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X-Cite ® exacte USER`S GUIDE

1. SJ T 11363 2006 SJ T11363 u d e LUMEN DYNAMICS Fluorescence Illumination In Control 14 Warranty Lumen Dynamics warrants the original purchaser for a period of one 1 full year calculated from the date of purchase that the equipment sold is free from
2. 22 6 8 Viewing Accumulated Lamp 22 6 9 Closed Loop Feedback M CLF eese nnn 23 7 Calibration Radiometry 24 7 2 X Cite exacte Power Reading using the XR2100 Power Meter or XR2000 24 73 X Cite exacte Calibration using the XR2100 XR2000 8 Light Guide 25 7 4 Calibrating X Cite exacte with the Objective Plane Power Sensor 750 26 N81 4 40 olio REDE 27 0 Warnings cua s cuo sor essa cea raa 28 MERCI Pin 29 9 External Control 30 91 Graphical User Interface 0 30 J2 WS OD 30 9 3 Installation from ZIP 30 94 Graphical User Interface 31 993 Icons MEE UU 32 Dal eee ee eee er 34 VOLO 34 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 20 gt D 34 9 9 Shutter Control via
3. 5 Figure 2 Rear Panel LUMEN DYNAMICS A Fluorescence Illumination In Control 5 Installation Set up 5 1 Installing Lamp Module 5 1 1 Be sure the AC POWER cord is disconnected from the unit 5 1 2 Remove the screw from the lamp housing side panel using the 3mm hex tool provided and remove the panel from the unit cover LAMP HOUSING SIDE PANEL Figure 3 Side Panel Tip Clips for holding the hex tool on the X Cite exacte are conveniently located underneath the system housing 5 1 3 Carefully remove the lamp module from its container holding only the ceramic component and lamp rim Caution The lamp module s operational life can be significantly shortened if handled incorrectly Be sure only to handle the ceramic surfaces and the lamp rim Do not touch the bulb s glass envelope or the inside surface of the reflector Skin oils can cause the lamp module to fail prematurely 5 1 4 As illustrated below position the lamp facing towards the front of the unit with the POWER leads facing towards you The lamp should be aligned so that the leading edge of the reflector lamp rim fits into the mounting groove on the lamp holder assembly LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control LAMP HOLDER LAMP RIM POWER CERAMIC LEADS COMPONENT Figure 4 Lamp Housing 5 1 5 Make sure the middle of the lamp
4. 35 9 10 Shutter control Via TTL eese 35 9 11 RS 232 Commands USB 36 9 12 X Cite 120PC Command ei 37 913 X Cite exacte Command 38 10 ERE 41 10 1 __4_ 6_6_6_____ _ _ _ 41 10 2 Power Up 42 Oo Camp ike FaU O mee ______ 42 104 LOW gt 43 10 5 ________ _ _ _ 6 43 10 6 LED Display i il de 43 10 7 Cooling Fan 43 11 Care and Maintenance tub ds Qus amc utu 44 44 11 2 UHN GUIO eee ee ee ee 45 AS 2 48 11 4 Replacing the Lamp 49 119 Arven Tm 50 11 6 Replacing 52 11 7 Replacing the External 52 12 5 53 12 1 _ _ ___
5. Desired Power in Watts X Power Factor X 100 The unit will display power W mW or uW as appropriate See GET Power Factor command description for obtaining LUMEN DYNAMICS Fluorescence Illumination In Control Description Command Response power factor Notes Any decimal places remaining in xxxxx after calculations will be truncated Adding a sixth character to xxxxx will return an error Requiring decimals or extra characters in xxxxx is an indication of going beyond the calibrated range of the System Get Calibration Time ee r xxx r where xxx is the number of calibration hours remaining in ASCII format Clear Calibration r on CLF On Ar Turn Tum CLF Off _____ Off Ar Get Unit Serial Number GSN r where xxxx is the serial number of the unit in ASCII format LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 10 Troubleshooting Service to be completed by qualified repair personnel only 10 1 Error Codes If the X Cite exacte internal monitoring systems identify a problem an error code will be generated Definitions and recommended actions are in the following table Error Description Recommended Action 1 Lamp temperature is too high ER Internal communication failure ES Iris failed to go to home position Internal hardware failure
6. Fluorescence Illumination In Control 12 4 Light Guide Light Delivery Flexible liquid filled light guide 1 5m or 3m in length with a core diameter of 3mm Custom light guides are also available Liquid Light Guide End Fitting Liquid Light Guide Dimensions in mm Core End Fitting Fitting _ Minimum Bend Radius mm dO d1 d2 3 5 9 40 Transport and Storage Conditions Temperature 5 C to 35 C Note Refer to section 5 3 for tips on maintaining liquid light guides in good working condition 12 5 General Height 8 2 20 6cm Depth 13 3 33 8cm Width 7 1 18 0cm Weight 10 8lbs 4 9kg Note X Cite exacte unit dimensions only does not include clearance at front of unit for liquid light guide LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 13 Regulatory 13 1 Product Safety EN IEC 61010 1 2001 oafety Requirements for electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements CAN CSA C22 2 No 61010 1 04 oafety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements UL 61010 1 2004 2 Edition oafety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements IEC Equipment Class Installation Category Pollution Degree 2 Electromagnetic Compatibility EN 61326 1 2006
7. Note If the GUI is in use ensure that the front panel of the unit is unlocked when attempting to use the SHUTTER button 2 LED displays SF1 indicating a shutter failure POWER down the unit wait a few minutes and turn POWER on to the unit If the unit displays a shutter failure again contact your local Lumen Dynamics Service Center to have your unit serviced 10 6 LED Display Failure If the LED display does not light 1 Ifthe fan is functional POWER down the unit wait approximately 20 seconds then POWER it up again 2 If problem persists contact your local Lumen Dynamics Service Center 10 7 Cooling Fan Failure If one or more fans do not work 1 If the LED display is functional POWER down the unit wait approximately 20 seconds then POWER it up again 2 Ifthe problem persists contact your local Lumen Dynamics Service Center LUMEN DYNAMICS Fluorescence Illumination In Control 11 Care and Maintenance Note Unless otherwise specified information in this section applies to all X Cite arc lamp illuminators regardless of which model may be depicted 11 1 General 11 1 1 Er 2 ft X 0 5 11 1 3 11 1 4 11 1 5 A dE gt A F 11 1 7 11 1 8 This section contains installation maintenance and troubleshooting tips to help optimize the lifetime and performance of X Cite exacte units and components This information is intended
8. Failure to communicate with Intelli Lamp Lamp self extinguished etait Shutter Failure Check ventilation filters and outlets to ensure that no blockage exists Restart unit and if problem persists replace lamp If problem continues contact Tech Support See also section 10 7 Restart unit if problem persists contact Tech Support Restart unit if problem persists contact Tech Support Restart unit if problem persists contact Tech Support Check Intelli Lamp connection and restart unit If problem persists try another lamp if problem still persist contact Tech Support Let system cool and restart lamp If it goes out again replace lamp If new lamp continues to self extinguish contact Tech Support Restart unit if problem persists contact Tech Support Lamp installed incorrectly Lamp did not strike The lamp will automatically strike when it has cooled to the optimum striking temperature The lamp has accumulated over 2500 hours Lamp may be near end of life The lamp has reached end of life The lamp will not strike All front panel buttons have been locked to orevent settings from being changed The shutter has failed to return to home position Unit should be restarted If the error repeats contact T ech Support LUMEN DYNAMICS Fluorescence Illumination In Control 10 2 Power Up Failure If the unit fails to POWER up or function properly use the following checklist to e
9. 2600 or TOLL FREE 1 800 668 8752 Any additional optional items purchased to customize the unit will also be present LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 4 2 Front Panel LAMP ON pu LED DISPLAY STATUS LED X Cite E STATUS LED QU e E e INDICATOR 1 LEDS LIGHT GUIDE SEPP Sr UN LIGHT GUIDE HRS b STATUS LED 2 CAL e EN CALIBRATION LIGHT GUIDE STATUS LED PORT xad eee MODE UP DOWN ADJUST BUTTON POWERMETER A i POWER METER CALIBRATION PORT POWER ON OFF SWITCH ACTIVATION CLOSED LOOP je FEEDBACK LED BUTTON Figure 1 Front Panel LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 4 3 Rear Panel Jl TTL INPUT FOR SHUTTER umen Dynamics Group Inc LU M E N Tel 905 ye LE DYNAMICS con Lo ne C e No XCT10 vo e ln s PM Serial No XCT10 0029 CEE CONNECTION FOR SHUTTER nput A 50 60Hz 3 5 2 0A SYNC Fuse lt F5 0A 250V Lamp 200 Watt Mercury Arc ipta Patent Pending Publ No US 2006 0139722 A1 MADE IN CANADA 911 00090 Rev O E Ge FOOT PEDAL AC RECEPTACLE f gt INPUT alice F5 0A 250V DRAWER 1 1
10. IEC 61326 2002 Immunity Testing Electrical Equipment for Measurement Control and Laboratory Use EMC Requirements Part 1 CISPR 11 2004 EN 55011 1998 1 amp A2 Emissions Testing Class A Group 1 Industrial Scientific amp Medical Equipment FCC Part 15 Subpart B Class A Unintentional Radiators CE Marking Council Directive 73 23 EEC Low Voltage Directive C Council Directive 89 336 EEC EMC Directive Council Directive 2002 96 EC WEEE Directive LUMEN DYNAMICS Fluorescence Illumination In Control FCC Class A Didital Device or Peripheral Information to User NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense WARNING Changes or modifications not expressly approved by Lumen Dynamics could void the user s authority to operate the equipment WEEE Directive 2002 96 EC The symbol
