Pump22Manual 5.5x8.5_forpdf.qxp
Contents
1. op op Ll WARNING USE IN MANNER NOT SPECIFIED BY THE MANUFACTURER MAY IMPAIR THE PROTECTION PROVIDED BY THE EQUIPMENT 28 22 Harvard Apparatus Syringe Pump Model Appendix F Accessories For All Pumps Foot Switch For rem ote START STOP switch has 9 ft cable terminating in connector to fit 25 pin terminal at rear of pump Press switch to start press to stop Cata og 55 2215 Foot Switch only with cable and connector Reversing Switch This sw rotation itch plugs into the 25 pin connector and changes the direction of motor to produce either infusion or withdrawal It is the same switch that is supplied with infusion withdraw al pumps Catalog 55 2217 Reversing Switch only RS 232 Kit For IBM computers and compatibles Consists of cable with 25 pin connectors at each en d to connect all pump 22 s to the RS 232 port Enables user to write programs to Catal Catal Control all functions remotely Reversing Ramping up and down Control volume delivered og 255 2223 RS 232 Cable for IBM compatibles og 55 2225 RS 232 Cable for non IBM compatibles Daisy Chained Control Cata Cata Cata Cata og 55 2239 Daisy Chain Connector og 4554145 Computer to Daisy Chain for IBM Computers og 2554146 Computer to Daisy Chain for non IBM Computers og 155 7760 Daisy Chain to Daisy Chain2. connector on rear panel RS 232C Lines Pin 1 GND Pin 2 RXD Receive data from computer input Pin 3 TXD Transmit data to computer output Pin 7 GND Pin 19 TXD Transmit data to daisy chain output Pin 21 RXD Receive data from daisy chain input Make no connection to other pins for RS 232C interface A normally open momentary switch may be connected between Pin 18 and Pin 7 A momentary closure vvill start the pump if it is stopped and stop it if it is running This interface should not be used in conjunction with RS 232C 26 22 Harvard Apparatus Syringe Pump Model Appendix D Contd 2 Baud rate Baud rate may be set to 300 1200 2400 or 9600 baud The setting is preserved in nonvolatile memory To set baud rate Press SET and START STOP key simultaneously The display will show the current baud rate Each time START STOP is pressed the display will change showing 300 1200 24 2400 or 96 9600 Pressing ENTER will select the currently displayed baud rate The display will return to its normal condition displaying flow rate 3 Front panel indicator REMOTE lamp indicates that pump is under remote control When lit the key board is disabled Indicator lamps and the numeric display continue to function when under remote control 4 Communication RS232C format is No Parity 1 Start 8 Data bits 2 Stop bits Transmissions to the pump consist of sequences of ASCII characters terminated w
3. UT Enter Command K T 1 KS LOC 1 gt THEN 85 SS INPUTS LOC 1 1 RIGHTS S 1 OR PS gt OR P OR 110 O 70 T The Pump Response is S O 40 Line 40 accepts a string which is sent to the pump in line 50 Line 50 also sends an end of line character Carriage Return When the end of line character is received by the pump it interprets the preceding characters and sends its response The response will depend on the particular command received but it will always end with one of the four prompt characters which indicate its status Pum Pum Pum Pum p Stopped p Infusing p Reversing p Stalled Line 80 sets P to the last character received The program continues concatenating characters to S until a prompt is received 8 Example 3 Interpreting the Response Example 3 demonstrates a method of breaking up the pump response transmission into its constituent parts 10 20 30 40 50 60 70 80 90 100 110 REM CLS OPE INP PRI GOS PRI PRI IF IF IF EXAMPLE3 COM1 1200 N 8 2 RS CS DS AS 1 UT Enter Command COMMAND T 1 COMMANDS UB 150 T Response RESPONSE T status n PS THEN PRINT STOPPED PS gt THEN PRINT RUNNING PS lt THEN PRINT REVERSING RS 232 Tutorial Contd 16 120 IF P THEN PRINT STALLED N 130 PRINT s 140 GOTO 40 150 RE KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK 1
