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CO2 Incubator HERAcell 150 i / 240 i with Decontamination Routine

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1. 4 4445545555 4 4 enne 94 Event error 8 6 95 Decontamination routine phases c cccceceeeeeeeeeeeeeaeeaeeeeeeeeeeeeeaeaees 106 Activating Contra GOM caaan anaana aana a aaa EEE 107 Interrupting Cancelling contra con eese 108 contra con COMPIetiON eeececeeecee cece eee eeeeee eee ee eene 109 Preparing the temperature calibration 5 44 545555555559559999 85 855656 111 Temperature calibration procedure 555554 4544555555 55 54 44949 5485385556 112 Preparing CO calibration 5 4 4355 4435 4444944459 113 Gas tight screen measurement 1 6 555559455555559 55559 114 CO calibration procedure ssssseeeeeeeneen nennen 115 Gas inlet filter installation esses esee 116 Device fuse replacement essen enne nnns 116 Magnetic door seal replacement essssseeseeeeenee 117 Operating Instructions HERAcell 150 i 240 i hermo 1 General notes Overview of the Thermo Fisher international sales organisations Europe Middle East Africa Postal address Germany Thermo Electron LED GmbH Robert Bosch Straf3e 1 D 63505 Langenselbold Enquiries from
2. Act val gt nom val 1 Check gas supply Reduce x 4 prepressure to 1 bar max Check gas supply Change Act val nom val 196 gas cylinder Raise 4 prepressure to 1 bar max Check supply line to device Calibration values Max adjustment value too high too low exceeded for CO Failure Measuring cell cannot nm Contact Service x x communication commuicate with mainboard Failure gas Gas cylinder changeover cylinder switch cannot commuicate Contact Service x x changeover switch with mainboard Replace one or both O2 N Both CO2 cylind t Gas cylinder A r G linder A ty Repl linder A 6 Gescyinderaisempiy Gas cylinder 8 G linder B ty Repl linder B me et cosevinaersizemory Repco gasovindors sensor Detected value is out of Act val gt nom val 1 Check gas supply Reduce Actual value above g PPY x x 4 prepressure to 1 bar max Actual value below Check gas supply Change io Ausus vuelo Act val nom val 196 gas cylinder Raise i 4 prepressure to 1 bar max Check supply line to device Failure O sensor cannot Contact Service x x communication commuicate with mainboard 4 After changing the set value error time will be set on 159 min after door opening on 45 min O on 159 min 50115191 07 2008 97 Operating Instructions HERAcell 150 i 240 hermo 7
3. inser for pressure compensation opening 9 Oo 2 oo Pugforphecame Aleswenhimmfebowerwwd O 1 1 Allen wrench3 mm forblowerwhesteover 1 3 Opwwmomsmeios D 1 2 Ourense 2 4 Optional equipment components HERAcell 150 i HERAcell 150i Quantity of the delivered components CO incubator or CO O incubator in piece Solid glass door and 3 door gas tight screen and continuous shelves continuous shelves Lo owedmghsess O 1 Oz sensor with set for gas humidan y 3o o 1 CO connecting hose set gas monitoring optional 7 oo o o O Ne connecting hose set gas monitoring optiona 1 1 1 02 sensor with gas humidification set is packed in a separate carton and placed into the work space during transport 50115191 07 2008 19 Operating Instructions HERAcell 150 2401 i hermo 2 5 Standard equipment components HERAcell 240 i HERAcell 240i CO incubat CO O incubat Quantity of the delivered COS Solid glass door and Solid glass door 6 door gas tight 6 door gas tight continuous shelves and split shelves screen and screen and split standard version optional continuous shelves shelves standard version optional Shelf supports for shelf
4. 555555 59 04949455455345555555659599494 8946 1 3 Applicability of the instructions cesses nnns I AEn 1 5 Explanation of safety information and symbols sssseseesees 1 5 1 Safety information and symbols used in the operating instructions 1 5 2 Additional symbols for safety informations sssssesseeee 1 5 3 Symbols on the device ssssssssseeeeenenenenne nennen nennen 1 6 Intended purpose of the device ssssssssssesseeeeeeeeenn nnne 1 6 1 Correct use cee ceeeeeeeee cece cette eee tae ee nnn eee nnne 1 6 2 Incorrect Se eite den ia rene eu VER ERR IA RSS RE TRERS 1 7 Standards and directives 44444 8 nennen nnns 1 8 Safety notes on 68568 an aa nennen ns 1 8 1 Safety notes on carbon dioxide COd 445 54949455555999555599 1 8 2 Safety notes on oxygen O5 siirsin daanan enne 1 8 3 Safety notes ON nitrogen 44445 4435 nenne 2 Delivery of the device 2 1 PACKAGING eis ocsnscude EE 2 2 Acceptance 5 6 0 cee c
5. 48 nennen nnns 49 5 2 1 Installation removal of the support rail 4 4444445444 49 99 999 5555566 49 5 2 2 Installing the shelf supports 2 0 0 cee eeeeeeeeee eae teen nennen 50 5 2 3 Levelling the device 4 5 5 9 5 555 nnns 50 5 3 Installing the gas humidification optional only for O2 N5 control 50 5 4 Installing the optional bottle turning devices HERAcell 240 i 51 5 5 Installing the optional center strut HERAcell 240 i for split inserts 54 5 6 Gas cORFPectiOn iei e xS RR RR ERR NER IER QU UNA EN IS teeta 55 5 6 1 Installing gas pressure NOSES 0 0 cece cece 44 cette teen teen 44 44 4888585866 55 5 6 2 Installing devices without the optional gas monitoring system 56 5 6 3 CO connection with gas monitoring system optional 57 5 6 4 Combined CO4 O5 N connection with gas monitoring system optional 57 5 7 Power supply connection sssssssssssseessesee eene 4 nnne 59 5 8 Connecting the RS 232 interface 55 55 5444444535 eene 60 5 9 Connecting the USB interface optional sese 60 5
6. Calibration level 2 bytes old 0x41 RSE eee CO value new CO value 2 calibration bytes each Calibration level 2 bytes old 0x42 S se O value new O value 2 bytes calibration each 0x50 Status error registration for a cal temperature CO O and rh 0x51 auto start successfully Current actual values for completed temperature 053 O and rh 50115191 07 2008 140 Operating Instructions HERAcell Bit coded event entries 50115191 07 2008 150 i 240 i 0x90 Overview of event entries Part Il auto start completed with error auto start stopped manually Start contra con contra con successfully completed contra con completed with error contra con stopped manually Gas monitor cylinder A empty Gas monitor cylinder B empty Gas monitor manual changeover Start low humidity Stop low humidity Start bottle turning device Stop bottle turning device Delete data logger Last entry of data logger 141 Special information byte 8 15 Status error registration for temperature COs O and rh Status error registration for temperature CO O and rh Status error registration for temperature CO 232 and rh Current actual values for temperature CO 232 and rh Status error registration for temperature CO O and rh Status error registration for temperature CO O and rh Status gas monitoring 2 bytes
7. 7 Fig 25 Gas connection with gas monitoring system optional Operating Instructions HERAcell 150i 240i connection of the gas monitoring system connect the gas container A 3 to the lower connection of the gas monitoring system The outlet of the gas monitoring system 4 is connected at the factory to the gas inlet filter 9 by a short gas pressure hose 11 CO supply e Distributor connection 5 for the CO gas supply for up to three additional devices For looping the gas supply through interconnect the devices with a gas pressure hose Connect the gas container B to the upper connection 6 of the gas monitoring system connect the gas container A to the lower connection 7 of the gas monitoring system The outlet of the gas monitoring system 8 is connected at the factory to the gas inlet filter 10 by a short gas pressure hose 12 NOTE Looping the gas supply through By looping the gas supply through up to 4 devices see annex at the end of this section cyn be supplied by a CO gas supply or O5 N5 gas supply 50115191 07 2008 58 Fig 26 Combined connection with gas monitoring system optional Operating Instructions HERAcell 150i 240 li hermo 5 7 Power supply connection Electric shock Contact with current carrying components may cause a lethal electric shock Before connecting the device to the power
8. 4 5 4544444449 72 38 Activating the auto start cee ceseee eee 4 4 94994 4 4 5 75 39 Interrupting the auto start 5 cee cece ceteeee cece eee 4 944 4 76 40 Changing the code 444 nennen 77 41 Date time setting cece cece eect e eee ete 484 nnn nnnrnn nnne nnn nn 78 42 Display contrast setting 5 5 555555 4445 nennen nennen 79 43 Key tone setting 5555554 44 etree eae nnne nnne sten nn nnn 79 44 RS 232 interface baud rate setting 50 55555555555505540999455 55555556595594999 80 45 USB interface baud rate Setting eeeeeeeeeeneee eee ee eee 4 eee eeaeaeeeeeeeeeeeeenaaae 80 46 banguage Settlng o ne E Rire diea se 8 aE 81 47 Reminder interval setting ssssssssseeseseeennenenennene nnne 82 48 Eventdisplay erret DR pax the seen const 83 49 Logging cycle setting sssssssssesseeseeeeeen ennemi 83 Operating Instructions HERAcell 150i 240 i 50115191 07 2008 Fig Fig
9. naana n enn 76 TMT 211015 EE 77 7 11 2 Event LOGGING roc 83 AIRS Xe 85 FANE Icon descriptio Minssen eaa etna 90 7 11 5 Enabling disabling the keypad 06 5 5 55 55595555555555559599 99955 92 7 11 6 Software Vers ONS croci ena ct ec argu RATER EPA 93 7 12 Scaling the trend display ssssssssssssseseeeenenenen enne 94 7 19 ErrOF MESSAGES rer Re 95 7 13 1 Response to an error message event sssssssseeeeeenne 95 7 13 2 Troubleshooting ier tote 95 Operating Instructions HERAcell 150 i 240 i 50115191 07 2008 8 Shut down 99 8 1 Shutting the device down sss eene nnns nnns nana nnana 99 9 Cleaning and disinfection 100 9 1 Cleanings saps 100 9 2 Decontamimation ProCe CUures 44433 nnn nnns 101 9 3 Wipe spray disinfection ee nennen nnns 101 9 4 contra con decontamination 1 4 eee 4 nennen 104 9 4 1 Activating the contra
10. FL 30 40 50 60 70 Adjustment ranges of the roller drives in Fig 21 Bottle turning speed 50115191 07 2008 53 Operating Instructions HERAcell 150i 240 5 5 Installing the optional center strut HERAcell 240 i for split inserts If HERAcell 240 i is equipped with the optional 6 segment insert two center struts 1 with perforations at each side must be installed In this case the shelf support rails must be installed to the support rails at the sides and at the left and right side of the strut The support rails marked with the diamond shape are installed at the rear panel of the device 1 A pressure spring keeps the upper rectangular guide 3 of the center strut under tension Insert this guide into the receptacle 2 in the work space ceiling und push it slightly upward so that it is pushed into the center strut 2 Then position the lower rod guide 5 of the center strut into the hole 6 in the work space floor and remove spring tension 3 The center strut is stabilized by the spring pressure NOTE Configuration with split inserts For versions with split inserts bottle turning devices cannot be installed 50115191 07 2008 54 Fig 22 Center strut installation Operating Instructions HERAcell 1501 240i li he
11. gt The warning triangle 3 is displayed as a failure message together with the audible signal 3 To accept the failure message gt Press any position on the display gt The ERROR dialog box with the description of the error is displayed 4 To end the auto start routine gt Press the END key 4 gt The system returns to the main menu 7 10 Running the contra con routine contra con is an automatic decontamination routine for disinfecting the device work space For a detailed description of this device function see chap 9 4 pg 104 7 11 User configuration The user configuration settings allow the user interface and the additional device functions to be adjusted to the requirements of everyday operation The user configuration menu is split into six categories Settings Event logging Options con description 50115191 07 2008 00 xxx 2008 00 00 HERAcell 150 i 240 i auto start stop lt End gt Stop the auto start routine lt Back gt Continue HERAcell 150 i 240 i 00 xxx 2008 00 00 HERAcell 150 i 240 i 00 xxx 2008 00 00 Error Fig 39 Interrupting the auto start routine Operating Instructions HERAcell 150 240 i li hermo 7 Handling and control Keypad lock Menu Software versions gt Settings To make a user specific setting in a dialog box gt Keypad lock code navigate through the submenus listed in the illustrations an
12. Shelf supports for shelf for shelf Insert for pressure compensation opening Plugfor pipe channel Plugfor pipe channel pipe channel components in piece Powersupply cable Powersupply cable cable Connector potential free eee ol Spare caps set caps set Open end wrench 24 mm Open end wrench 24 mm wrench 24 mm Allen wrench 2 mm for blower wheel Allen wrench 3 mm for blower wheel cover 1 instructions CD __ E RR EE 50115191 07 2008 20 Operating Instructions HERAcell 150 2401 i hermo SCIENTIFIC 2 6 Optional equipment components HERAcell 240 i HERAcell 240i Quantity of the delivered CO incubator or CO O incubator components Solid glass door and Solid glass door 6 door gas tight 6 door gas tight in piece continuous shelves and split shelves screen and screen and split standard version optional continuous shelves shelves standard version optional 03 connecting 1 1 1 hose set O gas humidification system connecting hose set 1 Bottle turning device motor with four rollers optional 2 3 CO connecting hose set gas 0 optional ERO 111 gas monitoring optional 1 O sensor with gas humidification set is packed in a separate box that is placed into the work space during transport 2 For bottle turning devices the version with split shelves is not available 3 Each level of a bottl
13. the water level sensor can be switched off This prevents alarm messages for the water level sensor by the device integral monitoring system from being issued 1 To toggle between two states 1 gt Press the key 4 or gt Press the key 2 2 To accept and save the change 50115191 07 2008 87 Menu gt Options gt Gas tight screen HERAcell 150 i 240 i 00 xxx 2008 00 00 D Gas tight screen current Glass door a nw Gas screen SI ES 2 3 4 Fig 53 Gas tight screen setting Menu gt Options gt Water level sensor HERAcell 150 i 240 i 00 xxx 2008 00 00 8 Water level sensor current off new 2 3 4 Fig 54 Water level setting Operating Instructions HERAcell 150i 240 7 Handling and control gt Press the ENTER key 3 gt The system returns to the Options menu Switching the audible alarm on off If the device integral monitoring system detects an error e an audible alarm sounds e in addition to the visual error message and the switching of the alarm relay The audible alarm can be permanently disabled 1 To toggle between two states 1 gt Press the key 4 or gt Press the key 2 2 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Options menu Setting the bottle turning device speed option available only on HERAcell 24
14. 4 nennen nnn 54 23 Installing gas pressure NOSES sese 55 24 Gas connection without gas monitoring system sues 56 25 Gas connection with gas monitoring system optional 57 26 Combined connection with gas monitoring system optional 58 27 Combined gas connection with gas monitoring system 59 27 Power supply connection cece cette ee 4 cece eset eee ee eene nnne nnn 59 28 Connection example teeta aaa eae eee eee eene 62 29 Power SWITCH eR RURIBRMI 65 30 Pressure sensitive areas ence ae eae ee eee ee nnne nnn 65 31 Operating panel withoutO gt Nogas supply eee eene 66 32 Operating panel with combined gas 9 9955 66 33 Menu structure nennen nennen nnne nnn 67 34 Heat up phase display 4 nnne nnne 68 35 Setting the temperature set value cceceeeee cette 49 95494 998 70 36 Setting the CO set ValUC cece 4444444444444444 444444 554 5 71 37 Setting the 32 set value 4 eee
15. Keep these operating instructions close to the device so that safety instructions and important information are always accessible Should you encounter problems that are not detailed adequately in these operating instructions please contact Thermo Scientific immediately for your own safety 1 4 Warranty Thermo Scientific warrants the operational safety and functions of the CO incubator only under the condition that the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions the device is not modified only original spare parts and accessories that have been approved by Thermo Scientific are used inspections and maintenance are performed at the specified intervals The warranty is valid from the date of delivery of the device to the operator 11 Operating Instructions HERAcell 150 i 240 i hermo 1 General notes 1 5 Explanation of safety information and symbols 1 5 1 Safety information and symbols used in the operating instructions WARNING Personal injuries Indicates a hazardous situation which if not avoided could result in death or serious injuries CAUTION Personal injuries Indicates a hazardous situation which if not avoided could result in minor or moderate injuries NOTICE Property damage Indicates a situation which if not avoided could result in property damage NOTE Useful information NOTE Is used for a
16. control is switched off or the O5 sensor is disabled 7 11 4 Icon description Essential operating states or error messages e g keypad lock 3 low humidity 4 or bottle turning device 5 are displayed as icons in the touchscreen main menu in addition to the entries in the event or in the error table The Icon Description dialog box 1 explains the meaning of the individual icons To exit the display gt Press the END key 2 gt The system returns to the Configuration menu Function of the individual icons Bottle turning device This function indicator shows that the device has been configured for operation with the optional bottle turning device The Bottle Turning Device dialog box can also be accessed directly by touching the Bottle Turning Device icon in the main menu Low water level This error indicator shows that the water level sensor has detected a low water level Low humidity This function indicator shows that the relative humidity in the work space has been lowered from approx 93 to approx 90 Keypad lock This function indicator shows that the keypad lock has been activated so that currently the settings cannot be changed Overtemperature This error indicator shows that the device control has activated the overtemperature protection and has switched to backup control 50115191 07 2008 Menu gt Icon discription HERAcell 150 i 240 i 00 xxx 2008 00 00 Bottle turning
17. start time NOTE Cancellation The auto start routine can be cancelled at any time Press the Stop key 6 Automatic restart The auto start routine is restarted automatically if the routine is cancelled due to one of the following events The glass door is opened 50115191 07 2008 75 HERAcell 150i 240 i 00 xxx 2008 00 00 Temp 36 9 p 4 9 x HERAcell 150 i 240 i auto start instru 1 Press lt Start gt 2 Open door until buzzer sounds 30s 3 Fill water if necessary 4 Close door Duration max 10 hours C A 3 4 HERAcell 150 i 240 i 00 xxx 2008 00 00 auto start status 36 8 C Start time J Stop Temperature 13 10 6 Fig 38 Activating the auto start routine Operating Instructions HERAcell 150i 240 7 Handling and control the outer door is opened on a device with optional gas tight screen the power supply is interrupted 7 9 2 Interrupting the auto start routine If the STOP key in the status display is depressed see above the auto start routine is interrupted and the auto start stop dialog box is displayed for a safety scan The routine can now be permanently cancelled or resumed 1 To resume the auto start routine gt Press the BACK key 1 gt The system returns to the status display and the auto start routine is resumed 2 To cancel the auto start routine gt Press the END key 2
18. 10 1 N PE 100 V AC 10 Tnteerence suppression BN VDE S f m DWewodms OwwamecwegyENemU 2 5 230 V AC Rated current A 5 2 120 V AC 4 3 100 V AC On site fusing Fuse Circuit breaker kW 0 58 230 VAC Rated input kW 0 62 120 VAC kW 0 43 100 VAC 50115191 07 2008 121 Operating Instructions HERAcell HERAcell 240 i Ve 780 x 934 x 834 607 x 670 x 583 approx 238 560 x 500 150 i 240 i Thermo External dimensions W x H x T Interior dimensions W x H x T Chamber volume Shelves W x T Standard quantity Maximal quantity Maximal surface load o W u o N 10 insertion shelf Maximal device overall load Weight without accessories Thermal Class 3 1 overtemperature controller TWW with overtemperature detection function C 18 33 C RT 3 55 c Thermal safety devices according to DIN 12880 2007 05 Ambient temperature range Temperature control range Temperature deviation time DIN 12880 Part 2 Temperature deviation spatial DIN 12880 Part 2 at 37 C 1 Duration of the au
19. 135 15 5 3 Overview of the possible error messages in bit coding 136 15 6 Data logger stru ctu re erit EA RA RIA 138 15 6 1 Data logger data set structure scheme ccccceccececeeeceeceeeceeeeeeeeeeeeeananaes 139 15 6 2 Overview of possible event entries in bit coding 9 9 140 15 7 Examples of data logger codes nennen 142 15 7 1 Functions for data logger queries 9 555555959 5555599 95555599 85556699 5 142 15 7 2 Example of a code for a a data logger query 142 15 8 Program Heracell 150 i amp 240 5555555555559595949545 5555559699499 8 454656 147 15 8 1 Installing the Heracell 150 i amp 240 i 148 15 8 2 Operating the Heracell 150 i amp 240 i sss 149 50115191 07 2008 6 Operating Instructions HERAcell 150 i 240 i 50115191 07 2008 Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 1 Device 810 nnnm nennt nnn nennen 23 MEE Eigene ete 24 Bi cidem E
20. 240 i 00 xxx 2008 00 00 Temp change set value 8 37 0 current a T 37 0 4 5 6 Fig 35 Setting the temperature set value 50115191 07 2008 70 Operating Instructions HERAcell 150i 240 7 Handling and control 7 7 Setting the CO set value 1 Press the CO DISPLAY key 1 gt The CO menu is displayed 2 To exit the CO menu gt Press the END key 2 3 To set the CO set value gt Press the SET VALUE key 3 To increase the set value gt Press the key 6 To reduce the set value gt Press the key 4 4 To accept and save the set value gt Press the ENTER key 5 gt The system returns to the main menu The CO3 display shows the actual value currently measured in the work space NOTE Deactivating the CO control loop To deactivate the CO control the set value is set to 0 If the control loop is deactivated error monitoring is enabled as well Gas monitoring The switching state of the CO control loop does not affect the function of the optional gas monitoring system The gas monitoring system remains active even when the CO control is switched off For set values of 21 O5 the 32 control is switched off and error monitoring is disabled as well The control loop error monitoring system optional is enabled 50115191 07 2008 71 HERAcell 150i 240 i 00 xxx 2008 00 00 Temp 3 6 9 B vere atest
