TPersonal user manual
Contents
1. nesnunnnnnnnnnnanna suunan RRE 50 13 Declaration of Conformity ossna vassida ema k ne nei 51 4 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company E CIA E EH TE dn ie EE Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 1 Introduction 1 1 Field of Applications Today thermocycling is a standard technique in all molecular biology labs The new Biometra TPersonal Thermocycler is licensed for PCR applications research use only and intended to be used for performing polymerase chain reactions The instrument accounts for the increased demand for flexibility and prevents bottlenecks whenever a limited number of samples have to be amplified with different protocols Due to a new memory structure the programming of the TPersonal Thermocycler is now even easier and delivers highest ease of use to the customer 1 2 Special features 1 2 1 TPersonal block modules The TPersonal Thermocycler is available with three different block formats For standard applications a 48 well block for 0 2 mi single tubes strips of 8 tubes or 48 well microplates is offered For large volumes a 20 well block for 0 5 ml tubes is available The combi block offers maximal flexibility for the use of different sized tubes The combi block has a capacity for up to 48 x 0 2 ml tubes strips or plates or 18 x 0 5 mi tubes When using small cap tubes the capacity increases to 35 x 0 5 ml tubes for the c2. Note While lid preheating the block is held constantly at 25 0 C The program starts as soon as the lid has reached the set temperature During the preheating phase the display of the lid temperature alternates between the current lid temperature and preheating 5 6 4 View remaining run time Display during operation Prog 3 Biometra DIr 2 Step 1 Cycle 1 of 6 Temp 5 00 gt 62 0 C Time Oh4m0s gt Oh5m0s Lid 27 0G Dags System Seele Seg Edi To view remaining run time press Info Prog 3 Biometra Dat 2 Additional information about active pgm Status Step 1 Time Cycle 1 OF 56 Remaining time 43h34m peer The current remaining run time id displayed in line 4 5 6 5 Pause stop program Display during operation 34 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company Prog 3 Biometra Dir 2 step 1 Cycle 1 of 6 Temp 250 C gt 620 C Time Oh4m0s gt Oh5m0s Lid va i Hae System Ser Sep None To stop or pause a program press Start Stop Stop Pause Directory 2 Program No 3 Biometra are Pause Back Se oe Press Pause to pause block Press Stop to stop block Note The program pauses in the current state The word Pause is displayed and alternates with the elapsed time You can continue the run with Start Stop 5 7 System settings Open the main screen Prog Temp ze C Lid 2000 e ae System side E Stop Bee
3. r rxrnnnnnnnnnnnnnnnvnnnnnnennnnnnnennnnnnnnnnnnnnnennnnnnnernnnnnnennnnnnnennnnnnnennnn 9 2 1 Definition of gt une el 9 2 2 General Safely ng ler e anne aa ae 9 5 MENN 12 3 1 ETEN 12 3 2 Unpack and Ee 12 3 3 iS tel Elei a e ae le 12 34 CCN volage einer EE 13 3 5 Le EE EE au T 14 4 Operating elements aa 0 Ge os 15 4 1 INETPREISONA MOM VIEW ee ee een 15 4 2 UCI eu 16 4 3 The TPersonal control panel annae nnnnun nenne nenne name nennen 17 4 4 Hion Peromnance Smart Ld ESIL ee een 18 o OPEIN E 19 5 1 ne Personal diS OLA E 19 5 2 Navigation within the TPersonal software 19 5 3 ORONO E 19 5 3 1 Select directory eee 19 532 Select program 0 EE 20 9 0 9 Enter program NAME EE ee 21 5 3 4 Enter lid temperature neh EE REE 22 9 3 9 Select deselect lid ore beatng nennen 22 5 3 6 Enter temperature and time settmgs nennen ennne nenn 23 5 3 7 Set 01 0 i ot annette tunnetan ta ran kaetav vr keda ken saku anne hakanr eek sk ed ees knr ent ee anerkennen 24 5 3 8 Cool below ambient Temperature 25 5 3 9 DAN DO le E 25 5 4 ESI DIOG AM nenne een E E ee 26 Instruction Manual TPersonal 10 2009 3 Biometra An Analytik Jena Company 5 4 1 Delete DOGMA ES E 26 5 4 2 lg EE p OVO fs LAE EE ee 27 5 4 3 COPY OGM a MAMM mm ua mt Ka 28 5 4 4 Delete progra EN 30 5 5 Further programming OPNONS rasen rennen 31 SS Program time NETEMEN ann anne 31 5 5 2 Program TOUCH 97 77 0 nee ar 32 5 5 3 Adjust heating and cooling rates nnnnnnennnn
4. C Time tt Options gt l 95 0 Oli Sm Os 2 95 00 Oh 1m Os E SA Oh 1m Os 4 72 0 Oh Im 0s 2 24 Info Insert Del Save Pgm Enter Use cursor keys 4 to navigate in the spreadsheet Press repeatedly cursor key gt to open a spreadsheet extension for programming time and temperature increments Enter temperature and time for each step and confirm with Enter Hours minutes and seconds are separated by dot To enter for example 1 minute 30 seconds type 1 30 To enter 1 hour type 1 followed by two Set loop Enter the target step for a back loop in column lt The number of back loops total cycle number 1 is entered in column Confirm with Enter To program a pause enter zero Pause will be displayed after moving forward to the next step Temp C Time lt H Options gt 3z 55 0 Oh Im Os 4 12 0 Oh Im 0s 2 24 EE 18 04 Oh 5m Os 6 164 0 Pause Info Insert Del Save Pgm Enter To enter temperature or time increments or to alter the ramp rate open spreadsheet extension press repeatedly gt Instruction Manual TPersonal 10 2009 39 Biometra An Analytik Jena Company Main funct AT C dt s A s 1 lt 3 00 2 lt 3 00 SE 0 10 10 1 00 4 lt gt 00 IES iis see he Sa yeu Ben Baker gt To set a temperature or time increment enter the increment value in columns dT C temperature or dt s time Values have
5. If a program ends with a pause it has to be manually stopped by the user see section 5 6 5 Note Biometra recommends to cool samples at a temperature of 16 C rather than 4 C This prolongs the life span of the instrument 5 3 9 Save program Save program with Save Pgm The program data are written to the permanent memory Temp C Time de Options gt Se 550 Oh Im Os 4 12 0 Oh Im OS 2 24 of 12 0 Oh 5m Os 6 16 0 Pause Info Insert Del Save Pgm Enter Confirm changes with Yes The run time is calculated nh ee 2 3 BIOMETRA Save changes Cancel Yes No Se eo eee 2 3 BIOMETRA Number of steps 6 Run time 1h13m46 SEN GAGE Note The run time is calculated according to the block type Please be aware that different block types provide different ramping rates see section 1 3 Technical specifications Note Please note that time increments see section 5 5 1 and altered heating and cooling rates see section 5 5 3 have an influence on the overall run time Instruction Manual TPersonal 10 2009 25 Biometra An Analytik Jena Company Prog Temp EEG Lid 24 210 Im 6 System SIE MoE 9608 Edit Note To view the remaining runtime during a run press Info in the main menu 5 4 Edit programs Select program as described in section 5 3 1 and section 5 3 2 Note During operation of the cycler the active program can be viewed but not modified I
6. Biometra Do not attempt to operate this device if physical damage is present Please keep the original packing material for return shipment in case of service issues A I Attention A Please fill out and send back the warranty registration card This is important for you to claim full warranty 3 3 Installation Conditions e Place the TPersonal Thermocycler on a stable surface in a dry safe environment For details see working conditions in table Technical specifications see chapter 1 3 e Let equilibrate the TPersonal Thermocycler to room temperature before starting operation 1 to 6h e Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains e Make sure that the ventilation slots on the bottom and the rear are not obstructed Make sure that there is no object underneath the thermocycler that may block the ventilation slots at the bottom e g a piece of paper etc e There must be sufficient distance between the ventilation slots on the rear side of the Thermocycler and a wall or another instrument min 10 cm Ensure that both the rear and bottom ventilation slits of the rear and bottom of the instrument are unobstructed Insufficient ventilation can cause overheating of the instrument 12 Instruction Manual TPersonal 10 2009 An Analytik Je