11. Plug the foot pedal into the 3 5mm audio style port labelled FP on the back on the X Cite exacte Place the foot pedal on the floor or another conveniently located flat surface Power up the X Cite exacte as normal and wait for the lamp to warm up Press down on the foot pedal until it clicks to open or close the shutter 9 10 Shutter control via TTL 9 10 1 For high speed shutter activation TTL control can also be used The plot below shows typical timing values for the X Cite exacte internal shutter when controlled by TTL Note that these values should be used as a guide only and that actual values will vary slightly from unit to unit TRIGGER SHUTTER RESPONSE SYNC OUTPUT Interval Description Time milliseconds Delay time trigger to start of opening Start of opening to 10096 open 3 Minimum recommended exposure time trigger open to trigger closed Delay time trigger to start of close Start of close to 096 open Note Using values of t8 lt 40ms may result in shutter bounce back and a brief unintentional exposure period LUMEN DYNAMICS Fluorescence Illumination In Control TTL Signal and Shutter Status TL Shutter ow TTL INPUT BNC SYNC OUT BNC Maximum low level 0 8VDC Maximum low level 0 8VDC Minimum high level 2 0VDC Minimum high level 4 5VDC Maximum high level 5 5VDC Maximum output current 500uA Typical input current 800UA
12. basic instructions for using the Objective Plane Power Sensor with the X Cite exacte are provided here For full instructions and safety information refer to the complete Optical Power Measurement System User s Guide 035 003908 7 4 2 Leave the X Cite exacte light guide connected to your microscope as you would during normal operation Keep the X Cite exacte positioned as you would during normal operation 7 4 3 Place the XP750 onto your microscope as described in user guide 035 00390 and connect it to an XR2100 7 44 Attach the Stereo Connection Cable to the port on the right side of the XR2100 under flap in rubber boot and the port on the right side of the X Cite exacte 7 4 5 IMPORTANT The XP750 will calibrate X Cite exacte using data from only one wavelength setting It is important to select the wavelength most applicable to your application prior to calibrating the X Cite exacte using the system The ideal wavelength setting is dependant on your application and typically corresponds to the excitation maxima peak of the fluorophore Please refer to user guide 035 00390 for setting the wavelength being measured by the system 7 4 6 Press the X Cite amp CAL button on the XR2100 to perform an XP750 calibration 7 4 7 The X Cite exacte will display CAL and the XR2100 will display a moving dash while the calibration sequence is running 7 4 8 When the calibration is complete the CAL LED on the X Cite exacte will light u
13. defects in material and workmanship In the event of a claim under this guarantee the equipment is to be sent postage and carriage paid including a description of the fault to the Lumen Dynamics Service Center Returned equipment will not be received without a Return Authorization RA Number issued by the appropriate Service Center In the case of damage caused by wear and tear careless handling neglect by the use of force or in the case of interventions and repairs not carried out by an Lumen Dynamics Service Center the guarantee ceases to be valid This guarantee may not form the basis for any claims for damages in particular not for compensation of consequential damages The warranty is not transferable No warranty is extended to perishable items such as fuses air filters and light guides Any claims for units received with defects in material or workmanship must be reported to an authorized Lumen Dynamics service Center within 30 days from the original date of receipt Replacement Bulb Warranty If the X Cite exacte bulb fails to strike during the warranty period of 2000 hours the bulb will be replaced under warranty or a credit will be applied to the purchaser s account In the event of a claim under this guarantee the lamp is to be sent postage and carriage paid including a description of the fault to the Lumen Dynamics Service Center Returned equipment will not be received without a Return Authorization RA Number
14. for a light guide to degrade prematurely and result a sudden reduction in illumination intensity Bubbles form without warning usually due to overheating and or mechanical stress to the light guide Below are some simple tips to avoid overheating and stressing the light guide The light guide has a minimum bend radius of 1 6 inches 40 0mm Bending coiling the light guide tighter than this radius will result in permanent damage to the light guide Make sure the light guide is cooled properly during use and prevent overheating a Always fully insert the light guide in to the X Cite unit this ensures contact with a heat sink to conduct heat away from the light guide b Never obstruct the air vents on the X Cite unit Vents are located at the rear and underside of the unit Do not remove the rubber feet on the X Cite unit or otherwise reduce block the space between the bottom of the unit and bench top This may compromise airflow through the unit d Ensure that the air being used to ventilate the X Cite unit is approximately room temperature e g do not place the X Cite unit on top of another heat producing instrument e heated environmental chamber is being used for live cell imaging make sure that the X Cite unit and the light guide are located outside of the chamber Do not expose the light guide to extreme temperatures above 35 C below 5 C for extended periods of time during use transport or s
15. of light forcing the light guide to absorb the energy tis 2 3 years old OR has been in use for 4000 6000 hours 2 3 lamp changes LUMEN DYNAMICS PUTTING YOU IN CONTROL X Cite Fluorescence Illumination In Control 11 3Lamps Performance and lifetime 11 3 1 The lamp output spectra and typical output levels over lifetime are shown below Spectral Comparison Power mW 300 350 400 45D 500 550 600 650 700 750 Wavelength nm X Cite exacte HBO 11 3 2 It is normal for the output to decline over the first several hundred hours of use and then stabilize at a level 70 of the initial output for the remainder of the lamp life If the lower power regions of the output spectra are being used it may be desirable to replace lamps more frequently to maximize signal levels exacte Lamp Typical Degradation Relative Intensity Time hours LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control General tips to help maximize lamp lifetime 1 Each time the lamp is ignited the stress on the lamp effectively decreases lamp life by 5 hours therefore it is recommended to avoid shutting down the unit during lunch or short breaks or between users if being used in a multi user lab After lamp ignition avoid turning the lamp off until it has run for a minimum of 20 minutes Avoid attempting to strike a hot lamp note the X Cite s Intelli L
16. system is getting ready e Red icons mean there is an alarm condition LUMEN DYNAMICS Fluorescence Illumination In Control 2 Shutter control with GUI vs front panel buttons The internal shutter can be operated using either method however for the unit to accurately monitor shutter status only one method should be used at a time If the front panel is unlocked the unit assumes the front panel shutter button will be used and the shutter icon on the GUI is disabled If the front panel is locked the system assumes that the GUI control will be used and the front panel shutter button is disabled 3 When icons are ALL grey the X Cite exacte is either disconnected or not communicating properly with the computer 4 Side Panel Tabs shown greyed out in Figure 8 GUI Control Panel will not be operational unless the X Cite Optical Power Measurement System is connected Refer to X Cite Optical Power Measurement System User Guide 035 00390R for instructions 5 GUI Control Panel is collapsed in view below COLLAPSE AND ICONS EXPAND WINDOWS X Cite Control Panel File Data Lag Power SnapShot Power Meter Help LUMEN DYNAMICS Fluorescence Illumination In Control 9 6 Logging Stability Data 9 6 1 When in CLF mode the signal from the feedback loop can be recorded as verification that the set point is being maintained Simply open the Stability Data tab and click on the Record icon to st
17. the ON button 7 Open X Cite exacte shutter the power reading will show on the display e W D p LUMEN DYNAMICS Fluorescence Illumination In Control 7 3 X Cite exacte Calibration using the XR2100 or XR2000 amp Light Guide 7 3 1 7 3 2 7 3 3 7 3 4 7 3 9 7 3 6 oet up the XR2100 to obtain power reading through the light guide input port Refer to section 7 2 or user guide 035 00390 Attach the Stereo Connection Cable to the port on the right side of the XR2100 under flap in rubber boot and the port on the right side of the X Cite exacte Press and release the X Cite exacte CAL button on the XR2100 The X Cite exacte will display CAL and the XR2100 will display a moving dash while the calibration sequence is running When the calibration is complete the CAL LED on the X Cite exacte will light up and the X Cite exacte will display intensity settings in the format XX XX in watts For versions 2 0 and up X XXX or XXX X for milliwatts and uXXX for microwatts WARNING Do not remove the light guide from the Power Meter until calibration sequence is complete While calibrating the shutter will automatically open and close under the control of the power meter LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 7 4 Calibrating X Cite exacte with the Objective Plane Power Sensor XP750 7 4 1 convenience the
18. 12 Technical Specifications 12 1 Electrical Power Supply Input Voltage Current Input Surge Protection Fuse Rating 12 2 Environmental Operating Conditions Ambient Temperature Altitude Atmospheric Pressure Relative Humidity Installation Category Pollution Degree Enclosure Rating Power Factor Corrected Universal Input 100 240VAC 50 60Hz 3 5A max at 120VAC 2 0A max at 240VAC 50A max cold start Power supply Short circuit protection Overvoltage protection 30 5096 above nominal output Overload protection 110 160 of normal rating Dual fuse system each fuse rated at F5 0A 250V 5x20mm type located in the AC receptacle 15 C to 40 C 2000m max 700 to 1060 hPa 1596 to 95 non condensing 2 IPXO Transport and Storage Conditions Temperature Relative Humidity Atmospheric Pressure LUMEN DYNAMICS 40 to 70 C 10 to 100 500 to 1060 hPa Fluorescence Illumination In Control 12 3Lamp A Rim of Lamp Reflector B Reflector C Back Ceramic Mount D Intelli Lamp Connector E Power Connector Lamp Module Lumen Dynamics 200W Arc Lamp Lamp Module Life 2000 hours Warm Up 20 minutes min reference sections 3 1 Glossary of Symbols and 6 1 Powering Up Powering Down CAUTION Use only Lumen Dynamics lamp part number 012 66000R in the X Cite exacte unit LUMEN DYNAMICS PUTTING YOU IN CONTROL
