Ettan Spot Handling Workstation
Contents
1. Reagent Catalog l Workstation id seuppw000086 Software version 2 1 System SpotPicker Digester I Spotter CS System Service Configuration Place the marker on the picker head and press start to start the alignment iteration Method Management E Alignment status Not aligned Iterations 2 3 Batch Configuration Status Not performed Not p Batch Processing User Administrator Max spots 152 Total Confirm that the marker is placed on the picker head Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 57 4 User Maintenance 8 Click the Start button WARNING MOVING PARTS Do not enter the Spot Picker amp Spotter s AN working area directly after clicking the Start button since the picker spotter head and camera assembly can make sudden rapid movements The status bar is yellow during processing and turns into green when the camera alignment is completed Workstation id F8 2 Software version 2 1 Spot Picker Digester I Spotter Camera Alignment System Service Close Place the marker on the pick head and press start to start the alignment iteration Alignment status Not aligned Iterations 1 2 3 Status Complete Not performed T C Confirm that the marker is placed on the pick head Caen 1 save 9 Open the cabinet door 58 Ettan2. barcode reader while performing user maintenance inside the cabinet Output signals from the barcode reader will be interpreted by the Spot Picker amp Spotter and Digester units as a command to move to their home positions WARNING MOVING PARTS Do not read any barcodes with the external and preventive maintenance should be performed by qualified personnel 1 WARNING NO SERVICEABLE PARTS INSIDE Do not open covers Service Contact your GE Healthcare representative for more service information 4 1 System administration A competent person at the laboratory should be trained and appointed as system administrator for Ettan Spot Handling Workstation The system administrator should be responsible for the results and data backup Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 49 4 User Maintenance 4 2 User interface Parts of the user maintenance are handled from the System Configuration window see Fig 4 1 below ET Ettan Spot Handling Workstation SES File System BatchRun Reports Window Help System Configuration Reagent Catalog Workstation id seuppwoo0086 Software version 2 1 SEE Ed Digester Spotter Camera Alignment System Service Close Configuration a 5 Method Management volume REES Flow rates aD Rinse 0 5 ml Aspiration 20 ml min Dispensing 20 ml min Batch Rinse 30 ml min Configuration Batch Proce
3. 3 Rinse the buffer lines with Milli Q water or equivalent using the prime syringe tool 4 4 2 Gel trays When a batch run is completed 1 Remove the gel from the gel tray and store discard as appropriate 2 If the gel is to be discarded scrape the gel off the glass plate with a plastic spacer or similar object Then put the plates into a 5 Decon solution or equivalent overnight to remove any gel fragments which adhere to the plate 3 Rinse the liquid out of the gel tray with Milli Q water or equivalent and leave the gel tray to dry 4 4 3 Microplate lids 1 Wipe the lid with a soft damp tissue If needed use a mild detergent 2 Rinse the lid with Milli Q water or equivalent and let dry 3 Carefully insert the lids into the incubator Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 4 5 Camera alignment barcode reader while performing user maintenance inside the cabinet Output signals from the barcode reader will be interpreted by the Spot Picker amp Spotter and Digester units as a command to move to their home positions 1 WARNING MOVING PARTS Do not read any barcodes with the external 4 5 1 Required accessories Following accessories are needed for the camera alignment e Gel tray e Camera alignment foot code no 18 1143 33 e White paper e Double sided adhesive tape Alignment position Camera alignment foot Gel tray Gel tray support F
4. 60 0 k0 s 0e02 gt swmwsswesesssmmm 60 4 6 1 Changing procedure nansersssssssssssserersssesssssssserersssesssssssserersssssssssns 61 4 7 Changing the digester needles s 141191115111511151115111011 1511101 68 4 7 1 Changing procedure sesura ana 68 4 8 Changing picker head nnnsemeneeeeseres 73 4 8 1 Changing procedure 49 PANO 4 9 1 Priming the Digester 49 2 Priming the Dicker 49 3 Eil ellene AOA SUSLEM PRIMING sees Ee eer 4 10 Changing SYringes sssssserrrsvsvsssssssserersssssssssssserrrsssesssssssserersssssssssns 4 11 Preventive Maintenance E 82 5 Troubleshooting Specifications 6 1 Technical SPECIFICATIONS nsmmrsssssserrrsvoessssssssererrserses 85 6 2 Performance Specification m ssmsmsmsmsmsmsmsmsmsm 86 6 3 Regulatory requirements 6 4 Ordering information sssini 6 41 DEE IEN 6 4 2 Related productS nmmmmerrseevsssssssserrrrrsessssssssererrreesssssssssrernsennnr Index eeng Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD List of figures List of figures Fig 1 1 Ettan Spot Handling Workstation emmmsmsmsmsm 11 Fig 1 2 Ettan Spot Handling Workstation configuration 12 Fig 1 3 Rating label layout mseemessseeessseesssesevoi Fig 2 1 Ettan Spot Handling Workstation Fig 2 2 Reagent waste bottles and syringe pump module 18 Fig 2 3 The cabinet s lower left compartment m m Fig 2 4 The cabinet s lower right com
5. Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 83 5 Troubleshooting 84 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 6 Specifications Specifications 6 For complete specifications of each component refer to the individual hardware documentation Relevant system specifications are listed below 6 1 Technical specifications Table 6 1 Ettan Spot Handling Workstation technical specifications Ettan Spot Handling Workstation Mains voltages Operating temperature Dimensions Total weight Power consumption Sample source Maximum gel size Minimum gel size Gel thickness compatibility Spot picker head diameter Detection system compatibility Barcode reader Max output radiation Emitted wavelength Max beam divergence 100 120 230 240 V AC 10 15 50 60 Hz 2 Hz 18 30 C 65 86 F 251 5 x 129 x 198 5 cm W x D x H 645 kg 1600 VA Polyacrylamide gels cast on glass or GelBond PAG film backing 276 x 212 mm 180 x 160 mm 1mm 1 4 2 0 mm Silver Coomassie fluorescence 1 2 mW 223 us 670 nm 60 degrees Ettan Spot Handling Workstation System Ma
6. 0 5 mm above the calibration knob Adjust the XY position against the center of the calibration knob The tip of the needle must point precisely to the center of the calibration knob Press the Check Position button to verify the adjustment The picker spotter head moves to the home position and then back to its new XY position If necessary re calibrate the XY position Adjust the Z position so that a strip of thin aluminum foil is barely stuck between the needle and the knob Do as follows 1 Carefully lower the needle 0 1 mm steps while moving slightly the aluminum foil sideways Stop lowering when the foil is stuck 2 Press the Check Position button to verify the adjustment The foil should stick 3 Raise the needle 0 1 mm and press the Check Position button to verify The aluminum foil should not stick 4 Lower the needle 0 1 mm and press the Check Position button to verify that the aluminum foil now sticks Re calibrate if necessary Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 e Check that the tip of the needle points precisely to the center of the calibration knob see figure above item 4 6 1 9 e If necessary re calibrate the XY position 11 Click on Next 7 Digester Needle Change Ettan Workstation Service Program Amersham Biosciences x 12 Click on Finish to save the changed position values 13 Remove the calibration plate Ettan Spot Handling
7. 4 User Maintenance 76 The picker head moves to the calibration position 10 Adjust the XYZ position of the picker head against the calibration knob using the Nudge Picker Head position buttons 11 Press the Check Position button to verify the adjustment The picker head moves to the home position and then back to its new XYZposition 12 Click on Next 13 Click on Finish to save the changed position 14 Remove the calibration plate Proceed by calibrating the distance between the picker head and the camera For instructions on camera alignment see Section 4 5 IMPORTANT The new picker head must be registered in the Picker Head Management see Ettan Spot Handling Workstation User Manual Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 4 9 Priming Priming the syringes removes air bubbles in the spot picker spotter and digester tubing and also rinses the tubing with fresh solution Priming is performed as follows e before starting spot picking spotting and digestion processing see descriptions 4 9 1 4 9 2 4 9 3 and 4 9 4 below e automatically during spotting and digestion processing However additional priming should be performed e after replacement of syringes e if the system will not be used for a longer period of time and is to be filled with distilled water e if the system is contaminated Note After priming check that all tubing outside
