"user manual"
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1. volume 2 5ml 80 2 109 80 Outputs USB SD card option Glass 8 matched cells with lid 80 2109 81 Dimensions W x D x H 260 x 390 x 100mm Quartz 8 matched micro with lid min volume 400yl 80 2109 82 Weight lt 4 5 kg SPECTROPHOTOMETERS LIGHTWAVE II BIOWAVE II ae Stored methods 90 All products are CE marked and comply with relevant legislation i ae Wavelength accuracy es ANM including EMC and low voltage directives _ Photometric reproducibility 0 002A at 0 0 5A 546nm Biowave DNA Biowave II and Lightwave products have a two year warranty Photometric accuracy 0 003A at 0 0 5A and a warranty on lamp life of three years Sane All other WPA instruments have a one year warranty Outputs Ue uee Colello 212 arapo Dimensions W x D x H 260 x 390 x 100mm As part of our policy of continuous instrument development Weight lt 4 5 kg we reserve the right to alter specifications without notice lt LLI D WY 2 O i M O Ai O 00 UV Visible Spectrophotometry UV Visible Spectrophotometry is a fundamental analytical technique and together with suitable sample handling accessories is used in laboratories for absorbance and transmission measurements of samples in all application areas Biochrom using it s Novaspec Ultrospec GeneQuant Libra and WPA brand names manufactures an extensive range of attractive UV Visible products and accessories with performance and reliability guaranteed by over 35 years experience in the fi2. UNIQUE INTEGRAL CUVETTE TRAY FOR SECURELY HOLDING EXPENSIVE CELLS for long lamp lifetimes together with simple selection software and large graphical display for ease of use and data interpretation The stored methods include DNA RNA and oligonucleotide calculations protein assays such as direct UV measurement BCA Biuret Bradford and Lowry and cell density measurement Unlike many dedicated life science instruments the Biowave DNA can also 80 3004 70 80 3004 71 80 3003 84 80 3004 07 80 3004 73 80 3005 10 measure Absorbance or concentration at any wavelength so there is complete flexibility for future applications For added convenience it is possible to display a scan of the nucleic acid profile which is particularly useful for RNA samples where impurities may be present in the 230 nm region yet not have an adverse effect on the 260 280 Absorbance ratio The system is compatible with both Quartz and disposable low volume UV cuvettes Results may be printed to an optional integrated high quality graphical printer for permanent record or exported via USB cable connection or SD Card to a suitable PC running optional Print Via Computer PVC software for advanced reporting or data storage The Lightwave Il diode array UV Visible spectrophotometer is the perfect combination of ease of use with flexibility incorporating Novel Optics with no moving parts and a Xenon source for high energy performance wi
3. ON WIRELESS BLUETOOTH CONNECTIVITY OPTION SD CARD FOR DATA STORAGE amp EXPORT OPTION UNIQUE INTEGRAL CUVETTE TRAY FOR STORAGE OF EXPENSIVE CELLS AND SUPPORT OF VALUABLE SAMPLES There are pre defined methodologies for nucleic acid quantification DNA RNA and The Biowave Il diode array spectro photometer the benefits described for the Lightwave Il with the offers all oligionucleotides protein assays BCA addition of key life science applications Biuret Bradford and Lowry and for cell ORDERING INFORMATION Biowave II UV Visible Life Science Spectrophotometer 80 3003 75 Biowave II UV Visible Life Science Spectrophotometer with printer 80 3003 76 Biowave II UV Visible Life Science Spectrophotometer with Bluetooth 80 3003 77 Biowave II UV Visible Life Science Spectrophotometer with SD card 80 3005 11 Biowave ll UV Visible Life Science Spectrophotometer 80 3004 80 80 3004 81 Biowave ll UV Visible Life Science Spectrophotometer with printer Biowave ll UV Visible Life Science Spectrophotometer with Bluetooth 80 3004 82 80 3005 12 80 3003 84 80 3004 07 80 3003 96 80 3005 00 Biowave ll UV Visible Life Science Spectrophotometer with SD card Printer accessory Spare printer paper 20 rolls Bluetooth accessory SD card accessory with PVC software A WORKHORSE FOR THE MOLECULAR BIOLOGIST culture density measurements The visualision of the nucleic acid scan is particularly useful espe