4. Cable Computer to Pump Connection The Model 22 Infusion Pump uses an RS232C interface to communicate with a computer This is the same communication system used by most terminals and modems and many other laboratory instruments On the IBM PC and compatible computers this interface is usually called a serial or COM port The computer should have one or two 25 position pin male connectors on the rear panel Note Any 25 position socket female connectors on the computer are parallel printer interfaces that are not suitable for controlling the pump Care should be taken to ensure that the male end of the pump cable is not connected to the computer The Model 22 pump has a 25 position socket female connector The cable wiring for use with a standard IBM PC or compatible serial port is shown in Figure 1 To connect the pump to the computer Turn off both devices Connect the socket end of the cable to the computer Connect the plug end to Pump 22 Tighten the retaining screws Boot up the computer and turn on Pump 22 2 22 Harvard Apparatus Syringe Pump Model RS 232 Tutorial Contd Once the cable is connected both the computer and the pump may be used normally Either one may be turned on or off at any time It is good practice hovv ever to turn off power when connecting or disconnecting the cable 3 Setting the Baud Rate RS232C is a bidirectional serial data communications interface This mean
5. Harvard Apparatus Syringe Pump Model General information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied war ranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted f
6. be accepted by the pump Leading zeros and trail ing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number received will be rounded to four significant digits if the leading digit is 1 or three significant digits if the leading digit is 2 to 9 Queries Response CRLF value CR LF prompt Queries with Numeric Response DIA Send diameter value units in mm RAT Send rate value in current range units VOL Send current accumulated infused volume units are ml TAR Send target volume units are ml VER Send model and version number currently 22 900 Value format nnnn nnn The transmitted value is an 8 character string with leading zeros converted to SP char acters ASCII 32 The fifth character is a decimal point ASCII 46 24 22 Harvard Apparatus Syringe Pump Model Appendix C Contd Query Response CRLF range CR LF prompt Queries with String Response RNG Send range message Range is a character string one of ML H ML M UL H or UL M Error responses CR LF CR LF prompt Unrecognized command CRLF O O R CR LF prompt Out of range Appendix D 2 22 Interface Specification for Model 22 Infusion Pump RS 232C Cable Wiring SHIELD 25 Pin D Sub Socket 8 4 9 9 on COMPUTER 25 on m om on m 19 18 7 46 15 14 pri m R n 8 x m s Harvard Apparatus Syringe Pump Model 1 Connector Female 25 pin D
7. do not behave as described make sure that the cable is properly connected to COM1 and that the pump baud rate is set to 1200 Make sure your cable is wired according to Figure 1 If you are using COM2 or are not sure which port you are connected to change line 20 to 20 OPEN COM2 1200 N 8 2 RS CS DS AS 1 and try again Assuming that the computer and pump are responding correctly type RUN enter The pump will begin to infuse and the character gt will be displayed Try some other commands from the table in Appendix The computer monitor will appear similar to Figure 2 Since this program loops continuously press the CTRL and BREAK keys together to stop it Keyboard Command Script GW BASIC 2 02 C Copyright Microsoft 1983 1984 60892 Bytes free OK load example 1 OK run run gt gt rat 999 000 gt rng UL M gt ulm 123 4 gt rat 123 400 gt stp 4 22 Harvard Apparatus Syringe Pump Model RS 232 Tutorial Contd 7 Example 2 Handling the Pump Response The character by character technique used in Example 1 is suitable for keyboard entry of commands however program control of the pump requires that a program wait until a complete response has been received Example 2 illustrates how to do this 10 20 30 40 50 60 70 80 90 100 110 120 REM OPE EY INP PRI S IF PS IF GOT PRI GOT EXAMPLE2 COM1 1200 N 8 2 RS CS DS AS 1 OFF
