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193_0010S_02

1. Prog ml min 0 1MPa offO ont 12 Prog P01 01 00 0200 010000710 Setup A ink lt OFF 000 0 100 gt GLP Hold 0 Time Run 1 min Fig 8 Events terminal strip Relation between Event terminal strip B and status of the individual Event contacts displayed in the LCD screen A 10 User manual LC 110S pump version 2 Entering control signals in the program 1 Select the program menu 2 Select the program number 3 Press horizontal arrow key to go to the editing mode of the sub menu 4 Enter values for events in the upper status bar by using the verti cal arrow keys Value Explanations 0 Off 1 On JL Pulse No status change Wiring of event amp remote terminal strip To enable signal transmission from external devices to the pump the flat ribbon cable is connected with a connector strip and connected to the Re mote terminal strip on the rear of the pump E Fig 9 Connection the flat ribbon cable with the connector strip 1 Place the connector strip C p n 193 0400S on a suitable surface Insert the stylus A into the opening on the top of the connector strip and press downward 3 Keep the stylus pressed down and insert the lead B of the flat rib bon cable p n 193 0402 into the front of the connector strip Description of instrument 11 4 Remove the stylus 5 Check whether the cables are secured properly Flat ribbon cables that are connected to the Events and Remo
2. Link menu Program links are combinations of existing programs and can be created and edited like any other program The Link menu displays the status of active links Max nine program links between defined programs can be created and saved A diamond icon is displayed on the left of the lower line in the display Navigating through the Link menu e Use the horizontal arrow keys to navigate through the two lines of the display e Use the number keys to enter a value Alternatively With the ver tical arrow keys you can increase and decrease the displayed val ues e Move the cursor onto the diamond icon to navigate through the sub items of the Link menu by means of the vertical arrow keys e Press START STOP or hold horizontal arrow key for approx 1 second to return to the main menu Creating a link Procedure e Select the Link menu e Enter the link number e Press horizontal arrow key to go to the editing mode of the sub menu e Move the cursor to position P in the display and enter the e program number e Move the cursor to position R R Repeat and enter the value number of repetitions for the specified program e Move the cursor to position W in the display and enter 1 or 0 Note If the W1 W Wait setting is used the pump waits for an external start signal or for the user to press number 1 before executing this line If the WO setting is used the link sequence is run without interruption 44 User
3. pindex 125041 0 MPah GLP P cycles 108408 total time DOE 8h instal 01 07 2009 service 18 01 2010 GLP H S N Head no SN head type 10ml GLP H volume ODD 8l pindex MPah GLP H cycies DODDDO total time OO000 0h GLF display DD0 51 TO0 43 Fig 18 Structure of the GLP menu 23 User manual LC 110S pump version 2 Purging the pump Purging the pump in the LPG system The purging process of the pump is limited to a max pressure of 5 MPa If this value is exceeded during the purging process the pump switches off automatically e Inthe Setup menu of the pump select Gradient Mode LPG e n purging mode select channel MIX or gradients A B C and D Note Before the purging process open the purge valve to prevent pres sure surge and damage to the column Procedure Open the bleed screw Press PURGE Select the purging mode Enter the value for the flow rate Press START STOP to start the purging process Press START STOP to stop the purging process oogAomc Load Fiza Pree Deni Prog fom Gwe oft aL Proga PURGE 3 000 000 H5MPaj eSetup Link 4 OFF channel MIX i GLP bald Tire e EE fC WD Fun 1 mia i Fiza Prego Dart Prog Pile OPa oF mig Proga PURGE 3 000 000 5MPa msetup Link zPURGE channel MIX ie GLP bald Tire uA WE fog WD Run 1 Iria Fig 19 Purging the pump in the LPG system Purging the pump in HPG or isocratic sys
4. Gradient mode Calibration option of the analog input of the Remote terminal strip for remote flow rate control At set zero an input voltage that is present can be defined as zero point i e defined as a flow of 0 00 ml min by means of the vertical arrow keys If a deviating positive control voltage is applied a resulting flow rate is dis played at actual based on the basic setting 1 ml corresponds to 1 V for the 10 ml pump head and 5 ml corresponds 1 V for the 50 ml pump head This flow rate can then be changed by press ing the vertical arrow keys Thus a second calibration point is generated which modifies the scaling Selection of a time constant to be used for signal smoothing The larger the value of the selected time constant the stronger the ap plied signal smoothing Both an external sig nal present at the analog input in and a sig nal generated at the analog output out can be smoothed The E 1 to E 8 connections of the Events terminal strip can be activated 1 or deacti vated 0 by means of the vertical arrow keys Here the brightness and contrast of the dis play can be set 21 The LC 1108 is capable of isocratic none high pressure gradient HPG and low pressure gradient LPG operation User manual LC 110S pump version 2 Setting up the gradient mode e Select GRADIENT MODE from the SETUP Menu e Select the desired mode of operation none HPG LPG e f you select either
5. Antec Industrieweg 12 2382 NV Zoeterwoude The Netherlands LC 110S pump User manual 193 00108 Edition 2 2012 Q iso 4 9001 T431715813333 F 31715813334 info myantec com www myantec com IVI certified Copyright 2011 Antec The Netherlands Contents of this publication may not be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from the copyright of the owner The information contained in this document is subject to change without notice ROXY ALEXYS DECADE DECADE II INTRO Flexcell ISAAC HyREF are trademarks of Antec Whatman word and device and Whatrnan word only are trademarks of Whatman International Ltd SOLVENT IFD and AQUEOUS IFD are trademarks of Arbor Technologies Inc Clarity DataApex are trademarks of DataApex Ltd Microsoft and Windows are trademarks of Microsoft Corporation Excel is a registered trademark of the Microsoft Corporation The software and the information provided herein is believed to be reliable Antec shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of software or this manual All use of the software shall be entirely at the user s own risk DECLARATION OF CONFORMITY The manufacturer hereby declares that the product LC 110S pump
6. 2 Enter program number 0 3 Press horizontal arrow key to go to the editing mode of the sub menu 4 Enter number of the program to be deleted and press horizontal arrow key to confirm the entry 5 Press vertical arrow key to confirm the query Wake Up Program The LC 1108 can execute a program at a preset time WAKE UP pro gram This program is preset as number 20 Make sure that the date and time of the LC 1108 are set up properly in the SET UP menu The pump starts the selected program at the specified time and the display switches to RUN mode program is running Load Pov Peeeure Gverts Prog mmn IMPs cTO oo tL Prog Open P20 01 pSetup Link a b GLP Hodo Tira A w wc wo Rant re t how Peesure Grecia Prog mmn PIMPS cTO oo tL Prog P20 Time 12 08 WAKE UP pSetup Link Date 19 01 10 P GLP Hold Tere A w Wc OND Rar re t Peesure Deci Prog reer PUMPS cTO 01012 Prog P20 Time 15 00 WAKE UP Setup Link Date 19 01 10 P01 GLP Hold Terre A we c wb Rant re Fig 34 Creating a Wake Up program Procedure 1 Select the Program menu 2 Enter program number 20 3 Press horizontal arrow key to go to the editing mode of the sub menu 4 Enter the date and time for the program start 5 Enter the number of the program that should start at the 6 preset time LC 110S programs 43 7 To interrupt the current WAKE UP program enter another 8 program number
7. A inlet fitting of the pressure sensor B Connections for piston backflush C Fixing screws of the pump head D Inlet fitting the pump head and E Outlet fitting of the pump head Installing the pump head Procedure 1 Check whether the pump head has been assembled correctly 2 Using an Allen wrench tighten the opposite pairs of fastening Screws evenly and alternately to prevent the pump pistons on the inside from jamming 3 Tighten all fastening screws evenly 4 Connect the capillary between the pump head and pressure sen Sor and tighten the screws at the pump head inlet and at the pres sure sensor inlet Exchanging the check valves A check valve is a mechanical device a valve which normally allows fluid to flow through it in only one direction The device is equipped with two check valves one at the low pressure inlet of the pump head and one at the outlet of the primary pump head chamber When a check valve is dirty it may cause incorrect opening of closure leading to pressure fluctuations and an irregular flow Removing the check valve Note Alternately loosen the screw fittings of the capillary connections to prevent the capillary from bending User manual LC 110S pump version 2 Procedure With a wrench unscrew the inlet fitting Remove the ball valve With a wrench unscrew the capillary connection Remove the outlet fitting Remove the ball valve aPN l Fig 39
8. RUN 000 16 73 17 P GLP Howo Tie A w w wp Rant re load Now Peesure Gverts Prog mmn j2 MPa cTO ot JL Prog 4 P02 1 000 000 Setup Link END 000 50 10 90 i GLP Mod D Tie A w uc wp Rant Fig 36 Running a link Deleting a link Procedure Select the Link menu Enter link number 0 Press horizontal arrow key to go to the editing mode of the submenu Enter number of the link to be deleted and press horizontal arrow key to confirm the entry 6 Press vertical arrow key to confirm the query akwWNnN gt 46 User manual LC 110S pump version 2 Load Fia Femssura Dyer Fei nin 5 5MWER ad o iLi Prag Open LO 1 02 03 msetup Link 4 F GLP Hod D Tire TA 80 BO SO Amn 1 t Fe Femamura Lveria Pec Ukin paj ari on 1 ILE Prag 4 Delete program L Setup Lirik b GLP Hind D Time uA WE no D fun prin t Fe Femamura Lveria Bscz inn Me cF on ILE Prag 4 Delete program LO petup Link confirm with cursor key F GLP Hold D Time Au WE I WD Aun 1 rin Fig 37 Deleting a link Maintenance CHAPTER 6 Maintenance 47 All moving parts are subject to normal wear and tear It is therefore un avoidable that certain parts have to be replaced once in a while The LC 1108 is constructed in a way that worn out parts can easily be reached and replaced with a minimum of downtime Proper maintenance of your HPLC device will ensure successful analyses and reproducible results