7. General safety precautions for laboratory work must be observed when working with the TPersonal Thermocycler e The TPersonal does not produce a sound power level that could be hazardous for the user The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Rapid heating of the thermoblock can cause liguids to boil explosively Always wear safety goggles during operation Close the lid before starting a program Do not heat samples without having the lid locked securely Be aware that samples are reaching high temperatures Do not touch or open hot tubes or microplates because hot liguid may guickly spill out Do not touch the heated lid Use only suited plastic ware in the TPersonal thermocycler Tubes and plates must show good fit when placed in the thermoblock Only use tubes that are suited for high temperatures tight lids Instruction Manual TPersonal 10 2009 9 gt e gt eee eee gt gt gt GPP Biometra An Analytik Jena Company The TPersonal Thermocycler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise gualified technical personal Do not use the instrument when damages of the housing block cable or other parts are visible Prior to connecting the unit to the power source please ensure that the voltage
8. To change instrument settings press System 1 System Info 2 Signal 3 Language Sprache 4 Display contrast 5 Diagnostics Page NV Back Enter Scroll though the list with the cursor keys V Enter selected function with Enter 5 7 1 System info Select System info Press Enter Instruction Manual TPersonal 10 2009 35 Biometra An Analytik Jena Company TPersonal Serial No 123456 Blocktype Vers Biometra 2008 Back This page shows the serial number of the instrument and software version 5 7 2 Switch beep on off There is an option for an audible signal whenever a program enters a pause and when the program is finished If this option is active the beep can be switched off by pressing any key of the keyboard Select signal press Enter With signal Cancel 5 7 3 Select language The display of the TPersonal can be switched from English to German Select Language Sprache Press Enter Language Sprache German Deutsch Engir N Engli sed Cancel To confirm your selection press Enter 5 7 4 Display contrast The display contrast can be set in 4 levels to achieve optimum visibility under individual light conditions Select display contrast press Enter 36 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company Contrast 0 4 or arrows 2 lae s Cancel Enter User cursor keys to in decrease contrast value or directly enter setting The default setting
9. even temperature distribution in the tubes For instruments featuring the new lid design a maximum temperature of 99 0 C should can used en E een B 3 BIOMETRA Lid temp 99 0C Preheating auge Page V See cn Luts Om 5 3 5 Select deselect lid pre heating You can choose whether the lid is pre heated before the program starts This is to avoid evaporation during the initial heating phase Note During the preheating of the lid the block is held constant at 25 C ee eee a3 3 BIOMETRA Lid temo 99 00 Preheating Kats Page V Dave Pom 1 Enter Om Note The default setting which is recommended for most applications is preheating On After all pre settings have been made press Enter to open the programming spreadsheet 22 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 5 3 6 Enter temperature and time settings The programming spreadsheet Temp C Time D Options gt 1 4 PA ES 4 Taro Insert Del Save Pgm Enter Note In this spreadsheet you can enter all parameters for your cycling protocol In addition you can set special parameters like touch down ramping rates or time increments For details on these special parameters see section 5 4 1 cursor keys lt gt You can navigate in the spreadsheet without limitations with the four Note Each setting is confirmed with Enter The cursor moves automatically to the
10. program existing of letters numbers and symbols L 3 3 N ABCDEFGHIJKLM NOPORSTUVWXYZ C QO amp Blank S bel Name OK Enter SC lt gt Use cursor keys to select a letter NJ Accept letter with Enter O 3 3 BE N ABCDEFGHIJKLM NOPORSTUVWXYZ HC Okt SI Blank s bel Name OK Enter lt gt Use cursor keys to select next letter Accept letter with Enter Repeat until name is completed Note Press Del to delete a false letter You may also use certain special characters to name the folder To enter a blank press the Blank button Note Do not use special characters if you would like to control the TPersonal Thermocycler by the Thermocycler Manager Software Instruction Manual TPersonal 10 2009 21 Biometra An Analytik Jena Company ak EULERI SIND 3 BIOMETRA A ABCDEFGHIJKLM NOPORS TUVWXYZ AFO eres Blank S Beil Name OK Enter Once the name is completed confirm name with name OK oo B 3 BIOMETRA Lid temp IME Preheating On 5 3 4 Enter lid temperature You can enter a temperature for the heated lid in a range of 30 C to 99 C Note Thanks to a new lid design significantly lower lid temperatures than Info Page V save Pgm Ense inthe past can be used for thermocycling The lower lid temperature leads to a higher temperature uniformity within the lid and thus to a more
11. read the following return instructions in chapter 10 1 10 1 Instructions for return shipment In case of an instrument failure that cannot be fixed by the procedures described in section 7 please proceed as follows e Return only defective devices For technical problems which are not definitively recognisable as device faults please contact the Technical Service Department at Biometra Tel 49 551 50881 10 12 Fax 49 551 50881 11 e mail service biometra com e Please contact our service department for providing a return authorization number RAN This number has to be applied clearly visible to the outer box Returns without the RAN will be not be accepted e Important Carefully clean all parts of the instrument of biologically dangerous chemical or radioactive contaminants If an instrument is contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible losses resulting from insufficient decontamination of the device e Please prepare written confirmation that the device is free from biologically dangerous and radioactive contaminants The declaration of decontamination see section 11 must be attached to the outside of the packaging e Use the original packing material If not available contact Biometra or your local distributor e Label the outside of the box with CAUTION SENSITIVE ELECTRONIC INSTRUMENT e Please enclose a note which contains