8. la 60 29 10 250 2 30 Becton Dickinson 500 3 26 Plastic Plasti pak Sherwood M onoject 1 0ml 4 61 mm Size Diameter 2 5 7 28 1 478mm Plastic 5 10 30 3 8 66 Size Diameter 10 14 57 9 12 06 1 14 65 mm 10 14 50 3 04200 nxoxsassa nsunuak 2 7 i is Hamilton M icroliter 50 60 26 70 20 2040 Series Gastight 35 23 80 Size Diameter 60 26 60 5yl 0 103 mm Ud n 140 38 40 1 0 1457 Air Tite 2 0 206 All Plasti 2 7 Size Diameter Popper amp Sons Inc 0 729 2 5 cc 9 60mm Perfektum Glas bo 1 031 5 0 12 45 Size Diameter 100 1 46 10 15 90 0 25 cc 3 45mm 250 2 3 20 20 05 0 5 3 45 500 3 26 20 1 4 50 1 0ml 4 61 mm 50 29 00 2 8 92 25 7 28 3 8 99 5 10 3 nis ae aires 5 11 70 10 14 57 Unimetrics 7 25 23 0 Series 4000 amp 5000 30 22 70 50 32 6 Size Diameter 50 29 00 10 11 0 460mm 100 35 70 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 Appendix B 22 Nominal Minimum amp Maximum Flow Rates 22 Various Syringes Actual Limits VVill Vary Depending on Manufacturer Pump 22 SYRINGE HL HOUR UL MINUTE ML HOUR ML MINUTE SIZE 0 5 ul 002 23 8 1 0 ul 003 47 8 2 0 ul 006 95 2 5 0 ul 015 238 0 10 0 ul 029 474 0 25 0 ul 073 1193 0 50 0 ul 002 39 7 100 0 ul 005 79 7 250 0 ul 012 197 8 500 0 ul 1024 397 0 1000 0 ul 048 795 0 1 0m 049 805 0 2 0m 011 186 6 25m 010 168 2 3 0 m 011 181
9. 3 310 IF ASC MID S I 1 13 THEN 330 320 I I41 GOTO 310 330 RESPONSES MIDS S 3 I 3 340 RETURN The subroutine at lines 150 340 is called after each transmission to the pump This ensures that the pump has executed the command and finished transmitting its response before the program continues Each time a command is received by the pump the REMOTE lamp on the key pad illuminates and the pump key pad is locked out Line 50 illustrates use of the KEY command which allows the programmer to re enable the key pad Line 62 illustrates the best way to send commands with numeric arguments The PRINT 1 USING statement ensures that the argument is transmitted in a format which is acceptable to the pump The test at line 64 is necessary to detect out of range responses to the prior command If the target volume is out of range then RESPONSES will equal DOR The loop at lines 70 74 reads the pump s volume accumulator and tests the prompt to determine when the dispense is complete Line 80 detects the stall condition if the pump has reached the end of its travel RS 232 Tutorial Contd 18 10 Example 5 Ramping Flow Rate A useful feature of Pump 22 is its ability to change rate without stopping Example 5 illustrates variable flow rate infusion 10 REM EXAMPLES 14 KEY OFF CLS 18 OPEN COM1 1200 N 8 2 RS CS DS AS 1 22 PRINT 1 STP GOSUB 150 26 PRINT 1 CLT GOSUB 150 34 INPUT Enter Range MLM M
10. 4 5 0m 019 317 0 10 0 m 1028 461 0 20 0 m 050 821 0 30 0 m 074 1208 0 50 0 m 002 28 40 Harvard Apparatus Syringe Pump Model 100 0 m 003 47 60 140 0 m 004 55 10 23 22 Harvard Apparatus Syringe Pump Model Appendix C Pump 22 Interface Command Reference After each transmission to the Pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The prompt character indicates the status of the pump as follows ASCII 58 decimal ASCII 62 decimal lt When running reverse ASCII 60 decimal When stalled ASCII 42 decimal Note With a pump chain if no address precedes the command transmitted the pump at address 0 will respond to the command When stopped gt When running forward Commands Response CR LF prompt KEY Return to keyboard control Remote lamp off RUN Infuse forward direction REV Start reverse direction STP Stop CLV Clear volume accumulator to zero CLT Clear target volume to zero dispense disabled MLM number _ Set rate units are milliliters per minute ULM number Set rate units are microliters per minute MLH number Set rate units are milliliters per hour ULH number Set rate units are microliters per hour MMD number Set diameter units are mm Rate is set to MLT number Set target infusion volume units are ml Numbers between 0 and 1999 will