8. Denmark Tel 45 70 25 24 50 Fax 45 45 16 2424 e Eire Tel 1 800 709992 Fax 44 1494 542010 e Finland amp Baltics Tel 358 9 512 3940 Fax 358 9 512 39439 e France Tel 01 69 35 67 00 Fax 01 69 41 98 77 e Germany Tel 0800 9080 711 Fax 0800 9080 712 e Greater China Tel 852 2100 6300 Fax 852 2100 6338 e Italy Tel 02 26001 320 Fax 02 26001 399 Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 Korea Tel 82 2 6201 3700 Fax 82 2 6201 3803 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 e Middle East amp Africa Tel 30 210 96 00 687 Fax 30 210 96 00 693 Netherlands Tel 0800 82 82 82 1 Fax 0800 82 82 82 4 e Norway Tel 47 815 65 777 Fax 47 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C I S amp N I S Tel 7 495 956 5177 Fax 7 495 956 5176 e Spain Tel 902 11 72 65 Fax 935 94 49 65 e Sweden Tel 018 612 1900 Fax 018 612 1910 e Switzerland Tel 0848 8028 10 Fax 0848 8028 11 e UK Tel 0800 515 313 Fax 0800 616 927 USA Tel 1 800 526 3593 Fax 1 877 295 8102 imagination at work 18 1153 54 AD 08 2006
9. Reagent bottles for the Spot Picker amp Spotter Waste bottles Fig 2 2 Reagent waste bottles and syringe pump module Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 2 The Cabinet The purpose of the cabinet is to house all mechanics for operator and personnel safety to protect personnel from hazardous chemicals and to avoid contamination of samples in process The operator has access to all components via the cabinet doors The cabinet consists of two main parts 1 The left part contains see Fig 2 3 e Digester and Spot Picker amp Spotter upper compartment e computer and temperature O controller lower compartment 2 The right part contains see Fig 2 4 e robot dryer incubator and hotel for gel trays microplates and MS sample trays upper compartment e power supply and control equipment for the robot placed on a sliding shelf in the lower compartment The cabinet is equipped with an evacuation fan for transporting process air to the ventilation system Since most of the evacuated air will contain fumes that are heavier than air the inlet to the evacuation fan is located at a low level In addition the cabinet has filters for the incoming air to minimize contaminants in the cabinet Temperature 1 0 controller Computer Fig 2 3 The cabinet s lower left compartment Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 19 2 System d
10. System Manual 18 1153 54 Edition AD Introduction 1 1 Introduction IMPORTANT Ettan Spot Handling Workstation is intended for research use only and should not be used in any clinical procedures for diagnostic purposes Ettan Spot Handling Workstation is a fully integrated instrument for automatic processing and transfer of biomolecules from polyacrylamide electrophoresis gels to targets used in MALDI ToF mass spectrometer MS Processing is done in three steps 1 Spot picking from 2 D electrophoresis gels This step is defined as removing material of interest from polyacrylamide electrophoresis gels contained in gel plugs and dispensing it into microplate wells 2 Digestion of the picked proteins This step is defined as pre treatment washing destaining etc enzymatic cleaving and extracting biomolecules from the gel plugs Digestion comprises a number of substeps including dispensing into microplate wells and drying both as an intermediate and a last step 3 Spotting dispensing of samples onto targets for the MS This step is defined as pre treatment dissolving etc and transfer of extracted biomolecules and matrix solution from microplate wells or containers to MALDI targets 1 1 The System Manual This manual provides technical information process descriptions and start up shutdown instructions for Ettan Spot Handling Workstation In addition maintenance schedules and instructions for user m
11. a warning appears in the Handle Events dialog To acknowledge the warning select the warnings row and click Clear warning The error indicator will stop flashing Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 21 2 System description Power indicator blue Running indicator blue Error indicator red Emergency Stop push button red Reset push button yellow Fig 2 5 Front panel controls and indicators 22 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 2 2 External controls and connections The controls and connections located at the right side of the cabinet are shown in Fig 2 6 Mains power switch Mains power inlet Ventilation outlet Fig 2 6 Mains connections and ventilation outlet The following connections are located at the left side of the cabinet see Fig 2 2 1 Reagent bottle tubing 2 Waste bottle tubing 3 Syringe pump module tubing 2 2 3 Safety switches The upper doors of the cabinet are equipped with safety switches The switches are mounted at the same level as the lower part of the doors Cabinet door Safety switch Fig 2 7 Safety switch on the cabinet door Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 23 2 System description Video camera Y arm Spot Picker head Gel tray 24 2 3 The Spot Picker amp Spotter The Spot Picker amp Spotter is a robotic syste
12. and inside the workstation are filled with liquid and no air is visible Note Priming can also be done from the Batch Processing window refer to Ettan Spot Handling Workstation User Manual 4 9 1 Priming the Digester 1 Check that the tube fittings are properly tightened 2 From the menubar select System System Configuration 3 Click on System Service then click Start 4 Inthe Service Utilities dialog click on System Priming a System Priming Ettan Workstation Service Program Amersham Bio Estimated Duration Oh 7min Priming is not started Priming of digester is enabled Abort Priming of picker is enabled Priming of spotter is enabled Exit Digester Picker Spotter IV Enable Digester Priming Valve Positions to be primed Reservoir to valve priming volume 0 DH 5 ol Valve Position 1 Iv Valve Position 5 IV Valve to needle priming volume 0 50 5 ml Valve Position 2 IV Valve Position 6 I flow rate 1 16 87 16 87 ml min Valve Position 3 IV Valve Position 7 Valve Position 4 V Valve Position 8 I Valve Position for valve to needle priming 5 x Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 77 4 User Maintenance 78 The System Priming Digester dialog appears 5 Check the Enable Digester Priming box 6 Enter recommended values and check the Valve Position boxes for the valve positions which have connected liquids 7 Click on Start 8 The Digester moves to its rinse stati
13. follows e Hold the top of the picking piston with one hand and with the other unscrew the picker head from the base of the piston 74 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 e When the picker head is completely unscrewed from the base of the piston place it in a safe location until it is required once more Make sure that the retaining spring is also kept safe 8 Attach the new picker head as follows e Place the retaining spring over the screw thread on the top of the picker head e Screw the picker head onto the base of the piston making sure that the screw thread is inside the piston At the same time hold the top of the piston steady with your other hand e Slowly screw the picker head into the piston while holding the top of the piston still with the other hand Tighten the picker head against the stop position 4 Fig 4 5 Final appearance of the assembled picker head 9 In the Picker Head Change dialog click on Next i Picker Head Change Ettan Workstation Service Program Amersham bech oj x just XYZ position of picker head against calibration knob Press Check position to verify the adjustment Press Next when done fam at calibration position Nudge Picker Head position Y Zz a X 0 mm x ee Y 0 mm p Y Z 0 mm Check Position Ehe Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 75
