on the Biomek FX Section 5 3 Framing the Gripper on a Multichannel Pod Section 5 4 5 1 1 Precision When Teaching Two Pods After framing with a pod Pod 1 for example Pod 2 Coordinates change to match those of Pod 1 The Precision field for Pod 2 however still displays Not Framed until Pod 2 is actually framed for that position When precision is c
23. FX in Hardware Setup 4 13 To configure probes 1 In Hardware Setup select the appropriate Span 8 Pod from the left pane to access the configuration area in the right pane Figure 4 8 Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 Biomek Hardware Setup E Reconnect Mf Home All Axes Gp Add Device me Remove Device J Accept XX Cancel Biomek FX SN None Probe Configuration Sae Sii ee eee fen estore Settings ete Settings r fj AccuFrame Click to select which probes to configure ava eee j nig z g 96 Podi Last Validation Not Validated Set Validation Time 8 Pod2 amp Devices Axis Limit Settings g7 DeviceController0 x cm cm Z cm D ul Span cm oF ShakerALPo a Minimum 23 79 fs 15 938 fo fe 3 7 ShakerALPt g7 SpeedPumpo Maximum 102 79 jaa 6 561658 fiooo jia oF SpeedPump1 Probes 1 4 use Set Set y paella Disposable 7 tips g Stirrer lP1 na aay ae z oF Tiploader0 fi mL 7 syringes Gorrelate Pods Correlate Z Axes Find LLS Sensitivities 7 Tiploader1 Disabled Probes Digital Devices 1 2 3 4 5 g 7 8 Pump lt None gt gt Find GD Sensitivities E Simulator E Camera Materials Additional Pod Settings Stacker Carousels Purge Settings BiomekFx Figure 4 8 Hardware Setup showing the configuration view for a Span 8 Pod 2 To choose the
24. Manual Teaching closes and Position Properties appears Figure 5 13 22 Choose OK to save the framing information and close Position Properties Note Both pods on a dual pod Biomek FX instrument must frame the same deck position 23 Repeat steps 3 to 22 to frame additional deck positions using manual teach 24 Choose Save to save framing information for all positions and close the Deck Editor Figure 5 12 Note Choosing Cancel loses all changes to the deck including framing information since the Deck Editor was opened Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 22 Framing the Biomek FX 5 4 Framing the Gripper on a Multichannel Pod A Beckman Coulter Service Engineer frames the gripper on a Multichannel Pod during system installation however it may be necessary to repeat the framing procedure if e the head on a Multichannel Pod is changed e gripper is replaced e extraordinary circumstances occur such as accidentally bending a gripper finger Before framing the gripper make sure instrument is homed refer to Section 5 2 1 Homing All Axes of the Pods e deck position is framed prior to framing the gripper to that location refer to Section 5 2 Framing Deck Positions on the Biomek FX Using AccuFrame e AccuFrame is installed on the appropriate position refer to Section 5 2 3 Installing AccuFrame e framing fixture is not attached to the Multichann
25. Tip Interface Fixed Tip Collar Figure 3 8 Passing collar over fixed tip Beckman Coulter Inc Span 8 Pod 3 17 5 Screw the collar clockwise onto the tip interface until a tight fit is achieved The tip is now installed Figure 3 9 6 Repeat steps through 5 for any other probes requiring fixed tips Probe Tip Interface Collar Fixed Tip Figure 3 9 Installed fixed tip Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 18 Span 8 Pod 3 3 5 2 Removing Fixed Tips WARNING SPILL HAZARD WARNING Use an appropriately contained environment when using hazardous materials WARNING Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety officer WARNING Observe cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials WARNING Always wear protective gloves when removing the tips CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod PEEP EE Note A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tips on the Span 8 Pod refer to Section 3 3 4 Electro Static Discharge Protection when Changing Tips on the Span 8
26. and connections Figure 1 3 e X axis linear rail travel plate for the bridges e Main power switch on the outside of the right tower e Communication hookup to host computer via RS232 on the outside of the left tower e Electrical plug ins and CAN connections for active ALPs on the inside and outside of both towers e Pneumatically regulated air supply ports on the inside and outside of both towers for active ALPs e Main power fuses for the instrument on the outside of the right tower Inside tower connections Left Right 6 O ro von on ont font o B o o g f o ZB 0p gaia es ie LB 3 RA f f RAA EN K K f A KT KAY WAY Ys ff A ff YK NA LF A Outside tower connections Left Right 6 ro gt C j O F gt r 3 a8 i Bo of S i S o ia o Main S 5 g wm ol switch wn XY o lt p Fuse E cm Most carrier PORT 7 DW dewo IA Y Y Figure 1 3 Inside and outside tower connections detail view Biomek FX and FX P Laboratory Automation Workstations User s Manual 1 6 Biomek FX Introduction 1 2 1 1 Indicator Lights A status indicator panel with green and amber indicator lights is built into the main cover and indicates the current operational status of the Biomek instrument and light curtain refer to Section 1 2 5 1 Light Curtain Protection System Table 1 1 defines the indicator light and the operational status each repr
27. crashing into the microplate CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when installing the HDR Framing Fixture WARNING Turn off power to the Biomek FX instrument before attaching or removing AccuFrame from the instrument deck WARNING Make sure the light curtain is not violated by the AccuFrame cable If the light curtain is violated the framing process halts immediately WARNING Make sure the AccuFrame cable does not interfere with pod movement CAUTION Use Shift deck only before other ALPs or positions are taught Shift deck shifts all ALPs and positions resulting in incorrect coordinates if applied to previously framed ALPs and positions CAUTION Always ensure that the gripper is retracted before homing CAUTION Select Move Z Max to move the pod to its highest point before extending the gripper To avoid breaking labware or bending the gripper fingers make sure the gripper will not hit any labware when extended Safety Information xiii CAUTION Do not retract gripper when it is holding labware WARNING CLASS II LASER PRODUCT THIS PRODUCT CONFORMS TO APPLICABLE REQUIREMENTS OF 21 CFR 1040 AT THE DATE OF MANUFACTURE CAUTION To avoid serious damage to the instrument make sure that the laboratory site voltage frequency matches the voltage frequency that was ordered for the instrument WARNING Do not attempt to remove or replace covers while the unit i
28. e Stopping a Pod Section 6 3 3 e Viewing the Firmware Version Section 6 3 4 e Accessing Advanced Manual Control Section 6 3 5 6 3 1 Homing all Axes of the Pod or Pods CAUTION Before selecting OK to home all axes the following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the gripper on the Multichannel Pod is retracted Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure that the two arms are not near each other at either end of the rail Home the pods each time the Biomek FX instrument is powered on Homing the pods gives Biomek FX a point of reference from which to make subsequent moves For a single pod system home position is left back For a dual pod system home position for the first left pod is left back and for the second right pod is right back Note When attempting to use the pod error messages result until the pod is homed Note While it is necessary to home the pods after the Biomek FX instrument is powered on it is not necessary to home the pods each time the host computer is turned on or the software is accessed Biomek FX and FX P Laboratory Automation Workstations User s Manual 6 4 Manually Controlling the Biomek FX in Biomek S f CAUTION Always ensure
29. pump assembly defined 3 28 purging air from the syringes and tubing 6 22 setting valve states 6 21 speed pump 3 47 syringes 3 29 tips supported 3 5 troubleshooting 3 54 using non supported system fluids 3 37 wrist ground strap when accessing tips 3 12 specifications Biomek FX system A 1 Speed Pump adding a 3 49 configuring 4 16 connections 3 48 defined 3 47 manually controlling 6 24 setting address switches 3 52 specifications 3 49 Stacker Carousel Biomek FX attaching alignment bracket B 6 attaching side shields B 8 attaching stacker B 8 framing B 29 integrating B 1 mounting B 5 operating B 12 positioning the B 3 supply container defined 3 37 3 40 replacing 3 41 syringes assembling 3 31 cutoff velocity 3 35 defined 3 29 disassembling 3 34 how size affects pipetting accuracy 3 30 how they affect pipetting accuracy 3 30 installing 3 31 optimizing life expectancy 3 36 optimizing performance 3 36 removing 3 34 sizes supported 3 30 system fluid defined 3 37 degassing 3 45 priming the system with 3 46 purging the tubing and syringes of air with 3 46 T teaching framing grippers 5 22 instructions 5 1 precision when teaching two pods 5 1 Stacker Carousel B 29 using Manual Teach 5 15 tower changing fuses in 1 14 electrical connections detail view 1 5 electrical connections instrument graphic 1 4 overview 1 4 troubleshooting 1 13 tubing defined 3 37 types required 3 39 V Vector Builder defined
30. workspace of the instrument Use Absolute Move when the coordinates of the desired position are known Note When an Absolute Move is entered the values displayed in Movement Vector reflect the relative move required to physically move the pod to the desired position Make sure the physical location of the pod is not changed between the time the vector is built and the time the Go button is pressed 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Multichannel Pod appears Figure 6 9 4 Choose Absolute Move Absolute Move appears Figure 6 11 Absolute Move Current Position x 1 092 em Y 0 1 em Z 3 95 cm D 219 379 cm Enter the absolute coordinates to move to em 4 0 1 em Z 3 95 cm D A 9 379 ub These values will be converted to the necessary relative move numbers on the Advanced Manual Control form when you click OK Then you can press Go to move to these coordinates Cancel Figure 6 11 Enter Absolute Move Coordinates 5 Enter the X Y Z and D values for the desired position Note Minimum and Maximum X Y Z and D values displayed in Hardware Setup indicate how far the pod is able to move These values are also displayed as a tool tip when hovering over the fields in Movement Vectors 6 Choose OK The Movement Vector changes to reflect the necessary relative move 7 Enter
31. 14 Absolute Move for Span 8 Pod svjejccscscesassaceaccasvnndseieuseieinsesavasarncieaeneins 6 20 Figure 6 15 Valve Seting S nessen a e A a EEE E D 6 21 Figure 6 16 Warning to address if a Span 8 Tip Wash ALP is not defined ee eeeeeeeees 6 22 Fig re 6 17 Informatio N ys ica gassesiasdsaccand secicavnarnceiaiadeasaadecaa adanne aan aii 6 23 Figure 6 18 Advanced Manual Control for a selected Speed Pump ccceccccesceseeseeeeeees 6 24 Figure B 1 Stacker Car Use sccoscanccunaceasaastatexnadinncen aceaqmenteancuwentcaweaesteneonenaeenterearniautenmcemeeaiens B 2 Figure B 2 Stacker shuttle positions c cscencsexsduczicccsseartansaencsasesacevacenncsdedascastavsseaseaciveateanewesentaves B 3 Figure B 3 Stacker Carousel integration s sssssesssesessseeseseesseesesrssstesttsrsessteseesensseessesensseesee B 4 Figure B 4 Right horizontal alignment bracket asisssssssvnsevadayvacedvonwiavsuatinarsusiwaaveaatuasadiannenciaanveiss B 5 Figure B 5 Left horizontal alignment bracket ssascyacecsdnvgstoersdaceancsnscuersanaussanseedevassacsagnsasiousnanes B 5 Figure B 6 Attach alignment bracket o sssseseesesseseeseeseeesssesetsessesersesseeersreerssesesesseseeseeseeessse B 6 Figure B 7 Attaching riser plate ssnessesssssesseessesssrseessessersoessesssesorsseeronssrssesseerorssrsseersesorsseeses B 7 Fig re B8 Stacker attachment sonnin A A B 8 Figure B 9 Plexiglas side shield cutout blank orientations s sssesss
32. 5 4 Waste Container 3 5 1 System Fluid System fluid is used to create a vacuum that facilitates the aspirate and dispense actions of the Span 8 Pod System fluid is also used to wash fixed tips positioned in the Span 8 Tip Wash ALP CAUTION Tap water is not supported due to high mineral content which AN could cause blockages in the tubing and increase the possibility of leaks at tubing connections The following liquids are supported as system fluid for the Span 8 Pod e De ionized water e Distilled water Note Itis possible to use system fluids other than those supported for use with the Span 8 Pod However the corrosive nature of each fluid and its impact on system components along the Span 8 system fluid flow path and in the Span 8 Tip Wash ALP must be considered 3 5 1 1 Using Non Supported System Fluids System fluids other than those listed as supported system fluids refer to Section 3 5 1 System Fluid may damage the components along the Span 8 system fluid flow path and the Span 8 Tip Wash ALP Therefore before using non supported system fluids use Table 3 5 to verify that component parts along the Span 8 system fluid flow path are not susceptible to the caustic nature of any non supported system fluids Use Table 3 6 to verify the parts on the Span 8 Tip Wash ALP are not susceptible to the caustic nature of any non supported system fluids Note All system fluid whether supported or non supported must be degassed p
33. 6 7 using 6 12 W waste container defined 3 37 3 43 replacing 3 44 wrist ground strap changing heads 2 11 changing tips 3 12 X X axis lubricating 1 12 Y Y axis lubricating 1 12 Biomek FX and FX P Laboratory Automation Workstations User s Manual
34. 8 Pod Refer to Section 3 8 Troubleshooting in Chapter 3 Span 8 Pod for more information Constant light curtain errors even when no violation Clean light curtain panels as described in Section 1 3 1 Light Curtain Maintenance Contact your Beckman Coulter Service Engineer Biomek FX and FX P Laboratory Automation Workstations User s Manual 1 14 Biomek FX Introduction A A Beckman Coulter Inc 1 4 1 Changing Fuses WARNING Do not remove tower covers to access electrical wiring or fuses Change only the fuses that are accessed from the outside of the instrument without removing covers Contact a Beckman Coulter Service Engineer if further access is required WARNING Turn off and unplug power to the instrument before changing fuses Failure to do so can cause electrical shock or equipment damage The Biomek FX instrument uses any AC power source between 100V and 240V The fuse holder holds two fuses Fuses are located in the outside right tower Figure 1 6 If power is lost check the fuses that are accessed from the outside of the instrument If fuses appear burned change them by completing the following 1 Turn off the instrument power switch 2 Unplug the instrument from the power source 3 Using a small flathead screwdriver pry open the fuse carrier containing two fuses Figure 33 4 from the AC power input module 40 pal 276 kPa MAX 100 pei 690 kPa O
35. Accuracy 00 0000 eee eee 5 27 Dia ItoUbleshvoumne 35 400 va cd baw pease toed Cae v eed i eee E a 5 28 6 Manually Controlling the Biomek FX in Biomek Software 6 1 OO th eas Been oe oO ane bh ew aetna ae 6 1 6 2 Accessing Manual Control 6 0chcceee ee cavees Chee deen Neen dnathades 6 2 6 3 Using Manual Control nsen ade tedvad oo dadueete cede us sedaen aces 6 3 6 3 1 Homing all Axes of the Pod or Pods 0 0 000 cece eee 6 3 6 3 2 Moving a Pod to a Specific Deck Position 000 6 5 6 3 3 Stopping a Pod si34 oats d howe ae eens ead eon Roatan es 6 5 6 3 4 Viewing the Firmware Version 0c cece cece een cence 6 6 6 3 5 Accessing Advanced Manual Control 00 0000 6 6 6 4 Using Advanced Manual Control with the Multichannel Pod 6 7 6 4 1 Viewing the Current Position of a Multichannel Pod 6 9 6 4 2 Moving a Multichannel Pod to a Safe Roving Height 6 9 6 4 3 Performing Relative Moves for the Multichannel Pod 6 10 6 4 4 Performing Absolute Moves for the Multichannel Pod 6 13 6 4 5 Extending and Retracting the Gripper 2 0000 6 14 6 5 Using Advanced Manual Control with the Span 8 Pod 6 15 6 5 1 Viewing the Current Position of a Span 8 Pod 6 17 6 5 2 Moving a Span 8 Pod to a Safe Roving Height 6 17 6 5 3 Performing Relative Moves for the S
36. Biomek FX in Biomek Software Chapter 6 Biomek FX and FX P Laboratory Automation Workstations User s Manual 1 2 Biomek FX Introduction The appendices include system specifications and instructions for integrating the Biomek FX with a Stacker Carousel e Appendix A Specifications e Appendix B Using a Stacker Carousel 1 1 1 Pod Configurations for the Biomek FX The Biomek FX instrument is capable of supporting up to two bridges Each bridge on the Biomek FX instrument holds one pod The available pod configurations for a single pod instrument are e One Multichannel Pod with either a 96 Channel 20 uL Head a 96 Channel 200 uL Head a 384 Channel Head or an HDR Tool Body e One Span 8 Pod Available pod configurations for a dual pod instrument are e Two Multichannel Pods any combination of heads e One Multichannel Pod any head and one Span 8 Pod Note For detailed information on the Multichannel Pod and interchangeable heads refer to Chapter 2 Multichannel Pod For detailed information on the Span 8 Pod and probes refer to Chapter 3 Span 8 Pod 1 1 2 Control Modes The Biomek FX is controlled using Biomek Software operated from the host computer The Biomek FX can be e Operated as a standalone instrument gripping and moving microplates without the assistance of a laboratory robot Integrated into an automated robotic system e Operated using a combination of these capabi
37. Choose Exit to close Manual Control To turn off the Speed Pump 1 Choose Instrument gt Manual Control Manual Control appears 2 Choose Advanced Controls 3 Select the desired Speed Pump Advanced Manual Control for the selected Speed Pump appears Figure 6 18 From Command choose Off Choose Go Choose Close to close Advanced Manual Control NY HD Nn A Choose Exit to close Manual Control Beckman Coulter Inc Appendix A Specifications Table A 1 System Specifications Item Description Environment Indoor use only Power Requirements US 100 120VAC 60Hz Europe 200 240VAC 50Hz Pneumatic Requirements Air Pressure 40 100 psi of compressed air 275 690 kPa of compressed air Air Flow 2 25 cfm 40 psi 0 064 m min 275 kPa System Fluid De ionized or distilled water Requirements Ambient operating temperature is 59 86 F 15 30 C System Fluid should be degassed for 24 hours prior to use Dimensions base unit with Canopy 152 cm L x 81 28 cm W x 139 7 cm H without Caonpy 152 cm L x 81 28 cm W x 109 22 cm H Weight 250 Ibs with one bridge one pod and a canopy 330 lbs with two bridges two pods and a canopy Ambient Operating 5 30 C 41 86 F Temperature Humidity Restrictions lt 85 non condensing 30 C 86 F Altitude Restrictions up to 2000m 6562ft Installation Category Category II Pollution Degree 2 Sound Pr
38. Deck Editor 2 Inthe Biomek Software open the deck that requires framing in the Deck Editor Verify that it reflects the current configuration of ALPs on the physical deck If it does not reflect the current physical deck configuration place devices on the appropriate deck locations in the Deck Editor by dragging and dropping from the list onto the deck When the Deck Editor reflects the configuration of the current physical deck proceed to step 3 Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 12 Framing the Biomek FX 3 Double click on the deck position containing the AccuFrame Position Properties appears Figure 5 9 Note The coordinates displayed are default values so the software must be taught precisely where the position is on the physical deck Name Ps ALP T ype OneB Three x cm Y cm Z cm Precision Pod1 Coordinates faa 434 fi 8 663 fa 5 7 Not Framed Pod2 Coordinates faa 434 fi 8 663 fi 5 7 Not Framed Pod Podi Advanced Mc Teach More gt gt C Pod2 Manual Teach Auto Teach Figure 5 9 Position Properties 4 Ifusing a dual pod system choose the pod being taught Pod 1 or Pod 2 5 Choose Auto Teach A Confirm similar to Figure 5 10 appears Note This moves the pod to the position being taught The framing probe should be above the AccuFrame in that position 2 The pod is about to go down 5 144 cm and teach position P9 Press
39. Enter the absolute coordinates to move to 9479 om zZ 4253 4 253 4253 4253 4253 4253 4253 4253 om vjs mop b b P hP bP pP P ul Span 8 84 mm These values will be converted to the necessary relative move numbers on the Advanced Manual Control form when you click OK Then you can press Go to move to these coordinates 7 Cancel Figure 6 14 Absolute Move for Span 8 Pod 5 Enter the desired X Y Z D and Span values Note Minimum and Maximum X Y Z and D values displayed in Hardware Setup indicate how far the pod is able to move These values are also displayed as a tool tip when hovering over the fields in Movement Vectors 6 Choose OK The Movement Vector changes to reflect the necessary relative move 7 Enter a value in Speed to specify the percent of the pod s maximum speed to use for the move 8 Choose Go The pod moves from its current position to a specified absolute position The new position is displayed in Current Position 9 Choose Close to close Advanced Manual Control 10 Choose Exit to close Manual Control ally Controlling the Biomek FX in Biomek Software 6 21 6 5 5 Setting Valve States The valves on the pumps may be set to allow system fluid to flow To and from the syringe with the supply container as the source destination of the fluid e To and from the syringe with tip as the source destination of the fluid e Through the valve without activating bypassin
40. FX P Laboratory Automation Workstations User s Manual xii Safety Information Pe Be AN A ZN A A AN A A A Beckman Coulter Inc CAUTION Do not use Correlate Pods in Hardware Setup without contacting a Beckman Coulter Service Engineer or Beckman Coulter Customer Technical Support CAUTION Do not use Correlate Z Axes in Hardware Setup without contacting a Beckman Coulter Service Engineer or Beckman Coulter Technical Support CAUTION Do not choose Cancel if testing is stopped before the probe sensitivities have been completed liquid level sensing will not function and Find Sensitivities must be repeated CAUTION Do not make any changes to the axes limits and pod settings without consulting a Beckman Coulter Service Engineer CAUTION Before selecting OK to home all axes the following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the gripper on the Multichannel Pod is retracted Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure that the two arms are not near each other at either end of the rail CAUTION Always ensure that the gripper is retracted before homing CAUTION When using Manual Teach to frame a position with the HDR Tool Body move the D axis all the way down to prevent the gripper from
41. Figure 32 3 The canopy stops light curtain violations resulting from movement above the instrument Biomek FX and FX P Laboratory Automation Workstations User s Manual 1 12 1 3 Biomek FX Introduction Preventive Maintenance A Beckman Coulter Inc To ensure optimum operation of the Biomek FX perform the following maintenance procedures as necessary WARNING To prevent injury use proper decontamination procedures e Wipe up all spills on the deck immediately e Approximately every six to twelve months schedule a Beckman Coulter service call to lubricate the linear rail 1 3 1 Light Curtain Maintenance To ensure optimum operation of the light curtain protective system perform the following maintenance procedures as necessary e Once a week verify proper operation of light curtain using Manual Control in the software refer to Chapter 6 Manually Controlling the Biomek FX in Biomek Software and the light curtain test rod supplied with the Biomek FX Insert the test rod no more than 1 past and approximately 21 above the front light curtain panel If side light curtains are installed on your Biomek FX instrument repeat this process approximately 24 above the side light curtain panel s Check to make sure the solid amber indicator light changes to blinking amber each time the light curtain is violated If not contact your Beckman Coulter Service Engineer e When necessary clean light curtain
42. Installing AccuFrame and frame the Multichannel Pod according to the procedure in Section 5 2 4 Framing the Position Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 8 Framing the Biomek FX 5 2 2 3 Attaching the Framing Shaft to the Span 8 Pod After homing all axes of the pod and installing and positioning the AccuFrame the framing shaft is attached to either probe 1 or probe 7 on the Span 8 Pod The framing shaft is attached to probe 1 when all positions except those along the front of the deck are framed Figure 5 6 Because probe 1 is unable to reach the AccuFrame when it is placed in the front deck positions probe 7 must be used to frame positions along the front of the deck Note Probes on the Span 8 Pod are numbered from back to front more specifically probe 1 is at the back of the Span 8 Pod and probe 8 is at the front of the pod Back of the Biomek FX Front of the instrument Biomek FX instrument The framing shaft is attached to probe 1 to frame all deck positions except those in the front row of the Biomek FX deck The framing shaft is attached to probe 7 to frame positions in the front row of the Biomek FX deck AccuFrame on ALP Figure 5 6 Framing a Span 8 Pod Beckman Coulter Inc Framing the Biomek FX To attach the framing shaft to a probe 5 9 1
43. Max 5 Choose Close to close Advanced Manual Control for the selected pod 6 Choose Exit to close Manual Control Biomek FX and FX P Laboratory Automation Workstations User s Manual 6 18 Manually Controlling 6 the Biomek FX in Biomek Sof 5 3 Performing Relative Moves for the Span 8 Pod Relative moves allow the pod to move from its current location to anywhere on the deck Relative moves are created in the Vector Builder using the Delta values or the vector can be manually edited Note Use Auto Clear when the values in Movement Vector must be reset to zero after the move is performed Turn off Auto Clear to retain the values after the move has occurred Choose Clear to set the values back to zero at any time To move a pod relative to its current position 1 2 3 4 5 Beckman Coulter Inc Choose Instrument gt Manual Control Manual Control appears Figure 6 2 Choose Advanced Controls Select Pod1 or Pod2 Advanced Manual Control for the selected Span 8 Pod appears Figure 6 12 Choose Clear to change the Movement Vector values to 0 Enter the desired X Y Z D and Span values in Movement Vector OR Set the Delta values as desired refer to Section 6 5 3 1 Setting Delta Values for the Span 8 Pod and click the appropriate buttons on Vector Builder refer to Section 6 5 3 2 Understanding and Using the Vector Builder for the Span 8 Pod until the desired values appear in Mov
44. OK to continue or Cancel to abort i Cancel Figure 5 10 Confirm 6 Visually verify that the framing probe is positioned to avoid hitting the wall of the AccuFrame and that it is positioned to lower within the AccuFrame tool Beckman Coulter Inc Framing the Biomek FX 5 13 7 Choose OK Note The pod lowers and moves around inside the AccuFrame automatically until it breaks both light beams Figure 5 1 The pod stops after teaching framing is completed and the two light beam indicators are illuminated Note An error message appears if both light beams are not broken when the pod is lowered into the AccuFrame If this occurs using Advanced Manual Control if necessary refer to Chapter 6 Manually Controlling the Biomek FX in Biomek Software move the pod until the probe breaks both light beams and all the indicator lights are on Choose Teach and the pod continues the teaching process The pod may also be moved by hand until the probe breaks both light beams and all the indicator lights are on 8 Wait until the pod stops moving and Teaching Instructions appears Figure 5 11 Teaching Instructions The location is 58 597 cm 18 751 cm 16 750 cm The change is 3 937 cm 0 088 cm 1 050 cm What would you like to do C Shift ALP Shift position cance Figure 5 11 Teaching Instructions 9 Choose from Shift deck Shift ALP or Shi
45. Pod To remove fixed tips 1 Unscrew the collar from the bottom of the probe by turning the collar counterclockwise Figure 3 10 lt __ Probe Tip Interface lt Collar lt _ Fixed Tip Figure 3 10 Fully installed fixed tip Beckman Coulter Inc Span 8 Pod 3 19 2 Pull the collar down and off the tip Figure 3 11 lt __ Probe Tip Interface lt Fixed Tip Collar Figure 3 11 Pulling the collar off the fixed tip 3 Gently pull the tubing and tip down from the Span 8 probe until enough tubing is extended to grasp between thumb and forefinger Figure 3 12 Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 20 Span 8 Pod 4 While holding the tip in one hand pull the tubing off the tip with the other hand Note Since the tubing is Teflon it may be difficult to hold Holding the tubing with a Kimwipe may prevent slipping while pulling the tubing off the tip Note Carefully twisting the fixed tip while pulling on the tubing may ease removal of the tip lt _ _ Probe Tip Interface lt Tubing lt _ Fixed Tip Figure 3 12 Removing a fixed tip 5 Repeat steps 1 through 4 to remove the remaining tips
46. Pod 3 53 3 6 3 Setting Address Switches When more than one Speed Pump is attached to the system a separate address must be set for each one to enable the software to identify the Speed Pump assigned a specific operation Addresses are set using the Address Switches on the back of the Speed Pump Figure 3 34 To set Speed Pump addresses 1 Make sure the power to the Biomek FX instrument is off 2 On the first Speed Pump make sure Address Switch 1 is set to 3 3 On the first Speed Pump make sure Address Switch 2 is set to 0 Note Default settings for all Speed Pumps are Address Switch 1 is set to 3 and Address Switch 2 is set to 0 Fora second Speed Pump make sure Address Switch 1 is set to 3 For a second Speed Pump turn Address Switch 2 to 1 Fora third Speed Pump make sure Address Switch 1 is set to 3 Fora third Speed Pump turn Address Switch 2 to 2 oN A Wn A Turn on power to the Biomek FX instrument The Speed Pump s can now be identified by the Biomek FX instrument Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 54 Span 8 Pod 3 Preventive Maintenance The Span 8 Pod requires little preventive maintenance however to ensure optimum operation perform the following maintenance procedures as necessary WARNING To prevent injury use proper decontamination procedures Z e Make sure all spills are wiped up immediately e Return fixed tips disposable tip mandrels
47. Uncoated fixed tips Barrier tips are recommended when using disposable tips with liquids that are susceptible to contamination such as blood Beckman Coulter Inc Span 8 Pod 3 11 3 3 3 1 Labware and Tip Compatibility with the Span 8 Pod The Span 8 Pod cannot access 1536 microplates However it can access all other types of labware supported by the Biomek FX instrument although specific types of tips are recommended to access specific types of labware Table 3 3 shows some tips supported by the Span 8 Pod and their accessibility to some types of labware supported by the Biomek FX instrument Note Table 3 3 is provided as a general reference and does not cover all commercially available microplate types It is the user s responsibility to verify that labware functions properly with the selected tip type For example a flat 384 square well microplate has a larger top cross sectional area than a flat 384 round well microplate therefore Fixed 100 tips can be used to access the flat 384 square well microplate but Fixed 60 tips would be more reliable when accessing the smaller area flat 384 round well microplate Consult Customer Technical Support for information on labware and tip compatibility not covered in Table 3 3 CAUTION Use labware and tip combinations labeled as Limited with AN caution Tips could become wedged inside labware picking the labware up with the probe when the probe is raised Tabl
48. a value in Speed to specify the percent of the pod s maximum speed to use for the move 8 Choose Go The pod moves from its current position to a specified absolute position The new position is displayed in Current Position 9 Choose Close to close Advanced Manual Control 10 Choose Exit to close Manual Control Biomek FX and FX P Laboratory Automation Workstations User s Manual Manually Controlling the Biomek FX in Biomek Sof 6 4 5 Extending and Retracting the Gripper Use Advanced Manual Control to extend or retract the gripper when changing the head on the pod CAUTION Select Move Z Max to move the pod to its highest point before extending the gripper To avoid breaking labware or bending the gripper fingers make sure the gripper will not hit any labware when extended CAUTION Do not retract gripper when it is holding labware A A Beckman Coulter Inc From Advanced Manual Control for the selected Multichannel Pod e Choose Extend Gripper to extend the gripper e Choose Retract Gripper to retract the gripper 6 5 Pod ally Controlling the Biomek FX in Biomek Software Use Advanced Manual Control for the Span 8 Pod to Note Viewing the Current Position of a Span 8 Pod Section 6 5 1 Moving a Span 8 Pod to a Safe Roving Height Section 6 5 2 Performing Relative Moves for the Span 8 Pod Section 6 5 3 Performing Absolute Moves for the Span 8 Pod Secti
49. after purging air and liquid to the Span 8 Tip Wash ALP Dispense Amount of liquid in milliliters that is dispensed to the reservoir of the Span 8 Tip Wash ALP Wash Amount of liquid in milliliters that is used to wash the tips of the Span 8 Tip Wash ALP in the eight cleaning wells Use speed pump When checked uses the speed pump to bypass the pump valves to accelerate the speed of the system fluid as it passes through the tubing and is dispensed from the tips into the Span 8 Tip Wash ALP Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 34 Configuring the Biomek FX in Hardware Setup 4 5 Saving Restoring and Deleting Settings The axes limits and pod settings may be saved restored and deleted using the Save Settings Restore Settings and Delete Settings options in Hardware Setup Note These options are particularly useful when changing heads on a Multichannel Pod Because a new D axis limit must be established when a head is changed these options allow the D axis limit to be saved and restored for specific head types When settings are no longer needed they may be deleted 4 5 1 Saving Pod Settings To save settings 1 In Hardware Setup select the appropriate Pod from the left pane to access the configuration area in the right pane Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 2 Choose Save Settings Save Configur
50. and extends the grippers Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 14 Multichannel Pod Beckman Coulter Inc 3 Turn off power and make sure the plastic bottom cover is removed Note Do not remove top cover 4 Position the strap portion of the wrist ground strap snugly around the wrist 5 Clamp the alligator clip of the wrist ground strap to the instrument deck base plate 6 With the chamfers Figure 2 9 pointed to the back of the instrument carefully slide the head up into the pod Coo Figure 2 9 Chamfer location on a Multichannel head Multichannel Pod 2 15 7 Slide the first shoulder screw Shoulder 1 into the head Figure 2 7 Make sure the shoulder screw is in far enough to engage the threads Figure 2 10 Sequence for installing four plunger screws and four shoulder screws on all multichannel heads 8 Using a 9 64 Allen wrench tighten the first shoulder screw until it is snug then back it off loosen it 1 2 turn Note This 1 2 turn allows the other three shoulder screws to be installed without skewing the head 9 Using the sequence designated in Figure 2 10 follow the procedure in step 6 to install the remaining three shoulder screws 10 After all four shoulder screws are installed go back and tighten them securely in the same order 1