12. to be entered with a minus sign if temperature or time shall be reduced step by step Confirm with Enter Note Temperature or time increments have to be set within a loop otherwise they will be without effect gt To alter the ramp rate enter new ramping temperature in column 7 s gt Save program with Save Pgm Start program Press Start Stop the main screen Select directory and program with cursor keys or enter program number Optional To control program prior to start press View and use Page VA or the cursor keys and 4 to navigate blockwise or stepwise within the spreadsheet Start selected program with Start Note After the High Performance Lid has reached the programmed temperature there is a 40 second eguilibration phase before the program starts preheating mode on Display during operation 0 000 Prog 3 Biometra Dit 2 Step 1 Cycle 1 2 amp Temp 250 gt 62 0 C Time Lid Preheating 55 0 C 100 E System SEA EAS SS Ber Note When the preheating function is activated the block is held constantly at 25 0 C when the block is heated up During the preheating phase the display of the lid temperature alternates between the current lid temperature and preheating gt Press Info for remaining run time Press Exit to leave the info screen To stop pause active program press Start Stop Stop Pause Directory 2 Program No 3 Biometra LMI Pause Back Of Pres
13. 0 1 00 2 00 3 CT 4 Page NV Dire ee Back Enter Select directory with cursor keys M V or enter directory number and press Enter 0 2 Page N Edit program Page V BTOMETI BIOMETI BIOMETI BERND2 BIOMETI VIEW TATA pm Im IT m WN M EE Select program with cursor keys or enter program number Note To be sure that the correct program is selected it can be displayed by pressing View see section 5 6 2 Press Start to run program 5 6 2 View program prior to start Page N EL 2 Edit program Page V BIOMETI BIOMETI BIOMETI BERND2 BIOMETI View Im ME MI SD E Im See ee To check a program prior to start it can be displayed by pressing View 0 2 Page V 3 BERND2 Lid temp Preheating Back 99 Se emale DE OFF Scroll through the program with Page NN and Page V Instruction Manual TPersonal 10 2009 33 Biometra An Analytik Jena Company Temp C Time Options gt 1 3 95 0 Oh 5m Os Sc 25 0 Oh lm Os SE 5540 Oh lm Os 4 7250 Oh Im OS 2 24 Page TV Back Sr uate To run program press Start 5 6 3 Display during operation During preheating of the lid the following screen is displayed Prog 3 Biometra Dir 2 Step 1 Cycle L of 6 Temp 2550 gt 62 0 C Time Lid Preheating 55 0 C no System See See Eda
14. Biometra An Analytik Jena Company Personal Thermocycler Instruction Manual Ver 09 10 Kroer Model Order No TPersonal 20 050 550 TPersonal 48 050 551 TPersonal Combi 050 552 Please read these instructions carefully before using this apparatus Biometra GmbH Rudolf Wissell Str 30 Service Department D 37079 G ttingen Rudolf Wissell Strasse 14 16 Tel 49 0 551 50 68 6 0 D 37079 G ttingen Fax 49 0 551 50 68 6 66 Tel 49 0 551 50 68 6 10 or 12 email Info biometra com Fax 49 0 551 50 68 6 11 Internet http www biometra com email Service biometra com Biometra An Analytik Jena Company This document describes the state at the time of publishing It needs not necessarily agree with future versions Subject to change 2 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company Contents C WAU OQUCUIOI EE OE 6 1 1 FEOLA el lee ee ee ee ee 6 1 2 SPECIAL SALES ee ee re EE PN 6 1 2 1 TPersonal block modules nene nennen nennn nenne nn name naat 6 1 2 2 TPersonal heating and cooling rates nnveneennnnnennnanennnanennnnane anna 6 1 2 3 Personal operating software 6 1 3 Technical specifications TPersonal Thermocvcer nne 7 1 4 EENet ee 8 1 4 1 PCR License Legal Disclaimer annan en nennn annan 8 1 4 2 Soola ee NE EE EN 8 1 4 3 HEDIN mederne 8 1 4 4 Meaning of the Instruchons nene nnenennene annan nnenena 8 2 Safety and Warning Notices
15. Oh 5m Os 2 4 PAEO Insert Del Save Pgm Enter Repeat for all temperature steps in the program 5 3 7 Set loop In general loops are defined by two parameters 1 The target for the back loop row lt and 2 The number of back loops row 4 Temp C Time Pai tt Options gt Le 15 0 Oh 5m Os E 9550 Oh Im Os 3 SH Oh Im Os 4 12 0 Oh lm Os TC Insert Del Save Pgm Enter In the row labelled with enter the target step for a back loop and press Enter Temp C Time CE Options gt 11 Del Oh 5m Os an Bl Oh Im Os ES Se Oh Im Os 4 NG Oh Im Os 2 Taro Insert Del Save Dom Enter In the row labelled with you can enter the number of back loops Note total cycler number n back loops 1 e g enter 29 for a total number of 30 cycles Temp C Time lt OpEtons gt 1 95 0 Oh 5m Os 2 95 0 Oh Im Os a 530 Oh 1m Os 4 12 0 Oh Im OS 2 24 AE Insert Del Save Pgm Enter This protocol now contains 25 cycles between step 2 95 0 C for 1 minute and step 3 55 0 C for 1 minute 24 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 5 3 8 Cool below ambient temperature Temp C Time Options gt ER cem Oh Im Os 4 12 0 Oh Im OS 2 24 Si FE Oh 5m Os 6 1 640 Pause Info Insert Del Save Pgm Enter To hold the temperature for an indefinite time enter 0 After pressing Enter pause is displayed Note
16. S A 40516 D Arp he JNE Power switch Context sensitive Keypad menu buttons Cursor keys yP Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 4 4 High Performance Smart Lid HPSL To achieve optimum pressure on the tubes the TPersonal is eguipped with a height adjustable heated lid Close the lid After the samples have been placed in the block close the lid Turn the wheel clockwise until you hear a clicking noise In this mode the pressure will not increase further even when you keep on turning the wheel Note The pressure of the lid has been optimized for a fully loaded block If only very few tubes are loaded to the block you should place dummy tubes in the four corner positions to avoid damage of tubes by excessive pressure OO OO OOO 9 2022 DO OO G J O O F O g 00000000 DL SOLL CC OO OO Open the lid First Release pressure by turning the wheel counter clockwise As soon as there is no more resistance the pressure has been released Then Open the lid Important The lid should not be opened under pressure because this leads to damage of the locking mechanism 18 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 5 Operating 5 1 The TPersonal display The TPersonal display provides information about the instrument and about the status of the active program Prog Temp 2550 Deckel 2200 ats System Sole oja Bees The function of
17. d turn wheel carefully This pin overcomes the automatic clutch mechanism Thus care must be taken not to apply excessive pressure 42 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company Lid arrested in upper position carefully turn wheel with pressed pin clockwise until you feel normal resistance no more clicking noise clutch is released Release pin and turn lid down until the clutch mechanism is activated clicking noise optimum pressure applied Lid arrested in down position carefully turn wheel with pressed pin counter clockwise until you feel normal resistance no more clicking noise clutch is released Release pin and turn wheel counter clockwise until pressure is completely released Open lid Important When the clutch mechanism is active optimum pressure is applied Do not use pin to further increase pressure This may lead to damage of tubes and instrument Instruction Manual TPersonal 10 2009 43 Biometra An Analytik Jena Company 8 Maintenance and repair 8 1 Cleaning and Maintenance The TPersonal was built to operate for a long time without the need for periodical maintenance Nevertheless occasionally cleaning of the air inlet may be necessary to maintain the efficiency of the Thermocycler Insufficient airflow may lead to reduced heating and cooling rates see chapter 7 1 The inlet for the airflow is located at the bottom side of the instrument Be sure that the inl