19. 9 11 RS 232 Commands via USB port 9 11 1 This command list is divided into two sections The first section contains the X Cite 120PC command structure The X Cite exacte is compatible with most of the X Cite 120PC commands enabling use of the X Cite exacte with existing X Cite 120PC drivers The second section is the expanded command set with the unique X Cite exacte commands 9 11 2 Many of the commands discussed in this section result in an acknowledgement being sent from the X Cite exacte when the command has been received successfully by the X Cite exacte Otherwise an error message 15 sent by the X Cite exacte 9 11 3 An acknowledgement has a packet structure as defined in the following table Byte Alohanumeric Value J m Nv ________ 9 11 4 error message has a packet structure as defined in the following table Byte OO 9 11 5 The X Cite exacte PC driver includes a virtual COM Port Use this port with the following settings e Baud Rate 9600 Parity No parity Data bits 8 Stop bits 1 Flow Control None LUMEN DYNAMICS Fluorescence Illumination In Control 9 12 X Cite 120PC Command Set Note Command Sets from the X Cite 120PC user guide that are not listed below have been discontinued for X Cite exacte compatibility Command Connect enables PC tt r r control of the unit Get Intensity Level iN Where if x is 0 intensity level of 096 1 intensity level
20. G YOU IN CONTROL Fluorescence Illumination In Control 6 Operation 6 1 Powering Up Powering Down 6 1 1 6 1 2 Lamp Warm Up r The ARC lamp has 3 distinct phases of operation 1 Ignition 2 Warm up Lumen Dynamics recommends 20 minutes of proper warm up and to ensure a stable optical output 3 Stable Operation It is recommended that phase 1 and 2 are not interrupted This can result in shortened lamp life The lamp must be allowed to warm up uninterrupted Ensure that the lamp and light guide have been properly installed and that the lamp housing panel is securely fastened Plug the X Cite exacte unit into a properly grounded AC outlet Warning Turning power on to the X Cite exacte unit without the light guide properly installed in the unit and in the microscope can increase your risk of exposure to UV light Turn on the main POWER switch I located on the front panel and check the fan for airflow As soon as the LED display turns on it will display the version of software currently programmed in the unit X Once the software version level has been indicated the display will reset after several seconds to the next display mode The lamp will automatically turn on within 45 seconds and the LAMP indicator will illuminate The display will flash during the warm up period for approximately 4 minutes The display will stop flashing when the
21. If it still does not strike replace the lamp The LED display indicates the cool message This indicates the lamp is too hot to strike The lamp will automatically strike when it has cooled LUMEN DYNAMICS Fluorescence Illumination In Control 10 4 Low Light Intensity If the light intensity is too low check that 1 The percent iris opening is set high enough Put the unit into COARSE Mode and press the UP button to increase the iris opening See Section 6 7 Adjusting the Light Output 2 here are no foreign substances on the emitting end of the light guide 3 There are no bends kinks or other physical damage to the guide Replace the light guide if there is any physical damage 4 The lamp has been installed correctly See section 5 1 Installing the Lamp Module 5 Check the microscope settings filter cubes ND filters manual stops polarisers and position of camera port beam splitters for anything that may be blocking the light path between the X Cite and eyepieces camera It may be necessary to replace the lamp or to replace the light guide Contact your Lumen Dynamics sales representative for information on purchasing a new lamp or light guide 10 5 Shutter Failure If the shutter does not open check that 1 The light guide is fully inserted the LED above the light guide port will be illuminated green The lamp is warmed up the display is not flashing The iris setting is not at 0
22. RED LIGHT GUIDE 5 STATUS LED LIGHT GUIDE INSERTION LINE Ert s GREEN LIGHT GUIDE STATUS LED LIGHT GUIDE INSERTION LINE Figure 6 Light Guide Position Tip When the light guide is fully inserted the line on the light guide insertion label will be right up against the light guide port Also the LED above the light guide port will illuminate green when POWER 15 on to the unit If the light guide is not fully inserted the LED will illuminate red Note The shutter will not open if the light guide is not fully inserted 5 2 5 To remove the light guide firmly grip the strain relief near the light guide retainer and pull out firmly Note The X Cite exacte is designed for use with a 3mm liquid light guide Lumen Dynamics can not guarantee the performance of the X Cite exacte if using light guides other than those supplied by Lumen Dynamics Note The light guide has a minimum bend radius of 1 6 inches 40 0mm Bending or coiling the light guide tighter than this radius will result in permanent damage to the light guide LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 5 3 Tips to Prevent Premature Degradation of Light Guides 9 3 1 9 3 2 9 3 3 9 3 4 9 3 9 Liquid light guides have a typical useful life of 2 3 years in the X Cite exacte when installed and handled properly The formation of bubbles is one of the most common reasons
23. X Cite Fluorescence Illumination In Control X Cite exacte USER S GUIDE 2260 Argentia Road Mississauga ON L5N 6H7 Canada 1 905 821 2600 www ldgi xcite com Printed in Canada 035 00283 Rev 1 LUMEN DYNAMICS GROUP INC 2260 ARGENTIA ROAD MISSISSAUGA ONTARIO L5N 6H7 CANADA X Cite Fluorescence Illumination In Control X Cite exacte User s Guide Lumen Dynamics Group Inc 2007 2011 All rights reserved No part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language in any form by any means without the prior written consent of Lumen Dynamics Group Inc Every effort has been made to ensure information in this manual is accurate however information in this manual is subject to change without notice and does not represent a commitment on the part of the authors X Cite exacte Control Panel Software Minimum Computer Specifications 300 MHz processor Pentium or equivalent Windows 2000 SP4 or Windows XP SP2 128 MB of system memory RAM 5 MB available storage for software installation 20 MB minimum for data storage SVGA 1024 x 768 resolution 16 bit colour One available USB 1 1 port Trademarks X Cite exacte Intelli Lamp and Closed Loop Feedback are registered trademarks of Lumen Dynamics Group Inc All rights reserved All other product names are trademarks of their respective owners LUMEN DYNAMICS X C
24. __ __ ___ 53 ee EE DI EUNDI UCM 53 ____ ___ ____ 6 6 _ _6_ 6_ _ _ 6_ __ _ 54 m 55 025 ACTA 55 T3 1 cnn 56 13 1 Produet MENOR eee 56 pO MEE gt eee 58 1A WE EE EEEE 59 15 60 15 1 Replacement Parts and 60 LUMEN DYNAMICS Fluorescence Illumination In Control 1 X Cite exacte Message Reference Displays the iris setting which gives relative intensity as Display Message XXX Intensity Setting 96 oercentage of the lamp s maximum output Displays the power setting which gives absolute intensity in terms of watts Displays the power setting which gives absolute intensity in terms of microwatts XX XX Power Setting watts uXXX Power Setting microwatts X XXX e Displays the power setting which gives absolute or Power Setting milliwatts a e XXX X intensity in terms of milliwatts Lamp Hours Displays the hours of use accumulated by the lamp Flashing The lamp is warming up Warm up time is XXX approximately 4 minutes ref s
25. above indicates that this product should not be disposed of along with municipal waste that the product should be collected separately and that a separate collection system exists for all products that contain this symbol within member states of the European Union he equipment that you bought has required the extraction and use of natural resources for its production It may contain hazardous substances that could impact health and the environment In order to avoid the dissemination of those substances in our environment and to diminish the pressure on the natural resources we encourage you to use the appropriate take back systems Those systems will reuse or recycle most of the materials of your end life equipment in a sound way he crossed out wheeled bin symbol indicated above invites you to use those systems e f you need more information on the collection reuse and recycling systems please contact your local or regional waste administration LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 13 2 China RoHS 13 2 1 The following table contains substance information for the X Cite exacte as required by the China RoHS regulations X Cite exacte XCT10
26. ally valid for 100 hours of use If the lamp 15 fairly new ie it has lt 300 hours logged recalibrating more often is recommended since the lamp goes through the most dramatic changes in output during this period 4 If in doubt a Measure the output power with the Power Meter or Radiometer and compare to the calibrated value on the display b Recalibrate the unit prior to any critical experiment LUMEN DYNAMICS Fluorescence Illumination In Control 8 Warnings and Alarms 8 1 1 The X Cite exacte is designed to detect certain conditions that may affect safe or proper functioning These conditions are categorized as one of three alarm types according to the danger they pose to the operator system or experiment 1 Critical Safety continuing may cause harm to the operator or equipment alarm shall not be clearable until Alarm Condition is corrected 2 Function operation may continue but depending on specific alarm condition functionality of unit may be limited or impossible e g lamp too hot to strike 3 Warning warning only operation may continue as normal i Other Actions Light guide is not inserted LIGHT GUIDE Shutter shall LED turns red 3 Critical automatically close and p beep audible Safety be disabled from opening attempt is made to open the alarm until light guide is inserted shutter CLF is engaged with iris open at 95 or higher i e limited ability t