21. 50115191 07 2008 77 Operating Instructions HERAcell 150i 240 7 Handling and control Fig 40 To set the cursor to the left to overwrite a value 5 Press the BACKSPACE key lt lt 6 6 To accept and save the input value gt Press the ENTER key 5 gt The system returns to the Settings menu NOTE Changing the user defined code The user defined code can be changed as often as required using the same procedure Activate the recoding function by entering the valid code enter the new code and confirm it Setting date time The input dialog allows date and time to be set to the required time zone 1 Fig 41 To enter the date gt Press the numeric key 2 gt The input numbers are displayed in the input box 1 2 To set the cursor to the left to overwrite a value gt Press the BACKSPACE key lt lt 4 3 To accept and save the input value gt Press the ENTER key 3 4 The system returns to the Date Time menu 5 Enter the time accordingly 6 To accept and save the input value gt Press the ENTER key 3 gt The system returns to the Date Time menu 50115191 07 2008 78 Menu gt Settings gt Date Time gt Date gt Time HERAcell 150 i 240 i 00 xxx 2008 00 00 01010 D Date t0 LS C C C GG C Fig 41 Date time setting Operating Instructions HERAcell 150i 240 7 Handling and control Setting the contras
22. Description Note Device status 1 error status 33 bytes hexadecimal value 2000 of control loops for in format temperature XXXXXXXX XXXX ve CO O rh ref temp UOXXXX XXXX XXXX 23 bytes decimal value in Set actual and reference y 2010 format temperature 2 XXX XX XXX XX T XXXXX 15 byt decimal value i 2020 Set and actual CO content 2 yes format XxXX XX XXX XX 15 byt decimal value i 2030 Set and actual O content 2 decimal value in format XXx XX XXX XX 204a Actual water level 7 bytes decimal value in 10096 or 096 format xxx xx 204b Low humidity indicator 1 2 bytes hexadecimal value in active 0 inactive format xx 1 Example of device status and error status of control loops details see error message table 2 All values have 2 decimal places 131 Operating Instructions HERAcell 150 i 240 i Thermo SCIENTIFIC 15 4 2 Reading internal function parameters Reading internal functions Status of run 1 and remainin f g 25 bytes decimal value in run time hours minutes for 2100 n format disinfection also date and time XX TXXX XX XX XX XX XX XX of last start Status of run 1 current CO 25 bytes decimal value in 2105 offset waiting period format minutes seconds autostart XX XXX XXX XX XX XX XX XX also date and time of last start XX 2132 Read bottle turning device 8 bytes hexadecimal speed all levels 2 value i
23. Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 Error table display eee eeeeeeeaeae eae ee eset tea 4 4 8858 84 Alarim relay Setting 86 ERFa re AREA 85 Low humidity setting cece cece eee 4 eee ae ee eee ee eene 86 Gas tight screen setting 8 5556594996 87 Water level setting 4 4 anaa nnn nnns 67 Alarm relay Setting AE RF REETARE EA 88 Bottle turning device speed 86 1 6 444445 49 45999 54 4 859656 88 Switching the O control on and off 89 Icon description overview 5 5 nennen nennt 90 Gas monitoring 0 8 ennt einen nnne 91 Enabling disabling the keypad 06 55 559 955559559 955555999 94 92 Software versions Ennan Eanna 93 Trend display scaling
24. e g for a value of 60 s default value the events of about 5 days can be stored The data logger stores the following information e important user actions system events and error messages e measured data of the three control loops during incubation operation The data logger can be queried using the following commands Query 2400 00 cc lt CR gt Setting the data logger reading pointer to the oldest entry and output of the first data sets Query 2401 00 cc lt CR gt Output of the following data sets the reading pointer automatically moves gradually from the older entries to the current entries Query 2402 00 cc lt CR gt Repeated output of the most recently read data this command does not move the reading pointer This command can be used to avoid data loss after a communication error has occurred Each query command is responded with up to 7 data sets that follow one another without a separator These data sets consist of 16 bytes and are encrypted into 32 ASCII characters prior to transmission For example the byte 0x23 is converted into the ASCII characters 0x32 2 and 0x33 3 Therefore a response consists of up to 7 X 16 112 bytes i e 224 ASCII characters A data set always delivers the date and the time without seconds the device status and the type of data logger entry byte 0 7 or ASCII character 0 15 Also depending on the entry the current actual values or set values of the control
25. n 5 return 0 50115191 07 2008 146 Operating Instructions HERAcell 150i 240 hermo 15 Annex Data communication 15 8 Program Heracell 150 i amp 240 i User surface for data communication The program provides a user surface only with English menu designation for handling the data communication between a device and a connected PC Sf HERAcell 150 amp 240 HERAcell 150i amp 240i It is strongly recommended that you edt al program before running this instaler Appkcabons that un in the background such as vius seannnig i 822985 might cause the installer to take longer than ev erage to complete Sas This program is used for Reading and archiving error messages error logger The data sets are stored in the meta format CSV reading and archiving event entries data logger The data sets are stored in the meta format CSV Creating a service file servicefile to be sent to the Technical Service of Thermo Fisher Scientific The information of the service file is very useful for systematic troubleshooting The data sets are stored in the proprietary format SRF 50115191 07 2008 147 Operating Instructions HERAcell 150 240i hermo 15 Annex Data communication 15 8 1 Installing the Heracell 150 i amp 240 i Installation routine 1 Starting the installation routine gt On the data CD in the subdirectory PROGRAMS d
26. optional Distributor connection for looping through the CO gas supply for up to three additional devices independent of device type Distributor connection for looping through the O N5 gas supply for up to 3 additional devices independent of device type Diagram Gas connection CO and O4 N with optional gas monitoring 34 Operating Instructions HERAcell 150i 240 hermo 4 Description of the device Fig 8 Rear view HERAcell 240i 50115191 07 2008 35 Operating Instructions HERAcell 150i 240 4 Description of the device Looped through gas supply for HERAcell 240 i 50115191 07 2008 1 2 3 4 5 6 7 Control box with supply interface for combined gas connection optional CO and O5 N5 with gas monitoring optional Distributor connection for looping through the CO gas supply for up to three additional devices independent of device type Distributor connection for looping through the O5 N gas supply for up to 3 additional devices independent of device type Tee joint for fitting the gas pressure hoses together Gas pressure hose for looping through the gas supply Diagram Gas connection CO and O5 N5 with optional gas monitoring HERAcell 240 i with looped through gas supply therefore device must only be equipped with combined gas connection optional CO3 and Oo N gt 36 Oper
27. supply check plug and power supply cable for damage Do not use damaged components when connecting the device to the power supply The device must be connected only to a correctly installed and grounded power supply source Fusing T 10A Circuit breaker G 16 Connection to the power supply system 1 Before connecting the device to the power supply check to see if the voltage of the power supply corresponds with the specifications on the nameplate on the front of the device If the ratings given for voltage V and current A are not correct the device must not be connected to the power supply 2 Connect the IEC connector 2 to the socket 1 of the control box of the device 3 Connect the grounding plug 3 of the power supply cable to a correctly grounded and fused 3 socket oim 4 Make sure the power supply cable is not subjected to tensile or compressive force Fig 27 Power supply connection 50115191 07 2008 59 Operating Instructions HERAcell Data communication via RS 232 Data communication via USB 50115191 07 2008 150 i 240 i Thermo 5 8 Connecting the RS 232 interface The RS 232 data communication interface has been designed for a cable connection with 9 pin connector and a contact assignment of 1 1 Data exchange is accomplished by a predefined structure of command sequences see annex at the end of this section NOTE Data communication diagram The command sequence synt
28. 10 Connecting the alarm contact sessssssssssseseeeeeee nennen 61 6 Operation 63 6 1 Preparing the device sssssssssssssssesseeen enne nennen 63 6 2 Starting operation siisii asied a niaaa daaa Aaa sn nn A 64 7 Handling and control 65 TV dod daaa 65 7 2 Operating panel and operating structure esses 65 7 3 Factory presettings of the controls 485 4 68 7 4 Heat up phase of the control loop 4444454454 ee 4853555566645 68 7 5 Event actions for settings 55 nennen teen 69 7 6 Setting the temperature set value cece eee 5 5 8 70 7 7 Setting the CO set value nennen nennen 71 7 8 Setting the O set value 72 79 11 1 01 1 1011 9 1 1101 tC EC 73 4 9 1 Activating aUto Start iciois inse 5 asa eo d Re 75 7 9 2 Interrupting the auto start routine essssseseeeeeeenenen nnns 76 7 10 Running the contra con 0 11 6 5054985555 4 4 enne 76 7 11 User 6 8 0 5 54
29. 100 deme te 637 p Ite N N N a HERAcell 150 i 100 oO 2 100 us 780 x wo 3 oo E HERAcell 240 i w 100 Fig 1 Device dimensions 934 150 i 240 Thermo 3 Installation of the device 3 4 Transport Operating Instructions HERAcell Fig 2 For transport do not lift the device using the doors or components attached to the device e g control box on rear panel as lift points NOTE Lift points Lift the device only using the lift points 3 5 Stacking Fig 3 HERAcell 150 i 240 i is suited for stacking up to two devices of the same device type For thermal separation an adapter plate 3 can be placed between the two devices Fig 2 Lift points HERAcell 150 i Place the adapter plate with the receptacles 4 at the bottom onto the stacking elements 5 on top of the device Make sure that the adapter plate is seated safely on the stacking elements Place the device to be stacked with the device stands 1 onto the stacking elements 2 at the top of the adapter plate HERAcell 240 i Place the adapter plate with the receptacle recesses 8 at the bottom onto the stacking elements 9 on top of the device Make sure that the adapter plate is seated safely on the recesses Place the device to be stacked with the device stands 6 onto the stacking elements 7 at the top of the adapter plate The devices are secured to one
30. 13 i SIZE DATA2 amp excelstring len excelstring len len num 2 str zahlenstring 14 i SIZE_DATA2 0x100 zahlenstring 15 i SIZE DATA2 amp excelstring len excelstring len enter set values from here len num 2 str SollTemp amp excelstring len excelstring len len num 2 str SollCO2 amp excelstring len excelstring len len num 2 str SollO2 amp excelstring len excelstring len len num 2 str SollrH amp excelstring len excelstring len excelstring len n len 1 WriteFile FileHandle excelstring len break query the remaining events from here case EVENT_FORMAT_DATALOG WriteFile FileHandle excelstring len WriteFile FileHandle Data logger erased n 20 break case EVENT_POWER_ON update of set values SollTemp zahlenstring 8 i SIZE_DATA2 0x100 zahlenstring 9 i SIZE DATA2 SollCO2 zahlenstring 10 i SIZE_DATA2 0x100 zahlenstring 11 i SIZE_DATA2 SollO2 zahlenstring 12 i SIZE_DATA2 0x100 zahlenstring 13 i SIZE_DATA2 SollrH zahlenstring 14 i SIZE_DATA2 0x100 zahlenstring 15 i SIZE_DATA2 WriteFile FileHandle excelstring len WriteFile FileHandle Power on n 10 break case query all events here see chap 15 6 2 pg 140 cancel OxFF indicates the end of the data logger case OxFF WriteFile FileHandle End
31. 2008 00 00 HERAcell 150i 240 i 21 0 49 21 9 HERAcell 150 i 240 i 00 xxx 2008 00 00 contra con instruction 1 Press lt Start gt 2 Open door until buzzer sounds 30s 3 Remove samples from incubator 4 Pump out the water from the reservoir 5 Refill the reservoir with 350 ml water 6 Close door Duration approx 25 hours 00 xxx 2008 00 00 HERAcell 150 i 240 i contra con instruction 1 Press lt Start gt 2 Open door until buzzer sounds 30s 3 Remove samples from incubator 4 Pump out the water from the reservoir 5 Refill the reservoir with 350 ml water 6 Close door Duration approx 25 hours Stop Fig 65 Activating contra con Operating Instructions HERAcell 150i 240 9 Cleaning and disinfection If the STOP key is pressed the routine is interrupted and the contra con Stop dialog box is opened as a security request The routine can now be ultimately cancelled or resumed 2 To cancel contra con gt Press the END key 2 gt The error message is displayed gt If the error message is confirmed the system returns to the main menu 3 To resume contra con gt Press the BACK key 1 gt The system returns to the status display and the decontamination routine is resumed 4 To interrupt contra con from the status display gt Pressthe STOP key 4 gt The contra con Stop dialog box is d
32. Germany Phone Sales 0800 1 536376 Phone Service 0800 1 112110 Fax Sales Service 0800 1 112114 E Mail info labequipment de thermofisher com Enquiries from Europe Middle East and Africa Phone 49 0 6184 90 6940 Fax 49 0 6184 90 7474 E Mail info labequipment de thermofisher com America Postal address USA Thermo Fisher Scientific 275 Aiken Road Asheville NC 28804 USA Enquiries from North America Phone 1 800 879 7767 Fax 1 828 658 0363 E Mail info labequipment thermofisher com Enquiries from Latin America Phone 1 828 658 2711 Fax 1 828 645 9466 E Mail info labequipment thermofisher com Enquiries from Asia Pacific Phone 852 2711 3910 Fax 852 2711 3858 E Mail info labequipment thermofisher com 50115191 07 2008 9 Operating Instructions HERAcell 150 i 240 i I hermo 1 1 Identification of the device and of the documentation Identification of the device Identfication data Device name CO incubator Type designation HERAcell 150 i HERAcell 240 i Serial No 40830469 or higher Allocation of product documentation User information Operating instructions 50115191 Valid 05 2008 Certifications and quality audit Certification CE Certification Test mark GS by VDE Germany CSA UL by VDE Germany 1 2 Instruction of the operating personnel These operating instructions describe the CO incubator HERAcell 150 i 240 i The CO incubator has been manufactured in keeping w
33. HERAcell 150 i 240 i 00 xxx 2008 00 00 Show trend B pik 5 0 02 4 9 Calibration era Set value 2 3 HERAcell 150 i 240 i 00 xxx 2008 00 00 Co2 change set value current 5 0 Q 19 5 4 5 6 Fig 36 Setting the CO set value Operating Instructions HERAcell 150i 240 7 Handling and control 7 8 Setting the O set value This setting is possible only on versions with the optional O5 N control 1 Press the O DISPLAY key 1 gt The O menu is displayed 2 To exit the O5 menu gt Press the END key 2 3 To set the O set value gt Press the SET VALUE key 3 To increase the set value gt Press the key 6 To reduce the set value gt Press the key 4 4 To accept and save the set value gt Press the ENTER key 5 gt The system returns to the main menu The O5 displays shows the actual value currently measured in the work space NOTE Factory settings Depending on the type of the O sensor one of the two O control ranges has been preset at the factory Control range 1 1 to 21 Control range Il 5 to 90 Usage of the process gases For set values below 21 O5 the device must be connected to a nitrogen supply system For set values above 21 O5 the device must be connected to an oxygen supply system Since the O2 concentration of the air is nearly 21 the O2 control and the control loop error monitoring syste
34. Handling and control Error message overview Control Egi Error message Cause Repair Alarm relay Buzzer loop z Gas cylinder changeover Failure gas cylinder 03 switch cannot commuicate Contact Service x x changeover switch with mainboard Repl h No gas Both CO cylinders empty ep ace Gne pr both Oz x x cylinders Gas cylinder A Gas cylinder is empty Replace gas cylinder A Gas cylinder 8 Gas cylinder B is empty Replace gas cylinder B LES eo E O Fill in water or if the auto start Water level in the incubator routine b run dry No water without water the water under level sensor can be switched off 50115191 07 2008 98 Operating Instructions HERAcell 150i 240 8 Shut down 8 1 Shutting the device down 50115191 07 2008 Contamination hazard If the work space surfaces are contaminated germs can be transferred to the environment of the device In case of a shut down the device must be decontaminated Remove the culture containers and all accessories from the work space Pump the water out off the water reservoir see chap 9 Fill in 350 ml water and start the contra con decontamination routine Turn the device off using the power switch when the contra con decontamination routine has been completed Unplug the power connector and protect it against accidental reconnection Close the CO O N gt supply system sh
35. M 24 A Front view HERAcell 150 i 27 5 Rear view HERAcell 150 i 29 6 Looped through gas supply HERAcell9 150 i 31 7 Front view HERAcell 240 i teet 33 8 Rear view HERAcell 240 i tentent 35 9 Looped through gas supply HERAcell 240 i 37 VOX Door cm 40 11 Temperature COs5 and O sensors ssee eee 41 12 Supply interface eT uc et 42 13 Work space components eren 8 4 nennen nnn 44 14 Water 88 0 eene nennen 4 nnne nennen nennen 45 15 Rear panel openings sess enne 8 4 eee 46 16 Bottle turning device sssssssssssssssesesene nnne nnn 47 17 Support rail 518 8 0 0 81 4 445555 teen 4 4944 4 4 49 18 Shelf support installation 55554 eee 443353 4 nnne 50 19 Installing gas 1 1 8 50545545555555555559 5959 5485855555659569498 8 5 50 20 Installing the bottle turning devices 55555 54 548555 4 9994494444 51 21 Bottle turning 5 664 5555 5555 5444453453 nennen nnn nennen 53 22 Center strut installation cece eect 4 443353 ae ee ee ee
36. actions for settings Key response Pressing a key can increase or reduce a value gradually When the key 4 or the key 6 is kept depressed the system switches to quick run after the key has been kept depressed for more than 3 seconds the quick run speed increases NOTE Saving the settings To save changed values press the Enter key Resetting the settings Unless a user action contact with the pressure sensitive areas and keys occurs within 30 seconds the system automatically exits the menu and the most recently confirmed setting defaults 50115191 07 2008 69 Operating Instructions HERAcell 150i 240 hermo 7 Handling and control 7 6 Setting the temperature set value HERAcell 150 i 240 i ET 1 Press the TEMPERATURE DISPLAY key 1 gt The Temperature menu is displayed 2 To exit the Temperature menu gt Press the EnD key 2 3 To set the temperature set value gt Press the SET VALUE key 3 To increase the set value gt Press the key 6 vera Drain To reduce the set value 1 gt Press the key 4 HERAcell 150 i 240 i 00 xxx 2008 00 00 show trend B pik 37 0 a Temp 3 6 1 9 C Calibration e Set value 2 3 4 To accept and save the set value gt Press the ENTER key 5 gt The system returns to the main menu The temperature displays shows the actual value currently measured in the work space HERAcell 150 i
37. and time to the desired time zone ERROR LOGGER for reading the error messages DATA LOGGER for reading the event entries SERVICEFILE for reading error information and for creating a service file PASSWORD to prevent access to the device parameters of the incubator 50115191 07 2008 149 Operating Instructions HERAcell 150i 240 li hermo 15 Annex Data communication Presetting 50115191 07 2008 Function of the user menu PRESETTING The submenu PRESETTING is used to set the transmission speed and to select the serial port i HERAccll 150 240 COM NoCOM PRESETTING TESTCOM DATE amp TME ERROR LOGGER Current presetting 1 Select a transmission speed within the range of 9 600 to 115 200 baud 2 Select the serial port of the PC If the USB driver is installed the virtual COM port assigned to the USB port can be selected see chap 15 1 2 pg 126 3 Accept the settings gt Press the CHANGE button 4 Save the settings in an INI file gt Press the SAVE TO FILE button NOTE Transmission speeds The transmission speed settings in the user menu PRESETTING and of the device must be identical 150 Operating Instructions HERAcell 150i 240 li hermo 15 Annex Data communication TEST COM Test Com The submenu TEST COM is used for testing the communication connection with the settungs defined in the submenu PRESETTING H HERAccll 1
38. another by their own weight Transporting stacked devices The stacking elements are not locking elements Therefore it is not permitted to transport stacked devices Fig 3 Stacking 50115191 07 2008 24 Operating Instructions HERAcell 50115191 07 2008 9 150 240 Mobile racks NOTE Fastening on mobile racks If the devices are installed onto mobile racks make sure that the rollers 10 are secured with a locking brake during the operation of the incubators and that the rollers are oriented toward the front for increased stability Condensate formation during the operation of stacked devices If stacked devices of type HERAcell amp 150 i are operated at an ambient temperature of more than 26 C condensate formation may occur on the upper device while the contra con decontamination routine is run for the lower device To prevent condensate formation an adapter plate should be placed between the two devices for thermal separation 3 6 Retrofitting Modifications Retrofitting options The following components can be retrofitted to the standard version HERAcell amp 150 i 3 segment gas tight screen the gas tight screen is installed in place of the glass door door hinge replacement on outer door and glass door RH LH door opening lockable outer door split inserts infrared IR sensor PCB for USB interface HERAcell amp 240 i 6 segment gas tight screen the gas tight screen
39. be adapted to a lower value by enabling the Low Humidity feature The relative humidity in the work space is lowered from approx 93 to approx 90 The modification requires an extended adaption phase To ensure that it effectively prevents dew formation on culture containers it must be used as a permanent setting CO supply To ensure the growth conditions for the cell and tissue cultures the work space is supplied with CO3 The pH of the bicarbonate buffered culture media largely depends on the CO content of the work space atmosphere The CO content of the work space atmosphere can be controlled within a range of 0 to 20 The supplied CO must have one of the following quality characteristics Purity 99 5 min medical gas quality 03 supply If the CO incubator is to be operated with more than 21 oxygen the work space is supplied with oxygen The O gt content of the work space atmosphere can be controlled within a range of 0 to 21 N supply If the oxygen content during operation is to be lowered to less than 2196 air oxygen content the work space is supplied with nitrogen The Os content of the work space atmosphere can be controlled depending on the configuration of the sensor within a range of 1 to 21 and 5 to 90 39 Operating Instructions HERAcell 150 i 240i li hermo Description of the device 4 7 Door switch A door switch 1 is installed at the upper edge of the work space opening I