9. of parts or all of the equipment Do not proceed beyond a warning sign until the indicated conditions are fully under stood and met The attention sign signals relevant information Read this information as it might be helpful Safety practices A The following safety practices protective measures are intended to ensure safe operation of the instrument e You are only permitted to perform the maintenance tasks de scribed in this manual e All other maintenance tasks are to be performed exclusively by the manufacturer or a company authorized by the manufacturer Electrical hazards Without exception the following applies to all maintenance tasks that can be performed by the user e Switch off the device and pull the power plug e Never open a device Removal of protective panels on the instru ment can result in exposure to potentially dangerous voltages which may lead to severe injury or loss of life The instrument may only be opened by authorized service engineers of the manu facturer or a company authorized by the manufacturer WARNING RISK OF ELECTRIC CHOCK A DISCONNECT POWER BEFORE SERVICING AVERTISSEMENT RISQUE DE CHOC ELECTRIQUE COUPER L ALIMENTATION AVANT LA MAINTENANCE Place the LC 110S on a flat and smooth surface or inside a PR 110 pump rack Connect the detector to a grounded AC power source line voltage 100 240 VAC frequency 50 60 Hz The instrument should be connected to a
10. 110S behind the door see the photo on the previous page For infor mation how to connect all eluent tubing to the pump head as well as on how to remove see the next chapters The possibility exists for a static mixing chamber to be affixed to the metal plate behind the front door of the pump The mounting threads item 3A in Fig 3 are used for this purpose Antec recommends using an eluent mixer with all gradient systems Pump head identification The front of the pump head is labelled with the max pumping capacity 10 ml or 50 ml Pump heads with inlays are additionally labelled with the re spective material Ti for titanium and C for ceramic The pump automati cally recognizes the pump head by means of an RFID chip see next para graph do kde DA CB CH Fig 4 A Labelling on pump head without inlays stainless steel B Labeling on pump head with titanium inlays A RFID The pump head is equipped with an RFID chip It is used to monitor and save all important parameters and settings of the pump and pump head In the display a diamond icon designates the RFID function A A load Pew Pwexsuse vente Peg min IMM oO ostin Proga P 00 75 000 p Setup Link OFF 000 00 100 0 O O P GLP Modo Tite A 26 WC wo Rus t rie Fig 5 Pump HFID designation in the pump display User manual LC 110S pump version 2 The RFID technology offers the following advantages e Automatic detection of all important pu
11. Maximum pressure stop Pmz 0 1 75 MPa switch off time lt 1 s Isocratic High Pressure gradient up to quaternary gradient 4 eluents Low Pressure gradient up to qua ternary gradient 4 eluents with optional Knauer 5000 HPLC manager Stand alone via Numerical keypad amp LCD 2 x 24 ch display Digital control via RS232C or LAN Analog control via REMOTE connector stop flow pressure error Report of number of pump cycles working time deliv ered volume and service information IP 20 Physical specifications Dimensions Weight 390 D x 226 W x 135 H mm 15 4 D x 8 9 W x 5 3 H 5 3 kg 11 7 Ibs Accessories and Spare parts APPENDIX 2 59 Accessories and Spare parts The following set of accessories is supplied with each LC 110S Partnumber 193 0400S 193 0402 193 0404 193 0406 193 0408 193 0410 193 0412 193 0414 193 0416 193 0418 193 0420 193 0422 193 0426 193 9428S Description LC 110S connector strips LC 110 LC 110S open ended IO cable LC 110 LC 1108 set for manual piston wash LC 110 LC 110S tool set LC 110 LC 110S power cable EUR LC 110 LC 110S power cable US LC 110 LC 110S RS232 cable LC 110 LC 110S syringe 10 mL LC 110 LC 110S 1 8 nut inlet LC 110 LC 110S 1 8 ferrule inlet LC 110 LC 110S PTFE eluent filter LC 110 LC 11068 ferrite LC 110 piston wash tubing 2 meter LC 110S LAN cable The following pump head p
12. Removing the check valves A Capillary connection B Outlet fitting C Check valve and D Inlet fitting The arrows indicate the flow direction of the check valve make sure to install the valves back with the correct flow di rection Cleaning the check valve Procedure 1 Putthe valve in a beaker with a suitable cleaning solvent iso propanol or ethanol 2 Putthe beaker in an ultrasonic bath and clean it for at least 10 minutes Installation of the check valve Note the inlet and outlet check valve are identical Make sure to in stall the check valves with the correct flow direction If installed in correctly it may lead to malfunction of the pump or damage to the wear and tear parts in the pump head Maintenance 51 Fig 40 Functional principle and flow direction of the check valve A Check valve B Ruby closing ball C Flow direction Note the ribbon on the side of the check valve cartridge this indicates the direction of flow Procedure 1 Insert the check valves in the bushing holes on the pump head 2 Screw in the inlet fitting and tighten with a wrench torque 8 Nm 3 Screw in the outlet fitting and tighten with a wrench torque 8 Nm 4 Screw in the capillary connection and tighten with a wrench Periodic cleaning of the device A e All smooth surfaces of the device can be cleaned with a lightly moistened cleaning clot with water e The display or touchscreen of the devices can be clean
13. To disconnect the pump from the mains power one need to toggle the mains on off switch on the rear of the device Pump head User manual LC 110S pump version 2 The pump head is the core element of a pump Pump head 10 ml for use in analytical applications standard ver sion made of stainless steel Titanium or ceramic inlays for bio compatible applications Pump head 50 ml for use in semi preparative applications stan dard version made of stainless steel Titanium or ceramic inlays for biocompatible applications 1A 1B 3A 1D 1c 2l 2H 2G 2F Fig 3 Pump head and pressure transducer with purge valve 2 2A 2B 2C 2D 2E 2F 2G 2H 2l Pump Head Piston wash inlet Piston wash outlet Capillary connection Piston wash connection Outlet check valve Pump head body Pump head mounting screw Eluent inlet with check valve Capillary conn to purge valve 1 1A 1B 1C 1D 3A Pressure Transducer Purge valve pressure transducer High pressure eluent outlet to injector De areation purge screw Purge outlet Mixing chamber optional Mounting threads for static mixing chamber The LC 110S pump is standard delivered with a 10 mL stainless steel pump head with Sapphire pistons and PTFE seals both high pressure and Description of instrument 5 wash seals The pump head all hydraulic connections including those of the optional integrated mixing chamber are located on the front side of the LC
14. a flashing rectangle Navigation through the main menu e Use the horizontal arrow keys to navigate through the two lines of the main menu s display e Use the number keys to enter a value Alternatively with the verti cal arrow keys you can increase and decrease the displayed val ues Operation FLOW d mL min 1 MI s L gt Fig 14 Display LC 110S main screen Menu A SETUP menu B GLP menu C LINK menu D PROGRAM menu Selecting the Setup menu Use the horizontal arrow keys to put the cursor in the top line of the display Press the right horizontal arrow key for approx 1 second Press START STOP or hold horizontal arrow key for 1 second to return to the main menu Selecting the GLP menu Use the horizontal arrow keys to put the cursor in the lower line of the display Press the right horizontal arrow key for approx 1 second Press START STOP or hold horizontal arrow key for 1 second to return to the main menu Selecting the Link menu Use the horizontal arrow keys to put the cursor in the lower line of the display Press the left horizontal arrow key for approx 1 second Press START STOP or hold horizontal arrow key for 1 second to return to the main menu Selecting the Program menu Use the horizontal arrow keys to put the cursor in the top line of the display Press the left horizontal arrow key for approx 1 second 16 User manual LC 110S pump version 2 e P
15. equipment of Antec Leyden which are subjected to the WEEE directive shipped after August 13 2005 are labelled with the crossed out wheelie bin The symbol on the product indicates that the product must not be disposed as unsorted municipality waste Collection amp recycling information business to business Antec Leyden offers the possibility for disposal and recycling of their instrument at an appropriate recycling facility if requested there may be costs involved with this service Please contact Antec Leyden for more information about this service and to register the return and disposal of end of life instruments info myantec com To assure hygienic amp personal safety all instrument should be returned with a signed decontamination form which is available on the website Shipping address for end of life products Antec Leyden Industrieweg 12 2382NV Zoeterwoude The Netherlands In case of questions or if further information is required about the collec tion amp recycling procedure please contact Antec or your local distributor ROHS directive The LC 110S is ROHS compliant and in conformity with Directive 2002 95 EC Restricted use of Hazardous Substances in electrical and electronic Equipment ROHS Antec Leyden is an ISO 9001 2008 certified company Intended use HPLC High pressure liquid chromatography HPLC is a method for separating substance mixtures determining substances and measuring thei
16. protective earth via a grounded socket using the power cord supplied by the manufacturer The power source should exhibit minimal power transients and fluctua tions Replace faulty or frayed power cords User manual LC 110S pump version 2 Laboratory regulations Observe national and international regulations pertaining to laboratory work e Good Laboratory Practice GLP of the American Food amp Drug Administration e For development of methods and validation of devices e Protocol for the Adoption of Analytical Methods in the e Clinical Chemistry Laboratory American Journal of Medical Tech nology 44 1 pages 30 37 1978 e Accident prevention regulations published by the accident insur ance companies for laboratory work Solvents Organic solvents are highly flammable Since capillaries can detach from their screw fittings and allow solvent to escape it is prohibited to have any open flames near the analytical system Regularly check for leaks and clogged LC tubing and connections Test back pressure without column Do not close or block drains or outlets Do not allow flammable and or toxic solvents to accumulate Follow a regu lated approved waste disposal program Never dispose of such products through the municipal sewage system Solvents suitable for use in HPLC e Acetic acid 10 50 at 25 C e Acetone e Acetonitrile e Ethanol e Ethyl acetate e Hexane heptane e lsopropanol e Methanol e Phosphoric