4. OPTICS FOR HIGH ENERGY COMBINED WITH A XENON SOURCE FOR LONG LAMP LIFETIME M UNIQUE INTEGRAL CUVETTE TRAY FOR STORAGE AND SAMPLE SUPPORT E WAVELENGTH SCANNING KINETICS AND CONCENTRATION FUNCTIONALITY WITH FULL GRAPHICS DISPLAY WE INTEGRATED PRINTER OPTION M WIRELESS BLUETOOTH CONNECTIVITY OPTION M INTEGRATED SD CARD ACCESSORY FOR DATA STORAGE amp EXPORT OPTION E SIMPLE SELECTION SOFTWARE ORDERING INFORMATION Lightwave II UV Visible Spectrophotometer Lightwave II UV Visible Spectrophotometer with printer Lightwave II UV Visible Spectrophotometer with Bluetooth Lightwave II UV Visible Spectrophotometer with SD card Lightwave II UV Visible Lightwave II UV Visible Lightwave II UV Visible Lightwave II UV Visible Spectrophotometer Spectrophotometer with printer Spectrophotometer with Bluetooth Spectrophotometer with SD card 80 3003 72 80 3003 73 80 3003 74 80 3005 13 80 3004 60 80 3004 61 80 3004 62 80 3005 14 LIFE SCIENCE ORIENTED PRODUCT WITH STORED ROUTINES FOR NUCLEIC ACID QUANTIFICATION PROTEINS CELL DENSITY Biowave Il Life Science Spectroohotometer NOVEL GIFFORD OPTICS FOR HIGH ENERGY COMBINED WITH A XENON SOURCE FOR LONG LAMP LIFETIME SIMPLE SELECTION SOFTWARE WITH STORED METHODS FOR LIFE SCIENCE APPLICATIONS WAVELENGTH SCANNING KINETICS AND CONCENTRATION FUNCTIONALITY WITH FULL GRAPHICS DISPLAY NUCLEIC ACID SCANS FOR PURITY CHECKING INTEGRATED PRINTER OPTI
5. OURCE EASY TO USE EASY TO CLEAN EASY TO STERILIZE RECHARGEABLE BATTERY THAT WILL LAST UP TO ONE MONTH ORDERING INFORMATION CO 8000 Personal Cell Density Meter mains rechargeable battery 80 3000 45 OPTIMISED FOR THE TEACHING LABORATORY S800 Spectrawave Visible Diode Array Spectrophotometer M ABSORBANCE TRANSMISSION CONCENTRATION AND KINETICS E LARGE EASY TO READ DISPLAY E GRAFICO PC UTILITY SOFTWARE M EDUCATIONAL EXPERIMENTS AND UV VISIBLE TUTORIAL E ANALOGUE OUTPUT FOR CONNECTION TO CHART RECORDER The S800 Visible spectrophotometer has been designed to meet the needs of both students and technical staff in education The instrument is small and light in weight for portability with a large display for ease of reading The S800 measures Absorbance Transmission and Concentration as well as being able to output absorbance time plots directly to chart recorder In addition the user manual includes simple experiments for the determination of A max extinction coefficient and natural bandwidth as well as the construction of a standard curve and the measurement of stray light The instrument is delivered with the Grafico PC utility software package and serial lead providing ORDERING INFORMATION 800 Visible Spectrophotometer the student with the means to capture print and interpret all results including a wavelength scan on a PC Data may be easily exported from Grafico into E
6. WPA Biochrom Ltd and UV Visible Soectroohotometry WPA Ltd was acquired by Biochrom Ltd in 2002 because their range of colorimeters and innovative spectrophotometers provided a perfect extension for the Ultrospec and Libra range of UV Visible spectrophotometers Together these brands address the market from hand held colorimeters up to Pharmacopoeia compliant high resolution products The products are now designed and manufactured at the Biochrom factory on the famous Cambridge Science Park in the UK UV Visible Spectrophotometry is a popular analytical technique used in most laboratories for a whole host of applications and across the Biochrom group there are products for every occasion The guide below will assist with your WPA brand selection WPA BRAND SELECTION GUIDE PRODUCT Colorimeters LIGHT SOURCES OPTICAL SYSTEM INSTRUMENT PARAMETERS COMMENT CO 7000 Tungsten Filters 400 440 470 0 3 to 1 99A 40nm lt 1 T at filter Tropicalised colorimeter 4907520550 wavelength Ideal for use in hot 530 590 630 humid remote locations 700nm for clinical medical applications CO 7500 Tungsten Filters 440 470 490 0 3 to 1 99A 40nm lt 1 T at filter Robust colorimeter that 520 550 580 wavelength is ideal for schools 590 680nm and colleges CO 8000 600nm LED LED 600nm 0 3 to 1 99A 40nm lt 1 T at Cell density meter for E Coli 600nm and Yeast cell culture OD600 measurements Spectrophotometers S800 Tungsten Single b