27. amp will prevent this 4 Avoid unnecessary movement and jarring of the lamp especially when it is in operation or hot When changing a lamp a Handle the lamp only by the ceramic areas b Never touch the glass envelope of the bulb inner stem the inner surface or the outer surface of the reflector If touched carefully clean the envelope with alcohol Skin oils can etch the glass and cause premature bulb failure c Wear cotton gloves or powder free latex nitrile gloves when handling any lamp Ensure that air filters are kept clear of dust and debris even a partially blocked filter can reduce lamp lifetime to approximately 2596 of what is normally expected 11 4 Replacing the Lamp Module 11 4 1 The X Cite exacte is designed to operate only with the X Cite exacte lamp module supplied by an authorized Lumen Dynamics distributor When the lamp requires replacing use only Lumen Dynamics Part Description 012 66000R X Cite exacte replacement lamp module Refer to Section 5 1 for lamp module replacement instructions Refer to Section 10 for messages regarding troubleshooting and lamp module replacement Refer to Section 3 1 Glossary of Symbols for lamp warning information Refer to Section 6 1 Powering Up Powering Down powering up down instructions LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 11 5 Air Vents amp Filters 11 5 1 X Cite units have several air vents that are an integral pa
28. art logging data New data points will be added every 10 seconds or longer if acquisition interval is increased The default 10 seconds is the shortest interval available On the plot area the x axis is in minutes y axis 15 in variance from the set point 9 7 Logging Events 9 7 1 The Control Panel will automatically log events such as alarm conditions calibration expiry and CLF turning off User defined events or information relating to the X Cite set point or experimental conditions can also be logged in the data file To log an event 1 Left click on the plot area 2 Type in the event or information up to 100 characters 3 Click OK The information will be logged in the data file for the time point when the dialogue box first opened even if it takes several time points to type in the information 9 8 Exporting Logged Data 9 8 1 To export logged data 1 Click on the X icon in the title bar 2 From the drop down menu choose Export Data 3 Give the file a name click SAVE data will be saved in Comma Separated Values csv format which can be opened in Excel LUMEN DYNAMICS Fluorescence Illumination In Control 9 9 Shutter Control via Foot pedal 9 9 1 For hands free operation the X Cite exacte is equipped with a foot pedal The foot pedal is an alternative to the SHUTTER button and can only be used to open and close the internal shutter To use the foot pedal 1 2 di 4
29. artz end will appear as a bright solid circle you may also be able to see a thin circular outline at the quartz liquid interface b Bubbles at near the quartz end appear as dark spots as small as 0 5mm in diameter or even as larger more defined spheres c Bubbles in the middle of the light guide may not be well defined spots but will appear as dark shadows d In extreme cases where the bubble is blocking the entire diameter of the light guide no light will come through even when pointing the distal end at a light Source LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 100 11 3 5 2 11 2 11 Appearance and output of light guides with bubbles relative to an LLG without bubbles 100 Can a bubbled light guide recover 11 2 12 Yes light guides with small bubbles can sometimes recover Disconnect the light guide from the X Cite unit and leave the light guide undisturbed on a shelf for 1 2 weeks For this to be effective it is important to catch the bubble when it is small When should light guides be replaced 11 2 13 It is usually time to replace a light guide when llumination is low and replacing the lamp does not improve brightness Dark uneven areas become visible in the field of view a bubble is blocking part of the light A section of the light guide becomes noticeably warmer than the rest of the guide a bubble is blocking transmission
30. bsolute mode calibrated in watts AND a PC is used to read power settings from the unit via X Cite GUI commands in hyperterminal commercial software or custom software the power values being reported to the PC may not be identical to those on the X Cite exacte display screen This would be an indication that an r2 0 or higher X Cite is being used with software drivers designed for an X Cite exacte with an earlier version of embedded code Contact the software driver developer to request an update Identify enables the extended command set of the X Cite exacte The extended command set are all commands listed in this table Enable PC Shutter Control enables PC control of the internal shutter via serial port commands Disable PC Shutter Control disables PC control of the internal shutter via serial port commands Disconnect PC disconnects all control from the PC for the X Cite exacte Get Unit Status Xxx VY where xxx is the status of the unit The number returned is bitwise and is decoded as follows bit 15 Iris Moving 1 Iris Movement complete 0 Iris Moving bit 14 CLF Engaged bit 10 Light guide inserted bit 8 X Cite exacte communication mode bit 7 Power or Intensity Mode 1 LUMEN DYNAMICS Fluorescence Illumination In Control Power Mode 0 Intensity Mode bit 5 Lock Bit 1 front panel locked 0 front panel unlocked bit 4 Lam
31. d Grey not Calibrated Indicates hours logged on lamp T Lamp Hours green lt 2500 hours yellow gt 2500 hours make sure there is a spare lamp on hand red gt 4000 hours lamp is off due to age Indicates light guide position Light Guide Green Light guide present Light guide not present Click to open close shutter Shutter note lock front panel to use see note 2 below Left mouse click 2 increase by 196 increment intensity Right mouse click decrease by 1 increment Left double click mouse dialog box enabling data input of desired intensity or Engage disengage CLF mode Closed Loop Grey OFF Feedback Green ON if clicked ON intensity will freeze click again to turn off Lock unlock the front panel controls on the unit Green front panel of X Cite exacte locked Lock unlock Grey front panel of X Cite exacte unlocked Shutter will not activate from the control panel if the front panel is not locked Click to turn lamp on off color indicates status Green ON click to turn lamp on Lamp Grey OFF click to turn lamp off Yellow Up lamp is and is Record data on graph variance set point Qj Pause Pmsereomindta Stop Stop recording data Plot Event Left click on graph area to log an event Notes 1 Icon background colour code e Green icons mean status is OK and or the function is engaged e Yellow icons mean a warning or that the
32. d by the X Cite exacte is sufficient to ignite flammable substances Therefore when the unit is operated unattended in an automated environment an alarm function must be provided by the user to indicate a malfunction in the associated equipment used Warning LAMP CONTAINS MERCURY Manage in Accord with Disposal Laws see www lamprecycle org or 1 800 668 8752 Danger Exposure to Mercury represents a health hazard to humans When unpacking or installing the lamp always wear protective clothing and a face mask Operate the lamp inside the X Cite exacte housing only This prevents direct viewing of the arc and in the case of lamp bursting contains the lamp particles In the rare instance in which a lamp bursting occurs and the mercury content is released the following safety precautions are recommended all personnel should be immediately evacuated from the area to prevent inhalation of the mercury vapour The area should be well ventilated for a minimum of 30 minutes Prior to clean up ensure an approved for mercury respirator mask and non porous gloves such as latex or rubber are used After the lamp housing elements have cooled the mercury residue should be collected with the use of a special absorbing agent available from laboratory equipment suppliers Listed below are examples of internet web sites for obtaining Mercury Spill Kits htto www rosshealthcare org Mercon spill kits htm htto www coleparmer ca catalog product_inde
33. down completely Caution Any electronic equipment connected to the X Cite exacte must be comply with the requirements of EN IEC 60950 Cleaning Clean the exterior of the unit with a slightly dampened cloth and simple water detergent solution only LUMEN DYNAMICS Fluorescence Illumination In Control 4 Getting Started 4 1 System Components Carefully unpack the unit and accessories Ensure that all components are present otore the packing material for future use Box Contents FP Cei cerunt 2 Lamp Module X Cite exacte 3 Microscope Adapter if me me meme nnns 4 Liquid Light Guide 3mm x 1 5m 3mm x 3 0 5 Power IEC shielded and 6 CD ROM X Cite exacte Control Panel Software and User Guide Translations 7 Quick Start Guide X Cite 8 Foot Pedal Switch ASSEMDIy ccc cece cece eee e cece eee ee eens e tees eee eee enne nnn nnns 9 USB Cable 1 10 Hex Key 3mm Lamp access If your packaged unit is missing any of the above components call Lumen Dynamics at 905 821