17. subsequently with water methanol Vacuum degassing Considerable amounts of the gases No O2 and CO may be dissolved in HPLC mobile phases Whenever the temperature changes solvents are mixed or a pressure reduction occurs these gases may show up as very small air bubbles To avoid noisy baselines an in line vacuum degasser has been integrated in the ALEXYS analyzer ROXY EC LC system It has a low dead volume less than 0 5 mL and high degassing efficiency The advantage over helium degassing is that the degasser does not change the mobile phase composition Helium degassing Degassing using helium is an effective and universally applicable method but only recommended when working in reductive electrochemical detec tion All gases except helium are removed completely Helium is not EC active and does not change the mobile phase properties significantly To prevent mobile phase contamination only high purity helium must be used xi Warranty conditions amp spare parts The factory warranty for the device is valid for 12 months after the date of dispatch All warranty claims expire in the event that any unauthorized changes are made to the device During the warranty period any compo nents with material or design related defects will be replaced or repaired by Antec Leyden free of charge This warranty excludes the following e Accidental or willful damage e Damage or errors caused by third parties that are not contractu
18. tests of the RAM memory motor unit and will perform an initial piston wash After completion the instrument will display the pump main menu and is ready for use User manual LC 110S pump version 2 Procedure 1 Switch the on the pump 2 Wait until the pump has completed the self test Operator errors and clogged capillaries can cause pressure spikes To avoid damage to the pump head and pump head seals never run the LC 110S without eluent in the pump head as well as in the rear seal washing compartment To set up the piston back wash follow the instructions in chapter 2 Avoid precipitation of buffer salts in organic solvents Always start with flushing system with water when switching solvents Never switch off the LC 110S with mobile phase in it as salts will precipitate an clog the system First flush with water subsequently with wa ter methanol Software control The LC 110S can be controlled by Clarity Chromatography software from DataApex For information about computer control with Clarity please refer to the Clarity user manual When working stand alone all operational parameters are set using the LC 110S keyboard See the section below Main menu For an explanation of all functions parameters on the display and keyboard see chapter 2 The main menu contains the current parameters of the pump From here the Setup GLP Program and Link menus can be accessed During navi gation through the display the cursor is
19. the freight carrier in case of damage otherwise they may not accept any responsibility The device is fixated and protected by foam inserts at the top and bottom Please keep the transport box and the foam inserts as it is designed for optimum protection during transport and it may be needed again Carefully unpack the instrument and inspect it for completeness and possible damage Contact your Antec supplier within three working days in case of damage or if not all marked items on the checklist are included To unpack the device remove the foam insert on the top of the device Grab the device firmly at its side near the front panel and lift it out of the trans port box A protective film is adhered to the LCD display to prevent scratch ing of the screen Remove the protective film from the display before use Prior to shipment your pump has been inspected and tested to ensure the best possible performance A certificate of performance is included in the ship kit The shipment consist of the following parts Partnumber Description 193 0035S LC 110S pump with installed pump head 193 0010 LC 110S user manual 193 0012 LC 110S Certificate of Performance 193 0400S LC 110S connector strips 193 0402 LC 110 LC 110S open ended IO cable 193 0404 LC 110 LC 110S set for manual piston wash 193 0406 LC 110 LC 1108 tool set 193 0408 LC 110 LC 110S power cable EUR 193 0410 LC 110 LC 110S power cable US 193 0412 LC 110 LC 110S RS232 cable 193 041
20. 1 jnnl Load fiow Peesere Deni Poog bi a tO la Prog af P 1 000 000 Setup Link OFF 000 00 100 0 P GLP Mao Tre A w Y wb Rent ir Load ro Peesere Deni Prag imm DUMP to oe 1 IL Prog P01 1 000 O00 p Setup Link HOLD 000 00 100 0 b GLP Modo Time A w Vc wo Rue 1 mm t no Pesamuaw Overix Posag immi a c0 oe 11012 Prog 4 P01 1 000 O00 pe Setup Link RUN 000 10 83 17 p GLP Modo Time wa w wo Run 1 mr Load fiow Peesere Deni Poog immi ey fO 0611012 Prog P01 1 000 000 p Setup Link END 000 50 0 100 p GLP Mold Tre wwe wo Run 1 mini Fig 30 Running a program 39 40 User manual LC 110S pump version 2 Modifying amp Deleting Programs Modifying a program To modify values in just enter new values and the old values are automati cally overwritten Procedure 1 Select the Program menu 2 Select the program number 3 Press horizontal arrow key to go to the editing mode of the sub menu 4 Enter the desired value 5 Position cursor on the diamond icon and press horizontal arrow key for approx 1 s to exit the submenu Load Fine Peame Denriu Prog rimin fOAMPa oto ca ttl Pragx Open PM Of pSetup Link 1 1 P GLP Hat Tiris An 4B Ww O Bart arr Load Fine Famzura Geert Prog rein OAMPa TO oot l2 Proga Paz 1 000 416 00000000 setup Linkw d no 0 10 80 D O jie GLF Hadt Tima El BO NO Bart arl Fig 31 Changing a progra
21. 110S pump version 2 Fig 21 tubing connections for piston backflushing Legend A From the storage container of the flushing solution to the inlet of the flush pump B From the outlet of the flush pump to the inlet of the piston back flushing C Outlet of piston backflushing D Outlet from the flush pump to the piston backflushing Tubing connections Low pressure eluent line Make sure that the tapered side of the cutting ring is pointed towards the fastening screw of the Teflon tube Fig 22 Low pressure tubing connection at the inlet of the pump head Procedure 1 Push the 1 8 Teflon or FEP tube D through the nut C and the ferrule B Operation 27 2 Insert the tube end as far as possible into the inlet fitting A of the pump head 3 Tighten the nut by hand High pressure tubing connections One piece disposable polyetheretherketone fittings PEEK in combination with PEEK tubing can be used for easy installation of flexible thin capillar ies for system pressures up to 300 bar The tightening torque of the PEEK screw tightened by hand approx 0 5 Nm The maximum pressure rating of PEEK tubing is 350 bar Above 350 bar always use stainless steel tubing in combination with stainless steel nuts and ferrules 28 User manual LC 110S pump version 2 CHAPTER 4 Installation guide Unpacking Inspect the transport box for possible damage as it arrives Immediately inform
22. 4 LC 110 LC 110S syringe 10 mL 193 0416 LC 110 LC 1108 1 8 nut inlet 193 0418 LC 110 LC 1108 1 8 ferrule inlet 193 0420 LC 110 LC 110S PTFE eluent filter 193 0422 LC 110 LC 1108 ferrite 193 0426 LC 110 piston wash tubing 2 meter 193 9428S LC 110S LAN cable Only use original parts and original accessories made by f manufacturer or a company authorized by manufacturer The Manu Installation guide 29 facturer will not accept any liability for damage direct or indirectly caused by the use of non original parts and accessories Installation The installation procedure consists of the following steps 1 Electrical connections 2 Tubing and fittings 3 Priming system with water Install the pump in an area which meets the environmental conditions and space requirements listed in Table Place the LC 110S on a flat and smooth surface or inside the special PR 110 pump rack module 192 0042 which is part of the ALEXYS ROXY LC EC system These are open metal stackable racks Table Environmental conditions amp space requirements Parameter Requirement Operating temperature 4 40 C 39 2 104 F Operating humidity below 90 non condensing Sunlight Do not expose the device to direct sunlight Side clearance At least 5 cm if there is another device on one side At least 10 cm if there are other devices on one both sides At least 30 cm gap to the fan on the rear of the Clearance back side devic
23. APTER 3 Operation Operating modes Isocratic e Analysis without gradients e The solvent composition is constant during the analysis e The solvent can be recycled Low Pressure Gradient LPG The gradient is formed on the low pressure side of the pump low pressure gradient X co nonG nacaono Fig 11 Low Pressure Gradient operating mode A HPLC manager with degasser and gradient module B Pump with pressure sensor and mixing chamber C Mixing chamber For operation in the Low Pressure Gradient mode an optional HPLC man ager with intake manifold is required Operation 13 Fig 12 Left side Intake manifold with 4 inlets A Knurled head screw B Filler cap C Mixing unit Right side Intake manifold mounted on inlet of pump head with 4 eluent lines connected High Pressure Gradient HPG The gradient is formed on the high pressure side of the pump high pressure gradient s nodoncoc naoaoanapo Fig 13 High Pressure Gradient operating mode A Pump with degasser module and pres sure sensor B Pump with pressure sensor and mixing chamber C Mixing chamber For operation in the High Pressure Gradient mode an optional static mixer is required Power On and Self test Upon powering up the instrument the display will present the text Antec LC 110S pump and the software version e g V00 XX or higher The instrument performs a self test including firmware validation and
24. C 110S pump head the following tool is required Partnumber Description 193 0364S LC 110S seal insertion tool Service of parts inside of the pump head seals pistons and O rings should only be performed by trained service personnel Replacement of seals and pistons by untrained end users can lead to loss of pump performance or damage to the pump head and is at the end users own risk and not covered by warranty What maintenance tasks may the user perform on the device Users may perform the following maintenance tasks themselves e Replacing the pump head e Exchanging the check valves e Cleaning the exterior Removing the pump head Perform the following procedure to remove the pump head 1 Purge the pump head with distilled water Danger of skin irritation may exist if aggressive toxic solvents remain in the pump head Wear protective gloves 2 Remove the tubes from the piston backflushing B 3 To remove the capillary loosen the screws at the pump head out let B and pressure sensor inlet A 4 Disconnect the eluent connection at the inlet of the pump head D 5 Using an Allen wrench loosen the opposite pairs of fastening screws C at the pump head alternately and evenly to prevent the pump pistons on the inside from jamming 6 Hold the pump head by hand and consecutively pull out all fasten ing screws 7 Remove the pump head carefully Maintenance 49 Fig 38 Removal of pump head
25. How to contact Antec Technical Support For technical support or questions please consult your local Antec distribu tor in your country When no distributor is available in your country contact the Antec main office or Antec US office for American end users The con tact address of your local distributor can be found on the Antec website www myantec com in the contacts section Furthermore the website contains a detailed knowledge base containing a large troubleshooting and maintenance section This section of the site is accessible for registered end users Registration can be performed online on the website Antec Main Office in the Netherlands Languages English amp Dutch Available by telephone 8 30 a m till 17 00 p m CET Phone 31 0 71 5813333 Fax 31 0 71 5813334 E mail support myantec com Antec LLC in the USA Languages Available by telephone English 8 30 a m till 17 00 p m CDT Phone 888 572 0012 toll free 781 935 5887 Fax F 321 821 1938 E mail sales usa myantec com Maintenance The following maintenance work on the device should only be performed by Antec service engineers or a company distributor authorized by Antec e Opening the device and service on the mechanics and electronics e Opening the pump head and servicing replacing pistons seals O rings or mechanical parts of the pump head 48 User manual LC 110S pump version 2 To be able to replace seals in the L