18. ege 34 BAG s i iii aia aa i 29 EN VEN EEE AADA 32 33 SLOP EEE 34 time increment o nennnennennannnnenennnnnnne 31 TOUGIN COW EE 32 REMAINING run Ume 34 Kee 44 Safety precautions ccccceesseeeeeeeeeeeees 9 SE EE 14 SOIUIE NO ee ee 44 Short manual 38 Subject Index 53 SEE 35 Syst m Settings de 35 Technical specifications 7 Trouble shooting 42 Warranty eronrrnrnnrernnrronnnnrnrnnrnrnnernnrnennnsnn 52 53
19. eps Note The insertion and deletion of steps may have an influence on existing loops Be sure that all settings are updated to maintain a correct cycle 5 4 3 Copy program Select the program you want to copy as described in section 5 3 1 and section 5 3 2 St oo G Test 5 1 TEST Edit program 5 2 BIOMETI 3 Boks 5 4 BIOMET3 Page N Page V Goo DE meite Press Copy Del E Oe oot BIOMET2 Copy Pom Delete Dom Back To copy this program press Copy Pom 28 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company EI AC Page NV Back Target Enter Select the subdirectory with the cursor keys or enter number on the keypad 710 Target 00 1 5 2 00 3 CT 4 Info Page NV Back Enter Press Enter loo 0 empty 1 empty Target EB 2 empty 3 empty 3 4 empty Page M Page V Back Edit Select program number for the program copy and confirm with Enter COPY EE ed 3 3 BIOMET2 To 1 2 od Si 3 empty BaCk Com Press Copy Copy E yo 3 BIOMET2 To 2 poo 3 empty gt EE EE E EE 7 2 0 empty 3 1 empty Edit program 5 2 empty 3 BIOMET2 5 4 empty Page M Page V Copy Del Edie Instruction Manual TPersonal 10 2009 29 Biometra An Analytik Jena Company N
20. et is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dust can be removed easily from the inlet with a conventional vacuum cleaner Additionally the Thermocycler housing may be cleaned from time to time with a smooth cotton cloth Do not use strong detergents or organic solvents for cleaning Never treat silver block with abrasive agents Important Appropriate safety regulations must be observed when working with infectious or pathogenic material 8 2 Servicing and repair The TPersonal Thermocycler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise gualified technical personal The Service department offers Thermocycler maintenances and temperature verifications Biometra recommends an annual maintenance and a biannual temperature check for all Thermocyclers Please call the following phone number for detailed information 49 551 50881 10 12 8 3 Firmware update For instruction for firmware upgrade please contact the Biometra Service Department or your local distributor sales representative 6 4 Replacement of Spare Parts Only original spare parts mentioned in these operating instructions are allowed 44 Instruction Manual TPersonal 10 2009 9 Accessories 9 1 Plasticware Biometra An Analytik Jena Company Biometra o
21. f you want to change settings of the active program for further experiments you have to save a Copy of this program to another memory For further information about copying programs see section 5 4 3 5 4 1 Delete program steps Open the programming spreadsheet Temp C Time lt OPCIONS gt 1 95 0 Oh 5m Os 23 Doe Oh Im Os Sr 5 Oh 1m Os 4 FE Oh Im OS 2 24 JA EG issues Del Save Dom Enter To delete a program step press Insert Del Insert Delete step 1 95 0 Oh 5m Os Zn el Oh lm Os Cie Sech Oh lm Os 4 T20 Oh Im OS 2 24 Del Cancel Delete Insert Enter the number of the step that you want to delete and press Delete Example To delete step 3 enter 3 and press Delete Insert Delete step 3 ie En Oh 5m Os 990 Oh lm Os Sr Sie Oh lm Os 4 1450 Oh lm OS 2 24 lt bel sees Del ete Insert 26 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company Insert Delete step B Li 1540 Oh 5m Os 2 15 0 Oh Im Os J 9 10 On Im Os 4 Tae Oh Im OS 2 24 gt gt gt Deleting step 3 lt lt lt The corresponding step is deleted Temp C Time g Options gt L 150 Oh 5m Os as 95 0 Oh Im Os ES 120 Oh Im OS 2 24 4 12 0 Oh 5m Os Info Insert Del Save Pym Enter Note The insertion or deletion of steps may have an influence on loops If for a deleted step a loop was defined the loop will be deleted too Please ins
22. ffers a broad range of plasticware for the use in PCR The following table provides an overview for the product portfolio Cat no 050 310 050 320 050 254 050 255 050 225 050 232 050 213 050 253 050 231 050 240 050 237 050 256 050 257 050 236 050 194 050 694 050 251 050 252 Instruction Manual TPersonal 10 2009 Description Single tubes 0 2 ml tubes with caps 0 5 ml tubes with caps Tube strips Strips 8 tubes and flat caps Strips 8 tubes and domed caps 48 well plates 48 well microplate 96 well plates 96 well skirted 96 well non skirted low profile 96 well non skirted 384 well plates HSQ 384 well skirted 384 well microplate Miscellaneous Silcone mat Adhesive film Heat Sealing Film Heat Sealing Film Aluminum 96 well aluminium plate 384 well aluminium plate Nop mat 96 well Nop mat 384 well Quantity 1000 pcs 1000 pcs 125 pcs 125 pcs 50 pcs 25 pcs 25 PCs 25 PCs 50 pcs 50 pcs 50 pcs 100 pcs 100 pcs 10 pcs 1 pcs 1 pcs 20 pcs 20 pcs 45 Biometra An Analytik Jena Company 10 Service Should you have any problems with this unit please contact our service department or your local Biometra dealer Biometra GmbH Service Department Rudolf Wissell StraBe 14 16 D 37079 G ttingen Phone 49 0 5 51 50686 100r 12 Fax 49 0 5 51 50686 11 e mail Service biometra com A If you would like to send the unit back to us please
23. for use within certain limits of voltage 73 23 EEC und and im Sinne der EG Richtlinie f r die elektromagnetische Vertr glichkeit 89 336 EWG following the EC directive about the electromagnetic compatibility 89 336 EEC Hiermit erkl ren wir da folgende Thermocycler Herewith we declare that the following Thermocyclers Typen types TPersonal 20 TPersonal 48 TPersonal combi Best Nr Order No 050 720 050 723 050 724 den grundlegenden Anforderungen der corresponds to the basic reguirements of EG Niederspannungsrichtlinie 73 23 EWG und der EC low voltage directive 73 23 EEC and the EG Richtlinie ber die elektromagnetische Vertr glichkeit 89 336 EWG entsprechen EC directive about the electromagnetic compatibility 89 336 EEC Folgende harmonisierte Normen wurden angewandt The following harmonized standards have been used EN 55011 1998 A1 1999 A2 2000 EN 55022 1998 A1 2000 A2 2003 EN 61000 3 2 2000 EN 61000 3 3 1995 A1 2001 EN 61000 6 1 2001 EN 61010 1 2001 EN 61010 2 010 1994 A1 1996 Dr J rgen Otte Quality Manager Instruction Manual TPersonal 10 2009 51 Biometra An Analytik Jena Company 14 Warranty This Biometra instrument has been carefully build inspected and quality controlled before dispatch Hereby Biometra warrants that this instrument conforms to the specifications given in this manual This warranty covers defects in materials or workmanship as described under the follo