7. ad providing the user with the means to capture print and interpret all results so that a results log may be built up on a PC Data may also be easily exported from Grafico to Excel Graphics may be printed to the industry standard Seiko DPU 414 printer for a permanent record and kinetics data may be output to chart recorder The 1200 accepts standard 10mm pathlength glass or plastic cuvettes or 10 12 16mm test tubes with the optional adapter enabling COD measurements to S1200 Spectrawave Visible Diode Array Spectrophotometer VISIBLY FASTER be made using standard 16mm diameter test tubes plus the cell holder may be removed for cleaning Another version of the instrument the WPA S1200T is available with a factory fitted electrically heated cell holder for thermostatted measurements at 37 C The S1200 is a versatile Visible spectrophotometer for all laboratories WITH FULL GRAPHICS M CHART RECORDER OUTPUT SIMPLE MENU DRIVEN SOFTWARE E GRAFICO PC UTILITY SOFTWARE M WAVELENGTH SCAN KINETICS AND STANDARD CURVE FUNCTIONALITY M COMPREHENSIVE 99 METHOD STORAGE ORDERING INFORMATION 1200 Visible Spectrophotometer 80 3003 58 1200T Visible Spectrophotometer with heated cell holder 80 3003 59 Test tube adapters 10 12 16mm 80 2117 47 Sp
8. are lamp 80 2115 33 Chart recorder interface cable _ 80 3003 55 Seiko DPU 414 printer 80 2108 80 Serial cable for Seiko DPU 414 printer 80 2118 18 Biowave DNA FULL GRAPHICS DISPLAY AND VALUABLE SAMPLES The Biowave DNA has been specifically designed for life science applications and is a powerful tool for the laboratory that requires a dedicated instrument for the determination of nucleic acid purity and concentration protein concentrations or cell density measurements The system utilises Novel Optics for high energy throughput a Xenon light source ORDERING INFORMATION Biowave DNA UV Visible Life Science Spectrophotometer Biowave DNA UV Visible Life Science Spectrophotometer with printer Printer accessory Spare printer paper 20 rolls Print via computer software and cable Biowave DNA UV Visible Life Science Spectrophotometer with SD card Life Science Spectroohotometer NOVEL OPTICS FOR HIGH ENERGY COMBINED WITH A XENON SOURCE FOR LONG LAMP LIFETIME SIMPLE SELECTION SOFTWARE WITH STORED METHODS FOR LIFE SCIENCE APPLICATIONS A WORKHORSE FOR THE MOLECULAR BIOLOGIST NUCLEIC ACID SCANS FOR PURITY CHECKING INTEGRATED PRINTER OPTION COMPACT SPACE SAVING DESIGN COMPATIBLE WITH LOW VOLUME CUVETTES INTEGRATED SD CARD ACCESSORY FOR DATA STORAGE amp EXPORT OPTION
9. cially for RNA samples where impurities may be present in the 230 nm region yet not have an adverse effect on the A260 A280 ratio The system is compatible with disposable low volume UV cuvettes The combination of the life science methods with the rapid scanning kinetics and concentration capabilities of the Biowave II make it a very useful addition to any molecular biology laboratory In kinetics mode the basic plot of absorbance against time may be supplemented with the result for 6A min plus the correlation coefficient is also calculated for the duration of the assay This slope may be multiplied automatically by a factor to convert it directly to rate of reaction Once again all results may be printed to an optional integrated high quality printer for permanent record or the instrument may be linked to a PC via a USB cable connection optional wireless Bluetooth or SD Card accessories for data storage or printing Cells all 10mm pathlength Technical Specifications Light source optical system wavelength range absorbance range O rd r N o G Uu q bandwidth and stray light at 340nm are shown at the front of this brochure Other parameters are shown below PARAMETER COLORIMETERS DESCRIPTION PART NUMBER CO7000 CO7500 CO7500B CO8000 Stored methods n a D ispOsa b e cel Is Wavelength accuracy n a Acrylic pack of 100 volum 2 5m 80 2004 53 He L reproducibility E e a Polystyrene pack of 100
