Liquid Nitrogen store:Lab 80K usage
Contents
1. The a Sheffield Biorepository Standard Operating Procedure Liquid Nitrogen store Lab 80K usage Document History Document BIO SOP 33 Number Created By Kevin Corke SUPERSEDED Version 1 Date 06 02 10 Review Date 06 02 11 Approved by Professor lan Sabroe Sheffield Teaching Hospitals NHS ie e NHS Foundation Trust S j University Cap fy css ie Sheffield Liquid Nitorgen store lab 80K usage Document Reference Page 2 of 3 1 Background This is an SOP for use of the LN2 storage tanks lab80Ks This facility contains large volumes of Liquid Nitrogen and therefore extreme care should be taken and lone working is not permitted The SOP on accessing the facility BIO SOP 30 should be adhered to first if the O2 depletion alarm is activated the facility should not be entered If at any stage whilst in the facility the alarm is activated the room should be vacated immediately The Cryogenic store user manual BIlO POLICY 24 should be used in conjunction with this SOP 2 Procedure 1 The LAB80K s operate automatically filling with Liquid nitrogen LN2 as and when required The bulk storage tank is checked on a weekly basis by the BOC cryogenic gas delivery service 2 The freezer record should be updated when using the tank recording why and when the tank was used 3 Before opening the tank put on the appropriate PPE Gloves Face Mask apron 4 Open the tank lid and then press demist L2. eave a couple of minutes until the storage racks can be seen 5 Turn the racks until the required rack can be seen the rack can then be lifted Care should be taken at this stage as the full racks will be heavy and therefore care should be taken 6 Pull out the required storage box and place remove the required Samples 7 Return the storage box to the rack and then return the rack to the LAB80K NOTE NEVER REMOVE MORE THAN ONE RACK FORM THE TANK AT A TIME TO AVOID MISTAKES WHEN RETURNING THE RACKS TO THE LAB80OK 8 Close the lib and on the controller press auto fill to replenish the LN2 level 3 Quality Assurance Control The audible visible and remote alarms on the tank should be tested on a weekly basis and recorded on the freezer record To test the alarm On the control panel select Menu 3 4 and follow the on screen instructions until you can hear a audible chirping and activation of the visible and remote alarms Data Logs all events involving the Lab80Ks will be recorded on the Sheffield Teaching Hospitals NHS NHS Foundation Trust The University i 7 fy Of us Sheffield Liquid Nitorgen store lab 80K usage Document Reference Page 3 of 3 data log which can be accessed via the connected PC 1 Open up the programme on the laptops LN2 controller M305 505 2 Click Identify M305 505 start scan The system will then make connection to the Lab80K tanks in operation If found the screen
3. will indicate Identified 3 Go to status and view data log The data log should then be saved to a pre prepared location on the University network Save the file using the default name avoids easing of saved records These can then be viewed archived 4 Associated Documents Document O Document Reference Freezer Store access record BIO FORM 01 2 SOP In the event of O2 depletion alarm BIO SOP 08 LN2 store Risk assessment BIO RA LN2store a LN2 store security and Access BIO SOP 30 5 Cryogenic store user manual BIO POLICY 24 6 Freezer record BIO FORM 02 D a Sheffield Teaching Hospitals NHS NHS Foundation Trust wees The pal University SF f Sheffield
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