51. axes 1 2 3 Pods and Heads Pods are self contained components supported and positioned by the bridges There are two types of pods available for the Biomek FX instrument e Multichannel Pod holds various removable and interchangeable heads such as the 96 Channel 200 uL Head that perform liquid handling operations and a pair of grippers for labware movement operations Refer to Chapter 2 Multichannel Pod for more information on the Multichannel Pod and interchangeable heads e Span 8 Pod holds a series of eight probes that perform liquid handling operations independent of each other Refer to Chapter 3 Span 8 Pod for more information on the Span 8 Pod The operation of the pods heads and probes are controlled by Biomek Software from the Biomek controller host PC Biomek FX and FX P Laboratory Automation Workstations User s Manual 1 8 Biomek FX Introduction 1 2 4 Deck The deck is the worksurface of the Biomek FX instrument The three section stainless steel deck is mounted on a cast aluminum base plate and laser etched with standard deck positions The deck also contains predrilled locating holes used to precisely position ALPs refer to Section 1 2 4 1 ALPs and thumbscrew holes to fasten ALPs securely to the deck Sections of the deck are removable so external devices refer to Section 1 2 4 2 Optional Devices and Solutions can be positioned within the Biomek FX perimeter making them accessible by a pod
52. be degassed prior to use Degassing the system fluid improves pipetting accuracy by reducing the amount of air in the system fluid The system fluid is compressed and released in the syringes to create a vacuum used during pipetting operations however air in the system fluid compresses at a different rate than the system fluid itself which can cause pipetting errors To reduce pipetting errors caused by air in the system fluid the system fluid must be degassed prior to priming the Biomek FX instrument with the system fluid Beckman Coulter Inc Span 8 Pod 3 47 Degassing the system fluid is accomplished by letting the system fluid rest in the supply container for 24 to 48 hours prior to attaching it to the Biomek FX instrument refer to Section 3 5 3 Supply Container The lack of motion allows the air bubbles in the system fluid to escape or burst prior to use Note It is recommended that extra supply containers be degassed at all times so that properly degassed system fluid is readily available when a supply container must be replaced refer to Section 3 5 3 Supply Container 3 5 5 2 Priming the System with System Fluid Priming the system is the initial aspiration of system fluid into the tubing for the Span 8 Pod It ensures that system fluid is in all the tubing prior to purging the system of air and performing any pipetting actions Priming the system with system fluid is accomplished through Manual Control refer to Secti
53. be homed each time the Biomek FX instrument is powered on Depending on the type of pods on the system a Warning Figure 4 2 appears After confirming that the actions have been addressed properly choose OK Refer to Section 6 3 1 Homing all Axes of the Pod or Pods for more information on homing all axes of the pods Add Device Installs a device Remove Device Removes a device Accept Saves all changes to the instrument and closes Hardware Setup Choose this option after the device has been installed and configured Cancel Closes Hardware Setup without saving the modifications to the instrument ee a x Make sure there is no liquid present in the tips CAUTION Before selecting OK to home all axes the following conditions must be met e Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the grippers on the Multichannel Pod are retracted Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure that the two arms are not near each other at either end of the rail Cancel Figure 4 2 Warning after choosing Home All Axes Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 4 Configuring the Biomek FX in Hardware Setup 4 2 Configuring the Biomek FX Instrument The Biomek FX instrument is configured in Hardware
54. being completed Ensure there is system fluid in the supply container refer to Section 3 5 3 Supply Container Aspirate and dispense actions are inaccurate Ensure tubing connections are secure refer to Section 3 3 5 Fixed Tips and refer to Section 3 3 6 2 Removing the Disposable Tips Option Ensure fixed tip seating is secure refer to Section 3 3 5 Fixed Tips Ensure disposable tip mandrels are correctly mounted refer to Section 3 3 6 1 Installing Disposable Tip Mandrels Ensure system fluid and tubing have been purged of air refer to Section 3 5 1 System Fluid Tips are clipping edges of labware or not able to access labware Reframe the position Check to see if tip or mandrel is obviously bent Contact a Beckman Coulter Service Engineer Speed Pump is not operational Check the CAN cable connection Span 8 Pod 3 57 Table 3 8 Span 8 Pod Troubleshooting Continued If The Span 8 Pod has not performed aspirate or dispense actions since installation of the Speed Pump Then Make sure valves are set to bypass in Advanced Manual Control refer to Section 6 5 5 Setting Valve States Contact a Beckman Coulter Service Engineer The Speed Pumps CAN connection has been verified its valves are set to bypass and the Speed Pump is still not operational Check to make sure supply container is full The Speed Pumps CAN connection has been verified
55. detected that were not configured in Hardware Setup or if there are probes that must be calibrated Find Sensitivities Tip Configuration appears Figure 4 19 Refer to Section 4 4 1 Configuring the Probes for Fixed or Disposable Tips to correctly configure the probes in Hardware Setup If probes are not calibrated configure the disposable tip in Find Sensitivities Tip Calibration to match the tip that is currently attached to the probe Figure 4 19 Choose Next to proceed through the wizard Find Sensitivities Start Load AP96_200uL_LLS 7 tips onto mandrels W A E HH at Position P6 and unload them v once testing is completed Click on a deck position to select the tip load position AA Tubes2 Figure 4 18 Find Sensitivities Load Tips Load Tips Choose Position Test itivities Update Settings Configure the disposable tips to match the tips that are currently attached to the probes Itis important that the described configuration matches the physical Tip Configuration configuration otherwise collisions may occur damaging the device Load Tips Please note that if you need to switch from a disposable tip to a fixed tip you must exit this wizard and perform that modification in Hardware Setup Probe 1 Probe 2 lt None gt x Probe 3 lt None gt pa lt None gt Probe 4 lt None gt bd Probe 5 P200_LLS g Probe 6 P200_LLS Probe 7 P200_LLS Probe 8 P2
56. direction and cag E Ten Nara Df E F 5 E E E E amount the Pawn Checkmarks select probes that pod and or Xm Yim _ Span mm descend when Go is selected pr obes move X cm Y cm Span mm Pod jo fp o These Pod fo jo f vi v2 v3 v 4 v5 v6 v v8 choices 2 em fo speed ioo e M uszMeve 2m fo fo fo fo fo jo jo fo change the ol fi D ul fo fo fo jo fo jo fo jo values in the Delta LLS Z Move Bo O Movement Vector Movement Controls the amount of Verifies 5 Indicates the amount the Vector change applied to the liquid level pod and or probes move Movement Vector when sensing when Go is selected the Vector Builder is used Figure 6 12 Overview Advanced Manual Control for a Span 8 Note See Table 6 2 for further descriptions Biomek FX and FX P Laboratory Automation Workstations User s Manual 6 16 Manually Controlling the Biomek FX in Biomek Sof Beckman Coulter Inc Refer to specific subsections for instructional use Table 6 2 Advanced Manual Control selection areas for the Span 8 Pod Area Absolute Move Description Builds a vector to an absolute coordinate from the current coordinate Active Axes Checkmarks disable any probes that should not descend when Go is clicked Auto Clear When checked each time Go is selected the Movement Vector resets to the 0 vector no movement Note Auto Clear is on by default Clear Sets movement vector en
57. eight probes can expand and collapse Note The span between each of the eight probes is always equal 3 2 2 Control Modes Commands entered via the Biomek Software on the host computer control the operations of the Span 8 Pod Manual control associated with the operation of the Span 8 Pod is accessed through the Biomek Software Refer to Chapter 6 Manually Controlling the Biomek FX in Biomek Software for information on manually controlling the Span 8 Pod Beckman Coulter Inc Span 8 Pod 3 5 3 3 Interchangeable Tips CAUTION Use Manual Control to move the probes in the Y axis UN Dragging the probes by hand may affect alignment and the ability to access labware WARNING Use care when handling septa fluted tips they are extremely sharp CAUTION Use care when handling septa fluted tips they are extremely fragile Interchangeable tips are attached to the Span 8 probes to perform liquid handling procedures The following interchangeable tips are used on the Span 8 Pod although septa fluted are available only with a Biomek FX instrument Fixed e Disposable e Septa Fluted fixed but also capable of piercing septum when the Requires Piercing Tips property of the Septum Piercing Tube Racks is checked in the Labware Type Editor refer to the Biomek Software User s Manual Chapter 8 3 6 Editing Labware Type Properties Septa fluted tips are available only with a Biomek FX instrument
58. get iea ROK AH DE ERY HARARE E UA RRR KES 3 1 3 1 1 Main Components of the Span 8 Pod 0 00 cee eee eee 3 3 PrObES a acetone Soar e E aeons gape ah ming aca a are wee pean EEEE are 3 4 3 2 1 Probe MoyeMmentS oa buen ohare nren r E ee aces eae GRA REESE 3 4 S22 Control Mod s we eden aes eae n year wed a EE E daa 3 4 Interchangeable Tips s ncicscd ace d420kt deka Ae eR ERR aS EER MEER EES 3 5 3 3 1 Liquid Level Sensing 4 6 4 0 45 044 seen annann 3 6 3 3 2 Clot Detection available only with a Biomek FX Instrument 3 7 3 3 3 Selecting Which Type of Tips to Use 0 0 0 0 0 0c cece eee 3 9 3 3 4 Electro Static Discharge Protection when Changing Tips on the Span 8 Pod 3 12 335 Fix d ViPS 155 4954 de Oy oa oe Ba oh a ee es das 3 13 3 3 6 Disposable Tip Mandrels 0 0 0 c eee eens 3 20 Beckman Coulter Inc WF 10 Woy m Oxo aic y ay xvii 3 4 Puiip Assembly iiscta cea soda hohe ea dk Ra siit eri toads kin riras 3 28 3 4 1 PUMPS 4 00254 iird ood shadebwio ed daa otaa cakho tak mead eed eae 3 29 3 4 2 I ceases sta ee een sag a a Pa we Te a eee 3 29 S28 MMi Syste 2056 ieoenyd er Esran E odes Chee PARES eead ieee ees 3 37 3 5 1 System Fluid are pipe retrad ata ae rean a E EEE EE ec aE 3 37 3 5 2 TUDIN eeir dk cts Moana Rank pe EE EER EREE EVRIKA 3 39 3 5 3 Supply Container sesceidsn regii eaten sae RHEE Co e E 3 40 3 5 4 Waste Container cacvasscsadcads dtdadtahd baeee de dsahiesaaed 3 43 3 5 5 P
59. ground strap to the instrument deck base plate Figure 2 3 6 Using a Phillips screwdriver remove the three screws from the plastic bottom cover Figure 2 4 Figure 2 6 Bottom cover removed from the pod 7 Carefully open and remove the bottom cover Do NOT removethe top cover 2 3 2 1 2 Removing the Head To remove the head from the pod Note Remove the pin plate before removing an HDR Tool Body refer to Section 2 3 1 2 Removing Pin Plates 1 Make sure the head is completely in the down position D axis before proceeding with removing the head This helps to ensure that the seals are protected during removal 2 Using the 9 64 Allen wrench provided remove the four plunger screws from the plunger plate Figure 2 5 in the sequence designated in Figure 2 6 Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 12 Multichannel Pod CAUTION Before removing the fourth shoulder screw take hold of the UN head firmly to make sure it does not fall once all screws are removed 3 With the same 9 64 Allen wrench remove the four shoulder screws from the mandrel plate Figure 2 7 in the sequence designated in Figure 2 8 TTL Bt ff SPA Ta Ia Lin Pa SS Plunger plate Mandrel plate apa Plunger i screws 2 Shoulder g g screws li li Figure 2 7 Plunger screws and shoulder screws rem
60. i CAUTION Do not make any changes to the pod axes limits in Hardware Beckman Coulter Customer Technical Support Hardware Setup is used for Configuring the Biomek FX Instrument Section 4 2 e Configuring a Multichannel Pod Section 4 3 e Configuring a Span 8 Pod Section 4 4 e Saving Restoring and Deleting Settings Section 4 5 Note Any active ALPs that require a CAN communication or other devices integrated on the deck of the Biomek FX must also be configured in Hardware Setup Refer to the ALPs User s Manual or the specific device integration manual for instructions on configuring the ALP or device in Hardware Setup Note Refer to Section B 4 Configuring the Stacker Carousel in Hardware Setup for information on configuring the Stacker Carousel in Hardware Setup Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 2 Configuring the Biomek FX in Hardware Setup 4 1 1 Accessing Hardware Setup Hardware Setup Figure 4 1 is accessed from within Biomek Software To access Hardware Setup 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software Biomek Software appears 2 From the Instrument menu choose Hardware Setup Hardware Setup appears Figure 4 1 Biomek Hardware Setup Reconnect Hf Home all Axes db Add Device s Remove Device Y Accept X lt Cancel Biomek None Serial Number fj AccuFrame 96 Podi Port Simulate 7
61. in Hardware Setup 3 Choose Next Find Clot Detection Sensitivities Load Tips appears Beckman Coulter Inc Figure 4 27 Note If tips that were not configured in Hardware Setup are detected or if there are probes that must be calibrated Find Clot Detection Sensitivities Tip Configuration appears If probes are not calibrated configure the disposable tip in Find Clot Detection Sensitivities Tip Calibration to match the tip that is currently attached to the probe Choose Next to proceed through the wizard Find Clot Detection Sens Start Load AP96_200uL_LLS v tips onto mandrels BH at Position P3 and v once testing is completed Click on a deck position to select the tip load position Load Tips Cho sition Test Sensitivities Update Settings TRI Ps wee P10 P14 Pil PiS Figure 4 27 Find Clot Detection Sensitivities Load Tips Note Ifall probes are configured with fixed tips Find Clot Detection Sensitivities Choose Position appears rather than Find Clot Detection Sensitivities Load Tips Go immediately to step 9 In Load choose the appropriate tip type Note Any probe configured to use disposable tips must be loaded with a conductive tip In mandrels choose the desired probes Click on the position where tips should be loaded Choose unload them to unload tips when Find Clot Detection Sensitivities is completed OR Choose leave them on to leave tips on when Find Clot
62. in the D axis and span from 9mm to 20mm between the probes in the S axis Figure 3 1 The pipetting action of the Span 8 Pod is accomplished using pumps and either fixed or disposable tips with or without liquid level sensing capabilities The Span 8 Pod contains its own electrical cabling and communication with the base unit and it interacts with ALPs located over the entire deck of the Biomek FX Note When installed on a dual pod system the pod mounted on the left side of the Biomek FX instrument is capable of accessing all ALPs on the deck excluding those in the far right column while a pod mounted on the right side of the Biomek FX instrument is capable of accessing all ALPs on the deck excluding those in the far left column The sections in this chapter include e Probes Section 3 2 Interchangeable Tips Section 3 3 e Pump Assembly Section 3 4 e Liquid System Section 3 5 e Speed Pump Section 3 6 Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 2 Span 8 Pod Preventive Maintenance Section 3 7 Troubleshooting Section 3 8 System fluid moves through the tubing Span 8 Pod rm Pa o wih a l NP 0 ae gt Pumps and syringes Beckman Coulter Inc Span 8 Pod 3 3 3 1 1 Main Components of the Span 8 Pod The Span 8 Pod houses the operating mechanisms communi
63. mounting holes for s y back mount Upper alignment bracket Horizontal alignment bracket Figure B 6 Attach alignment bracket Using a Stacker Carousel B 7 7 Place the riser plate over the alignment pins of the horizontal alignment bracket Figure B 7 8 Verify that the riser plate is set firmly on the Biomek FX alignment bracket and the table Riser plate Riser alignment pins Figure B 7 Attaching riser plate Biomek FX and FX P Laboratory Automation Workstations User s Manual B 8 Using a Stacker Carousel B 2 2 2 Attaching the Stacker Carousel Attach the stacker as follows 1 Place the stacker onto the riser plate over the riser alignment pins Figure B 8 Figure B 8 Stacker attachment 2 Level the stacker 3 Adjust the height of the stacker until the distance between the Biomek FX deck surface and the top of the flat surface of the stacker shuttle measures 5 in 1 8 in Note Ifnecessary refer to the Stacker Carousel User s Manual for instructions on how to adjust the height of the stacker B 2 3 Attaching the Side Shield Attach the side shield as follows 1 Attach the cutout blank and Plexiglas side shield in the appropriate orientation for the shuttle to access the cutout without obstruction Figure B 9 through Figure B 12 Note Use the same cutout blank to form either the rear or front cutout orientations by t
64. narrow side scanning position Beckman Coulter Inc Using a Stacker Carousel B 21 Laser beam Figure B 27 Bar code reader in front narrow side scanning position bottom view Bar code reader Bar code reader laser beam Fastener collars Bracket fasteners Bracket Figure B 28 Bar code reader attachment bracket Biomek FX and FX P Laboratory Automation Workstations User s Manual B 22 Using a Stacker Carousel B 3 4 Applying Power to the Bar Code Reader CAUTION To avoid serious damage to the instrument make sure that AN the laboratory site voltage frequency matches the voltage frequency that was ordered for the instrument WARNING Do not attempt to remove or replace covers while the unit is powered on Disconnect power before removing or replacing a cover Apply power to the bar code reader as follows 1 Secure the MS710 power cable to the rear panel of the Stacker Carousel Note Make sure the cable is connected to the port marked BCR and the cable routing does not interfere with the operation of the Stacker Carousel 2 Turn on the Stacker Carousel power switch B 3 5 Aligning the Laser Beam WARNING Avoid direct exposure to the laser beam Never look directly into the laser beam and never leave the laser on open or unattended WARNING Always have the laser module access cover located on the bar code read
65. of the pump assembly provide a vacuum which allows the aspirate and dispense actions of the Span 8 Pod E A Pump Assembly Pump Syringe The pump assembly consists of the eight pumps one for each of the Span 8 probes Figure 3 21 Span 8 pump assembly Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 30 Span 8 Pod 3 4 1 Pumps The pumps control the flow of system fluid into the syringes tubes and probes of the Span 8 Pod The amount and direction for the flow of system fluid is controlled by setting the state of the valve in the pump The valve can be set to one of three states Input Opens the valve and allows system fluid to flow to and from the syringe with the supply container as the source destination of the fluid e Output Opens the valve and allows system fluid to flow to and from the syringe with the tip as the source destination of the fluid e Bypass Opens the valve and allows system fluid to flow through the valve without activating bypassing the syringe Note The state of a valve can be changed in Advanced Manual Control if necessary refer to Section 6 5 5 Setting Valve States Input Output Valve lt Pump Syringe thumbscrew Figure 3 22 Pump and syringe 3 4 2 Syringes A syringe is housed in each of
66. panels with a lint free cloth e Once every 2 3 months clean light curtain panels with a non abrasive cleaner making sure not to scratch the strip Biomek FX Introduction 1 13 1 4 Troubleshooting CAUTION If service is required contact a Beckman Coulter Service UN Engineer Perform the troubleshooting techniques provided in Table 1 2 when necessary In the case of any other instrument related problems contact a Beckman Coulter Service Engineer Note For troubleshooting information regarding the specific pods refer to the Troubleshooting section in those respective chapters Table 1 2 Troubleshooting the Biomek FX Instrument If Then All indicator lights are out Check fuses The power is on but system does not Check fuses run The fuses appear burned or filaments Change fuses refer to Section 1 4 1 are broken Changing Fuses All indicator lights are out the power Contact a Beckman Coulter Service is on the fuses are okay and the Engineer system does not run Contact a Beckman Coulter Service Engineer Power is lost to pod Contact a Beckman Coulter Service Engineer Y axis motion is choppy A grinding or growling noise is heard Contact a Beckman Coulter Service Engineer Experiencing problems relating to the Refer to Section 2 6 Troubleshooting Multichannel Pod in Chapter 2 Multichannel Pod for more information Experiencing problems relating to the Span
67. position to frame Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 18 Framing the Biomek FX 9 Choose Next Teach X Y appears Figure 5 17 Delta value The magnitude of change applied to the tips Manual Teaching each time a directional button is selected The tips should be about a half centimeter above the plate Drag the circle to where the tips are in relation to the Well Commer and press Go Press Next gt when the tips are directly above their Warmin target Warning Delta 0 05 fom a Setup A F s Left 4m Bon 4 TeachX Y gt 4 Fwd Down m Hysteresis compensation Total Moved From Start cm TeachZ o o o Reset Well Comer well Center Go Graphic alignment tool The graphic alignment tool is a visual representation of Cancel the tip small circle and the wells of the microplate P Directional buttons the four squares The small circle is moved until it The directional buttons move the represents the tip s current physical location in relation pod by the amount shown in Delta to the wells of the microplate on the ALP with each press of a button Hysteresis compensation Leave Hysteresis compensation at the default setting of On This compensates for any variance along the X axis from the front of the bridge to the back of the bridge The Biomek FX determines a pods po
68. probe type click on the desired probes to configure from the graphical representation of the probes Note The graphical representation of the probes changes according to the type disposable or fixed Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 14 Configuring the Biomek FX in Hardware Setup 3 From the drop down menu choose the tips in Probes 1 4 use tips Note To choose probes 1 through 8 at one time hold down Ctrl with probes 1 through 4 highlighted and then click on probes 5 though 8 Note Probes 1 through 4 may be configured with one type of fixed tips or disposable tips and probes 5 through 8 may be configured with another type of fixed tips or disposable tips To mix the type of tips select probes through 4 and choose the tip type Then select probes 5 through 8 and choose the tip type Note When tips are changed from disposable to fixed or from fixed to disposable a Warning Figure 4 9 appears on Hardware Setup that the pod must be homed and Find Sensitivities performed When Accept is chosen another device is configured in Hardware Setup or a dialog is chosen from Tools another Warning Figure 4 10 appears that the pod must be homed and Find Sensitivities performed Biomek Hardware Setup b Reconnect At Home All Axes G3 Add Device S Remove Device lt J accept gt lt Cancel Biomek FX SN None fj AccuFrame 96 Podi 8 Pod2 amp Devices
69. reader to the Stacker Carousel shuttle as follows 1 Remove the mount access cover Figure B 15 and store in a safe place Bar code reader shuttle mount access Shuttle mount access cover Figure B 15 Bar code reader shuttle mount access cover removed 2 Screw mount standoffs onto shuttle arm assembly mount points inside shuttle mount access Figure B 16 3 Using the fasteners attach mount base to standoffs Mount standoffs Fasteners Mount base Figure B 16 Mounting bracket attachment Beckman Coulter Inc Using a Stacker Carousel B 15 4 Attach bar code reader bracket assembly to bar code reader mount base Figure B 17 Bar code reader laser beam Bar code reader mount base Bar code reader bracket assembly Figure B 17 Mounting bar code reader Biomek FX and FX P Laboratory Automation Workstations User s Manual B 16 Using a Stacker Carousel B 3 3 Positioning Bar Code Reader The Stacker Carousel bar code reader can be mounted to scan any one of the four sides of a microplate Note For maximum scanning reliability it is recommended that labels be applied to the narrow sides of the microplate This label orientation accommodates scanning of skewed labels and requires less adjustment of the bar code reader position Position the bracket so the highest edge is parallel with and away f
70. shuck tube up over the disposable tip mandrel to the tip interface Figure 3 15 Probe Tip Interface Disposable Tip Mandrel lt Tip Shuck Tube Figure 3 15 Installing a tip shuck tube on a disposable tip mandrel Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 24 Span 8 Pod CAUTION Do not overtighten the tip shuck tube Problems with A N unloading tips may result 7 Screw the tip shuck tube clockwise into the tip interface until a tight fit is achieved Make sure the tip shuck tube is securely tightened to prevent it from working off during operation The disposable tip mandrel is now installed Figure 3 16 Probe Tip Interface Tip Shuck Tube Disposable Tip Mandrel Figure 3 16 Installed disposable tip mandrel 8 Repeat steps 1 through 7 for any other probes requiring disposable tips Beckman Coulter Inc Span 8 Pod 3 25 3 3 6 2 Removing the Disposable Tips Option To remove the disposable tips option the disposable tip mandrels must be removed from the Span 8 probes WARNING SPILL HAZARD WARNING Use an appropriately contained environment when using hazardous materials WARNING Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety o
71. syringes speed pumps and accessories to their original packaging when they are not in use e Check for leaks at all tubing fittings periodically to make sure that all are tight Note When tubing is repeatedly removed and reattached the end of the tubing may stretch out or split If tubing is not forming a tight fit cut off approximately 1 2 of tubing to remove the damaged portion before attaching the tubing to the port e Check that disposable tip collars are securely tightened to the tip interfaces each week Beckman Coulter Inc Span 8 Pod 3 55 3 8 Troubleshooting WARNING Do not connect or disconnect any cable while power is UN applied to the Biomek FX Perform the troubleshooting techniques presented in see Table 3 8 when necessary Table 3 8 Span 8 Pod Troubleshooting If Then Power is lost to the pod Contact a Beckman Coulter Service Engineer Motion is lost in an axis Contact a Beckman Coulter Service Engineer The probes do not work properly Contact a Beckman Coulter Service Engineer The Span 8 Pod is leaking from Cut off approximately 1 2 of tubing to tubing connections remove the damaged portion before reattaching the tubing Leaking is occurring around syringes Tighten the syringes Leaks are occurring around fixed tips Make sure the tip is inserted securely into the tubing Cut off approximately 2 of tubing off the end to ensure a tight fit Make sure the col
72. the Stacker Carousel User s Manual Beckman Coulter PN 148598 A bar code reader may be mounted to the Stacker Carousel to scan any one of the four sides of a microplate Note For maximum scanning reliability it is recommended that labels be applied to the narrow sides of the microplate This label orientation accommodates scanning of skewed labels and requires less adjustment of the bar code reader position The sections in this appendix include Integrating the Stacker Carousel Section B 2 Integrating the Bar Code Reader Section B 3 e Configuring the Stacker Carousel in Hardware Setup Section B 4 Framing the Stacker Carousel Section B 5 Biomek FX and FX P Laboratory Automation Workstations User s Manual B 2 Using a Stacker Carousel B 2 Integrating the Stacker Carousel Note Install only in conjunction with the Biomek FX model incorporating Plexiglas safety shields along the sides of the instrument in lieu of side light curtains Figure B 1 Stacker Carousel Integrating the Stacker Carousel into the system requires e Positioning the Stacker Carousel on the Deck Section B 2 1 e Mounting the Stacker Carousel Section B 2 2 e Attaching the Side Shield Section B 2 3 e Operating the Stacker Carousel Section B 2 4 Beckman Coulter Inc Using a Stacker Carousel B 3 B 2 1 Positioning the Stacker Carousel on the Deck The Stacker Carous
73. the X or Y axis Last Validation Set by a Beckman Coulter Service Engineer Maximum X Y Z D and Span S The maximum position the pod may go to along the X Y Z D and Span S axes relative to the Home position Set by using the appropriate buttons under the X cm and Y cm columns Do not change the axes limits without contacting a Beckman Coulter Service Engineer Minimum X Y Z D and Span S The minimum position the pod may go to along the X Y D and Span S axes relative to the Home position Set by using the appropriate buttons under the X cm and Y cm columns Do not change the axes limits without contacting a Beckman Coulter Service Engineer Post Run Wash Volume Amount of liquid in mL run through the probes after a method is run Probe Size Thickness of the probe along the Y axis Speed Limit Controls the speed for pod movement based on a percentage of its maximum speed System Trailing Airgap Volume of air between the system liquid and the additional air gaps that are drawn into the tip Unload Speed Controls the pod speed when unloading tips based on a percentage of its maximum pod speed Recommended that this be modified only by a Beckman Coulter Service Engineer Note Pod properties for a Span 8 Pod may be saved restored and deleted using Save Settings Restore Settings and Delete Settings refer to Section 4 5 Saving
74. the pod with 0 percent meaning use current speed Vector Builder Relative moves which allow the pod to move from its current location to anywhere on the deck are created using the Vector Builder Each time a Vector Builder button is pressed the Movement Vector is changed in the corresponding axis by the amount indicated in Delta Up and Down move the pod in the Z axis Left and Right move the pod in the X axis and Back and Fwd move the pod in the Y axis Squeeze Aspirate and UnSqueeze Dispense move the head in the D axis based upon the selection made in D Units Beckman Coulter Inc ally Controlling the Biomek FX in Biomek Software 6 9 6 4 1 Viewing the Current Position of a Multichannel Pod The current position of a Multichannel Pod is displayed as four coordinates Each axis coordinate is the distance from the home position X Y and Z are displayed in centimeters The D axis is displayed in either centimeters cm or microliters uL Refer to the description for D Units in Table 6 1 To view the current position of a pod 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Multichannel Pod appears Figure 6 9 The current position is shown in Current Position 4 Choose Close to close Advanced Manual Control 5 Choose Exit to close Manual Control 6 4 2 Moving a Multi
75. tubing from the Biomek FX instrument to the vacant port on the tubing connector Note A Speed Pump must be configured in Hardware Setup after installation to the Biomek FX instrument refer to Section 4 4 2 Configuring a Speed Pump CAUTION Do not remove the cover of the Speed Pump WARNING Turn off power to the Biomek FX before connecting CAN communication cables A A Beckman Coulter Inc To install a Speed Pump 1 Turn off power to the Biomek FX instrument before attaching the Speed Pump 2 Using the Speed Pumps CAN communication cable connect the Speed Pump to a CAN communications port on a Biomek FX tower Note Three CAN communications ports are located on each side of the Biomek FX instrument When making the decision on which CAN communications port to use consider the number of CAN communication ports in use and try to even the load Note Up to three Speed Pumps can be chained together Up to two Speed Pump can connect toa CAN communications port on another Speed Pump but one of the three Speed Pumps must be connected to a CAN communications port on a Biomek FX tower Span 8 Pod 3 51 3 Position the Speed Pump in an off deck location that does not interfere with the normal operation of the Biomek FX instrument Note The Speed Pump must be in line between the supply container the source of the system fluid and the pumps 4 Remove the ferrule from the vacant port on the tubing connector Fig
76. view tora Stacker Carouse loran e A S B 27 Figure B 35 Adding the Accuframe and Framing Tool Adaptor to the Stacker Carousel shuttle via isssindsiascccnessiasianesd pica sbansconceUerisntepnvasacnseaacausnass B 30 Biomek FX and FX P Laboratory Automation Workstations User s Manual List of Tables Table l 1 Indicator Light KEY acascssedassasspuceseddasncessnspiddvsnsnncwersaadacanschesesbdnstnccusis aE E AeA REEE 1 6 Table 1 2 Troubleshooting the Biomek FX Instrument s sssesseseesesesssesesessrsrtsessesresssseres 1 13 Table 2 1 Multichannel Pod Axes Movement ce eeseecsssscesecsseceeesecaeeeneeeeeeseeeaecsaecaeeeaeesneees 2 4 Table 2 2 Tip compatibility and max volumes on Multichannel Heads ce eeeeeceeeeeeteeseeees 2 5 Table P ig Pin Ej tcc ites tu eae veencs ede arsenate cana a mea ee ceca aa eee corneas 2 6 Table 2 4 Multichannel Pod Troubleshooting ccceeceesceesseesseceseeeeeeeeseeceueceseeseeeenseeesaeenes 2 21 Table 3 1 Span 8 Pod Axes Movement ssicscxdvnsascsuaniosssravndesseccnsdacseesnesseieansdlund aiaiai 3 4 Table 3 2 Span 8 Tip Types prescient ersin aee EEEE EEEE S EEEE aAa 3 9 Table 3 3 Labware and Tip Compatibility sccucccc cus scesasbetsateasvcacieenistaaxavedeedcannnciavuinectouieacdes 3 10 Table 3 4 Span 8 Cutoff Velocities for Syringes s ss ssesessssessesessressessrssressesseesesseeseeseessre 3 36 Table 3 5 Parts and materials along the Span 8 fluid flow path
77. with this instrument Disposable 20uL LLS AP96_20 LLS Yes maximum of 100 uL liquid capacity up to 120 Disposable 20uL Span_8 20uL No uL air capacity depending on syringe size Disposable 200uL LLS AP96_200_LLS Yes maximum of 220 uL liquid capacity up to 240 Disposable 200uL Span_8 200uL No HL air capacity depending on syringe size Disposable 20uL Span_8 20uL_Barrier Yes maximum of 20 uL liquid barrier tips and or air capacity depending on syringe size Disposable 200 uL Span_8 200uL_Barrier Yes maximum of 125 uL barrier tips liquid and or air capacity depending on syringe size Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 10 Span 8 Pod Table 3 2 Span 8 Tip Types Tip Type Tip Name LLS CD Tip Volume Software Capable Disposable P1000 uL Span8_P1000_LLS Yes maximum of 1000 uL sterile and non sterile liquid and or air capacity depending on syringe size Disposable P1000 uL Span8_P1000_LLS Barrier Yes maximum of 1000 uL barrier tips liquid and or air capacity depending on syringe size Coated refers to tips covered with a Teflon coating The coating assists in preventing cross contamination Solutions are less likely to adhere to the Teflon coating than to uncoated stainless steel fixed tips and are easier to wash off thereby reducing the potential for cross contamination Note The Biomek Software does not distinguish between Coated
78. 00 pL Head 7 Last Validation Not Specified Set Validation Time B Podz Axis Limit Settings je A co Ken aE D ul D cm _Comelte Pod Sods r ShakerALPO Minimum 63 fag 15 5 58 434 fo614 A Serie e725 j2 0s f22 zz aa eZ eF SpeedPump1 SetX SetY SetZ SetD Change Head oF StirrerALPO g7 StirrerALP1 Y Additional Pod Settings g7 TiploaderO g7 Tiploader1 amp Digital Devices E Simulator E Camera e Materials Stacker Carousels Tip And Gripper Settings BiomekFX Figure 4 4 Hardware Setup showing the configuration view for a Multichannel Pod 4 Choose Home All Axes The following Warning appears Figure 4 5 Note Choosing Home All Axes homes all of the axes for all pods Warning xl CAUTION Before selecting OK to home all axes the Following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the grippers on the Multichannel Pod are retracted Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure that the two arms are not near each other at either end of the rail Cancel Figure 4 5 Warnings must be addressed before homing process begins 5 Choose OK after confirming that the Warning has been addressed appropriately