10. e in small research labs where cultures may be grown in 200ml to 5 litre volume conical flasks the CO 8000 may be taken to the area of the lab where the cells are grown or used in incubation cabinets or under anaerobic conditions Up to 99 results may be stored for subsequent recall printing or download to spreadsheet Since it can accept either 10mm pathlength cuvettes or tubes the instrument may be used with Ehrlenmyer side arm flasks In addition cell culture spillages can be easily wiped from the smooth surface and then removed from the cell compartment area by pouring ethanol through the unit Sterilization may be achieved by pouring through formaldehyde or ethylene oxide The instrument has rechargeable batteries CO 8000 Biowave Personal Cell Density Meter RAPID LOW COST CELL DENSITY MEASUREMENT ANYTIME ANYWHERE that are automatically charged when it is connected to the mains This allows almost 1 month use under normal operating conditions when fully charged offering great flexibility and portability A 600nm LED source in combination with a fibre optic is used to obtain the optical density measurement The instrument may be linked via a serial lead to either a serial printer for hardcopy output or to a PC for download of results to spreadsheet SMALL PORTABLE AND DEDICATED CELL DENSITY METER THAT MAY BE USED WHERE CELLS ARE ACTUALLY CULTURED MEASURES AT 600nm USING A LONG LIFETIME LED S
11. eam 330 800nm 0 3 to 2 5A 7nm lt 1 T at 340nm Scanning visible instrument Monochromator for education 1200 Tungsten Single beam 330 800nm 0 3 to 2 5A 7nm lt 1 T at 340nm Scanning visible instrument Monochromator for QC and routine use Biowave DNA Xenon Dual channel 190 1100nm 0 3 to 2 5A 5nm 0 5 T at 220 Dedicated life science Monochromator and 340nm product with stored routines for nucleic acid protein and cell density measurements Lightwave II Xenon Dual channel 190 1100nm 0 3 to 2 5A 5nm 0 5 at 220 Scanning instrument for Monochromator 3nm versions and 340nm general UV Vis applications Biowave II Xenon Dual channel 190 1100nm 0 3 to 2 5A 5nm OS 7ol at 220 Life Science oriented product Monochromator 3nm versions and 340nm with stored routines Other instrument specification parameters are shown on page 11 for nucleic acid protein and cell density measurements with software for general uv vis applications The CO 7000 is a portable colorimeter designed for use by doctors and medical technologists in small and medium sized clinics The unit has been tropicalised to protect it in hot and humid conditions to 45 C and 70 respectively The 10 gelatin filters are encased in glass to prevent fungal growths appearing and the PCB has been conformally coated so that individual components are sealed to prevent corrosion The instrument is powered by an internal rechargeable NiMH battery or by external p
12. eld Amongst other technological advances these instruments feature PTR Press to Read capability which dramatically extends the lifetime of the source lamps Microtitre Plate Readers Washers Dispensers and Luminometers In the food testing clinical biotech and pharmaceutical industries the demand is for ever increasing sample throughput and smaller and smaller volumes This is where the microtitre plate comes into its own and Biochrom offer an excellent range of fast versatile and reliable plate readers with user friendly designs via its Asys and Anthos product lines In addition a range of microplate washers is available with a unique manifold design for minimised residual volumes and digitally controlled aspiration and dispensing pumps for high accuracy and low noise performance Amino Acid Analysis Biochrom has been in the field of dedicated Amino Acid Analysis for over 30 years using established ion exchange chromatography to provide rapid specific amino acid analysis for Clinical Pharmaceutical proteomics food and feedstuff industries These state of the art bench top products feature proven Ninhydrin detection technology fully integrated into a complete package utilising the latest graphical software active components in ceramic and PEEK for long life and elimination of contamination and a range of robust ion exchange columns for customised applications