14. incubator back to the Digester position in front of the enzyme container 12 The robot moves a new empty microplate from the hotel to the Digester position in front of the rinse station Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 13 The Digester moves the digested samples extracted from the gel to the empty microplate 14 The robot moves the finished microplate to the dryer for drying under a predetermined time 15 The robot moves the finished microplate to the Spot Picker amp Spotter 16 The robot moves an MS sample tray from the hotel to the position beside the microplate Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 43 2 System description 44 2 7 4 Spotting standard method 1 The Spotter dissolves the sample in the microplate by using Matrix solution 2 The Spotter mixes the sample in the microplate well by a defined number of aspirating dispensing cycles 3 The Spotter transfers the sample to the MALDI target 4 The sample is left on the MALDI target for air drying 5 The spotter needle moves to the rinse station for rinsing in spotter liquid The workstation continues the processing to complete the required batch run Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Start up and shutdown 3 3 Start up and shutdown This chapter provides start up and shutdown instructions for Ettan Spot
15. 0 mm above and 50 mm right of calibration position The digester needles move to a position 1 0 mm above and 50 mm to the right of the calibration position 7 Replace the digester needles as follows e Loosen and remove the retaining nuts with its tubing on top of the needle holder Tubing with tubing end ferrules Retaining nut Needle holder Digester needles Needle guide Note Be careful not to lose the tubing end ferrules e Remove the worn needles Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 69 4 User Maintenance e Carefully insert the new needles e Let the needles drop down through the holes in the needle guide e Attach the retaining nuts with its tubing e Finger tighten the retaining nuts e Loosen the stop screw with an Allen key Stop screw Allen key 8 Click on Next 9 Check that the needles are flush with the calibration plate i e the needles should be resting on the calibration plate Adjust if necessary 10 Tighten the stop screws while holding the needles slightly against the calibration plate 70 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 11 Check that the needles are flush with the calibration plate If not repeat the procedure 12 Click on Next U Digester Needle Change Ettan Workstation Service Program Amersham Bioscien xj just XY position of needles against calibration plate Press Ch
16. 02 76 10 x 1152 samples 4 HCCA 10 x 20 mg pre weighed for 10 full 17 6002 77 batch runs 10 x 1152 samples ImageMaster 2D Elite Software 80 6350 56 ImageScanner 18 1134 45 ImageScanner Geometry Calibration Kit 18 1145 55 Typhoon 8600 Ettan MALDI ToF Pro 120 V 18 1156 54 Ettan MALDI ToF Pro 220 V 18 1156 53 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 89 6 Specifications 90 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Index Index A ele associated documentation attaching DICK VCCI PE EE EE se E Ne 75 B backup r sults And data regeert 49 bareode E 37 Tel E sansene erd Aad 46 C ENE 19 calibration EE 63 fee MEIER 27 ele Ke Us wa 53 changing GIGESLERINCCCICS ee 68 picker hedd E spotter needle Elle cleaning FOU entree 52 COM PUST narrer 19 CONSUMA DIES nunnana r e E R es 45 D elle ER digester needles CNANGING aa 68 AE 31 E ENZUME COOL eebe eier 29 30 El Reegele 23 F PANE pinsin EE isla 19 Me 19 TROMMEZG ROD DSM CONG LA NE ROR 27 H MAN 36 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 91 Index 92 I O controller ceseccsssscsssssssssscsssssssssssssssssnssvesssuessesessesssssesesseeessstssessssssssssnsnsesssesnseeses 19 made SCANNET E 15 ion generator M maintenance reou sire en adsense 49 MALDI ToF MS target sv MATIX SOULOM Aanensen 24 microplate lid ENER Kea 33 O ordering information IC COSS O
17. 15 Multi Probe e CCD Camera K Door Interlock Multi Valve ed Run l LU Error I 2 1 stop Interlock GSIOC Enzyme Cooler Reset Barcode Robat Reader Mains cl Temp amp 1 0 Dryer Incubator Controller and Power Supply System for Robot Computer e Ethernet ooo Barcode Reader oll Mouse CI Fig 1 2 Ettan Spot Handling Workstation configuration The sub systems are described in detail in the System description chapter of this manual where brief descriptions of the individual components are also given Ettan Spot Handling Workstation control software is described in the separate Ettan Spot Handling Workstation User Manual and Online Help The package contents are described in Section 1 3 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Introduction 1 1 2 1 Rating label The rating label is located at the right side of cabinet close to the mains connections The layout of rating label see figure below Ettan Spot Handling Workstation Voltage 100 120 230 240 V Frequency 50 60 Hz Power 1700 VA Code no 18 1149 68 Made in Sweden L Fig 1 3 Rating label layout Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 13 1 14 Introduction 1 3 Package contents Ettan Spot Handling Workstation is delivered with the products listed in Table 1 1 F
18. Decon Laboratories Ltd Milli Q is a trademark of Millipore Corporation Voyager is a trademark of Applied Biosystems Inc All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information 2006 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric Company GE Healthcare Bio Sciences AB Bjorkgatan 30 SE 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 85 65 62751830 Fax 85 65 62751829 Australasia Tel 61 2 8820 8299 Fax 61 2 8820 8200 e Austria Tel 01 57606 1613 Fax 01 57606 1614 e Belgium Tel 0800 73 890 Fax 02 416 8206 e Canada Tel 1 800 463 5800 Fax 1 800 567 1008 e Central East amp South East Europe Tel 43 1 972 720 Fax 43 1 972 722 750 e
19. GE Healthcare Ettan Spot Handling Workstation System Manual Ettan Spot Handling Workstation 2 1 Ettan Important user information All users must read this entire manual to fully under stand the safe use of Ettan Spot Handling Workstation WARNING The WARNING sign highlights instructions that must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood WARNING Avoid exposure to hazardous laser radiation CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symbol indicates that the waste o
20. Handling Workstation Refer to Ettan Spot Handling Workstation User Manual for instructions on the consumables needed for a batch run 3 1 Starting the workstation To start Ettan Spot Handling Workstation 1 Turn on mains power to Ettan Spot Handling Workstation with the mains power switch at the right side of the cabinet see Fig 2 6 2 Check that Ettan Spot Handling Workstation is operating e the Power indicator should light e the PC should show the log on screen Power indicator blue Running indicator blue Error indicator red Emergency Stop push button red Reset push button yellow Fig 3 1 Ettan Spot Handling Workstation front panel 3 Log in to Windows 2000 Further operating instructions are provided in Ettan Spot Handling Workstation User Manual Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 45 3 Start up and shutdown 46 3 2 Before batch start up Before starting a batch run the following checks should be done CAUTION Always make sure that no foreign objects are left inside the cabinet 1 Check that the correct spot picker head for the actual batch run is selected For instructions on changing spot picker head see Chapter 4 User Maintenance of this manual 2 Check that there is not any calibration plate in the Spot Picker amp Spotter and the Digester 3 Check that there is not any microplate in the Spot Picker amp Spotter the Digester the inc
21. MICS vanskene nedersta related products P package contents performance specification picker head attaching changing priming ege lee 77 R reagent bottles m m 17 lee tee e E 89 TODO ae Ee ee 35 eege el EE 19 POWEF SUPO EE 19 S schedule user maintenance SERVO OND DEN eier SECTIOUN Eeer spot picker spotter spotter needle CNOMOING E 60 starting the workstation S SUD SYSTEMS siele eege SRIEIDIEIE DUN Pe user ates breed i system description Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Index T technical specification temperature controller EHS EE U eg alte le 51 V VIA 10010 0 o E 27 W waste bottles oooceecceceeessssssssssssssssseeescssssusseesssssseesssssssesessssssscecsssssessssssseessessssseseeessuness 17 A OTR OW ee 38 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 93 Index 94 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD www gehealthcare com GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden I Elanders sterv la 2006 Ettan ImageMaster Immobiline ImageScanner and Typhoon are trademarks of GE Healthcare companies GE imagination at work and GE monogram are trademarks of General Electric Company Microsoft and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries Decon is a trademark of
22. Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 10 Repeat steps 4 to 8 until all status bars are green Workstation id FS 2 Software version 21 SpotPicker Digester I Spotter System Service Press save to commit the alignment or close to abort Alignment status Not aligned Iterations 1 2 3 Confirm that the marker is placed on the pick head Start Save 11 Click the Save button to save the alignment values 12 Remove the gel tray 13 Close the cabinet door 14 Press Reset on the cabinet s front panel Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 59 4 User maintenance 4 6 Changing the spotter needle WARNING MOVING PARTS Do not read any barcodes with the external Ar barcode reader while performing user maintenance inside the cabinet Output signals from the barcode reader will be interpreted by the Spot Picker amp Spotter and Digester units as a command to move to their home positions Note Calibration plate code no 18 1156 66 is needed for the changing procedure Digester needles knobs 8 pos Picker head knob 1 pos Spotter needle knob 1 pos I Fig 4 3 Calibration plate 18 1156 66 60 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Gi Ettan Spot Handling Workstation 4 6 1 Changing procedure User Maintenance 4 1 From the menuba
23. Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 e Lift out the needle with its tubing and connector from the needle holder see figure below Connector Spotter needle Low retaining nut e Loosen the lower retaining nut Use a wrench to hold the connector while loosen the nut Do not remove the retaining nut e Remove the worn needle e Carefully insert the new needle Press the needle against its innermost position e Fingertighten the retaining nut e Carefully insert the needle with its tubing and connector into the needle holder e Tighten the four screws while holding the tip of the needle approx 1mm above the calibration plate Be carefully not to scratch the needle against the calibration plate Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 65 4 User Maintenance 66 9 Click on Next Di Spotter Needle Change Ettan Workstation Service Program Amersham Bii djust XYZ position of needle against calibration knob Press Check position to verify the adjustment Press Next when done Arm at calibration position Nudge Needle position Pimm 8 I Orm a ETT I Z Omm 21 Check Position The spotter needle moves to the calibration position 10 Adjust the XYZ positions against the calibration plate using the Nudge Needle position buttons as follows Carefully lower the needle towards the calibration knob Stop lowering when the needle is approx
24. Workstation System Manual 18 1153 54 Edition AD 67 4 User Maintenance 4 7 Changing the digester needles WARNING MOVING PARTS Do not read any barcodes with the external Ar barcode reader while performing user maintenance inside the cabinet Output signals from the barcode reader will be interpreted by the Spot Picker amp Spotter and Digester units as a command to move to their home positions Note Calibration plate code no 18 1156 66 is needed for the changing procedure see Fig 4 3 4 7 1 Changing procedure 1 From the menubar select System System Configuration 2 Click on System Service then click Start 3 In the Service Utilities dialog click on Change Digester Needles Li Calibration knobs for digester needles 4 Open the cabinet door directly after opening the cabinet door s since the digester head can make sudden rapid movements Wait at least 30 seconds before entering this working area 1 WARNING MOVING PARTS Do not enter the Digester s working area 5 Place the calibration plate onto plate position 1 68 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 6 Inthe Digester Needle Change dialog click on Next eh Digester Needle Change Ettan Workstation Service Program Amersham Biosciences E emove the old needles Fit the new needles Connect the tubing to the needles Loosen the stop screws ress Next when ready Arn 1
25. aintenance are included Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 9 1 10 Introduction 1 2 System overview Ettan Spot Handling Workstation consists of the following sub systems see Fig 1 1 Cabinet Spot Picker amp Spotter Digester with dryer and incubator Transfer robot Gel hotel Microplate MS sample tray hotel Ettan Spot Handling Workstation control software running under Windows 2000 operating system on a PC PC with frame grabber card for video display Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Introduction 1 Transfer robot Dryer hidden Digester Incubator hidden Reagent bottles Waste bottles Cabinet Gel hotel Spot Picker amp Spotter Microplate MS sample tray hotel Fig 1 1 Ettan Spot Handling Workstation Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 11 1 12 Introduction Control Panel Ventilation Fan Syringe Pump Gilson 215 Camerata SRAGGER Gilson 2
26. eck position to verify the adjustments Press ext when done fm 1 0 mm above calibration position Nudge Needle position Y a X 0 mm Xx 441 Y 0mm v The digester needles move to a position 1 0 mm above the calibration position 13 Adjust the XY position of the needles against the calibration plate using the Nudge Needle position buttons The tip of the needles must be clearly within the area of the calibration knobs 14 Press the Check Position button to verify the adjustments The digester needles move to the home position and then back to the new XY position 15 Visually check the needle positions If necessary re calibrate XY positions 16 Click on Next 7 Digester Needle Change Ettan Workstation Service Program Amersham Bioscienct x i values have been saved Remove calibration unit from plate position one Position not changed Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 71 4 User Maintenance 17 Click on Finish to save the changed position 18 Remove the calibration plate 72 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 4 8 Changing picker head barcode reader while performing user maintenance inside the cabinet Output signals from the barcode reader will be interpreted by the Spot Picker amp Spotter and Digester units as a command to move to their home positions 1 WARNING MOVING PARTS Do not read any barcodes
27. er 2 From the menubar select System System Configuration mm E Reagent Catalog System Configuration Method Management Batch Configuration Batch Processing User Total Max spots 152 Administrator lt System Configuration Workstation id seuppw000086 Software version 2 1 Digester I Spotter Camera Alignment System Serice Close Volume Flow rates Rinse 0 5 ml Aspiration I 20 ml min Dispensing 20 ml min Rinse 30 ml min Connected liquid Z offsets Picking 0 2 mm Dispensing sa mm Picker head System prime Currently active 001 2 0 mm EI Volume 10 mi Jazz amplitude glass 1 2 mm Flow rate 30 ml min Jazz amplitude plastic 1 1 mm Camera aligned Yes 2 26 03 3 Click on the Camera Alignment tab WARNING MOVING PARTS Do not enter the Spot Picker amp Spotter s working area directly after clicking the camera alignment tab since the picker spotter head and camera assembly can make sudden rapid movements Wait at least 30 seconds before applying the camera alignment foot Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 4 Apply the calibration alignment foot onto the picker head 5 Close the cabinet door 6 Press Reset on the cabinet s front panel 7 Check the Confirm that the marker is placed on the pick head box I System Configuration
28. escription The robot s power supply The robot s control equipment Fig 2 4 The cabinet s lower right compartment 20 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 2 1 Front panel controls and indicators The layout of the Ettan Spot Handling Workstation front panel is shown in Fig 2 5 The front panel has two control buttons Emergency Stop When activating this red push button the workstation s instruments and the transfer robot immediately cease movement and all motors are de energized WARNING If the Picker Spotter or the Digester is in its homing procedure Ar when the emergency stop button is activated the homing will be completed before the instrument ceases movement Homing means that the unit is seeking its zero position The text HOMING is shown in the unit s display Reset This non latching push button is used to acknowledge closed cabinet doors The push button s yellow indicator is on when one or several cabinet doors are opened and will go off after acknowledgement Power This blue indicator is on when the mains power is switched on i e when power is present in the workstation Running This blue indicator is on during batch processing and is flashing when a batch process is in Pause Batch mode The indicator is off when no batch is present in the workstation Error This red indicator is on if an error occurs and is flashing when