79. 00_LLS Me Figure 4 19 Find Sensitivities Tip Configuration Note Ifall probes are configured with fixed tips Find Sensitivities Choose Position appears rather than Find Sensitivities Load Tips Go immediately to step 9 Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 24 Configuring the Biomek FX in Hardware Setup 4 In Load choose the appropriate tip type Note Any probe configured to use disposable tips must be loaded with a conductive tip 5 In mandrels choose the desired probes 6 Click on the position where tips should be loaded 7 Choose unload them to unload tips when Find Sensitivities is completed OR leave them on to leave tips on when Find Sensitivities is completed 8 Choose Next Tips are loaded Find Sensitivities Choose Position appears Figure 4 21 Note Ifany tips fail to load follow the recovery instructions displayed on Find Sensitivities Load Tips Figure 4 20 Find Sensitivities 5j Start Load 4P96_200uL_LLS 7 tips onto mandrels ll A E HH NM Load Tips at Position P6 and unload them x once testing is completed Probes 1 2 3 4 5 6 7 and 8 were not properly loaded with conductive tips Pressing Next will attempt to reload tips starting with the next available tip Cho anion Do not remove any tips that were loaded properly Test icing Click on a deck position to select the tip load position mit 7 Update Settings Rl
80. 1 Removing the Bottom Cover Note Do not remove top cover 5 Position the strap portion of the wrist ground strap snugly around the wrist 6 Clamp the alligator clip of the wrist ground strap to the instrument deck base plate 7 With the chamfers Figure 2 11 pointed to the back of the instrument carefully slide the HDR Tool Body up into the pod 3 Figure 2 11 Chamfer locations on the HDR Tool Body Beckman Coulter Inc Multichannel Pod 2 17 8 Slide the first shoulder screw Shoulder 1 into the HDR Tool Body Figure 2 12 Make sure the shoulder screw is in far enough to engage the thread a LFS Multichannel Pod HDR Tool Body Shoulder screws l ii Figure 2 12 Fastening the HDR Tool Body with shoulder screws 9 Using a 9 64 Allen wrench tighten the first shoulder screw until it is snug then back it off loosen it 1 2 turn Note This 1 2 turn allows the other three shoulder screws to be installed without skewing the HDR Tool Body Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 18 Multichannel Pod 10 Using the sequence designated in Figure 2 14 follow the procedure in steps 8 and 9 to install the remaining three shoulder screws Shoulder 4 0 gt
81. 1 Using a 9 64 Allen wrench follow the installation sequence designated in Figure 34 8 to install the four plunger screws Turn each plunger screw until barely snug 12 After all four plunger screws are installed go back and tighten all of them securely in the same order 13 Replace the bottom pod cover and tighten the three screws 14 Turn on the power 15 Retract the gripper using Advanced Manual Control Note After installing a multichannel head on a Multichannel Pod Hardware Setup must be updated refer to Section 4 3 Configuring a Multichannel Pod Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 16 Multichannel Pod 2 3 2 3 Installing an HDR Tool Body CAUTION To prevent damage due to electrical static discharge ESD AN wear a wrist ground strap when installing the HDR Tool Body Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek FX instrument To install the HDR Tool Body 1 Turn on the Biomek FX instrument 2 In Hardware Setup select the appropriate Multichannel Pod Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 3 Choose Change Head Note Choosing Change Head moves the pod along the D axis to 0 6 cm and extends the grippers 4 Turn off power and make sure the plastic bottom cover is removed refer to Section 2 3 2 1
82. 3 36 of syringes 3 35 D deck defined 1 8 Delta defined 6 7 setting values for the Multichannel Pod 6 11 setting values for the Span 8 Pod 6 19 Device Controller defined 1 8 devices optional overview 1 8 disposable tips installing disposable tip mandrels 3 20 removing disposable tip mandrels 3 24 E electrical connections on inside of tower detailed view 1 5 connections on outside of tower detailed view 1 5 ESD protection 3 12 F Find CD Sensitivities performing 4 27 Find Sensitivities performing 4 21 Beckman Coulter Inc firmware version viewing 6 6 fixed tips installing 3 13 removing 3 17 framing teaching instructions 5 1 precision when teaching two pods 5 1 Stacker Carousel B 29 using Manual Teach 5 15 framing fixture attaching to the head of Multichannel Head 5 5 framing shaft attaching to a probe of a Span 8 Pod 5 8 fuses changing in tower 1 14 G grippers defined 2 20 extending and retracting 6 14 framing 2 20 removing 2 23 testing framing accuracy 5 27 troubleshooting 2 21 H Hardware Setup accessing 4 2 choosing Frame Gripper 5 23 configuring a Multichannel Pod in 4 6 configuring a new head in 4 6 configuring a Span 8 Pod in 4 12 configuring the Biomek FX in 4 4 configuring the probes in 4 12 correlating the pods in 4 8 correlating the Z axes in 4 21 defined 4 1 deleting settings 4 36 Multichannel Pod properties in 4 9 performing Clot Detection Sensitivities 4 26 performing Fi
83. 30 306 13 7 Position Framed Po gt Advanced MC Teach More gt gt i Manual Teach Auto Teach Cancel Figure 5 13 Position Properties for a Positive Position ALP 4 In Name verify that the ALP is assigned a unique name 5 In Pod select the pod used to frame the desired position 6 Choose Manual Teach Manual Teaching opens with a Warning Figure 5 14 Note On the left side of Manual Teaching a list of steps required to complete the teaching process is displayed As the steps of Manual Teaching are accessed the steps are highlighted on the left Manual Teaching Warning Warning If you have snapped a continuation manually framing will erase it Press Cancel now to avoid this Clear all other positions of labware and then press Next Setup TeachZ Figure 5 14 Manual Teaching Warning Beckman Coulter Inc Framing the Biomek FX 5 17 7 Choose Next and either Figure 5 15 or Figure 5 16 appears depending on whether or not tips are already loaded onto the pod Manual Teaching TL1 Warning Figure 5 15 Manual Teaching if tips are not already loaded Manual Teaching Use currently loaded tips warning Line tips up against EAMA REESE v on position Post Figure 5 16 Manual Teaching if tips are loaded 8 In Line tips up against select the appropriate labware type of the labware placed in the
84. 50 SSA hd daR EERE ES EREEREER EREET EER eK vi Disposal of Electronic Equipment 0000s vi Laser Migihicn saan acest Res Shad eaed ade t adie din dE CeRPAeR Eee ann cance dK vi Laser Sp ciHcatons iyi cehns Cede a weg Gerun ed iN ea Eee edd ea Chee ae vi Chemical and Biological Safety lt 4 eisas see dows Hea bakes eae eid eae da eden ed vii MOVING PariS 4 626 42484624 b uated RENEE EE iene beat e EE Rena viii ClCANINS osoren OY eA i OP a E ca MOY CUE E RE viii Maintenant e 544554 64 RAPHE 64 REE E Erin E Es EERE EEE EE ERRE A EEEREN EREE RES viii Warnings and Cautions Found in this Manual 0 0 00 c eee eee eee ix Table of Contents 3 4 didi nee ee bee eed ewe ees ee een es XV List OF FIQUmeS oes ae eee ace eee ts a 2 ee ee eee xix List of ADIOS ee Cee ee eee eee ease eeee eee as XXV Biomek FX and FX P Laboratory Automation Workstations User s Manual xvi Table of Contents 1 Biomek FX Introduction 1 1 1 2 1 3 1 4 2 Multichannel Pod 2 1 2 2 2 3 2 4 2 5 2 6 3 Span 8 Pod 3 1 3 2 3 3 OVETVIEW 3 Gca can ene enan e ee aa e aa e aea ie i 1 1 1 1 1 Pod Configurations for the Biomek FX 0 000000 ee 1 2 1 1 2 Control Mod s 4c2s2civanadavieinereads Pirra n r ne Oe ndi as 1 2 Syste CoMmPoE S ares eresas piaia aat va tA ORE EE DE E GAE DA EE E 1 3 1 2 1 TOWETS e 6 RG Ae ARERR LARA READE E REREAD E ERLAR EEEE 1 4 1 222 Bridges p24 ive estas bead ee Eene EE
85. 7 DeviceControllerO g7 ShakerALPO g7 ShakerALP1 g7 SpeedPumpo of SpeedPump1 7 StirrerALPO F StirrerALP1 TiploaderO F Tiploader1 amp Digital Devices OD Simulator Camera Materials Stacker Carousels ny After changing tip types the pod must be homed and Find Sensitivities must be performed Probe Configuration Click to select which probes to configure Probes 1 8 use Fixed100 1ml s syringes Disabled Probes 1 2 3 4 5 6 7 8 V Additional Pod Settings Y Purge Settings Save Settings Restore Settings Delete Settings Last Validation Not Validated Set Validation Time Axis Limit Settings x cm cm Z cm DuL Span cm Minimum 23 79 fs 15 938 fo fo 3 Maximum 102 79 faa 6 561658 000 fia 7 Sey SaN Correlate Pods Gorrelate Z Axes Find LLS Sensitivities Pump lt none gt 7 Find GD Sensitivities BiomekFX Beckman Coulter Inc Figure 4 9 Warning on Hardware Setup that pod must be homed and Find Sensitivities performed when tip types are changed Configuring the Biomek FX in Hardware Setup 4 15 Warning x The pod must be homed after changing tip types Failing to home the pod will result in improper framing and inaccurate pipetting Figure 4 10 Warning that pod must be homed and Find Sensitivities performed when tip types are changed 4 To choose the syringe type click o
86. Detection Sensitivities is completed Configuring the Biomek FX in Hardware Setup 4 29 8 Choose Next Tips are loaded Find Clot Detection Sensitivities Choose Position appears Figure 4 28 Find Clot Detection Sens Start Select a deck position where the pod can safely determine its Clot Detection Sensitivity This position should not have any labware or other obstructions on it This position should also have 4 solid top plate Load Tips Choose Position Figure 4 28 Find Clot Detection Sensitivities Choose Position Note If any tips fail to load follow the recovery instructions displayed on Find Sensitivities Load Tips Figure 4 20 9 Select a deck position by clicking on the position Note The deck position must be an ALP with a solid ground plane for example a 1x3 Passive ALP If using a 1x1 Passive ALP or High Density 4x3 Passive ALP the deck position must have an LLS plate installed Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 30 Configuring the Biomek FX in Hardware Setup 10 Choose Next Find Clot Detection Sensitivities Test Sensitivities appears Figure 4 29 A few seconds later testing begins with a dialog box that tracks the test progress Figure 4 30 Find Clot Detection Sensitivities Start 5 Testing Progress Load Tips Initializing device for sensitivity tests Choose Position Test Sensitivities Update Settings Fi
87. EN E EREN EEES 1 7 1 2 3 Podsand Heads ecsccr correr reaa Ree ca ke ee eee eas 1 7 124 Dele 2eauveetuhe phe eaneh ne Cee eh ne Geen Shes E REECE ea 1 8 1 2 5 Protective Barriers 20seaceted see torstoasdede boca edateari 1 9 Preventive MamiCnantess 26a cde int dhe Re Sew ha REAR K RRR ORES 1 12 1 3 1 Light Curtain Mamtenanee oo 4 64 490444 84 so 46 ded4 dea twende das 1 12 MroUbleshOOuiane 5 2 eens ease eae wae Ee He DORN PALES nH Se RUS 1 13 1 4 1 Changing PUSS 66 ctnenkee rrer perk ESTEE EEEE EEN EEN 1 14 OVETVIEW cisat t os ai ainia deana aasia a aiae E Oe ene E aoina al a iaia anei 2 1 2 1 1 Main Components of the Multichannel Pod 4 2 3 DOG 4a e coed eet Ke Ewa S e eea ONS ESRD A ee SIS 2 4 2 2 1 Pod Movements ge0494auy beeeeus vente ene peat Rae ea teeeeews 2 4 22 2 Control MOdGs lt 2 4 6 02 4 0 edbnG acs kr teres bende oceans 2 4 Interchangeable Heads 23 54 24 8250n4 oon OE AOR ER ORE ORR RADE ROR ee 2 5 2 3 1 Interchangeable Pin Plates for the HDR Tool Body 2 6 2 3 2 Changing Heads cess phe ato ch des leadtak tetta aa hi paiia 2 10 GTIPPe esnie donned ied eE E TEREE dk ee kd EEEE PAE ORLA 2 19 2 4 1 Framing the Gripper aves oa eka vee sede ie nenne 2 20 Preventive Maintenance 6 occ cass oat eb ada eae ce nae eedad an aee eee wees 2 20 Troubleshooting scri pau sorten r E Gece ek IN ERRENTE EEN 2 21 2 6 1 Replacing Gripper Fingers sc0iswav sie eeasuaeesaNehweve vas 2 22 OVERVIEW oana tarere
88. Hardware Setup must be changed appropriately If the hardware configuration is not updated using Hardware Setup inaccurate liquid transfers may occur refer to the ALPs User s Manual Section 6 3 Installing and Configuring Devices Associated with the HDR Pin Drying ALP and Circulating Reservoir in Hardware Setup Also check the Tip Type Editor and make sure the settings for the Pins tip type match the manufacturer s specifications for the pins installed on the HDR Tool Body refer to the Biomek Software User s Manual Section 8 2 3 Changing Tip Properties Multichannel Pod 2 7 CAUTION To prevent damage due to electrical static discharge ESD UN wear a wrist ground strap when installing pin plates Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek FX instrument Figure 2 3 Strap portion of wrist ground strap snugly attached to the wrist Alligator clip clamped to the instrument deck base plate 1 Using Manual Control lower the pod along the D axis 0 9 cm from the maximum height and extend the gripper refer to Chapter 6 Manually Controlling the Biomek FX in Biomek Software 2 Turn off power to the Biomek FX instrument Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 8 Multichannel Pod CAUTION Carefully hold the pin plate by the edges to avoid bending or AN damag
89. Note Other Warnings may also appear depending upon the type of heads and deck configuration of the Biomek FX instrument Respond to all warnings appropriately and choose OK to continue Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 8 Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 6 In Hardware Setup choose Set D to establish a new D axis limit Note Address any warnings that appear appropriately 7 Choose Set Z to establish a new Z axis limit Note Address any warnings that appear appropriately 8 Frame the gripper according to the procedures outlined in Section 5 4 Framing the Gripper on a Multichannel Pod Note An asterisk next to the pod indicates that the head has been modified since the instrument configuration file was loaded Note Choose Change Head only when physically removing or installing a head Choosing Change Head moves the D axis to 0 6 cm and extends the gripper to remove and install a head refer to Section 2 3 2 1 Removing Heads and Section 2 3 2 2 Installing Multichannel Heads 4 3 2 Correlating the Pods CAUTION Do not use Correlate Pods in Hardware Setup without contacting a Beckman Coulter Service Engineer or Beckman Coulter Customer Technical Support To avoid collisions in a dual pod system the pods must be correlated This procedure is performed during initial setup by a Beckman Coulter Service Engineer in Hardware Setu
90. P or optional device operation is complete Note It is important to become familiar with this protected zone This reduces the possibility of causing the instrument to shut down accidentallly during operation by unintentionally violating the light curtain zone When the instrument is sitting idle or in the paused mode no violations are registered when the protective zone is penetrated This allows full access to instrument components ALPs and labware on the Biomek FX deck during a pause or system idle time Biomek FX Introduction 1 11 1 2 5 2 Protective Side Safety Shields WARNING To reduce the risk of personal injury operate the Biomek FX AN instrument only with all protective shields in place Protective shields are available for installation along the sides of the Biomek FX instrument when side light curtains are not in use Protective shields are installed by the Beckman Coulter Service Engineer if side light curtain options are not utilized If the instrument overhangs the table work surface on either side a protective bottom shield is also installed on the side of the instrument overhanging the instrument envelope Note Fither side light curtains or side safety shields must be installed by a Beckman Coulter Service Representative when the Biomek FX instrument is installed on site 1 2 5 3 Canopy The Biomek FX light curtain is blocked by a canopy that attaches to the towers and extends over the top of the instrument
91. Place the AccuFrame with the attached adaptor on the shuttle of the Stacker Carousel so that the locating pins on the bottom of the Framing Tool Adaptor align with the locating holes on the shuttle Figure B 35 First gently push the AccuFrame down into the adaptor Then place the AccuFrame with the attached adaptor on the shuttle so the locating pins on the adaptor align with the locating holes on the shuttle Figure B 35 Adding the Accuframe and Framing Tool Adaptor to the Stacker Carousel shuttle CAUTION Turn off power to the Biomek FX instrument before attaching AN or removing AccuFrame from the instrument deck 4 Turn off power to main unit before connecting the AccuFrame 5 Plug the AccuFrame into any available CAN port on the Biomek FX tower Beckman Coulter Inc Using a Stacker Carousel B 31 WARNING Make sure the light curtain is not violated by the AccuFrame cable If the light curtain is violated the framing process halts immediately gt WARNING Make sure the AccuFrame cable does not interfere with pod movement 6 Turn on power to main unit 7 Frame the Stacker Carousel according to procedures outlined in Chapter 5 Framing the Biomek FX Note The Stacker Carousel shuttle must be framed using Teach do not use Auto Teach or Manual Teach Biomek FX and FX P Laboratory Automation Workstations User s Manual xxvi
92. Remove the tip from the desired Span 8 probe probe 1 or 7 refer to Section 3 3 Interchangeable Tips 2 Screw the framing shaft onto the appropriate probe Figure 5 7 Detail A Framing shaft Detail B Figure 5 7 Attaching a Framing Shaft details 3 Install AccuFrame in the position to frame refer to Section 5 2 3 Installing AccuFrame and frame the Span 8 Pod according to the procedure in Section 5 2 4 Framing the Position Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 10 Framing the Biomek FX 5 2 3 Installing AccuFrame The AccuFrame is used during the framing process of the Multichannel Pod and the Accuframe and Framing Shaft are used to frame the Span 8 Pod After these tools are attached to the ALP and Pod respectively the framing process is completed through the software WARNING Turn off power to the Biomek FX instrument before attaching or removing AccuFrame from the instrument deck gt 1 Turn off power to the Biomek FX instrument before connecting the AccuFrame 2 Plug AccuFrame into an available CAN port on the Biomek FX tower WARNING Make sure the light curtain is not violated by the AccuFrame cable If the light curtain is violated the framing process halts immediately WARNING Make sure the AccuFrame cable does not interfere with pod movement A A Beckman Coult
93. Restoring and Deleting Settings Do not change axes limits without contacting a Beckman Coulter Service Engineer Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 33 4 4 8 Enabling Purge Settings The purge settings of a Span 8 Pod are enabled via Re Establish Air Gap and initially configured by a Beckman Coulter Service Engineer in Hardware Setup These settings should not be enabled or modified without specific instructions from a Beckman Coulter Service Engineer or Technical Support When Re establish Air Gap is enabled and configured the behavior of the Span 8 Pod is noticeably different during liquid pipetting Depending on the Cycle Threshold setting the pod moves to the Span 8 Tip Wash ALP during pipetting operations to purge liquid and air to re establish an acceptable air gap If using disposable tips they will be shucked and new tips loaded CAUTION Do not make any changes to the purge settings without AN consulting a Beckman Coulter Service Engineer Table 4 4 describes the purge settings listed in Hardware Setup for a Span 8 Pod Table 4 4 Purge Settings Property Description Re establish Air Gap When checked allows the purge settings to be enabled and configured to allow an acceptable air gap to be re established Cycle Threshold Number of aspirate and dispense cycles before the air gap is re established Post Purge Delay Amount of time in milliseconds the system waits
94. Sensitivities Update SettingS 0 0 0 ccccscesceeeeeeseeeseeceeceseeeeeeeeseecsseeeeenees 4 26 Figure 4 25 Hardware Setup Find CD Sensitivities button 0 00 0 ee eeeeeeeeeceeeceeeeneeeeeeeeeeereeaees 4 27 Figure 4 26 Find Clot Detection Sensitivities Start cssccsscssccssecssssscssscesssesensssecessasenes 4 27 Figure 4 27 Find Clot Detection Sensitivities Load Tips cccccceesceeeeeeeeeseeeeeeeseeneeeeees 4 28 Figure 4 28 Find Clot Detection Sensitivities Choose Position cccccsceesseceteeeeeeeeeeeeees 4 29 Figure 4 29 Find Clot Detection Sensitivities Test Sensitivities ccccecscecesseesseceteeeeeeeees 4 30 Figure 4 30 Find Clot Detection Sensitivities Test Sensitivities testing progress 4 30 Figure 4 31 Find Clot Detection Sensitivities Update Settings ceceeccecsseceteeeeteeeeeeeees 4 3 Figure 4 32 Save Configuration saasjssccaacssasisassancvoanssancceesnsnccaa siacevsnendacsessadextvapssaevansntiesaannzencanes 4 34 Figure 23 5 Load S CUS are ceseciaaayutesa yes ede pase a ence inset meee 4 35 Figure 4 34 Confirmation to reset stored Configuration c ceceesesseeeeeeeceeeceseeeeeeseeeeeeereeaees 4 35 Figure 4 35 Delete Settings accscaaccascuecoseesvenenesantenagsxeutocseansnoccsvancaiunciaennmeacneaananaeseareaeunsemantes 4 36 Figure 4 36 Confirmation to delete stored configuration ssssssssessssssesesstsetsrsstsesseseesssserees 4 36 Basie 5 1 AccuF
95. Setup to relate information about the configuration single arm or dual arm of the pod s on the system to the software and to specify the communications port to which it is connected To configure the Biomek FX instrument 1 From Hardware Setup select Biomek FX from the left pane The configuration view appears in the right pane Figure 4 3 2 Make sure the serial number listed in Hardware Setup corresponds to the serial number on the Biomek FX instrument 3 Choose the appropriate Port 4 Iftwo pods are located on the instrument check This is a dual armed system 5 Choose the appropriate type for Left Pod Type and Right Pod Type from the drop down menus Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 5 6 Configure the left and right pods appropriately according to the instructions in Section 4 3 Configuring a Multichannel Pod or Section 4 4 Configuring a Span amp Pod 7 Choose Accept Hardware Setup closes Note Accept must be chosen after the instrument has been configured to allow Hardware Setup to accept the configurations However other devices may be configured when the Biomek FX instrument is configured and Accept may be chosen after all devices have been configured Note An asterisk next to the device indicates the device has been modified since the instrument was loaded Biomek Hardware Setup Reconnect ff Home All Axes db Add Device s R Device W Acce
96. X and FX P Laboratory Automation Workstations User s Manual 3 22 Span 8 Pod 2 While holding the disposable tip mandrel in one hand push the tubing down onto the disposable tip mandrel with the other hand Make sure the disposable tip mandrel is secure to prevent air entering the system and fluid leaks around the tip Note The ends of the tubing may become damaged or stretched after repeated tip installation and removal It may be necessary to cut a small portion 1 2 of tubing off the end prior to attaching to ensure a tight fit Note Since the tubing is Teflon it may be difficult to hold Holding the tubing with a Kimwipe may prevent slipping while pushing the tubing down onto the tip 3 Gently push the tubing and disposable tip mandrel up into the tip interface 4 Pass the collar up over the disposable tip mandrel to the tip interface Figure 3 14 Probe Tip Interface Disposable Tip Mandrel Collar Figure 3 14 Passing collar over a disposable tip mandrel 5 Screw the collar clockwise into the tip interface until a tight fit is achieved Note Ifthe tip mandril is not held the syringe line twists at the top of the probe assembly and kinks Note Each week check that disposal tip collars are securely fastened to the tip interface Disposal tips may not shuck if collars are loose Beckman Coulter Inc Span 8 Pod 3 23 6 Pass the tip
97. a syringe dictates the maximum amount of fluid aspirated into and dispensed from the tubing for each of the eight Span 8 probes and the speed at which the fluid flows Syringes are available in three sizes e 250uL 500uL ImL Two different sizes of syringes can be used in the pump assembly at the same time in order to perform pipetting operations at different volumes during a single method Note Probes 1 through 4 must all use the same size syringe and probes 5 through 8 must all use the same size If two different sizes of syringes are required to ensure pipetting accuracy within a single method or the size of syringes installed in the Biomek FX instrument changes frequently new techniques for each size of syringe should be created This is accomplished by copying the Span 8 techniques to create multiple sets of techniques and by editing the Cutoff Velocity and other pipetting paramaters in those techniques to support the size of syringe s installed Note Refer to the Biomek Software User s Manual Section 10 9 Modifying Saved Techniques for more information on copying techniques and Section 0 4 8 Overriding Liguid Type Values for more information on editing liquid types Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 32 Span 8 Pod Beckman Coulter Inc 3 4 2 3 Assembling and Installing Syringes Syringes are shipped in two pieces e Plunger e Glass casing Once assembled the
98. affects the sensitivity of the light curtain and adversely impact its effectiveness Typical lab dress such as lab coats and latex gloves do not degrade light curtain operation however it is advisable to test the impact of the all lab dress on light curtain sensitivity before operating the Biomek FX Verify lab dress impact on light curtain sensitivity as follows Use Manual Control in the software and insert the material no more than 1 past and approximately 21 above the light curtain panel Make sure the solid amber indicator light changes to blinking amber gt gt gt p WARNING To prevent injury use proper decontamination procedures CAUTION If service is required contact a Beckman Coulter Service Engineer WARNING Do not remove tower covers to access electrical wiring or fuses Change only the fuses that are accessed from the outside of the instrument without removing covers Contact a Beckman Coulter Service Engineer if further access is required WARNING Turn off and unplug power to the instrument before changing fuses Failure to do so can cause electrical shock or equipment damage CAUTION Use labware and tip combinations labeled as Limited with caution Tips could become wedged inside labware picking the labware up with the probe when the probe is raised CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod CAUTION D
99. ake sure the front of the framing fixture is to the front of the instrument and the framing guides are to the back and left of the instrument Figure 5 3 2 Gently pull the framing fixture to the front and right Verify the framing guides are touching the mandrels 3 Tighten each thumb screw by turning to the right until the framing fixture is firmly attached to the head The pod is now ready for framing Back of framing Front of framing fixture fixture Framing probe UN ORUE XJ Thumbscrews Four thumb screws attach the framing fixture to the Multichannel head Figure 5 3 Framing Fixture 4 Install AccuFrame in the position to frame refer to Section 5 2 3 Installing AccuFrame and frame the Multichannel Pod according to the procedure in Section 5 2 4 Framing the Position Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 6 Framing the Biomek FX 5 2 2 2 Attaching the Framing Fixture to the HDR Tool Body of a Multichannel Pod When positioning the HDR Framing Fixture Figure 5 4 the framing probe must be pointed down and away from the mandrels of the head From the front perspective the framing guides on the framing fixture must be to the back and left CAUTION When using Manual Teach to frame a position with the HDR AN Tool Body move the D axis all the way down to prevent the gripper from crashing
100. al P21 Tubes2 Cancel Figure 4 20 Find Sensitivities Load Tips error Start Select a deck position where the pod can safely determine its LLS Sensitivity This position should not have any labware or other obstructions on it This position should also have a solid top plate Tu Pos ial j BRI Pos3 Tubes2 P12 has been selected where the pod can determine its LLS sensitivity Load Tips Choose Position Figure 4 21 Find Sensitivities Choose Position Beckman Coulter Inc gt onfiguring the Biomek FX in Hardware Setup 4 25 9 Select a deck position by clicking on the position Note The deck position must be an ALP with a solid ground plane for example a 1x3 or 1x5 Passive ALP or a 4x4 High Density Passive ALP If using a 1x1 Passive ALP it must have an LLS plate installed 10 Choose Next Find Sensitivities Test Sensitivities appears Figure 4 22 A few seconds later Figure 4 23 appears indicating testing is in progress Testing Progress Initializing device for sensitivity tests Figure 4 22 Find Sensitivities Test Sensitivities Testing Progress Determining maximum sensitivities for each probe Figure 4 23 Find Sensitivities Test Sensitivities indicating testing progress Biomek FX and FX P Laboratory Automation Workstations User s Manual Configuring the Biomek FX in Hardware Setup sensitivities have been completed liquid level sensin
101. annel Pod main components esceesseeeceseceseceeeeseeeeeeeeeeseceaeeneeeneeeeees 2 3 Figure 2 3 Wrist ground strap attached to the deck ss s sensessesesssesesessesestsestssessesseseesesseserse 2 7 Figure 2 4 Fastening the pin plate to the HDR Tool Body sssessesssssssseseesesseseesesseressseseesesse 2 8 Figure 2 5 Wrist ground strap attached to the deck weiss ceccsssvsdesstuissiacesssneneastonsenetavseraenisnqenesone 2 10 Figure 2 6 Bottom cover removed from the pod siocssscssucdsnssvoresosssatspsasctetoadunionsdveaieneaieresnednass 2 11 Figure 2 7 Plunger screws and shoulder screws removed from multichannel head 2 12 Figure 2 8 Sequence for removing four plunger screws and four shoulder screws on all multichannel heads d0scaisasaecmaxaronesnanse Geaiaaestnaraeiseeree tie aoa 2 13 Figure 2 9 Chamfer location on a Multichannel head 0 0 0 0 cccecsceceseceeeceeeeeeseeeeseceeeeeseeeeseeees 2 14 Figure 2 10 Sequence for installing four plunger screws and four shoulder screws on all multichannel heads ssiccasasaaensvsyscussaups aasuandeancdunssdeseeia aaee EEE EE Eaa tances pioanveds 2 15 Figure 2 11 Chamfer locations on the HDR Tool Body sssssessesssssssesssssersessrsrrsrssesersssserees 2 16 Figure 2 12 Fastening the HDR Tool Body with shoulder SCrewS c eccesceseeereeteeeeeeereeeees 2 17 Figure 2 13 Sequence for installing four plunger screws and four shoulder screws on the HDR To
102. any warrants that the products sold under this sales agreement shall be free from defects in workmanship and materials for one year after delivery of the products to the original Purchaser by the Company and if any such product should prove to be defective within such one year period the Company agrees at its option either 1 to correct by repair or at the Company s election by replacement any such defective product provided that investigation and factory inspection discloses that such defect developed under normal and proper use or 2 to refund the purchase price The exceptions and conditions mentioned above are as follows a Components or accessories manufactured by the Company which by their nature are not intended to and will not function for one year are warranted only to reasonable service for a reasonable time What constitutes a reasonable time and a reasonable service shall be determined solely by the Company A complete list of such components and accessories is maintained at the factory b The Company makes no warranty with respect to components or accessories not manufactured by it In the event of defect in any such component or accessory the Company will give reasonable assistance to Purchaser in obtaining from the manufacturer s own warranty c Any product claimed to be defective must if required by the Company be returned to the factory transportation charges prepaid and will be returned to Purchaser with tran
103. ation however when the supply container runs out of system fluid refer to Section 3 5 1 System Fluid the container must be replaced Note Supply containers can be reused when they are filled with a system fluid recommended for the Span 8 Pod refer to Section 3 5 1 System Fluid Overflow Connection Overflow for the Speed Pump is directed back into the supply container This connection is used only when a Speed Pump is installed Supply Connection px The supply line runs ne Quick Disconnect from the supply Su Fittings container to the Span 8 as pumps or to the Speed Los Pump JS 3 8 Tubing Figure 3 29 Supply container 3 5 3 1 Replacing a Supply Container The supply container attaches to the Biomek FX instrument via quick disconnect fittings and 3 8 tubing on its cap To replace a supply container 1 Detach the supply and overflow connections by pressing in and pulling up on the tabs on the quick disconnect fittings Figure 3 29 2 Unscrew the cap on the supply container by turning it counterclockwise 3 Lift the cap straight up and off of the bottle until the internal supply tubing clears the top of the supply container 4 Remove the empty supply container Note Supply containers can be refilled with system fluid degassed and then reattached to the Biomek FX instrument Span 8 Pod Position a new supp
104. ation appears Figure 4 32 Name MultichannelPodSettings Description Figure 4 32 Save Configuration 3 If desired enter a description of the configuration 4 Choose OK The settings are saved Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 35 4 5 2 Restoring Pod Settings To load the saved settings 1 In Hardware Setup select the appropriate Pod from the left pane to access the configuration area in the right pane Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 2 Choose Restore Settings Load Settings appears Figure 4 33 Load Settings Settings Settings Information MultichannelPodS ettings1 Validation Time Not Validated a Serial Number Head Type 96 Mandrel 200 pL Head Desired settings to Axis Limit Settings load are selected here Y 5 90 33 20 D 58 43 229 00 Speed Limit 100 Additional Roving Height o 75cm Additional Timeout 25 00 Do not move to Z max men roving Gripper Settings Gripper Offset zi cea Figure 4 33 Load Settings 3 Select the desired settings 4 Choose OK The following Confirm appears Figure 4 34 Q Are you certain you want to reset your pod configuration to the values in the stored configuration MultichannelPodSettings1 Click Yes to modify the current pod configuration Click No to retain the current pod configuration
105. ations in order to achieve optimal performance and maximum life expectancy CAUTION Do not run syringes more than a few cycles without fluid in AN the syringes Syringes should not actuate while dry unless the system is being primed refer to Section 3 5 5 2 Priming the System with System Fluid When priming the system the syringes should cycle at maximum volume as few times as possible in order to flood the syringe with system fluid as quickly as possible Note A cycle is one up or down motion of the plunger in a syringe Refer to Section 3 4 2 3 Assembling and Installing Syringes for identification of the parts of a syringe Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 38 Span 8 Pod 3 5 Liquid System The Span 8 Pod uses a liquids system to provide a vacuum for pipetting operations as well as for tip washing and bulk dispense The Span 8 liquid system includes e System Fluid Acts as a medium for syringes to apply a vacuum used during pipetting operations and is used to wash tips in the Span 8 Tip Wash ALP refer to Section 3 5 1 System Fluid Tubing System fluids are moved through the tubing refer to Section 3 5 2 Tubing e Supply Container Source of system fluid for the Span 8 Pod refer to Section 3 5 3 Supply Container e Waste Container Final destination of fluids dispensed to the Span 8 Tip Wash ALP and overflow from the Span 8 pumps refer to Section 3