24. ger of overheating Let eguilibrate the TPersonal Thermocycler to room temperature before starting operation There must be sufficient distance between the ventilation slots on the rear side of the Thermocycler and a wall or another instrument min 10 cm Danger of overheating Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company This instrument is designed and certified to meet EN 61010 1 safety standards It should not be modified or altered in any way Alteration of this instrument will void the warranty void the EN61010 1 certification and create a potential satety hazard Place the TPersonal Thermocycler on a stable non flammable surface in a dry safe environment For details see working conditions in table Technical specifications see chapter 1 3 Do not use alcohol e g methanol ethanol organic solvents or abrasives to clean the instrument For transports always use the original Biometra box Instruction Manual TPersonal 10 2009 11 Biometra An Analytik Jena Company 3 Installation 3 1 Content of delivery Thermocycler Mains connector Manual Short Manual St nt nt Dt 4 2 3 4 Please keep the original packaging material for return shipment in case of servicing The TPersonal shipping box provides a specially developed system for contact free transport of this electronic device 3 2 Unpack and Check Unpack and carefully examine the instrument Report any damage to
25. he EEA EEA European Economics Area comprising all EU members plus Norway Iceland and Liechtenstein Dieses Symbol die durchgestrichene Abfalltonne bedeutet dass dieses Produkt von der Firma Biometra fur eine kostenlose Entsorgung zuruckgenommen wird Dies gilt nur fur Gerate die innerhalb Deutschlands gekauft worden sind Kontaktieren Sie fur die Entsorgung bitte die Biometra Service Abteilung AuBerhalb Deutschlands wenden Sie sich bitte an den lokalen Handler Dieses Symbol gilt nur in Staaten des EWR EWR Europaischer Wirtschaftsraum umfasst die EU Mitgliedsstaaten sowie Norwegen Island und Liechtenstein Cet symbol conteneur a d chets barre d une croix signifie que le produit en fin de vie doit tre retourne a un des systemes de collecte mis a la disposition des utilisateurs finaux en consequence des regulations par la loi de votre pays Pour des information additionel nous Vous demandons de contacter votre distributeur Cet symbole s applique uniquement aux pays de EEE EEE Espace conomique europ en qui regroupe les Etats membres de I UE et la Norv ge Islande et le Liechtenstein 50 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 13 Declaration of Conformity G ttingen den 20 08 2004 im Sinne der EG Richtlinie ber elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 73 23 EWG following the EC directive about electrical equipment
26. ional data transfer port for in tube sensor programs each max 99 steps program max 99 cycles Software 0 Program steps are easily entered in a spread sheet Options time increment temperature increment set ramping rate Autorestart function 50 60 Hz Fuses seelabelonthebackoftheinstrumen 5 C 35 C max 70 humidity max 2000 m NN 6 7 kg _ measured inside the block Capacity increases to 35 x 0 5 mi tubes by use of small cap tubes Noise emission very low Instruction Manual TPersonal 10 2009 7 Biometra An Analytik Jena Company 1 4 Legal Notes 1 4 1 PCR License Legal Disclaimer Purchase of a Biometra Thermocycler conveys a limited non transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under one or more of US Patents Nos 5 038 852 5 656 493 5 333 675 5 475 610 and 6 703 236 or corresponding claims in their non US counterparts owned by Applera Corporation No right is conveyed expressly by implication or by estoppel under any patent claim reagents kits or methods such as 5 nuclease methods or under any other apparatus or system claim including but not limited to US Patent No 6 814 934 and its non US counterparts which describe and claim thermal cyclers capable of real time detection Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Bio
27. is 2 which meets average conditions 5 7 5 Diagnostics The diagnostic screen contains internal information used by the Biometra service department for servicing 37 Instruction Manual TPersonal 10 2009 6 Short ma Safety warnings Biometra An Analytik Jena Company nual Do not open the instrument unless you are authorised to do so Check the label at the backside if the instrument has correct voltage configuration 100V 115 V or 230 V The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Rapid heating of the thermoblock can cause liquids to boil explosively Always wear safety goggles during operation Close the lid before starting a program It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes If you still decide to use oil do not use silicon oil Mineral oil may be used Ensure that both the rear and bottom ventilation slits at the rear and bottom of the instrument are unobstructed Insufficient ventilaton can cause overheating of the instrument Tip At regularly intervals clean the bottom s ventilation slits from dust to ensure maximum venting capability The TPersonal control panel Display Baron al Tharmocyvrclar biometra gt menu buttons TPersonal mai
28. m the inlet with a conventional vacuum cleaner 7 2 Restart due to unrecognised power failure High voltage fluctuation can lead to an automatic restart of the thermocycler In this case the cycler restarts at the step where there power failure has occurred To avoid voltage fluctuation do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a centrifuge 7 3 Adaptation of protocols from other cyclers Since the TPersonal is a fast instrument it may be necessary to reduce the heating and cooling ramps to run protocols from other cyclers For the setting of the heating and cooling ramps see section 5 5 3 Alternatively the time settings may be extended 7 4 Lid can not be opened The lid of the TPersonal is equipped with a snap mechanism that arrests when the lid is pressed down When itis pressed down again the snap mechanism unlocks and the lid can be opened As soon as the heated lid has been lowered by using the lid wheel the lid can not be opened and first the pressure has to be taken from heated lid Turn the lid wheel counter clockwise to relieve the pressure see section 4 4 and open the lid 75 Releasing wheel in case of blocked lid Note When the lid is in the extreme up or down position it may happen that the wheel is uncoupled In this situation the clutch mechanism is active in both directions clicking noise in either direction To unlock wheel press down metal pin with a ball pen an
29. n Voltage of 100 or 115 Volt use two slow blow fuses of 4 Ampere 2x 4ATT 4 Reinsert the fuse holder 5 Switch on the instrument Instruction Manual TPersonal 10 2009 13 3 5 Initial self test Biometra An Analytik Jena Company After switching on the TPersonal the serial number of the instrument and the software version is displayed Vers T Personal Serial number Block type C biometra 2008 The TPersonal then checks all programs in all subdirectories so called RAM check Checking directory 7 program 4 T Personal Serial number Block type Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 4 Operating elements 4 1 The TPersonal front view Lid wheel for adjustment of lid pressure Lid pressure lock Keypad Display Context sensitive Instruction Manual TPersonal 10 2009 15 Biometra An Analytik Jena Company 4 2 The TPersonal rear view Easy to clean operating surface Ay O ae K x Al Power cord plug Fuse holder for 2 fuses for specification of fuses see type label on the instruments backside 16 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 4 3 The TPersonal control panel Easy to clean operating surface Display biometra Kess Thermocycler VC S