29. f electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Contents Contents LIST OT TIO TE 7 1 Introduction 1 1 The System MOU oasen 9 1 2 System OVEFVIOW siiin E 1 2 1 Rating label 1 3 Package contents L ACCESSO S EAR 1 5 Associated documentotion srst tsntrstsnrs nrn nenea 15 1 5 1 Ettan Spot Handling Workstation Safety Handbook 15 1 5 2 Ettan Spot Handling Workstation Site Preparation Guide 15 1 5 3 Ettan Spot Handling Workstation User Manual 15 1 5 4 Ettan Spot Handling Workstation Online Help 15 2 System description QM EE 17 22 TINS CDI St ass eegiieiereee see 19 2 2 1 Front panel controls and indicators esmmm 21 2 2 2 External controls and connections s msmmmmmmsmssmms 23 223 e EE 23 2 3 The Spot Picker amp Gpotter 24 2 3 1 The Picker hedd neesessessevvsssseerrrsseesssssssserersssesssssssssrressner 26 2 3 2 Video comerg EE 27 2 3 3 THE SPOtter NeddLunuansonsenemenesnnenssruenvanante 28 2 4 The digestion SYSteNM ss
30. g Workstation System Manual 18 1153 54 Edition AD 29 2 System description 2 4 1 The multiple probe head The 8 needle multiple probe head is mounted on the Z arm Z arm Multiple probe head Digester needles Fig 2 16 The 8 needle multiple probe head Enzyme container Enzyme cooler Fig 2 17 The enzyme cooler 30 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 4 2 The dryer The purpose of the dryer is to evaporate the liquid gel plug in the microplate wells and to dry peptide extract The dryer consists of open chambers with space for six microplates Each microplate position has a heater located underneath The dryer is also equipped with fans for air circulation An air distributor is placed above the microplate position to direct the air flow into each microplate well An evacuation fan in the cabinet then transports the air to the ventilation system Note The upper two microplate positions have no air circulation Microplate position Heater Air distributor Fig 2 18 The dryer Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 31 2 System description 2 4 3 The ion generator The purpose of the ion generator is to neutralize the electrostatic charged air surrounding the microplates when placed in the dryer and thus minimize the electrostatic influence on the gel plugs The ion generator is mounted in a separate box attached t
31. head moves to the rinse station for washing using Milli Q water optional Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 39 2 System description 2 7 3 Digestion 1 The robot moves the finished microplate with gel plugs and picker liquid up to the Digester 2 The Digester aspirates the picker liquid from the gel plug wells and moves it to the rinse station 3 The Digester destains and equilibrates the gel plugs remaining prior to enzyme addition and digestion by one or several washing procedures 4 The robot moves the finished microplate to the dryer for dehydration of the gel plugs under a predetermined time to enhance enzyme entry into the gel plug before digestion 5 The robot moves the microplate back to the Digester 40 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 6 The multi probe head moves to the enzyme container 7 The Digester adds enzyme e g Trypsin which enters into and rehydrates the gel plugs 8 The Digester adds buffer if needed 9 The multiple probe head moves to the rinse station Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 41 2 System description 42 10 The robot moves the finished microplate to the incubator for enzymatic digestion under a predetermined time During incubation the microplate is covered with a lid to avoid evaporation 11 The robot moves the microplate from the
32. ig 4 2 Accessories for camera alignment 4 5 2 Focus and aperture adjustment Before starting the camera alignment check that the camera lens is focused on the bottom of the gel tray Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 53 4 User Maintenance 1 From the menubar select System System Configuration ET Ettan Spot Handling Workstation File System BatchRun Reports Window Help mm E System Configuration Reagent Catalog workstation id seuppw000086 Software version 2 1 System mm Baot Picker 3 Digester Spotter Camera Alignment I System Service Close Configuration 2 e gt Method Management volume EEN Flow rates EE Rinse 0 5 mi Aspiration 20 ml min Dispensing 20 ml min Be Rinse 30 mifmin Configuration ss Batch Processing Connected liquid Z offsets Picking oa mm Dispensing 5 0 mm green Picker head System prime User Administrator ee yxA Currently active 001 2 0 mm a Volume 10 ml fer Jazz amplitude glass 1 2 mm Flow rate 39 ml min Max spots i ei Jazz amplitude plastic 1 1 mm Camera aligned Yes 2 26 03 Total 2 Click on System Service 3 Click on Start The Service Utilities dialog appears i Service utilities E View Camera Image Change Syringes Change Picker Head Change Spotter Needle Change Digester Needles Exit 54 Ettan Spo
33. iming is finished click on Exit 4 9 4 System priming Priming of the Digester the Picker and the Spotter can also be done at the same time Do as follows 1 Check the Enable Digester Priming the Enable Picker Priming and the Enable Spotter Priming boxes 2 Enter recommended values 3 Click on Start 4 When priming is finished click on Exit Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 79 4 User Maintenance 80 4 10 Changing syringes WARNING MOVING PARTS Do not read any barcodes with the external barcode reader while performing user maintenance inside the cabinet Output signals from the barcode reader will be interpreted by the Spot Picker amp Spotter and Digester units as a command to move to their home positions 1 From the menubar select System System Configuration 2 Click on System Service then click Start 3 Inthe Service Utilities dialog click on Change Syringes U ServicePgmProSpot Syringe Change Amersham Biosciences ES Pressing any of the lower syringe buttons will move needle s picker head to it s wash station home pump and then lower corresponding isyringe s Lower Picker Syringe Exit 4 To enable replacement of syringels click on Lower Digester Syringes Lower Picker Syringe or Lower Spotter Syringe respectively The following will happen e The needle needles picker head movels to its rinse station e The syringe pump moves to its h
34. in the PC before displaying the image in Ettan Spot Handling Workstation control software Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 27 2 System description 2 3 3 The spotter head The spotter head is mounted on a bracket on the same Z arm as the spot picker head Spotter head Spotter needle Spot Picker head Fig 2 13 Spot picker and spotter heads Spotter needle Rinse station MALDI target MS sample tray 96 well microplate Vial holder Fig 2 14 Spotting onto a MALDI target 28 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 4 The digestion system The Digester is a robotic system designed to prepare gel plugs for enzymatic digestion of proteins and subsequent extraction of peptides The instrument has an 8 needle multiple probe head that can move in the X Y and Z directions The multiple probe head is connected to an 8 channel multi probe dilutor An 8 position valve actuator with a common outlet is used to select the different liquid solvents The rinse station has two positions for rinsing one for shallow rinsing of the needles and one for deep rinsing Also included in the digestion system are a dryer and an incubator see descriptions below Valve actuator Rinse station Multi probe dilutor Multiple probe head Enzyme cooler Enzyme container Microplate Fig 2 15 The Digester Ettan Spot Handlin
35. ition AD 25 2 System description 26 2 3 1 The picker head The picker head is mounted on the Z arm just behind the video camera Tube fitting Piston Retaining spring Punch Fig 2 10 The picker head The retaining spring creates a pressure against the gel backing during picking thus ensuring that the gel plug will be properly excised When the Z height is correctly adjusted the picker head should be lowered until a gap is formed between the piston and the body of the picker head thereby allowing the spring to press against the gel backing Fig 2 11 Spring action when lowering the picker head Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 3 2 Video camera The video camera is used to establish the coordinates for the gel to be picked By detecting the reference markers on the gel and comparing with the coordinates in the pick list the picking coordinates can be determined Since the camera lens is located at a distance from the picker head its position relative to the picker head has to be precisely defined to ensure high precision picking The camera alignment routine provides the means for this see Chapter 4 Also instructions for adjustment of camera focus and aperture are provided in Chapter 4 Aperture setting Focus setting Fig 2 12 The video camera The video signal is processed by a frame grabber card