106. cations and electrical connections to the base unit and moves the probes in the D Z and Y axes for liquid handling functions Figure 3 1 and Figure 3 2 The main components of a Span 8 Pod are Figure 3 1 e Probes Section 3 2 Move independently in the Z axis and pipette independently in the D axis with the assistance of the pump assembly Hold the tip interface for fixed or disposable tips both Liquid Level Sensing LLS capable and non LLS capable used to perform liquid handling operations refer to Section 3 3 Interchangeable Tips Figure 3 1 and Figure 3 2 probes move in the Y Z S pan and D ispense axes Figure 3 1 Interchangeable Tips Section 3 3 Fixed or disposable tips that are attached to the Span 8 probes used to perform microplate to microplate test tube to microplate and test tube to test tube liquid transfers e Pump Assembly Section 3 4 Houses the individual pumps and syringes which control the flow of system fluid to and from each of the eight probes by controlling the D axis Figure 3 1 Figure 3 3 located between the towers at the back of the Biomek FX instrument Figure 3 1 refer to Section 1 2 System Components e Liquid System Section 3 5 Stores and transports system fluid used to provide a vacuum for pipetting wash tips and perform bulk dispense operations e Speed Pump Section 3 6 Optional device which propels system fluid through fixed tips at a
107. channel Pod to a Safe Roving Height Use Move Z Max in Advanced Manual Control for a selected Multichannel Pod to move it to its highest configured height This helps to avoid collisions when moving the pod around the deck manually Note The possibility of collisions is not completely eliminated by this command for example if the gripper is extended and holding a microplate and the pod is moved over a tip box on a tiploader a collision could occur 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Multichannel Pod appears Figure 6 9 4 Choose Move Z Max 5 Choose Close to close Advanced Manual Control 6 Choose Exit to close Manual Control Biomek FX and FX P Laboratory Automation Workstations User s Manual 6 10 Manually Controlling the Biomek FX in Biome 6 4 3 Performing Relative Moves for the Multichannel Pod Relative moves allow the pod to move from its current location to anywhere on the deck Relative moves are created in the Vector Builder using the Delta values or the vector can be manually edited Note Use Auto Clear when the values in the Movement Vector fields must be reset to zero after the move is performed Turn off Auto Clear to retain the values after the move has occurred Choose Clear to set the values back to zero at any time To move a pod relative to its current posi
108. ct f Home All Axes b Add Device Remove Device J Accept gt lt Cancel Biomek FX SN None Cera Number rsd j AccuFrame 96 Podi P ort X 8 Pod Simulate Devices I This device has a Bar Code Reader 7 DeviceController0 oF ShakerALPO oF ShakerALP1 A None x oF SpeedPumpo oF StirrerALPO B None z oF StirrerALP1 7 Tiploaderd C None v g7 Tiploader1 Digital Devices 0 None OF Simulator E Camera OF Materials Stacker Carousels BiomekFX Figure B 34 Hardware Setup showing the configuration view for a Stacker Carousel 2 Enter the correct Serial Number to correspond to the serial number on the Stacker Carousel 3 Choose the appropriate Port 4 Check This device has a Bar Code Reader when a bar code reader has been attached to the Stacker Carousel 5 Choose the appropriate stacker type for each stacker on the carousel Note Up to four stackers may be attached to the Stacker Carousel Stackers are identified on the Stacker Carousel as A B C or D Make sure the correct stacker type is selected for each stacker on the carousel Stacker types may be Stacker 10 Stacker 20 or Stacker HD 6 Choose Accept Hardware Setup closes Note Accept must be chosen after the Stacker Carousel has been configured to allow Hardware Setup to accept the configuration Biomek FX and FX P Laboratory Automation Workstations Us
109. d e Positive value Z up motion of e Negative value Z down the probes motion of the probes e Positive value D aspirating e Negative value D dispensing motion of the probes motion of the probes e Positive Span widening e Negative Span narrowing motion between the probes motion between the probes Biomek FX and FX P Laboratory Automation Workstations User s Manual 6 20 Beckman Coulter Inc Manually Controlling the Biomek FX in Biomek Soj 6 5 4 Performing Absolute Moves for the Span 8 Pod Absolute Move allows the pod to move to a specific coordinate position in the workspace of the instrument Use Absolute Move when the coordinates of the desired position are known Note When an Absolute Move is entered the values displayed in Movement Vector reflect the relative move required to physically move the pod to the desired position Make sure the physical location of the pod is not changed between the time the vector is built and the time the Go button is pressed 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Span 8 Pod appears Figure 6 12 4 Choose Absolute Move Absolute Move appears Figure 6 14 Absolute Move x Current Position x 94 79 cm z 4253 4 253 4 253 4 253 4253 4 253 4 253 4 253 cm v fis cm po 0 0 a 0 0 a 0 uL Span 18 84 mm
110. d by the Speed Pump without actuating the pumps or syringes When the Span 8 Pod needs to perform a tip wash in the Span 8 Tip Wash ALP with the assistance of a Speed Pump a Device Action step must be inserted in the method prior to the Span 8 Wash Tips step and the Use speed pump option must be selected The Use speed pump option facilitates setting the valves in the pumps to bypass so the pumps and syringes are not used refer to the Biomek Software User s Manual Section 23 6 1 7 Configuring the Device Action Step for a Positive Position ALP FX NX only Note The volume of fluid used during the tip wash and the number of wash cycles completed are set in the Span 8 Wash Tips step configuration refer to the Biomek Software User s Manual Section 19 8 Span 8 Wash Tips Step Note The Speed Pump is used only during the tip wash process for fixed tips Disposable tips cannot be washed in the Span 8 Tip Wash ALP Figure 3 33 Speed Pump front view Note The Speed Pump must be positioned in line between the supply container the source of the system fluid and the pumps Beckman Coulter Inc Span 8 Pod 3 49 WARNING Debris resulting from installing a fitting in the supply UN container may clog the tubing f WARNING Using a dirty supply container may clog the tubing Located on the back of the Speed Pump are Figure 3 34 e Two CAN communication ports Note A Controller Area Network
111. dvanced Manual Control with the Multichannel Pod 2 Orient the gripper finger so that the front of the gripper finger faces the outside and gripper pads face the inside of the Multichannel Pod Figure 2 16 3 Grasping both sides of the gripper finger push the finger straight up into the Multichannel Pod Note A click can be heard and felt when the gripper fingers are seated in the Multichannel Pod 4 Pull down on the gripper finger to ensure that it is fully seated in the pod 5 Reframe the Multichannel Pod refer to Section 5 4 Framing the Gripper on a Multichannel Pod Beckman Coulter Inc Span 8 Pod 3 1 Overview The Span 8 Pod is a self contained working unit installed on the Biomek FX instrument Figure 3 1 The Span 8 Pod is a liquid handling tool capable of performing liquid transfers from test tubes and large pieces of labware to smaller pieces of labware or vice versa The Span 8 Pod can also perform liquid level sensing LLS during liquid transfers when liquid level sensing enabled tips are attached to the probes gt FX The Span 8 Pod can also perform clot detection CD during liquid transfers when conductive tips are attached to the probes Note Conductive disposable tips are identified as LLS in the Tip Type Editor Fixed tips are also conductive The Span 8 Pod performs liquid transfers using a series of eight probes that can move independently in the Z axis pipette independently
112. e Disposable x tips Disabled Probes Click to select which probes to configure fi mL X syringes 1 2 3 4 5 6 7 3 J Accept gt lt Cancel Save Settings Biomek Hardware Setup i Reconnect fF Home All Axes Gp Add Device me Remove Device Restore Settings Delete Settings s Last Validation Not validated Set Validation Time Axis Limit Settings x om Minimum 23 79 BE ik DE Maximum 102 79 Set D ul 0 Span cm ce 14 7 Y cm 5 5 Z cm 15 938 ai 40 6 56165 1000 SEEN E Gorrelate Pods i Additional Pod Settings Purge Settings Pump lt None gt x Find GD Sensitivities Find LLS Sensitivities Gorrelate Z Axes f Select a Speed Pump for the Span 8 Pod here 10 Beckman Coulter Inc Figure 4 15 Selecting a Speed Pump for a Span 8 Pod for use Choose Accept Hardware Setup closes The Speed Pump is configured Note The operation of the Speed Pump is controlled through Manual Control prior to running a method refer to the Biomek FX and FX P Laboratory Automation Workstations User s Manual Section 6 6 Using Advanced Manual Control to Manually Control Speed Pumps or through a Device Action step during a method run refer to the Biomek Software User s Manual Section 23 6 Device Action Step or by selecting the Speed Pump option in a T
113. e or Cancel to choose a more suitable position E Cancel Figure 6 16 Warning to address if a Span 8 Tip Wash ALP is not defined Beckman Coulter Inc ally Controlling the Biomek FX in Biomek Software 6 23 5 Ifnecessary take the appropriate action to address the warning and choose OK Information appears Figure 6 17 Information x G When the intake is clear of bubbles press OK Figure 6 17 Information 6 Follow the instructions displayed in Information When completed choose OK The air is purged from the syringes and tubing 7 Choose Close to close Advanced Manual Control 8 Choose Exit to close Manual Control Biomek FX and FX P Laboratory Automation Workstations User s Manual 6 24 Manually Controlling the Biomek FX in Biomek Soi 6 6 Using Advanced Manual Control to Manually Control Speed Pumps Use Advanced Manual Control for a selected Speed Pump to Turn on the Speed Pump Turn off the Speed Pump To turn on a Speed Pump 1 Choose Instrument gt Manual Control Manual Control appears 2 Choose Advanced Controls 3 Select the desired Speed Pump Advanced Manual Control for the selected Speed Pump appears Figure 6 18 Advanced Manual Control SpeedPump1 Command On ha Figure 6 18 Advanced Manual Control for a selected Speed Pump From Command choose On Choose Go nH Nn A Choose Close to close Advanced Manual Control 7
114. e 3 3 Labware and Tip Compatibility Tip Type Labware Fixed Fixed Disposable Disposable P1000 100 60 AP96_200 uL AP96_20 uL Flat 96 R R R R R Deep 96 R R R L R Flat 384 P R L R N Deep 384 L R L La N BCTubeRack 10mm R La L L R x75 mm BCTubeRack 12mm R L R R R x75 mm BCTubeRack 13mm R L R R R x100 mm BCTubeRack 15mm R L R R R x100 mm R Recommended L Limited Access due to height and or width restrictions see Caution above N No Access a The tip fixed or disposable is too short to allow the tip to access fluid in the bottom of the test tube Figure 3 3 and Figure 3 4 b Fixed 100 tips are designed to fully access test tubes while Fixed 60 tips are designed to increase reliability in accessing high density 384 well microplates c The maximum diameter of the disposable tip is too wide to allow the tip to fully access the labware Figure 3 3 Figure 3 4 and Figure 3 5 Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 12 Span 8 Pod Maximum diameter gt Figure 3 3 Fixed tip height and width restrictions i Maximum pH diameter Height gt Figure 3 4 Disposable tip height and width restrictions Beckman Coulter Inc Span 8 Pod 3 13 Tip shuck tube Only the disposable
115. e Firmware Version Get Version shows the current firmware version for installed devices pods and main firmware To view the firmware version 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Get Version The firmware version displays in an Information dialog similar to Figure 6 8 Information Ed Master Controller 2 37 Left arm XY Board 0 7 15 BL 1 5 2 Left arm ZD Board 0 7 15 BL 1 5 2 Right arm XY Board 0 7 15 BL 1 5 2 Right arm ZD Board 0 7 15 BL 1 5 2 Figure 6 8 Firmware version information 3 To close Information choose OK 6 3 5 Accessing Advanced Manual Control To access Advanced Manual Control 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select a device from the menu Advanced Manual Control for the selected device appears 6 4 ly Controlling the Biomek FX in Biomek Software Multichannel Pod Use Advanced Manual Control for the Multichannel Pod to Note Many activities performed in Advanced Manual Control are the same for Using Advanced Manual Control with the Viewing the Current Position of a Multichannel Pod Section 6 4 1 Moving a Multichannel Pod to a Safe Roving Height Section 6 4 2 Performing Relative Moves for the Multichannel Pod Section 6 4 3 Performing Absolute Moves for the Multichannel Pod Section 6 4 4 Extending and Retract
116. e Speed Pump with a Span 8 Pod Biomek Hardware Setup Reconnect fF Home All Axes db Add Device Remove Device lt Accept gt lt Cancel El Biomek FX SN None SpeedPump1 does not support any configuration options iM AccuFrame 96 Pod1 8 Pod2 o Devices 47 DeviceControllerO 47 ShakerALPO oF ShakerALP1 lt 7 SpeedPumpo fame SpeedPump1 oF StirrerALPO oF StirrerALP1 lt 7 TiploaderO g7 Tiploader1 Digital Devices E Simulator E Camera E Materials Stacker Carousels Figure 4 13 A Speed Pump has been added to the Hardware Setup OR 5 From Hardware Setup right click on Devices Figure 4 14 6 Select Add Device A Speed Pump has been added to the Hardware Setup Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 19 7 Select SpeedPump HW Address 00 for the first Speed Pump added to the Biomek FX instrument Select SoeedPump HW Address 01 for the second Speed Pump added to the instrument and SpeedPump HW Address 02 for the third Note Only three Speed Pumps can be added to the Biomek FX instrument at one time Biomek Hardware Setup Reconnect fi Home All Axes G Add Device me Remove Device J Accept gt Cancel Biomek FX SN None j AccuFrame 96 Podi 8 Podz E vice SpeedPump HW Address 01 Remove Device PositivePositionALP HW Address 00 Sel PositivePositionALP HW Address 01 speceramnpe _ s
117. e and direction of motion applied to the pod Use the Vector Builder buttons Figure 6 10 to add positive or negative values to the Movement Vector for the pod Each time a Vector Builder button is pressed the Delta value for that axis is added or subtracted from the appropriate Movement Vector for the pod With the point of reference at the front and center of the unit positive and negative values for the pod are as follows Vector Builder g a ie ran efan sie Squeeze Aspirate lt gt UnS queeze Dispense Figure 6 10 Vector Builder e Right Positive value X right e Left Negative value X left motion of the pod e Fwd Positive value Y forward motion of the pod e Up Positive value Z up motion of the probes e Squeeze Aspirate Positive value D squeezing of the gripper or aspirating motion of the probes e Widen Positive Span widening motion between the probes motion of the pod Back Negative value Y back motion of the pod Down Negative value Z down motion of the probes Unsqueeze Dispense Negative value D unsqueezing of the gripper or dispensing motion of the probes Narrow Negative Span narrowing motion between the probes ally Controlling the Biomek FX in Biomek Software 6 13 6 4 4 Performing Absolute Moves for the Multichannel Pod Absolute Move allows the pod to move to a specific coordinate position in the
118. e applied to the tips each time a directional button is selected Figure 5 19 Note The default Delta value is 0 05 cm If the tips are a considerable distance above the ALP increase the distance traveled by increasing the Delta value maximum setting is 1 0 cm If the tips are almost to the desired location reduce the Delta value minimum setting is 0 005 cm Note Since the X and Y axes were framed previously it is safe to move the pod in the X and Y axes if it makes framing the pod in the Z axis easier Beckman Coulter Inc Framing the Biomek FX 5 21 20 Select the directional button representing the motion required to physically move the tip down into the wells of the microplate until the tips touch the bottom of the wells Note Each time a directional button is selected the pod and tips move the distance specified in Delta in the indicated direction Note The tips can be can physically positioned over the wells of a microplate using e the directional buttons in Manual Teach e the directional keys on the keyboard e the directional keys on the numeric keypad The directional buttons displayed in Manual Teach parallel the keys on the numeric keypad More specifically Fwd correlates to the 1 on the numeric keypad while Down is found on the 2 Left is found on the 4 Right on 6 Up on 8 and Back on 9 21 Select Finish The pod moves up to its maximum height in the Z axis
119. e cautionary procedures as defined by your safety officer when using flammable solvents in or near a powered up instrument e Observe the appropriate cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials Note Observe all warnings and cautions listed for any external devices attached or used during operation of the Biomek FX Refer to applicable external device user s manuals for operating procedures of that device Biomek FX and FX P Laboratory Automation Workstations User s Manual viii Safety Information Moving Parts To avoid injury due to moving parts observe the following e Never attempt to exchange labware reagents or tools while the instrument is operating e Never attempt to physically restrict any of the moving components of the Biomek FX e Keep the Biomek FX work area clear to prevent obstruction of the movement Cleaning Observe the cleaning procedures outlined in this user s manual for the Biomek FX Prior to cleaning equipment that has been exposed to hazardous material e Appropriate Chemical and Biological Safety personnel should be contacted e The Chemical and Biological Safety information contained in this user s manual should be reviewed Maintenance Perform only the maintenance described in this manual Maintenance other than that specified in this manual should be performed only by Beckman Coulter service representatives Impo
120. e of the four sides of a microplate Note For maximum scanning reliability it is recommended that labels be applied to the narrow sides of the microplate This label orientation accommodates scanning of skewed labels and requires less adjustment of the bar code reader position A WARNING CLASS II LASER PRODUCT THIS PRODUCT CONFORMS TO APPLICABLE REQUIREMENTS OF 21 CFR 1040 AT THE DATE OF MANUFACTURE Integrating the bar code reader requires e Attaching Bar Code Reader Bracket Section B 3 1 Attaching Bar Code Reader to Shuttle Section B 3 2 e Positioning Bar Code Reader Section B 3 3 Applying Power to the Bar Code Reader Section B 3 4 e Aligning the Laser Beam Section B 3 5 B 3 1 Attaching Bar Code Reader Bracket Attach the bar code reader mounting bracket to the bar code reader as follows 1 Turn off the Stacker Carousel power switch 2 Using a Phillips head screwdriver fasten the bar code reader Microscan MS710 to the bracket using two M4 x 6 flat head screws Figure B 14 M4 x 6 flat Bar code reader Bar code reader mount Alignment bracket adjustment screws Bar code reader cable lamp og Figure B 14 Bar code reader bracket Biomek FX and FX P Laboratory Automation Workstations User s Manual B 14 Using a Stacker Carousel B 3 2 Attaching Bar Code Reader to Shuttle Attach the bar code
121. e pendant to move the shuttle with the labeled microplate through the laser beam The laser beam should pass over all vertical bars on the bar code label simultaneously Note Correct and incorrect laser label alignments are shown in Figure B 29 Correct laser alignment Incorrect laser __ i i i H are alignment Figure B 29 Bar code reader Laser beam position over bar code label 7 Using a Phillips head screwdriver loosen laser alignment adjustment screws to adjust laser alignment as necessary Figure B 30 Retighten screws when adjustment is complete Laser beam Laser alignment tment screws Figure B 30 Adjust laser alignment as necessary Note The bar code reader is pre configured to read a variety of bar codes Biomek FX and FX P Laboratory Automation Workstations User s Manual B 24 Using a Stacker Carousel CAUTION If labware other than that specified in the Stacker Carousel User s Manual is used an increase in bad reads or no reads may occur CAUTION If a label is applied by any means other than the Beckman UN Coulter Print and Apply device an increase in bad reads or no reads may occur 8 If label needs to be applied to the microplate it is recommended that a Beckman Coulter Print and Apply device be used to position the label on the microplate properly Figure B 31 0 56 0 25 in 1 45 0 25 in
122. e pod moves when Go is selected Figure 6 9 Overview Advanced Manual Control for a Multichannel Pod Note See Table 6 1 for further descriptions of fields Biomek FX and FX P Laboratory Automation Workstations User s Manual Manually Controlling the Biomek FX in Biomek Sof Refer to specific subsections for instructional use Table 6 1 Advanced Manual Control selection areas for the Multichannel Pod Area Absolute Move Description Builds a vector to an absolute coordinate from the current coordinate Auto Clear When checked each time Go is selected the Movement Vector resets to the 0 vector no movement Note Auto Clear is on by default Clear Sets movement vector entries to 0 Current Position Current location of the pod after the pod has been homed D Units Sets the D axis units Note Choose cm to set squeeze units or uL to set aspirate units Delta Sets the magnitude of change the Vector Builder applies to the Movement Vector for each axis Extend Gripper Extends the grippers Home D Moves D axis to home position Home Z XY Moves Z and then X and Y axes to home position Move Z Max Moves the pod to highest configured height Movement Vector The amount of movement that occurs when Go is selected Movement vectors are relative to the current position Retract Gripper Retracts the grippers Speed Sets the speed of
123. e probes are about to go down to the washstation Press OK to continue or Cancel to abort 3 Cancel Figure 6 6 Warning that the Span 8 probes are about to go down to the washstation Beckman Coulter Inc ally Controlling the Biomek FX in Biomek Software 6 5 Information Ea Ci When the intake is clear of bubbles press OK Figure 6 7 Information for a Span 8 Pod Note Choosing Home All Axes homes all of the axes for all pods 3 Choose OK on each Warning and Information after confirming that the actions have been addressed appropriately 6 3 2 Moving a Pod to a Specific Deck Position Use Manual Control to easily move the pod to a specific deck position Manual Control moves the pod to the top of the Z axis then centers it over the selected position To move a pod to a specific deck position 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 In Click on a position to move select the desired pod 3 Click on the desired deck position on the Manual Control Deck Display Figure 6 2 6 3 3 Stopping a Pod To stop a pod once a movement has started 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 In Click on a position to move select the desired pod 3 Choose Stop Biomek FX and FX P Laboratory Automation Workstations User s Manual 6 6 Manually Controlling the Biomek FX in Biome Beckman Coulter Inc 6 3 4 Viewing th
124. ecneeeaeeeeeeeeeeaeeeaeeneeess 6 2 Figure 6 2 Manual Control sxiovessnscencantaaocesscacencacseesoncmsinansaseadsasasttasenamsteasseastaesseqneecavareacnsmanens 6 2 Figure 6 3 Warnings must be addressed before homing process begiNS ccsceeteeteeeteees 6 4 Figure 6 4 Warning to address if the system is dual pod ec eeceeceseeseeereeeeeeeeceseceseeneeeneeeeeees 6 4 Figure 6 5 Warning to address if a Span 8 Tip Wash ALP has not been added to the deck RTI TPE UU AL CO 0 gogo EE E eaten aaa aca E 6 4 Figure 6 6 Warning that the Span 8 probes are about to go down to the washstation 6 4 Figure 6 7 Information for a Span 8 PO csxcccsccarcsesenconcesisanstevcneassecnsanesacesatavaadeacestaedseateayeeweeenteves 6 5 Figure 6 8 Firmware version information cjash nseceigrvnutdawiaunseatenunsanegnsdardauvaiatamtendalervanscduinmeanerves 6 6 Figure 6 9 Overview Advanced Manual Control for a Multichannel Pod ce eeeeeeceteeeeeeee 6 7 Fig re 6 10 Vector Builder sscesaccpancnestvivinnataceusynesudeunssaniessinasasenyeatuenselssyiuncbineinsassuesieheamelsnieets 6 12 Figure 6 11 Enter Absolute Move Coordinates a ccsiscisatosiecsaasceaceeinaiacastedgaeisieaineiabianavanciencaiade 6 13 Figure 6 12 Overview Advanced Manual Control for a Span 8 ccecceseeeseeseeeeeeeeeeeeeeeeeees 6 15 Figure 6 13 Vector Builder for the Span 8 Pod s sssesssssessessrsessssseessssrsssseseesesseseessssensessesee 6 19 Figure 6
125. ectaussacees EESE Ria 4 16 Fig ur 412 New DEVICES axcasutuseetyvetaterenecaceneeeereten anne nance 4 17 Figure 4 13 A Speed Pump has been added to the Hardware Setup cecsseeseeseeereeereeeees 4 18 Beckman Coulter Inc List of Figures xxi Figure 4 14 Right clicking on Devices to add a Speed Pump in Hardware Setup 0 0 00 0 4 19 Figure 4 15 Selecting a Speed Pump for a Span 8 POdsssisicesascascassicoesiadssdeinavsendenssaatecnatendaneass 4 20 Figure 4 16 Find Sensitivities Start sessessssseessessessecssesssesorsseesoesorsseesseserssressessesseessessossees 4 22 Figure 4 17 Find Sensitivities Start tip detection cc cc ccccsccceseceeeeeeseeeeecseceeeeeseecseenteenees 4 22 Figure 4 18 Find Sensitivities Load Tips ssccssscssscssecssssccssccssccesscsesecessccssncesscessaseneeeeees 4 23 Figure 4 19 Find Sensitivities Tip Configuration cssccssscssccsssssssccssccssccesscessnseseesees 4 23 Figure 4 20 Find Sensitivities Load Tips error ccccccceeceesceeseceeeeeesceeseeceseceeeeeeeenseecsaeeneenaes 4 24 Figure 4 21 Find Sensitivities Choose Position cccccseceseceeseeesceesceceaeeeeeceeeeesseeesaeeneenees 4 24 Figure 4 22 Find Sensitivities Test Sensitivities is scsssszacccesssvennsensdesccaennectevesteaazseesenesenectandieg 4 25 Figure 4 23 Find Sensitivities Test Sensitivities indicating testing progress ccee 4 25 Figure 4 24 Find
126. ed maximum of 1000 uL liquid and or air capacity e Fixed 100 Coated and Uncoated maximum of 1000 uL liquid and or air capacity Note Fixed tips can pull samples into the tubing but the maximum sample volume is limited to syringe capacity refer to Section 3 4 2 Syringes Note Fixed tips can be used only with the Span 8 Pod e Septa Fluted maximum of 1000 uL liquid and or air capacity Note Septa FlutedFixed tips can pull samples into the tubing but the maximum sample volume is limited to syringe capacity refer to Section 3 4 2 Syringes gt FX Septa Fluted are only available with this instrument e Disposable 20uL LLS up to 100 uL liquid capacity up to 120 uL air capacity e Disposable 200uL LLS up to 220 uL liquid capacity up to 240 uL air capacity Note The Multichannel Pod can load disposable LLS tips but it is not liquid level sensing capable The Span 8 is the only pod capable of performing liquid level sensing therefore disposable LLS tips should be used only with a Span 8 Pod e Disposable P1000 LLS sterile and non sterile up to 1000 uL liquid and air capacity e Disposable P1000 LLS barrier up to 1000 uL liquid and air capacity Note Refer to Section 3 3 Interchangeable Tips for more information on tips available for use with the Span 8 Pod Beckman Coulter Inc Span 8 Pod 3 7 3 3 1 1 Liquid Level Sensing Settings Sensitivity settings for liquid lev
127. eeeeneeeeeeeeeeeeeeeeeaees 5 17 Figure 5 16 Manual Teaching if tips are Loaded sys sszcanstonsziesisosdsaMsvasscesecadaosensasusMeveidenlewaduerss 5 17 Figure 5 17 Manual MACHA G ccc rccntaicnceencacsaumeeesesaaekaceaseactacwnanidagesoistecanatacatoseaelayuneaveceeseentaeese 5 18 Figure 5 18 Manual Teach warning on tips descending into a microplate eeeeeeeee 5 20 Figure 5 19 Manual Teaching Teach 7 piccscvnvsnccacesbecceanricexauiateaicaussscecsamnnnseinactecilaannedewuins 5 20 Figure 5 20 Hardware LUD acc circa sd econcctonca sesaecas tuaneaenesssaasbaccuansaeenseeetacanaearqueseattansoeaimueascesaeeees 5 23 Figure 5 21 Warning to make sure gripper are ready for framing ccceeceeeeseeeteeeeeees 5 23 Figure 5 22 Pick previously framed deck position to frame the gripperS c cccccesseeeteeees 5 24 Figure 5 23 Advanced Manual Control for moving gripper during framing cee 5 25 Biomek FX and FX P Laboratory Automation Workstations User s Manual xxii List of Figures Figure 5 24 Moving pod into gripping position s sessssessesesessesstserssressesstssressessresresseeseesressee 5 25 Figure 5 25 Gripper framing position on the AccuFrame essesesessssssessseseesrsstsreseseesssseres 5 26 Figure 5 26 Correct and incorrect gripper framing alignment sseseesseseesseesesseessesseseeserssee 5 26 Figure 6 1 Confirms Manual Control is connecting eseeseeseeeseeeeceeces
128. efore removing the fourth shoulder screw take hold of the head firmly to make sure it does not fall once all screws are removed CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when installing a multichannel head WARNING Gripper fingers may bend if not taught framed properly with the AccuFrame WARNING Avoid all labware on the Biomek FX deck when removing or installing gripper fingers It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware CAUTION Use caution when removing gripper fingers Since gripper fingers mount tightly into the Multichannel Pod they pull free of the Multichannel Pod suddenly WARNING Avoid all labware on the Biomek FX deck when removing or installing grippers It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware CAUTION Do not make any changes to the pod axes limits in Hardware Setup without contacting a Beckman Coulter Service Engineer or Beckman Coulter Customer Technical Support CAUTION If the hardware configuration is not updated using Hardware Setup hardware crashes or inaccurate liquid transfer may occur CAUTION To avoid hardware crashes a new D axis limit must be established and the gripper framed in the Biomek Software after the head has been configured in Hardware Setup PEPER PEPEE ee Biomek FX and
129. el Pod To frame the grippers 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software to start the Biomek Software 2 From the Instrument menu choose Hardware Setup Hardware Setup appears Beckman Coulter Inc Framing the Biomek FX 5 23 3 Choose the desired Multichannel Pod The configuration to frame the gripper appears on the right Figure 5 20 Biomek FX Hardware Setup Change Add Remove Wane Reconnect Tools v Davee esas Accept Cancel Biomek 717 P Paaa laa Serial Number Nong Save Settings Festore Settings Delete Settings 96 Podi Head Type ES Mandrel 200 pL Head 7 Last Validation p un 14 2001 11 3 Set Validation Time 8 Pod2 Axis Limit Settings oh pia i X cm Y em Z cm DL D cm Correlate Pods 7 eviceLontroler So Teoaded Minimum 6 852 6 005 15606 58 4335 0623 er 5 Digital Devices earn 72 922 23 277 2 326 227 212 2 424 Simple SetX Set Y SetZ Set D Change Head WashPump1 Additional Pod Settings Tip And Gripper Settings Defaultworld wld Figure 5 20 Hardware Setup 4 Choose Frame Gripper 5 When a Warning appears make sure the topics that appear in the Warning are addressed and choose OK Figure 5 21 be holding anything for this operation Make sure they have been retracted Also make sure there is pod and the location that you wall frame the grippers a
130. el sensing affect the magnitude of change required for LLS tips to detect the liquid A higher sensitivity setting detects smaller changes in the capacitance detected refer to Section 6 5 6 Verifying Liquid Level Sensing 3 3 1 2 Disabling Liquid Level Sensing If LLS is disabled in the Technique governing the liquid handling procedure LLS is unavailable regardless of the type of tips attached to the probes Refer to the Biomek Software User s Manual Section 10 4 Setting Technique Values for enabling and using liquid level sensing in techniques 3 3 2 Clot Detection available only with a Biomek FX Instrument WARNING When working with blood samples observe the cautionary AN procedures as defined by your safety officer The Span 8 Pod can detect if a possible clot is on the end of a tip after an aspirate operation when conductive tips called LLS in the Tip Type Editor are attached to the probes Note Conductive tips must be attached to the probes to enable clot detection Note An LLS plate option used on a 1 x1 Passive ALP can improve clot detection capabilities refer to the ALPs User s Manual Chapter 19 1 1 1 1 x 1 Passive ALP with LLS Plate Option Conductive tips fixed disposable or septa fluted are used to determine if a clot is detected by detecting a shift in the capacitance The tip moves to a specified height within the well and then slowly moves down into the well When the tip aspirates and rises o
131. el shuttle can be positioned using the following deck positions Figure B 2 e Left side Position 1 or 2 e Right side Position 17 or 18 Mount Stacker Carousel on P1 P2 P17 or P18 Figure B 2 Stacker shuttle positions Note Do not pipette liquid into labware that is sitting on the Stacker Carousel shuttle Biomek FX and FX P Laboratory Automation Workstations User s Manual B 4 Using a Stacker Carousel To position the Stacker Carousel Position the Stacker Carousel outside the Biomek FX deck Figure B 3 Figure B 3 Stacker Carousel integration Beckman Coulter Inc Using a Stacker Carousel B 5 B 2 2 Mounting the Stacker Carousel Mounting the Stacker Carousel to the Biomek FX requires attaching an upper alignment bracket a right or left horizontal alignment bracket and a riser plate Then the Stacker Carousel is positioned and leveled Note There is a specific horizontal alignment bracket for mounting the Stacker Carousel on the right side of the Biomek FX instrument Figure B 4 and a specific horizontal alignment bracket for mounting on the left side of the instrument Figure B 5 Slot for P17 mounting Slot for P18 mounting Slot for P18 mounting Figure B 5 Left horizontal alignment bracket Biomek FX and FX P Laboratory Automation Workstations Use
132. em fluid must be degassed prior to use CAUTION Do not purge the system without mandrels installed and tubing attached to disposable or fixed tips To purge the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface WARNING Debris resulting from installing a fitting in the supply container may clog the tubing WARNING Using a dirty supply container may clog the tubing CAUTION Do not remove the cover of the Speed Pump WARNING Turn off power to the Biomek FX before connecting CAN communication cables A A A A A ZN AN A A JN A A A A A A Beckman Coulter Inc Safety Information xi WARNING Do not connect or disconnect any cable while power is applied to the Biomek FX CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when installing pin plates CAUTION Carefully hold the pin plate by the edges to avoid bending or damaging the pins CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when installing the HDR Tool Body CAUTION Carefully hold the pin plate by the edges to avoid bending or damaging the tips CAUTION Always store pins in the pin plate or pin storage case to prevent pins from getting damaged or bent CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when removing a multichannel head CAUTION B