11. 60 RE get response subroutine 170 RE KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK 180 REM returns 190 REM PS prompt character 77 4 or 200 EM RESPONSES response string if syntax error 210 RE OOR if out of range 220 RE if prompt only 230 RE 240 S RESPONSES 250 IF LOC 1 gt THEN SS SS INPUTS LOC 1 41 260 PS RIGHTS S 1 270 IF PS gt OR P OR P OR P THEN 290 280 GOTO 250 290 IF LEN S 5 THEN RETURN 300 1 3 310 IF ASC MID S I 1 13 THEN 330 320 I I41 GOTO 310 330 RESPONSES MID S 3 I 3 340 RETURN Harvard Apparatus Syringe Pump Model The subroutine at lines 150 340 is designed to be called after each transmission to the pump The pump terminates each line of its response with the two character sequence Carriage Return Line Feed SeeAppendix 1 for details Lines 310 320 search for the Carriage Return character ASCII 13 and line 330 extracts the response string 9 Example 4 Volume Dispensing Example 4 uses the same subroutine as the last example and demonstrates the volume dispensing capabilities of the pump Several useful programming techniques are also illustrated 10 REM EXAMPLE4 20 CLS KEY OFF 30 OPEN COM1 1200 N 8 2 RS CS DS AS 1 32 PRINT 1 STP GOSUB 150 36 PRINT 1 RAT GOSUB 150 RATES RESPONSES 38 PRINT 1 RNG GOSUB 150 RANGES RESPONSES 40 PRINT Current r
12. Cable Harvard Apparatus pumps are distributed on a non exclusive basis by INSTECH Instech Laboratories Inc Headquarters 5209 Militia Hill Road Plymouth Meeting PA 19462 TL 800 443 4227 TL 610 941 0132 FX 610 941 0134 European sales office P O Box 2 9950 AA Winsum The Netherlands TL 0031 0 594 696123 www instechlabs com Pumps syringe
13. KK kk kk kk 150 REM get response subroutine version 2 152 REM Y x x x k x x x x x x kk KKK KK KK kk KK KK KK KK KK KK kk kk kk 160 S P e RESPONSES 19 22 Harvard Apparatus Syringe Pump Model RS 232 Tutorial Contd 170 WHILE P AND P AND P AND P 180 IF LOC 1 gt THEN S SS INPUTS LOC 1 41 190 PS RIGHTS S 1 200 WEND 210 IF 55 24 THEN RESPONSE MID S 3 INSTR 3 S CHR 13 3 220 RETURN Lines 54 and 74 test the extreme rates if they are vvithin range then the intermediate rates will be acceptable R is the range command string Note the use of the PRINT 1 USING statement in lines 52 and 70 to allow rate setting in any one of the four ranges Line 84 sets up a timer interrupt which causes the subr outine at line 100 to be executed one per second The interrupt is turned on and off with the TIMER statements The variables P andT are tested by the main program and modified by the interrupt subroutine Line 120 computes RATE as a function of the current time T the duration TFINAL and the initial and final rates Other flow rate functions can be programmed by changing this line The subroutine at line 150 is amore elegant version of the get response routine using aWHILE WEND loop and the INSTR function Its behavior is identical to the earlier version 11 Other Languages Any language which supports RS 232C communication may be us
14. LH ULM OR ULH R 38 IF R MLM OR R MLH OR R ULM OR R ULH THEN 50 42 IF R mlm OR R mlh OR R ulm OR R ulh THEN 50 46 PRINT Invalid Range GOTO 34 50 INPUT Enter Final Rate FRATE 52 PRINT 1 USING RS H ERATE 54 GOSUB 150 IF RESPONSES THEN 66 62 PRINT Out of Range GOTO 50 66 INPUT Enter Initial Rate IRATE 70 PRINT 1 USING RS H H H IRATE 74 GOSUB 150 IF RESPONSES THEN 80 78 PRINT Out of Range GOTO 66 80 PRINT 41 RNG GOSUB 150 RANGES RESPONSES 82 INPUT Enter Ramp Duration Seconds TFINAL 83 PRINT PRINT Press STOP START on pump to interrupt 84 ON TIMER 1 GOSUB 100 85 PRINT 41 run GOSUB 150 86 TIMER ON 90 IF P z THEN 94 92 TIMER OFF PRINT Interrupted PRINT GOTO 22 94 IF T TFINAL THEN 90 95 TIMER OFF 96 PRINT Done PRINT GOTO 22 Harvard Apparatus Syringe Pump Model 22 100 REM kkxkxkxkkkxkxkkkkkkkkkkkkkkkkkkkkkkkkkxkkkkkkkxkx k 104 REM timer subroutine executed once a second 106 REM kkxkxkxkkxkxkxkkkxkkkkkkkkkkkkkxkkkkxkkkkkxkkkkkkkxkx k 110 T T 1 120 RATE IRATE T FRATE IRATE TFINAL 122 PRINT USING t RANGES T RATE 130 PRINT 1 USING RS HHHE RATE 140 GOSUB 150 141 PRINT 1 KEY GOSUB 150 142 RETURN 148 REM Y x x x x x x x x kk kk x x kk kk kk Ck Ck Ck Ck Kk ok Ok ok KK