36. ling Workstation 1 5 3 Ettan Spot Handling Workstation User Manual Contains detailed operating instructions 1 5 4 Ettan Spot Handling Workstation Online Help An online help function can be reached from the software A troubleshooting guide is also included in this function Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 15 1 Introduction 16 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 System description 2 1 General Fig 2 1 Ettan Spot Handling Workstation Ettan Spot Handling Workstation consists of fully integrated instruments for the automatic processing and transfer of biomolecules from polyacrylamide electrophoresis gels to MALDI ToF MS targets To control the system a personal computer running Ettan Spot Handling Workstation control software is included Communication between the computer and the various modules and components of Ettan Spot Handling Workstation is achieved via high speed data network connections Reagent bottles for the fluid handling equipment and the syringe pump module are placed on shelves to the left of the cabinet The waste bottles are placed on the bottom shelf The bottles are connected to the instruments via tubing through the cabinet wall see Fig 2 2 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 17 2 System description 18 Reagent bottles for the Digester Syringe pump module
37. m that can move the picker spotter head in three directions X Y and Z The syringe pump see Fig 2 9 allows precise handling of liquid and picked gel plugs The valve next to the syringe pump controls the flow from the liquid supply and to from the picker spotter head The picker head and the spotter head are mounted on the same robot arm This is possible since they are not used at the same time The picker head movements are guided by a camera system The camera which is attached to the Z arm is connected to the PC via a frame grabber card Reference markers on the gel are identified using the camera This enables the pick list coordinates from the scanned gel to be transferred to picker head positions during automated picking Two microplate supports and a vial holder for matrix solutions and calibrants are placed beside the gel tray Z arm X arm Rinse station for Waste tube Rinse station Spot Picker for Spotter Spotter head Gel tray support 4 pcs Microplate support 2 pcs Vial holder Fig 2 8 The Spot Picker amp Spotter Note Fig 2 8 shows a previous spotter head design Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 Valve Syringe pump for Spot Picker Syringe pump for Spotter Fig 2 9 The Syringe pump module The syringe pump module is placed on a shelf to the left of the cabinet see Fig 2 2 Ettan Spot Handling Workstation System Manual 18 1153 54 Ed
38. nual 18 1153 54 Edition AD 85 6 Specifications 6 2 Performance specification Table 6 2 Ettan Spot Handling Workstation performance specification Ettan Spot Handling Workstation Process capacity 12 Ettan DALT II gels and 1152 protein spots per batch in approx 30 hours depending on method 6 3 Regulatory requirements Ettan Spot Handling Workstation meets or exceeds the standards listed in Table 6 3 Table 6 3 Regulatory requirements Ettan Spot Handling Workstation Safety UL 1260 UL 1492 camera CSA C22 2 No 151 IEC 1010 1 EMC EN 50082 1 EN 50022 frame grabber FCC Class A EMI EN 50081 1 EN 50024 frame grabber 86 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Specifications 6 6 4 Ordering information 6 4 1 Accessories Table 6 4 lists the accessories that can be ordered from GE Healthcare Table 6 4 Accessories for Ettan Spot Handling Workstation Product Quantity Code No Ettan Spot Handling Workstation Ettan Microplate 96 well 100 pcs 1 18 1150 42 Reagent bottle 1 18 1158 79 Waste bottle 1 18 1158 81 Sealing for incubator lid 12 pcs 1 18 1160 40 Gel tray trolley 1 18 1149 79 Spot Picker Picker head 1 4 mm for 1 0 mm thick gels 1 18 1147 03 Picker head 2 0 mm for 1 0 mm thick gels il 18 1143 64 Reference markers sheet incl 560 pcs 2 18 1143 34 Gel holder 2 pcs 1 8 1156 27 Gel tray incl gel holders and glass lid 1 8 1150 39 Glass lid for gel tra
39. o the upper part of the dryer see figure below lon generator box Dryer Fig 2 19 The ion generator 32 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 4 4 The incubator The purpose of the incubator is to establish a constant temperature during enzymatic digestion at a predefined time The incubator has one closed chamber with space for twelve microplates and is equipped with heating control During incubation the microplates are covered with separate lids see Fig 2 21 to avoid evaporation The lid is located above each microplate position and is placed onto the microplate by the robot when it inserts the microplate into the incubator Incubator Microplate lid 12 pcs Fig 2 20 The incubator Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 33 2 System description Fig 2 21 Microplate lid 34 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 5 The transfer robot A robot system with six degrees of freedom is used as a bridge between the different modules in Ettan Spot Handling Workstation The robot handles gel trays microplates and MS sample trays as well as opens and closes the incubator door In addition the robot can handle the lids for both gel trays and microplates The robot is equipped with a servo gripper for handling gel trays as well as microplates and MS sample trays Servo gri
40. ome position e The syringes lowers lower Note When replacing the spotter syringe remember to mount the enclosed sealing into the connection between the tubing and the new syringe 5 Before replacement the plunger of the new syringe should be wetted as Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 follows e Remove the plunger from the barrel e Dip the plunger tip into Milli Q water and re insert the plunger into the glass barrel e Work the plunger up and down 5 6 times re wetting the tip if necessary 6 After replacement of syringe s click on Exit Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 81 4 User Maintenance 82 ped 4 11 Preventive maintenance GE Healthcare recommends preventive maintenance PM to be performed by qualified service personnel during the yearly service visit The extent of the PM is to be agreed in the service contract Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Troubleshooting 5 5 Troubleshooting For information on troubleshooting refer to Ettan Spot Handling Workstation User Manual and Online Help
41. on 9 When priming is finished click on Exit 4 9 2 Priming the Picker 1 Check that the tube fittings are properly tightened 2 Inthe System Priming dialog select Picker 3 System Priming Ettan Workstation Service Program Amersham Biosci Estimated Duration Oh 7min or Start Priming is not started dd Priming of digester is enabled Abort Priming of picker is enabled Priming of spotter is enabled Exit IV Enable Picker Priming Priming volume 0 100 10 ml flow rate 1 30 30 ml min 3 Check the Enable Picker Priming box A Enter recommended values 5 Click on Start 6 The Spot Picker amp Spotter moves to its rinse station 7 When priming is finished click on Exit 4 9 3 Priming the Spotter 1 Check that the tube fittings are properly tightened Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 2 In the System Priming dialog select Spotter fi System Priming Ettan Workstation Service Program Amersham Biosciene es Estimated Duration Oh 7min SE Start Priming is not started Priming of digester is enabled Abort Priming of picker is enabled Priming of spotter is enabled E AA IV Enable Spotter Priming Priming volume 0 10 3 ml flow rate 0 1 3 3 ml min 3 Check the Enable Spotter Priming box 4 Enter recommended values 5 Click on Start 6 The Spot Picker amp Spotter moves to its rinse station 7 When pr