133. ement Vector Note Hold the button down to quickly add Delta value to the Movement Vector Note Positive values move the pod to the right X and toward the front of the deck Y Positive values also move the probes up Z aspirate D and widen Span the distance between the probes Negative values move the pod to the left X and towards the back of the deck Y Negative values also move the probes down Z dispense D and narrow Span the distance between the probes Note Minimum and Maximum X Y Z and D values displayed in the Hardware Setup indicate how far the pod is able to move These values are also displayed as a tool tip when hovering over the fields in Movement Vectors Enter a value in Speed to specify the percent of the pod s maximum speed Choose Go The pod moves from its current position to a new position by the values displayed in the Movement Vector The new position is displayed in Current Position Choose Close to close Advanced Manual Control Choose Exit to close Manual Control ally Controlling the Biomek FX in Biomek Software 6 19 6 5 3 1 Setting Delta Values for the Span 8 Pod A Delta value is the amount of change applied to the Movement Vector when a button in the Vector Builder is pressed For example if the Delta value for X is 3 each time Right is clicked in the Vector Builder 3 cm is added to the X axis of the Movement Vector Note The move does not occur until Go
134. er s Manual B 28 Using a Stacker Carousel Beckman Coulter Inc B 4 3 Removing Stacker Carousels To remove Stacker Carousels 1 In Hardware Setup right click the desired device under Stacker Carousels 2 Choose Remove Device The device is removed from the installed stackers under Stacker Carousels in the left pane OR 1 Select the desired stacker under Stacker Carousel 2 Choose Remove Device from the top of Hardware Setup The device is removed the installed devices under Stacker Carousels in the left pane Using a Stacker Carousel B 29 B 5 Framing the Stacker Carousel The Stacker Carousel is framed using the AccuFrame and the Framing Tool Adaptor To frame the Stacker Carousel 1 Using the Control Pendant choose SHUTTLE IN and SHUTTLE OUT to ensure the shuttle of the Stacker Carousel is in the correct position for framing Note Refer to the Stacker Carousel User s Manual Beckman Part Number 148598 for more information on using the Control Pendant CAUTION To prevent damage to the shuttle first place the AccuFrame AN into the Framing Tool Adaptor Then place the AccuFrame with the attached adaptor on the shuttle 2 Manually place the AccuFrame into the Framing Tool Adaptor by placing the front right corner first and pushing the AccuFrame gently down into the adaptor Figure B 35 Biomek FX and FX P Laboratory Automation Workstations User s Manual B 30 Using a Stacker Carousel 3
135. er Inc 3 Turn on power to the Biomek FX instrument 4 Manually place the AccuFrame onto the ALP position that requires framing by placing the back right corner first and pushing the AccuFrame gently down onto the ALP position Note The deck is usually framed from from left to right starting at the back left position However ALPs may be framed in any order 5 Make sure that the AccuFrame is fully seated along the posts along the top and right sides of the position Note When framing a dual pod system frame both pods to each position Note Some ALPs require a framing adaptor to accommodate using AccuFrame to frame the position Make sure the correct adaptor is used for each type of ALP Framing proceeds as normal once the AccuFrame is placed on the appropriate adaptor Framing the Biomek FX 5 11 5 2 4 Framing the Position To frame the Biomek FX deck position Note Some ALPs require additional or slightly modified procedures to frame properly Consult the ALPs User s Manual for the specific ALP to frame for any special framing instructions 1 In the Biomek Software choose Instrument gt Deck Editor Deck Editor appears Figure 5 8 Deck1 Default Deck D x i B ry NewDeck Delete Deck Rename Deck OpenDeck ClearDeck Renumber Delete ALP Properties Save Cancel SpanSwWashRight SPE SPE2x1Left SPE 2x1 Right SPEHolder StackerCarousel Coords cm o fo fo Figure 5 8
136. er in place when operating or troubleshooting the laser module A A Beckman Coulter Inc To align the laser beam correctly 1 Verify that the red laser beam is scanning down across the path of the shuttle If the laser beam is not directed downward toward the shuttle turn off the Stacker Carousel power switch 2 Review Section B 3 3 Positioning Bar Code Reader before correcting the orientation of the MS710 3 Visually check to make sure the red laser beam is scanning down across the path of the shuttle If the laser beam is not directed downward toward the shuttle turn off the Stacker Carousel power switch and complete step 2 again If the laser beam is scanning down across the path of the shuttle continue to step 4 4 Make sure the laser scans down through the appropriate tunnel opening For reading labels on the narrow side of the microplate the laser should scan down through one of two slots in the top of the shuttle tunnel For reading labels on the wide side of the microplate the laser should scan down through one of the two slots in the side of the shuttle tunnel 5 Ifthe position of the MS710 needs to be adjusted loosen the screw washer assemblies that attach the bar code reader bracket Figure B 28 to the shuttle tunnel adjust the tilt of the bar code reader until it is aimed properly then tighten the screw washer assemblies Using a Stacker Carousel B 23 6 To verity correct laser alignment use th
137. es overflow to the waste container Note The 3 8 and 1 4 tubing are Tygon 2075 ultra chemical resistant All 1 8 tubing is Teflon Note All diameters refer to outer diameter dimensions Beckman Coulter Inc Span 8 Pod 3 41 3 5 3 Supply Container WARNING SPILL HAZARD CAUTION Do not kink tubing between the supply container the Span 8 pumps and the Speed Pump WARNING Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety officer gt gt amp The supply container is the source of the system fluid used by the Span 8 Pod and Speed Pump refer to Section 3 6 Speed Pump Note A Beckman Coulter Service Representative installs the Speed Pump during the initial installation of the Biomek FX instrument when one is purchased with the instrument The installation instructions in Section 3 6 Speed Pump are used when the Speed Pump is purchased and installed after initial Biomek FX instrument installation Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 42 Beckman Coulter Inc Span 8 Pod The supply container also receives system fluid overflow from the Speed Pump It is connected to the Span 8 Pod and Speed Pump with 3 8 tubing and quick disconnect fittings Note The supply container and its tubing are installed by a Beckman Coulter Service Representative during system install
138. eseeeseeeeeeees B 18 Beckman Coulter Inc List of Figures xxiii Figure B 23 Bar code reader in rear narrow side scanning position Dotto VIEW specsieescxvnaeesempaausqemimestananedaxssewvesenseexpanssenneswesomnaelaamiouuannsstixumncetss B 19 Figure B 24 Bar code reader in wide left side scanning position ccceesceeeeeeeeeeteeeeeeees B 19 Figure B 25 Bar code reader in wide left side scanning position Dott VIEW Wi esinuvcrec tea ota ecvineea ens uctesacea tineia aiae T nET ELOS STEP EATI OSANE E ACEN EENAA SAAE EENE B 20 Figure B 26 Bar code reader in front narrow side scanning position cccceeeeeeeeeeteeeeeeees B 20 Figure B 27 Bar code reader in front narrow side scanning position DOON VIEW Jeer E E T EARE E O I A E B 21 Figure B 28 Bar code reader attachment bracket 5 sccisisspdcecessisdasantacdasasannecnesetienctiontiataarbadexwates B 21 Figure B 29 Bar code reader Laser beam position over bar code label eee eeeeeeeeeeee B 23 Figure B 30 Adjust laser alignment as necessary sc acciensekcdeetianedare neice B 23 Figure B 31 Label positioning on microplate ss scsussasccessxnnesaresenvshassoesdsasersantuasdasbavinessexnphadiaass B 24 Fig re B 32 New Devices ii snanicaivsenssanieniavanniaiainsnuanancamancaeaaeameaIes B 25 Figure B 33 Adding a Stacker Carousel vais caais cxsncsasiaaetuutnsdasaatcvaatianseeupienasanssamneiaucen muna inerBlons B 26 Figure B 34 Hardware Setup showing the configuration
139. esents Table 1 1 Indicator Light Key Light Indicator State Operational Status Solid Green Idle System is functional and in a ready state It is safe to access the instrument and deck without violating the light curtain protective zone Blinking Green Pause Planned pause This may be written into a method to allow periodic access to the deck When pause is terminated the light curtain is reactivated and the method continues Solid Amber Active A method is running A violation of the light curtain will halt operation Blinking Amber Error Caused by light curtain violation or system error Halts pod and head operations immediately Software communicates the cause Beckman Coulter Inc Biomek FX Introduction 1 7 1 2 2 Bridges The Biomek FX bridges Figure 1 4 are structures that move in the X axis The bridges hold the pods and move them in the Y front to back and Z axes up and down One or two bridges are available on the Biomek FX instrument to create a single or dual pod instrument In a dual pod system the pods can work together to expand liquid handling capabilities Note The left and right bridges on a dual pod instrument are defined by viewing the instrument from the front Bridges ona single pod instrument Multichannel Pod c if q S Figure 1 4 Bridges move in the X axis and hold and move pods in the Y and Z
140. essesesseseesrsseresssrsersessrseese B 9 Figure B 10 Diagram for attaching blank to shield and shield to Biomek FX cesses B 10 Figure B 11 Attach Side shield sei ssssncotasisesisscesaaeus sesswsoandasncisadernee isk sa canton a B 11 Figure B 12 Side view with side panel attached cee ccceessecesecesceeeeeeeaeeceeeceeeeeeeeeeeeeseetees B 11 Figure B 13 Stacker Carousel final placement top view rear position right side B 12 Figure B 14 Bar code reader Wack et acavcccescascandoiccenasecacasseansnasvautexeecntoancaveecemenniamorentexweanteneea B 13 Figure B 15 Bar code reader shuttle mount access cover reMOVed ccceeeeeeseeesseeeteeeteeeees B 14 Figure B 16 Mounting bracket attachment jslvs scsusnnsasvessnedussusnsavuasnvedea suns donee tecsasseunaatuvsseduenls B 14 Figure B 17 Mounting bar code Le AU Tj sadsnsexvessgcaasvenvsansnsissansitniaineanedaennilyseuaaunbetdaeanntecannanteeine B 15 Figure B 18 Bar code reader in narrow side scanning position sssessesessssessessrssresseesessees B 16 Figure B 19 Bar code reader in narrow side scanning POSitiON c cesceceseceeeeeeeeeeeeenseeees B 17 Figure B 20 Bar code reader in wide right side scanning POSItiON ccceeeceeeseeeeeeteeeeeeeees B 17 Figure B 21 Bar code reader in wide right side scanning position DOON VIEW Ie E EE E E A E TO T B 18 Figure B 22 Bar code reader in rear narrow side scanning POSItiON cccceeeee
141. essure Level Maximum sound pressure 79 dB Maximum sound pressure at 1 meter 76 dB Fuses US 250VAC 5 amp 5x20 mm SLO BLO UL recognized CSA certified Europe 250VAC 5 amp 5x20 mm SLO BLO CENELEC approved Communications to Host RS 232 port Biomek FX and FX P Laboratory Automation Workstations User s Manual A 2 Specifications Table A 1 System Specifications Continued Beckman Coulter Inc Item Description Communications to Tip CAN Loader Communications to CAN Active ALPs Biomek controller host PC The following specifications are the minimum requirements needed for the IBM PC CPU Pentium IV 2 GHz RAM 512 MB Hard Drive 13 5 GB CD ROM Drive 24x Monitor 17 Super VGA 1024 x 768 small fonts w 16 bit color Operating System Windows XP with Service Pack la Other Software SQL Server Personal Edition B 1 Appendix B Using a Stacker Carousel B 1 Overview The Stacker Carousel Figure B 1 is integrated into the system to dispense and load labware onto the Biomek FX deck It provides expanded labware capacity and increases walk away automation in genetic analysis and drug discovery applications Each carousel contains locations for four stackers A shuttle is incorporated for transporting labware to and from the carousel Note For a more comprehensive description of the Stacker Carousel its components functionality and operations refer to
142. f cut out without aligning to inside of cut out Figure 5 26 Correct and incorrect gripper framing alignment Beckman Coulter Inc Framing the Biomek FX 5 27 9 In Frame Gripper choose OK Figure 5 24 Note The gripper is now framed for the selected pod If necessary complete the gripper framing process for the other Multichannel pod in a dual pod system 10 From Hardware Setup choose Accept Hardware Setup closes 11 From the Instrument menu choose Manual Control Manual Control appears 12 Choose Advanced Controls and select the Multichannel Pod Advanced Manual Control appears Figure 5 23 13 Choose Move Z Max Note Choosing Move Z Max raises the pod to access the AccuFrame 14 Choose Close to close Advanced Manual Control 15 Choose Exit to close Manual Control 16 Remove the AccuFrame from the ALP position 5 4 1 Testing Gripper Framing Accuracy To make sure the gripper works properly create and run a method at reduced speed around 10 to move a piece of labware from one framed deck position to another refer to Section 4 3 3 Setting Multichannel Pod Properties If problems occur repeat the gripper framing process until the gripper is working correctly Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 28 Framing the Biomek FX 5 5 Troubleshooting Perform the troubleshooting techniques provided in Table 5 1 when necessary Note In
143. fficer WARNING Observe cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials WARNING Always wear protective gloves when removing the tips CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod gt PePe EEE Note Disposable tips should be disposed of into a Span 8 Disposal ALP prior to removal of the disposal tips option Note A wrist ground strap must be attached to the instrument deck prior to installing or removing tip mandrels on the Span 8 Pod refer to Section 3 3 4 Electro Static Discharge Protection when Changing Tips on the Span 8 Pod To remove the disposable tips option 1 Unscrew the tip shuck tube counterclockwise from the bottom of the tip interface Figure 3 17 Probe Tip Interface Tip Shuck Tube Disposable Tip Mandrel Figure 3 17 Fully installed disposable tip mandrel Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 26 Span 8 Pod 2 Pull the tip shuck tube down and off the disposable tip mandrel Figure 3 18 Probe Tip Interface Disposable Tip Mandrel lt Tip Shuck Tube Figure 3 18 Removing a tip shuck tube from a disposable tip mandrel 3 Unscrew the collar countercl
144. ft position for appropriate teaching instructions refer to Section 5 2 4 1 Selecting Appropriate Teaching Instructions 10 Ifthe coordinates displayed appear reasonable choose OK Position Properties appears again and the position is framed for the selected pod 11 Choose OK to close Position Properties 12 For framing additional positions move the AccuFrame to the next position to frame refer to Section 5 2 3 Installing AccuFrame and repeat steps 3 through 11 Note When framing positions using the Span 8 Pod the framing shaft must be moved to probe 7 to frame positions in the front row of the deck refer to Section 5 2 2 3 Attaching the Framing Shaft to the Span S Pod 13 Choose Save to save framing information for all positions and close the Deck Editor Figure 5 8 Note Choosing Cancel loses all changes to the deck including framing information since the Deck Editor was opened Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 144 Framing the Biomek FX 14 Remove the framing fixture from the head of the Multichannel Pod OR Remove the framing shaft from the probe on the Span 8 Pod 5 2 4 1 Selecting Appropriate Teaching Instructions In Teaching Instructions Figure 5 11 the entire deck a deck position or an ALP can be shifted by the teaching process Determine what should be shifted using the following information taught Shift deck shifts all ALPs and position
145. g the syringe To set the valves on the probes 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Span 8 Pod appears 4 Choose Set Valve States Valve Setter appears Figure 6 15 Valve Settings x Valves None Vi M2 M3 M4 M5 ME M M8 Input Output Bypass Done Figure 6 15 Valve Settings 5 Select the valves by placing a checkmark next to the valve s to be set Note Choosing All selects all of the valves choosing None selects none 6 Choose Input to open the valve and allow system fluid to flow to and from the syringe with the supply container as the source destination of the fluid OR Choose Output to open the valve and allow system fluid to flow to and from the syringe with the tip as the source destination of the fluid OR Choose Bypass to open the valve and allow system fluid to flow through the valve without activating bypassing the syringe 7 Choose Done after the valves have been set as desired Valve Setter closes 8 Choose Close to close Advanced Manual Control 9 Choose Exit to close Manual Control Biomek FX and FX P Laboratory Automation Workstations User s Manual Manually Controlling the Biomek FX in Biomek 6 5 6 Verifying Liquid Level Sensing It may be useful to verify the operation of liquid level sensing Use LLS Z Mo
146. g the Z AXES icant awh GPG EREN kE ead eed EEEN 4 21 4 4 5 Performing Find LLS Sensitivities nanan nananana 4 21 4 4 6 Performing Clot Detection Sensitivities nnana uaan anaana 4 26 4 4 7 Setting Span 8 Pod Properties naunau nananana 4 31 4 4 8 Enabling Purge Settings lt sc 455 ches dene eede anrr 4 33 4 5 Saving Restoring and Deleting Settings 00 00 c eee eee 4 34 4 5 1 Saving Pod Settings yaeeh datas eens senda desea anes 4 34 4 5 2 Restoring Pod Settings 06 cc cece cece cece ee ne ee eees 4 35 4 5 3 Deleting Pod Settings a5 cas iaveere steko ladskaw yards tues yas 4 36 Biomek FX and FX P Laboratory Automation Workstations User s Manual xviii Table of Contents 5 Framing the Biomek FX 5 1 OVEIVIEW reae sc ea hea a aa en ea e eaa eaae eee 5 1 5 1 1 Precision When Teaching Two Pods 000 cee eee 5 1 5 2 Framing Deck Positions on the Biomek FX Using AccuFrame 5 2 5 2 1 Homing All Axes of the POdSi 4s0se0 0 dees shone y eis eeeeaess 5 3 5 2 2 Attaching the Framing Fixture to the Pod 00 5 4 5 2 3 Installing ACCUPTAMNG sccicreiiriecrrii rid rur EKEN EE EESTI 5 10 5 2 4 Framing the Position nnana ad een cox oe ORS Ree Re eS 5 11 5 3 Manually Framing Deck Positions on the Biomek FX 5 15 5 4 Framing the Gripper on a Multichannel Pod 0 000005 5 22 5 4 1 Testing Gripper Framing
147. g the supply container Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 44 Span 8 Pod PERE BE Beckman Coulter Inc 3 5 4 Waste Container WARNING SPILL HAZARD CAUTION Do not kink tubing between the waste container the Span 8 Pod pumps and the Span 8 Tip Wash ALP WARNING Use an appropriately contained environment when using hazardous materials WARNING Observe cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials WARNING Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety officer The waste container Figure 3 31 is an off deck disposal site for fluid from the Span 8 pumps and the Span 8 Tip Wash ALP refer to the ALPs User s Manual Chapter 18 Span 8 Tip Wash ALP It is connected to the Span 8 pumps and the Span 8 Tip Wash ALP with 3 8 tubing and quick disconnect fittings Note Separate waste containers can be used for each Span 8 Tip Wash ALP on the deck if it is undesirable to mix the contaminates washed off the tips Note The waste container and its tubing are installed by a Beckman Coulter Service Engineer during system installation however when the container is full it must be replaced Span 8 Pod 3 45 Span 8 Tip Wash ALP Waste Connection Used wash fluid from the Span 8 Tip Wash ALP terminates in the
148. g will not function i CAUTION Do not choose Cancel if testing is stopped before the probe and Find Sensitivities must be repeated Note It takes several minutes to complete the testing When testing is completed choose Next Find Sensitivities Update Settings appears indicating the probe sensitivities have been completed Figure 4 24 Note If tips have been configured to unload they will unload now Updating Probe Sensitivities Completed Probe 1 3750 Updated Probe 2 3830 Updated Probe 3 3860 Updated Probe 4 3900 Updated Probe 5 3900 Updated Probe 6 3650 Updated Probe 7 3470 Updated Probe 8 3650 Updated Update Settings Figure 4 24 Find Sensitivities Update Settings 11 Choose Finish Find Sensitivities closes 12 Choose Accept Hardware Setup closes 4 4 6 Performing Clot Detection Sensitivities available only with a Biomek FX Instrument Performing Find Clot Detection Sensitivities is necessary to test each probe on an individual system to ensure the clot detection sensing capability is calibrated This procedure adjusts the system to the individual clot detection sensitivity settings on each probe Although Find Clot Detection Sensitivities is performed during initial setup by a Beckman Coulter Service Engineer the procedure must be repeated when e Changing from disposable to fixed tips e Changing from fixed to disposable tips e Difficult
149. gure 3 27 Removing syringes unscrewing and pull down on the syringe Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 36 Span 8 Pod 5 Gently pull the plunger out of the glass casing Figure 3 28 4 Glass Casing lt _ Plunger Figure 3 28 Removing syringes pulling the syringe out of the glass casing 6 Return the syringe components plunger and glass casing to their original packaging material Note Syringes are configured in two sets of four Each set of four syringes may be configured with different size syringes 3 4 2 5 Syringe Cutoff Velocities The speed at which fluid is dispensed per second prior to the dispense action abruptly stopping is called the Cutoff Velocity If the Cutoff Velocity in the technique or liquid type governing the pipetting operation is too slow droplets can be left at the end of tips after a dispense pipetting operation Accurately setting the Cutoff Velocity causes the liquid to be ejected at a speed sufficient to prevent droplets from forming on the end of a tip Note Refer to the Biomek Software User s Manual Section 10 4 8 1 Syringe Cutoff Velocities FX NX S8 only and the Biomek Software User s Manual Section 9 2 2 Changing the Cutoff Velocity for Span 8 Pods in Liquid Type Editor FX NX S8 only Beckman Coulter Inc Span 8 Pod 3 37 The minimum and maximum Cutoff Velocity for the
150. gure 4 29 Find Clot Detection Sensitivities Test Sensitivities Find Clot Detection Sensitivities Start 5 Testing Progress Load Tips Determining maximum sensitivities for each probe Test Sensitivities Update Settings Figure 4 30 Find Clot Detection Sensitivities Test Sensitivities testing progress Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 31 sensitivities have been completed clot detection will not function and Find Clot Detection Sensitivities must be repeated It takes nearly 30 minutes to complete the testing f Do not choose Cancel if testing is stopped before the probe 11 When testing is completed choose Next Find Clot Detection Sensitivities Update Settings appears indicating the probe sensitivities have been completed Figure 4 31 Find Clot Detection Sensitivities Start Updating Probe Sensitivities Completed Load Tips Probe 1 4600 Updated Probe 2 4700 Updated Probe 3 4600 Updated Probe 4 4600 Updated Probe 5 4800 Updated Probe 6 4700 Updated Probe 7 4600 Updated Probe 8 4100 Updated Update Settings Figure 4 31 Find Clot Detection Sensitivities Update Settings Note If tips have been configured to unload they will unload now 12 Choose Finish Find Clot Detection Sensitivities closes 13 Choose Accept Hardware Setup closes 4 4 7 Setting Span 8 Pod Properties Most of the propertie
151. gured Load Ti ps will Probe 5 Disposable tip No tip configured not be di splayed Probe 6 Disposable tip No tip configured H Probe 7 Disposable tip No tip configured here if all probes Probe 8 are configured with fixed tips Ci Le Figure 4 16 Find Sensitivities Start Disposable tip No tip configured This wizard assists with determining the maximum sensitivity for each probe This will help minimize errrors created by receiving a false Load Tips detection Choos All of the probes will need to have tips loaded Any probe Test S a configured to use disposable tips will have to be loaded with a o gt a conductive tip Update senings The current tip configuration is Probe 1 Disposable tip No tip configured no tip detected Probe 2 Disposable tip No tip configured no tip detected Probe 3 Disposable tip No tip configured no tip detected Probe 4 Disposable tip No tip configured no tip detected Probe 5 Disposable tip No tip configured no tip detected Probe 6 Disposable tip No tip configured no tip detected Probe 7 Disposable tip No tip configured no tip detected Probe 8 Disposable tip No tip configured no tip detected Figure 4 17 Find Sensitivities Start tip detection Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 23 3 Choose Next Find Sensitivities Load Tips appears Figure 4 18 Note If tips are
152. h those tips varies for each head as described in Table 2 2 Note The HDR Tool Body uses pin plates to perform low volume liquid transfers by liquid adhesion refer to Section 2 3 1 Interchangeable Pin Plates for the HDR Tool Body Table 2 2 Tip compatibility and max volumes on Multichannel Heads Som Son 2 Si a f N se a a 96 Channel 20 uL Head 55 uL 20 ph SSuL 50 uL 96 Channel 200 uL Head 120 uL 20 uL 225 uL 125 uL 384 Channel 30 uL Head 35 ub 30 uL Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 6 Multichannel Pod Beckman Coulter Inc 2 3 1 Interchangeable Pin Plates for the HDR Tool Body An interchangeable pin plate is attached to the mount plate on the bottom of the HDR Tool Body Pin plates are available in 96 pin and 384 pin capacities and may have any of the pin types presented in Table 2 3 Table 2 3 Pin Types Pin Type Description 0 045 Post A cylindrical pin with a 0 045 diameter SE Can access 96 and 384 well microplates 0 015 Post A cylindrical pin with a 0 015 diameter Can access 96 384 and 1536 well microplates 0 015 Nail A cylindrical pin with a 0 015 conical tip Pi Can access 96 and 384 well microplates 2 3 1 1 Installing Pin Plates To install a pin plate on the HDR Tool Body Note When a pin plate is changed the
153. hen removing or installing gripper fingers It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware CAUTION Use caution when removing gripper fingers Since gripper fingers mount tightly into the Multichannel Pod they pull free of the Multichannel Pod suddenly gt e To remove gripper finger 1 Move the Multichannel Pod to the maximum Y and Z axis limits refer to Section 6 4 Using Advanced Manual Control with the Multichannel Pod 2 Grasp both sides of the gripper Figure 2 16 3 Apply force in a downward direction to pull the gripper from the head Note Since gripper figners mount tightly into the Multichannel Pod they pull free of the Multichannel Pod suddenly Front gripper assembly Gripper shaft Gripper gt j finger Gripper pads Front of gripper Figure 2 16 Removable front gripper assembly Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 24 Multichannel Pod 2 6 1 2 Installing Gripper Fingers WARNING Avoid all labware on the Biomek FX deck when removing or AN installing grippers It is recommended that all labware in the vicinity of the pod be moved or removed to avoid spills and contact with labware To install gripper fingers 1 Move the Multichannel Pod to the maximum Y and Z axis limits refer to Section 6 4 Using A
154. hoose Help gt About from Biomek Software Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 11 When the gripper has a plate sensor and Enable Plate Sensor Figure 4 6 is checked in Hardware Setup and for the specific labware in the Labware Type Editor labware can be sensed when the gripper is squeezed Biomek Hardware Setup Reconnect A Home All Axes Gb Add Device m Remove Device J Accept gt lt Cancel po None Serial Number None Save Settings Restore Settings Delete Settings 96 Podi Head Type 36 Mandrel 200 pL Head gt Last Validation Not Specified Set Validation Time 8 Pod2 Axis Limit Settings fF Devices om Correlate Pods g7 DeviceControllerO EF ShakerALPo ean 69 5 15 58 434 fast Sats A ShakerALP1 PEST 725 2 229 2404 g7 SpeedPumpO g7 SpeedPump1 oF StirrerALPO g7 StirrerALP1 Additional Pod Settings g7 TiploaderO 6 Tiploader1 Tip And Gripper Settings Ey Digital Devices Gripper Settings E Simulator cm Y cm Z cm D cm E Camera Gripper Offset j 43764 fas2 6 5532 os Materials n Stacker Carousels Gripper Extend Time 0 55 s J Enable Plate Sensor 4 Enable Plate Sensor Gripper Retract Time 0 56 s M Tip Load Setting Tip Unload Settings E po Tip Settle Time e s Talisca Unload Speed 20 i iti jo isle eel ii Final D Position po cm Set Set S Change Head
155. humbscrew up to the base of the syringe Figure 3 25 8 Tighten the thumbscrew at the base of the syringe by turning the thumbscrew to the right until finger tight lt Pump Valve Syringe Base of the syringe Thumbscrew Figure 3 25 Installing syringes push the thumbscrew up Note Four syringes are configured at a time syringes for probes 1 through 4 and syringes for probes 5 through 8 Probes 1 through 4 may be configured with one size of syringe and probes 5 through 8 may be configured with another size of syringe Beckman Coulter Inc Span 8 Pod 3 35 3 4 2 4 Removing and Disassembling Syringes To remove and disassemble syringes 1 Loosen the thumbscrew at the base of the syringe by turning the thumbscrew to the left 2 Pull the thumbscrew down away from the syringe Figure 3 26 Pump Valve Syringe Base of the Syringe Thumbscrew Figure 3 26 Removing syringes loosen the thumbscrew and pull down 3 Unscrew the top of the syringe from the valve by turning the syringe to the left 4 Gently pull the syringe down from the valve Figure 3 27 Pump Valve Top of the Syringe Syringe Base of the Syringe Thumbscrew Fi
156. i Index Numerics 384 Channel 30uL Head description 2 5 96 Channel 200uL Head description 2 5 A Absolute Move using in Advanced Manual Control 6 13 6 20 AccuFrame defined 5 2 installing the 5 10 troubleshooting 5 28 using the 5 10 active ALPs defined 1 8 Advanced Manual Control accessing 6 6 defined 6 1 selection areas for Multichannel Pod 6 8 selection areas for the Span 8 Pod 6 16 verifying liquid level sensing 6 22 ALPs active defined 1 8 defined 1 8 overview 1 8 passive defined 1 8 B Bar Code Reader aligning laser beam B 22 applying power to B 22 attachment bracket B 21 integrating with Stacker Carousel B 13 positioning labels on microplates B 24 positioning on Stacker Carousel B 16 Biomek FX described 1 1 main components 1 3 ALPs 1 8 bridge 1 7 canopy 1 11 deck 1 8 Device Controller 1 8 heads 1 7 light curtain 1 10 pods 1 7 protective side safety shields 1 11 tower 1 4 bridge defined 1 7 C canopy defined 1 11 clot detection defined 3 7 how to disable 3 8 performing Find Clot Detection Biomek FX and FX P Laboratory Automation Workstations User s Manual xxviii Index Sensitivities 4 26 sensitivity settings 3 7 tips supported 3 7 control modes for the Multichannel Pod 2 4 for the Span 8 Pod 3 4 overview 1 2 Correlating the Pods in Hardware Setup 4 8 Correlating the Z Axes in Hardware Setup 4 21 cutoff velocity defined 3 30 equation to determine 3 36 maximum 3 36 minimum
157. ienced using liquid level sensing To perform Find LLS Sensitivities 1 In Hardware Setup select the appropriate Span 8 Pod from the left pane to access the configuration area in the right pane Figure 4 14 Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 Biomek FX and FX P Laboratory Automation Workstations User s Manual Configuring the Biomek FX in Hardware Setup 2 Choose Find LLS Sensitivities Find Sensitivities opens with Start Figure 4 16 After a few seconds the tips are detected and Figure 4 17 appears Note On the left side of Find Sensitivities a list of steps required to complete the procedure is displayed As the steps of Find Sensitivities are accessed they are highlighted on the left Note A Span 8 Pod that is configured with all fixed tips will not display Load Tips on the left side of Find Sensitivities This wizard assists with determining the maximum sensitivity for each probe This will help minimize errors created by receiving a false ad Tips detection Chions All of the probes will need to have tips loaded Any probe Tes configured to use disposable tips will have to be loaded with a oe sa conductive tip Update Settings The current tip configuration is Probe 1 Disposable tip No tip configured Probe 2 Disposable tip No tip configured Probe 3 Disposable tip No tip configured r Probe 4 Disposable tip No tip confi
158. ientation Mount bracket Bar code reader laser beam Figure B 12 Side view with side panel attached Biomek FX and FX P Laboratory Automation Workstations User s Manual B 12 Using a Stacker Carousel 5 Connect the Stacker Carousel Figure B 13 Note To connect the Stacker Carousel connect the supplied serial cable from the side panel of the Stacker Carousel to a PC Controller s communication port If more than two communication ports are required install the extra serial ports in the PC at this time J e N _ 4 Figure B 13 Stacker Carousel final placement top view rear position right side B 2 4 Operating the Stacker Carousel To operate the Stacker Carousel refer to the Stacker Carousel User s Manual supplied with the Stacker Carousel Note Do not pipette liquid into labware that is on the Stacker Carousel shuttle Note The Stacker Carousel shuttle must be framed using Teach do not use Auto Teach or Manual Teach Refer to the Stacker Carousel User s Manual for more information on framing the Stacker Carousel B 2 5 Preventive Maintenance and Troubleshooting Refer to the Stacker Carousel User s Manual for information on preventive maintenance and troubleshooting Beckman Coulter Inc Using a Stacker Carousel B 13 B 3 Integrating the Bar Code Reader A bar code reader may be mounted to the Stacker Carousel to scan any on
159. ies such as false trips or failed senses are experienced using clot detection Beckman Coulter Inc Configuring the Biomek FX in Hardware Setup 4 27 To perform Find CD Sensitivities 1 In Hardware Setup select the Span 8 Pod from the left pane to access the configuration area in the right pane 2 Choose Find CD Sensitivities Find Clot Detection Sensitivities opens with Start Figure 4 25 After a few seconds the tips are detected and Figure 4 26 appears Reconnect Hf Home All Axes Sp Add Device s Re ce J Accept gt lt Cancel El B Biomek NX SN None Probe Configuration fj AccuFrame Click to select which probes to configure 8 pod Last Validation Not Validated Set Validation Time EME Devices gF DeviceControllerd Axis Limit Settings 7 PurgePumpo X cm Y cm Z cm D u Span cm s pe l l l Minimum 2 11 5 5 15 938 fo 6 3 oF StirrerALPO Maximum 102 79 40 fiz 1381 1000 14 7 oF StirrerALP1 Probes 1 4 use Set Set Y i pe Data Devices Fea z 4100 7 tips psa e i imulator 5 camera fim syringes Correlate Pads Correlate Z Axes Find LLS Sensitivities OF Materials Disabled Probes f Stacker Carousels l 2 3j 4 5 7 Pump fPurgePumpo gt Find CD Sensitivities Y Additional Pod Settings Save Settings Restore Settings Delete Settings Y Gripper Settings BiomekNX Span_ Figure 4 25 Hardware Setup Find CD Sensitivitie
160. ilitate the proper collection treatment recovery recycling and safe disposal of the device This symbol indicates that a potential hazard to personal safety exists from a laser source When this symbol is displayed in this manual pay special attention to the specific safety information associated with the symbol Laser Specifications Laser Type Class II Laser Diode Maximum Output 11 mW Wavelength 670 Safety Information vii Chemical and Biological Safety Normal operation of the Biomek FX may involve the use of materials that are toxic flammable or otherwise biologically harmful When using such materials observe the following precautions e Handle infectious samples according to good laboratory procedures and methods to prevent the spread of disease e Observe all cautionary information printed on the original solutions containers prior to their use e Dispose of all waste solutions according to your facility s waste disposal procedures e Operate the Biomek FX in accordance with the instructions outlined in this manual and take all the necessary precautions when using pathological toxic or radioactive materials e Splashing of liquids may occur therefore take appropriate safety precautions such as using safety glasses and wearing protective clothing when working with potentially hazardous liquids e Use an appropriately contained environment when using hazardous materials e Observe the appropriat