40. calibration To determine the exact measured value of the device integral CO sensor a CO comparison measurement has to be performed every three months If a major deviation is found during this check a CO calibration is required During this process the CO control of the device is set to the value measured during the comparison measurement Use a calibrated measuring instrument with an accuracy of 0 3 CO for this test Suitable instrument Portable IR readout instrument The measuring sample is withdrawn through the sealable measurement opening of the glass door The comparison measurement must be performed when the device is completely heated up Comparison measurement procedure 1 Turn the device on using the power switch 2 Set the CO set value and start the device with auto start 3 Insert the measuring instrument probe through the measurement opening 1 into the work space Wait until the CO value displayed by the instrument has stabilized 50115191 07 2008 Fig 70 Preparing CO calibration Operating Instructions HERAcell 150i 240 4 For devices equipped with the optional gas tight screen the measurement opening is located onHERAcell 150 i 1 in the center gas tight screen on HERAcell 240 i 2 in the left center gas tight screen 5 Remove the measuring probe plug the measurement opening and close the doors 6 Calibrate the CO control NOTE IR measur
41. data set always delivers the date the time the faulty control loop the device status and the error message Example of a response HASDOSPSTUSDLORUISISHZDUQUUA ODETUOOISETODODI 80 First data set 1 2300 fb 10501060f372280000002 with 21 bytes Second data set 20b01060f38100001 80 beginning of the second data set after 21 bytes of the first data set and of the separator 1 byte 134 Operating Instructions HERAcell 150 i 240 i Thermo 15 5 1 Error memory data set structure scheme 00 Ob 01 06 Of 37 22 8000 0002 Faulty control loop type 0x00 Error in temperature control loop 00 Date Day 0x0b 11th day of the month Ob Date Month 0x01 January 01 Date Year 0x06 2006 06 Time Hours OxOf 15 hrs Of Time Minutes 0x37 55 minutes 37 Time Seconds 0x22 34 seconds 22 Device status 0x8000 Device error existing 8000 Error message 0x0002 Value above set value upper limit 0002 Therefore the following information is transmitted in this data set Created on 1 January 2006 at 15 55 34 hrs A device error has occurred and the set temperature is too high 15 5 2 Overview of the possible error messages in hex coding Description Type Temperature control loop Hex coded error message 50115191 07 2008 135 Operating Instructions HERAcell 150 i 240 i Thermo 15 5 3 Overview of the possible
42. device optional 14 Gas humidification optional 15 Water level sensor 16 Insert for bottle turning device optional 17 Nameplate 18 Cylinders optional 19 Power switch 20 Support rail 21 Shelf 22 Latch glass door 23 Support hook for shelf 24 Pressure compensation opening with insert 25 Access port with plug 26 Glass door seal replaceable 27 Operating panel touchscreen 28 Door handle 32 Operating Instructions HERAcell 150 i 240 hermo Description of the device Fig 7 Front view HERAcell 240i 50115191 07 2008 33 Operating Instructions HERAcell 150 i 240i 4 Description of the device 50115191 07 2008 Rear view 4 4 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Rear view HERAcell 240 i Pressure compensation opening Access port 42 mm Condensate drain gutter Control box with supply interface for combined gas connection optional CO and O5 N5 without gas monitoring Diagram Gas connection CO and O5 N5 without gas monitoring O5 N gas cylinder CO gas cylinder Secondary CO gas supply B with optional gas monitoring Primary CO gas supply A with optional gas monitoring Secondary O N gas supply B with optional gas monitoring Primary O2 N gas supply A with optional gas monitoring Control box with supply interface for combined gas connection optional CO and O N with gas monitoring
43. error messages in bit coding Bit coded error messages for General device status control loop for temperature and CO temperature and CO 50115191 07 2008 0x0002 Device door open too long 0x0004 Display fails to communicate 0x0008 Mainboad parameter implausible EEPROM faulty 0x0010 Data logger faulty device remains operative Device error has occurred info 18 Temperature controlloop error status 0x0100 Measuring cell parameter implausible EEPROM faulty 0x0020 Error in disinfection contraCon CO control loop error status Br 136 Operating Instructions HERAcell 150 240 i 15 Annex Data communication Bit coded error messages for 2 and water level 50115191 07 2008 Control loop for O and water level 0x0001 0x0002 0x0004 0x0020 0x0040 0x0080 0x0200 0x0400 0x0001 CO control loop error status Sensor breakage Value above set value upper limit Value below set value lower limit Communication error to sensor Gas cylinder changeover switch fails to communicate No gas cylinders A and B empty Gas cylinder A empty Gas cylinder B empty Water level error status No water Operating Instructions HERAcell Data logger data set structure 50115191 07 2008 150 i 240 i Thermo 15 6 Data logger structure The data logger can store up to 10 000 events Depending on the setting for the logging cycle in sections of seconds
44. external alarm system must only be carried out by adequately trained and authorized expert electrical telecommunication personnel Function When system errors and failures occur in the temperature or gas control circuits an alarm message is issued to the connected alarm monitoring system The potential free contact 1 changeover contact has been designed for the following circuits Alarm relay Circuits with system voltage max 250 V Contact Contact Operating state power failure off Operating state power failure o Failure power failure off Failure power failure on X X Contact closed O Contact open NOTE Switching structure The alarm relay switches for all failures reported by the control loops see chap 7 13 pg 95 50115191 07 2008 61 Operating Instructions HERAcell 150i 240 hermo Connection example The connector 5 for the connecting cable is included in the standard equipment The values for the operating voltage of the external circuits and of the fusing of the alarm system are given in the table on the previous page 1 Connect the individual conductors 1 to 4 of the connecting cable as shown in the wiring diagram 2 Connect the connector 5 of the alarm system connecting cable to the interface 6 at the control box at the rear panel of the device Fig 28 Connection example 50115191 07 2008 62 Operating Instructions HER
45. in these locations Use the check boxes below to limit or expand the default search which includes local paths and removable media The best driver found will be installed Search removable media floppy CD ROM Include this lacation in the search C Temp KC_64Bit_Treibe SSD710_USB_x64 Se Browse Don t search will choose the driver to install Choose this option to select the device driver from a list Windows does not guarantee that the driver you choose will be the best match for vour hardware lt Back Nen Cance 3 On the data CD select the DRIVER sub directory Browse For Folder Select the folder that contains drivers for your hardware 8 My Documents a SQ cis 3v Floppy A Festplatte PC15 C amp 4 HERAcell v01 D soB C3 amd64 i386 Programs DE es C3 FR To view any subfolders click a plus sign above 4 The installation routine installs the driver EVAL22 Board USB After the installation has been completed successfully the routine is completed with FINISH The interface transmission speed can be selected within the defined baud rates 9 600 19 200 38 400 57 600 Baud at the touchscreen of the HERAcell 150 i 240 i see chap 7 11 1 pg 77 50115191 07 2008 128 Operating Instructions HERAcell Protocol characteristics 50115191 07 2008 150 i 240 i 15 2 Structure of the command sequences for da
46. inserted into the right roller guide The position and the number of the idle rollers in the frame then depend on the cylinder diameter and on the number of cylinders The figure shows three examples of different idle roller positions in dependence of the cylinder diameter The arrangement of the roller inserts in the work space is designed alphabetically as a b c and d with a being the lowest and d being the uppermost roller insert The connections of the drive rollers are assigned correspondingly to the device control unit The drive roller of the switching insert a must only be connected to the lower receptacle a of the corresponding control unit NOTE Connection assignment If the drive roller is not properly connected to the control unit the parameters cannot be set correctly on the operating panel of the device 50115191 07 2008 47 Ye e e E LOXCXOKO CY oy ro edo TA EN j EA DEAA yY X EO LORA A 0797 Fig 16 Bottle turning device Operating Instructions HERAcell 150i 240 li hermo 4 Description of the device Receptacles To prevent corrosion due to moisture in the work space unassigned receptacles of the bottle turning device must always be plugged with the protective cap The rotating speed of the bottle turning devices is set depending on two parameters Diameter of the cylinders used drive ro
47. rack that supports the rollers may be left in place for the duration of the cycle Procedure of a contra con decontamination routine 1 After cleaning reinstall the shelf system components into the work space 2 Fill the floorpan with 350 ml processed water 3 Turn the device on using the power switch 4 Activate and start the decontamination routine 5 After the decontamination routine has been completed remove the remaining water using a sterile cloth 6 Turn the device off or resume operation Hot surfaces The handle and the screen of the glass door the interior panel of of the outer door as well as the surfaces of the shelf system and ee ofthe work space become extremely hot during the contra con decontamination routine During the routine run or immediately after completion of the run always wear safety gloves when touching these surfaces Damage to the samples During the contra con decontamination routine the work space is heated up to 90 C Make sure that all samples are removed from the work space all accessories are removed from the work space 105 Operating Instructions HERAcell 150i 240 Operating phases of contra con decontamination The remaining run time of the contra con decontamination routine describes the time between the start or the current time status to the end of the drying phase The indicated remaining run times are not measured values but merely used for ori
48. safety devices have been reinstalled and checked for their correct function 10 2 Service intervals During running operation the following service works must be performed 3 month service Run auto start routine and contra con decontamination routine e perform temperature and CO4 O5 comparison measurement Annual service Replace gas inlet filter Perform Technical Service service inspection NOTE Service contract Thermo Scientifc offer a device specific service contact that comprises all test and service works required 50115191 07 2008 110 Operating Instructions HERAcell 150i 240 10 3 Preparing the temperature calibration To determine the exact measured value of the device integral temperature sensor a temperature comparison measurement has to be performed every three months If a major temperature deviation is found during this check a temperature calibration is required During this process the temperature control of the device is set to the value measured during the temperature comparison measurement Use a calibrated measuring instrument with an accuracy of gt 0 1 C for this test To minimize temporary temperature fluctuations during the measurement the measuring instrument is placed into the work space in an isothermal container e g a bowl filled with glycerol The center of the work space is the reference location for the comparison measurement NOTE Isothermal cont
49. stop ring can be positioned accordingly on the roller Set speed of the bottle turning device 7 The rotating speed of the bottle turning devices is set depending on two parameters Diameter of the bottles used drive roller set value Use of diagram 8 The diagram shows the common bottle diameters as different graphs ascending straight Setthe desired speed at the Y axis Read the corresponding set value in 96 from the X axis at the point of intersection of the Y value with the specific graph 50115191 07 2008 51 Operating Instructions HERAcell 150 240i li hermo Example Bottle diameter 56 mm Desired speed 3 5 rpm Determined set value 48 In the dialog box SPEED BOTTLE TURN DEVICE the value 48 is set see chap 7 11 3 pg 85 50115191 07 2008 52 Operating Instructions HERAcell 150 240 i li hermo Adjustment for bottle turning device s Flaschendurchmesser bottle diameter 186mm Flaschendurchmesser bottle diameter 136 mm Flaschendurchmesser bottle diameter 110 mm 3c Flaschendurchmesser bottle diameter 101 mm Flaschendurchmesser bottle diameter 86 mm e Flaschendurchmesser bottle diameter 56 mm E a pen o o a ue o e o 5 G 42 o
50. the device data ready 50115191 07 2008 95 Operating Instructions HERAcell 150 2401 i hermo Error message overview Control loop Error message Cause Repair Alarm relay Device door open Doors open for more than 10 System P Close device doors too long minutes Communication between Contact Service when failure Failure display A display and main board faulty is recuring Failure EEPROM EEPROM on main board is Contact Service main board faulty SCIENTIFIC Failure Communication between communication display and main board faulty mainboard 1 Contact Service when failure is recuring Failure during recording data Reset device to cancel failure logger Device is still When failure is recuring working contact Service System Failure data logger Reset device to cancel failure Failure contra con Failure in contra con routine When failure is recuring contact Service System Power f y Power failure during contra Reset device and start contra down during contra X con routine con again con Failure upon auto Failure during auto start Reset auto start When failure start routine is recuring contact Service Reset device When failure is System Safety Temperature signal VE POM listed several times contact circuit active plausibility doubtful 2 Service Failure Communication between bottle turning bottle turning device and Contact Service x x device main
51. the work space affects the beginning of the temperature control range When additional heating sources are introduced into the work space condensation e g at the glass door may occur Introduced Control range of the temperature energy Example RT 21 C 50115191 07 2008 Fig 15 Rear panel openings 46 Operating Instructions HERAcell 150i 240 4 10 7 Shelf system Fig 16 The support rails 1 of the shelf system are perforated every 42 mm so that the support hooks 8 can be inserted variably for any culture container size required The shelves 2 have an integrated tilt protection and withdrawal stop For details of the shelf system see Section Start up 4 10 8 Bottle turning device for HERAcell 240 i optional Fig 16 HERAcell 240 i may be equipped with up to four separate bottle turning devices Each unit consists of a roller insert 6 with a drive roller 4 and four idle rollers 7 that are also rotated due to the transmission function of the inserted cylinders 5 Each unit is controlled separately as the drive roller is connected to the device control unit by a connector 3 at the right side of the device This modular layout also allows the operation of only one bottle turning device in combination with several shelves The arrangement of the idle rollers in the roller insert frame depends on the configuration of the cylinders used The drive roller is always
52. view HERAcell 150 i Access port 42 mm Pressure compensation opening Control box with supply interface for combined gas connection optional CO and O2 N5 without gas monitoring Condensate drain gutter Diagram Gas connection CO and O5 N5 without gas monitoring O5 N gas cylinder CO gas cylinder Secondary CO gas supply B with optional gas monitoring Primary CO gas supply A with optional gas monitoring Secondary O N gt gas supply B with optional gas monitoring Primary O2 N gas supply A with optional gas monitoring Control box with supply interface for combined gas connection optional CO and O N with gas monitoring optional Distributor connection for looping through the CO gas supply for up to three additional devices independent of device type Distributor connection for looping through the O N gas supply for up to 3 additional devices independent of device type Diagram Gas connection CO and O5 N with optional gas monitoring 28 Operating Instructions HERAcell 150 240 i li hermo x 8 Sl Fig 5 Rear view HERAcell 150 i 29 50115191 07 2008 Operating Instructions HERAcell 150i 240 4 Description of the device Looped through gas supply for HERAcell 150 i 50115191 07 2008 1 2 3 4 5 6 7 Control box with supply interface for combined gas co
53. while 1 set data logger to beginning and read if GetTele send_telegramm 2400 else read additional data sets send_telegramm 2401 len get telegramm buffer no telegram received if len GetError requery send_telegramm 2402 len get telegramm buffer again no telegram received if len return 1 increase telegram counter GetTele 144 Operating Instructions HERAcell 150i 240 li hermo SCIENTIFIC 15 Annex Data communication 50115191 07 2008 length of sent payload len ahex buffer 7 0x10 ahex buffer 8 2 converting ASCII string into usable numeric string for 12 0 i len i zahlenstring i ahex buffer 10 2 i 0 10 ahex buffer 11 2 i calculation of the sent data packages data len SIZE DATA2 evaluation of all data packages for i 0 i lt data i 9 len 0 write time and date into file len time 2 str zahlenstring 1 i SIZE_DATA2 amp excelstring len excelstring len len time 2 str zahlenstring 2 i SIZE_DATA2 amp excelstring len excelstring l len len time 2 str zahlenstring 3 i SIZE_DATA2 amp excelstring len excelstring len len time 2 str zahlenstring 4 1i SIZE_DATA2 amp excelstring len excelstring len len time 2 str zahlenstring 5 i SIZE_DATA2 amp excelstring len excelstri
54. with DIN 58 956 Laboratories in the central area of clinics and hospitals The gases required for the incubator CO and or O2 N are supplied to the device from a separate gas supply system either from gas cylinders or from a central pressurized gas container The layout of the gas supply system must ensure that the operating pressure of the gas supply lines can be set to a range between 0 8 bar min to 1 bar max and that the pressure cannot be changed If the device is equipped with the optional gas monitoring system up to four devices can be interconnected in line independent of the of the gas supply system capacity The CO incubator is suitable for continuous operation 1 6 2 Incorrect use Do not use cell or tissue cultures in the device that are not in accordance with the regulations of safety levels L1 L2 and L3 Do not use tissues substances or liquids that are easily ignitible or explosive release vapors that form combustible or explosive mixtures when exposed to air release poisons 1 7 Standards and directives The device complies with the following standards and guidelines DIN EN 61010 1 2002 DIN EN 61010 2 010 2004 Low Voltage Guideline 2006 95 EG EMC Guideline 2004 108 EG UVV BGV D4 Revised Version UVV VBG 20 continues to be valid for old stock The following safety regulations must be observed if the device is operated within the territory of the Federal Republic of Germ
55. with the hygiene regulations set forth by the operator to protect the cultures The Principles of good microbiological proceedings at the annex of these instructions can be used as safety information for personnel working with the device Water supply A sufficient quantity of treated water of the following quality must always be available for the running operation distilled fully demineralized and autoclaved sterile When the filling level drops below a minimum value it must be possible to top up water during the work process Capacities HERAcell 150 i 3 0 e HERAcell 240 i 4 5 63 Operating Instructions HERAcell Starting with auto start Loading the work space 50115191 07 2008 150 i 240 i 6 2 Starting operation 1 w Fill the water tray with a sufficient quantity of processed water Do not exceed the upper level mark Make sure that the CO2 O5 N supply system valves are open Turn the device on using the power switch Set the set values for temperature and CO O content on the operating panel Start contra con Starting the device 6 7 10 11 Start the device with the auto start routine see chap 7 9 1 pg 75 The progress indicator auto start appears on the display and the automatic start routine is run The temperature control adjusts the temperature to the selected set value humidity rises When temperature and relative humidit
56. works Duration of the routine Running the routine usually takes 5 to 7 hours At low room temperatures and when the device is cold it may take up to 10 hours until the auto start routine has been completed If the glass door is opened or if the power supply of the device is interrupted while the routine is running the routine is interrupted and rerun after the glass door has been closed or the power supply has been reestablished Start conditions for the auto start routine Prior to running the auto start routine set the CO and Os set values to the desired values and make sure that the atmosphere in the work space only consists of ambient air The floorpan of the device must be filled with a sufficient quantity of water Conditions that prevent the start of the auto start routine The auto start routine cannot be started if one of the following failure conditions exists Temperature control loop Sensor breakage actual value above set value excessive deviation actual value below set value excessive deviation 50115191 07 2008 73 Operating Instructions HERAcell 50115191 07 2008 150 i 240 i set value not plausible calibration values too high or too low sensor communication failure sensor parameter not plausible no communication with LM 75 Control loop for CO gas supply No communication with sensor In this case the auto start key is dimmed and its function is n
57. 0 If the device has been configured at the factory for the bottle turning device function the main menu displays the bottle turning device icon The speeds of the drive rollers can be set separately for each level within a value range of 0 to 100 see chap 4 10 8 pg 47 The drive roller of a bottle turning insert is activated as soon as a value 0 for the speed has been entered and saved 1 To increase the value gt Press the key 4 2 To reduce the value gt Press the key 2 3 To switch the drive roller of one level off gt Set the value to 0 4 The value change appears in the display 1 5 To accept and save the change gt Press the ENTER key 3 gt Thesystem returns to the Bottle Turning Device menu 50115191 07 2008 Menu gt Options gt Buzzer HERAcell 150 i 240 i 00 xxx 2008 00 00 Audible alarm current on Fig 55 Alarm relay setting Menu gt Options gt Bottle turn device gt Bottle turn device d gt Bottle turn device c gt Bottle turn device b gt Bottle turn device a 1 HERAcell 150 i 240 i Fig 56 Bottle turning device speed setting Operating Instructions HERAcell 150 i 240i 7 Handling and control NOTE Quick access to bottle turning device levels To directly access the Bottle turning device submenu touch the Bottle turning device icon in the main menu Switching the O control on and off Dependin