42. or ordering information refer to Section 6 4 Table 1 1 Ettan Spot Handling Workstation delivered products Product Quantity Ettan Spot Handling Workstation control software Picker head 1 4 mm for 1 0 mm thick gels Picker head 2 0 mm for 1 0 mm thick gels N N N KH Reference markers sheet incl 560 pieces Gel tray 2 Gel holder 24 Glass lid for gel tray 2 Tubing Spot Picker amp Spotter Tubing Digester Tubing waste Camera calibration foot 5 Calibration plate Waste bottle 15 liter Reagent bottle 5 liter Reagent bottle 2 liter FP UN Q N Reagent bottle 1 liter Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Installation of AKTAcrossflow 10 1 4 Accessories In addition to the items delivered with the system Ettan Spot Handling Workstation requires the following accessories e Image scanner ImageScanner or Typhoon e Gel image evaluation software e g ImageMaster Nonlinear Dynamics See Chapter 6 for specifications and ordering information If you want to use other scanners please contact GE Healthcare 1 5 Associated documentation 1 5 1 Ettan Spot Handling Workstation Safety Handbook Provides the safety instructions that must be strictly followed for the safe use of Ettan Spot Handling Workstation 1 5 2 Ettan Spot Handling Workstation Site Preparation Guide Provides the data needed to prepare the laboratory for the installation of Ettan Spot Hand
43. partment ew wcswssss Fig 2 5 Front panel Controls and indicators msme Fig 2 6 Mains connections and ventilation outlet Fig 2 7 Safety switch on the Cabinet door Fig 2 8 The Spot Picker amp Spotter um Fig 2 9 The Syringe pump Module mums Fig 2 10 The picker head mu Fig 2 11 Spring action when lowering the picker head 26 Fig 2 12 The video Camera osm 27 Fig 2 13 Spot picker and spotter heads e eewmmmssmsmmsmsmm 28 Fig 2 14 Spotting onto a MALDI target Fig 2 15 The Digester eege Fig 2 16 The 8 needle multiple probe head mmse mmmmsmsmssmmsms 30 Fig 2 17 The enzyme cooler mmm 30 Fig 2 18 The dry r sssstascasccctiash aaaiti datas unten 31 Fig 2 19 The ion generator nmmnnersserersssssssrsrerssssssssesssenn 32 Fig 2 20 THE INCUDALOT enges eege 33 Fig 2 21 Fig 2 22 Fig 2 23 T Fig 2 24 Barcode reader mms 37 Fig 3 1 Ettan Spot Handling Workstation front panel ee 45 Fig 3 2 The Emergency Stop button on the cabinet s front panel 47 Fig 4 1 The System Configuration WINdOW ams 50 Fig 4 2 Accessories for camera olonment m mmseeemmsssieessessmessmssmss 53 Fig 4 3 Calibration plate 18 1156 66 nnmmmresssssssererrrrrsvsssssssrrrerersssssssssnnns 60 Fig 4 4 The picker head missen 73 Fig 4 5 Final appearance of the assembled picker head 75 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD List of figures 8 Ettan Spot Handling Workstation
44. pper Fig 2 22 The transfer robot Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 35 2 36 System description 2 6 The hotel The hotel is divided into two sections one containing shelves for microplates and MS sample trays and one with rails for gel trays MS sample tray Barcode reader Gel tray Microplate Microplate MS sample tray hotel Gel hotel Fig 2 23 The hotel 2 6 1 Gel hotel The gel hotel has space for twelve gel trays The bottom position of the hotel is used to lift off the glass lid from the gel tray When in this position the robot lowers the gel tray approx 10 mm and the glass lid remains on the shelves for temporary storage Note The gel tray has a silicone sealing on the top edge This protects the gel during storage 2 6 2 Microplate MS sample tray hotel The Microplate MS sample tray hotel can house 24 microplates and 12 MS sample trays for a batch run Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 2 6 3 Barcode reader DIRECTLY WITH OPTICAL INSTRUMENT CLASS 2 LASER PRODUCT Do not open cover to the internal barcode reader Refer servicing to qualified service personnel WARNING LASER RADIATION DO NOT STARE INTO BEAM OR VIEW The barcode reader located at the top of the hotel is used to track samples from gel to microplate to MALDI targets After moving a gel tray a microplate or a MS sample
45. r select System System Configuration File System BatchRun Reports Window Help Reagent Catalog System Configuration Method Management Batch Configuration Batch Processing User administrator Max spots 1152 Total System Configuration Workstation id Software version 2 1 seuppw000086 Close Ier Digester I Spotter Camera Alignment System Service Volume Flow rates Rinse 0 5 ml Aspiration 20 mimin Dispensing 20 ml min Rinse 30 ml min Connected liquid Z offsets Picking 0 2 mm Dispensing 5 0 mm Picker head System prime Currently active E Volume i 10 ml Jazz amplitude glass 2 Flow rate 30 ml min Jazz amplitude plastic LA mm Camera aligned Yes 2 26 03 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 61 4 User Maintenance ET Ettan Spot Handling Workstation File System BatchRun Reports Window Help 2 Click on System Service Bm E Reagent Catalog System Configuration Method Management Batch Configuration Batch Processing User administrator Max spots 1152 Total L a System Configuration Workstation id seuppw000086 Software version 2 1 Spot Picker I Digester I Spotter Camera Alignment Esme 7 Close Press the start button to launch the service utility Start 62 3 Click on Start Et
46. srin 29 2 4 1 The multiple probe beod 30 2 4 2 The dryer sms 2 4 3 The ion generator 244 TOSHIBA Kaasa 2 5 The transfer robot EE 35 eet 36 261 Gel MOLE E 36 2 6 2 Microplate MS sample tray hotel 36 263 BARCODE redder angrende ST 2 7 Workflow in Ettan Spot Handling Workstation 38 21 BOES ebe 38 2 7 2 Spot pekinese Eai 38 273 DES vu 40 2 7 4 Spotting standard method 44 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 5 Contents 3 Start up and shutdown 3 1 Starting the workstation eseseeeesssereesseesn 45 3 2 Before batch stort up 46 33 PUSS batch m ngen deiere 46 3 4 Shutting down the workstation ssmmsmssmesmsmmm 47 3 5 Emergency shutdown m m 47 3 5 1 Reset the Emergency StOP eeeecmsmsmswmsmsmssmssmmm 48 4 User Maintenance 4 1 System administration 4 2 User interface ssnnnnnnmeeeeessssserssereeeseen 4 3 User maintenance schedule sssssssssssssssssssssssssssersserevsserssereen 51 EI e elei ell PrOCEO UPS messsmensssssmssssssnssssisnsnnsss NNE 52 GE AS dE 52 442 GOL AOU S scessotsassssesceesstzceesios Eeer 52 443 McropldteldsLvuurvsteesaneuaseeeeer 52 Ab Camera alignment sssseeeememv 53 4 5 1 Required occeseories m m 53 4 5 2 Focus and aperture adjustment e 6H s emwwswsesswsmsmmmssme 53 4 5 3 Alignment procedure s s ss sssssnissssmmsssssisssmsssssmnssssssssss 55 4 6 Changing the spotter needle ee
47. ssing Connected liquid Z offsets Picking 0 2 mm Dispensing 5 0 mm Geesse Picker head System prime User Administrator EE VG Currently active 001 2 0 mm a Volume 10 ml Jazz amplitude glass 1 2 mm Flow rate 30 ml min Max spots Jazz amplitude plastic 1 1 mm Camera aligned Yes 2 26 03 Total Fig 4 1 The System Configuration window 50 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 4 3 User maintenance schedule Table 4 1 lists the maintenance operations that should be performed by the user at regular intervals Table 4 1 User maintenance Interval Action Instructions reference Daily Check the transfer robot s Due to the risk of collision working area check that no foreign objects are left in the cabinet When required Clean the workstation Wipe the cabinet and the instrument covers with a soft damp tissue Let dry completely before use See Section 4 4 Clean the microplate lids See Section 4 4 3 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 51 4 User Maintenance 52 4 4 Cleaning procedures 4 4 1 Instruments Cleaning the instruments regularly For proper function the instruments should be kept clean and dry Chemical stains and dust should be removed 1 Wipe the instrument covers with a soft damp tissue If needed use a mild detergent to remove stains 2 Let the instrument dry completely before use
48. t Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 4 Click on View Camera Image This will open a camera window on the screen 5 Place the gel tray onto its support in the Picker Spotter 6 Place a piece of white paper size approx 100 x 100 mm with printed black text into the corner of the tray beneath the camera Ensure that the paper lays flat on the bottom of the tray 7 If necessary adjust the aperture to normal brightness by using the aperture setting ring see figure below Aperture setting ring Focus setting ring 8 Adjust the focus to a sharp picture by using the focus setting ring see figure above 9 IMPORTANT Set the aperture to 4 four 10 Remove the gel tray 11 Perform the camera alignment procedure see Section 4 5 3 below 4 5 3 Alignment procedure CAUTION Before starting camera alignment make sure that the Zarm is properly mounted as high as possible on the Y arm 1 Prepare the gel tray as follows see Fig 4 2 e Place a piece of white paper in the center of the gel tray using adhesive tape Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 55 flettan Spot Handling Workstation File System BatchRun Reports Window Help 4 User Maintenance e Place a strip of double sided adhesive tape onto the white paper across the alignment position in the center of the gel tray e Place the gel tray onto the gel tray support in the Picker Spott