161. imizes drainage from the Span 8 Tip Wasp ALP and the Span 8 pumps Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 46 Span 8 Pod 6 Screw the cap on the waste container by turning the cap clockwise until hand tight 7 Reattach the pump and Span 8 Tip Wash ALP waste connections by pressing the quick disconnect fittings down onto the fittings on the waste container cap until the connections snap into place The waste container has been replaced Quick Disconnect Fittings Pump Waste Span 8 Tip Wash ALP Connection Waste Connection Waste from the Span 8 Used wash fluid from the pump assembly overflow Span 8 Tip Wash ALP terminates in the waste terminates in the waste container container Cap Figure 3 32 Replacing the waste container 3 5 5 Preparing the Liquid System Air must be removed from the liquid system to maximize the pipetting performance of the Span 8 Pod Air removal is accomplished by e Degassing the system fluid refer to Section 3 5 5 1 Degassing the System Fluid e Priming the system filling all tubing with system fluid refer to Section 3 5 5 2 Priming the System with System Fluid e Purging the tubing of air by passing a high volume of system fluid through the tubing refer to Section 3 5 5 3 Purging the Tubing and Syringes of Air 3 5 5 1 Degassing the System Fluid f CAUTION The system fluid must
162. ing the Gripper Section 6 4 5 the Multichannel Pod and the Span 8 however the dialogs are different and the buttons for performing these activities may be placed in different areas Vector Builder Builds a movement vector controls the direction and distance the pod moves These choices change the values in the Movement Vector displayed Delta Controls the amount of change applied to the Movement Vector when the Vector Builder is used r aac Left ri lt gt UnSgueeze Dispense D Units Sets the D axis units Choose cm to set squeeze units and or uL to set aspirate units x 1431 cm Y 986 cm Z 775 cm D a cm x jo cm Y jo cm Z jo cm D jo cm IV AutoClear 7 Clear Speed 100 Z Go Stap Current Position Displays the current position of the selected pod below Home D Advanced Manual Control Pod Multichannel Moves D axis to M Vector Builder the home position Honezoee Home Z XY Extend Gripper Moves Z and then X and Y Retract Gripper Fwd ae axes to home positions Bove Akak gt j Absolute Move Move Z Max Squeeze Aspirate Moves pod to Current Position highest configured height M Movement Vector Absolute Move Builds a vector to an absolute coordinate from the current coordinate Movement Vector Indicates the amount th
163. ing the pins 3 Slide the pin plate up between the gripper to the HDR Tool Body such that the holes in the pin plate line up with the locating pins on the HDR Tool Body 4 While holding the pin plate in position tighten the two Phillips head captive screws to attach the pin plate to the HDR Tool Body Figure 2 8 2 Figure 2 4 Fastening the pin plate to the HDR Tool Body 2 3 1 2 Removing Pin Plates To remove a pin plate from the HDR Tool Body CAUTION To prevent damage due to electrical static discharge ESD UN wear a wrist ground strap when installing the HDR Tool Body Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek FX instrument 1 Using Manual Control lower the pod along the D axis 0 9 cm from the maximum height and extend the grippers 2 Turn off power to the Biomek FX instrument CAUTION Carefully hold the pin plate by the edges to avoid bending or UN damaging the tips 3 While holding the pin plate loosen the two Phillips head captive screws to free the pin plate from the HDR Tool Body Figure 2 8 4 Remove the pin plate by sliding it down between the grippers 5 Store pin plate according to instructions in Section 2 3 1 4 Storing HDR Pins Beckman Coulter Inc Multichannel Pod 2 9 2 3 1 3 Replacing Bent or Damaged Pins To replace damaged o
164. inin ia e RAE i EEE EEEE DiE 3 29 Figure 3 23 Installing syringes assembling the syringe ss seeseseseessesesssressessresressessssees 3 31 Figure 3 24 Installing syringes push the syringe up into the valve nd SCLEW it in opsscineds s ndsangs secu sannesaseadndenseaagsaninaninancnaadsadinastntanseaniaaeneaes 3 32 Figure 3 25 Installing syringes push the thumbscrew up sssesssssssessssssesesstsresesseseessssesses 3 33 Figure 3 26 Removing syringes loosen the thumbscrew and pull down seers 3 34 Figure 3 27 Removing syringes unscrewing and pull down on the syringe eee 3 34 Figure 3 28 Removing syringes pulling the syringe out of the glass casing 06 3 35 Fig re 3 29 S pply container ssessscssnnsn nn nian E acne ei R eaten 3 41 Figure 3 30 Replacing the supply container scsisscnssevesaasessnediacensnniunesassinssasssa stu saieunsoeaustontiuets 3 42 Figure 3 31 Waste container pues asseacrasaseseneccensaenseanneeaacesuasausesaaveceaimensancdeeatbanapennstaseasbaienentneess 3 44 Figure 3 32 Replacing the waste comtaimer is lt sicsassisaecasassatecsearssestanssavaass caceaass snuvdevavicousavaeesends 3 45 Figure 3 33 Speed Pump front view ss ssssessseessesessseessesersseesseserssressessrssresressessrssressessessees 3 47 Figure 3 34 Speed Pump back View cccscscaccsaicecessscscecvsuss cneceaseowiesacansaeaserveacusaareatoseistaccnameneen 3 48 Figure 3 35 Tubi
165. into the microplate Note Manual Teach should be used only to frame the HDR Tool Body with a 384 pin plate installed Automated framing is the recommended mode of framing when using a 96 pin plate Figure 5 4 HDR Framing Fixture To frame the HDR Tool Body CAUTION To prevent damage due to electrical static discharge ESD AN wear a wrist ground strap when installing the HDR Framing Fixture Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek FX instrument 1 Using Manual Control lower the pod along the D axis 0 9 cm from the maximum height and extend the grippers 2 Turn off power to the Biomek FX instrument 3 Slide the HDR Framing Fixture up between the gripper fingers to the HDR Tool Body such that the holes in the HDR Framing Fixture line up with the locating pins on the HDR Tool Body Note Ifa pin plate is already installed on the HDR Tool Body remove the pin plate before installing the HDR Framing Fixture refer to Section 2 3 1 2 Removing Pin Plates Beckman Coulter Inc Framing the Biomek FX 5 7 4 Tighten the two thumb screws to fasten the HDR Framing Fixture to the HDR Tool Body Figure 2 8 Thumbscrews Figure 5 5 Fastening HDR Framing Fixture to the HDR Tool Body 5 Install AccuFrame in the position to frame refer to Section 5 2 3
166. ions on the Biomek FX can also be framed by choosing Manual Teach on Position Properties Manual Teach involves loading tips and visually aligning them with the wells on a piece of labware refer to Section 5 3 Manually Framing Deck Positions on the Biomek FX Manual Teach is useful if specialized or very high density labware is used or when Auto Teach yields unsatisfactory results Some ALPs such as the Span 8 Tip Wash ALP and the Positive Position ALP must be framed manually 5 2 1 Homing All Axes of the Pods CAUTION Before selecting OK to home all axes the following UN conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the gripper on the Multichannel Pod is retracted Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure that the two arms are not near each other at either end of the rail Prior to framing the Biomek FX deck with either a Multichannel Pod or a Span 8 Pod all axes must be homed Homing the pods gives Biomek FX a point of reference from which to make subsequent moves For a single pod system home position is left back For a dual pod system home position for the first left pod is left back and for the second right pod is right back Note Home the pods each time the Biomek FX instrument is powered on Whe
167. is selected in Movement Vector of Advanced Manual Control To set Delta values 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Span 8 Pod appears Figure 6 12 4 Enter a value in X Y Z D and Span to set the Delta value for each axis 5 Choose Close to close Advanced Manual Control 6 Choose Exit to close Manual Control 6 5 3 2 Understanding and Using the Vector Builder for the Span 8 Pod The use of Advanced Manual Control for the Span 8 Pod centers around building and applying movement vectors using the Vector Builder A movement vector indicates the magnitude and direction of motion applied to the pod or probes Use the Vector Builder buttons Figure 6 13 to add positive or negative values to the Movement Vector for the pod Each time a Vector Builder button is pressed the Delta value for that axis is added or subtracted from the appropriate Movement Vector for the pod With the point of reference at the front and center of the unit positive and negative values for the pod are as follows gt Right Aspirate Dispense irl ry pa coo0 Joong Widen Narrow Figure 6 13 Vector Builder for the Span 8 Pod e Positive value X right motion e Negative value X left motion of the pod of the pod e Positive value Y forward e Negative value Y back motion motion of the pod of the po
168. ith the HDR Make sure pins are clean and replace any Pin Tool Body are not accurate bent pins Contact a Beckman Coulter Service Engineer Front gripper shaft is bent Replace the front gripper fingers using Front Removeable Rod Assembly Beckman Coulter Part Number 394062 Refer to Section 2 6 1 Replacing Gripper Fingers for instructions Front gripper pads look worn Rear gripper shaft is bent Replace the rear gripper fingers using Rear Removeable Rod Assembly Beckman Coulter Part Number 394063 Refer to Section 2 6 1 Replacing Gripper Fingers for instructions Rear gripper pads look worn Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 22 Multichannel Pod 2 6 1 Replacing Gripper Fingers Gripper fingers are easily replaced by pulling the damaged gripper fingers out of the head and pushing the replacement fingers into the head Note Not all Multichannel Pods are equipped with the replaceable gripper If the Multichannel Pod does not have the replaceable gripper contact a Beckman Coulter Service Engineer for assistance Pod front a b Pod rear g mre Front removeable Rear removeable gripper assembly gripper assembly Figure 2 15 Installed replaceable gripper fingers Beckman Coulter Inc Multichannel Pod 2 23 2 6 1 1 Removing Gripper Fingers WARNING Avoid all labware on the Biomek FX deck w
169. ivity as follows Use Manual Control in the software and insert the material no more than 1 past and approximately 21 above the light curtain panel Make sure the solid amber indicator light changes to blinking amber The diffuse reflective perimeter light curtain along the front edge of the Biomek FX instrument is a standard component however light curtains can also be installed along both sides of the Biomek FX instrument Note Either side light curtains or side safety shields must be installed by a Beckman Coulter Service Representative when the Biomek FX instrument is installed The light curtain projects a diffused array of infrared light rather than a vertical laser like beam Figure 1 5 When a part of the human body or an object larger than approximately 1 in diameter such as labware and large cables penetrates this protective zone the instrument shuts down immediately stopping all pod and head operations Some ALP operations such as shaking or stirring continue ALPs respond to a violation according to safety and operational requirements specific to each ALP For example a refilling reservoir may continue to operate if user safety is not compromised ALPs that operate with a motion that could present a danger to the operator go to a safe state when the light curtain is violated Note When active ALPs or optional devices are operating and the light curtain is violated an error message may not appear until after the AL
170. lar is securely tightened to the tip interface Leaks are occurring around Make sure the disposable tip mandrel disposable tips is inserted securely into the tubing Cut off approximately 2 of tubing off the end to ensure a tight fit Make sure the collar is securely tightened to the tip interface Disposable tips are not mounting Make sure the tip shuck tube is correctly securely tightened to the tip interface Disposable tips are not shucking Make sure the collar is securely tightened to the tip interface If the collar is loose turn the collar clockwise until a tight fit is achieved Refer to Section 3 3 6 1 Installing Disposable Tip Mandrels for more information Biomek FX and FX P Laboratory Automation Workstations User s Manual Table 3 8 Span 8 Pod Troubleshooting Continued If Liquid level sensing is not working Then Make sure LLS tips are being used refer to Section 3 3 Interchangeable Tips Make sure LLS tips are correctly mounted to the probes refer to Section 3 3 5 Fixed Tips and 3 3 6 Disposable Tip Mandrels Make sure LLS is enabled in the Technique governing the method Refer to the Biomek Software User s Manual Chapter 10 Understanding and Creating Techniques and the Biomek Software User s Manual Chapter 9 Understanding and Creating Liquid Types Contact a Beckman Coulter Service Engineer Aspirate and dispense actions are not
171. lities Beckman Coulter Inc Biomek FX Introduction 1 3 1 2 System Components The system components described below correspond to the components shown in Figure 1 1 Span 8 Pods and Heads Section 1 2 3 Multichannel Pods and Heads Section 1 2 3 Bridges Section 1 2 2 i A gt ALPs Section 1 2 4 1 Figure 1 1 Biomek FX main components Biomek FX and FX P Laboratory Automation Workstations User s Manual 1 4 Biomek FX Introduction 1 2 1 Towers The Biomek FX towers form the rear vertical and horizontal uprights of the base unit along which the bridges travel in the X axis The links for master control of the Biomek FX plus utility hook ups and ALP connections are on the towers Built into the towers are green and amber indicator lights that keep users aware of the current operational status of Biomek FX instrument X axis linear rail Left tower Inside tower connections see Figure 1 3 for close up X axis travel plate Outside left tower connections see Figure 1 3 for close up Beckman Coulter Inc Figure 1 2 Main components and connections of the Biomek FX towers Biomek FX Introduction 1 5 The towers house the following main components Figure 1 2
172. ly container in the same location the original supply container occupied 3 43 Note The supply container is positioned at installation to minimize tubing length and to keep kinks and bends out of the tubing and to reduce uphill slopes as much as possible Positioning the new supply container in the same location ensures proper alignment of the tubing and reduces pipetting errors due to air in the tubing Lower the cap so that the internal supply tubing is inside the new supply container Screw the cap on the supply container by turning the cap clockwise until hand tight Reattach the supply and overflow connections by pressing the quick disconnect fittings down onto the fittings on the supply container cap until the connections snap into place The supply container has been replaced Overflow Connection Overflow from the Speed Pump is directed back into the supply container This connection is used only when a Speed Pump is installed Fittings Quick Disconnect Supply Quick Disconnect Fittings Pressing on the tabs on the quick disconnect fittings releases the fittings Nea 3 8 Tubing NUL Internal tubing is attached to the supply connection not the overflow connection Supply Connection The supply line runs from the supply container to the Span 8 pumps or to the Speed Pump Cap Figure 3 30 Replacin
173. n attempting to use the pod error messages result until the pod is homed Note While it is necessary to home the pods after the Biomek FX instrument is powered on it is not necessary to home the pods each time the host computer is turned on or the software is accessed Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 4 Framing the Biomek FX f CAUTION Always ensure that the gripper is retracted before homing To home the pods 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software to open the Biomek Software 2 From the Instrument menu choose Home All Axes Warning appears Figure 5 2 Note Home All Axes may also be accessed by choosing Instrument gt Manual Control gt Home All Axes or Hardware Setup gt Home All Axes Note Additional Warnings and Information may appear depending on the instrument configuration Note Choosing Home All Axes homes all of the axes for all pods Warning a x CAUTION Before selecting OK to home all axes the following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the grippers on the Multichannel Pod are retracted Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure that the two arms are not near each other at either end of the rail
174. n contract tort including negligence breach of warranty strict liability or otherwise and including but not limited to damages resulting from loss of data loss of anticipated profits or any special indirect incidental or consequential damages In no event shall Beckman Coulter Inc s liability to the user exceed the amount paid by the user to Beckman Coulter Inc hereunder The user assumes full responsibility for the results obtained from the use of this system and related documentation and for application of such results Beckman Coulter SAGIAN Operations Indianapolis Indiana eestannleto SILAS is a trademark and Biomek SAMI and ORCA are registered trademarks of Beckman Coulter Inc Microsoft and MS DOS are registered trademarks and Windows is a trademark of Microsoft Corporation All other trademarks appearing in this manual are owned by their respective companies Copyright 2010 by Beckman Coulter Inc All rights reserved No part of this publication may be reproduced distributed or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise or stored in a database or retrieval system without the prior written permission of Beckman Coulter Inc Beckman Coulter Inc Warranty and Returned Goods Requirements All standard Beckman Coulter Inc policies governing returned goods apply to this product Subject to the exceptions and upon the conditions stated below the Comp
175. n good condition Replace as needed refer to Section 2 6 1 Replacing Gripper Fingers Make sure all pins on the HDR Pin Tool Body are clean and straight Note Dirt or grease residue on pins could result in poor liquid transfer results Keep pins and the pin plate clean Note Bent pins could result in poor liquid transfer results or labware crashes Replace any bent pins immediately refer to Section 2 3 1 3 Replacing Bent or Damaged Pins Multichannel Pod 2 21 2 6 Troubleshooting WARNING Do not connect or disconnect any cable while power is UN applied to the Biomek FX Perform the troubleshooting techniques provided in Table 2 4 when necessary Note In the case of any other pod related problems contact a Beckman Coulter Service Engineer Table 2 4 Multichannel Pod Troubleshooting If Then Power is lost to the pod Contact a Beckman Coulter Service Engineer The gripper is not deploying Check input air pressure Contact a Beckman Coulter Service Engineer Power is lost to the Y axis Contact a Beckman Coulter Service Engineer The head does not work properly Contact a Beckman Coulter Service Engineer Aspiration or dispense actions are not Contact a Beckman Coulter Service accurate Engineer A mandrel on the head is damaged Contact a Beckman Coulter Service Engineer the head may need to be returned to the factory for repair or replacement Liquid transfer operations w
176. n the desired probes to configure from the graphical representation of the probes 5 From the drop down menu choose the syringes in Probes 1 4 use syringes Note To choose probes 1 through 8 at one time hold down Ctrl with probes 1 through 4 highlighted and then click on probes 5 though 8 Note Probes 1 through 4 may be configured with one size of syringe and probes 5 through 8 may be configured with another size of syringe To mix the size of syringes select probes 1 through 4 and choose the syringe size Then select probes 5 through 8 and choose the syringe size 6 In Disabled Probes choose any probes that should not be used 7 Choose Accept Hardware Setup closes Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 16 Configuring the Biomek FX in Hardware Setup 4 4 2 Configuring a Speed Pump After a Speed Pump has been physically added to the Biomek FX instrument it must be added to the system configuration refer to Section 3 6 Speed Pump A Speed Pump is added to the system configuration through Hardware Setup To add a Speed Pump to the system configuration 1 Choose Instrument gt Hardware Setup to open Hardware Setup Hardware Setup appears Figure 4 11 Add Device Use Add Device to open New Devices Biomek Hardware Setup p Add Device me Remove Device J Accept gt lt Cancel jiomek FX SN None Serial Number fj AccuFram
177. nap into place when the tubing connector is attached Note Ensure that the tubing connector is attached to the Speed Pump so the tubing projecting from the distribution manifold to the pump assembly is attached to the Fluid Out port Fluid out port Fluid in port Overflow port Fluid passes from the Fluid passes from the Unused system fluid Speed Pump through the supply container passes through the Fluid Out port and into the through the Fluid In Overflow port on its distribution manifold for port and into the Speed way back to the supply the pump assembly A Pump container Tabs Squeeze the silver tabs to mount the tubing connector to the Speed Pump There are two tabs one on each end of the tubing connector Tubing connector Figure 3 36 Attaching the tubing connector to the back of the Speed Pump 10 the system fluid 11 12 Run the tubing connected to the Fluid In port to the supply container containing Cut the length of the Fluid In tubing so no excess tubing is used Run the tubing connected to the Overflow port to the supply container Note The supply container must be positioned below the height of the Speed Pump 13 Cut the length of the Overflow tubing so no excess tubing is used and no rises or bumps occur between the Speed Pump and the supply container The Speed Pump is installed Beckman Coulter Inc Span 8
178. nd Sensitivities 4 21 restoring settings 4 35 saving setting 4 34 Span 8 Pod properties in 4 31 understanding the options in 4 3 using Accept in 4 3 using Change Workspace in 4 3 HDR Tool Body installing on Multichannel Pod 2 16 interchangeable pin plates 2 6 installing 2 6 removing 2 8 heads changing 2 10 installing 2 13 overview 1 7 removing 2 11 homing all axes of a pod 6 3 pod 5 5 l indicator lights defined 1 6 instrument graphic of 1 3 L light curtain defined 1 9 maintenance 1 12 status indicator lights 1 6 Liquid Level Sensing LLS defined 3 6 disposable tips and 3 20 ensuring accuracy 4 21 fixed tips and 3 13 how to disable 3 7 3 8 performing Find Sensitivities 4 21 sensitivity settings 3 6 3 7 tips supported 3 6 M Manual Control accessing 6 2 defined 6 1 dialog display 6 1 6 2 using 6 2 Manual Teach using 5 15 Move Z Max using in Advanced Manual Control for a Multichannel Pod 6 9 for a Span 8 Pod 6 17 movement vectors understanding for a Multichannel Pod 6 12 understanding for a Span 8 Pod 6 19 Multichannel Pod control modes 2 4 current position displayed as coordinates 6 9 defined 2 1 installing HDR Tool Body 2 16 main components 2 3 movements 2 4 moving to a safe roving height 6 9 performing absolute moves for 6 13 performing relative moves 6 10 wrist ground strap changing heads 2 11 O open architecture instrument design concept 1 1 optional devices defined B 1 P pas
179. nd property damage properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies Contact an Beckman Coulter service representative for any servicing of equipment requiring the removal of covers or panels High Voltage This symbol indicates the potential of an electrical shock hazard existing from a high voltage source and that all safety instructions should be read and understood before proceeding with the installation maintenance and servicing of all modules Do not remove system covers To avoid electrical shock use supplied power cords only and connect to properly grounded three holed wall outlets Do not use multiplug power strips Disposal of Electronic Equipment It is important to understand and follow all laws regarding the safe and proper disposal of electrical instrumentation The symbol of a crossed out wheeled bin on the product is required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates that e the device was put on the European Market after August 13 2005 e the device is not to be disposed via the municipal waste collection system of any member state of the European Union For products under the requirement of WEEE directive please contact your dealer or local Beckman Coulter office for the proper decontamination information and take back program which will fac
180. ng and ferrule attachment to the tubing connector s ssssssessssseseessssesesserees 3 50 Figure 3 36 Attaching the tubing connector to the back of the Speed Pump cesses 3 51 Figure 4 1 Hardware Setup for a dual pod system with a Multichannel Pod and Span 8 Pod 4 2 Figure 4 2 Warning after choosing Home All Axes ss ssssssesssssssessseseesessesestsseseessesersesseserse 4 3 Figure 4 3 Hardware Setup configuration for Biomek FX essessessssesssrsersessrsresrssrreessrsersesse 4 5 Figure 4 4 Hardware Setup showing the configuration view for a Multichannel Pod 4 7 Figure 4 5 Warnings must be addressed before homing process begins ccsceeseeeeeteees 4 7 Figure 4 6 Hardware Setup displaying Enable Plate Sensor 0 0 0 cc ceccceseceeeeeeeeeteeeeteeeeeeeees 4 11 Figure 4 7 Error for no plate sensed icicasscseavassncsaccavsisodeaasspsecaansbindwnde tncdiaestadxsanshadcaelaaccanbasancanss 4 11 Figure 4 8 Hardware Setup showing the configuration view for a Span 8 Pod eee 4 13 Figure 4 9 Warning on Hardware Setup that pod must be homed and Find Sensitivities performed when tip types are changed c eeee 4 14 Figure 4 10 Warning that pod must be homed and Find Sensitivities performed when tip types are changed ccccccecsseceseceeeeeeeeeeeeceeeceseeeeeeeeseees 4 15 Figure 4 11 Hardware Setup lt ssccasssssviensssaccaaspsacaceaass saivarsnsnceandaaaevsiandaciaeasab
181. nianalenvasmneRivn 3 16 Figure 3 10 Fully installed fixed tip sz cscwecscscasssanenscasedanaiannednccesnsedesemenseacseesacecanantescavuesacencatveees 3 17 Figure 3 11 Pulling the collar off the fixed tip sccascssscisesnissecvasiscextanssscivassstectanseaviasassiccanaizaniarss 3 18 Figure 3 12 Removing a fixed tip sssessnssesesseseseessesesssesreserssressessrssressesstssressessteseesseeseesenssrt 3 19 Figure 3 13 Inserting a disposable tip mandrel into the tubing ssssssssesssseseesssssessssersrssrsee 3 20 Figure 3 14 Passing collar over a disposable tip mandrel ccccsseceseceeeeeeeeeeeeeeeeeteeeees 3 21 Figure 3 15 Installing a tip shuck tube on a disposable tip mandrel ceseeseeseereeeeeeeees 3 22 Figure 3 16 Installed disposable tip mama ssissscsscvnstesrsionnssenssusesuvvocnssnusesesdsseninsssdaussesusaveovasens 3 23 Figure 3 17 Fully installed disposable tip mandrel ccccceeceeceeesceesceceteceeeeeeeeeeeeceaeenteenees 3 24 Figure 3 18 Removing a tip shuck tube from a disposable tip mandrel cc eeeeeeeeeeeeeeees 3 25 Figure 3 19 Removing the collar from a disposable tip mandrel eeeeeeeeeseeseereeeeeeeeeeeees 3 26 Figure 3 20 Detaching a disposable tip mandrel from the probe tubing cee eeeeeeeeeeeeees 3 27 Figure 3 21 Span 8 pump assembly ixccesccsseccesanssersnancagecsustvecwsundcevaiaentencsveamaranansyseauaedsavenntieess 3 28 Figure 3 22 Pump ad Syringe sssr
182. nstrument Biomek FX and FX P Laboratory Automation Workstations User s Manual 1 10 Biomek FX Introduction A A Beckman Coulter Inc WARNING Either safety shields or light curtains must be installed along the front and sides of the Biomek FX instrument to provide the required safety The Biomek FX operates with a force that could cause injury if a hand is in the way during the loading of tips or other movement of the pipetting head The safety shields and light curtains prevent entry into the work area during machine movement WARNING To reduce the risk of personal injury the safety shields or light curtains must be in place before operating Configuration options for the perimeter protective system include e Front light curtain with both left and right side light curtains e Front light curtain left side light curtain and right side safety shield e Front light curtain right side light curtain and left side safety shield e Front light curtain with both left and right side safety shields 1 2 5 1 Light Curtain Protection System WARNING Dark non reflective material affects the sensitivity of the light curtain and adversely impact its effectiveness Typical lab dress such as lab coats and latex gloves do not degrade light curtain operation however it is advisable to test the impact of the all lab dress on light curtain sensitivity before operating the Biomek FX Verify lab dress impact on light curtain sensit
183. o both the Biomek FX and FX instruments The Biomek FX Laboratory Automation Workstation Figure 1 1 is a multiaxis liquid handling instrument used in the drug discovery laboratory The open architecture design along with the extensible operating software provides a foundation for integrating current and future specific use components The design of the Biomek FX provides the foundation for continual evolution and expansion of liquid handling systems The Biomek FX is available as a single or dual pod instrument Different operating components can be installed on each pod providing options for performing a variety of functions sequentially or simultaneously depending upon desired tasks and configurations A variety of labware and hardware adapt the deck of the Biomek FX to accomplish multiple tasks ranging from performing simple labware positioning and liquid transfers to completing complex activities that typically require additional devices in the laboratory The capabilities of the Biomek FX depend on the components installed including the pod s and head s ALPs and third party devices The chapters in this user s manual are arranged according to the components of the Biomek FX e Biomek FX instrument basics this chapter Multichannel Pod Chapter 2 e Span 8 Pod Chapter 3 e Configuring the Biomek FX in Hardware Setup Chapter 4 Framing the Biomek FX Chapter 5 e Manually Controlling the
184. o not perform aspirate or dispense operations without mandrels installed and tubing attached to fixed tips To operate the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface PPPE PPP Biomek FX and FX P Laboratory Automation Workstations User s Manual x Safety Information WARNING SPILL HAZARD WARNING Use an appropriately contained environment when using hazardous materials WARNING Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety officer WARNING Observe cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials WARNING Always wear protective gloves when removing the tips CAUTION Do not perform aspirate or dispense operations without mandrels installed and tubing attached to disposable tips To operate the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface CAUTION Do not overtighten the tip shuck tube Problems with unloading tips may result CAUTION Do not run syringes more than a few cycles without fluid in the syringes CAUTION Do not kink tubing between the supply container the Span 8 pumps and the Speed Pump CAUTION Do not kink tubing between the waste container the Span 8 Pod pumps and the Span 8 Tip Wash ALP CAUTION The syst
185. ockwise from the bottom of the tip interface Beckman Coulter Inc Span 8 Pod 3 27 4 Pull the collar down and off the disposable tip mandrel Figure 3 19 Probe Tip Interface Disposable Tip Mandrel Collar Figure 3 19 Removing the collar from a disposable tip mandrel 5 Gently pull the tubing and disposable tip mandrel down from the tip interface until enough tubing is extended to grasp between thumb and forefinger Figure 3 20 Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 28 Span 8 Pod 6 While holding the disposable tip mandrel in one hand gently pull the tubing off the mandrel with the other hand Figure 3 20 Note Since the tubing is Teflon it may be difficult to hold Holding the tubing with a Kimwipe may prevent slipping Note Carefully twisting the mandrel while pulling on the tubing may ease removal of the mandrel Probe Tip Interface Tubing Disposable Tip Mandrel Figure 3 20 Detaching a disposable tip mandrel from the probe tubing 7 Repeat steps through 6 for any other probes requiring disposable tip mandrels Beckman Coulter Inc Span 8 Pod 3 29 3 4 Pump Assembly The pump assembly comprises eight pumps and syringes one for each of the eight probes in the Span 8 Pod Figure 3 21 The pumps and syringes
186. od Note A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tips on the Span 8 Pod refer to Section 3 3 4 Electro Static Discharge Protection when Changing Tips on the Span 8 Pod Span 8 Pod 3 15 To install fixed tips 1 Place the end of a fixed tip into the tubing that extends from the bottom of the Span 8 probe Figure 3 3 2 While holding the tip in one hand push the tubing down over the tapered end of the tip until it is flush with the flat portion of the tip with the other hand Make sure the tip is secure to prevent air entering the system and fluid leaks around the tip Note The ends of the tubing may become damaged or stretched after repeated tip installation and removal It may be necessary to cut a small portion 1 2 of tubing off the end prior to attaching to ensure a tight fit Note Since the tubing is Teflon it may be difficult to hold Holding the tubing with a Kimwipe may prevent slipping while pushing the tubing down onto the tip Probe Tip Interface Tubing Fixed Tip Figure 3 7 Inserting a fixed tip into the tubing 3 Gently push the tubing and tip up into the Span 8 probe Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 16 Span 8 Pod 4 Pass the collar up over the tip to the tip interface Figure 3 8 Probe
187. od mounted on the right side of the Biomek FX instrument can only access ALPs on the right side of the deck The sections in this chapter include e Pod Section 2 2 Interchangeable Heads Section 2 3 e Gripper Section 2 4 Preventive Maintenance Section 2 5 Troubleshooting Section 2 6 Biomek FX and FX P Laboratory Automation Workstations User s Manual Multichannel Pod 2 2 Multichannel Pod gripper extended NW j A Figure 2 1 Multichannel Pod installed on Biomek FX o IE 5 O O c xX O a Multichannel Pod 2 3 2 1 1 Main Components of the Multichannel Pod The main components of the Multichannel Pod are Figure 2 2 e Pod Section 2 2 Houses operating mechanism pneumatic air line communication and electrical power connections to the base unit and moves in the Y Z and D axes for liquid handling functions Interchangeable Heads Section 2 3 Holds mandrels and tips for performing full plate replication e Gripper Section 2 4 Grip labware along the long side of the labware Pod Mandrels Grippers Figure 2 2 Multichannel Pod main components Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 4 2 2 Multichannel Pod Pod Beckman Coulter Inc The Multichannel Pod is a full micro