26. U DISPLAY Brighiness 2075 Contrast 0741 Fig 16 Structure of the SETUP menu cont Operation Parameter INTERFACE IP adr mask IP GW Port DATE PUMP HEAD PRESSURE LIMITS GRADIENT MODE LPG CYCLE TIME 19 Description Setting for external connection options of pump The connections are on the rear of the device Network configuration IP address Network Mask Network configuration Gateway Port Setting for the date and time Pump head selection The selection is used as basis for calculating the flow rate and determines the max system pressure This value applies for all operating modes and cannot be exceeded Settings for the min and max pressure The pump stops automatically if the pressure drops below the min value or exceeds the max value The min pressure value is used to ensure that the pump does not run dry If the mini mum is set 0 the minimum system pressure is not monitored Here the modes of the high pressure gradient HPG low pressure gradient LPG and iso cratic system can be set LGP activation of up to four channels HPG selection between pump name HPG A to HPG D Isocratic system none Time specification for the switching cycle of the valves of the Smartline Man ager 5050 Factory preset is 2 seconds When working with higher flow rates and lower mixing chamber volumes reduce the time to achieve better mixing of the eluents Whe
27. acid e Water Even small quantities of other substances such as additives modifiers or salts can influence the durability of the materials The list of selected sol vents were compiled based on research in the pertinent literature and is only a recommendation by the manufacturer In the event of doubt contact the technical support department of the manufacturer vii Unsuitable solvents The following solvents can attack the components of the pump and are therefore not suitable e Mineral and organic acids except in buffer solutions e Bases except in buffer solutions e Liquids containing particles Only suitable to a limited extent The following solvents are suitable to only a limited extent for use in the pump e Methylene chloride e Tetrahydrofuran THF e Dimethyl sulfoxide DMSO e Slightly volatile solvents e Fluorinated hydrocarbons Self ignition point Only use solvents that have a self ignition point higher than 150 C under normal ambient conditions Solvent tray To avoid damage from leaks always place solvent bottles in a solvent tray on the device Toxicity Organic solvents are toxic above a certain concentration Ensure that work areas are always well ventilated Wear protective gloves safety glasses and other relevant protective clothing when working on the device Operating the device or system The device can be operated by means of the chromatography software at the workstation the to
28. ally related to Antec Leyden at the time the damage occurred e Wear amp tear parts fuses glass parts amp columns e Damage caused by negligence or improper operation of the device and damage caused by clogged capillaries e Packaging and transport damage In the event of device malfunctions contact your local Antec representative or if not available in your country the Antec main office Antec Leyden B V Industrieweg 12 2382 NV Zoeterwoude The Netherlands P 31 0 71 5813333 F 31 0 71 5813334 E mail info myantec com Internet www myantec com Manufacturer provides operational spare parts of the instrument and cur rent accessories for a period of five years after shipment of the final pro duction run of the instrument Spare parts will be available after this five years period on an as available basis Manufacturer provides a variety of services to support her customers after warranty expiration Repair service can be provided on a time and material basis Contact your local represen tative for servicing Qualified chemists on both contractual and as needed basis can provide technical support and training The packaging of our devices provides the best possible protection against transport damage However immediately inspect each delivery for signs of transport damage If the shipment is incomplete or damaged inform the manufacturer within three workdays Also inform the freight carrier about transport damage
29. anel and foil key touchpad functions LCD display Keyboard B Program Link A Pump START STOP C Flow rate L Purge key D Pressure Numeric key pad E Event status H Arrow keys H Gradients G Standbye key J Run time K Status of pump program or Link The display shows information about the current status of the pump such as the flow rate the measured pressure or the current status of the running Description of instrument 3 program It also serves to display input values while running parameters are being modified e START STOP key A Once the flow rate has been entered and set the pumping process can be either started or stopped by pressing the START STOP key e The PURGE key L allows immediate access to the purging func tion of the pump e The numeric keypad I allows for the entering of running parame ters as well as for the control of program cycles e The arrow keys H allow to navigate horizontally and vertically through the menus Furthermore the vertical arrow keys can be used to increase and decrease the displayed values e The standby key G allows for the pump to be easily shut off in an energy saving mode as well as restarted By pressing down on the standby key for more than 2 seconds the pump will be completely shut down Once the pump has shutdown the standby key will light up and remain lit The pump can then be restarted by again press ing on the standby key for more than 2 seconds Note
30. arts are available for the LC 1108 Partnumber Description 193 0300S 193 0301S 193 0304S 193 0302 193 0303S 193 0326S 193 03248S 193 0308 193 0312 193 0358S 193 0360S 193 0342S 193 0362S 193 0316 193 0330S 193 0310 193 0346 193 0322 LC 110S pump head 10mL SS PTFE Sapphire LC 110S pump head 10mL SS PE Zirconium LC 110S HP pump head plate SS LC 110 LC 110S check valve LC 110S check valve seal LC 110S check valve nut 1 16 outlet LC 110S check valve nut 1 8 inlet LC 110 LC 110S piston seal HP PTFE LC 110 LC 110S piston seal HP PE LC 110S centering ring sapphire LC 110S adaptor ring SS LC 110S capillary connection SS LC 110S support ring LC 110 LC 110S O ring LC 110S LP pump head plate SS LC 110 LC 110S piston seal LP PTFE LC 110 LC 110S cover disc wash seals LC 110 LC 110S spring 193 0340S 193 0336S 193 0334S LC 110S spring retaining ring LC 110S spring retaining plate LC 110S retaining plate screw 60 User manual LC 110S pump version 2 Partnumber Description 193 0318 LC 110 LC 110S piston Sapphire 193 0320 LC 110 LC 1108 piston Zirconium 193 0350S LC 1108 capillary pump head transducer 250 1568 LC 110 LC 1108 1 8 nut inlet 250 1566 LC 110 LC 1108 1 8 ferrule inlet 193 0348 LC 110 LC 110S capillary piston wash 193 0352 LC 110 LC 1108 pressure transducer 193 0354 LC 110 LC 1108 purge valve screw PEEK 193 0356 LC 110 LC 1108 purge tubin
31. bing connections The next section describes the connection of all tubing connections and priming procedure of the LC 1108 For specific instructions about the LC connections in an ALEXYS analyzer or ROXY EC LC system please refer to the relevant section in the LC connection kit installation guide supplied with you re system To access the LC 110S pump head and purge valve slide the drawer of the PR 110 module to the front as depicted in the figure below Subsequently open the front door of the LC 110S by pulling it on the right side the hinge is locate on the left side of the door Fig 25 Accessing the LC 110S pump Installation guide Fig 26 Front side of LC 110S pump with open door 1 High pressure outlet 2 Piston wash inlet 3 Piston wash outlet 4 Low pressure inlet 5 Purge valve 6 Pump head assembly 7 Pulse damper inlet 8 Pulse damper outlet e The unit is equipped with an automatic piston wash unit Con nect the piston wash tubing silicone tubing O D 3 mm part 193 0404 to the piston wash inlet and outlet Fig 26 number 2 and 3 For aqueous mobile phases the wash liquid is usually water In case modifier is used in the mobile phase the wash liquid may contain 10 25 modifier e Priming piston wash Insert the tubing end connected to the inlet of the pump inside the bottle of wash liquid and connect a syringe to the other tubing end connected to the outlet Prime the piston wash flow path wit
32. contacts of the Events and Remote terminal strips Use manufacturer supplied cable s only to connect all I O s with other devices Thoroughly connect the shielding to common Manu facturer will not accept any liability for damage direct or indirect caused by connecting this instrument to devices which do not meet relevant safety standards Remote terminal strip e For receiving start control and error signals from external devices e For sending start control and error signals to external devices E m 5 A IDNUNII Fig 7 Remote terminal strip Description of instrument Signal Explanations ANALOG OUT Analog output signal Either outputs the measured sys tem pressure or a control voltage for Pump B The out put range can be set to values of max 1 2 5 and 10 V ANALOG IN Analog input signal for controlling the flow rate e g 1 V for 1 ml min in the case of the 10 ml pump head 1 V for 5 ml min in the case of the 50 ml pump head START OUT Output is active for 500 ms when the pump starts START IN Activated by a voltage of 0 V against GND ERROR OUT Output is active until the Error condition has been elimi nated ERROR IN A voltage of 0 V against GND stops the pump Events terminal strip For test purposes or to trigger control external devices e Sending control signals Events to external devices e Opening and closing contacts e Activating 500 ms pulses Load Flow Pressure Events
33. ddress of transmitter or receiver in local net work or Internet Internet protocol Combination of multiple chromatography programs in a single UHPLC system Mobile phase eluent carrier for liquid chromatography Low pressure gradient LPG Operating mode of an HPLC or UHPLC system The solvent is mixed on the low pressure side of the pump Combined device consisting of degassing module gra dient module and analog to digital converter The chromatography software controls the pump 62 Index A AC power source v access the LC 110 pump 32 accessories 59 61 B Buffer pH ix C certificate of performance 28 Clarity HPLC software 35 D degassing x drain 31 E EDTA ix Electrical connections 31 environmental conditions 29 EVENT terminal strip connections 7 F Front panel 2 H Helium degassing x high pressure gradient HPG 21 hydraulic connections 5 installation 29 lon strength ix K Keyboard 2 L LCD display 2 User manual LC 110S pump version 2 low pressure gradient LPG 21 M Mixing chamber 4 mobile phase requirements ix O ON OFF switch 7 Operating humidity 29 P passivation procedure 36 perating temperature 29 Power connector 7 PR 110 pump rack 30 Priming piston wash 33 PURGE key 3 Purge valve 33 R Rear panel elements 7 15 26 REMOTE terminal strip connections 7 ROHS ii RS232 data connection 31 S software version 13 solven