49. tan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 The Service Utilities dialog appears View Camera Image Change Syringes Change Picker Head Change Spotter Needle Change Digester Needles Exit A Inthe Service Utilities dialog click on Change Spotter Needle 5 Open the cabinet door WARNING MOVING PARTS Do not enter the Spot Picker amp Spotter s Ar working area directly after opening the cabinet door s since the picker spotter head can make sudden rapid movements Wait at least 30 seconds before entering this working area 6 Place the calibration plate onto plate position 1 TO amp Calibration knob for picker head A Oo Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 63 4 User Maintenance 7 Inthe Spotter Needle Change dialog click on Next Di Spotter Needle Change Ettan Workstation Service Program Amersham Biosciences E The spotter needle moves to a position 1 0 mm above and 50 mm to the right of the calibration position Note The Spotter Needle Change dialog shows a picture of the previous spotter head design 8 Replace the spotter needle as follows e Loosen the four needle holder screws with an Allen key see figure below Do not remove the needle holder Tubing Upper retaining nut Connector Lower retaining nut Needle holder Screw 4 pcs Spotter needle 64 Ettan Spot Handling
50. the Emergency Stop button or if a cabinet door is opened during a run the workstation s instruments and the transfer robot immediately cease movement and all motors are de energized when the emergency stop button is activated the homing will be completed before the instrument ceases movement Homing means that the unit is seeking its zero position The text HOMING is shown in the unit s display 1 WARNING If the Picker Spotter or the Digester is in its homing procedure e 9 9 Emergency Stop push button red Fig 3 2 The Emergency Stop button on the cabinet s front panel Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 47 3 Start up and shutdown 3 5 1 Reset the Emergency Stop IMPORTANT Do not switch off mains power When mains power is switched on after a switch off the transfer robot cannot perform homing Call GE Healthcare Service For information how to reset the emergency stop re start or abort a batch run refer to Ettan Spot Handling Workstation User Manual 48 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD User Maintenance 4 4 User Maintenance User maintenance is important for safe and trouble free operation of Ettan Spot Handling Workstation Preventive maintenance should be performed on a yearly basis by qualified service personnel This chapter provides instructions for user maintenance and service operations
51. tray from the hotel the transfer robot will stop at the reading position in front of the barcode reader Fig 2 24 Barcode reader Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 37 2 System description 38 2 7 Workflow in Ettan Spot Handling Workstation This section gives a brief step by step description of the workflow through Ettan Workstation For detailed description refer to Ettan Spot Handling Workstation User Manual 2 7 1 Batch run start 1 The workstation s control software starts to identify all gel trays microplates and MS sample trays 2 The transfer robot moves the gel tray to the bottom position of the hotel to lift off the glass lid 3 The robot moves the gel tray to the Spot Picker amp Spotter 4 The robot picks up a microplate from the hotel and moves it to the Spot Picker amp Spotter 2 7 2 Spot picking 1 The Spot Picker locates the reference markers 2 The picker head moves to the rinse station 3 The picker head moves to a gel spot according to pick list coordinates Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD System description 2 4 The gel spot is released from the gel backing under liquid by punching and sideways movements jazz 5 The gel plug is aspirated using Milli O water as hydraulic liquid 6 The gel plug is dispensed into a microplate well together with Milli Q water or destaining buffer 7 The picker
52. ubator and the dryer 4 Check that there is not any gel tray glass lid in the lower part of the gel hotel 5 Check that all microplates and MS sample trays are placed into the center position in the frame of the hotel shelf 6 Check that tubing and couplings are connected and tightened 7 Check that the waste bottles are empty Further operating instructions are provided in Ettan Spot Handling Workstation User Manual 3 3 Pause batch mode The pause batch mode can be used when a temporary stop is needed e g to remove a foreign object Proceed as follows 1 Inthe Batch Processing dialog click the Pause button 2 Wait until the Running indicator on the cabinet s front panel flashes 3 Open the cabinet door s and remove the foreign object 4 Close the cabinet door s 5 Dress the Reset button on the cabinet s front panel Further operating instructions are provided in Ettan Spot Handling Workstation User Manual Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD Start up and shutdown 3 3 4 Shutting down the workstation When not in use shut down the workstation 1 Inthe menubar choose command File Exit 2 Log out from Windows 2000 3 Switch off mains power with the mains power switch at the right side of the cabinet 3 5 Emergency shutdown In the event of an emergency press the Emergency Stop button on the front panel of Ettan Spot Handling Workstation see Fig 3 2 below When pressing
53. with the external The Spot Picker can be used with two sizes of picker heads 1 4 and 2 0 mm The picker heads are easily changed by a simple screw in screw out method After changing a picker head a camera alignment should be performed to establish the offset between the picker head and the camera lens The gel Z position may also have to be adjusted in the System Configuration Spot Picker window O ring Fig 4 4 The picker head Note Calibration plate code no 18 1156 66 is needed for the changing procedure see Fig 4 3 4 8 1 Changing procedure 1 From the menubar select System System Configuration 2 Click on System Service then click Start Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 73 4 User Maintenance 3 In the Service Utilities dialog click on Change Picker Head Calibration knob for picker head 4 Open the cabinet door working area directly after opening the cabinet door s since the picker spotter head can make sudden rapid movements Wait at least 30 seconds before entering this working area 1 WARNING MOVING PARTS Do not enter the Spot Picker amp Spotter s 5 Place the calibration plate onto plate position 1 6 Inthe Picker Head Change dialog click on Next Calibration knob for picker head d 210 The picker head moves to a position 100 0 mm to the left of the calibration position 7 Remove the picker head as
54. y 1 8 1156 28 Syringe 1 ml 1 8 1143 41 Tubing and fittings 1 8 1143 63 Inlet tubing package 1 8 1156 29 Camera alignment foot 5 8 1143 33 Spotter Syringe 100 ul 1 8 1143 55 Needle 1 8 1166 15 Tubing and fittings 1 8 1166 14 Vial with cap 1 ml 100 pcs 1 8 1152 75 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 87 6 Specifications Product Quantity Code No Ettan Sample Slide Kit 2 18 1147 18 MS sample tray Bruker Bruker Daltonics 18 1155 99 Micromass Micromass Ltd 18 1156 00 Voyager 18 1156 02 Digester Tubing and fittings 18 1156 31 Syringes 500 ul 8 18 1170 86 Needles 2 18 1143 57 Enzyme container 48 pcs 18 1157 75 Calibration plate 18 1156 66 Delivered without targets 88 Ettan Spot Handling Workstation System Manual 18 1153 54 Edition AD 6 4 2 Related products Specifications 6 Table 6 5 list products that are related to Ettan Spot Handling Workstation These products can be ordered from GE Healthcare Table 6 5 Products related to Ettan Spot Handling Workstation For code numbers marked with an asterisk see www gehealthcare com Product Code No Ettan DALT II large vertical gel system Ettan DALT II Pre cast Gel 12 5 17 6002 36 Ettan DALT II Buffer Kit 17 6002 50 Immobiline DryStrip gels and buffers Trypsin 10 x 250 ig for 10 full batch runs 17 6002 75 10 x 1152 samples TFA 50 ml for 10 full batch runs 17 60
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