58. 00 00 Show trend Showing the trend 1 i Set value Setting value co 4 9 o 21 2 A B Starting j Date Setting date Keypad lock code Changing code Ing a Date Time Time Setting time Contrast Adjusting contrast Key tones Adjusting key tones Interface 85232 Setting baud rate Interface Interface USB Setting baud rate Language Selecting language 1919 contra con Setting day auto start Setting day Service interval Setting day Event display Indicating event records Recording interval Adjusting recording interval Error table Indicating error informations Alarm relay Alarm relay power failure ON OFF Low humidity Low humidity ON OFF Gas tight screen optional Gas tight screen ON OFF Water level sensor Water level sensor ON OFF Buzzer Buzzer ON OFF Bottle turn device optional O2 optional 02 ON OFF Bottle turn device d Setting rotating speed level d Bottle turn device c Setting rotating speed level c Bottle turn device b Setting rotating speed level b Bottle turn device a Setting rotating speed level a Description of icons L Activating keypad lock Indicating software versions Fig 33 Menu structure overview 67 Operating Instructions HERAcell 150 240i 7 Handling and control 7 3 Factory presettings of the controls Upon delivery of the d
59. 4 bytes empty Status gas monitoring 2 bytes 4 Bytes empty Status gas monitoring 2 bytes 4 Bytes empty Current actual values for temperature CO 22 and rh Current actual values for temperature CO O and rh Drive roller speed status 2 bytes each Drive roller speed status 2 bytes each Current actual values for temperature CO 232 and rh No information not even on date time and status Operating Instructions HERAcell 150 240i li hermo 15 Annex Data communication 15 7 Examples of data logger codes Data logger entry Anentry inthe data logger is 16 bytes large and has the following structure 1 byte indicates the event e g door open 0x31 measured value entry 2 byte day of entry 3 byte month 4 byte year 5 byte hour 6 byte minute 7 and 8 byte device status 9 to 16 byte various data on the event 15 7 1 Functions for data logger queries Functions The following code example for reading the data logger uses six functions ahex converts the received ASCII character into a hexadecimal number send_telegramm sends a query to the data logger get_telegramm receives a response data the data logger time 2 str uses a hexadecimal value to create ASCII characters in time format num 2 string uses hexadecimal values to create ASCII characters to be entered into a file read datalogger edits the received data and writes them into a
60. 50 240 COM NoCOM eg 2010 2010 attachment e g 037 00 sing sent 7 2010360000090900009 received 2010 037 00 036 75 036 78 1 Example of a query for the currently measurable temperature values of the incubator gt Query default not changeable gt Address 2010 temperature value address Set value actual value reference value 2 Send the query to the incubator 3 Press the SEND button gt If the incubator returns a response string the communication connection to the incubator has been established gt If a connection cannot be established an error dialog is displayed 4 Exit the error dialog gt Press the OK button 50115191 07 2008 151 Operating Instructions HERAcell 150 i 240i li hermo 15 Annex Data communication DATE amp TIME Date amp Time The submenu DATE amp TIME is used for adjusting date and time to the desired time zone I3 HERAccll 150 240 COM NoCOM GENERAL DATEATME e ERROR LOGGER iii pitii DATA LOGGER iii uss SERVICEFILE ss uss PASSWORD 1 Data in the two text boxes must be entered in the format DD MM YY day month year 2 Accept the input data gt Press the SET button ERROR LOGGER Error Logger The submenu ERROR LOGGER is used for reading the error messages into the text box of the user surface HERAcell 150 240 COM NoCOM EST MAIN GENERAL PRESEN
61. Acell 50115191 07 2008 Device check Decontamination 150 i 240 i Thermo 6 1 Preparing the device The device must only be released for operation after all major measures for the start up have been taken see Section 5 page 45 Device check Prior to starting operation the following device components must be checked for their correct function e Gas hoses must be seated tight on the connecting filter and must be secured using a hose clamp The access port must be capped The pressure compensation opening must be permeable its insert must be installed in the work space The glass door gas tight screen seal must not be damaged The glass door measurement opening must be capped The shelf system components must be installed safely The gas humidification optional must be connected to the device integral gas supply and aligned parallel to the rear panel The drive rollers ofthe bottle turning device optional must be properly connected to the corresponding control unit The idle rollers of the bottle turning device optional must be positioned in accordance with the cylinder diameters Decontaminating the device work space Run the contra con decontamination routine see chap 9 4 pg 104 or decontaminate the work space according to the hygiene regulations set forth by the operator NOTE Hygiene regulations Prior to any operation the user must clean and disinfect the work space in accordance
62. Decontamination Condensation Cooling down Postheating Temp 90 C Fig 64 Decontamination routine phases Operating Instructions HERAcell 150i 240 9 Cleaning and disinfection 9 4 1 Activating the contra con routine Fig 65 The contra con decontamination routine is used to decontaminate the complete work space 1 Press the CONTRA CON key 1 gt The contra con instruction menu 2 is displayed 2 To exit the contra con instruction menu and contra con gt Press the END key 4 gt The system returns to the main menu 3 To activate contra con gt Press the START key 3 gt The contra con instruction menu 2 is displayed 4 To air the work space open both device doors when the audible alarm sounds after 30 seconds 5 Remove all samples from the work space 6 Pump the water out of the floorpan and wipe off water residues 7 Fill the floorpan of the work space with 350 ml processed water 8 Close both device doors gt The run of the contra con decontamination routine starts Fig 66 While the contra con decontamination routine is running the display shows the current status 5 and outputs the following information Temperature start time phase remaining run time 9 4 2 Interrupting contra con Fig 65 The contra con decontamination routine can be interrupted at any time 1 To interrupt contra con gt Press the STOP key 6 50115191 07 2008 107 00 xxx
63. Interval menu On the due date the display shows a reminder message 5 for the activated reminder interval contra con Please run contra con auto start Please run auto start Is displayed after the contra con decontamination routine has been run successfully 21 0 Service interval Request service The service 4 9 o 21 9 4 message can be confirmed In this case the Request Service icon is displayed After the routines have been run successfully the reminder messages are hidden Factory settings Please run contra con contra con decontamination routine 90 days auto start routine Off 5 Service interval 365 days Fig 47 Reminder interval setting 50115191 07 2008 82 Operating Instructions HERAcell 150i 240i 7 Handling and control 7 11 2 Event logging The input dialogs of the category EVENT LOGGING comprise all settings for logging and outputting events during the operation of the device e Event display time interval of the logging cycle error table Event display The event display 1 uses short single line entries specifying date and time to report the events that were logged during the operation of the device The entries are listed in chronological order with the most recent event at the top position The list can be displayed but not edited If the event display consists of several pages the user can browse through the list The status indicator 2 indicates wh
64. Operating Instructions CO Incubator HERAcell 150 i 240 i with Decontamination Routine HERACELL 240i Oeste HERACELL 150i CO newton SCIENTIFIC Valid 50115191 07 2008 Copyright These operating instructions are protected by copyright Rights resulting thereof particularly reprint photomechanical or digital postprocessing or reproduction even in part are only allowed with the written consent of the Thermo Electron LED GmbH This regulation does not apply to reproductions for in plant use The contents of the operating instructions are subject to change without further notice Concerning translations into foreign languages the German version of these operating instructions is binding Trademarks HERAcell is a registered trademark of Thermo Scientific Thermo Scientific is a brand of Thermo Fisher Scientific Inc All trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers Thermo Electron LED GmbH Robert Bosch Strafe 1 D 63505 Langenselbold Germany Thermo Electron LED GmbH is a subsidiary company of Thermo Fisher Scientific Inc 81 Wyman Street Waltham MA 02454 USA Operating Instructions HERAcell 150 i 240 i 50115191 07 2008 1 General notes 1 1 Identification of the device and of the documentation ssssss 1 2 Instruction of the operating 6 80 61
65. RAcell 150 i 240 i 00 xxx 2008 00 00 Language setting D current German English 2 3 4 1 To browse upward in the selection gt Press the key 4 2 To browse downward in the selection gt Press the key 2 3 The new language appears in the display 1 4 To accept and save the selection gt Press the ENTER key 3 gt The system returns to the Settings menu Fig 46 Language setting 50115191 07 2008 81 Operating Instructions HERAcell 150i 240 li hermo 7 Handling and control Setting the reminder intervals The reminder intervals are integral components of the a ae alarm and monitoring system of the device control gt Reminder intervals For the two essential functions contra con and auto gt contra con start as well as for routine service work the user can gt auto start set dates that trigger an alarm whenever they occur gt Service interval The counting begins at 00 00 hrs of the day on which the previously set reminder interval has elapsed 1 To increase the number of days HERAcell 150 i 240 i 00 xxx 2008 00 00 gt Press the key 4 2 To reduce the number of days gt Press the key 2 3 The value change appears in the display 1 4 To deactivate the reminder interval gt Set the value to OFF gt Press the key 2 5 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Reminder
66. TING TESTCOM DATES TIME ERROR LOGGER DATALOGGER SERVCEFLLE PASSWORD save to The data sets can be stored in the metaformat CSV 50115191 07 2008 152 Operating Instructions HERAcell 150 240i hermo 15 Annex Data communication e Saving data sets as files gt Press the SAVE TO FILE button DATA LOGGER Data Logger The submenu DATA LOGGER is used for reading the event entries into the text box of the user surface I3 HERAcell 150 240 COM NoCOM Pim E MAIN GENERAL PRESETTING TESTCOM DATE amp TME ERROR LOGGER DATALOGGER SERVICEFILE PASSWORD The data sets can be stored in the metaformat CSV 1 Read the data sets gt Press the READ button 2 The progress of the data transmission is indicated in the three text boxes gt AMOUNT DATA Total number of transmitted data sets AMOUNT TELEGRAMS Therein the number of transmitted event entries AMOUNT ERRORS Therein the number of transmitted error messages NOTE Duration of the data transmission As the data logger can contain up to 10 000 data sets the data transmission to a PC may take some time 50115191 07 2008 153 Operating Instructions HERAcell 150i 240 li hermo 15 Annex Data communication SERVICEFILE Servicefile The submenu SERVICEFILE is used for reading error informations and for creating a service file from it saved with the p
67. TION key 2 4 Enter the measured value targeting value The targeting value can be increased or reduced in increments if you keep the key 4 or the key 6 depressed the function switches to a rapid increase reduction after approx 3 seconds another increase reduction occurs To increase the targeting value gt Press the key 6 To reduce the set value gt Press the key 4 5 To accept and store the targeting value gt Press the ENTER key 5 gt The system returns to the main menu The temperature display shows the current actual value measured in the work space NOTE Excessive work space temperature Excessive work space temperature after the calibration can be reduced by leaving the doors open for approx 30 seconds 50115191 07 2008 HERAcell 150i 240 i 00 xxx 2008 00 00 d M 36 9 i B ia 02 4 9 SL 02 19 9 Ce 3 aaa HERAcell 150 i 240 i 00 xxx 2008 00 00 show trend B Toon 37 0 Temp 37 1 0 C 6 6 ex 2 3 HERAcell 150 i 204 i 00 xxx 2008 00 00 Temp calibration i current 37 0 36 4 4 5 6 Fig 69 Temperature calibration procedure 112 Operating Instructions HERAcell 150i 240 Value reset If the value isn t changed within the next 30 seconds the system automatically exits the menu and the most recently confirmed value is preserved 10 5 Preparing the CO
68. ainer Do not use a container filled with water as isothermal container as the evaporation of water will result in a lower temperature reading Excessive work space temperature Excessive work space temperature after the calibration can be reduced by leaving the doors open for approx 30 seconds Comparison measurement procedure 1 Turn the device on using the power switch 2 Set the temperature set value and allow the device to be heated This may take up to several hours 3 Place the measuring instrument 3 onto the center area of the work space Alternatively a temperature sensor may be positioned in this location Route the connecting cable either through the measurement opening 2 in the glass door or through the access port 1 in the rear panel of the device 50115191 07 2008 111 Fig 68 Preparing the temperature calibration Operating Instructions HERAcell 150i 240 10 Maintenance 4 Close the doors 5 Wait until the temperature value displayed on the measuring instrument has stabilized 6 Calibrate the temperature control as described in see chap 10 4 pg 112 10 4 Temperature calibration procedure Measurement example Temperature set value 37 C Reference temperature 36 4 C 1 Press TEMPERATURE DISPLAY 1 gt The temperature menu is displayed 2 To exit the temperature menu gt Press the END key 2 3 To enter the Calibration submenu gt Press the CALIBRA
69. al The stepping rate of both interfaces can be changed within the defined baud rates 9 600 19 200 38 400 57 600 baud The standard stepping rate of both interfaces is 57 600 baud To increase the value gt Press the key 4 To reduce the value gt Press the key 2 The value change appears in the display 1 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Interface menu To activate the new settings gt Browse back to the main menu gt Wait for approx 10 seconds and perform a reset by switching the device off and on using the power switch 50115191 07 2008 80 Menu gt Settings gt Interface gt Interface RS 232 HERAcell 150 i 240 i 00 xxx 2008 00 00 Baud rate RS232 D current 9600 Q 57600 Baud 2 3 4 Fig 44 RS 232 interface baud rate setting Menu gt Settings gt Interface gt Interface USB HERAcell 150 i 240 i 00 xxx 2008 00 00 flO Baud rate USB current 57600 38400 Baud 2 3 4 Fig 45 USB interface baud rate setting Operating Instructions HERAcell 150i 240 li hermo 7 Handling and control Setting the user display screen language Men The input dialog allows the language of the display gt Settings screen to be set Five languages are available gt Language e German English Spanish French Italian HE
70. aning as the antimicrobial effect of the copper material is based on it The components of the shelf system can be removed easily so that only the easily treatable surface reduced interior container 1 remains in the work space for cleaning and manual disinfection works 4 10 2 Optional split gas tight screens Equipment versions HERAcell 150 i with 3 segment gas tight screen 2 HERAcell 240 i with 6 segment gas tight screen 3 For devices that are equipped with the optional gas tight screen the contamination hazard is considerably lower and the recovery times of the incubation parameters are shorter for work space temperature e CO concentration e O N concentration relative humidity Advantage of the gas tight screen The cross sections 50115191 07 2008 Fig 13 Work space components Operating Instructions HERAcell 150i 240 of the openings are smaller when samples are accessed 4 10 3 Water reservoir The water reservoir 1 is integral to the interior container floor and inclines toward the rear The water level is monitored by a water level sensor 2 that issues an alarm message at the display and an audible signal when the water falls below the minimal level The embossings 3 in the water tray are used as indicators for the maximal level 4 10 4 Gas humidification optional only for O control A hose 5 connects the gas humidification 6 to the device integra
71. any 14 Operating Instructions HERAcell 150 i 240 li hermo 1 General notes ZH 1 10 ZH 1 119 ZH 1 342 ZH 1 343 ZH 1 598 TRG 280 EC Official Gazette L 374 Safety data sheets of the gas suppliers with regard to the particular characteristics of CO O5 and No Principles of good microbiological proceedings notice of the trade association of the German chemical industry For other countries the applicable national regulations are binding 50115191 07 2008 15 Operating Instructions HERAcell 150 i 240 i li hermo 1 General notes 50115191 07 2008 Carbon dioxide 1 8 Safety notes on gases NOTE RH Installation work Any work to supply lines and pressurized gas containers cylinders or containers used for storing CO or 02 15 must only be carried out by expert personnel using the appropriate tools Instruction of the personnel Personnel operating devices with CO supply must be instructed about the particularities in the handling of CO before starting their work Correct operation of pressurized gas containers and gas supply systems e obligation to report damages and shortcomings in CO supply lines measures to be taken in case of accidents or failures These instructions must be repeated at appropriate intervals and must comprise the particular operating instructions of the gas supplier 1 8 1 Safety notes on carbon dioxide CO Since CO is r
72. ated as a harmful gas certain safety instructions must be observed when the CO incubator is started up and when the device is operated Suffocation hazard Large amounts of CO released into the room atmosphere may cause suffocation C2 If CO is released initiate safety measures immediately Leave the room immediately and do not allow others to enter the room Inform security service or fire department 16 Operating Instructions HERAcell 150 i 240 i 1 General notes 1 8 2 Safety notes on oxygen O3 032 is a gas that promotes combustion and may explode in combination with grease containing materials 50115191 07 2008 Oxygen Nitrogen Oxygen explosion O may explode in combination with oils greases and lubricants If highly compressed oxygen comes in contact with grease or oil containing substances the mixture may explode Forcleaning these device components use only oil and grease free lubricants Keep all connections and components of the oxygen system free from substances that contain oil grease or lubricant Fire hazard Released oxygen promotes combustion Do not use open flames in the vicinity of oxygen operated systems Do not smoke in the vicinity of oxygen systems Donot expose the components of an oxygen system to excessive heat 1 8 3 Safety notes on nitrogen N3 Nitrogen mixes easily with air High concentrations of nitrogen reduce the oxygen content in the ai
73. ating Instructions HERAcell 150i 240 hermo 4 Description of the device HERAcell 2 4 mpo amp ri TTL ri h pil nents Ln ny y lt 1 HA x Fig 9 Looped through gas supply HERAcell 240 i 50115191 07 2008 37 Operating Instructions HERAcell Temperature conditions 50115191 07 2008 Water quality 150 i 240 i Thermo 4 5 Safety devices The device is equipped with the following safety devices A door switch interrupts the CO2 O5 N supply and the work space heating when the glass door is opened The optional gas detector switches the gas supply over to a full gas cylinder e An independent thermal protection protects the samples from harmful overheating in case of failures A pressure compensation opening ensures pressure compensation in the device work space The alarm relay circuit uses audible and visual alarms to indicate errors during operation 4 6 Work space atmosphere In the work space of the incubator the particular physiological ambient conditions for the preparation and cultivation of cell and tissue cultures are simulated The work space atmosphere is determined by the following factors Temperature Relative humidity e CO concentration e O concentration optional Temperature To ensure undisturbed operation the temperature in the op
74. ature deviation spatial DIN 12880 Part 2 at 37 C 1 Duration of the auto start routine to 37 C ambient temperature 20 C Heat transfer to environment at 37 C during contra con decontamination Humidity demineralized distilled or autoclaved or completely Water qualit a n a y deionized distilled or autoclaved Liquid quantity Incubation operation contra con disinfection operation 350 Constant humidity at 37 C high humidity mode rH approx 93 Constant humidity at 37 C low humidity mode rH approx 90 Sound pressure level DIN 45 635 Part 1 dB A 1 determined on the basis of DIN 12880 for devices with standard equipment See detailed information in calbration instructions max 3 0 min 1 2 ml 50115191 07 2008 120 Operating Instructions HERAcell HERAcell 150 i eins CO gas supply system 150 i 240 i Thermo Gas purity min 99 5 or medical quality Prepressure min 0 8 max 1 Measuring and control range 0 20 Control deviation temporal 0 1 CO measuring cell Accuracy absolute x 0 3 O5 gas supply system Gas purity min 99 5 or medical quality Prepressure min 0 8 max 1 Measuring and control range 1 21 or 5 90 Control deviation temporal 0 2 05 measuring cell Accuracy absolute O i Taar I oe 2 0 Option 5 90 O3 Electrical system 1 N PE 230 V AC 10 Rated voltage 1 N PE 120 V AC
75. ax as well as the RS 232 interface data communication diagram are described in the annex at the end of this section Connecting the devices 1 Turn the PC off 2 Connect the serial interface cable connector not included in the standard equipment to the socket at the device supply interface at the rear of the device 3 Connect the second connector to an unassigned serial port COM 1 COM 2 etc at the PC 4 Turn the PC on 5 9 Connecting the USB interface optional As an alternative to data communication via RS 232 interface the devices can be equipped with the optional USB interface The USB interface complies with Standard USB 1 1 and is compatible with Standard USB 2 0 full speed The interface stepping rate can be changed within the defined baud rates 9 600 19 200 38 400 57 600 baud Data exchange is accomplished via a defined command sequence structure frames The command sequences correspond with the RS 232 interface installation diagram see annex at the end of this section Using the USB interface for data communication between a PC and the device a driver must be installed first see annex data communication 60 Operating Instructions HERAcell 150 2401 I hermo 5 10 Connecting the alarm contact NOTE Skilled work Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly The connection of the device to an