30. n screen Prog Temp AG FE CC Lid 24 0 C Into System Seele SECIS Edie Softkey 1 Softkey 2 Softkey 3 Softkey 4 The function of the four soft keys beneath the display is context sensitive The respective function is shown in the display The function of each key can differ from screen to screen Create modify and save programs Press Edit to enter the edit mode gt Select a user directory with the cursor keys gt tt gt Press Enter gt Select a program with cursor keys or by number gt Press Edit 38 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company Enter program name 0 3 3 A ABCDEFGHIJKLM NOPORS TUVWXYZ C O amp Blank S Bel Name OK Enter gt Select letter with cursor keys and accept with Enter Accept name with Name OK Enter lid temperature el PE 3 3 BIOMETRA Lid temp 99 0C Preheating On IE ane Page 1 paws E gm Enter gt Set lid temperature for the Biometra High Performance Lid a few degrees higher than the highest block temperature programmed For most applications like PCR a lid temperature of 99 0 C is recommended Confirm with Enter Accept preheating mode on gt block starts after the lid has reached the programmed temperature Note Prior to block start there is a 40 sec temperature equilibration Press Enter The programming spreadsheet Temp
31. na Company e Make sure that the main supply voltage is in accordance with the label above the power connection see section 3 4 e Connect the TPersonal Thermocycler to a grounded socket Prior to connecting the unit to the power source please ensure that the voltage selector at the back side of the instrument is set to the required voltage Danger of electric shock Unplug the power cable before you open the TPersonal Thermocycler e he display contrast can be adjusted to local light conditions 5 7 4 3 4 Operation Voltage The TPersonal Thermocycler can operate at 100 115 or 230 Volt Important Prior to connecting the TPersonal to the mains make sure that the setting of the Voltage selector at the bottom of the instrument is in accordance with your mains Voltage and that it is equipped with the correct fuses Instrument bottom Instrument backside Voltage indicator Mains plug 4 Fuse holder 1 To change the operation Voltage settings switch off the instrument and disconnect the mains plug 2 Use a coin or another round shape item to turn the adjustment slot of the Voltage selector at the bottom side of the instrument see picture 1 to the new Voltage setting 3 Pull out the fuse holder at the backside of the TPersonal see picture 2 Load the fuse holder with two fuses corresponding to the designated operation Voltage At an operation Voltage of 230V use two slow blow fuses of 2 Ampere 2x 2ATT at an operatio
32. next field Alternatively you can confirm a value by moving forward with the cursor keys Note At any time you can activate the help function with Info Note In an existing protocol program steps can be deleted and inserted For further information on deleting and inserting program steps see sections 5 4 1 and 5 4 2 Now enter temperature for the first step and press Enter In the next row you can enter the time for this temperature Temp C Time lt Options gt 14 2940 SCH ES 4 Info ke See Dei Save Dom Enter Note There is a convention on how time settings are entered in all BIOMETRA cyclers hours amp minutes amp seconds If you enter a number without dot this value will be interpreted as seconds 300 gt 5 minutes To program minutes enter a after the number of minutes To enter hours enter after the number You can also enter any combination of hours minutes and seconds Example for 1 hour 30 minutes 20 seconds enter 10 30 20 The time values will be displayed in the following format Oh 00 m 00s Note To program a pause press 0 and Enter For the corresponding step Pause will be displayed To manually pause a program see section 5 3 8 Instruction Manual TPersonal 10 2009 23 Biometra An Analytik Jena Company Enter 5 dot for 5 minutes initial denaturation Press Enter Temp C Time Options gt he SE
33. nn nennen 32 5 6 FUN OLO AN E 32 5 6 1 Select and starn 8 910 4 2 10 ME 32 5 6 2 VIEW DEOGLAM Prior to E a 33 5 6 3 Display during operaton nenne nnennnennenne nennen 34 5 6 4 View remaining run TING een 34 5 6 5 Pause SIOD DIOTAN airera ee een 34 Dr SS FN SS ln Le else ee een 35 5 7 1 SEI anregen 35 5 7 2 MENA r e 36 5 7 3 SEIECL ele E 36 5 7 4 BIS SEV CONTEST een aiamaad arena nern he are 36 5 7 5 Be SEE EN EE NE 37 ET AEA IA eace cece nema ceevaesccus aE 38 TOER 42 7 1 Slow heating and cooling rrrannrvrnnnrnvnnnrrnrnnnrnrnnnrnnnnnrennnnnsnnnnnennnnnsennnnesnnnanennnnnsennnnsene 42 7 2 Restart due to unrecognised power Tailure 42 7 3 Adaptation of protocols from other cyclers naa 42 7 4 Bie ola DOLDE OPEN 2 DE 42 7 5 Releasing wheel in case of blocked lid nnvenennnenennnnane nn vann nnnenn anna 42 8 Maintenance and Rn E 44 8 1 Cleaning and Mamtenance nene nennen nenne nnnnnnennnnnen nennen 44 EG SAMAD And TEDA EEE ee ee 44 8 3 Firmware update avane nnnnnennnnane nennen nnnnnnnnennnnnennnennennn nennen 44 8 4 Replacement of Spare Parts nennen ennennnn nenn 44 9 ZRECESSOFIES re 45 9 1 LO a ee UUE eae 45 10 SUE 46 101 Instructions TOF return SNPMEN nennen 46 10 2 Packing of the Thermocvcler 47 11 Equipment Decontamination Certificate rrannnxnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennnnnnnennnnnnnennnnnnnr 48 12 Note for the disposal of electric electronic waste
34. od of cleaning decontamination The equipment has been cleaned and decontaminated NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER 48 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company General Information for Decontamination Please contact your responsible health 8 safety officer for details Use of radioactive substances Please contact your responsible person for details Use of genetically change organism or parts of those Please contact your responsible person for details Instruction Manual TPersonal 10 2009 49 Biometra An Analytik Jena Company 12 Note for the disposal of electric electronic waste Note for disposal of electric electronic waste Hinweis f r die Entsorgung von Elektroaltger ten du traitement des dechets des appareils electrigue lectronique This symbol the crossed out wheelie bin means that this product should be brought to the return and or Renseignement separate systems available to end users according to yours country regulations when this product has reached the end of its lifetime For details please contact your local distributor This symbol applies only to the countries within t
35. ombi block 1 2 2 TPersonal heating and cooling rates The TPersonal was developed to meet highest demands The resultis a thermal cycler that reaches the highest heating and cooling rates in the class of personal thermal cyclers and excellent temperature uniformity The high heating and cooling rates ensure specific reaction conditions and the superior temperature uniformity guarantees that the reaction conditions are identical in every position of the block 1 2 3 TPersonal operating software The TPersonal operating software has been redesigned to ensure easy user management and clear menu guidance At the same time the instrument was equipped with a new front panel for faster navigation through the user menu The clear user interface in conjunction with the large screen makes TPersonal Thermocycler an intuitive to operate system 6 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 1 3 Technical specifications TPersonal Thermocycler Ordernumber 050 550 050 551 050 552 Model TPersonal 20 TPersonal 48 TPersonal combi 18 x 0 5 ml tubes 48 x 0 2 ml tubes 48 x 0 2 ml tubes stripes stripes 48 well microplates 48 well microplates 30 C 99 C 20 x 0 5 ml Block capacity EES viewing area 124 mm x 34 mm resolution 256 x 64 dots High Precision Smart Lid for optimum lid pressure and excellent temperature uniformity temperature range 30 0 99 0 C ports oo serial RS232 bi direct