15. Model 22 Syringe Pump Series User s Manual Model 22 Syringe Pump 05 2222 Model 22 Infuse Withdraw 55 2226 Model 22 Miltisyringe 59 5920 Table of Contents r SUBJECT PAGE NO Table 1 1 9 General Information Warranty and Repairs 2 b Specifications iniri dna aaa ua iu arida 3 WEE T Uc er 4 Initial StU iis 5 5 Loading Syringe 6 n Pump Operallonhisuso vocis oe tick onini Aaz 7 v Entering Syringe Diameter S 7 a Entering Flow Rate s 7 b Out of 8 5 Changing Flow Rates 8 E End of TraVelasadgi 8 Loss Of POW lice oxic d r 8 Maiienancee assa ette 8 a a 9 WME 10 Non TTL 10 RS5 232 Tutorial aiii 11 Computer Control of Multiple Pumps 19 Appendices A Table of Syringe Diameters 21 B Nominal Minimum and Maximum Flow Rates 22 C Interface Command Reference 23 D Interface Specifications 25 E Malnitetialiogius uon bkn cocoa de al 27 I JACODSSOLIGS conico 28 4 22
16. aracters have been received from the interface If the value of the function is less than one then no characters have been received and the program loops back to line 40 continuously testing the keyboard If characters have been received then they are transferred to the string variable S using the INPUT function in line 80 Line 90 displays the received characters and the program loops back to line 40 6 Keyboard Commands Via RS 232C To test the program and interface Turn off the pump Turn it on again the pump display should be blinking Runthe program 14177 22 Harvard Apparatus Syringe Pump Model RS 232 Tutorial Contd At this point nothing will appear on the monitor since the program is looping continuously waiting until either a key is pressed or a character is received from the pump To make something happen press the enter RETURN key of the computer keyboard The following should occur The REMOTE lamp of the pump illuminates The pump display stops blinking The character appears on the computer monitor What has occurred is that the character corresponding to the enter key Carriage Return has been read by the program and sent to the pump The pump has responded with a prompt character which indicates that it is stopped This has been received by the program and output to the computer display The program is still looping waiting for the next event to occur If the pump and computer
17. ate is RATES RANGE 42 INPUT Change Rate Y or N K 44 IF Y OR KS y THEN 50 46 IF N OR K n THEN 60 48 GOTO 42 50 PRINT 41 KEY GOSUB 150 52 PRINT Change rate from pump keypad RS 232 Tutorial Contd B 54 PRINT Press any computer key to continue N 56 IF INKEYS THEN 56 58 GOTO 32 60 INPUT Enter volume to dispense ml VOLUME 62 PRINT 1 USING MLT VOLUME GOSUB 150 64 IF RESPONSES THEN 66 65 PRINT Error Try again GOTO 60 66 PRINT 1 CLV GOSUB 150 68 PRINT 1 RUN GOSUB 150 70 PRINT 1 VOL GOSUB 150 72 PRINT Volume dispensed RESPONSES 74 IF P gt THEN 70 80 IF P x THEN 90 82 PRINT Done PRINT GOTO 32 90 PRINT Stalled PRINT GOTO 32 150 RE KKEKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK 160 RE get response subroutine 170 RE KKEKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK 180 REM returns Harvard Apparatus Syringe Pump Model 190 REM P prompt character Ws 3 Wem 200 REM RESPONSES response string if syntax error 210 RE OOR if out of range 220 RE if prompt only 230 RE 240 S RESPONSES 250 IF LOC 1 gt THEN S SS INPUTS LOC 1 1 260 P RIGHT S 1 270 IF P OR P 2 OR P OR 5 x THEN 290 280 GO TO 250 290 IF 55 lt 5 THEN RETURN 300 1
18. ctor at the back of the pump and reverses the direction of the motor These pumps are also provided with adjustable hardware on both the pusher and the syringe holder Adjust these retainers using the black thumb screws so that both the head of the plunger and the flange on the barrel are held securely in place with minimal free play 22 Pumps with Multiple Syringe Holders Catalog Nos 55 5920 and 55 2219 These Versions of the Pump 22 have a saw tooth syringe holder capable of holding up to ten syringes emptying them simultaneously at identical rates This multiple syringe will accommodate up to Ten syringes of any size up to 10 ml Six syringes of any size up to 50 ml Catalog Nos 55 2314 and 55 2316 have a multiple syringe holder with accommodates up to four syringes of 30 ml up to 140 ml To load syringes rotate the spring loaded sponge rubber retainer arm out of the way and lay syringes in V shaped slots with the syringe flange touching the holder Rotate the retainer arm to hold syringes in place If less than the maximum number of syringes are used try to place them symmetrically about the lead screw to present a balanced load Note The back pressure for each syringe will be divided by the number of syringes used Thus for four 50 oc syringes the maximum pressure developed in each syringe will be 400 mm Hg 8 psi 7 22 Harvard Apparatus Syringe Pump Model Operating the Pump The syringe diamet