76. board faulty Tempe Detected value is out of p Sensor breakage n Contact Service x x rature limit Act val gt nom val 1 C Do not exceed ambient Actual value above x x 3 temperature limit Act val nom val 1 C Actual value below 4 Contact Service Calibration values Temperature max i Contact Service x x too high too low adjustment value exceeded Actual value MR Temperature signal plausibility iausibilitv doubtful Contact Service x x doubtful P d Failure Measuring cell cannot i Contact Service x x communication commuicate with mainboard 1 Error message will be displayed but not entered in error table 2 Error message will be displayed but not entered in error table 3 If failure is detected a special sample protection controlling will be enabled To indicate the start of this routine the icon overtemperature is displayed The routine will will be disabled after a power reset X X Xx X 4 After changing the set value error time will be set on 159 min after door opening on 45 min O on 159 min 50115191 07 2008 96 Operating Instructions HERAcell 150 2401 i hermo SCIENTIFIC Error message overview Control loop LM75 sensor cannot not Tempe p Failure LM75 communicate with Contact Service x x ratur mainboard sensor Detected value is out of Reset auto start When failure CO Sensor breakage m A i x limit is recuring contact Service
77. centration 4 0 CO 21 2 O3 Example of a code For an example of a code please refer to the end of this chapter 139 Operating Instructions HERAcell 150 2401 I hermo 15 6 2 Overview of possible event entries in bit coding Bit coded event entries Overview of event entries Part 1 Gode Even Specialinformation byte 8 15 Set values from all control NE Current values for temperature loops periodically in minute cycles COs 35 and rh 0x02 Set value change at beginning Set value for temperature CO of new section 03 and rh Set value for temperature CO 0x10 Change temperature set value 05 and rh Set value for temperature CO 0x11 Change CO set value O and rh Set value for temperature CO 0x12 Change O set value O and rh 0x20 Newt i Status error registration for i Ld temperature CO O and rh 0x21 New CO Status error registration for d temperature 0 and rh 0x22 New Status error registration for pos eon temperature CO O and rh 0 2 N i Status error registration for CW SISO m error temperature COs O and rh Set value for temperature CO 0x30 Power reset 05 and rh 0x31 D Current actual values for e 9 open temperature CO 32 and rh 0x32 D nsn Current actual values for 1 DLL temperature CO O and rh Customer specific Calibration level 2 bytes old 0x40 temperatire calibration temperature new tem perature P 2 bytes each
78. cessing infectious substances Therefore the device and device components may have been contaminated Prior to disposal all device components must be decontaminated Decontamination measures Clean the device components thoroughly and either disinfect or decontaminate them depending on application Attach a declaration of non objection with details of performed decontamination measures to the items that are to be disposed of Controlled disposal All device components can be disposed of after they have been decontaminated properly Recycling Service NOTE Recycling Service Thermo Fisher Scientific offer a recycling service for discarded components at the owner s expense 50115191 07 2008 118 Operating Instructions HERAcell 150 2401 i hermo Overview of the materials used plastics equipped on epoxy resin bound boards 50115191 07 2008 119 Operating Instructions HERAcell HERAcell 150 i SO S Gmmbevoume 2 X18 kg 150 i 240 i Thermo Maximal surface load 10 insertion shelf Maximal device overall load Weight without accessories Thermal Class 3 1 overtemperature controller TWW with overtemperature detection function Ambient temperature range 18 33 Temperature control range RT 3 55 Thermal safety devices according to DIN 12880 2007 05 Temper
79. con 44 enne 107 9 4 2 Interrupting contra COn nennen nnnm nennen nnns 107 9 4 3 contra con interruption due to error 4444455554 4 55959 998888946 108 9 4 4 Completing contra con 8 4 458 109 10 Maintenance 110 10 1 Inspections and 6 55 5 5 4453 94 110 10 2 Service intervals deett dint tee 8 e ul esi dedi 110 10 3 Preparing the temperature calibration esses 111 10 4 Temperature calibration procedure ssessssseeseeeneeene 112 10 5 Preparing the CO calibration eesseeennm emen 113 10 6 CO calibration procedure eeeseeeseeeseeeeeneennnnen nennen nnne nnn 115 10 7 Replacing the gas inlet filter essere 116 10 8 Replacing the device fuses 8 4 4 116 10 9 Replacing the door seal aaa 4 nennen 117 11 Disposal 118 12 Technical data 120 13 Device log 124 14 Certificate of d
80. d 2 2 Clamp the lockspring 3 behind the upper embossing 1 3 To remove the support rails pull the lockspring tab down out of the embossing and remove the rail 50115191 07 2008 49 Fig 17 Support rail installation removal Operating Instructions HERAcell 150i 240 5 2 2 Installing the shelf supports 1 Fig 18 Insert the shelf supports 3 into the perforations 1 of the support rail with the bar facing down 2 Make sure that the two vertical elements 2 of the shelf support are flush with the support rail Installing the shelves 1 Push the shelf 4 onto the shelf supports with the tilt protection 5 facing the rear panel of the device The tilt protection 5 is also used as a guide for the shelf 2 Slightly raise the shelf so that the withdrawal stop 6 can be routed over the shelf supports 3 Make sure that the shelf support is positioned in the two tilt protections in a way that allows it to move freely 5 2 3 Levelling the device 1 Position a bubble level onto the center shelf or onto the roller holder 2 Rotate the adjustable device stands using the supplied 24 mm wrench until the shelf is positioned horizontally in all directions Perform the adjustment of the device stands from left to right and from rear to front 5 3 Installing the gas humidification optional only for O N control Fig 19 The gas humidification 1 is installed to the water tray parall
81. d open the dialog box 1 7 11 1 Settings HERAcell 150 i 240 i 00 xxx 2008 00 00 The input dialogs of the SETTINGS category comprise all settings for the individual configuration of the user display screen and the user interface Keypad lock coding Date time setting Contrast setting Key tone setting Interface configuration User display screen language setting Reminder interval setting Changing the keypad lock code The keypad lock prevents the unauthorized changing of the operational settings Only those keys are locked 2 3 4 where values can be entered The keypad is locked by entering four numbers Upon delivery the default code is 0000 This default can be changed into a user defined code that is then enabled using the KEYPAD LOCK dialog box see chap 7 11 5 pg 92 HERAcell 150 i 240 i Code new EC L C q CU CO C C6 CO 4 To confirm the input 5 6 gt Press the ENTER key 3 gt The system changes to the CODE NEW menu 1 To enter the default 0000 gt Press the corresponding numeric keys gt The number combination is displayed hidden in the input box 1 2 To delete the input numbers gt Press the DELETE key 2 3 To exit the menu gt Press the BACK key 4 Fig 40 Changing the code Entering the new 4 digit code gt Press the corresponding numeric keys gt The number combination is displayed in the input box 1
82. device Low water Overtemperature level Gas cylinder Low humidity empty Keypad lock Request service HERAcell 150 i 240 i 00 xxx 2008 00 00 21 0 o 21 9 49 co2 amp Wor on 5 Fig 58 Icon description overview Operating Instructions HERAcell 150 i 240i 7 Handling and control Gas cylinder empty Error message indicating that the filling level of one or of serveral gas cylinders is too low to ensure regular gas supply The monitoring function is available only for device versions with the optional gas monitoring system Request service This indicator shows that the routine service is due The display of the icon is controlled by the time entry in the REMINDER INTERVAL dialog box and it appears after the reminder message has been confirmed Gas cylinder filling level display optional If the device is equipped with the optional gas monitoring system the icons 1 for gas cylinder A and gas cylinder B appear in the corresponding CO3 display O display The icons indicate the gas cylinder filling level full empty The icon with the blue frame indicates the bottle not in use that will be switched over to continue the supply Manual changeover to a full gas cylinder is possible gt Press the cylinder icon with the blue frame or changeover can occur automatically if gt the cylinder pressure drops below 0 6 bar Af
83. display for the value required gt press the SHOW TREND ICON 1 37 0 Temp 3 6 m 9 C 2 To display an enlarged section gt Open a rectangular section 2 in the desired 8 size of the rectangle is determined by dragging a diagonal from the start press onto screen at left of upper diagram frame to the end release at right of lower diagram frame gt Press any position within the marked rectangle area 2 The section is now enlarged HERAcell 150 i 240 00 xxx 2008 00 00 gt This process can be repeated any number of times until the section is displayed in the desi red enlargement or until the maximal enlarge ment level max 30 event logger elements i e 30 min process at a logging cycle of 60 seconds gt During zoom mode the trend display can be scrolled forward and back 3 To return to the total trend gt Open rectangle over a small diagram section and press any position outside the marked area 4 To exit the trend display 3 gt Press the END key 3 The system returns to the main menu Fig 62 Trend display scaling NOTE Logging cycle The time interval of the data logging cycle can be redefined in the INTERVAL TIME EVENT LOGGER dialog box see chap 7 11 2 pg 83 50115191 07 2008 94 Operating Instructions HERAcell 150i 240 hermo 7 Handling and control 7 13 Error messages HERAcell 150 i 240 i 00 xxx 2008 00 00 Th
84. e b i z 10 0x30 reduce default value z 2 10 return 2 num 2 string char num 2 str int z char b number with one decimal place char a 12 char i 1 int rest 0 T C 0s Negative number if z lt 0 set algebraic sign b 0 1 35 convert value z Oxffffffff z 41 store decimal place rest z 10 143 Operating Instructions HERAcell 150 240 i 15 Annex Data communication Writing data in a file 50115191 07 2008 cut off decimal place z 2 10 calculate and copy number before decimal separator for i 0 i lt 12 i calculate value a i z 10 0x30 reduce default value ge ml Number copied completely if 2 0 break for i gt 0 i b l a i calculate and copy number after decimal separator b l calculate value b 1 rest 10 0x30 return 1 read_datalogger int read_datalogger define SIZE DATA2 16 define EVENT_STATUS 0x01 unsigned char buffer 300 string 300 unsigned char zahlenstring 150 datestring timestring unsigned char excelstring 150 unsigned char len h i unsigned int read count status define EVENT DATA END OxFF char data int GetTele 0 GetError 0 writing the title line in the file WriteFile FileHandle Date Time Comment Temp Act CO2 Act 02 Act rH Act Temp Set CO2 Set O2 Set rH Set n 85 infinite loop
85. e Stop CONTRA CON DECONTAMINATION ROUTINE dialog box 1 is displayed The decontamination routine must be stopped manually To stop contra con gt Press the END key 2 gt The system returns to the main menu NOTE Opening door during contra con decontamination If the door will be opened and closed again whilst contra con decontamination is running the routine returns to a phase which will ensure a continuation without faults 50115191 07 2008 109 HERAcell 150 i 240 i 00 xxx 2008 00 00 Stop decontamination routine D 1 Open door 2 Remove remaining water 3 Close door 6 If required run auto start routine Fig 67 contra con completion 150 i 240 i Thermo Operating Instructions HERAcell 10 1 Inspections and checks To ensure the operativeness and the operational safety of the device the functions and device components listed below must be checked at different intervals Daily check Gas supply of the CO supply system e Gas supply of the O2 N supply system Annual inspection Tightness of the glass door seal Permeability of the pressure compensation opening with insert Functional check of the operating panel and of the device control Electrical safety check in accordance with the relevant national regulations e g VBG 4 NOTE Functional check If safety devices were removed or disabled for inspections the device must not be operated before the
86. e control panel works as a touchscreen and can be controlled performing light pressure on the pressure sensitive areas of the screen using a finger or a blunt pin Temperature display 1 e CO display 3 e O display 5 optional contra con key 2 menu key 4 auto start key 6 NOTE Extended pressure sensitive area To accept a failure message the entire touchscreen can be used as a pressure sensitive area 50115191 07 2008 65 Fig 29 Power switch HERAcell 150 i 240 i 00 xxx 2008 00 00 Temp auto start Fig 30 Pressure sensitive areas Operating Instructions HERAcell 150i 240i 7 Handling and control Versions without O N control Fig 31 Function keys and value displays of the operating panel for a device version without O2 N gas supply 1 Type designation of the device 2 Temperature display 3 CO display 4 Key for starting the contra con decontamination routine b Key for opening the menu navigation 6 Key for starting the auto start routine 7 Actual value CO 8 Set value CO 9 Temperature actual value 10 Temperature set value 11 Current date and time Version with combined CO2 0O N control optional Fig 32 Function keys and value displays of the operating panel of a device version with combined CO4 O5 N gas supply 1 Type designation of the device 2 Temperature display 3 CO display 4 CO set
87. e error detection system is an integral element of the device internal control system It monitors the control loops and their sensors If an error is detected in the system the alarm relay switches and issues the following signals and messages e An audible alarm sounds e 8 flashing warning triangle 1 is displayed in the main menu the value indicators are no longer updated the detected error is listed in the error table the event is entered into the event display 7 13 1 Response to an error message event If the alarm relay was switched due to a user action the switching state can be reset by accepting the error HERAcell 150 i 240 i message e g for the manual cancellation of the contra con decontamination routine If the alarm relay was switched due to a techical Error defect the switching state remains active until the rH water defect has been corrected e g low water level in work space 1 To accept the failure message gt Press any position on the touchscreen gt The Error dialog box 2 apppears and the detected error is displayed gt the audible alarm is switched off 2 To exit the error display gt Press the END key 3 Fig 63 Event error messages gt The error message disappears 7 13 2 Troubleshooting The error tables indicate source of error cause of error and possible corrections For any communication with the Technical Support of Thermo Fisher Scientific please have
88. e turning device is packed in a separate box The boxes 1 to 4 are delivered with the device 50115191 07 2008 21 Operating Instructions HERAcell Location Technical ventilation 50115191 07 2008 150 i 240 i Thermo 3 1 Ambient conditions The device must only be operated at locations that meet the particular ambient conditions listed below Requirements Draft free and dry location The minimal distance to adjacent surfaces must be observed on all sides see Section 3 3 The operating room must be equipped with appropriate room ventilation Solid level fire proof surface Vibration proof substructure floor stand lab table capable of bearing the weight of the device and of accessories particularly if several devices are stacked The electrical system of the device has been designed for an operating height of up to 2000 m above sea level To ensure a constant incubation temperature of 37 C the ambient temperature must be within a range of 18 C to 33 C Relative humidity up to 8096 max No direct exposure to sunlight Devices that produce excessive heat must not be placed near the location of the HERAcell 150 i 240 i 3 2 Room ventilation When CO O N is supplied the work space of the incubator is slightly pressurized The pressure is released through the pressure compensation opening into the operating room Pressure compensation and any opening of the g
89. ece eee eee ee aeeaeee ai aa nnne nennt enn 2 3 Standard equipment components HERAcell9 150 9 6 9 95 695 55 2 4 Optional equipment components HERAcell 150 9 9 9 6 9 95 95 95 55 2 5 Standard equipment components HERA cell 240 6 9 95 9 5 95 5 9 2 6 Optional equipment components HERA cell 240 6 5 95 6 9 95 55 95 5 3 Installation of the device 3 WAMBISNECOMGITIONS cm 3 2 ROOM ventilation aoa t PIE 3 3 Space requir maents nocere ihr Ganka dne sk deg eg 8 N SNR 4 8 SI cepe D 3 5 SLACKING s citi ridge de 3 6 Retrofitting Modifications nennen nnne nennen nnn 4 Description of the device 4 1 Front view of HERAcell 150 1scocacscasccucecsecnursdarscatadedictu 55 et atat ta 9 4 2 Rear view HERAcell 150 i tentent rentes 4 3 Front view HERAcell 240 I3 1 apa 8 bnc 4 4 Rear view HERAcell 240 i sssssssssesssesseessesssecssesssessecsuesssessscssecssesssesseesveesvennsenes 45 Safety devices iue ridere canet vxor Ee 4 6 Work space atmosphere sse 8 4 4458 4 7 IDOOF SWIICI 0 a a 4 8 Sensor s
90. econtamination 125 15 Annex Data communication 126 15 11 InterfaGes iere cte spac 126 15 1 1 85 232 16 88 6 0 99 5529559 99 a aE Ard 126 15 1 2 USB port Optional oo cece eee iaaa ia E nnne 126 15 1 3 Installing the USB port driver 5 eee 5445455553 4 nnn 127 15 2 Structure of the command sequences for data communication 129 15 2 1 Description of the 6 010601 5559544 4445355355 4 444 129 15 3 Overview of general parameters addresses Oxxx 130 15 3 1 Reading general parameters ssssssessesseeeenneeeennn enn 130 15 4 Overview of incubator parameters addresses 2xxx cessus 131 15 4 1 Reading basic parameters ssssssssssssesesee eee 131 15 4 2 Reading internal function parameters ssssseeeneeeenrees 132 15 5 Error memory structure ssssssssssssseeenee tenentes nennen nnne 134 Operating Instructions HERAcell 150 i 240 i li hermo 15 5 1 Error memory data set structure 5 5 9 135 15 5 2 Overview of the possible error messages in hex coding
91. eeve 3 All process gases must be supplied to the device at a fixed pressure that has been preset within a range of 0 8 to 1 0 bar and must remain unchanged Before the gases are fed into the work space they flow through a gas inlet filter with a separation rate of 99 97 for a particle size of 0 3 um HEPA filter quality The illustration shows the optional combined gas connection without the optional gas monitoring system Label The label 4 contains information about gas supply an alarm contact terminal legend and notes about the electrical fusing of the device RS 232 interface Via the RS 232 interface 5 the incubator can be connected to the serial interface of a PC This connection allows the computer aided acquisition and documentation of the major operating parameters temperature CO5 O5 N5 concentration error codes etc USB interface optional As an alternative to the RS 232 interface 5 the incubator can be connected to a PC via the optional USB interface 6 This connection USB 1 1 USB 2 0 full speed compatible allows the computer aided acquisition and documentation of the major operating parameters temperature CO O gt N gt concentration error codes etc 50115191 07 2008 42 Fig 12 Supply interface Operating Instructions HERAcell 150i 240 hermo 4 Description of the device External alarm system 50115191 07 2008 Alarm contact Fig 12 The dev
92. el to the rear panel of the device The position to the right side wall is predetermined by the hose length 1 Install hose 2 to the sleeve of the gas humidification and then to the sleeve 3 of the device integral oxygen or nitrogen supply line 2 Place gas humidification immediately to the rear panel of the device and align it parallel to the rear panel 50115191 07 2008 Fig 19 Installing gas humidification Operating Instructions HERAcell 5 4 Installing the optional bottle turning devices HERAcell 240 i 150 i 240 i Thermo Align the roller insert so that the roller holder 8 and the crossmember 7 are flush and facing the work space opening 2 Slide the roller insert with both guide rails 5 onto the shelf supports 3 Slightly raise the roller insert so that the withdrawal stop can be routed over the shelf support 4 Insert the drive roller 6 into the right receptacle and connect the cable 4 to the corresponding control unit a 5 Insert the four idle rollers 3 the into receptacles and position the rollers in the receptacles in accordance with the bottle diameter 6 Place the bottles onto the rollers To ensure that Fig 20 Installing the bottle turning devices the bottles will not be shifted against each other by the rotation the bottoms of the bottles should contact the stop disks 2 of the rollers and the cylinder necks 1 should contact the rubber stop rings 8 The
93. ell 150 i 240 i 00 xxx 2008 00 00 a D Interval time data logger current 60 Loos 60 2 3 4 Fig 49 Logging cycle setting Operating Instructions HERAcell 150 i 240i 7 Handling and control Fig 49 The settings can be made within the value range of 10 seconds to 3600 seconds 1 To increase the value gt Press the key 4 2 To reduce the value gt Press the key 2 3 The value change appears in the display 1 4 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Event Logger menu NOTE Event logger interval time The logging cycle time does not affect the entries of the error table Displaying the error table The error table lists the errors detected by the device integral monitoring system in descending chronological order The most recently detected error is listed at the top position of 22 possible entries An entry consists of the control loop in which the error occurred the date the time and an error description The error table can be displayed but not edited If the event display consists of two pages the user can browse through the list The status indicator 2 indicates which page of the two is currently being displayed 1 To browse forward in the error table gt Press the CONTINUE key 4 2 To browse backward in the list gt Press the PREVIOUS key 3 To exit the display gt Press the END key 3 g