36. ote If the program is copied to an already used storage space the existing program will be overwritten 5 4 4 Delete program Select the program you want to delete as described in section 5 3 1 and section 5 3 2 SL po Oo B 0 Weer 1 TEST2 Edit program 5 2 BIOMETI 3 BIOMET2 4 BIOMET3 Page N Page V Copy bel Edit Press Copy Del qs oo 3 BIOMET2 Copy Pom Delete Pgm Back To delete this program press Delete pgm E poo oo 3 BIOMET2 Delete program Cancel Delete Note Once a program has been deleted it cannot be restored Press Delete EI on HS 3 BIOMET2 2 gt Deleting proge ma In the directory structure the program is displayed as empty SE Erreger e TESTA 1 TEST2 Edit program 5 2 BIOMETI B 3 empty 4 BIOMET3 Page N Page V Copy Del Edit 30 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company You can enter a new program To get back to the main screen 2 x press cursor key 5 5 Further programming options Open the programming spreadsheet Temp C Time lt Options gt L 95 20 Oh 5m Os 2 ve Oh Im Os SEH Joa Oh 1m Os 4 Ta Oh Im 08 2 24 TAS Insert Del Save Pgm Enter Note To program special parameters you can scroll the display to the right Move the cursor to the right side row of the display Op
37. s Pause to pause block gt Press Stop to stop block Note The program pauses in the current state The word Pause is displayed and alternates with the elapsed time You can continue the run with Start Stop System settings Press System in the TPersonal main screen 40 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company System Info Signal Language Sprache Display contrast Diagnostics Page NV Back Enter 1 A W KO IM gt Select topic by using Page VA or the cursor keys f and U and press Enter 1 System Info Shows information on the serial number of the instrument and the software version 2 Signal Switch beep on off 3 Language Sprache The display of the TPersonal can be switched from English to German 4 Display contrast Adapt contrast to the local light conditions Diagnostics The diagnostic screen contains internal information used by the Biometra service department for servicing Instruction Manual TPersonal 10 2009 41 Biometra An Analytik Jena Company 7 Trouble shooting 7 1 Slow heating and cooling The TPersonal is equipped with a strong ventilator for the cooling of the heat sink The inlet of this fan is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dust can be removed easily fro
38. selector at the bottom of the instrument is set to the reguired voltage Make sure that the main supply voltage is in accordance with the label above the power connection see section 4 2 Unplug the power cable before you open the TPersonal Thermocycler Danger of electric shock Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains Connect the TPersonal Thermocycler to a grounded socket When only few samples are putin the block place additional tubes in the four corner positions This is to evenly distribute the lid pressure and prevents single tubes from excessive pressure Use of few tubes may result in damage of the tubes by excessive pressure Appropriate safety regulations must be observed when working with infectious pathogenic or radioactive material Ask the responsible local safety inspector for details The TPersonal Thermocycler must not be used with explosive flammable or volatile liquids Do not place fingers between lid and housing when opening or closing the lid Before opening of the lid release lid pressure It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes If you still decide to use oil do not use silicon oil Mineral oil may be used Ensure that both the rear and bottom ventilation slits not clogged by dust or other material Dan
39. systems 850 Lincoln Centre Drive Foster City California 94404 USA Biometra biomedizinische Analytik GmbH Rudolf Wissell Str 30 37079 Goettingen Germany icensad and authorized 1 4 2 Copyright All rights reserved It is not allowed to copy and publish the manual or parts of it in any form as copies micro film or other methods without a written authorisation from Biometra Biometra is pointing out that applied company and brand names are usually protected trade marks 1 4 3 Liability Biometra is not liable for damages and injuries caused by use not considering these operating instructions in parts or completely 1 4 4 Meaning of the Instructions Biometra recommends that you first read these instructions carefully This operation instruction is part of the product and should be kept over the full life time of the instrument It should also be forwarded to subsequent owners and users Make sure that additions and updates are inserted into the operation instructions 8 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 2 Safety and Warning Notices 2 1 Definition of Symbols Symbol Definition A Caution Refer to instruction manual Danger High voltage A AN Danger Hot surface 2 2 General Safety Instructions Please read this manual carefully before starting operation of the TPersonal Thermocycler The TPersonal Thermocycler is intended for sample incubation at varying temperatures e
40. the following a Sender s name and address b Name of a contact person for further inquiries with telephone number c Description of the fault which also reveals during which procedures the fault occurred if possible 46 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company 10 2 Packing of the Thermocycler Biometra uses an extra designed packaging system where the instrument is mounted in between two tearproof foils The Thermocycler is put onto the lower inlet and is fixed in between the foils by pressing the upper inlet down Note The Thermocycler is only protected from transport damage if the packing instructions are followed and the instrument is mounted in between the foils Biometra will not be responsible for transport damage by improper packing Folien schwebend Ger t zwischen den einspannen Mount instrument floating in between the foils Instruction Manual TPersonal 10 2009 47 Biometra An Analytik Jena Company 11 Equipment Decontamination Certificate To enable us to comply with german law i e 871 StrISchV 817 GefStoffV and 819 ChemG and to avoid exposure to hazardous materials during handling or repair please complete this form prior to the equipment leaving your laboratory COMPANY INSTITUTE ADDRESS PHONE NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluation Start Date Finish Date Hazardous materials used with this equipment Meth