34. e Make sure that the power plug on the rear of the device is always accessible so it can be dis connected from the power supply Operate the LC 110S and all accessories according to the instruc tions and operating procedures in this manual This ensures proper operation and longevity of your equipment Use of this product outside the scope of this manual may present a hazard The Manufacturer will not accept any liability for damage direct or indirectly caused by use of the device outside the scope of the manual 30 User manual LC 110S pump version 2 Fig 23 Top PR 110 with stacker removed to insert pump Bottom PR 110 with stacker assembled arrows marking the allen screws to fix stacker on frame To insert LC 110S pump in the PR 110 pump rack perform the following steps see Fig 23 e Loosen Allen screws to remove the stacker e Insert the pump in the PR 110 frame The instrument feet should be fixed in the black supports on the base plate of the frame e Incase the pump is inserted properly the front of the door is aligned with the front side of the PR 110 frame e Fix the stacker back on the frame Installation guide 31 Connection of drain PR 110 rack Make sure that the silicone waste tubing is connected to the drain on the N back side of the PR 110 pump rack Fig 24 red circle to collect and dispose any excess of mobile phase in case the pump head is leaking The other tubing end should be placed i
35. ed and shipped back to the sender Exploded view pump head Fig 41 Exploded view of the pump head Maintenance 53 ID partnumber Description Qty 1 193 0304S LC 110S HP pump head plate SS 1 2 193 0302 LC 110 LC 110S check valve 2 3 193 0303S LC 110S check valve seal 2 4 193 0326S LC 110S check valve nut 1 16 outlet 1 5 193 0324S LC 110S check valve nut 1 8 inlet 1 6 193 0308 LC 110 LC 110S piston seal high pressure PTFE 2 7 193 0358S LC 1108 centering ring sapphire 2 8 193 0360S LC 1108 adaptor ring SS 2 9 193 0342S LC 110S capillary connection SS 1 10 193 0362S LC 110S support ring 2 11 193 0316 LC 110 LC 110S O ring 2 12 193 0330S LC 110S LP pump head plate SS 1 13 193 0310 LC 110 LC 1108 piston seal piston wash PTFE 2 14 193 0346 LC 110 LC 110S cover disc wash seals 2 15 193 0322 LC 110 LC 110S spring 2 16 193 0340S LC 1108 spring retaining ring 2 17 193 0336S8 LC 1108 spring retaining plate 1 18 193 0334S LC 110S retaining plate screw 2 19 193 0318 LC 110 LC 1108 piston Sapphire 2 20 193 0350S LC 1108 capillary pumphead transducer 1 21 250 1568 LC 110 LC 110S 1 8 nut inlet 1 22 250 1566 LC 110 LC 110S 1 8 ferrule inlet 1 23 193 0348 LC 110 LC 1108 capillary piston wash 2 54 User manual LC 110S pump version 2 CHAPTER 7 Trouble shooting Error messages The following error messages can appear in the LC 110S LCD display The messages are sorted alphabetically Table 2 Error mes
36. ed piston When trouble shooting is not successful and problems persist please con tact your local Antec distributor or the Antec main office for service Fur thermore on the Antec website www myantec com more troubleshooting information can be accessed via our Knowledge base To get access to the knowledge base please register yourself on the website 58 APPENDIX 1 User manual LC 110S pump version 2 Specifications LC 110S Pump General specifications Delivery system Wetted materials Power Operating conditions Flow rate Max pressure Flow rate accuracy Flow rate precision Pressure ripple Displacement volume Pressure protection shut down Operating modes Instrument control GLP features IP protection class Double piston pump with 10 mL pump head primary and auxiliary piston in a serial configuration automatic piston back flush PTFE Sapphire PEEK Stainless Steel Ruby and Ceramic with standard 10 mL pump head Auto sensing power supply 100 240V 47 63 Hz 40 W 4 40 C 39 2 104 F lt 90 RH non condensing 0 001 9 999 mL min 70 MPa up to 5 mL min pump head with metal inlays 40 MPa up to 10 mL min lt 1 5 0 2 2 mL min 0 196 1 mL min Residual pressure pulsation 3 H20 as eluent and P 12 MPa Primary piston 21 4 uL Secondary piston 9 7 uL Minimum pressure stop Pmin 0 off 75 MPa FS Switch off time 30 seconds
37. ed with iso propanol and wiped dry with a soft lint free cloth DANGER of electric shock or short circuit if liquid cleaning solution enters the inside of the device Cleaning cloth only lightly moisten Decontamination Contamination of devices with toxic infectious or radio active substances poses a hazard for all persons during operation repair sale and disposal of a device Danger caused by toxic infectious or radio active substances A con taminated device must never be submitted for repairs sold or dis posed of Contract a specialist company to decontaminate the device or perform the decontamination yourself if you have the required ex pertise All contaminated devices must be properly decontaminated by 52 User manual LC 110S pump version 2 a specialist company or the operating company before they can be re commissioned repaired sold or disposed of All materials or fluids used for decontamination must be collected separately and disposed of properly Procedure for return of devices or parts thereof for repair or maintenance at Antec main office 1 Call Antec front office for a Return Manufacturer Authorization RMA and quotation Without RMA the returned device will not be processed 2 Download a Decontamination form in PDF from the support sec tion of the Antec web site www myantec com Non decontaminated devices or devices not shipped with a filled in de contamination form will not be process
38. etup settings or change the gradient in the program or in setup Switch the device off and on If the system mes sage appears again notify Antec support Device error change device settings Change the entry Change the entry Check the cable connections Check the validity of the parameter s Trouble shooting Error message Invalid time in time table Invalid time table index Link is loaded Link is running Maximum pressure System stopped Minimum pressure System stopped No components are available in isocratic mode No link available No link available Pls edit link first No time table to start Non existing component is set to non 0 value Not enough space to store link Not enough space to store program Not supported Prg not compatible with pump head Program does not exist Program is running The gradient component is used this link is used in WAKEUP this program is used ina link this program is used in WAKEUP Time already exists Time table line is empty Too much lines in pro gram Unknown pump head 55 Remedy Correct the time entry Change the entry in the program line First unload the link then change the link or delete it Wait until the link has been executed then change the link or delete it Reduce the pressure or adjust the upper pressure limit Restart the system Increase the pressure or adjust the lower pressure limit Restart the system Since the pump can
39. fy Antec support Change the line index in the link Check the link number Values from 1 10 are pos sible Correct the entry Other troubleshooting information Power status LED off Possible cause No power Power off Remedy Plug in power cord if OR 110 is used check power on this device Switch on device using the on off button at the front panel No RS232 communication Possible cause No RS232 connection Wrong COM port as signed RS232 driver not in stalled correctly of the PCI COM cards Remedy Plug in RS232 cable s or replace faulty RS232 cable Reconfigure LC 1108 in the Clarity configura tion See Clarity software manual Re install the necessary RS232 driver from the installation CD supplied with the Serial port PCI connection kit p n 190 0202B and 190 0204B Excessive pump pulsations Possible cause Detector related problem Back pressure too low for pulse damper Check valve Seal Piston Remedy See detector trouble shooting guide For optimal dampening of flow pulsations a backpressure of at least 7 MPa 70 bar 21000 psi is needed Use backpressure regulator or restriction capillary between pulse damper and injector to increase pressure Clean or replace check valves Replace seal Remove pump head clean or replace pistons Trouble shooting 57 Leakage Possible cause Remedy Connectors Check connectors replace if necessary Leakage of seal Replace seal and or damag
40. g assembly The following power cables are available for the LC 110S The European and US power cable are parts of the LC 110S accessory kit the UK cable has to be ordered separately Partnumber 193 0408 193 0409 193 0410 Description LC 110 LC 110S power cable EUR LC 110 LC 110S power cable UK LC 110 LC 110S power cable US To be able to replace seals in the LC 110S pump head the following tool is required Partnumber 193 0364S Description LC 110S seal insertion tool Service of parts inside of the pump head seals pistons and O rings should only be performed by trained service personnel Replacement of seals and pistons by untrained end users can lead to loss of pump performance or damage to the pump head and is at his own risk and is not covered by warranty Abbreviations and terminology APPENDIX 3 61 Abbreviations and terminology Here you can find information on the abbreviations and terminology used in this device manual Terminology GLP Gradient HPG HPLC IP address LINK Solvent LPG Manager Remote Description Good Laboratory Practice quality assurance for labora tories Time dependent composition of solvent mobile phase on low pressure or high pressure side of system High Pressure Gradient HPG Operating mode of an HPLC or UHPLC system The solvent is mixed on the high pressure side of the pump High pressure liquid chromatography HPLC Unique a
41. h the syringe till all tubing is filled with wash liquid Subsequently insert the outlet in the bottle containing wash liquid Always connect the piston wash and make sure that the low pres sure compartment of the LC 1108 is filled with wash liquid The pis ton wash seals need to be lubricated at all time If not damage may occur of the wear and tear parts inside the pump head e Switch on the pump by means of the power switch on the back side of the unit The unit will initialize and the internal piston wash pump will start flushing automatically for a short time The pump will flush with regular intervals during operation Refresh the wash solution every week Check the piston wash fluid regularly in case the solution gets turbid or contains particles please refresh the liquid immediately The presence of black particles in the solution could indicate that the piston seals need to be replaced In 34 User manual LC 110S pump version 2 case the volume of the wash liquid in the bottle increased over time this could indicate leakage at the high pressure piston seals e Set the pump flow rate Pmin and Pmax to the desired value using the keypad of the LC 1108 e Connect the FEP tubing from the OR 100 degasser outlet as sembly between the outlet of the degasser and the inlet of the pump Fig 26 number 4 Insert the tubing in the slits on the aluminum frame of the PR 110 module to fix them e The solvent filter should be