34. e for the software Click Next to continue Select Install from a list or specific location Advanced to locate the driver and click Next 7 Check off Include this location in the search and browse to the location on your hard drive where you unzipped the files to in step 1 Click Next 8 The X Cite exacte has a virtual COM port for support of the X Cite 120PC communication protocol Another piece of hardware will be detected follow steps 5 7 again 9 When set up is complete the X Cite exacte icon will automatically appear on computer desktop LUMEN DYNAMICS a p gt Fluorescence Illumination In Control 9 4 SIDE PANEL TABS OPTICAL DATA STATUS DATA CALIBRATION DATA DATA LOG POWER SNAPS SHOT STABILITY DATA Graphical User Interface Panel X ICON X Cite Control Panel File Power SnapShot Power Meter Help 0 00 1 0 2 0 3 0 4 0 5 0 6 D 7 0 8 0910 DATA LOG ICONS STATUS ICONS FUNCTIONS ICONS Figure 8 GUI Control Panel LUMEN DYNAMICS Fluorescence Illumination In Control 9 5 Icons and Functions ICON DESCRIPTION NAME ____ an alarm state Alarm Click to silence audible alarm Red Alarm on Indicates lamp temperature status IN Yellow 2 Lamp running hot Red Lamp off excessive heat Indicates calibration status Calibration Green Calibrate
35. e only when the X Cite exacte has been calibrated with an X Cite Radiometer or X Cite Power Meter see section 7 and the CAL LED on the front panel is on LUMEN DYNAMICS Fluorescence Illumination In Control 6 6 2 To toggle between Relative and Absolute intensity modes in a calibrated system Ensure that the display is showing an intensity value I e not in Lamp Hours Mode 6 6 3 Press MODE and release after 1 second NOTE Some rounding may occur when toggling between relative and absolute display modes unless the iris setting is a multiple of 596 the calibration points 6 7 Adjusting Light Output 6 7 1 The X Cite exacte includes an iris to adjust the level of illumination Adjustments can be made in increments as small as 196 of maximum output Since there are 100 individual intensity steps for convenience there is the option of adjusting in either COARSE or FINE mode 6 7 2 Press the MODE button to select either the COARSE or FINE mode which will be indicated by the LED s to the right of the display 6 7 3 Use the UP and DOWN buttons to step through the intensity settings Size and unit of the step depends on whether the unit is operating in relative or absolute mode Step Size in Step Size in RELATIVE Mode ABSOLUTE Mode COARSE 10 Value in watts corresponding to 10 0 01 W 6 8 Viewing Accumulated Lamp Hours 6 8 1 The X Cite exacte system automatically accumulates the number of h
36. ection 6 1 Lamp installed incorrectly Lamp Error Lamp did not strike See section 10 3 The lamp will automatically strike when it has cooled to the optimum striking temperature See section 10 3 Old Lamp The lamp has accumulated over 2500 hours Lamp may be near end of life New Lamp Required has reached end of life The lamp will not All front panel buttons have been locked to prevent settings from being changed The shutter has failed to return to home position Unit Shutter Failure should be restarted If the error repeats contact Tech Support Check ventilation filters and outlets to ensure that no blockage exists Restart unit and if problem persists replace lamp If problem continues contact Tech Support Internal communication failure Restart unit if problem persists contact Tech Support Iris failed to go to home position Restart unit if problem persists contact Tech Support Internal hardware failure Restart unit if problem persists contact Tech Support Check Intelli Lamp connection and restart unit If problem persists try another lamp if problem still persist contact Tech Support Let system cool and restart lamp If it goes out again Lamp self extinguished replace lamp If new lamp continues to self extinguish contact Tech Support Internal system error Restart unit if problem persists contact Tech Support See Section 10 for troubleshooting Alternating old bulb Alternating e
37. ed on to the unit 5 1 10 Replace the hex tool in the clips underneath the system for future use pes Warning This unit is designed for bench top use only Always ensure that the unit is operated on a hard stable surface This will prevent obstruction of the bottom chassis ventilation openings obstruction of these openings could result in a possible over heating condition Do not attempt to remove tamper with the rubber feet B located on the bottom of the unit s 5 2 Inserting Removing Light Guide 5 2 1 Ensure the power is not connected to the X Cite exacte unit 5 2 2 Ensure that the protective end caps are removed from both the input and output ends of the light guide prior to installation 5 2 3 Remove plastic plug if present from the light guide port on the front panel of the X Cite exacte 5 2 4 Holding the light guide by the grey sleeve and strain relief portion only insert the light guide into the light guide port Push the light guide in until it seats with a second positive click Note Never grip the light guide during installation or removal in a place other than the reinforced strain relief portion of the light guide LUMEN DYNAMICS Fluorescence Illumination In Control x cite x cite exacte exacte
38. es and how to use them Thank you for choosing X Cite LUMEN DYNAMICS Fluorescence Illumination In Control 3 Safety 3 1 Glossary of Symbols A N CAUTION Risk of danger consult accompanying documents DA WARNING Eye damage may result from directly viewing ultraviolet light 7 protective eye shielding and clothing must be used at all times lt p Input Output Signals 6 Input Signal CAUTION Hot Surface LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 3 2 Safety Precautions gt gt gt gt gt gt The X Cite exacte is equipped with two safety sensors to protect the user from accidental UV exposure In addition please observe the following precautions during use This series of cautions warnings and dangers relate to the operation and maintenance of the X Cite exacte They are also presented throughout this User s Guide where necessary Warning Eye damage may result from directly viewing the light produced by the lamp used in this product Always use protective eyewear and turn the lamp off before removing cover Caution Never look into the light emitting end of the light guide The light could severely damage the cornea and retina of the eye if the light is observed directly Eye shielding must be used at all times as well as clothing to protect exposed skin Warning Always make sure the light gu
39. event excessive bending and or crushing of the light guide against walls Minimum bend radius to prevent immediate damage to the LLG is 40mm however a bend radius of at least 75mm is recommended for light guide while in use Sharper bends can cause heat to build up and cause problems longer term LUMEN DYNAMICS PUTTING YO ROI Fluorescence Illumination In Control 11 2 4 Always place the X Cite unit close enough to the microscope so that there 15 some slack in the light guide and no sharp bends 11 2 5 Never leave an endcap on the output end of the light guide when the other end 15 connected to the X Cite unit if the unit is turned on in this condition the cap will overheat melt and or permanently discolour the quartz end of the light guide 11 2 6 Do not expose the light guide to extreme temperatures above 35 C below 5 C for extended periods of time during use transport or storage this may cause degradation of the seals and allow air bubbles to form in the liquid What does a bubble look like 11 2 7 Depending on the size and location in the light guide a bubble may or may not be obvious To check for bubbles 11 2 8 Disconnect the LLG from the X Cite and microscope adapter 11 2 9 Hold one end towards a bright window or overhead room light DO NOT use an X Cite or any other focused light source for this test 11 2 10 Look at the quartz at the other end of the LLG a Bubble free qu
40. h this User Guide This interface will 1 Permit computer control of all functions normally available with the front panel buttons 2 Identify any alarm condition by changing the colour of the corresponding icon 3 Display and record real time intensity data from the X Cite in terms of variance from the set point system parameter changes user defined events 9 2 Installation from CD Note This procedure will install both the GUI and the virtual COM port driver 9 2 1 Insert the CD into the CD ROM drive Auto run will automatically start the set up routine If set up is not automatically initiated open the exacte folder on the CD and double click setup exe 9 2 2 Follow set up wizard instructions 1 Do not remove the CD Connect the X Cite exacte to the computer s USB port using the supplied USB cable 2 he X Cite exacte device will be detected select automatic installation When installed successfully message appears click OK 3 Remove the CD the X Cite exacte icon will automatically appear on your computer desktop 9 3 Installation from ZIP file Note This procedure will install both the GUI and the virtual COM port driver Unzip files and save to a folder on your computer Double click the file setup exe within that folder Follow set up wizard instructions Connect the X Cite exacte to the USB port of your computer The new hardware found wizard will appear select No to search windows updat
41. hutoff within 10 15 minutes of the unit being powered on due to lack of adequate cooling 11 5 8 A partially clogged filter may allow enough airflow for continued operation but not for optimal cooling In this case the result is usually dramatically reduced lamp life e g 400 hours instead of 2500 hours LUMEN DYNAMICS Fluorescence Illumination In Control 11 6 Replacing the Air Filter 11 6 1 The external air filter is located under the front face plate of the X Cite exacte 11 6 2 Turn off the main POWER switch and remove the AC POWER cord from the unit 11 6 3 Gently slide and pull out the filter 11 6 4 Push in the replacement filter so that it sits flat in place 11 7 Replacing the External Fuses 11 7 1 The external main fuses are located in the fuse drawer which is located in the AC inlet module on the rear panel 11 7 2 Turn off the main POWER switch and remove the AC POWER cord from the unit 11 7 3 Gently pull out the drawer with the aid of a flat head screwdriver 11 7 4 Carefully lever one end of each fuse up from its retaining clip with a small flat head screwdriver and lift it out 11 7 5 Replace the blown fuse s only with the same type and rating F5A 250V The rear compartment must contain two active fuses 11 7 6 Close the fuse drawer 11 7 7 Reconnect the AC POWER cord LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control