41. the four soft keys beneath the display is context sensitive The respective function is shown in the display see also chapter 4 3 The function of each key can differ from screen to Screen 5 2 Navigation within the TPersonal software The TPersonal provides spread sheet programming This means that all program parameters are entered into a simple central spreadsheet Four navigation keys provide easy navigation within the software The back and the forward key have additional functions as described below The right cursor key moves the cursor to the next field lt gt This cursor can also be used to complete data entry By pressing the right NZ cursor settings will be saved and the cursor moves to the next field In the file directory this key moves to cursor forward to the next lower level IN The left cursor key moves the cursor back to the previous field lt gt In most screens this cursor is equivalent to the back softkey In the file directory this key moves the cursor back to the higher level 5 3 Create program Prior to writing a new program a program store has to be chosen The TPersonal provides 10 directories of which each can store up to 99 different programs For easy identification of the selected storage place the directories can be named see section 5 3 3 The total memory capacity is 1 500 program steps 5 3 1 Select directory Start with the main screen Instruction Manual TPersonal 10 2009 19 Biome
42. thout an effect 5 5 2 Program touch down For some applications it is useful to start with a higher temperature and to decrease this temperature from cycle to cycle This subsequent lowering of a temperature is called touch down To decrease the temperature from cycle to cycle enter a negative temperature increment in the row labelled with dT s Main funct AT C dt s A s Le 5500 2 3 00 3 lt 0 0 5 00 4 lt 3 00 knee Insert Del Save Dom Enter Note Be sure that the temperature decrease is set in a step that lies within a loop Otherwise there will be no iterative temperature decrease 5 5 3 Adjust heating and cooling rates Since the TPersonal is a fast thermocycler it may be necessary to reduce the heating and cooling rate for some applications For example this is helpful if protocols from other Thermocyclers shall be used The heating or cooling rate respectively can be set in the row labelled with A 95 Main funct Te dt s AL 7s 1 3400 2 lt lt 1 08 Er lt 3 00 4 lt 3 00 Info Kasse pr Save Dom Faser Note The default setting 3 00 el indicates maximum ramping 5 6 Run program 5 6 1 Select and start program Open the main screen 32 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company Prog Temp 23 E Lid 24 0 C a ES System rare 019 Edie To run a program press Start Stop BE Start plock 0
43. tions gt Main funct AT dt s CIE li 400 2 3 00 G lt 3 00 As lt 3 00 Paro Insert Del Save Pgm Enter Note To go back to programming sheet move the cursor to the very left side of the display row Main funct Alternatively you can move the cursor to the very right Temp C Time Options gt 1 Josy Oh 5m Os a VJ Oh Im Os ER 54 10 Oh Ilm Os 4 1240 Oh Im OS 2 24 1106 Insert Del Save Pgm Enter Note In the main screen you will find the information that a special parameter has been modified This is indicated by a in the row labelled with opt gt 5 5 1 Program time increments To compensate for loss in enzyme activity each step within a loop can be extended from cycle to cycle Enter the desired time increment seconds in the row labelled with dt s This value will be added to the time value from cycle to cycle Main funct AT C dt s AEN 1 3 00 2 3400 Instruction Manual TPersonal 10 2009 31 Biometra An Analytik Jena Company ER lt Le 3 00 4 C 3 00 Into huser ben Save Dom Heer Note A time increment will have an impact on the total runtime depending on the numbers of cycles and the size of the increment program with many cycles and large time increments will take a significantly longer time than a standard protocol Note A time increment has to be programmed within a loop Otherwise the entered increment will be wi
44. tra An Analytik Jena Company Prog Temp EEG Lid 24 210 TAES System Seide Sie Biete Press Edit to enter the edit mode Se le ET 2 XT 3 I 4 Page NV Name Back Edit program Enter You are now in the directory structure of the edit mode Use cursor keys to select a subdirectory 0 0 ET a 00 2 OM 4 Page NV Name Back Edit program Enter Press Enter to enter highlighted subdirectory The program storages are displayed El en 0 empty 1 empty Edit program 2 empty B 3 empty 4 empty Page 4 Page V Sos Den Edit 5 3 2 Select program store lt gt Use cursor keys to select a program store 23 5 EN Tip Use numeric keypad to directly enter program number 7 8 119 For numbers smaller than 10 enter 0 plus number lol 20 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company El poo S 0 empty 3 1 empty Edit program a 2 empty B 3 empty B 4 empty Page N Page V Coo bel Edit Using the softkeys Page hl and Page V you can scroll through the list in 5 program steps Press Edit or gt to write new program in Program store 03 5 3 3 Enter program name Each program is specified by a program number and a subdirectory number To make retrieval of a program easier you can enter a name for each
45. ure that the modified program will work correctly 5 4 2 Insert program steps Open the programming spreadsheet Temp C Time Options gt 1 95 0 On Sm Os 2 95 0 Oh lm Os Sr 59 Oh Im Os 4 Tae Oh lm Os 2 24 ES Insert Del Save Pgm Enter To insert a program step press Insert Del Insert Delete step La 95 0 Oh 5m Os 23 95 0 Oh lm Os ES 590 Oh Im Os A 12 0 Oh Im Os 2 24 Del Cancel Delete Insert Enter the number of the step that you want to insert delete Example To insert new step at step 3 enter 3 and press Insert Insert Delete step 3 E 95 0 Oh 5m Os 2 95 0 Oh lm Os SR 55 0 Oh Im Os 4 7 0 Oh lm Os 2 24 lt Del Cancel Delete In ze Instruction Manual TPersonal 10 2009 21 Press Insert Biometra An Analytik Jena Company Insert Delete step 8 L 9550 Oh 5m Os 24 95 00 Oh Im Os ER 92 0 Oh Ilm Os 4 71 0 Oh Im Os 2 24 a ring messe ps NS A new step is inserted Temp 10 C Time Oh Om Is Enter temperature and time for new step Temp C E Che H DE Ioa ER L 4 DOs mo oO oO O Time Oh 5m Oh lm Oh Om Oh lm Os Os ls Os Inser pel Seve Pen lime e it Options gt Note Additional steps will be inserted before the selected step The following steps are shifted by one position The new step has been entered within an existing loop this loop now consists of three st
46. wing conditions This warranty is valid for 24 month from date of shipment to the customer from Biometra This warranty will not be extended to a third party without a written agreement of Biometra This warranty covers only the instrument and all original accessories delivered with the instrument This warranty is valid only if the instrument is operated as described in the manual Biometra will repair or replace each part which is returned and found to be defective This warranty does not apply to wear from normal use failure to follow operating instructions negligence or to parts altered or abused 52 Instruction Manual TPersonal 10 2009 15 Subject Index Beep NU 36 CONU Eege 36 ENE 17 Declaration of Conformity 51 Decontamination Certificate 48 Directory SEI Cl EE 19 DISPLAY EP 19 DISPO Alena A PE 50 Firmware update nnannnannnneennnenennennenene 44 le ld mern nen 18 blockade aan en 42 pre heating Renee nennen 22 LANGUAGE ins N isad kui amaan aaa ka 36 Mamtenance munema 44 Operation Voltage ccccccsesseeeeeeeeeeees 13 PACKT 47 Plasticware 1 cc ceecceececeeeceeeeceeeceeeeeeeesaneeaees 45 Program CODY AMT 28 GVO ANC EE 23 SE 30 delete sten 26 Instruction Manual TPersonal 10 2009 Biometra An Analytik Jena Company EHE EEE 26 heating and cooling rates 32 IS En D 27 lid temperature anuma 22 10707 e AM 24 W lu 21 AUIS E
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