42. ith respect to clinical analyses the instrument is intended for research purposes only While clinical applications may be shown this instrument is not tested by the manufacturer to comply with the In Vitro Diagnostics Di rective AN Symbols User manual LC 110S pump version 2 Where is it prohibited to use the device or system DANGER Risk of explosion Never use the device in potentially ex plosive atmospheres without appropriate protective equipment and approval by a notified body Inform the technical service department of the manufacturer Explanations of symbols amp labels on the device or in user manual Symbol Explanation Hazard symbol indicating microelectronic devices that can be damaged by electrostatic discharge when touched Flow direction symbol for piston back flushing inlet to flush pump Flow direction symbol for piston back flushing outlet from the flush pump Quality mark of a Nationally Recognized Testing Laboratory NRTL in Canada and the United States The certified device or system has successfully passed the quality and safety tests CE Conformit Europ enne mark for equipment that complies with the pertinent EU directives and comes with a declaration of conformity from the manufacturer The caution warning sign denotes a hazard It calls atten tion to a procedure or practice which if not adhered to could result in severe lethal injury or damage destruction
43. m Deleting program lines Procedure ONOaARWN gt Select the Program menu Select the program number Press horizontal arrow key to go to the editing mode of the submenu Enter the time value for example 000 7 Press horizontal arrow key Confirm the query To cancel the deletion process press any number LC 110S programs 41 Load Fire Pree Lwmrim Prag Dmi MPa eTO on 712 P M Hadt Tirra ua WB WO WO Run t mn Lendl Fire Prose Cerin Prog rismin fa eo se ILI Prag PUZ 1 000 0476 00000000 mSetup Linki d 000 5 O 100 D O GLP Hadt Tima WA WI uc BD Runt mn Load Plow Pros Everi Prag rr MPa oto on tLe Progag PO1 1 000 0476 00000000 Setup Linki d 000 7 O 100 D O i GLP Hadt Tira a WMI wc wb Run 1 Load Plow Pree Everi Prog risv n Pe TD on ILS Prag Delete prog line i peetup Unk confirm with upfdown key ie GLP Heed Tira WA ED BC WO Run t nm Fig 32 Deleting program lines Deleting a program Lond For Preme Car Preg bmj DOTqMP amp BT milL3 Mad Tim ua 4 SO MO IBun 1 rin t Fia FramLm CATE Prog mir Pe oof mildL2 Progeg Delete program P p Setup Link a b GLP Mad Tim wA W NO SD IBun 1 rin t Fa Pramrm Dat Prog rir Pa oot on JIL Prog 4 Delete program PD pesetup Link confsrm with cursor key b GLP iaaa Tima uA CE NO W Bun 1 mia Fig 33 Deleting a program 42 User manual LC 110S pump version 2 Procedure 1 Select the Program menu
44. manual LC 110S pump version 2 Flos Puma Dynix Poog frm fe cTO om 1 ILE Load Flos Fonera Eyenix Pegg linn pa cTO a 1 ILE Load File Fonera Lsuria Poag Denn MoO om Le Prog a LOW Sciup Link 4 imeli PO ROW WO ie GLP Hoi D Time THA Wi BO WO Aun 1 min Fig 35 Creating a link Running a link Procedure 1 Select the Link menu 2 Enter the link number Press horizontal arrow key to go to the editing mode of the sub menu Press START STOP to go to OFF mode Enter program number then press horizontal arrow key the display switches to HOLD mode Press number 1 The display switches to HOLD mode Press number 1 again The display switches to RUN mode linked program is running 9 After the linked program has been completed press START STOP The display switches to the OFF mode pa ONO Ss After the link has been completed the pump stops The display switches to the END mode LC 110S programs 45 load Pio Peesure Gverts Prog mme IMPs cTO oo tL gt 5 lIIII Ru Lika GLP Howo Tne wa s x wo L s Pov mmn j2 MPa cTO ain ier 1 i Prog t LO1 Setup Link line01 P02 R01 WO GLP Noa D Tie A SE c wb Rent ye toad how i Prog mme j2 MPa cTO oo tL Prog P02 1 000 000 p Setup Link HOLD 000 00 100 0 i GLP Noa D Ties A w uc wb Rant Load how Peesure l I Prog mmn j2 MPa cTO oo IL Prog P02 1 000 000 Setup Link
45. mp head parameters by means of radio signals radio frequency identification by the pump software or the chromatography software e Pump head type e Serial number and year of manufacture e Number of cycles and operating times e Limit values of the pump head parameters e Storage of all measuring data in accuracy with GLP Good Labora tory Practice e Transfer of all data to the software and pump display Description of instrument 7 Rear panel The rear of the device contains the mains power connection power switch power ratings connections for external devices markings and warning signs HPLC MANAGER G F E D C B Fig 6 Rear Panel of the LC 110S Rear panel elements ON OFF switch Power connector REMOTE terminal strip connections RS232 port LAN ethernet port EVENT terminal strip connections Socket for optional connection with HPLC manager for low pres sure gradient mixing Fan opening oQnmmuoou r I User manual LC 110S pump version 2 Electrical connections e Use the Events and Remote terminal strips to connect the pump with external devices e Use the RSS232 port to connect the pump to a computer e Alternatively use the LAN connection to connect the pump with ex ternal devices within a network e Use the HPLC manager connector to connect a Low Pressure Gradient mixer Caution Electrostatic discharge can damage the electronics of the pump Never touch the electronic
46. n working with lower flow rates and higher mixing chamber volumes increase the time to obtain more precise mixtures 20 SOLVENT EVENTS ANALOG OUT ANALOG OUT OFFSET PRESSURE OFFSET User manual LC 110S pump version 2 Data entry for the compressibility of the se lected solvent Water 0 46 Benzene 0 95 Chloroform 0 97 Ethanol 1 10 Acetone 1 23 Methanol 1 21 n heptane 1 40 n hexane 1 50 Diethyl ether 1 84 Acetonitrile 0 99 Tetrahydrofuran 0 93 Water methanol 10 90 1 17 Water methanol 20 80 0 86 Water methanol 40 60 0 56 Water methanol 50 50 0 52 Water methanol 60 40 0 46 Water methanol 80 20 0 40 Water methanol 90 10 0 34 Used for testing the Events terminal strip Connections E 1 to E 8 and 24 V can be se lected The lower line shows the statuses at the digital inputs Used to output analog pump signals to exter nal devices by means of the Remote terminal strip on the rear of the device Pressure preset for outputting the currently measured pressure for the purpose of exter nal recordings or control Slave B preset for outputting a signal for con trolling a second pump Presets HPG LPG A to HPG LPG D Voltage range options 1 V 2 V 5 V OFFSET data entry for the analog output Displays the automatic offset adjustment for the pressure display Make sure that the bleed valve is open Operation ANALOG IN TIME CONSTANT ACTIVATE EDIT EVENTS DISPLAY
47. nto a waste bottle which is placed lower then the PR 110 rack to assure proper drainage Fig 24 Rear view of LC 110S in the PR 110 pump rack The drain is encir cled in red Execute periodic leak checks on LC tubing and connections Do not allow flammable and or toxic solvents to accumulate Do not close or N block drains Follow a regulated approved waste disposal program Never dispose of such products through the municipal sewage sys tem Step 1 Electrical connections Mains e In case the pump is not used in combination with an OR 110 or ganizer rack connect the mains outlet of the pump to a mains wall socket using the LC 110S power cable EUR p n 193 0408 or US 192 0410 supplied in the LC 110S accessory kit see Fig 24 e In all other cases connect the mains outlet of the LC 110S pump and the OR 110 organizer rack using the OR110 LC 110S power cord p n 250 0144 supplied in the OR 110 accessory kit RS232 data connection 32 User manual LC 110S pump version 2 e To make a serial data connection use the LC 110S RS232 ca ble p n 193 0412 supplied in the LC 110S accessory kit Connect it to RS232 connector 1 see Fig 24 and a free PC COM port or USB to COM converter port For more detailed information about setting up a serial connection for the LC 1108 in a complete ALEXYS ROXY system please consult the installa tion checklists p n 180 0011A and 190 0011B supplied with your system Step 2 Tu
48. only Only allow the technical service department of the manufacturer or a company authorized by the manufacturer to open the devices for maintenance and repair work Mobile Phase Requirements LC EC Electrochemical detection is a sensitive detection technique characterised by extremely low detection limits A typical detection limit of 100 pmol L or lower for catecholamines is no exception Improving detection limits will always be limited by the weakest link in an LC EC system Therefore in the ALEXYS analyzer or ROXY EC LC system all hardware is carefully selected to warrant optimum performance In daily practice a couple of rules must be obeyed to fully exploit the in credible linear dynamic range and low detection limits of an EC detector These are not only hardware related but also refer to mobile phase com position degassing temperature and pH stability and several other issues Mobile phase requirements e Electrochemically clean HPLC grade or better e lon strength 20 200 mmol L e Buffer pH near pKa e in line 0 2 um filter amp degassing of mobile phase e EDTA for trapping of metal ions Mobile phase must be clean as it is in direct contact with the working elec trode in EC detection High purity chemicals including water is a pre requi site In some applications EDTA is added to the mobile phase to traps electrochemically metals such as Fe by forming an inactive complex However at higher working potentials ty
49. only be operated using a spe cific valve the entered data has to be adapted to isocratic mode Create a link and edit it Create a link and edit it Edit the data by means of the chromatography software Switch on the channel or edit the data using the chromatography software Check the pump Check the number of program lines A maximum of 100 program lines are possi ble Check the pump Check the number of program lines A maximum of 100 program lines are possi ble Change the entry Modify the program or replace the pump head Create and edit a program Quit program or wait until program has been com pleted The setup data can only be edited when no pro gram has been loaded or started First unload the program First quit or delete wakeup program wu wakeup then edit or delete link First pause or delete the link then edit or delete data by means of the chromatography software First quit or delete wakeup program wu wake up then edit or delete data by means of the chro matography software Correct the time entry Edit the program line Check the number of program lines A maximum of 100 program lines are possible Check the pump head Check whether a pump 56 Error message type used CRC failed Wrong Line number Wrong link User manual LC 110S pump version 2 Remedy head with RFID recognition has been installed Switch the device off and on If the system message appears again noti