94. entation The routine is divided into five phases 1 Heating phase 2 decontamination phase 3 condensation 4 cool down 5 drying Heating phase Remaining run time approx 25 hours The work space is heated to a temperature of 90 C while an elevated relative humidity is created Decontamination phase Remaining run time approx 23 hours After the decontamination atmosphere has been created the decontamination phase of approx 9 hours is started The temperature is maintained at 90 C Condensation Remaining run time approx 14 hours The floor heating is switched off the temperature is maintained only by the sidewall heating This function is activated to reduce the created moisture before the cool down and to collect it at the work space floor Cool down phase Remaining run time approx 8 hours The device cools down until the originally set temperature set value is reached Postheating phase Remaining run time approx 1 hour During the postheating phase condensate within the device is eliminated as far as possible remaining condensate accumulates at the bottom of the work space End of the decontamination routine Remaining run time 0 hours When the remaining run time has elapsed to 0 hours the device has reached the originally set working temperature again e g 37 C The contra con decontamination routine must then be stopped by pressing the appropriate key 50115191 07 2008 Heating
95. erating room must be at least 18 C and the incubation temperature must exceed this temperature by at least 3 C The heating system controls the incubation temperature from this temperature threshold up to 55 C The principle of air jacket heating and the additional separate heating of the outer door and glass door gas tight screen minimize the risk of condensate formation at the side walls at the ceiling of the work space and at the glass door gas tight screen Relative humidity The heating of the work space promotes the condensation of the water thereby ensuring a constant humidity within the work space For the running operation always keep a sufficient quantity of processed water of the following quality available HERAcell 240 i quantity 4 5 I e HERAcell 150 i quantity 3 0 Quality characteristic demineralized and either destilled or autoclaved for sterilization or completely deionized and either destilled or autoclaved for sterilization Under normal operating conditions and at the usual incubation temperature of 37 C a constant relative humidity of approx 93 is 38 Operating Instructions HERAcell CO Work space atmosphere control O2 Work space atmosphere control N Work space atmosphere control 50115191 07 2008 150 i 240 i Thermo achieved in the work space If dew formation occurs on the culture containers due to elevated relative humidity the humidity in the work space can
96. evice the following set values have been preset Temperature 37 C e CO content 0 0 e O content optional 21 0 Optional bottle turningdevice speed a b c and d 0 NOTE CO O control Since the CO concentration of the air is nearly 0 the CO control and the control loop error monitoring system are disabled at a set value of 0 Since the O concentration of the air is nearly 21 the O5 control and the control loop error monitoring system are disabled at a set value of 21 7 4 Heat up phase of the control loop sensors When the device has been switched on the control loop sensors pass through a heat up phase of varying duration during the start process Temperature control loop 10s CO control loop 10s CO control loop with IR measuring cell 3 min O control loop 5 min The start process is indicated by an audible signal During the heat up phase the displays show dots instead of values Temperature display 2 e CO display 1 and Os display 3 After the heat up phase has been completed the control loop actual values are indicated 50115191 07 2008 68 contra con auto start Fig 34 Heat up phase display Operating Instructions HERAcell 150 i 240i hermo 7 Handling and control NOTE Exposure to CO gas During the 5 minute heat up phase of the 32 control loop the work space is not exposed to CO gas and the CO control and is disabled 7 5 Event
97. f the door switch is activated by opening the glass door the gas supply and the heating of the work space are interrupted and the display shows a corresponding message If the door remains open for more than 30 seconds a short audible alarm sounds If the door remains open for more than 10 minutes an audible alarm sounds and the alarm relay responds The outer door can only be closed after the glass door has been latched properly NOTE Versions with gas tight screen For devices with the optional gas tight screen the door switch function described above is activated when the outer door is opened Fig 10 Door switch 50115191 07 2008 40 Operating Instructions HERAcell 150i 240 4 8 Sensor system The blower wheel and two sensor modules are integral to the baseplate 1 of the measuring cell Sensor 2 for the acquisition of the work space temperature and the thermal protection e CO sensor 3 for the acquisition of the CO content in the work space atmosphere The O sensor optional 4 for the acquisition of the O content in the work space atmosphere is installed at various locations next to the measuring cell HERAcell 150 i in the sidewall in the upper area 5 HERAcell 240 i in the ceiling of the work space 4 The sensor for the acquisition of the work space temperature as well as the CO sensor and the O5 sensor are integral to the control system of the device Their measu
98. file 15 7 2 Example of a code for a a data logger query Creating a hexadecimal number char ahex char a char ahex char a char i char hexa 16 0123456789abcdef for i 0 i lt 16 i if a hexa il return i return 0 j Sending a telegram send telegramm void send telegramm char p char string 15 unsigned char bcc OxFF char i copy telegram together strncpy amp string 0 xxxx 00 00 r 14 insert 4 digit address strncpy amp string 2 p 4 50115191 07 2008 142 Operating Instructions HERAcell 150i 240 hermo 15 Annex Data communication Receiving a response telegram Creating a time format in ASCII Hexadecimal values in ASCII characters 50115191 07 2008 calculate checksum inverted XOR of all bytes without checksum and lt CR gt for i 0 i lt 11 i bee bcc string il copy checksum string 11 hexa bcc 16 string 12 hexa bcc 16 send telegram ComWrt COM_NR string 14 return get_telegramm int get_telegramm char p int reading_count 0 reading the telegram character by character do ComRd COM NR amp p reading count 1 until reception of CR while p reading_count r return number of received characters return reading count time_2_str char time 2 str int z char b char i output two numerals for i 1 3 gt 0 i calculate valu
99. g on the requirements to the work process the 33 control can be switched on and off This setting is possible only on versions with the optional O N5 control 1 To toggle between two states of the O control gt Press the key 4 or gt Press the key 2 2 The value change appears in the display 1 3 To accept and save the setting gt Press the ENTER key 3 gt The system returns to the Options menu NOTE 02 value display If the O control has been switched off the 02 display does not show an actual value x This procedure provides protection for the 02 sensor If the set value is set to 21 the 32 control loop is not monitored This applies to both O control ranges Control range 1 1 10 21 Control range Il 5 to 90 In this case the O display shows the actual value Airing the work space If the device was operated with O5 or N2 the work space must be aired after the O control has been switched off 50115191 07 2008 89 Menu gt Options gt o2 HERAcell 150 i 240i 00 xxx 2008 00 00 O2 configuration current on fat off 2 3 4 Fig 57 Switching the 32 control on and off Operating Instructions HERAcell 150 i 240i 7 Handling and control Gas monitoring The switching state of the O5 control loop does not affect the function of the optional gas monitoring system The gas monitoring system remains active even when the O
100. he device fuses Fig 74 The two identical device fuses 4 are installed in the fuse compartment 1 next to the power plug receptacle of the device Time delay fuses 6 3 A 5x20 mm The fuse holder is secured to the fuse compartment 1 using two locking tabs 2 2 To remove the fuse holder squeeze the two locking tabs and pull the holder 3 out of the fuse compartment 3 Remove the faulty fuse from the fuse holder and install the new fuse 4 Slide the fuse holder into the fuse compartment and press the holder on until the locking tabs are fully engaged 50115191 07 2008 116 4 Fig 73 Gas inlet filter installation Fig 74 Device fuse replacement Operating Instructions HERAcell 150i 240 hermo 10 Maintenance 10 9 Replacing the door seal 1 2 The door seal magnetic seal 3 of the outer door is SERM located in the retaining slot No tools are required to replace the seal 1 Pull the magnetic seal 3 out of the guide slot 1 2 Position the new seal at a corner 2 and press the seal retaining rail 4 into the slot 3 Make sure that the retaining rail taper is 3 positioned correctly in the slot 1 and that the seal is flush with the door frame 166 Fig 75 Magnetic door seal replacement 50115191 07 2008 117 Operating Instructions HERAcell 150 i 240 hermo 11 Disposal Contamination hazard The device may have been used for treating and pro
101. ice can be connected to an on site external alarm system e g telephone system building monitoring system visual or audible alarm system For this purpose a potential free alarm contact 7 is preinstalled in the device NOTE Alarm contact The alarm contact responds for all errors reported by the control loops see chap 7 13 pg 95 Power supply connection Fig 12 The connection to the power supply system is established by connecting a cable with plug for IEC connector 9 to the socket 8 The receptacle for the two device fuses is integral to the socket 43 Operating Instructions HERAcell 150i 240 4 10 Work space components The work space of the incubator has only a minimum of surface thereby supporting both the prevention of contamination and easy effective decontamination 4 10 1 Interior container All components of the work space are made of stainless steel and have a high gloss polished absolutely smooth and easy to clean surface Any embossings have a large radius As an option the interior container the shelf system and the blower wheel with its cover can be made of copper material NOTE Oxidation of copper components When exposed to heat and humid air the copper material of the interior container will oxidize This results in a discoloration of the copper components during the test run for checking the device functions Do not remove the oxide layer during routine cle
102. ich page of the total number of pages is currently being displayed 1 To browse forward in the list gt Press the CONTINUE key 5 2 To browse backward in the list gt Press the PREVIOUS key 3 3 To exit the display gt Press the END key 4 gt The system returns to the Event Logger menu Changing the logging cycle time Due to limited memory resources the oldest entries are deleted as new entries are logged The period from which the displayed entries originate depends largely on the selected logging cycle time Logging cycle time 8s 89 Minimal displayed period The setting controls the logging cycle in sections of seconds during which the control loop measured values are logged during the operation of the device and displayed by the trend display see chap 7 12 pg 94 50115191 07 2008 Menu gt Event logging gt Event display 1 2 HERAcell 150 240 i 00 00 Date Time Process 002 004 09 05 08 10 20 13 04 08 11 15 10 03 08 11 17 10 03 08 11 12 10 03 08 11 02 09 03 08 12 03 09 03 08 11 00 09 03 08 10 40 auto start successfully completed Start auto Start Set value change T Set value change T Calibration T Power reset Set value change CO2 Set value change T 09 03 08 08 45 Set value change T 09 03 08 08 10 Power reset Previous End Continue 3 4 5 Fig 48 Event display Menu gt Event logging gt Recording Interval HERAc
103. ing cell For devices with infrared IR measuring cells the CO calibration can only be performed when the CO concentration has been set to 4 0 or more Calibration should be performed with the CO set value designated for the work process prospective work process value 50115191 07 2008 114 A m 3 m m E 1 2 Fig 71 Gas tight screen measurement opening Operating Instructions HERAcell 150i 240 10 Maintenance 10 6 CO calibration procedure Measurement example e CO set value 5 Reference temperature 5 6 1 Press the CO DISPLAY key 1 gt The CO menu is displayed 2 To exit the CO menu gt Press the END key 3 3 To enter the Calibration submenu gt Press the CALIBRATION key 2 4 To enter the measured value targeting value The targeting value can be increased or reduced in increments if you keep the key 4 or the key 6 depressed the function switches to a rapid increase reduction after approx 3 seconds another increase reduction occurs To increase the targeting value gt Press the key 6 To reduce the set value gt Press the key 4 5 To accept and store the targeting value gt Press the ENTER key 5 gt The system returns to the main menu The temperature display shows the current actual value measured in the CO display NOTE Excessive CO content Excessive CO content after the calibrat
104. ining disinfectants Chloride containing disinfectants can corrode stainless steel Useonly disinfectants that do not affect stainless steel 101 Operating Instructions HERAcell 150 i 240i hermo 9 Cleaning and disinfection 50115191 07 2008 Predisinfection Preparing the manual wipe spray disinfection Electric shock Contact with current carrying components may cause a lethal ill electric shock Prior to cleaning and disinfection work disconnect the device from the power supply Turn the device off using the power switch Unplug the power connector and protect it against accidental reconnection Check to see if the device is deenergized N Health hazard The surfaces of the work space may be contaminated Contact with contaminated cleaning liquids may cause infections Disinfectants may contain harmful substances When cleaning and disinfecting always observe the safety instructions and hygiene regulations e Wear safety gloves Wear safety goggles Wear mouth and respiratory system protection gear to protect the mucous membranes Observe the safety instructions of the manufacturer of the disinfectant and of the hygiene experts Oe Predisinfection 1 Remove all samples from the work space and store them in a safe place 2 Pump the water off and remove water residues using a cloth 3 Spray disinfectant onto the surfaces of the work space and of the accessories or wipe the su
105. ion can be reduced by leaving the device doors open for approx 30 seconds Value reset If the value isn t changed within the next 30 seconds the system automatically exits the menu and the most recently confirmed value is preserved 50115191 07 2008 HERAcell 150 i 240 i 00 xxx 2008 00 00 19 1 a co2 5 0 HERAcell 150 i 240 i 00 xxx 2008 00 00 Show progress B ii 2 3 HERAcell 150i 240 i 00 xxx 2008 00 00 CO2 calibration current Fig 72 CO calibration procedure Operating Instructions HERAcell 150i 240 10 7 Replacing the gas inlet filter Fig 73 The gas inlet filter CO2 O5 N supply has plastic threads and is screwed by hand into the threaded hole at the control box Procedure for gas supply gas inlet filter 1 Make sure that the gas supply is shut off 2 Loosen the hose clamp 4 3 Remove the gas hose 5 from the sterile filter sleeve 2 Procedure for all gas inlet filters 4 Remove the retainer 1 5 Unscrew the gas inlet filter 2 from the threaded hole 3 6 When installing the new gas inlet filter make sure that the plastic thread is not canted Install the filter using caution 7 Install the retainer 1 Procedure for gas supply gas inlet filter 8 Connect the gas hose to the gas inlet filter sleeve and secure it using the hose clamp Check to see if the gas hose is securely seated on the sleeve 10 8 Replacing t
106. is installed in place of the glass door door hinge replacement on outer door and glass door lockable outer door split inserts infrared IR sensor PCB for USB interface NOTE Modifications Retrofittings and modifications must only be performed by the Technical Service of Thermo Electron LED 25 Operating Instructions HERAcell 150i 240 li hermo 4 Description of the device 4 1 Front view of HERAcell 150 i Front view 1 Stacking elements 2 Plug caps 3 Glass door 4 Measuring cell with blower wheel and sensors 5 Door switch 6 Oxygen sensor optional 7 Pressure compensation opening with insert 8 Measuring opening 9 Outer door 10 Outer door seal replaceable 11 Stand height adjustable 12 Gas humidification optional 13 Water level sensor 14 Nameplate 15 Power switch 16 Support rail 17 Shelf 18 Latch glass door 19 Support hook for shelf 20 Access port with plug 21 Glass door seal replaceable 22 Operating panel touchscreen 23 Door handle 50115191 07 2008 26 Operating Instructions HERAcell 150 240 i li hermo yess 1 FSS 16 WF co X 15 rj 2 14 Fig 4 Front view HERAcell 150 i 50115191 07 2008 27 Operating Instructions HERAcell 150 i 240i 4 Description of the device 50115191 07 2008 Rear view 4 2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Rear
107. isplayed as a security request see above Proceed with work step 2 see section above 9 4 3 contra con interruption due to error If an error occurs while the decontamination routine is run an error message 3 is displayed and the following actions are initiated The decontamination routine automatically changes to the cool down phase the audible alarm sounds 1 To confirm the audible alarm gt Press any position on the display The audible alarm is switched off The END key is displayed If the decontamination routine is not cancelled after this cool down to the set temperature occurs 2 To cancel contra con gt Press the END key 2 gt The error message is displayed gt If the error message is confirmed the system returns to the main menu 50115191 07 2008 HERAcell 150 i 240 i 00 xxx 2008 00 00 contra con Stop lt End gt Stop dekontamination routine lt Back gt Continue qe 1 2 HERAcell 150 i 240 i 00 xxx 2008 00 00 contra con Status 1 Temperature 37 C Start time 14 00 Phase Heat Time remaining 25 00 HERAcell 150 i 240 i 00 xxx 2008 00 00 contra con Error D Temperature 37 C Start time 14 31 Phase Cool Time remaining 1 00 Fig 66 Interrupting cancelling contra con 108 Operating Instructions HERAcell 150i 240 9 Cleaning and disinfection 9 4 4 Completing contra con After the five phases have been completed th
108. ith the latest technological developments and is operationally safe However the device may present potential hazards particularly if it is operated by inadequately trained personnel or if it is not used in accordance with the intended purpose Therefore the following must be observed to prevent accidents Target group The CO incubator must be operated only by trained and authorized personnel Process procedures for personnel For any operation of this device the operator must prepare clear and concise written instructions in the language of the operating and cleaning personnel based on these operating instructions applicable safety data sheets plant hygiene guidelines and technical regulations in particular which decontamination measures are to be applied for the CO incubator and accessories which protective measures apply when specific agents are used which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel 50115191 07 2008 10 Operating Instructions HERAcell 150 i 240 i 1 General notes 1 3 Applicability of the instructions Original document for translation Requirements for warranty 50115191 07 2008 The contents of the operating instructions are subject to change without further notice Concerning translations into foreign languages the German version of these operating instructions is binding
109. l oxygen or nitrogen supply line 4 The inflowing oxygen or nitrogen is fed to the heated water This ensures humidification of the gases as they enter the work space and prevents an undesired drop of the work space humidity 4 10 5 Heating system An air jacket heating is used for heating the work space The arrangement of the heating elements ensures that condensate formation above the water reservoir is prevented as far as possible The outer door of the device is also heated The heat radiated onto the interior glass door gas tight screen prevents condensate formation The work space of the device always remains visible despite high humidity 50115191 07 2008 Fig 14 Water reservoir Operating Instructions HERAcell 150i 240 4 10 6 Rear panel openings A pressure compensation opening with insert in the rear panel of the device allows a compensation between the pressures in the work space and in the operating room A sealable access port allows cables hoses or additional sensors to be routed into the work space of the device HERAcell 150 i access port 42 mm 1 pressure compensation opening 2 HERAcell 240 i access port 42 mm 3 pressure compensation openingt 4 NOTE Operating conditions When accessories are to be operated in the work space of the CO incubator the ambient condition requirements must be observed see table below The energy introduced into
110. lass door gas tight screen during the operation of the device will release very small quantities of CO5 2 into the operating room the room ventilation must be capable of carrying the released gas safely off into the open In addition heat dissipating from the device during continuous operation may cause a change in the room climate Therefore the HERAcell 150 i 240 i must only be installed in rooms with sufficient ventilation Do not install the device in room recesses without ventilation The room ventilation should be a technical ventilation that complies with the requirements of ZH 1 119 Guidelines for laboritories Germany or some other suited ventilation system with appropriate capacity 22 Operating Instructions HERAcell 150 i 240i 3 Installation of the device 3 3 Space requirements Fig 1 When installing the device make sure that the installation and supply connections are freely accessible The control box on the rear panel of the device may serve as a spacer to adjacent objects The side distances given are minimal distances To protect the CO incubator against contamination use a floor stand even if the device is installed near the floor The height of the floor stand should not fall below 200 mm NOTE Accessibility of the devices To ensure the accessibility for care and maintenance works keep larger side and rear distances 50115191 07 2008 23 SCIENTIFIC
111. ller set value The value is determined using a diagram see chap 5 4 pg 51 50115191 07 2008 48 Operating Instructions HERAcell 150i 240 5 1 Preparing the work space 8 Upon delivery the CO incubator is not in a sterile state Before the initial start up the device must be decontaminated Before the decontamination is performed the following work space components must be cleaned Support rails support hooks shelves bottle turning assembly optional gas humidification optional work space surfaces work space seals and gaskets glass door gas tight screen NOTE Decontamination For details about the cleaning and disinfection of the device see chap 9 pg 100 5 2 Installing the shelf system Tools are not required for the installation of the shelf system The support rails are secured using spring pressure After the support hooks have been inserted into the rail the shelves are pushed onto the support hooks 5 2 1 Installation removal of the support rail The support rails are held at the sides by the embossings 2 and 5 and secured by the embossings 1 and 6 The support rails marked with the diamond shape are inserted atthe rear panel of the device with the locksprings 3 facing upward 1 Position the support rail 4 onto the lower embossing 6 and tilt it toward the work space side wall so that the rail is positioned over the two embossings 5 an