188. ol Body re E A E 2 18 Figure 2 14 GrPpET a casey sacs canescens a A E E EERE ER 2 19 Figure 2 15 Installed replaceable gripper fingers sicssscsswscecenassconcchaxsoencsesincaninasesssavandsnedeacdians 2 22 Figure 2 16 Removable front gripper assembly cccsccsssssssssssscssscssccssscsscesscsssassneceees 2 23 Figure 3 1 Span 8 Pod installed on a single pod Biomek FX instrument eceeeeeeeeeeeeees 3 2 Figure 3 2 Span Pod detailed View seed csessnaceresencaaccassadecaivsenssaenvadseacenacaatuneeakseckeantaaceaemstataies 3 3 Figure 3 3 Fixed tip height and width restrictions is ccisissscdacossicnnssaatcsgensasnadanesesteaesnennianscednencaves 3 11 Figure 3 4 Disposable tip height and width restrictions ccccsccesseeeeeesceeeeeceeeeeeeeeeseeesseenes 3 11 Figure 3 5 Disposable tip and tip shuck tube width restrictions with 10mm test tubes 3 12 Figure 3 6 Wrist ground strap attached to the deck cee eeeeceeeeseeceeeceseceeeeaeeeneseeeeseceeeeaeenaes 3 13 Biomek FX and FX P Laboratory Automation Workstations User s Manual XX List of Figures Figure 3 7 Inserting a fixed tip into the tubing 3 ssocsiecss besiedsodssnseradhacascaisdestuastasensienmarsesinacerades 3 14 Figure 3 8 Passing collar over fixed tip s ss sesessnssesessssesseserssresseserssressessrssressesseeseesseeseesenssre 3 15 Fig t 3 9 Tnstalled Hed Up 4 sascsawancsvasnsansaassannncsayiceuasetwiandiaivaviauidwnecdeanaaie
189. on 6 5 4 Setting Valve States Section 6 5 5 Verifying Liquid Level Sensing Section 6 5 6 Purging Air from the Syringes and Tubing Section 6 5 7 Many activities performed in Advanced Manual Control are the same for 6 15 Using Advanced Manual Control with the Span 8 the Multichannel Pod and the Span 8 Pod however the dialogs are different and the buttons for performing these activities may be placed in different areas Home Z XY Move Z Max Purge Set Valve States Get Tip Absolute Move Moves Z and then Moves pod to Syringes Sets valves on the Status Builds a vector to p X and Y axes to the highest Removes air ump to allow system Indicates an absolute g pump y home positions configured from the fluid to input output or which coordinate from p g p height tubing and bypass the syringes probes have the current syringes tips coordinate Ve ctor Adya ed a a 0 0 Pod a gt Buil d er ft Home Z xY Move Z Max Purge Syringes Set Valve States Get Tip Status Absolute Move G buto Clear Clear Refresh Used to build Vector Builder Current Position Xfcm Y cm Span mm iti a movement g back we po Fe P Displays the current position vector J 0 of the selected pod controls the Left 4 pa gt Right r were Z cm Tee 138 TE 138 TEC 136 fitiss ose 5 561 5 5e1 5 561
190. on 6 5 7 Purging Air from the Syringes and Tubing 3 5 5 3 Purging the Tubing and Syringes of Air CAUTION Do not purge the system without mandrels installed and AN tubing attached to disposable or fixed tips To purge the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface To accurately transfer liquid using a Span 8 Pod air must be purged from the tubing and syringes prior to running a method The tubing and syringes must also be purged of air after new tips or syringes are installed The purging process draws system fluid through the tubing and syringes until all air bubbles are removed from the tubing and syringes Purging the tubing and syringes of air is accomplished through Manual Control refer to Section 6 5 7 Purging Air from the Syringes and Tubing Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 48 Span 8 Pod 3 6 Speed Pump The Speed Pump Figure 3 33 is an optional device used to increase the speed of the tip washing process in the Span 8 Tip Wash ALP refer to the ALPs User s Manual Chapter 18 Span 8 Tip Wash ALP The Speed Pump propels system fluid through fixed tips at a rate of speed greater than can be supplied by the pumps and syringes The increased speed when used in conjunction with the Span 8 Tip Wash ALP is sufficient to clean the inside and outside of any fixed tips The flow of system fluid is controlle
191. ose Advanced Manual Control 9 Choose Exit to close Manual Control Beckman Coulter Inc ally Controlling the Biomek FX in Biomek Software 6 11 6 4 3 1 Setting Delta Values for the Multichannel Pod A Delta value is the amount of change in an axis that is applied to the Movement Vector when a button in the Vector Builder is pressed For example if the Delta value for X is 3 each time Right is clicked in the Vector Builder 3 cm is added to the X axis of the Movement Vector Note The move does not occur until Go is selected in the Movement Vector area of Advanced Manual Control To set Delta values 1 Choose Insturment gt Manual Control Manual Control appears 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Multichannel Pod appears Figure 6 9 Enter a value in X Y Z and D to set the Delta value for each axis In D Units select cm or pL Choose Close to close Advanced Manual Control for the selected pod 4 5 6 7 Biomek FX and FX P Laboratory Automation Workstations User s Manual Choose Exit to close Manual Control 6 12 Beckman Coulter Inc Manually Controlling the Biomek FX in Biome 6 4 3 2 Understanding and Using the Vector Builder for the Multichannel Pod The use of Advanced Manual Control for the Multichannel Pod centers around the building and applying of movement vectors A movement vector simply indicates the magnitud
192. oved from multichannel head Note Itis very important to remove the plunger screws in the correct sequence before removing the shoulder screws in the correct sequence Beckman Coulter Inc Multichannel Pod 2 13 Figure 2 8 Sequence for removing four plunger screws and four shoulder screws on all multichannel heads 4 Slide the head down and out of the pod Due to the weight of the head approximately five pounds maintain a firm grasp on the head while sliding the head out 5 Cover and store the head flat side down in a clean cool dry place 2 3 2 2 Installing Multichannel Heads CAUTION To prevent damage due to electrical static discharge ESD AN wear a wrist ground strap when installing a multichannel head Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek FX instrument The same method is used to install the 96 Channel 20 uL Head 96 Channel 200 uL Head and 384 Channel 30 uL Head Note Refer to Section 2 3 2 3 Installing an HDR Tool Body for instructions on installing the HDR Tool Body To install a head 1 In Hardware Setup select the appropriate Multichannel Pod Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 2 Choose Change Head Note Choosing Change Head moves the D axis to 0 6 cm
193. p and should not be repeated 4 9 Configuring the Biomek FX in Hardware Setup 4 3 3 Most of the properties including pod settings and axes limits of a Multichannel Pod are initially configured by a Beckman Coulter Service Engineer in Hardware Setup and should not be modified without specific instructions from a Beckman Coulter Service Engineer or Beckman Coulter Technical Support New D and Z axis limits must be established however when switching between the HDR Tool Body and a Multichannel head CAUTION Do not make any changes to the pod axes limits without contacting a Beckman Coulter Service Engineer or Beckman Coulter Customer Technical Support Setting Multichannel Pod Properties Table 4 2 describes the pod properties and axes limits listed in Hardware Setup for a Multichannel Pod Note Some of the fields listed in the following table see Table 4 2 may be accessed by choosing Additional Pod Settings and Tip and Gripper Settings in Hardware Setup Figure 4 4 Tip settings are not applicable to the HDR Tool Body Table 4 2 Multichannel Pod Properties Property Additional Roving Height Description Margin above the default height for the pod as it moves over everything on the deck Additional Timeout Specifies a number of seconds to wait in addition to the normal time it takes to carry out a step before a timeout error occurs Use when experiencing timeout problems without actual hardware problems
194. pan 8 Pod 6 18 6 5 4 Performing Absolute Moves for the Span 8 Pod 6 20 6 5 5 Setting Valve States 66 dk eae hse een teens owas bene yebauas 6 21 6 5 6 Verifying Liquid Level Sensing o k sid saadedentacceannedeliad 6 22 6 5 7 Purging Air from the Syringes and Tubing 6 22 6 6 Using Advanced Manual Control to Manually Control Speed Pumps 6 24 Beckman Coulter Inc WF 10 Woy m Oxo ai c acy xix Appendix A Specifications 0 0 00 cee A 1 Appendix B Using a Stacker Carousel 0000 0c eeuee B 1 Bl OVVIE Waride ta pti dnth dan REA PREAH T E RG RRREE RARE B 1 B 2 Integrating the Stacker Carousel wis 4 cs cdcoek daddaatso4N444 8s ia din iws eke B 2 B 2 1 Positioning the Stacker Carousel on the Deck 4 B 3 B 2 2 Mounting the Stacker Carousel 0 0 0 0 cc cece eee ee eee B 5 B 2 3 Attaching the Side Shield ccc sc teeh coven ens eb RGSS GAR eee B 8 B24 Operating the Stacker Carousel ovedca nad headedea Vaden eee ee nase B 12 B 2 5 Preventive Maintenance and Troubleshooting B 12 B 3 Integrating the Bar Code Reader 0 ccc ccc eens B 13 B 3 1 Attaching Bar Code Reader Bracket 0 00 c eee eee ee B 13 B 3 2 Attaching Bar Code Reader to Shuttle 00000 B 14 B 3 3 Positioning Bar Code Reader 25nc 52hcaeetatcus nee bes eaded B 16 B 3 4 Applying Power to
195. plate replication tool incorporating a gripper and interchangeable heads to accommodate a variety of functions 2 2 1 Pod Movements The Multichannel Pod performs movements in the Y Z and D axes see Table 2 1 Table 2 1 Multichannel Pod Axes Movement Y Entire pod moves front to back Z Entire pod moves up and down D Up and down aspirate dispense disposable tip shucking and close open gripper 2 2 2 Control Modes Commands entered via Biomek Software on the host computer control the operations of the Multichannel Pod Manual control associated with the operation of the Multichannel Pod is accessed through Biomek Software Note Refer to Chapter 6 Manually Controlling the Biomek FX in Biomek Software for information on manually controlling the Multichannel Pod Multichannel Pod 2 5 2 3 Interchangeable Heads An interchangeable multichannel head is attached to the bottom of the pod to perform a specific liquid handling procedure Depending on the head and the desired liquid handling procedure different tip types may be used Presently there are four types of heads that can be attached to the Multichannel Pod e 96 Channel 20 uL Head e 96 Channel 200 uL Head e 384 Channel 30 uL Head e HDR Tool Body Interchangeable heads installed on the Multichannel Pod aspirate and dispense liquid using disposable tips The tips compatible and the maximum volume that may be aspirated and dispensed wit
196. pt gt lt Cancel adba fisesh Serial Number fa AccuFrame 96 Podi Port Simulate 7 8 Pod2 paves JIV This is a dual armed system 7 DeviceControllerO 47 ShakerALPO Left Pod Type flere Multichannel Pod 7 oF Shaker LP1 oF SpeedPumpo Right Pod Type Right Span 8 Pod x 47 SpeedPump1 oF StirrerALPO 47 StirrerALP1 oF TiploaderO 47 Tiploader1 Digital Devices J WashPump1 a e Simulator e Camera E Materials f Stacker Carousels Figure 4 3 Hardware Setup configuration for Biomek FX Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 6 Configuring the Biomek FX in Hardware Setup 4 3 Configuring a Multichannel Pod A A Beckman Coulter Inc The Multichannel Pod is a full microplate replication tool incorporating a gripper and interchangeable heads to accommodate a variety of functions refer to Chapter 2 Multichannel Pod A Multichannel Pod may be installed on the right left or both bridges of the Biomek FX Configuring a Multichannel Pod in Hardware Setup includes e Configuring a New Head Section 4 3 1 Note An HDR Tool Body is configured as a head in Hardware Setup e Correlating the Pods Section 4 3 2 Note Correlating the pods should be performed only by a Beckman Coulter Service Engineer at initial setup e Setting Multichannel Pod Properties Section 4 3 3 as instructed by a Beckman Coul
197. pumps are set to bypass and the unit is not dispensing fluid to the probes Contact a Beckman Coulter Service Engineer The Speed Pump is leaking from an area other than a tubing connection Contact a Beckman Coulter Service Engineer The Speed Pump is leaking from a tubing connection Cut off approximately 1 2 of tubing to remove the damaged portion before reattaching the tubing Note In the case of any other pod related problems contact a Beckman Coulter Service Engineer Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 1 Configuring the Biomek FX in Hardware Setup Overview The Biomek FX Laboratory Automation Workstation must be configured in Biomek Software to ensure proper operation Hardware Setup tells Biomek Software what devices pods and heads to expect on the instrument by providing a connection between the instrument and the software This connection is established by installing configuring and removing devices in Hardware Setup After a device has been physically installed the device is detected on the Biomek FX instrument and must be properly installed and configured in Hardware Setup While a new device is normally installed and configured in Hardware Setup when the device is installed it also may be necessary to install configure and remove other devices using Hardware Setup Setup without contacting a Beckman Coulter Service Engineer or
198. put while low voltage devices receive power through the Controller Area Network CAN interface located on the back of the Device Controller Some ALPs and devices require a Device Controller to use them on the deck of the Biomek FX Note Refer to the ALPs User s Manual Appendix A Device Controller for detailed information about connecting and controlling devices using a device controller Beckman Coulter Inc Biomek FX Introduction 1 9 1 2 5 Protective Barriers A perimeter protective safety system is standard for the Biomek FX instrument This safety system helps protect against operator injury damage to the equipment and interruptions during the liquid handling process The standard Biomek FX perimeter protective system includes a diffuse reflective light curtain along the front of the instrument and either diffuse reflective side light curtains or protective side safety shields along the left and right sides of the instrument Components of the perimeter protective safety system may include e A diffuse reflective perimeter light curtain along the front edge of the Biomek FX instrument e A diffuse reflective perimeter light curtain along either or both sides of the Biomek FX instrument e Side safety shields e An overhead canopy Side light curtain O A en en oe R A N Front light curtain A Figure 1 5 Protective barriers for the Biomek FX i
199. r s Manual B 6 Using a Stacker Carousel Beckman Coulter Inc Also included with the alignment bracket is the following mounting hardware e Two 1 4 20 x 1 long stainless steel socket head cap screws e Two 1 4 flat washers e Two 1 4 lock washers e Upper alignment bracket e Two 1 4 20 x 1 long stainless steel flat head cap screws B 2 2 1 Attaching Alignment Brackets Attach the upper alignment bracket the right or left alignment bracket and the riser plate to prepare for positioning and leveling the Stacker Carousel as follows 1 Choose the right or left side of the Biomek FX instrument on which the Stacker Carousel will be located 2 Choose the deck position the shuttle will occupy Figure B 2 Note Position P1 or P17 back mount or Position P2 or P18 front mount 3 Remove the deck plate from the Biomek FX deck 4 Attach the upper alignment bracket to the Biomek FX deck in the appropriate base mounting holes for front or back mounting Figure B 6 5 Using the appropriate mounting slots Figure B 4 or Figure B 5 attach the right or left horizontal alignment bracket to the upper alignment bracket 6 Adjust the height of the horizontal alignment bracket so that the bottom surface of the bracket is sitting flush on the surface on which the Biomek FX is mounted then tighten the fasteners Figure B 6 Use front two base P viN g mounting holes for se middle two base front mount
200. r bent pins 1 Remove the pin plate from the HDR Tool Body refer to Section 2 3 1 2 Removing Pin Plates 2 Place the populated pin plate with pins pointing down in a 96 well microplate for a 96 pin plate or a 384 well microplate for a 384 pin plate 3 Remove any damaged or bent pins by lifting them out of the pin plate 4 Drop the replacement pin gently into position 5 Reinstall the pin plate on the HDR Tool Body refer to Section 2 3 1 1 Installing Pin Plates 2 3 1 4 Storing HDR Pins To store HDR pins CAUTION Always store pins in the pin plate or pin storage case to AN prevent pins from getting damaged or bent 1 Place the populated pin plate with pins pointing down in a 96 well microplate for a 96 pin plate or a 384 well microplate for a 384 pin plate 2 Put on the pin capture plate to keep pins from falling out 3 Return the pin plate and pins to the original packing materials and store in a dry dust free environmentally controlled area Note It is desirable to allow the pin plate and pins to air dry before returning them to the original packing materials Biomek FX and FX P Laboratory Automation Workstations User s Manual 2 10 Multichannel Pod Beckman Coulter Inc 2 3 2 Changing Heads Each Multichannel head can be removed and replaced to accommodate the needs of a particular method Note When a head is changed the Hardware Setup must be changed appropriately If the hardware configu
201. rame framing tool ennienni a a 5 2 Figure 5 2 Warning to address before homing process begins ssssssessessssessesessresseseresesse 5 4 Figure 5 3 Framing Pagar escssssvsasvsnsysadesasesebnendes ign EA Asire EEA EEEE EE TAARE EEES EEE RRE 5 5 Fig re 5 4 HDR Framing Se sisri ea a a RS a 5 6 Figure 5 5 Fastening HDR Framing Fixture to the HDR Tool Body ssssssessesessessererssrsersesse 5 7 Figure 5 6 Framing a Span 8 PO sac ssscievanessscuncsenavesseanaegnncnsnecosenabaicnscenscaveateetsnesanedasenessaseassvaeeiee 5 8 Figure 5 7 Attaching a Framing Shaft details eeeecessescesseeeeeeeeceseceaecaeeeneeeeeseeesecaeeneeees 5 9 Figure 5 8 Deck EditOt ope cays naiceccciu tinrin rine a DEA ses eed tea ado TERE E EATE 5 11 Pils 5 9 Position PIOPCTUES wintsscixenssnunsarexnarenvansnanvsacdbucnes iais E EE EEEE EANAN EE 5 12 Figur 5 10 CONi A Ree rere eae Re saa E ER area ia aia Eta edirs eee 5 12 Figure 5 11 Teaching InStrUCtionS nissersiisnsssiesaiia n iani E EEEa ERE EEE 5 13 Fig r 5 12 Deck Editor cvssanysusessaonewsnaasaduceduinsnsesia ssleseadiaassaunnenianidenistaansiaseaaennteiaaanenieuasnncain 5 15 Figure 5 13 Position Properties for a Positive Position ALP s sesssssssesssssssseseseestsresesseseesse 5 16 Figure 5 14 Manual Teaching Warning cc udc5cacssncasncessocvnteinss sasasseucedassensia Gates ates iavantess isabeasels 5 16 Figure 5 15 Manual Teaching if tips are not already loaded ec eeeeeceese
202. ransfer step using a Span 8 Pod Configuring the Biomek FX in Hardware Setup 4 21 4 4 3 Correlating the Pods CAUTION Do not use Correlate Pods in Hardware Setup without UN contacting a Beckman Coulter Service Engineer or Beckman Coulter Technical Support To avoid collisions in a dual pod system the pods must be correlated This procedure is performed during initial setup by a Beckman Coulter Service Engineer in Hardware Setup and should not be repeated 4 4 4 Correlating the Z Axes CAUTION Do not use Correlate Z Axes in Hardware Setup without UN contacting a Beckman Coulter Service Engineer or Beckman Coulter Technical Support To eliminate mechanical variances the Z axes on the probes must be correlated This procedure is performed during initial setup by a Beckman Coulter Service Engineer in Hardware Setup and should not be repeated 4 4 5 Performing Find LLS Sensitivities Performing Find LLS Sensitivities is necessary to test each probe on an individual system to ensure the liquid level sensing capability operates properly This procedure adjusts the system to the individual liquid level sensitivity settings on each probe Although Find LLS Sensitivities is performed during initial setup by a Beckman Coulter Service Engineer the procedure must be repeated when e Changing from disposable to fixed tips e Changing from fixed to disposable tips e Difficulties such as false trips or failed senses are exper
203. rate of speed greater than can be supplied by the pumps and syringes for increased tip washing efficiency m l l Probes Span 8 Pod l l l Probes M PP I e k f M i Tip interface gt Tips are attached ne to the probes at I the tip interface Figure 3 2 Span 8 Pod detailed view Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 4 Span 8 Pod 3 2 Probes The Span 8 Pod uses eight probes that can move independently in the Z axis and pipette independently in the D axis with the assistance of the pumps Movement in the S axis provides a span between the probes The pipetting action of the Span 8 Pod is accomplished using either fixed or disposable tips attached to the tip interface of the probes These tips may or may not be liquid level sensing capable refer to Section 3 3 Interchangeable Tips Note The Span 8 Pod can perform liquid level sensing LLS during any liquid transfers but only if liquid level sensing tips are attached to the probes 3 2 1 Probe Movements The Span 8 Pod performs movements in the Y Z D and S axes refer to Section 3 1 Span 8 Pod Axes Movement Table 3 1 Span 8 Pod Axes Movement Y The eight probes move simultaneously front to back Z The eight probes move up and down independently D The aspirate dispense action is controlled by the pumps S The span or distance between the
204. ration is not updated using Hardware Setup inaccurate pipetting may occur refer to Section 4 3 Configuring a Multichannel Pod 2 3 2 1 Removing Heads CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when removing a multichannel head Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek FX instrument Figure 2 5 Strap portion of wrist ground strap snugly attached to the wrist Alligator clip clamped to the instrument deck base plate Figure 2 5 Wrist ground strap attached to the deck The same method is used to remove the 96 Channel 20 uL Head 96 Channel 200 uL Head 384 Channel 30 uL Head and HDR Tool Body Removing a head from the Multichannel Pod includes removing the bottom cover e removing the head Multichannel Pod 2 11 2 3 2 1 1 Removing the Bottom Cover To remove the bottom cover to expose the head 1 In Hardware Setup select the appropriate Multichannel Pod Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 2 Choose Change Head Note Choosing Change Head moves the D axis to 0 6 cm and extends the grippers 3 Turn off power to the Biomek FX instrument 4 Position the strap portion of the wrist ground strap snugly around the wrist 5 Clamp the alligator clip of the wrist
205. reparing the Liquid System nnana nannan nanana 3 45 3 6 Speed PUMP 54h bear oa a teenth ae Oba ca egi ini aS eee eas 3 47 3 6 1 Speed Pump Specifications nnana Kade yeas Kees aeeea eens 3 49 3 6 2 Adding a Speed Pump to the Biomek FX Instrument 3 49 3 6 3 Setting Address Switches 0c cece ccc cece cece eee eeas 3 52 3 7 Preventive Maintenance os odavks vase ta evnesiae yee ekauseaNwese weed 3 53 3 8 NIQUBISSHOOUING y 4 d aid core G ae pee ee REO e oe Wee ERR Pew eS 3 54 Configuring the Biomek FX in Hardware Setup 4 1 CNGIVIEW le cna oc ehd chee Deen ee eee teen Hees eee E a E 4 1 4 1 1 Accessing Hardware Setup 0 0 ccc cece nunana 4 2 4 1 2 Understanding the Options in Hardware Setup 4 3 4 2 Configuring the Biomek FX Instrument 0 00000 cece eee 4 4 4 3 Configuring a Multichannel Pod nnana nnana aana 4 6 4 3 1 Configuring a New Head so i c a40064naesGedbndateesdeucdens 4 6 4 3 2 Correlating the POS censahuse ea cee keen eae ue awa eee en en aes 4 8 4 3 3 Setting Multichannel Pod Properties 0 000000 ee aes 4 9 4 4 Configuring a Span 8 P00 isi oscgedaives bodes rer wes des todutdedd 4 12 4 4 1 Configuring the Probes for Fixed or Disposable Tips 4 12 4 4 2 Configuring a Speed Pump veces eeeaveene eae ow eae een beens 4 16 4 4 3 Correlating the Pods c 400sbaccbn dente dade ead eee ease vue 4 21 4 4 4 Correlatin
206. rior to use The system must then be primed with the new system fluid and the Span 8 system tubing must be purged of air Refer to Section 3 5 5 Preparing the Liquid System for more information on degassing priming and air purging procedures Beckman Coulter Inc Span 8 Pod 3 39 3 5 1 1 1 Parts Along the Span 8 System Fluid Flow Path Each of the Span 8 system parts listed in Table 3 5 comes into contact with the system fluid and must be considered for possible susceptibility to the caustic nature of non supported system fluid Table 3 5 Parts and materials along the Span 8 fluid flow path Part Material Carboy Polypropylene Supply carboy tubing Tygon 2075 Carboy Cap Polypropylene Tubing cap to quick Tygon 2075 disconnect Quick Disconnect body Polypropylene EPDM and Stainless Steel Quick Disconnect insert Polypropylene EPDM and Stainless Steel Supply tubing Tygon 2075 Manifold Inlet fitting Polypropylene Manifold There are two styles of manifolds Teflon manifolds Part 717892 Polypropylene manifolds Part 719964 Tubing manifold to pump Teflon Stainless Steel and Polypropylene fittings should not contact liquid if tightened properly Pump valve Kel F Valve plug Virgin Teflon Syringe Glass Syringe piston seal Teflon Tubing pump to the tips Teflon Note Polypropylene fittings should not contact liquid if tightened properly Tips Fi
207. ritical as when using 384 well plates each position accessed must be framed by both pods Note If Pod 2 in the example above is framed before Pod 1 the Coordinates of Pod 2 do not change to match those of Pod 1 Note After teaching both pods the coordinates displayed for the two pods typically are slightly different Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 2 Framing the Biomek FX 5 2 Framing Deck Positions on the Biomek FX Using AccuFrame The AccuFrame is a tool used for framing ALP and labware positions on the Biomek FX deck Figure 5 1 Framing using the AccuFrame tool requires no human judgment of alignment and is reproducible AccuFrame light beams Gripper cutout Power light Gripper fingers are framed in the cutout at the bottom of both AccuFrame light sides of frame tool beam indicators Figure 5 1 AccuFrame framing tool The AccuFrame fits snugly on an ALP and a teaching process is performed through Biomek Software to obtain the coordinates for each deck position The framing is completed by breaking two light sensors on the AccuFrame at their intersection point The coordinates for each ALP are generated automatically through the software based upon teaching one position however when precision is critical as when using 384 well plates each pod must be used to frame each position before using
208. rom the microplate side to be scanned Figure B 18 through Figure B 28 Laser beam Figure B 18 Bar code reader in narrow side scanning position Beckman Coulter Inc N Ti a Using a Stacker Carousel Laser beam 19 Bar code reader in narrow side scanning position Figure B Laser beam 20 Bar code reader in wide right side scanning position Figure B T gt 2 1 2 2 0 5 2 7 4 pam 3 0 5 Cy E fe jt x E fe D 0 Q l 3 x lt LL xe Pa LL lt 2 aa B 18 Using a Stacker Carousel Laser beam Figure B 21 Bar code reader in wide right side scanning position bottom view Laser beam Figure B 22 Bar code reader in rear narrow side scanning position Beckman Coulter Inc Using a Stacker Carousel B 19 Laser beam Figure B 23 Bar code reader in rear narrow side scanning position bottom view Laser beam Figure B 24 Bar code reader in wide left side scanning position Biomek FX and FX P Laboratory Automation Workstations User s Manual B 20 Using a Stacker Carousel T i 7 Vy UU WANS Laser beam Figure B 25 Bar code reader in wide left side scanning position bottom view Figure B 26 Bar code reader in front
209. rtant It is your responsibility to decontaminate components of the Biomek FX before X requesting service by a Beckman Coulter service representative or returning parts to Beckman Coulter for repair Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so If any parts are returned they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated Beckman Coulter Inc Safety Information ix Warnings and Cautions Found in this Manual Please read and observe all cautions and instructions Remember the most important key to safety is to operate the Biomek FX with care The WARNINGs and CAUTIONS found within this document are listed below WARNING If the equipment is used in a manner not specified by Beckman Coulter Inc the protection provided by the equipment may be impaired WARNING Either safety shields or light curtains must be installed along the front and sides of the Biomek FX instrument to provide the required safety The Biomek FX operates with a force that could cause injury if a hand is in the way during the loading of tips or other movement of the pipetting head The safety shields and light curtains prevent entry into the work area during machine movement WARNING To reduce the risk of personal injury the safety shields or light curtains must be in place before operating WARNING Dark non reflective material
210. s pod or ALP Note Some ALPs such as the Positive Position ALP must be framed using Manual Teach to improve pipetting accuracy to high density labware Note When not using high density labware the standard framing procedure using the AccuFrame is acceptable To frame using the AccuFrame refer to Section 5 2 Framing Deck Positions on the Biomek FX Using AccuFrame To frame using Manual Teach 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software to open the Biomek Software 2 Choose Instrument gt Deck Editor Deck Editor appears Figure 5 12 Deck1 Default Deck D x i E a NewDeck Delete Deck Rename Deck Open Deck ClearDeck Renumber Delete ALP Properties Save Cancel BarcodeReaderLeft BarcodeReaderRight FourByFour OneByFive OneByOne OneByThree PositivePositionSLP ShakerALP Span8TipTrash Span8TrashLeft gt WS2 Span8TrashRight gaa Span washLeft Span washRight SPE SPE2x1Left 5 gt gt 13 Shakerl SPE 2x1 Right SPEHolder StackerCarousel zi Figure 5 12 Deck Editor Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 16 Framing the Biomek FX 3 Open Position Properties for the desired deck position by double clicking on the deck position Position Properties appears Figure 5 13 Name Post ALP Type PositivePositionALP x em Y em Z cm Precision Pod1 Coordinates 3 511 7 213 15 7 Not Framed Pod2 Coordinates 98 36
211. s powered on Disconnect power before removing or replacing a cover WARNING Avoid direct exposure to the laser beam Never look directly into the laser beam and never leave the laser on open or unattended WARNING Always have the laser module access cover located on the bar code reader in place when operating or troubleshooting the laser module CAUTION If labware other than that specified in the Stacker Carousel User s Manual is used an increase in bad reads or no reads may occur CAUTION If a label is applied by any means other than the Beckman Coulter Print and Apply device an increase in bad reads or no reads may occur CAUTION To prevent damage to the shuttle first place the AccuFrame into the Framing Tool Adaptor Then place the AccuFrame with the attached adaptor on the shuttle CAUTION Turn off power to the Biomek FX instrument before attaching or removing AccuFrame from the instrument deck CAUTION Use Manual Control to move the probes in the Y axis Dragging the probes by hand may affect alignment and the ability to access labware A A A A A A A A A A A Biomek FX and FX P Laboratory Automation Workstations User s Manual Table of Contents Safety Information 2 coud ene ne ee et ncee des anann see ee eeee oa ee v Warning and Caution Definitions i4 00254 sannana naasar eS GES REESE REAR ERRORS v Electrical Sa letys hd aes Se T a E E ie ee E E E vi High VOUASC lt 9 44scd vee 9
212. s resulting in incorrect f CAUTION Use Shift deck only before other ALPs or positions are coordinates if applied to previously framed ALPs and positions e Shift deck shifts all ALPs and positions associated with the deck by the change amounts shown Choose Shift deck when framing the first location of a new deck The shift amount required typically is not large but everything on the deck may need to move cm for example e Shift ALP shifts the entire ALP and all deck positions associated with the ALP by the change amounts shown Shift ALP typically is precise enough for using 96 well plates e Shift position shifts only the deck position containing the AccuFrame by the amounts shown Shift position is the most precise teaching procedure and it is useful when 384 well microplates are used especially on larger ALPs such as the 4x4 otherwise Shift ALP is usually sufficient Note When framing a multiple position ALP 1x3 1x5 4x3 or 4x4 Shift ALP on the first position then Shift position on the rest Beckman Coulter Inc Framing the Biomek FX 5 15 5 3 Manually Framing Deck Positions on the Biomek FX Manual Teach is a wizard type interface that is used manually frame deck positions primarily for using high density labware Since the wells of high density labware are relatively small using Manual Teach helps to ensure that the tips can access the wells without causing any damage to the tips probe
213. s which include the settings and axes limits of a Span 8 Pod are initially configured by a Beckman Coulter Service Engineer in Hardware Setup and should not be modified without specific instructions from a Beckman Coulter Service Engineer or Technical Support However pod properties should be adjusted when physical changes have been made to the Biomek FX instrument Always consult a Beckman Coulter Service Engineer before changing values Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 32 Configuring the Biomek FX in Hardware Setup CAUTION Do not make any changes to the axes limits and pod settings without consulting a Beckman Coulter Service Engineer A Table 4 3 describes the pod properties and axes limits listed in Hardware Setup for a Span 8 Pod Note Some of the fields listed in the following table see Table 4 3 may be accessed by choosing Additional Pod Settings in Hardware Setup Table 4 3 Span 8 Pod Properties Property Description Additional Roving Height Margin above the default height for the pod as it moves over everything on the deck Additional Timeout Specifies the number of seconds to wait in addition to the normal time it takes to carry out a step before a timeout error occurs Use when experiencing timeout problems without actual hardware problems Always Move to Z max when roving Check this field to move the pod to its maximum height during any move in
214. s MagBeadALP HW Address 00 oF StirrerALPO MagBeadALP HW Address 01 g7 StirrerALP1 MagBeadALP HW Address 02 g7 TiploaderO MagBeadALP HW Address 03 7 Tiploader1 OrbitalShakerALP HW Address 00 f Digital Devices OrbitalshakerALP HW Address 01 E Simulator OrbitalShakerALP HW Address 02 camera OrbitalshakerALP HW Address 03 FH Materials Tiploader HW Address 2 Stacker Carousels Tiploader HW Address 3 Figure 4 14 Right clicking on Devices to add a Speed Pump in Hardware Setup 8 Select the Span 8 Pod in the system hierarchy displayed in the left pane of the Hardware Setup Figure 4 15 The Span 8 Pod configuration appears in the right pane Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 20 Configuring the Biomek FX in Hardware Setup 9 Select a Pump for the pod in the pod configuration options displayed Figure 4 15 Note A Speed Pump propels system fluid through every probe on the Span 8 Pod not selected or individual probes BiomekFX Biomek FX SN None fj AccuFrame 96 Podi 8 Podz amp Devices DeviceControllerO g7 ShakerALPo g7 ShakerALP1 7 SpeedPumpo 7 SpeedPump1 oY StirrerALPO g7 StirrerALP1 47 TiploaderO 47 Tiploader1 Digital Devices BD Simulator E Camera Ep Materials f Stacker Carousels Probe Configuration Probes 1 4 us
215. s button Find Clot Detection Sensitivities J Start This wizard assists with determining the maximum sensitivity for each probe This will help minimize errrors created by receiving a false Load Tips detection Enap All ofthe probes will need to have tips loaded Any probe confiqured to use disposable tips will have to be loaded with a conductive tip The currenttip confiquration is Probe 1 Disposable tip P200_LLS No tip detected Probe 2 Disposable tip P200_LLS No tip detected Probe 3 Disposable tip P200_LLS No tip detected Probe 4 Disposable tip P200_LLS No tip detected Load Tips will Probe 5 Disposable tip P200_LLS No tip detected not be displayed Probe 6 Disposable tip P200_LLS No tip detected here if all probes Probe 7 Disposable tip P200_LLS No tip detected Probe 8 Disposable tip P200_LLS No tip detected are configured with fixed tips Figure 4 26 Find Clot Detection Sensitivities Start Note On the left side of Find Clot Detection Sensitivities a list of steps required to complete the procedure is displayed As the steps of Find CD Sensitivities are accessed they are highlighted on the left Note A Span 8 Pod that is configured with all fixed tips will not display Load Tips on the left side of Find Clot Detection Sensitivities Biomek FX and FX P Laboratory Automation Workstations User s Manual 4 28 Configuring the Biomek FX