42. ide is properly inserted into the X Cite exacte and the microscope prior to turning on power to the unit This will minimize the risk of exposure to the UV light Caution To prevent damage degradation of the light guide always allow adequate clearance at the front of the X Cite exacte unit to prevent kinking or excessive bending Warning To reduce the risk of fire or shock always replace the fuses with the same type and rating Warning Disconnecting of main supply source is only possible by unplugging the power cord Danger This unit contains HIGH VOLTAGE components It is recommended that ONLY QUALIFIED TECHNICAL PERSONNEL perform any testing or repairs described in this manual Disconnect the AC power cord from the unit before opening the cover of this unit All cover screws must be replaced prior to applying power to the unit or safety of the unit will be impaired LUMEN DYNAMICS Fluorescence Illumination In Control A gt Monitoring the unit during manual operation The level of UV and visible energy supplied by the X Cite exacte is sufficient to ignite flammable substances During manual operation the unit must be attended at all times by a qualified operator The unit must not be left unattended while turned on If an operator leaves the work area of the unit the lamp power switch must be turned off Monitoring the unit during Automated operation The level of UV and visible energy supplie
43. is in position to fit into the spring clamp Slide the lamp until it snaps into the spring clamp The leading edge of the reflector lamp rim should fit snugly into the lamp holder recess SPRING CLAMP LUMEN DYNAMICS Fluorescence Illumination In Control 5 1 6 Locate the 4 pin Intelli Lamp sensor connector at the rear of the lamp module and connect it to its mate located on the top of the lamp housing wall Tip The Intelli Lamp connector will only attach in the correct orientation If you are having difficulty attaching the connector try rotating it by 180 the Intelli Lamp connector is not installed correctly the lamp will not strike and the bulb message will display when POWER is turned on to the unit INTELLI LAMP CONNECTOR POWER CONNECTOR Figure 5 Lamp connections 5 1 7 Locate power connector with two leads and connect it to its mate located on the side of the lamp housing wall CENTER BAR AND LEAD WITHIN CUT OUT LUMEN DYNAMICS Fluorescence Illumination In Control 5 1 8 Ensure the lamp anode cooling fin bar and lamp power lead at the front of the lamp are centered within the lamp holder cut out Rotate the lamp as required 5 1 9 Replace the lamp housing side panel and tighten the fastening screw Note If the lamp housing panel is not secured completely the lamp will not strike and the bulb message will display when power is turn
44. issued by the appropriate Service Center Lamps must be purchased from an authorized Lumen Dynamics Representative or Distributor to be eligible for the warranty replacement This warranty is non transferable In the case of damage caused by careless handling neglect by the use of force or in the case of interventions and repairs not carried out by an Lumen Dynamics oervice Center to the X Cite exacte system the guarantee ceases to be valid Returning equipment to Lumen Dynamics 1 Please make a note of the problem encountered the steps followed to isolate the problem and the result of any trouble shooting steps taken 2 Contact the nearest Lumen Dynamics Service Center to obtain a Return Authorization Number For your convenience RA numbers can also be requested on line at http www ldgi xcite com support need serviced ph LUMEN DYNAMICS Fluorescence Illumination In Control 3 Follow shipping instructions provided by the service technician The unit should be returned in its original packaging if possible Please do not ship the unit with the lamp installed 15 Contact Information www ldgi xcitestore com Lumen Dynamics Tel 905 821 2600 Fax 905 821 2055 1 800 668 8752 USA and Canada x cite Idgi com www ldgi xcite com 15 1 Replacement Parts and Accessories Replacement parts and accessories can be purchased directly from Lumen Dynamics For ordering and pricing information con
45. ite Fluorescence Illumination In Control Table of Contents 1 X Cite exacte Message 1 MEIER OCUG tO 2 EEUU 3 SM EEMECIo 5 14182 EU m 3 32 dece ac m TTE 4 A 7 41 System 5 7 LT FON gt 8 Ao 9 De Installation Set up MEER s 10 5 1 Installing Lamp 10 5 2 Inserting Removing Light Guide 13 5 3 Tips to Prevent Premature Degradation of Light Guides 15 5 4 Installing the Collimating Adapter 16 EE 18 6 1 Powering Up Powering Down seeeeseeseeeeeeennnnn ener 18 6 2 Quick Start Illuminating a 19 6 3 Front Panel Button Functions 20 MAGICA ONS 20 6 5 Display Format Reference eR E Ine s da E 21 6 6 Output Intensity nnne nnns 21 6 7 Adjusting Light
46. liminate the most common causes of problems Check that 1 The AC POWER cord is securely plugged into a functional AC wall plug 2 AC POWER cord is securely plugged into the AC inlet on the rear of the unit 3 The main POWER switch is in the ON position 4 Check that the ventilation openings on both the bottom and rear of the unit are not blocked If the unit still does not power up N 5 Check both main power fuses by first disconnecting the power cord Then carefully remove the fuse drawer assembly below the AC inlet on the rear of the unit If the fuse s is are open replace with the same type 4A 250 V Fast acting 10 3 Lamp Strike Failure If the LED display lights and the fan starts but the lamp will not turn on check for the following 1 The LED display indicates the bulb message and the system begins to beep This indicates that no lamp is detected Check if the lamp has been installed correctly Refer to Section 5 1 Installing the Lamp Module 2 he LED display indicates the bulb message after approximately 45 seconds and the system begins to beep This indicates that the lamp has failed to strike It may be a result of the lamp reaching end of life or that the lamp housing panel is not secured properly in place Press the MODE button to clear the audible alarm Turn power off to the unit Check that the lamp housing panel is secured properly Wait a few minutes and turn the power on to the unit
47. nd bulb Front Panel Locked Lamp temperature is too high Failure to communicate with Intelli Lamp m LUMEN DYNAMICS Fluorescence Illumination In Control 2 Introduction The X Cite exacte is a revolution in fluorescence microscopy illumination Like the previous X Cite illuminators the X Cite exacte has the convenience of pre aligned long life Intelli Lamps easy installation and compatibility will all major microscope brands With additional features such as Closed Loop intensity adjustment in 1 increments a high speed internal shutter and a calibrated output option which is compatible with the X Cite Optical Power Measurement System the X Cite exacte reaches a new level of performance that makes it the ideal choice for quantitative comparative and live cell imaging 1982 Lumen Dynamics formerly EXFO has combined next generation optical engineering state of the art electronics and fibre optics to produce sophisticated technologies that employ light Today Lumen Dynamics is a leading developer of light based systems for sectors ranging from manufacturing to bio medicine and we are unmatched in our commitment to quality and service The X Cite exacte is a high quality product manufactured in accordance with ISO 9001 CE marked and certified to UL China RoHS and CSA standards We suggest that you read this manual to discover all its featur
48. o compensate Mul for degradation CLF is engaged and iris has Audible beeping Operation in non CLF reached 10096 open blinking CLF LED mode will continue End of calibration period approaching EE Warning e g 12 hours left Galbialionhasexcied CAL LED turned Continue operating in off relative Lamp failure to strike Display BULB Function Lamp too hot to strike Display COOL Function re strike lamp when cool Audible beeping Critical If failure to close turn off Shutter failure Heed display SF1 Safety lamp Lamp overheated Display E1 Turn off lamp Safety Lamp seli extinguish idis Display E6 Fan blower shall shut off passive failure Safety LUMEN DYNAMICS Fluorescence Illumination In Control 8 2 Clearing Alarms 8 2 1 8 2 2 The X Cite exacte provides an audible alarm to alert the operator of various error conditions To silence the audible alarm 1 Manually Press the front panel MODE button 2 Remotely a From the Graphical User Interface GUI by clicking the Alarm icon b From a PC through I O port when the aav command is sent to the X Cite exacte LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 9 External Control 9 1 Graphical User Interface GUI 911 A Graphical User Interface GUI for use with the X Cite exacte is supplied on the CD included wit
49. of 1296 2 intensity level of 25 3 intensity level of 5096 4 intensity level of 10096 Get Lamp Hours hh oor where is the number of lamp hours Get Software Version vv r xx r where xx is the version number of the software multiplied by 10 Get Unit Status where xxx is the status of the unit The number returned is bitwise and is decoded as follows bit 5 Lock Bit 1 front panel locked 0 front panel unlocked bit 4 Lamp Ready Bit 1 lamp is ready 0 amp is not ready bit 3 Home Bit 1 fault 0 pass bit 2 Shutter Bit 1 shutter is opened 0 shutter is closed bit 1 Lamp Bit 1 lamp is ON 0 lamp is OFF bit 0 Alarm Bit 1 alarm is ON 0 alarm is OFF Set Intensity Level where x is the desired intensity level If x is 0 Intensity is 0 1 Intensity is 12 2 Intensity is 25 3 Intensity is 5096 4 Intensity is 10096 zz Ar ssv Ar bb r Ar aar INe Ar nnr Ar LUMEN DYNAMICS Fluorescence Illumination In Control 9 13 X Cite exacte Command Set Compatibility Note 1 Commands marked with are not compatible with X Cite exacte units with embedded code versions lower than r2 0 Version number is indicated on the display screen during start up and can also be obtaining using the Get Software Version command 2 If X Cite exacte is used in a