50. pically gt 1 2 V vs salt bridge AgCI ref also EDTA can become electrochemically active and is not recom mended In that case only a passivation step with 15 HNO is recom mended see installation guide Electrolytes assure contact between 3 electrodes in an electrochemical flow cell Low ion strength destabilises an EC system and noise will in crease Extremely high buffer concentrations cause problems of salt for mation Therefore concentrations between 20 and 200 mmol L are recom mended Also constant pH is important for baseline stability and reproducible results Stability of pH is best when close to pK of a buffer ion Often used buffers are phosphate carbonate acetate and citrate Modifiers such as methanol propanol and acetonitril can be used without problems in DC amperometry but not in pulsed amperometric detection as peaks are strongly attenuated In our experience the quality and expiration date of organic modifiers can be a problem resulting in increased noise levels Metal inlet frits in mobile phase bottles are not advised as metal ions are introduced that increase baseline noise Instead 0 2 um nylon inlet filters are recommended User manual LC 110S pump version 2 Avoid precipitation of buffer salts in organic solvents Always start with flushing system with water when switching solvents Never switch off the LC 110S with mobile phase in it as salts will pre cipitate an clog the system First flush with water
51. placed between degasser and mo bile phase reservoir only water or MeOH water 20 80 v v mixture for first use part 180 0204 Never use a metal inlet fil ter in mobile phase bottle Fig 27 In line filter arrow indicates flow direction The white knob on top is an air vent which should be opened for filling the filter with mobile phase Read specifications on solvent filter Aqueous type of filters are NOT suitable for high percentages modifier and are NOT acid resistant Use another type of filter in these cases e Connect the pump outlet Fig 26 number 1 to the pulse damp ener using a piece of 50 cm orange PEEK ID 0 5 mm or the cor responding tubing assembly OR 100 pulse damper inlet assem bly from the LC connection kit e Depending on your configuration connect pulse dampener to a manual or electrically actuated injector or auto sampler Consult the manual of auto sampler and detector for further installation in structions e Make sure all connections are leak free Step 3 Priming system To prime the system execute the following steps Installation guide 35 Position the mobile phase bottle at a height well above the level of the pump In case of an ALEXYS ROXY system in the dedicated SR 110 solvent rack on top of the pump Open purge valve Fig 26 number 5 The FEP inlet tubing should fill itself soontaneously with mobile phase due to gravity If it doesn t please check if the inline fil
52. r concentration The device or system is suitable for high pressure liquid chromatography It is suitable for laboratory use for analyz ing substance mixtures that can be dissolved in a solvent or solvent mixture Pumps The LC 110S pump is a conveying system for analytical and semi preparative applications Pump head inlays made of different materials cover all needs of the user If biocompatibility is required titanium or ceramic inlays can be used The pump head can operate at liquid pres sures up to a maximum of 650 bar The pump heads can be exchanged by the user Automatic piston back flushing increases the service life of the seals and pistons and removes salt and other substances from the area behind the seals Room ventilation AC system sunlight Always use the device in rooms that are well ventilated and are preferably equipped with an air conditioning system When setting up the system at the installation loca tion make sure that it is protected against direct sunlight Check intended use Only use the device for applications that fall within the scope of the specified intended use Else the protective and safety equipment of the device could fail Due to the new drive used in the pump pump heads of older series cannot be used any more Laboratory use e Biochemistry analyses e Chiral analyses e Food analyses e Pharmaceutical analyses e Environmental analyses e Clinical analyses research purpose only W
53. ress START STOP or hold horizontal arrow key for 1 second to return to the main menu SET UP menu In the Setup menu fundamental parameters for controlling the pump are specified Navigating through the Setup menu e Use the horizontal arrow keys to navigate through the two lines of the display e Use the vertical arrow keys to select the desired option e Move the cursor onto the diamond icon on the left in the lower line to navigate through the subitems of the Setup menu by means of the vertical arrow keys e Use the number keys to enter a value e Press START STOP or hold horizontal arrow key for 1 second to return to the main menu Operation Analog RS232 9600 es See ao RS232 115200 LAN manual DATE 19 01 2010 d m y 13 03 55 h m s PUMP HEAD a 10mi e Ceramic 10m PRESSURE LIMITS 0 1MPaj min 000 max 700 GRADIENT MODE none iD 0001 LPG CYCLE TIME 1s SOLVENT E 5 Compressibiity 0 34 EA EVENTS active start error Fig 15 Structure of the SETUP menu 17 User manual LC 110S pump version 2 Disabled HPGILPG D HPGILPG C HPG LPG B HPGILPG A Slave B ANALOG OUT pressure range 1 V ANALOG OUT OFFSET DODD ODmV PRESSURE OFFSET 00 00MPa ANALOG IN setzen actual 00 00 m TIME CONSTANT analog 10 5 in na nut na 55 25 is 0 65 O25 Activate edit events DODODOD
54. s and ROXY EC LC systems The pump has a double piston design with an advanced pulsation dampening mechanism assuring the delivery of solvents with very low residual pulsations and smooth baselines With its improved motor drive and pump head design it is capable to operate at back pressures up 700 bar The LC 110S has a versatile 10 mL pump head which can deliver flow rates in the uL range up to 10 mL min High pressure gradient as well as flow rate programming is supported Because the use of buffers and salts is indispensable in LC EC the LC 110S is equipped with an automatic pis ton back flush module to maximize the piston and seal life span The LC 110S can also be used as a high quality stand alone pump to be incorporated in any LC system Fig 1 ALEX Ys Analyzer ROXY EC LC system equipped with two LC 110S pumps Before installation of the device Chapter 4 read the safety instruc tions intended use section Chapter 2 Description of the device and Chapter 3 Operation carefully 2 User manual LC 110S pump version 2 CHAPTER 2 Description of instrument Front panel The figure below shows the front view of the LC 110S The keyboard and LCD display are located on the front side of the door Display panel and foil key touchpad functions are listed in the table The pump head pressure transducer and capillaries are located behind the front door L K J l H G Fig 2 Front view of the LC 110S Display p
55. sages Error message Auto pump head type head data uninitial ized Auto pump head type no head detected Cannot delete active program link Cannot edit program from the running link Cannot initialize LAN Cannot operate with an empty link Cannot read data from FRAM Cannot read RTC Cannot start time table Cannot use non existing component Cannot write data on FRAM Error input activated Insufficient access Invalid command Invalid parameter s Remedy Switch the device off and on Check whether a pump head with RFID recognition has been in stalled Repeat the automatic configuration in the chromatography software Remove the pump head clean it and install it again Switch the device off and on Repeat the automatic configuration in the chromatography software Re move pump head clean it and install it again Check whether a pump head with RFID recognition has been installed Pause active program or link Afterwards delete the link First pause the link then edit the data on the de vice display or in the chromatography software Check the cables and connections in the local area network The link is empty First create a link Switch the device off and on If the system mes sage appears again notify Antec support Switch the device off and on If the system mes sage appears again notify Antec support Edit the data at the device display or in the chroma tography software Change the s
56. se the number keys to enter a value e Press START STOP or hold horizontal arrow key for 1 second to return to the main menu e With the vertical arrow keys you can increase and decrease the displayed values Creating a Program The use of the display is demonstrated using the example of a program to be created Program 01 is to be used to create a solvent gradient chan nels A and B with constant flow rate of 1 ml min The percentage of sol vent A is to be continuously decreased from 100 to 0 within 0 50 min utes Procedure 1 Select the Program menu 2 Select the program number User manual LC 110S pump version 2 3 Press horizontal arrow key to go to the editing mode of the sub menu Enter the value for the flow rate 01 00 Enter the value for channel A 100 Enter the value for channel B 0 To specify the time in the submenu position the cursor on the diamond icon and press the vertical arrow keys 8 Enter the time value 000 5 9 Enter the value for channel A 0 10 Enter the value for channel B 100 11 Position cursor on the diamond icon and press horizontal arrow key to exit the submenu po mo Lond no Pressure Dens Prog Dmi 0 MPw EO oe 1 l2 Prog j Open P Lnks b GLP Mold Tee A wD wc wo Ron 1 mni t fiw Pressure Donis Pocs finn ee FO L2 Prog 4 Open Pot Setup Linka 2 a ee i GLP Motd Tere xa wo wo wo fon 1 mri t fiw Press
57. t filter 34 standby key 3 START STOP key 3 T trouble shooting 56 W warning sign iv wash liquid 33 WEEE ii
58. te terminal strips can some times cause electromagnetic interference This can be suppressed by means of a snap ferrite p n 193 0422 supplied in the ac cessory kit To attach the ferrite open the snap ferrite place it around the flat ribbon cable and close it Fig 10 Flat ribbon cable A with snap ferrite B attached Pump control The pump can be controlled individually or as part of a high pressure gra dient system HPG or low pressure gradient system LPG by means of a computer and chromatography software or standalone via the key board display on the front of the device For digital control via PC with chromatography software two communica tion ports are available e ARS232 communication port for serial control e An Ethernet connector for control via a local Area Network LAN For both types of connections a communication cable is supplied in the LC 110S accessory kit The pump uses an open control protocol which is available on request Clarity chromatography software DataApex Czech Republic is used for digital control of the LC 110S pump when the device is delivered as a part of an ALEXYS LC EC system or ROXY LC EC system LAN At the factory the pump is set to DHCP Dynamic Host Configuration Pro tocol This means that the pump is automatically assigned an IP address within a local network In the Setup menu this setting can be modified manually 12 User manual LC 110S pump version 2 CH