13. ludes test tube adapter set mains rechargeable battery 80 3000 42 Spare lamp CO 7000L 80 3000 55 Spare filter set CO 7000F 80 3000 56 Recommended methods for these routine clinical chemistry assays together with full details of reagents required manual colorimetric procedures calibrations and quality assurance may be found in District Laboratory Practice in Tropical Countries Parts 1 amp 2 2nd edition by Monica Cheesbrough from Cambridge University Press or other similar publications SMALL INEXPENSIVE COLORIMETER FOR THE TEACHING ENVIRONMENT CO 7500 Colourwave Educational Colorimeter E DESIGNED WITH THE STUDENT USER IN MIND E RUGGED PORTABLE AND EASY TO USE WE EXTREMELY VERSATILE WE RECHARGEABLE BATTERY VERSION AVAILABLE The CO 7500 is a value for money instrument that has been designed for use in educational establishments including sixth form colleges secondary schools technical schools and colleges With a large clear digital display and simple push button controls the instrument is ideal for students The unit is compact and robust enough to withstand the rigours of the teaching environment and is available in mains only or mains internal rechargeable NiMH battery versions The CO 7500 is easy to use The eight filters at 440 470 490 520 550 580 ORDERING INFORMATION CO 7500 Educational Colorimeter mains only CO 7500B Educational colorimeter mains rechargeable battery Spare lam
14. ower allowing it to be used where the power supply could be unreliable The CO 7000 is very easy to use as there are only three buttons and the wavelength required is selected by rotating an integral filter wheel The filters at 400 440 470 490 520 550 580 590 680 and 700nm enable assays in the wavelength range 400 to 700 nm to be measured and the instrument has been designed as an open system so that test kits for clinical and medical applications from virtually any supplier may be used Examples of CO 7000 Colourwave Medical Colorimeter PORTABLE INSTRUMENTS FOR THE SMALL MEDICAL CLINIC routine assays that may be measured in serum and plasma include Albumin Cholesterol Glucose Creatinine Total Protein Urea and those in cerebrospinal fluid include Glucose and Total Protein The samples may be measured in either standard 10mm pathlength cuvettes a minimum of 400ul is required or in 10 12 16mm_ diameter test tubes adapters are included with the instrument There is a drain hole at the bottom of the cell compartment so that spillages do not affect the instrument E FULLY TROPICALISED AND PORTABLE M READS ASSAYS IN THE WAVELENGTH RANGE 400 TO 700 nm USING MANY PROPRIETARY TEST KITS E EASY THREE BUTTON OPERATION ON OFF REFERENCE AND TEST RECHARGEABLE BATTERY M REGISTERED FOR IVD APPLICATIONS ORDERING INFORMATION CO 7000 Medical Colorimeter inc
15. p CO 7500L Spare filter set CO 7500F Test tube adapter set Serial lead S2000P printer including lead 590 and 680nm are encased in an integral filter wheel and the wavelength required is selected by rotating this until the relevant colour coded number is visible in the indicator window The ergonomic design makes this very convenient and filters cannot be accidentally lost or damaged With only five buttons on off reference and test convert between Absorbance and Transmission readings and kinetics the instrument is ideal for beginners When used in kinetics mode to study rates of reaction the CO 7500 takes readings every second and these may be sent to a chart recorder via the analogue output or 80 3000 43 80 3000 44 80 3000 59 80 3000 58 80 3000 57 80 3001 00 80 3000 94 results may also be downloaded directly to a PC or data logging system The samples may be measured in either standard 10mm pathlength cuvettes a minimum of 400ul is required or in 16mm diameter test tubes adapters for 10 12mm test tubes are an optional accessory with the CO 7500 There is a drain hole at the bottom of the cell compartment so that spillages do not affect the instrument The CO 8000 cell density meter is a small portable and easy to use instrument for measuring the density of E coli and yeast cells in suspension at 600nm and has been designed to give comparable readings to other spectrophotometers Ideal for us