50. ours for which the lamp has been on and shows this information on the LED display The lamp hours are shown when the unit is in Lamp Hour mode 6 8 2 To put the X Cite exacte in Lamp Hour Mode press the MODE button until the HRS LED on the front panel is illuminated The LED display will show four digits with a flashing decimal point at the end XXXX The number shown here will be the total number of hours the lamp has been in operation LUMEN DYNAMICS Fluorescence Illumination In Control 6 9 Closed Loop Feedback CLF 6 9 1 6 9 2 6 9 3 6 9 4 The X Cite exacte is equipped with Closed Loop Feedback CLF control to ensure that from the beginning to the end of an experiment the illumination level remains constant When CLF is enabled the system will automatically compensate for any fluctuation or degradation in light intensity by adjusting the iris position For best results allow the lamp to warm up and stabilize to its normal operating temperature about 30 minutes To enable CLF Set the intensity to the desired level Press and hold the MODE button then press and release the UP button The CLF light will turn on Note UP must be released before MODE treat MODE like a shift key To disable CLF Press and hold the MODE button then press and release the UP button The CLF light will turn off Even over several days the output power will be maintained within 2 of the set
51. p and the X Cite exacte will display intensity settings in the format XX XX in watts X XXX OR XXX X for milliwatts and uXXX for microwatts 7 4 9 X Cite exacte internal software version 2 0 or higher is required to display the lower power measurements from XP750 calibration Software version is displayed during X Cite exacte start up sequence Contact Lumen Dynamics if a software upgrade is required Note the shutter will be in the closed position when the calibration routine is complete LUMEN DYNAMICS Fluorescence Illumination In Control 7 5 To Clear a Calibration The X Cite exacte calibration will automatically be cleared under any of the following circumstances 1 The light guide is pulled out 2 The lamp module is replaced The calibration has been in use for more than 100 hours NOTES 1 For best results allow the lamp to run at least 30 minutes before running a new calibration As the lamp heats up and the internal burner pressure changes lamp output will also change For a calibration to be accurate the lamp should be at its normal operating temperature 2 The number of bends in the light guide as well as their bend radii can have an effect on light transmission For best results maintain the same light guide configuration shape that is present during normal use should be used when calibrating If equipment is rearranged verify or redo the calibration 3 Calibrations are typic
52. p Ready Bit 1 2 lamp is ready 0 lamp is not ready bit 3 Home Bit 1 fault 0 pass bit 2 Shutter Bit 1 shutter is opened 0 shutter is closed bit 1 Lamp Bit 1 lamp is ON 0 lamp is OFF bit 0 Alarm Bit 1 alarm is ON 0 alarm is OFF Ar Ar Ar Set Intensity Level dxxx r where 15 Ar the desired intensity percentage from O to 100 Get Intensity Level da Xxx WV where xxx is the intensity percentage in ASCII format Change Power Mode qq r Ar changes between intensity and power display if calibrated x Get Power Factor 27 where xxxxx is the power factor to be applied to Output Power values to convert the into watts or to convert a desired setting into an integer that can be interpreted by the unit The power Factor will be returned as either 100 1000 10000 See Get Output Power commands for proper use of this number Power factor is redefined during each calibration process and should never be assumed to be a constant Dp XXXxx V where xxxxx is a number in ASCII format that can be used to calculate power output in watts Power in watts 2 xxxxx Power Factor X 100 See Get Power Factor command description for obtaining power factor Get Output Power x Change Output Power where xxxx is the desired output power setting converted to an integer that can be interpreted by the unit
53. point Set point the output level at which CLF is enabled For verification of the actual output power the variance from setpoint can be logged over time and exported as a data file see section 9 for additional information Important Notes about CLF Since CLF uses the iris to make adjustments to output power CLF can not be used if iris position is 10096 open Similarly if the initial set point is 9596 and over a continuous multi day experiment the iris needs to be opened to 100 to maintain constant intensity CLF will not be able to compensate for further drops in power Warning signals while CLF is engaged 1 If the iris reaches 95 open or more the CLF LED will start to blink 2 If the iris reaches 10096 open the CLF LED will blink and alarm will beep until CLF is disengaged NOTE If setting CLF while in absolute power mode display in watts check the actual iris setting first to be sure that there is room for output adjustments to be made What will the display show when CLF is engaged In Relative Mode Actual iris position i e value will update iris is adjusted in CLF In Absolute Mode Output power i e value will not change since this is the set point NOTE The intensity setting can not be changed while CLF is engaged however the MODE button can be used as usual to toggle between display modes to view hours iris position and power set point LUMEN DYNAMICS Fluorescence Ill
54. rt of the cooling system and overall lamp performance Proper cooling ensures that lamps operate at optimal temperature and pressure for output power spectrum lamp life light guide life and safety Ensuring adequate cooling 11 5 2 Never obstruct the air vents on the X Cite unit Vents are located at the rear and underside of the unit Note Arrows indicate direction location of air flow In earlier X Cite models vents were located on the side underneath the lamp access panel 11 5 3 Always leave clearance for air flow between the X Cite and walls or other equipment Do not remove the rubber feet on the X Cite unit or otherwise reduce block the space between the bottom of the unit and bench top This may compromise airflow through the unit LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 11 5 4 Ensure that the air being used to ventilate the X Cite unit is approximately room temperature e g do not place the X Cite unit on top of another heat producing instrument 11 5 5 If a heated environmental chamber is being used for live cell imaging make sure that the X Cite unit and the light guide are located outside of the chamber 11 5 6 Periodically check the air filter at the air intake vent for debris Replace or clean as necessary What happens if filters are not clean 11 5 7 A complete filter blockage or airflow obstruction generally results in an automatic lamp s
55. scard the existing mounting hardware since it will be used to secure the collimating adapter to the microscope Remove the collimating adapter from its packaging Note The collimating adapter has been set at the factory No adjustments by the customer are required however some adapter models are outfitted with centering set screws for longer light path applications Separate instructions are included with these models Insert the flange portion of the collimating adapter into the lamp port of the microscope Using the existing hardware tighten the screw s until the flange is fully secured Note The installation of the collimating adapter will vary with microscope models Remove the protective cap from the output end of the light guide Insert the light guide into the input portion of the collimating adapter until it is flush with the adjustable insert Secure the light guide to the collimating adapter by tightening the thumbscrew Do not over tighten Note The light guide has a minimum bend radius of 1 6 inches 40 0mm Bending or coiling the light guide tighter than this radius will result in permanent damage to the light guide LUMEN DYNAMICS Fluorescence Illumination In Control Warning Turning power on to the X Cite exacte unit without the light guide properly installed in the unit and in the microscope can increase your risk of exposure to UV light LUMEN DYNAMICS PUTTIN
56. tact the inside sales department at x cite 2ldgi com www ldgi xcite com 1 800 668 8752 850 00023R Hex Key 3mm lamp access cover 010 00245R Optical X Cite XR2100 Optical Power Meter 010 00242R Power Measurement uu System X Cite XP750 Objective Plane Power Sensor 010 00241R 031 00022 X Cite exacte CD Users guide 035 00287 X Cite exacte Quick Start English LUMEN DYNAMICS
57. temperature to increase above optimum temperature significantly reducing lamp life When necessary clean the light emitting end of the light guide using an optical cleaning solution Cleaning of unit is not required however if cleaning is desired disconnect the AC power cord from the unit and use only a water and simple detergent solution Ensure that cleaning solution does not come in contact with any optical moving mechanical or electrical parts LUMEN DYNAMICS Fluorescence Illumination In Control 11 2Liquid Light Guides Proper handling and installation of liquid light guides 11 2 1 11 2 2 11 2 3 Liquid light guides have a typical useful life of 4000 hours of operation when handled properly and installed in a well maintained X Cite exacte The formation of bubbles is one of the most common reasons for a light guide to degrade prematurely and result in a sudden reduction in illumination intensity Bubbles can form without warning and if they occur within the first 1500 to 2000 hours of use is it typically due to overheating and or mechanical stress to the light guide Below are some simple tips to avoid overheating and stressing the light guide Always fully insert the light guide in to the X Cite unit i e up to the white line this ensures contact with a heat sink to conduct heat away from the light guide Always allow adequate clearance at the rear of the X Cite unit and microscope to pr