112. loops or other parameters can be entered byte 8 15 or ASCII character 16 31 Example of a response l 2400 e0 010b01060f37000001 770028004400001 10b01060f38000001 72003200420352 80 First data set 2400 e0 010b01060f3700000177002800d4000011 consisting of 32 byte ASCII characters Second data set 0b01060f3800000172003200d20352 80 beginning of the second data set after 32 bytes of the first data set 138 Operating Instructions HERAcell 50115191 07 2008 150 i 240 i Thermo 15 6 1 Data logger data set structure scheme 01 Ob 01 06 Of 37 0000 0177 0028 00d4 0000 Data logger entry type 0x01 Standard entry that is stored every 60s 01 Date Day 0x0b 11th day of the month Date Month 0x01 January 01 Ob Date Year 0x06 2006 06 Time Hours OxOf 15 hrs Of Time Minutes 0x37 55 minutes 37 Device status 0x0000 Everything OK Type specific here current temperature in 1 10 Celsius 0x0177 37 5 C Type specific here current CO2 content in 1 10 0x0028 4 0 CO Type specific here current O content in 1 10 0x00d4 21 2 O Reserved for rh in 1 10 always 0x0000 0 0000 0177 0028 00d4 0000 Therefore the following information is transmitted in this data set Created on 1 January 2006 at 15 55 hrs The device status reports no particularities the temperature is 37 5 C gas con
113. m are disabled at a set value of 21 The control loop error monitoring system optional is enabled 50115191 07 2008 72 HERAcell 150i 240 i 00 xxx 2008 00 00 HERAcell 150i 240 i 00 xxx 2008 00 00 Show trend B pik E 21 9 2 3 HERAcell 150 i 240 i 00 xxx 2008 00 00 O2 change set value current 21 0 21 0 amp 4 5 6 Fig 37 Setting the O set value Operating Instructions HERAcell 150 i 240 i Thermo 7 9 Auto start routine The auto start function is an automated routine for the start and the subsequent adjustment of the CO measuring system After the start the device control adjusts the temperature to the set value while humidity is generated When temperature and relative humidity have reached constant values the CO measuring system is automatically adjusted to these values and the work space is supplied with the preset quantity of CO3 NOTE Application of the auto start routine To ensure that the specified accuracy of the CO measuring system is maintained the device should always be started using the auto start routine if adifference of more than 1 C is entered upon setting the temperature set value the Low Humidity function is enabled disabled the device is restarted after an extended interruption of operation The auto start routine should be run at least every three months on the occasion of cleaning and maintenance
114. monitoring system The gas supply from the gas supply system to the device is established using the supplied flexible gas pressure hoses see chap 5 6 1 pg 55 CO connection e For a device with CO connection connect the gas supply to the sterile filter 2 in accordance with the connection diagram 1 Combined CO and 02 15 connection optional For a combined CO 5 O5 N5 connection proceed 85 follows in accordance with the connection diagram 3 e Connect the O2 N supply line to the upper gas inlet filter 4 e connect the CO supply line to the lower gas inlet filter 5 NOTE Manual gas monitoring On devices without the optional gas monitoring system there is no automatic gas monitoring Therefore the filling level of the gas bottles must be checked every day 50115191 07 2008 56 Fig 24 Gas connection without gas monitoring system Operating Instructions HERAcell 150i 240 5 6 3 CO connection with gas monitoring system optional The gas supply from the gas supply system to the device is established by the supplied flexible gas pressure hoses Fig 25 Devices with CO connections that are equipped with the optional gas monitoring system are connected according to the connection diagram 7 CO connection For a device that is operated with process gas CO3 and equipped with the optional gas monitoring s
115. n format xxxxxxxx Read bottle turning device 8 bytes hexadecimal 2133 status all levels 1 active O y value in format xxxxxxxx stopped 2 2140 Read status of gas cylinder 2 bytes hexadecimal changeover switch for CO 3 value in format xx Read status of gas cylinder 2 bytes hexadecimal 2141 changeover switch for O 3 value in format xx Up to 241 byt hexadecimal Read error memory current in Vice hexadecima 2300 value format see errors 4 s corresponding chapter Up to 241 bytes hexadecimal Read error memory prior 2301 value format see errors 4 corresponding chapter Query start of the data stored Up bytes hexadecimal 2400 value format see in the data logger 5 corresponding chapter 224 byt hexadecimal Query start of the further data vie 2401 value format see stored in the data logger 6 corresponding chapter 224 byt hexadecimal Repeated query of the most 2203 recent data logger query 7 value format s e gger query corresponding chapter 2410 Read data logger write cycle in 8 bytes decimal value in hours minutes seconds format xx xx xx 1 See table with notes on disinfection and autostart run status 2 2 bytes per level each 3 Bottle A active 0x01 bottle B active 0x02 bottle A pressure OK 0x10 bottle B pressure OK 0x20 4 For more information on the error memory see chapter 13 5 5 Set reading pointer to first entry
116. nding icon 5 is displayed in the main menu 50115191 07 2008 86 Menu gt Options gt Low humidity HERAcell 150 i 240 i 00 xxx 2008 00 00 Low humidity off on current neu 2 3 4 HERAcell 150 i 240 i 00 xxx 2008 00 00 36 9 Temp s 219 ena Fig 52 Low humidity setting Operating Instructions HERAcell 150i 240 7 Handling and control Setting the gas tight screen As the aperture cross sections are smaller when accessing the samples devices equipped with the optional gas tight screen achieve shorter recovery times for the incubation parameters e Work space temperature e CO concentration e O concentration e relative humidity After conversion of the device the device control system must be adjusted to the gas tight screen option NOTE Malfunction The conversion to gas tight screen causes a change of the control parameters Failure to set the gas tight screen function in accordance with the actually installed door version may impair the incubation performance 1 To toggle between two options 1 gt Press the key 4 or gt Press the key 2 2 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Options menu Switching the water level sensor on off For incubation operation with ambient humidity or if the auto start routine is to be run dry without water
117. ng len len time 2 str 0 amp excelstring len excelstring len switch zahlenstring i SIZE_DATA2 1 case EVENT_STATUS check cyclic entries for device errors status zahlenstring 6 i SIZE_DATA2 0x100 zahlenstring 7 i SIZE_DATA2 if status amp INFO_ERROR str_cpy amp excelstring len Error active 13 len 13 else query all device errors see chap 15 6 2 pg 140 if status amp DOOR_LONG str_cpy amp excelstring len Door open too long 19 len 19 else if status amp DOOR_OPEN str_cpy amp excelstring len Door open 10 len 10 query remaining device errors now d and finally query cyclic nominal values entries without device errors 145 Operating Instructions HERAcell 150i 240 li hermo SCIENTIFIC 15 Annex Data communication else str_cpy amp excelstring len ok 3 len 3 copy nominal values from numerical string to Excel string len num_2_str zahlenstring 8 i SIZE_DATA2 0x100 zahlenstring 9 i SIZE_DATA2 amp excelstring len excelstring len str zahlenstring 10 i SIZE_DATA2 0x100 11 i SIZE_DATA2 amp excelstring len excelstring len len num 2 str zahlenstring 12 i SIZE_DATA2 0x100 1 e s len num 2 zahlenstring zahlenstring
118. nnection optional CO and O5 N5 with gas monitoring optional Distributor connection for looping through the CO gas supply for up to three additional devices independent of device type Distributor connection for looping through the O5 N gas supply for up to 3 additional devices independent of device type Tee joint for fitting the gas pressure hoses together Gas pressure hose for looping through the gas supply Diagram Gas connection CO and O5 N5 with optional gas monitoring HERAcell 150 i with looped through gas supply therefore device must only be equipped with combined gas connection optional CO3 and O 4 N5 30 Operating Instructions HERAcell 150i 240 li hermo 4 Description of the device HERAcell 2 4 OJN Fig 6 Looped through gas supply HERAcell 150 i 50115191 07 2008 31 Operating Instructions HERAcell 150i 240 li hermo 4 Description of the device 50115191 07 2008 Front view 4 3 Front view HERAcell 240 i 1 Stacking elements 2 Plug caps 3 Glass door 4 Measuring cell with blower wheel and sensors b Door switch 6 Oxygen sensor optional 7 Receptacle for bottle turning device optional 8 Measuring opening 9 Plug for bottle turning device optional 10 Outer door 11 Outer door seal replaceable 12 Stand height adjustable 13 Drive roller for bottle turning
119. onfirm the input gt Press the ENTER key 3 gt The system returns to the Configuration menu NOTE Changing an existing code The currently valid code can be redifined in the KEYPAD Lock CODE dialog box of the SETTINGS SETUP menu see chap 7 11 3 pg 85 Resetting the code If the keypad lock code is no longer available the code must be reset to the standard code by the Technical Support of Thermo Fisher Scientific 50115191 07 2008 92 1 00 xxx 2008 00 00 HERAcell 150 i 240 i Fig 60 Enabling disabling the keypad lock Operating Instructions HERAcell 150i 240 li hermo 7 Handling and control 7 11 6 Software versions Menu gt Software versions This menu shows the unit software versions in the display 1 1 To exit the display gt Press the END key 2 gt The system returns to the Configuration menu HERAcell 150 i 240 i 00 xxx 2008 00 00 Software main board 50111927 Software meas cell 0027 Software display 50111388 Configuration 1490 Fig 61 Software versions 50115191 07 2008 93 Operating Instructions HERAcell 150 i 240i hermo 7 Handling and control 7 12 Scaling the trend display The trend display of the three control loops HERAcell 150 240 i Te Tar Temperature L 02 I N Show trend B e 02 can be scaled to two different versions ie 1 Full screen display L te gt Press the
120. ot available Faulty cancellation of the auto start routine The auto start is cancelled if anerror is detected in the temperature control loop e an error is detected in the CO control loop the water filling level is insufficient auto start Dry execution of auto start routine If the auto start routine is to be run dry without water in the floorpan of the work space the water level sensor must be disabled prior to its start see chap 7 77 3 pg 85 74 Operating Instructions HERAcell 150i 240 7 Handling and control 7 9 1 Activating auto start Preparations for the start 1 Make sure that the COs Os N gas supply system valves are open 2 Fill the floorpan of the work space with a sufficient quantity of water The filling level must not exceed the upper filling level mark 3 Set the set values for temperature CO and O5 on the touchscreen auto start routine activation 1 Press the auto start key 2 gt The auto start instruction menu 1 is displayed 2 To exit the auto start instruction menu and cancel auto start gt Press the END key 4 3 Activate the auto start routine gt Press the START key 3 To air the work space open both device doors 5 When the audible alarm sounds after 30 seconds close both device doors gt The trend status indicator 5 is displayed 6 During the progress the status indicator displays the following information Temperature
121. ouble click on the file SETUP EXE to open this file Desktop E Eigene Dateien E amp Arbeitsplatz E e System C E HERAcell_v01 El Driver ONES DE DES FR GB or 2 Locate the installation directory for the program VE HERAcell 150 amp 240 Destination Directory Salact the primary instalation directory Programme HERAcal 150 amp 240 3 In the sequence of the given installation steps confirm the licence agreement confirm the extent of installation after the message on the completeness of the installation has been displayed close the installation surface and restart the computer 50115191 07 2008 148 Operating Instructions HERAcell 150i 240 hermo 15 Annex Data communication 15 8 2 Operating the Heracell 150 i amp 240 i User menu structure The user surface is divided into two main menus MAIN with two functional elements Program version output FIRMWARE VERSION Switch for exiting the program QUIT HERfcell 150 240 COM NoCOM Piim x MAIN GENERAL HERAcell 150i amp 240i V 0 1 ThermoFisher SGCUEINAIMBRBRI E 50112431 28 05 2003 01 07 2008 MEN Lo 50111927 Submenus GENERAL with the submenus PRESETTING for setting the transmission speed and for selecting the serial port TEST COM for testing the communication connection between PC and incubator DATE amp TIME for adjusting date
122. pplicational hints and useful information 1 5 2 Additional symbols for safety informations Wear safety gloves Wear safety goggles Harmful liquids Electric shock Hot surfaces Fire hazard A e E D m Explosion hazard Suffocation hazard Q 50115191 07 2008 12 Operating Instructions HERAcell 150 i 240 i hermo 1 General notes 50115191 07 2008 C gepr fte Sicherheit 1 5 3 Symbols on the device CE conformity mark confirms conformity according to EU Guidelines VDE test mark Mark of conformity USA Canada Observe operating instructions 13 Operating Instructions HERAcell 150 i 240 i I hermo Use Fields of application Gas supply system Incorrect use 50115191 07 2008 1 6 Intended purpose of the device 1 6 1 Correct use The CO incubator HERAcell 150 i 240 i is a laboratory device for preparing and cultivating cell and tissue cultures The device allows the simulation of the special physiological ambient conditions for these cultures due to the exact control of e temperature CO content O N gt content and increased relative humidity HERAcell 150 i 240 i has been designed for installation and operation in the following fields of application Laboratories for cytobiological and biotechnological experiments of safety levels L1 L2 and L3 Medical microbiological laboratories in accordance
123. pression DIN VDE mefeemeiweN m lt 1 2 8 230 V AC Rated current A 5 4 120 V AC 6 3 100 V AC On site fusing Fuse Circuit breaker Rated input 0 65 120 V AC 0 63 100 V AC kW 0 64 230 V AC kW kW 50115191 07 2008 123 Operating Instructions HERAcell Please list carried out works here DeWeWe IBS 8 2 256 gt SeweemmBer location 66 Work carried out pep ee fo a a cP J EE 150 i 240 i Thermo 50115191 07 2008 124 Operating Instructions HERAcell Operating structions Mme 1501 2001 Thermo SCIENTIFIC 14 Certificate of decontamination Invoice recipient Customer no Location Forwarding address Year of manufacturer KC Name of technician Appointed date order date Ordered by Order no Type of device ID no Order no Operating hours Certficate of decontamination Dear customer when using biological and chemical agents within and ouside of devices hazards to the health of the operating personnel may be pre
124. r Suffocation hazard Large amounts of N released into the room atmosphere may cause suffocation If N is released initiate safety measures immediately Leave the room immediately and do not allow others to enter the room Inform security service or fire department Operating Instructions HERAcell 150 240i 2 Delivery of the device 2 1 Packaging Packaging materials 50115191 07 2008 Damage report The CO incubator HERAcell 150 i 240 i is delivered in a stable packaging box All packaging materials can be separated and are reusable Packaging carton Recycled paper Foam elements Styrofoam CFC free Pallet Untreated wood Packaging film Polyethylene Packaging ribbons Polypropylene 2 2 Acceptance inspection After the device has been delivered check the delivery immediately for completeness possible damage If components are missing or damage is found on the device or the packaging esp damages caused by humidity and water please contact the linehauler as well as the Technical Support of Thermo Scientific immediately 18 Operating Instructions HERAcell 150 2401 i hermo 2 3 Standard equipment components HERAcell 150 i HERAcell 150i Quantity of the delivered components CO incubator or CO O incubator continuous shelves continuous shelves Sp 8 1 3 9 lt 4 4 C Sheitsupponstorshef O 5 1 5
125. r water 3 Remove the cleaning liquid from the water tray and wipe all surfaces of the work space thoroughly dry 4 Wipe accessories thoroughly dry Final disinfection 1 Spray disinfectant again onto the work space the shelf system and the removed components and wipe them clean 2 Allow the disinfectant to react as specified by the manufacturer 3 Reinstall the shelf system and the removed components NOTE Functional check After the installation check to see if the blower wheel is securely attached to the axle and if it can rotate freely then secure cover using the screws 103 Operating Instructions HERAcell 50115191 07 2008 contra con process 150 i 240 i Thermo 9 4 contra con decontamination routine The entire program run of the decontamination routine takes approximately 25 hours During this routine a hot and humid atmosphere at 90 C with highly decontaminating effect is created in the work space The effectiveness of the contra con decontamination routine has been certified by independent institutes Information on these tests is available from Thermo Scientific upon request After the run has been completed the device must be started up again using the auto start routine NOTE Conditions that prevent the start of the contra con decontamination routine The contra con routine cannot be started if one of the following failure conditions exists Temperature control loop Sensor b
126. read up to 7 entries max 6 Send the next 7 entries Set reading pointer automatically to next new entry read up to 7 entries max 7 Send entries of last telegram again Can be used after communication error has occurred 50115191 07 2008 132 Operating Instructions HERAcell 50115191 07 2008 150 i 240 i Thermo Note on 3 disinfection and autostart run status e Od AE Perform counter voltage adjustment 06 61 9 Doeme mop a set check Poor eem Poor 9 133 Operating Instructions HERAcell 150i 240 li hermo 15 Annex Data communication Error memory data set structure 50115191 07 2008 15 5 Error memory structure The error memory contains 22 error messages A query is responded with 11 data sets with a colon as separator and can be queried using the following command Query 2300 00 cc CR Reading the ast 11 error memory entries Query 2301 00 cc CR Reading the first 11 error memory entries These data sets consist of 11 byte and are encrypted in 21 ASCII characters prior to data transmission Example The byte 0x23 is converted into the ASCII characters 0x32 2 and 0x33 3 Byte 1 consists of 1 characters Bytes 2 11 consist of 2 characters Therefore a response consists of 1 10 x 2 21 data bytes plus separator A
127. reakage actual value above set value excessive deviation actual value below set value excessive deviation set value not plausible calibration values too high or too low sensor communication failure sensor parameter not plausible nocommunication with LM 75 Control loop for CO gas supply Nocommunication with sensor In this case the conta con key is dimmed and its function is not available Overtemperature protection If the overtemperature protection was enabled on the device the contra con decontamination routine cannot be started before the fault hes been corrected or reset No gas is supplied during the contra con decontamination routine on versions with the optional gas monitoring system If the error no gas occurs during the contra con decontamination routine the audible alarm is triggered The alarm can be confirmed by pressing on any position on the display In this case the contra con decontamination routine is not cancelled The alarm relay remains switched until the gas monitoring system detects a full gas cylinder again 104 Operating Instructions HERAcell 150i 240 li hermo 9 Cleaning and disinfection contra con procedure 50115191 07 2008 Optional bottle turning device Before starting the contra con decontamination routine all rollers must be removed from the incubator The electrical connection sockets must then be sealed with the protecting caps The
128. red values are compared to the selected set values Based on this data the control system controls heating and CO2 O2 N gt supply The blower intermixes the supplied gases and ensures an even temperature distribution within the work space The thermal protection has been preprogrammed at the factory and cannot be changed It protects the stored cultures from overheating If the set temperature is exceeded by more than 1 C the thermal protection responds and the work space temperature is automatically reduced to the selected set value so that the incubation process can be continued even in case of a failure Any response of the thermal protection will simultaneously trigger a visual alarm If the thermal protection is enabled an error message is issued the alarm relay responds If the error message is accepted the display shows the Overtemperature icon to indicate the response of the thermal protection and the temperature display is highlighted in red 50115191 07 2008 41 Fig 11 Temperature CO9 and O sensors Operating Instructions HERAcell 150i 240 4 9 Supply interface All supply connections are installed in the supply interface control box 1 in the rear of the device Gas connection The gas supply lines between the device and the gas supply system are connected using the supplied connecting hoses O5 N is supplied to the device through connecting sleeve 2 CO is connected to sl
129. rfaces clean using disinfectant Moisture sensitive components Do not spray disinfectant onto the CO sensor in the measuring cell baseplate or onto the O N sensor 4 Allow the disinfectant to react as specified by the manufacturer Removing accessories and shelf system 1 Remove gas humidification bottle turning device and or shelves then remove the entire shelf system from the work space For removal and installation of the shelf system please refer to Section 5 2 2 If required remove the blower wheel and its cover from the baseplate 102 Operating Instructions HERAcell 150i 240 hermo 9 Cleaning and disinfection 50115191 07 2008 Final disinfection of the measuring cell The wheel and the cover can be autoclaved Removing blower wheel and cover Electric shock Contact with current carrying components may cause a lethal electric shock Prior to removing the blower wheel switch the device off and disconnect it from the power supply system 1 Remove the two retaining screws of the cover using the supplied Allen wrench 3 mm and remove the cover 2 The blower wheel is secured to the axle by a set screw Remove the set screw using the Allen wrench 2 mm and pull the blower wheel off Cleaning the work space and accessories 1 Thoroughly remove dirt residues and deposits using a solution of tepid water and dishwashing agent 2 Wipe the surfaces clean using a clean cloth and plenty of clea