59. tem e Inthe Setup mode of the pump select Gradient Mode HPG A HPG B HPG C or HPG D or none e n purging mode set the values for the flow rate Operation 25 Procedure Open the purge valve Press PURGE Enter the value for the flow rate Press START STOP to start the purging process Press START STOP to stop the purging process Qu RO m Load Fipar Pree Emir Prog rrr MMPs ato co atl Pmg4 PURGE 3 000 ODD a5MPa Setup Link 4 OFF I GLP Halo Tira A WB BC W0 Fun 1 mia Load Pio Pramurm Eie Frog Dmi IMPs atO oot JL Prmg4 PURGE 3 000 ODD 5MPa eSetup Link a PURGE b GLP Had Tima WA wn wc wn Fun 1 ris Fig 20 Purging the pump in the HPG or isocratic system Piston backflushing The pump is equipped with automatic piston backflushing It increases the service life of the seals and pistons and removes contaminants from the area behind the seals Functional principle The piston backflushing function automatically flushes the rear piston area of the pump head upon switch on and in continuous mode e Upon switch on The rear piston area of the pump head is auto matically flushed for 8 seconds e n continuous mode The rear piston area of the pump head is flushed automatically every 2 hours for 15 seconds The flushing interval can be changed in the chromatography software Recommended flushing solution The recommended piston wash solution is 1596 iso propanol 26 User manual LC
60. ter is clogged or in case of a new filter wet the filter with a small amount of MeOH water solution 50 50 v v At the moment the solvent supply lines are filled with solvent press the purge key to enable rapid self priming When primed with sufficient liquid stop purging and shut the purge valve apply only gentle force Check if the correct flow rate is set and start pumping by pressing the Run Stop key The actual pump pressure is displayed in the top line of the LCD display Clarity HPLC software control For control via the Clarity data system software stop the flow Run Stop and open the control driver window in Clarity The actual flow rate when not running a method single injection or sample sequence can be altered by changing the stand bye flow make sure the Standbye checkbox is active By pressing the apply button the entered flow rate will become active Method Setup C Clarity 2D_neurotransmitters 2D_FLinj_080228 x Select LC LC110 z Iv Enabled Knauer KPump LC Method Ausiliary Pump Time Flow i min mimin Standby Flow 0 05 ml min 1 0 05 Time to Standby 0 min 2 Standby Time min r ldle State C Pump Off C Initial Standby Options pus LC Status Description Status Demo Mode Ready LC Integration Calculation amp dvanced 3 Cancel Apply Report Help Fig 28 Control of the LC 110S from the Clarity data system soft
61. the high or low pressure gradient i e HPG or LPG you need to specify the channels used by each of the sol vents LPG Choose up to 4 channels valves from A to D HPG Choose names for each pump from HPG A to HPG D e Move to the second line by pressing the key and choose valves A to D for LPG mode or names for each pump actually present for HPG mode for each of the corresponding positions by press ing either the A or V key It is important to turn ON channels indi cating gradient composition e g 96A B C 96D for all pumps which are set in HPG mode e g if 2 pumps will be used and named as HPG A and HPG B channels corresponding to both pumps 96A and 96B should be ON in both pumps See Fig 17 This selection will apply for programs and define the num ber and names of solvents used in any gradient Fig 17 Pump display when gradient mode for 2 pumps HPG A and HPG B is properly set The first pump was configured as HPG A and both channels indicat ing A and B are ON GLP Menu The GLP menu reports statistical data for essential parameters of the LC 110S The figure below gives an overview of the GLP menu e Move the cursor onto the diamond icon to navigate through the subitems of the GLP menu by means of the vertical arrow keys e Press START STOP or hold horizontal arrow key for 1 second to return to the main menu Operation GLP P S N CAAD9 1800007 version 00 53 GLP P volume 0002 21
62. type 193 to which this declaration relates is in conformity with the following direc tives amp standards Safety Low voltage directive 2006 95 EC Safety requirements for laboratory equipment EN61010 1 08 2002 Class I Installation cat Il Pollution degree 2 Electrical accessories Cord sets and intercon EN60799 06 1999 nection cord sets Electro Magnetic Compatibility directive 2004 108 EC EMC requirements for electrical equipment for EN61326 1 10 2006 measurement control and laboratory use Harmonic current emissions EN61000 3 2 ROHS directive Restricted use of Hazardous Substances in electrical and electronic Equipment 2002 95 EC WEEE directive Waste electrical and electronic equipment 2002 96 EC Attention The product was tested in a typical configuration Use manufacturer supplied cable s only to connect all l O s with other devices Thoroughly connect the shielding to common Manufacturer will not accept any liability for damage direct or indirect caused by connecting this instrument to de vices which do not meet relevant safety standards The mark of conformity has been applied to the rear panel of the device November 28 2012 User manual LC 110S pump version 2 WEEE directive Antec Leyden is a Business to Business producer of analytical analysis equipment which fall under WEEE Annex IA categories 8 and 9 includes medical devices and monitoring and control instruments All
63. uch screen or the membrane keyboard at the device What expertise should users have to safely operate a HPLC device or system e Completed degree as chemical laboratory technician or compara ble vocational training e Fundamental knowledge of liquid chromatography viii User manual LC 110S pump version 2 e Participation in an installation of the system performed by the manufacturer or a company authorized by the manufacturer and suitable training on the system and chromatography software e Basic knowledge of Microsoft Windows e Knowledge regarding substances that are suitable only to a limited extent for use in liquid chromatography To what should the user pay particular attention To make your HPLC separations as efficient as possible pay close atten tion to the following e Avoiding additional dead volumes o Once they have been used never re use capillaries in other areas of the HPLC system o Only use a given PEEK fitting for one specific port and never re use it for other ports Al ways install new PEEK fittings on each separate port e When using special columns obey the manufacturer s instructions on caring for the columns e Regularly check for clogged capillaries test back pressure with out column e Using filtered solvents o Use ultra pure filtered solvents for HPLC o Filtration of substances under analysis o Use of inline filters Devices are to be opened by the technical service department
64. ure Dveris Prog Gmin ey oO oe 1012 Prog 4 P01 1 000 0418 00000000 pSetup Linka e 000 0 100 0 O O GLP Mold 0 Tree A B owc wo Ron 1 mei t fiw Presere Dveris Prag finn ey FO oe 1112 Prog PO1 new time Setup Link 000 5 i GLP Mold Tree A B wc wp Ron 1 mr Load fiow Pressure Dveris Peg Dui 2 WPw oFO La Prog P01 1 000 0200 00000000 pmSetup Linka 4 0005 0100 0O O m GLP Mold Tree A B wc wo Ron 1 mri Fig 29 Display of single steps when creating a program Running a Program During the program sequence the changes in the solvent composition are displayed in the A 96 D fields After the program sequence has been completed the display switches to END mode LC 110S programs Procedure e m nons Select the Program menu Select the program number Press horizontal arrow key to go to the editing mode of the sub menu Press START STOP the display switches to OFF mode Enter program number then press horizontal arrow key the display switches to HOLD mode Place the cursor onto HOLD and press 1 the display switches to RUN mode program is running After the program has been completed the display switches to END mode Press START STOP Prog Open POM Setup Link ae p GLP Mold o Tre w wc wo Rent ir Load Pos Peesere Deni Poog b oO 0112 Prog P01 1 000 0416 00000000 Setup Link 000 0 100 0 O O jP GLP Modo Tre A w Vc wo fen
65. ware 36 User manual LC 110S pump version 2 The pump is now ready for use Continue with installation procedure of other parts of HPLC system following instructions in corresponding manu als In combination with electrochemical detection a passivation step with 15 HNO is recommended see installation guide of the detector and in case of a complete ALEXYS analyzer in the LC connection kit installation guide First remove all parts that are not acid resistant such as nylon inlet filters column and flow cell See installation guide of detector for passivation procedure with 1596 HNO3 LC 110S programs 37 CHAPTER 5 LC 110S programs The LC 110S can be programmed to perform the following actions e Control of flow rates e Control of solvent compositions e Output of control signals Events The LC 110S can store up to twenty programs with a maximum of 100 program lines in total Up to nine links can be created between existing programs Navigating through the Program menu A diamond icon is displayed on the left of the lower line in the display Dur ing data entry the cursor turns into the underscore character e Use the horizontal arrow keys to navigate through the two lines of the display e Use the vertical arrow keys to select the desired option e Move the cursor onto the diamond icon on the left in the lower line to navigate through the sub items of the Setup menu by means of the vertical arrow keys e U
66. xii User manual LC 110S pump version 2 Table of contents Declaration of Conformity i WEEE ROHS directive ii Intended use iii Symbols iv Safety practices v Warranty conditions amp spare parts xi Introduction 1 Description of instrument 2 Front panel 2 Pump head 4 Rear panel 7 Electrical connections 8 Pump control 11 Operation 12 Operating modes 12 Power On and Self test 13 Software control 14 Main menu 14 SET UP menu 16 GLP Menu 22 Purging the pump 24 Piston backflushing 25 Tubing connections 26 Installation guide 28 Unpacking 28 Installation 29 Step 1 Electrical connections 31 Step 2 Tubing connections 32 Step 3 Priming system 34 LC 110S programs 37 Creating a Program 37 xiii Running a Program 38 Modifying amp Deleting Programs 40 Wake Up Program 42 Link menu 43 Maintenance 47 How to contact Antec Technical Support 47 Removing the pump head 48 Installing the pump head 49 Exchanging the check valves 49 Period cleaning of the device 51 Decontamination 51 Exploded view pump head 52 Trouble shooting 54 Error messages 54 Power status LED off 56 No RS232 communication 56 Excessive pump pulsations 56 Leakage 57 Specifications LC 110S Pump 58 Accessories and Spare parts 59 Abbreviations and terminology 61 Index 62 Introduction CHAPTER 1 Introduction Congratulations on your purchase of the LC 110S HPLC pump The LC 1108 is a vital component of the ALEXYS analyzer

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