58. ting which gives relative Intensity Setting intensity as percentage of the lamp s maximum output Displ h r n hich gives absolute XX XX Power Setting watts PE A PONE Seid intensity in terms of watts Displ h n hich Power Seting isp ays t e power setti g which gives absolute intensity in terms of microwatts X XXX Displays the power setting which gives absolute Seng intensity in terms of milliwatts M X y XXXX Displays the hours of use accumulated by the lamp Flashing The lamp is warming up Warm up time is XXXX approximately 4 minutes 6 6 Output Intensity Modes 6 6 1 The X Cite exacte can be operated in two different output modes Relative and Absolute Relative Mode Intensity level is adjusted and displayed as a percentage of the lamp s current maximum output When operating in relative mode intensity is displayed using three digits XXX on the display screen Absolute Mode Intensity level is calibrated in watts and can be adjusted and displayed in terms of the actual output power in watts When operated in absolute mode intensity is displayed using four digits with a decimal point uXXX X XXX or on the display screen Formatting of the digits will indicate whether units are watts milliwatts microwatts Scale will auto adjust as appropriate NOTE Absolute Mode is only accessibl
59. to supplement the detailed installation and operating instructions found in the X Cite User Manuals Topics covered include liquid light guides lamps and air vents the components most likely to affect performance over time and require maintenance or replacement Note that the lifetime information on components 15 for typical or average conditions Depending on the imaging application more frequent replacement may be required for example if a fluorophore s signal is low to begin with or is excited by a low power portion of the X Cite lamp s output spectrum Operate the unit in a well ventilated area with at least six inches clearance at the rear of the unit for proper air flow Do not place any objects below the unit between the feet as this will restrict airflow through the bottom of the front face plate For safe operation use only a grounded outlet Avoid physical shocks or jarring to the unit especially while the unit is operating ouch sudden movements reduce the lamp module life The lamp module must be operated for a minimum of 20 minutes each time it is turned on to prevent damaging the lamp Increasing the time between turning the lamp module on and off will maximize lamp life Replace the air filter found under the front face plate frequently to ensure unrestricted air flow It is recommended as a minimum that the air filter be replaced every time the lamp module is replaced Note restricted airflow can cause the lamp
60. torage this may cause degradation of the seals and allow air bubbles to form in the liquid Never kink bend crush or stretch the light guide this type of mechanical stress may cause bubbles to form in the liquid and or damage to the outer sheath a Always allow adequate clearance between the light guide port of the X Cite unit and other objects to prevent excessive bending b Place the X Cite unit close enough to the microscope so that there is some slack in the light guide and no sharp bends LUMEN DYNAMICS Fluorescence Illumination In Control 9 3 6 5 3 7 Never leave an endcap on the output end of the light guide when the other end is connected to the X Cite unit if the unit is turned on in this condition the cap will overheat melt and or permanently discolour the quartz end of the light guide While the X Cite unit is on but not in actual use i e during sample preparation or between time lapse time points close the iris shutter on the X Cite this reduces unnecessary UV photon load on the liquid light guide Using only the shutter stop in the microscope itself protects the specimen but energy is still passing through the light guide 5 4 Installing the Collimating Adapter 9 4 1 9 4 2 5 4 3 5 4 4 5 4 5 Ensure that the power is on the X Cite exacte unit The existing lamp housing must be removed from the microscope before the collimating adapter can be mounted Do not di
61. tween relative intensity and MODE absolute power display modes if unit is 1 second calibrated UP Press and release Silences audible alarm during audible alarm Increases intensity by 1 step Press and hold Increases intensity continuously DOWN Decreases intensity by 1 step Press and hold Decreases intensity continuously ________ Engage disengage Closed Loop Feedback MODE UP i teat MODE like Mode disables UP DOWN buttons from shift key being used to adjust intensity 6 4 LED Indicators LED Indicator _ Status Description On Green The light guide is fully i LIGHT GUIDE On Green e 9 U The light guide is not fully inserted LAMP e lamp The lamp is off OPEN COARSE The shutter is open The shutter is closed The unit is in coarse adjustment mode LED display indicates current intensity setting The unit is in lamp hours mode LED display indicates accumulated lamp hours calibrated with Radiometer or Power Meter EMEN TE _______ Feedback Of j ClosedloopFeedbackisnotengaged The unit is in fine adjustment mode LED display FINE On ns indicates current intensity setting On Off LUMEN DYNAMICS Fluorescence Illumination In Control 6 5 Display Format Reference 6 5 1 The formatting used on the display board is used to identify the unit of measurement watts Hours of the value Display Format Displays the iris set
62. umination In Control 7 Calibration Radiometry optional 711 All X Cite exacte units are capable of being calibrated with an X Cite Power Meter XR2100 or X Cite Radiometer XR2000 optional accessories Once calibrated the lamp output can be set in absolute units watts for easily repeatable illumination levels 7 1 2 For convenience the basic instructions for using the X Cite Power Meter XR2100 or X Cite Radiometer XR2000 with the X Cite exacte are provided here For full instructions and safety information refer to the complete Optical Power Measurement System User s Guide 035 00390R or Radiometer XR2000 User Guide 035 00285 7 1 3 Important Buttons and Settings underlined settings are recommended for use in microscopy RELATIVE ABSOLUTE toggles between readings in 96 relative and watts absolute POWER IRRAD toggles between readings in W power and W cm irradiance scale will adjust between W and mW automatically XR2000 only X CITE CAL initiates calibration sequence 7 2 X Cite exacte Power Reading using the XR2100 Power Meter or XR2000 oet the X Cite exacte to the desired intensity Close X Cite exacte shutter using SHUTTER button Remove light guide from microscope adapter onap the 3mm red light guide adapter into the top of XR2100 if not already in place o Loosen thumb screw on red light guide adapter fully insert and fasten light guide into holder 6 Turn XR2100 on using
63. warm up period has completed Wait until the warm up period has completed before using the unit to illuminate a specimen Lumen Dynamics recommends 20 minutes to ensure a stable output Note Unit should not be turned off unless the lamp has been on for a minimum of twenty minutes LUMEN DYNAMICS Fluorescence Illumination In Control 6 1 6 To power down the unit after use set the main power located on the front panel to 0 Note lf the lamp is turned off and an attempt is made to turn it back on before it has fully cooled the cool message will appear on the display The lamp wil automatically re strike when the lamp has cooled 6 2 Quick Start Illuminating a Specimen 6 2 1 When the X Cite exacte is first powered on the internal shutter will be in the closed position as a safety precaution and the iris will be in the 096 position 6 2 2 To illuminate a specimen press and release the SHUTTER button to open the shutter OPEN LED will turn on 6 2 3 Use UP DOWN buttons to adjust intensity to desired level See section 6 6 and 6 7 for additional information on intensity modes and adjustment LUMEN DYNAMICS PUTTING YOU IN CONTROL Fluorescence Illumination In Control 6 3 Front Panel Button Functions Button Condition Descipion _ SHUTTER Opens closes internal shutter Cycles between COARSE FINE and within 1 second HOURS mode Been andi Toggle be
64. x asp 2275 435 77 http www environmental expert com technology aspx 7idCategory 2054 amp word mercury 20spill 20kits http www alibaba com products spill_kit 4 html Warning Should this X Cite exacte unit be used in a manner not specified by Lumen Dynamics the protection provided by the equipment may be impaired LUMEN DYNAMICS Fluorescence Illumination In Control gt gt gt gt gt o Warning The method in which lamps are disposed of must comply with local rules and regulations for disposal of hazardous materials Lamps may be returned to Lumen Dynamics providing they are returned in their original packaging Lumen Dynamics will dispose of them in the appropriate manner Warning This unit is designed for bench top use only Always ensure that the unit is operated on a hard stable surface This will prevent obstruction of the bottom chassis ventilation openings Any obstruction of these openings could result in a possible over heating condition Do not attempt to remove or tamper with the rubber feet located on the bottom of the unit Caution The lamp module s operational life can be significantly shortened if it is handled incorrectly Do not touch the bulb s glass envelope or the inside surface of the reflector Skin oils can cause the lamp module to fail prematurely Caution Prior to opening the unit and handling the lamp module allow a minimum of 5 minutes for the lamp module to cool

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