216. s itself to the center of the large circle The values displayed in Total Moved from Start cm changes each time steps 10 through 15 are completed If desired the values in Total Moved from Start cm can be reset to zero by selecting Reset 16 Visually verify the position of the tips on the Biomek FX instrument in relation to the wells of the microplate on top of the ALP If the tips are still not accurately positioned above the microplate repeat steps 10 through 16 until they are accurately positioned above the microplate Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 20 Framing the Biomek FX 17 Choose Next and Figure 5 18 appears The tips should centered above the wells and 0 5 cm above the top of the plate They are going to go down 0 5 cm 0 5 cm above the well bottom If this would cause a collision press Cancel Cancel Figure 5 18 Manual Teach warning on tips descending into a microplate 18 Address the Warning and choose OK Teach Z appears Figure 5 19 Manual Teaching The tips should be 0 5 centimeters above the well bottom Lower the pod until the tips just touch the bottoms of the wells until the plate does not move when lifted then click Finish Warning Delta 0 05 em ra sx Left 2 ESPA Total Moved From Start cm Teach Z Figure 5 19 Manual Teaching Teach Z 19 In Delta select the magnitude of chang
217. se move the probes on the Span 8 Pod in the D axis based upon the selection made in D Units ally Controlling the Biomek FX in Biomek Software 6 17 6 5 1 Viewing the Current Position of a Span 8 Pod The current position of a Span 8 Pod is displayed as five coordinates The X Y Z and D axes coordinates are the distances from the home position The Span is the distance between the probes X Y and Z are displayed in centimeters the D axis is displayed in microliters and the Span is displayed in millimeters To view the current position of a pod 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Span 8 Pod appears Figure 6 12 The current position is shown in Current Position 4 Choose Close to close Advanced Manual Control 5 Choose Exit to close Manual Control 6 5 2 Moving a Span 8 Pod to a Safe Roving Height Use Move Z Max in Advanced Manual Control for a selected Span 8 Pod to move it to its highest configured height This helps to avoid collisions when moving the pod around the deck manually Note The possibility of collisions is not completely eliminated by Move Z Max 1 Choose Instrument gt Manual Control Manual Control appears 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Span 8 Pod appears Figure 6 12 4 Choose Move Z
218. se two pieces constitute the syringe Note A syringe reflecting the maximum volume most frequently aspirated and dispensed during a method should be installed in the pumps To assemble and install syringes 1 Remove the syringe components plunger and glass casing from their original packaging material 2 Moisten the rubber gasket on the end of the plunger with water 3 Insert the end of the plunger with a rubber gasket into the glass casing 4 Push the plunger into the glass casing until it stops Figure 3 23 The syringe is now assembled 3 lt _ Glass Casing Rubber gasket lt _ Plunger CC Gl Figure 3 23 Installing syringes assembling the syringe 5 Gently insert the top of the syringe into the valve Figure 3 24 Span 8 Pod 3 33 6 Screw the top of the syringe into the valve by turning the syringe to the right until finger tight Figure 3 24 Note Make sure the syringe is secure to prevent air entering the system and fluid leaks around the syringe lt Pump Valve Top of the Syringe Syringe Base of the Syringe Thumbscrew Figure 3 24 Installing syringes push the syringe up into the valve and screw it in Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 34 Span 8 Pod 7 Push the t
219. sition in the X axis based on the position of the bridge at the back of the Biomek FX instrument Figure 5 17 Manual Teaching 10 To align the tips in the X and Y axes with the wells of the microplate on top of the ALP lower the tips in the Z axis until they are approximately 1 mm above the top of the ALP Note Since tip height is set in the next step in the Manual Teaching process it is safe to move the pod to any height to make aligning the tips with the microplate easier 11 Visually verify the physical position of the tips in relation to the physical position of the wells of the microplate on top of the ALP Beckman Coulter Inc Framing the Biomek FX 5 19 12 Select Well Corner to align the tips to the corners or junction of four wells Note Well Corner is available only when framing to a deck position occupied by a piece of labware with square wells OR Select Well Center to align the tips to the center of the wells 13 In Delta select the magnitude of change applied to the tips each time a directional button is selected Figure 5 17 Note The default Delta value is 0 05 cm If the tips are a considerable distance from the desired location increase the distance traveled by increasing the Delta value maximum setting is 1 0 cm If the tips are almost to the desired location reduce the Delta value to fine tune the position minimum setting is 0 005 cm 14 Select the directional bu
220. sive ALPs defined 1 8 pin plates installing 2 6 interchangeable 2 6 removing 2 8 storing 2 9 pins interchangeable plates 2 6 replacing 2 9 types 2 6 pod configurations dual pod instrument 1 2 single pod instrument 1 2 framing teaching Multichannel 5 3 Span 8 5 3 manually controlling 6 3 Biomek FX and FX P Laboratory Automation Workstations User s Manual moving to a specific deck position 6 5 Multichannel defined 2 1 4 6 overview 1 7 Span 8 defined 3 1 stopping in Manual Control 6 5 system configuration for 1 2 teaching framing Multichannel 5 3 Span 8 5 3 Pod Editor properties defined 4 9 pod movements for Span 8 Pod 3 4 pod settings deleting 4 36 restoring 4 35 saving 4 34 probes configuring in Hardware Setup 4 12 defined 3 4 identifying 1 thru 8 5 8 pump assembly defined 3 28 pumps defined 3 29 purge settings 4 33 R Reconnect using in Hardware Setup 4 3 S septa fluted tips 3 5 3 7 Span 8 Pod accessibility to deck 3 1 control modes 3 4 current position displayed as coordinates 6 17 defined 3 1 disposable tips 3 20 fixed tips 3 13 labware and tip compatibility 3 10 main components of 3 3 supply container 3 37 Beckman Coulter Inc system fluid 3 37 tubing 3 37 waste container 3 37 movement values defined 6 12 6 19 movements defined 3 4 moving to a safe roving height 6 17 overview 3 1 3 3 performing absolute moves 6 20 performing relative moves 6 18 preventative maintenance 3 53
221. sportation charges collect unless the product is found to be defective in which case the product must be properly decontaminated of any chemical biological or radioactive hazardous material d The Company shall be released from all obligations under all warranties either expressed or implied if any product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair by others is made with the written consent of the Company e Ifthe product is a reagent or the like it is warranted only to conform to the quantity and content and for the period but not in excess of one year stated on the label at the time of delivery It is expressly agreed that the above warranty shall be in lieu of all warranties of fitness and of the warranty of merchantability and that the company shall have no liability for special or consequential damages of any kind or from any cause whatsoever arising out of the manufacture use sale handling repair maintenance or replacement of any of the products sold under the sales agreement Representatives and warranties made by any person including dealers and representatives of the Company which are consistent or in conflict with the terms of this warranty shall not be binding upon the Company unless reduced in writing and approved by an expressly authorized officer of the Company Parts replaced during the warranty period are warranted to the end of the instr
222. sterile up to 1000 uL liquid and air capacity e Disposable P1000 LLS barrier up to 1000 uL liquid and air capacity Note Refer to Section 3 3 Interchangeable Tips for more information on tips available for use with the Span 8 Pod 3 3 2 3 Disabling Clot Detection If clot detection is disabled in the Technique governing the liquid handling procedure clot detection is unavailable regardless of the type of tips attached to the probes Refer to the Biomek Software User s Manual Chapter 10 4 Setting Technique Values for enabling and using clot detection in techniques Beckman Coulter Inc 3 3 3 Span 8 Pod 3 9 Selecting Which Type of Tips to Use The best tips to use depends on a number of factors relating to the application such as the liquid and labware types used and volumes required for aspirate and dispense operations There are 11 types and sizes of fixed and disposable tips that can be attached to the Span 8 probes These tips are listed in Table 3 2 Table 3 2 Span 8 Tip Types Tip Type Tip Name LLS CD Tip Volume Software Capable Fixed 60 Coated Fixed60 Yes maximum of 1000 uL Fixed 60 Uncoated liquid and or air capacity depending on syringe size Fixed 100 Coated Fixed100 Yes maximum of 1000 uL Fixed 100 Uncoated liquid and or air capacity depending on syringe size Septa Piercing Fixed SeptaFluted Yes maximum of 1000 uL gt Pe oe ee available only
223. syringes supported by the Biomek FX are listed in Table 3 4 Note The default Cutoff Velocity value for all techniques and liquid types is the recommended Cutoff Velocity for a 1 mL syringe When 250 uL or 500 uL syringes are installed on the Biomek FX instrument changes must be made to the technique governing the pipetting operation in the Technique Editor and to the parameters of the fluid used during the pipetting operation in the Liquid Type Editor Note The Cutoff Velocities recommended in Table 3 4 are intended as a starting point The values provided should be experimented with in order to determine the most accurate Cutoff Velocity for a specific liquid handling operation Table 3 4 Span 8 Cutoff Velocities for Syringes Syringe Minimum Cutoff Maximum Cutoff Recommended Size Velocity Velocity Cutoff Velocity 250 uL 2 08 uL per second 112 5 uL per second 100 uL per second 500 uL 4 17 uL per second 225 uL per second 150 uL per second 1 mL 8 33 uL per second 450 uL per second 150 uL per second The minimum Cutoff Velocity for each syringe is established using the following equation 50 x lt Syringe size in pL gt 6000 The maximum Cutoff Velocity for each syringe is established using this equation 2700 x lt Syringe size in uL gt 6000 3 4 2 6 Optimizing Syringe Performance and Life Expectancy Syringes should be used cleaned and stored in accordance with the manufacturer s specific
224. t such as a stack of labware Figure 5 21 Warning to make sure gripper are ready for framing Biomek FX and FX P Laboratory Automation Workstations User s Manual 5 24 Framing the Biomek FX 6 In Pick Position Figure 5 22 select a previously framed deck position and frame the gripper to that location The deck position must be framed first and should be a small passive ALP if possible Note Make sure that the AccuFrame is correctly placed on the selected position refer to Section 5 2 3 Installing AccuFrame Pick Position Place the framing tool on a position that has been framed select that position below and hit Ok P5 gt g zE EAE Figure 5 22 Pick previously framed deck position to frame the grippers Beckman Coulter Inc Framing the Biomek FX 5 25 7 Choose OK This moves the pod to that position and extends the grippers Advanced Manual Control Figure 5 23 and Frame Gripper Figure 5 24 appear side by side Note The gripper is extended a little high and opened a little wide at the end of the move initiated by step 6 Advanced Manual Control Pod1 Multichannel Vector Builder Delta ome D Home Z XY y T a Extend Gripper Left a gt Right z for cm 0 1 Retract Gripper BA g D cm am Down m Move Z Max D Units sie lt gt cm aaral Squeeze Aspirate UnSqueeze Dispense C pl r Current Position x o em Y 0 102 om Z 3 95 em D 0 342 cm
225. ter Service Engineer Note The settings for a Multichannel Pod can be saved restored and deleted refer to Section 4 5 Saving Restoring and Deleting Settings 4 3 1 Configuring a New Head When a head on a Multichannel Pod is changed the Hardware Setup must be changed appropriately CAUTION If the hardware configuration is not updated using Hardware Setup hardware crashes or inaccurate liquid transfer may occur CAUTION To avoid hardware crashes a new D axis limit must be established and the gripper framed in the Biomek Software after the head has been configured in Hardware Setup To change the head Note First physically change the head on a Multichannel Pod refer to Section 2 3 2 Changing Heads and then configure Hardware Setup according to the following instructions 1 In Hardware Setup select the appropriate Multichannel Pod Figure 4 4 Note A Multichannel Pod is identified with a 96 or 384 and a Span 8 Pod is identified with an 8 2 Change the Serial Number to correspond to the serial number on the new head Configuring the Biomek FX in Hardware Setup 4 7 3 Choose the appropriate head in Head Type Figure 4 4 Biomek Hardware Setup Reconnect ff Home All Axes Sh Add Device S Remove Device Y Accept gt lt Cancel Bi FX SN N 7 A Biomek Fx ne Serial Number None Save Settings Restore Settinas Delete Settings fa AccuFrame 96 Head Type ES Mandrel 2
226. that the gripper is retracted before homing To home the pods 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Home All Axes The following Warnings and Information appear depending on the type of pods on the system Figure 6 3 Figure 6 4 Figure 6 5 Figure 6 6 and Figure 6 7 lt lt xl Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the grippers on the Multichannel Pod are retracted Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure that the two arms are not near each other at either end of the rail i Cancel CAUTION Before selecting OK to home all axes the following conditions must be met Figure 6 3 Warnings must be addressed before homing process begins l Make sure that the two arms are not near each other by the either end of the rail If they are grab one and move it out of the way Figure 6 4 Warning to address if the system is dual pod You do not have any Span 8 washstations defined You can still prime but you will spill lots of liquid on your deck Hit OK to continue or Cancel to choose a more suitable position Cancel Figure 6 5 Warning to address if a Span 8 Tip Wash ALP has not been added to the deck configuration A Th
227. the Bar Code Reader 000000005 B 22 B 3 5 Aligning the Laser Beatie 5 dc0vecaddeeceent hee heen dd eahhaws B 22 B 4 Configuring the Stacker Carousel in Hardware Setup 0 005 B 25 B 4 1 Installing Stacker Carousels 000 000 c ec eee nee B 25 B42 Configuring Stacker Carousel 1420 00dudVerves idabtocudvars B 27 B 4 3 Removing Stacker Carousels 0 0 00 c cece eee B 28 B 5 Framing the Stacker Carousel 23 iccctjcaieuesebssdeanddeadnea bee eaend B 29 INGO eee ee Ce emer a e eee eee ee eee e xxvii Biomek FX and FX P Laboratory Automation Workstations User s Manual List of Figures Figure 1 1 Biomek FX main COMpPONENES csssccsscosessesenssessonssssssscssacssntenseoncessersseaassansenssens 1 3 Figure 1 2 Main components and connections of the Biomek FX towers eeceseereeeeeeees 1 4 Figure 1 3 Inside and outside tower connections detail view ccescceseeseeeseeceeceteeeeeeeeseees 1 5 Figure 1 4 Bridges move in the X axis and hold and move pods in the Y and Z axes 1 7 Figure 1 5 Protective barriers for the Biomek FX instrument cseeceeseeeeeeeceeceeeeneeeneeeeeees 1 9 Figure 1 6 Replacing fuses in tower escsaosscdsssdsvantcnicnsncpaadessstannsvniesedbactsanccbanvontentasedianensieseabesats 1 14 Figure 2 1 Multichannel Pod installed on Biomek FX sesessessessessssessesrrsesstsresssrrsessesersesse 2 2 Figure 2 2 Multich
228. the Biomek FX instrument This ensures that the pods and gripper locate each position reliably There are three indicator lights on the AccuFrame e First light indicates that the AccuFrame is powered on e Middle light indicates the framing status of the Y axis e Third light indicates the framing status of the X and Z axes Note The AccuFrame is calibrated by a Beckman Coulter Service Engineer The calibration values are stored on the AccuFrame and read as necessary by Biomek Software Beckman Coulter Inc Framing the Biomek FX 5 3 Framing the deck positions of the Biomek FX using AccuFrame is performed in the same manner for either a Multichannel Pod or Span 8 Pod except a framing fixture is attached to the head of a Multichannel Pod while a framing shaft is attached to a probe of a Span 8 Pod Note When framing multiple position ALPs such as the 1 X 3 Passive ALP all positions on the ALP should be framed to increase precision To frame the deck positions of the Biomek FX the following operations must be completed Homing All Axes of the Pods Section 5 2 1 e Attaching the Framing Fixture to the Pod Section 5 2 2 e Installing AccuFrame Section 5 2 3 Framing the Position Section 5 2 4 Note Some ALPs require additional or slightly modified procedures to frame properly Consult the ALPs User s Manual for the specific ALP to frame for any special instructions Note Deck posit
229. the case of any other framing related problems contact a Beckman Coulter Service Engineer Table 5 1 Troubleshooting Framing If AccuFrame power light not on Then Check the CAN connection to make sure the AccuFrame is connected to Biomek FX instrument The Y axis and the X Z axes Light Beams cannot be broken when moving a finger around the interior of AccuFrame Make sure the AccuFrame is receiving power One or both beam indicator lights on AccuFrame stay on when no objects are breaking the light beams There is most likely an internal obstruction to sensors Call a Beckman Coulter Service Engineer Beckman Coulter Inc Manually Controlling the Biomek FX in Biomek Software 6 1 Overview Manual Control and Advanced Manual Control are used to control e Movement of the bridge head and gripper independently of a method Note Many activities performed in Advanced Manual Control are the same for the Multichannel Pod and the Span 8 however the dialogs are different and the buttons for performing these activities may be placed in different areas e Pod when teaching the deck framing the grippers and recovering from errors e HDR Pin Drying Tip Loader Magnetic Bead Microplate Shaking Positive Position Orbital Shaker and Stirring ALP and the Speed Pump independently of a method Note Refer to the ALPs User s Manual for information on manually controlling specific ALPs
230. the eight pumps in the pump assembly Figure 3 21 and Figure 3 22 Syringes provide pipetting accuracy by controlling the amount of system fluid aspirated from and dispensed into the tubing for each of the eight Span 8 probes The volume of fluid aspirated and dispensed by the syringes is equivalent to the volume the probes aspirate and dispense Beckman Coulter Inc Span 8 Pod 3 31 3 4 2 1 How Syringes Affect Pipetting Accuracy Syringes affect pipetting accuracy by controlling the amount of system fluid aspirated from and dispensed into the tubing for each of the eight Span 8 probes Note The volume of fluid aspirated into and dispensed from the Span 8 tubing is approximately equivalent to the volume of fluid a tip aspirates and dispenses maintaining a minimum and maximum operational speed which dictates how fast fluid can be aspirated into and dispensed from the syringes and ultimately the tips e controlling the amount of fluid dispensed per second by a syringe before the dispense action of the syringe abruptly stops Note The speed at which fluid is dispensed per second prior to the dispense action abruptly stopping is called the Cutoff Velocity If the Cutoff Velocity in the technique or liquid type governing the pipetting operation is too slow droplets can be left at the end of tips after a dispense pipetting operation rather than all the liquid dispensing into the labware 3 4 2 2 Syringe Size The size of
231. tion 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Multichannel Pod appears Figure 6 9 4 Choose Clear to change the Movement Vector values to 0 5 Enter the desired X Y Z and D values in Movement Vector OR Set the Delta values as desired refer to Section 6 4 3 1 Setting Delta Values for the Multichannel Pod and click the appropriate buttons on Vector Builder refer to Section 6 4 3 2 Understanding and Using the Vector Builder for the Multichannel Pod until the desired values appear in Movement Vector Note Hold the button down to quickly add Delta value to the Movement Vector Note Positive values move the pod to the right X toward the front of the deck Y up Z and squeeze aspirate D Negative values move the pod to the left X towards the back of the deck Y down Z and unsqueeze dispense D Note Minimum and Maximum X Y Z and D values displayed in Hardware Setup indicate how far the pod is able to move These values are also displayed as a tool tip when hovering over the fields in Movement Vectors 6 Enter a value in Speed to specify the percent of the pod s maximum speed 7 Choose Go The pod moves from its current position to a new position by the values displayed in the Movement Vector The new position is displayed in Current Position 8 Choose Close to cl
232. tip portion should descend into 10mm test tubes tip shuck tubes should not descend into 10mm test tubes The clearance between the tip shuck tubes and a 10mm test tubes is too tight to safely allow the tip shuck tubes to access 10mm test tubes Maximum diameter Accessibility of disposable tips into a 10mm test tube is limited to the height of the tip Tip height 10mm test tube Figure 3 5 Disposable tip and tip shuck tube width restrictions with 10mm test tubes 3 3 4 Electro Static Discharge Protection when Changing Tips on the Span 8 Pod CAUTION To prevent damage due to electrical static discharge ESD AN wear a wrist ground strap when working with tips on the Span 8 Pod A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tips on the Span 8 Pod or moving the Span 8 Pod by hand Note A wrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek FX instrument To attach a wrist ground strap 1 Turn off the power to the Biomek FX instrument 2 Position the strap portion of the wrist ground strap snugly around the wrist Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 14 Span 8 Pod gt amp Beckman Coulter Inc 3 Clamp the alligator clip of the wrist ground strap
233. to the instrument deck Figure 3 6 4 Power up the Biomek FX instrument Strap portion of wrist ground strap snugly attached to the wrist Alligator clip clamped to the instrument deck base plate Figure 3 6 Wrist ground strap attached to the deck 3 3 5 Fixed Tips All fixed tips are LLS capable when liquid level sensing is enabled in the technique governing the liquid handling procedure If liquid level sensing is disabled in the technique LLS is unavailable regardless of the type of tips attached to the probes Refer to Section 6 5 6 Verifying Liquid Level Sensing for more information on detecting the liquid level in labware Refer to the Biomek Software User s Manual Section 10 4 Setting Technique Values for enabling and using liquid level sensing in techniques To use fixed tips they must be physically installed on the probes and configured for use in Hardware Setup refer to Chapter 4 4 Configuring a Span 8 Pod Note After installing fixed tips it is necessary to frame the Span 8 Pod 3 3 5 1 Installing Fixed Tips CAUTION Do not perform aspirate or dispense operations without mandrels installed and tubing attached to fixed tips To operate the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface CAUTION To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 P
234. tries to 0 Current Position Current location of the pod after the pod has been homed Delta Sets the magnitude of change the Vector Builder applies to the Movement Vector for each axis Get Tip Status Indicates which probes have tips Home Z XY Moves Z then X and Y to the home positions LLS Z Move Verifies liquid level sensing by allowing probes to descend and then stop once they hit liquid Move Z Max Moves pod to highest configured height Movement Vector The amount of movement that occurs when Go is selected Movement vectors are relative to the current position Purge Syringes Removes air from the tubing and syringes and ensures lines are filled with system fluid Refresh Updates the current position of the pod after it has been physically moved without using Manual Control Set Valve States Sets valves on the pumps to allow system fluid to fill empty or bypass the syringes Speed Sets the speed of the pod with 0 percent meaning use current speed Vector Builder Relative moves are created using the Vector Builder Each time a Vector Builder button is pressed the Movement Vector is changed in the corresponding axis by the amount indicated in the Delta box Up and Down move the pod in the Z axis Left and Right move the pod in the X axis and Back and Fwd move the pod in the Y axis Widen Narrow change the Span of the probes Aspirate Dispen
235. tton representing the motion required to physically move the tip into position over the wells of the microplate on top of the ALP Figure 5 17 Note Each time a directional button is selected the pod and tips move the distance specified in Delta in the indicated direction Note The tips can be can physically positioned over the wells of a microplate using e the directional buttons in Manual Teach e the directional keys on the keyboard e the directional keys on the numeric keypad The directional buttons displayed in Manual Teach parallel the keys on the numeric keypad More specifically Fwd correlates to the 1 on the numeric keypad while Down is found on the 2 Left is found on the 4 Right on 6 Up on 8 and Back on 9 OR Using the graphic alignment tool Figure 5 17 drag the center small circle until it represents the tip s physical position in relation to the wells of the microplate on top of the ALP Note The small circle represents the tips on the pod The objective is to provide the software with a representation of the tip s position in relation to the wells of the microplate on top of the ALP The software uses this graphical representation to know approximately how far in any direction the tips must move 15 Select Go The pod moves in accordance with the position of the small circle in relation to the large circle Note When the move is completed the small circle reset
236. ument warranty Note Performance characteristics and specifications are only warranted when Beckman Coulter replacement parts are used Biomek FX and FX P Laboratory Automation Workstations User s Manual Safety Information All Warnings and Cautions in this document include an exclamation point a lightning bolt or a light burst symbol framed within a triangle Please pay special attention to the specific safety information associated with these symbols WARNING If the equipment is used in a manner not specified by UN Beckman Coulter Inc the protection provided by the equipment may be impaired Warning and Caution Definitions The exclamation point symbol is an international symbol which serves as a reminder that all safety instructions should be read and understood before installation use maintenance and servicing is attempted When this symbol is displayed in this manual pay special attention to the specific safety information associated with the symbol WARNING A WARNING calls attention to a condition or possible situation that could cause injury to the operator CAUTION A CAUTION calls attention to a condition or possible situation that could damage or destroy the product or the operator s work Biomek FX and FX P Laboratory Automation Workstations User s Manual vi Safety Information Electrical Safety Laser Light A Beckman Coulter Inc To prevent electrically related injuries a
237. ure 3 35 5 Slide the ferrule from the vacant port on the tubing connector up approximately 1 from the end of the tubing projecting from the distribution manifold at the back of the pump assembly Figure 3 34 6 Slide the end of the tubing with the ferrule over the vacant port on the tubing connector Figure 3 35 Note Make sure the tubing is securely seated to the port on the tubing connector Note When tubing is repeatedly removed and reattached the end of the tubing may stretch out or split If tubing is not forming a tight fit or if tubing is damaged cut off approximately 1 2 of tubing to remove the damaged portion before attaching the tubing to the port 7 Slide the ferrule down onto the port 8 Tightly screw the ferrule onto the port by turning the screw to the right Visually verify that it is fully seated Figure 3 35 Attached tubing and ferrule The tubing and ferrule i are positioned so they are flush with the base of the port Tubing connector Tubing port Ferrule Figure 3 35 Tubing and ferrule attachment to the tubing connector Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 52 Span 8 Pod 9 Attach the tubing connector to the back of the Speed Pump by squeezing the tabs on each side of the connector and pushing it firmly onto the ports on the back of the Speed Pump Figure 3 36 The tabs s
238. urning the blank 180 in the appropriate direction Beckman Coulter Inc Using a Stacker Carousel B 9 Right side shield with cutout blanked Right side shield with front cutout blank Right side shield with rear cutout blank Use for deck position P2 or P18 Use for deck position P1 or P17 Figure B 9 Plexiglas side shield cutout blank orientations 2 Following this diagram in Figure B 10 attach the cutout blank to the Plexiglas shield Biomek FX and FX P Laboratory Automation Workstations User s Manual B 10 Using a Stacker Carousel 3 Attach the shield to the Biomek FX Note Use the same attachment procedure for either side of the instrument 719318 Biomek FX right tower side 804514 7 810590 5 Ss 1719296 717710 804409 3 864678 5 804491 6 Biomek FX right front rail end Figure B 10 Diagram for attaching blank to shield and shield to Biomek FX Beckman Coulter Inc Using a Stacker Carousel B 11 4 Align the shuttle to load and dispense labware through the Plexiglas side panel opening Figure B 11 and Figure B 12 Side shield fasteners Blanking plate rear or
239. ut of the liquid there is a large change in capacitance detected A clot is detected by determining the change in capacitance as the tip moves up after aspiration 3 3 2 1 Clot Detection Settings Sensitivity settings for clot detection sensing affect the magnitude of change required for conductive tips to detect a clot A smaller sensitivity setting detects smaller changes in the capacitance refer to the Biomek Software User s Manual Chapter 10 4 Setting Technique Values 3 3 2 2 Clot Detecting Tips WARNING Use care when handling septa fluted tips they are extremely sharp CAUTION Use care when handling septa fluted tips they are extremely fragile gt amp Biomek FX Span 8 supports the following clot detecting tips e Fixed 60 Coated and Uncoated maximum of 1000 uL liquid and or air capacity e Fixed 100 Coated and Uncoated maximum of 1000 uL liquid and or air capacity Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 8 Span 8 Pod e Septa Fluted maximum of 1000 uL liquid and or air capacity Note Fixed and septum piercing tips can pull samples into the tubing but the maximum sample volume is limited to syringe capacity refer to Section 3 4 2 Syringes e Disposable 20uL LLS up to 100 uL liquid capacity up to 120 uL air capacity e Disposable 200uL LLS up to 220 uL liquid capacity up to 240 uL air capacity e Disposable P1000 LLS sterile and non
240. ve to allow probes to descend and then stop once they hit liquid 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Span 8 Pod appears 4 Check LLS Z Move 5 In Movement Vector enter the maximum Z distance each probe should descend Note Use Active Axes Figure 6 12 to disable any probes that should not descend Checkmarks next to the probe number indicate the probe will descend when Go is chosen Choosing All selects all the probes choosing None selects none of the probes 6 Choose Go 7 Choose Close to close Advanced Manual Control 8 Choose Exit to close Manual Control 6 5 7 Purging Air from the Syringes and Tubing To accurately transfer liquid using a Span 8 Pod air must be purged from the syringes and tubing Note It is necessary to purge air from the syringes and tubing before running all methods To purge air from the syringes and tubing 1 Choose Instrument gt Manual Control Manual Control appears Figure 6 2 2 Choose Advanced Controls 3 Select Pod1 or Pod2 Advanced Manual Control for the selected Span 8 Pod appears 4 Choose Purge Syringes The following warning appears if a Span 8 Tip Wash Station is not defined Figure 6 16 You do not have any Span 8 washstations defined You can still prime but you will spill lots of liquid on your deck Hit DK to continu
241. waste container Pump Waste Connection Waste from the Span 8 pump assembly overflow terminates in the waste container Waste from the 3 8 Tubing pumps is a safety overflow in case of lt Cap leaks in the tubing attachments 4 Quick Disconnect Fittings Figure 3 31 Waste container 3 5 4 1 Replacing a Waste Container The waste container attaches to the Biomek FX instrument via quick disconnect fittings and 3 8 tubing on its cap To replace a waste container 1 Detach the pump and Span 8 Tip Wash ALP connections by pressing in and pulling up on the tabs on the quick disconnect fittings Figure 3 31 2 Unscrew the cap on the waste container by turning the cap counterclockwise 3 Lift the cap straight up and off the bottle 4 Remove the full waste container and dispose of as directed by the laboratory safety officer Note Waste containers can be reused however waste materials contained in the waste container must be disposed of as directed by the laboratory safety officer 5 Position a new waste container in the same location the original waste container occupied Note The waste container is positioned at installation to minimize tubing length to keep kinks and bends out of the tubing and to reduce uphill slopes as much as possible Positioning the new waste container in the same location ensures proper alignment of the tubing and max
242. xed Disposable Conductive Disposable tips Stainless Steel or Teflon Coated Stainless Steel Polypropylene Polypropylene Impregnated with Carbon Disposable tip mandrel Stainless Steel Note All fitting threads may be wrapped with Teflon tape Biomek FX and FX P Laboratory Automation Workstations User s Manual 3 40 Span 8 Pod 3 5 1 1 2 Parts Along the Wash Station System Fluid Flow Path Since system fluid may be used as a wash fluid each of the Span 8 Tip Wash ALP parts that comes into contact with the system fluid or waste fluid must also be considered for possible susceptibility to the caustic nature of non supported system fluid and is therefore listed in Table 3 6 Table 3 6 Parts and materials along the Span 8 Tip Wash ALP fluid flow path Part Material 8 Channel Passive Wash ALP Polypropylene Outlet Fitting Polypropylene Tubing Tygon 2075 Quick Disconnect insert Polypropylene EPDM and Stainless Steel Quick Disconnect body Polypropylene EPDM and Stainless Steel Tubing Tygon 2075 Cap Polypropylene Carboy Polypropylene Note All fitting threads may be wrapped with Teflon tape 3 5 2 Tubing The tubing is a means of transporting system fluid to and from the Span 8 Pod The Span 8 Pod uses 3 8 tubing from the supply container to the pumps 1 8 tubing from the pumps to the probes of the Span 8 Pod and 1 4 tubing from the pump assembli

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