16. th longer lamp lifetime The instrument includes a large wide view display and in built software providing flash scan fixed wavelength measurements kinetics and concentration with comprehensive graphics capability plus the ability to store up to 90 methods On peak confirmation is also a feature of the flexible software Concentration may be measured using either a factor single point calibration multi standard curves or Lightwave II UV Visible Diode Array Soectroohotometer alternatively there is a multi wavelength mode where equations using absorbance values may be used for ratio calculations Samples may be measured in 10 20 or 40mm pathlength cells glass quartz or disposable and all results may be printed to an optional integrated high quality printer for permanent record Alternatively the instrument may be linked to a PC via a USB cable connection the optional wireless Bluetooth or SD Card accessories for data storage or printing The Lightwave Il has been designed to meet the needs of customers in most laboratory situations and is compact lightweight convenient and excellent value for money compared to conventional systems With its elegant new user interface Gifford Optics and Bluetooth connectivity the Lightwave II is an obvious choice in a multi function environment A higher resolution Lightwave Il with a 3nm bandwidth is also available SHAPING THE FUTURE OF SPECTROPHOTOMETRY M NOVEL
17. volume 1 5ml 80 2084 11 omes akadai L enn SSIS CORDS S a i UV plastic semi micro pack of 100 min volume 750ul 80 3000 77 GPN E 0 2V for 0 2A 0 1 99V UV Plastic ultra micro pack of 100 fill volume 80pl 80 3000 81 for 0 199 T CO7500 7500B Dimensions W x D x H 150 x 180 x 60 mm Glass cells Weight 0 6 kg Standard rectangular with lid volume 2 5ml 80 2003 87 Grade TET r z SPECTROPHOTOMETERS Semi micro with lid min volume 750ul 80 2004 15 S800 1200 Stored methods 99 S1200 Quartz cel Is Wavelength accuracy z 2 Standard rectangular with lid volume 2 5ml 80 2002 58 Photometric reproducibility 0 002A at 0 0 5A 546nm Semi micro with lid min volume 750ypl 80 2002 77 Photometric accuracy 0 003A at 0 0 5A Micro with lid min volume 400ul 80 2002 95 Outputs RS232C Analogue 0 2V Ultra micro fill volume 70pl 80 2103 69 Dimensions W x D x H 215 x 270 x 120mm Ultra micro fill volume 15pl 80 3000 83 Weight lt 2 kg SPECTROPHOTOMETERS Matched cells OAC Ga Glass 8 matched standard rectangular with lid volume 2 5ml 80 2109 83 Stored methods 9 Quartz 2 matched standard rectangular with lid volume 2 5ml 80 2099 89 Wavelength accuracy 2nm Quartz 2 matched semi micro with lid min volume 750ul 80 2100 13 Photometric reproducibility 0 002A at 0 0 5A 546nm Quartz 2 matched micro with lid min volume 400ul 80 2100 25 Photometric accuracy 0 003A at 0 0 5A Quartz 8 matched standard rectangular with lid
18. xcel plus Grafico also includes an educational tutorial on UV Visible spectrophotometry The S800 accepts standard 10mm pathlength glass or plastic cuvettes alternatively a test tube adapter set is available for 10 12 and 16mm tubes In case of spillage the cell holder may be removed for cleaning For completeness the instrument is delivered with a starter pack of disposable plastic cuvettes With simplicity of operation and the inclusive student package the S800 is the ideal tool for education 80 3003 50 Test tube adapters 10 12 16mm 80 2117 47 Spare lamp 80 2115 33 Chart recorder interface cable 80 3003 55 The S1200 Spectrawave Visible diode array spectrophotometer has been designed to meet the routine spectroscopy needs of customers requiring a small light weight instrument that is easy to use The benefits of diode array technology mean that as there are no moving parts the product is very reliable and requires low maintenance Compared to equivalent units on the market the S1200 offers so much more ideal for use in educational biotech or industrial establishments the 1200 measures Absorbance Transmission Absorbance ratio and Concentration The large backlit graphical display enables wavelength scans kinetic assays including slope calculation and standard curves to be viewed The instrument is delivered with the Grafico PC utility software package and serial le
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