19. ed to control the pump Computer Control of Multiple Pumps Daisy Chain Option A maximum of ten Pump 22 s can be remotely controlled from a single computer RS 232C port by assigning to each pump a unique address from 0 to 9 which will serve as the pump s identifier In addition Pump 22 s can be inter mixed on the same daisy chain with other daisy chainable pumps from Harvard Apparatus Note Each pump in the chain must have a unique address Keyboard Operation To assign an address identifier to a pump press SET and 0 22 Harvard Apparatus Syringe Pump Model Computer Control of Multiple Pumps Contd The display will show Ad n where n is a number from to 9 indicating the pump s current identifying address Press the number from 0 to 9 representing the pump s address The number in the display represented by n above will change to the number pressed Press ENTER when the desired address is displayed Remote Operation When transmitting a command to the pump precede each command with the address of the pump For example the following command will request a pump with address 1 to run 1 RUN CR Only the pump whose address matches the address preceding the command will respond to the command all other pumps will ignore the command Only pumps which have recognized its address on a command will enter remote mode indicated by the REMOTE lamp illuminating When a pump responds to a c
20. er and flovv rate must be entered into the pump s memory using the key pad and LED display See figure belovv ul min mi min NM run MB ul hr ml hr mm diam remote Hill 5 6 7 8 9 rate diam 0 1 2 3 4 enter set Front panel indicating that pump is running at 22 2 ml minute under remote control from a computer The display reads 22 2 while the ml min unit light remote and the run light are illuminated 1 Entering the Syringe Diameter a Identify the make and capacity of the syringe to be used using the diameter table on page 3 or attached to the line cord Referring to this syringe diame ter table determine the diameter in mm If your syringe is not listed deter mine the diameter of the plunger using a micrometer and note it for future reference Depress the SET and DIAM keys simultaneously This procedure prevents accidental changes The mm diam light will flash indicating that the pump is ready to receive input Using the key pad enter the syringe diameter complete with decimal point watching the result on the display If you make an error continue entering numbers until the display goes blank and then enter the correct value When the display reads the desired diameter press ENTER The mm diameter indicator light will go out The syringe diameter has now been entered into memory Each time a diameter
21. inations of syringe size and flow rate are outside the range of the pump such as asking for ul hour with a 100 ml syringe The pump will reject this input data when the ENTER key is pressed and the display will read oor until a dif ferent combination of syringe and rate that is within range is entered See Table 2 for typical maximum and minimum rates for various size syringes Changing Flow Rate To change the flow rate while the pump is running press SET and RATE simultane ously The display will go blank Enter the new flow rate The pump will continue to run at the old flow rate until the ENTER key is pressed at which time the pump will run at the new rate End of Travel The pump motor is equipped with an optical rotation sensor If for any reason an electrical step signal to the motor is not followed by a mechanical step of the motor the power to the motor is shut down and the RUN light will flash indicating the pump is stalled This can occur when the syringe plunger hits bottom a line is kinked syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and press START The pump has an adjustable collar on the rear guide rod This collar can be pre set to terminate pumping at any point It is also useful when small microliter syringes are being used since the pusher block can bend the tiny wire plungers Set the c