130. rmo 5 6 Gas connection NOTE Gas quality The gases must have one of the following qualities e Purity 99 5 min medical gas quality Overpressure The operating pressure of the gas applied to the device must not exceed 1 bar If the gas is supplied at a higher pressure the valves integral to the device may not close correctly and the gas supply control may be impaired Set the gas supply to a range between 0 8 bar min and 1 0 bar max and make sure that this pressure setting cannot be changed 5 6 1 Installing gas pressure hoses The gas supply from the gas supply system to the device is achieved using the supplied flexible gas pressure hoses 1 Connect the gas pressure hose 2 to the sleeve of the gas supply system 2 Remove the protective cap 3 from the sterile filter 3 Slide the hose clamp 1 onto the gas pressure hose and connect hose to the sleeve 4 of the sterile filter 5 4 Secure the gas pressure hose to the sleeve of the sterile filter using the hose clamp Fig 23 Installing gas pressure hoses NOTE Pressure compensation opening To ensure permanent pressure compensation the pressure compensation opening must not be connected to an exhaust air system The pipe of the 50115191 07 2008 55 Operating Instructions HERAcell 150i 240i pressure compensation opening must not be extended or redirected 5 6 2 Installing devices without the optional gas
131. roprietary extension srf The service file is transmitted to the Technical Service of Thermo Fisher Scien tific for fault analysis I3 HERAccll 150 240 COM NoCOM 1 Create the service file gt Press the CREATE SERVICEFILE button Define file name and saving directory in the Windows dialog box 2 Start saving process gt Press OK button NOTE Duration of the procedure The compilation of device informations and the creation of the service file may take some time 50115191 07 2008 154 Operating Instructions HERAcell 150i 240 li hermo 15 Annex Data communication PASSWORD Password The submenu PASSWORD can only be accessed by the service personnel of Thermo Fisher Scientific EB HERAccll 150 240 COM NoCOM 1 GENERAL 88 6 5 66 1 DATES TIME i ERROR LOGGER DATA LOGGER SERMICEFILE PASSWORD 50115191 07 2008 155 Thermo SCIENTIFIC www Thermo com
132. s detected as an error The relay cannot be switched off see chap 5 10 pg 61 1 To toggle between two states 1 gt Press the key 4 or gt Press the key 2 2 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Options menu 50115191 07 2008 85 HERAcell 150 i 240 i 00 xxx 2008 00 00 Alarm relay Power failure on si off 4 current 2 3 Fig 51 Alarm relay setting Operating Instructions HERAcell 150i 240 7 Handling and control Setting low humidity If condensation occurs on the culture containers due to high relative humidity the humidity in the work space can be set to a lower level At the factory the device control is preset to high humidity approx 93 relative humidity By enabling the Low Humidity feature the relative humidity in the work space is lowered from approx 93 to approx 90 The modification requires an extended adaption phase To effectively prevent condensation on culture containers it must be used as a permanent setting 1 To toggle between two states 1 gt Press the key 4 or gt Press the key 2 2 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Options menu NOTE Low humidity The enabling disabling of the Low humidity function is entered into the event list If the Low humidity function is enabled the correspo
133. see chap 15 8 pg 147 Device Manager File Action View Help lB Network adapters B Ports COM amp LPT Communications Port COM1 x Communicat OM ECP Printer Port a USB Serial Port COM3 EE HHO Sound video and game controllers m 9 System devices WIE The driver can be run under the following operating systems WIN 2000 WIN XP WIN VISTA 126 Operating Instructions HERAcell 150 240 i i hermo 15 Annex Data communication 15 1 3 Installing the USB port driver Connect the USB cable optional to the control box of the HERAcell 150 i 240 i and to a PC As soon as the Windows Hardware Detector has identified the USB port the FIND NEW HARDWARE WIZARD dialog box opens Windows dialog box 1 Select the INSTALL FROM A LIST OR SPECIFIC LOCATION ADVANCED option Found New Hardware Wizard Welcome to the Found New Hardware Wizard This wizard helps you install software for EVAL232 Board USB lt gt Serial Ae If your hardware came with an installation CD or floppy disk insert it now What do you want the wizard to do automatically Recommended spectic location amp dvanced Click Next to continue 50115191 07 2008 127 Operating Instructions HERAcell 150 240i 15 Annex Data communication 2 Select the data CD as source Found New Hardware Wizard Please choose your search and installation options ey Search for the best driver
134. sent and contamination of the surroundings of the device may occur when service or repair works are carried out Within the scope of national and international legal regulations such as responsibility of a company for the protection of its employees responsibility of the operator for the operational safety of devices all possible hazards must absolutely be prevented Prior to any calibration service and repair works prior to any relocation of a device and prior to the shut down of a device the device must be decontaminated disinfected and cleaned as required by the work to be carried out Therefore we ask you to fill in this certificate of decontamination before you start with the required work Yours sincerely Thermo Electron LED GmbH nee Declaration of possible contamination please mark where applicable O The device is clear of dangerous chemical substances The device is clear of radioactivity peg The device is clear of other dangerous substances The device is clear of biological material The device is clear of cytostatic agents Certification Prior to carrying out the required work we have decontaminated disinfected and cleaned the device as described in the operating instructions of the device and in accordance with nationally applicable regulations The device does not present any hazards Date legally binding signature stamp 50115191 07 2008 125 Operating Ins
135. structions HERAcell Disinfection procedure 50115191 07 2008 Disinfection stages 150 i 240 i Thermo 9 2 Decontamimation procedures The operator must prepare hygiene regulations for the decontamination of the device in accordance with the application of the device The following disinfection procedures are compatible with the device Wipe spray disinfection The wipe spray disinfection is used as the standardized manual disinfection procedure for the device and for all accessories contra con decontamination routine The contra con decontamination routine uses an automated program cycle to decontaminate the complete work space including the shelf system and the sensors 9 3 Wipe spray disinfection The manual wipe spray disinfection is carried out in three stages Predisinfection cleaning final disinfection A CAUTION Alcoholic disinfectants form in combination with air easily combustible and explosive gas mixtures When using such disinfectants avoid open flames or exposure to excessive heat during the entire disinfection process Use such disinfectants only in adequately ventilated rooms After the disinfectant has been allowed to react wipe the cleaned device components thoroughly dry Observe safety regulations to avoid fire and or explosion hazard caused by alcohol containing disinfectants ZH 1 598 Disinfectants with an alcohol content of more than 10 may Chloride conta
136. t The input dialog allows the color contrast of the operating panel to be set within the value range of 48 to 80 1 To increase the value gt Press the key 4 2 To reduce the value gt Press the key 2 3 The value change appears in the display 1 4 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Settings Setup menu Setting the key tone The input dialog allows the loudness of the key tone that sounds whenever a key is depressed to be set The value range is 0 to 100 The change occurs in increments of 5 1 To increase the value gt Press the key 4 2 To reduce the value gt Press the key 2 3 The value change appears in the display 1 4 To accept and save the change gt Press the ENTER key 3 gt The system returns to the Settings Setup menu 50115191 07 2008 79 Menu gt Settings gt Contrast 00 xxx 2008 00 00 HERAcell 150 i 240 i Display contrast current Fig 42 Display contrast setting Menu gt Settings gt Key tones HERAcell 150 i 240 i 00 xxx 2008 00 00 Key tone volume D current 5 25 2 3 4 Fig 43 Key tone setting Operating Instructions HERAcell 150i 240 7 Handling and control Setting the interface baud rate The input dialog allows the setting of the stepping rate for data communication of the e RS232 interface e USB interface option
137. t The system returns to the Event Logger menu 50115191 07 2008 Menu gt Event logging gt Error table 1 2 HERAcell 1501 2401 00 xxx 2008 00 00 Loop Date Time Error 001 002 Sys 09 05 08 10 02 Sys 13 04 08 08 15 Error contra con Error auto start r H 10 03 08 14 20 No water r H 10 03 08 14 18 No water r H 10 03 08 14 05 No water Sys 10 03 08 13 50 Error auto start Temp 09 03 08 14 10 Nominal value high Temp 09 03 08 14 05 Nominal value high Temp 09 03 08 14 00 Nominal value high r H 09 03 08 13 45 No water r H 08 03 08 13 40 No water ex Continue 3 4 Fig 50 Error table display Operating Instructions HERAcell 150i 240 7 Handling and control Menu gt Options gt Alarm relay NOTE Troubleshooting For a detailed overview of causes for errors and their correction please refer to the end of this chapter 7 11 3 Options The input dialogs of the OPTIONS category comprise all settings of the functional device options Alarm relay Low humidity Gas tight screen Water level sensor Audible alarm Bottle turning device optional O optional Setting the alarm relay The alarm relay is the interface for the connection of the device integral monitoring system to an external monitoring system Depending on the required input signal of the external monitoring system network monitoring can be enabled or disabled If network monitoring is enabled a power failure i
138. ta communication All characters sent and received in the data exchange between a PC and the incubator HERAcell 150 i 240 are ASCII characters that can be displayed on a conventional terminal This ensures that communication can be set up controlled and programmed easily 15 2 1 Description of the protocol Character coding ASCII characters capital letters are not allowed Reading parameters Query aaaa bb cc lt CR gt or aaaa bb XXXX cc lt CR gt Response l aaaa bb XXXXX cc lt CR gt where aaaa parameter address bb amount of payload in this telegram 00 ff cc checksum Inverted XOR of all bytes without checksum and lt CR gt XXXX bb payload bytes Description of the response elements aaaa parameter address bb amount of payload in this telegram 00 ff cc checksum Inverted XOR of all bytes without checksum and lt CR gt Example of a software version query 50111927 Query 0001 00 cc lt CR gt Response 1 0001 08 50111927 cc CR Writing parameters Command aaaa bb XXXXX cc CR Response aaaa bb cc lt CR gt where aaaa parameter address bb amount of payload in this telegram 00 ff cc checksum Inverted XOR of all bytes without checksum and CR XXXX bb payload bytes Response with an error message Response I aaaa bb XX cc CR Description of the response elements aaaa parameter address bb amount of payload always 02 cc checksum Inver
139. ted XOR of all bytes without 129 Operating Instructions HERAcell 150 i 240 i Thermo checksum and lt CR gt XX 2 bytes error message see table below Example of an unknown command Query 0005 00 cc lt CR gt Response 1 0005 02 1 cc lt CR gt Significance of the two bytes in the error mesage Error in telegram structure or checksum Unknown command or unknown parameter Internal memory error Data error value not within its limits 15 3 Overview of general parameters addresses 0xxx General parameters General parameters are system values such as date time and motherboard version number 15 3 1 Reading general parameters Address Description we 9 0001 Motherboard version number Output date and time 17 byt d val 0010 hours minutes seconds ytes decimal valuen format xx Xx XX XX XX XX day month year 0011 Date 8 bytes decimal value in day month year format xx xx xx Time 8 bytes decimal value in 0012 hours minutes seconds format xx xx xx 50115191 07 2008 130 Operating Instructions HERAcell Incubator parameters 50115191 07 2008 150 i 240 i 15 4 Overview of incubator parameters addresses 2xxx 1 Incubator parameters are divided into the basic parameters of the three control loops temperature CO and 02 the internal function parameters of operating functions and data logging 5 4 1 Reading basic parameters adaress
140. ter a manual or automatic changeover of the gas supply another changeover is inhibited for 30 seconds It takes approximately 2 minutes before the filling level of the new cylinder has been determined and can be displayed The gas monitoring system monitors the filling state of the two connected gas cylinders If one cylinder is empty no audible alarm sounds but an error message is displayed an entry is written into the event list If both bottles are empty an audible alarm sounds and the alarm relay swit ches an error message is displayed an entry is written into the error table an entry is written into the event list 50115191 07 2008 91 HERAcell 150 i 240 i 00 xxx 2008 00 00 Qe 4 9 Calibration Fig 59 Gas monitoring icons Operating Instructions HERAcell 150i 240 7 Handling and control Menu gt Keypad lock NOTE Cylinder changeover A manual or automatic changeover of a gas cylinder is entered into the event display 7 11 5 Enabling disabling the keypad lock This input dialog box allows the keypad lock to be enabled or disabled At the factory the keypad lock is preset to the standard code 0000 1 Enter the 4 digit code using the keypad The input appears encrypted in the display 1 2 To delete complete incorrect input gt Press the DELETE key 2 3 To break off the input gt Press the BACK key 4 The system returns to the Configuration menu 4 To c
141. to start routine to 37 C ambient temperature 20 C Heat transfer to environment at 37 C during contra con decontamination Humidity demineralized distilled or autoclaved or completely deionized distilled or autoclaved Water quality Liquid quantity Incubation operation max 4 5 min 1 8 contra con disinfection operation 350 Constant humidity at 37 C high humidity mode approx 93 Constant humidity at 37 C low humidity mode approx 90 EMC class Sound pressure level DIN 45 635 Part 1 Relative humidity of environment Location elevation max 2000 1 determined on the basis of DIN 12880 for devices with standard equipment See detailed information in calbration instructions 50115191 07 2008 122 Operating Instructions HERAcell 150 2401 i hermo HERAcell 240 i Been Um 9 9 3 CO gas supply system Gas purity min 99 5 or medical quality Prepressure min 0 8 max 1 Measuring and control range 0 20 Control deviation temporal 0 1 CO measuring cell Accuracy absolute gt 0 3 005985 supply system Gas purity min 99 5 or medical quality Prepressure min 0 8 max 1 Measuring and control range 1 21 or 5 90 Control deviation temporal 0 2 O measuring cell Accuracy absolute 96 O5 a 2 ih a Electrical system V 1 N PE 230 V AC 10 Rated voltage V 1 N PE 120 V AC 10 V 1 N PE 100 V AC 10 Tnterterence sup
142. tructions HERAcell RS 232 configuration USB 1 1 configuration 50115191 07 2008 150 i 240 i Thermo 15 1 Interfaces 15 1 1 RS 232 interface The RS 232 data communication interface has been designed for a cable connection with 9 pin connector and a contact assignment of 1 1 Setting the transmission speed 9 600 57 600 baud 8 data bits 1 stop bit no parity Data exchange is accomplished via a defined command sequence structure see below 15 1 2 USB port optional As an alternative to data communication via RS 232 interface the devices can be equipped with the optional USB port The USB port complies with Standard USB 1 1 and is compatible with Standard USB 2 0 full speed The USB port is operated as a virtual COM port Therefore the port transmission speed can be changed within the defined baud rates 9 600 19 200 38 400 57 600 baud Data exchange is accomplished via a defined command sequence structure The command sequences comply with the design of the RS 232 interface NOTE Installing the USB port with the virtual COM port If the USB port is to be used for data exchange between PC and incubator the USB port is installed as virtual COM port USB serial port using the supplied driver The assigned COM port can be located in the Windows Device Manager Ports dialog box e g USB Serial Port COM5 and is then defined as communication port in the Heracell 150 i 8 240 i program
143. ut off valves Disconnect the gas pressure hoses from the sleeve at the rear of the device Clean the work space and wipe the device dry Until the device is shut down the work space must be continuously ventilated Leave the glass door and the outer door open and secure them in this state 99 Operating Instructions HERAcell 150i 240 9 Cleaning and disinfection 9 1 Cleaning Exterior surfaces 50115191 07 2008 Incompatible cleaners Some device components are made of plastic Solvents can dissolve plastics Strong acids or caustic solutions can cause to become brittle of the plastic For cleaning plastic components and surfaces do not use solvents that contain hydrocarbons solvents with an alcohol content of more than 10 or strong acids or caustic solutions Moisture sensitive components Do not spray cleaning agent onto the touchscreen and the control box at the rear of the device When wiping the device clean always make sure that moisture does not enter into these components Cleaning exterior surfaces 1 Remove dirt residues and depositions thoroughly using a solution of water and commercial detergent 2 Wipe the surfaces clean using a clean cloth and clear water 3 Then wipe the surfaces dry using a clean cloth Cleaning display Moisture sensitive display Do not spray or wipe the display with cleaner Clean display using a dry cloth of 100 micro fiber 100 Operating In
144. value b CO actual value 6 Key for starting the contra con decontamination routine 7 Key for opening the menu navigation 8 Key for starting the auto start routine 9 O5 display 10 O5 actual value 11 O gt set value 12 Temperature actual value 13 Temperature set value 14 Current date and time Structure of the operating levels Fig 33 Operation is divided into three levels A Direct access to the control loop settings Temperature CO O set value and bottle turing device optional B Startofthe device routines contra con or auto start C Navigation through the submenus for device configuration 50115191 07 2008 00 xxx 2008 00 00 HERAcell 150 i 240 i s Fig 31 Operating panel withoutO2 N gas supply We 150 i 240i 00 xxx 2008 00 00 1 2 3 a ae T l l 36 9 4 11 21 9 Ex SCIEN rmo Fig 32 Operating panel with combined gas connection Operating Instructions HERAcell 150i 240 hermo Showing the trend Carrying out calibration Setting value Showing the trend Carrying out calibration Setting value Settings Event logging Options Icon description Keypad lock Software versions 50115191 07 2008 Show trend Calibration Set value Show trend Calibration Set value Starting ee 3 E 9 HERAcell 150 i 240 i 00 xxx 2008
145. y are constant the automatic adjustment of the CO O measuring system is performed The CO Os control supplies the set amount of CO Os If the auto start routine has been completed the progress indicator is hidden and the main menu is displayed The device is operational Loading the device 12 Load the work space with cultures or place bottles onto the bottle turning device rollers NOTE Duration of the auto start routine When the device is cold and when the ambient temperature is low the auto start routine may take up to 10 hours Loading To ensure sufficient air circulation and even heating of the samples the loading surface within the work space should be used up to 70 max Voluminous objects in the work space that dissipate heat may impair heat distribution 64 Operating Instructions HERAcell 150i 240 7 Handling and control 7 1 Power switch Fig 29 Depending on which side the door hinges are installed the power switch 1 is integral to the front cover 2 of one of the front device stands To turn the device on Press the power switch 1 the switch illumination comes on After a short audible signal and a short dark phase the touchscreen display appears The control loop sensors pass the heat up phase see chap 7 4 pg 68 Toturn the device off Press the power switch the switch illumination goes off 7 2 Operating panel and operating structure Fig 30 Th
146. yste teret a 4 9 Supply Interface decet aoa 4 10 Work space components 44 cece ae ee eee eet trennt ennt nnns 4 10 1 Interior 181 6 54 555 cece cee 9 44 tenens 4 10 2 Optional split gas tight screens eee eeceteee ee ee eee eeee aaa 4449 44558556 5 4 10 3 Water FeSOFVOIF occ uu sep a de su ta deep Cue 8 4 10 4 Gas humidification optional only for Os 4 10 5 Heating System crore un en rase see 10 10 11 11 12 12 12 13 14 14 14 14 16 16 17 17 Operating Instructions HERAcell 150 i 240 i 50115191 07 2008 4 10 6 Rear panel openings seessssssseseseeeenerenen nennen nnne nnn 488586 46 4 10 7 Shelf System ERE Se RARE Ie 47 4 10 8 Bottle turning device for HERAcell 240 i optional suse 47 5 Start up 49 5 1 Preparing the work space nennen nnne nennen nnns 49 5 2 Installing the shelf system
147. ystem connect the gas supply lines as follows e Distributor connection 5 for the CO gas supply for up to three additional devices For looping the gas supply through interconnect the devices with a gas pressure hose Connect the gas container B to the upper connection 2 of the gas monitoring system connect the gas container A to the lower connection 3 of the gas monitoring system The outlet of the gas monitoring system 4 is connected at the factory to the gas inlet filter b by a short gas pressure hose 6 NOTE Looping the gas supply through By looping the gas supply through up to 4 devices can be supplied by a CO gas supply 5 6 4 Combined CO O N connection with gas monitoring system optional The gas supply from the gas supply system to the device is established by the supplied flexible gas pressure hoses see chap 5 6 1 pg 47 Fig 26 Devices with the combined CO2 02 N2 connection that are equipped with the optional gas monitoring system are connected according to connection diagram 13 and have the following gas connections O N gt supply e Distributor connection 1 for the O2 N5 gas supply for up to three additional devices For looping the gas supply through interconnect the devices with a gas pressure hose Connect the gas container B 2 to the upper 50115191 07 2008 57 HERAcell 2 4 B gt co EN

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