22. is programmed the rate is set to zero To recall the syringe diameter entered at any time even while the pump is running simply press DIAM and the diameter will appear on the display without affecting flow while the key is depressed 2 Entering Flow Rate a Determine the range of flow rates ml min ml hour ul min or ul hour for your use Press SET and RATE keys simultaneously One of the four range lamps will flash Each successive press of the RATE key will advance the flow rate range Stop when the desired range is flashing Enter the numerical value of the flow rate desired including decimal point if you make an error enter numbers to clear the display When the correct flow rate value is displayed press ENTER The pump is now programmed for flow rate The display will indicate the numerical value and the units of flow rate will be illuminated 4 22 Harvard Apparatus Syringe Pump Model Operating the Pump Contd Start pump by pressing STOP START key and the pump vill run and the RUN light will be illuminated Stop pump by pressing STOP START key and the RUN light will go out If SET and START STOP are pressed simultaneously the baud rate is displayed and the remote lamp will flash Press ENTER to return to normal operation The remote lamp when illuminated indicates that the pump is under remote computer control These features are covered in detail in the RS 232 instructions Out of Range Certain comb
23. ith CR carriage return ASCII 13 decimal Upper case ASCII 64 to 95 decimal and lower case ASCII 96 to 127 decimal are equivalent ASCII characters from to 12 and 14 to 31 are ignored SP space ASCII 32 decimal is ignored Whenever a transmission is received the pump enters remote mode illuminates the REMOTE lamp and disables the keyboard 27 22 Harvard Apparatus Syringe Pump Model Appendix E Maintenance Pump 22 requires no special maintenance other than keeping it clean by avoiding acci dental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated periodically with the Magnalube G R grease provided with the pump ThisTeflon R based grease is available from Harvard Apparatus Catalog 0606 086 Teflon R based grease Solvents of any type should never be used to clean the pump A mild detergent solu tion may be used to clean the keypad Fuse Replacement Make sure power cord is disconnected from main supply before replacing fuse Remove bottom cover on pump by removing four 4 rubber feet and two 2 screws Locate fuse on power supply module Remove fuse from fuse clip Use caution not to break the fuse when removing Replace fuse bottom cover screws and rubber feet For continued fire protection replace fuse only with 250V fuse of the specified type and rating 3AG 1 2 AMP 250V SLO BLO i FUSE 1 2 AMP S B
24. nd calibrates the flow rate for that syringe A number of special features result from use of microprocessor technology Out of Range If the user selects a combination of syringe size and flow rate that is outside the range of the pump eg 50 ml min with a 10 microliter syringe the pump will reject this instruction and notify the user Nonvolatile Memory The pump remembers its last instructions entered from the keyboard including diameter and flow rate even after the power has been disconnected Non Flow Detection An optical sensor detects lack of motion due to jamming end of syringe travel kink ing of tubing etc shuts down the pump and alerts the user Momentary Loss of Power The pump will stop and alert the user RS 232 Interface The pump is provided with a built in interface for use with most laboratory comput ers A Users Manual for RS 232 application is enclosed TTL Logic The pump can be stopped started and reversed remotely m 22 Harvard Apparatus Syringe Pump Model Initial Set Up Read the Manual Locate the voltage selector switch on the rear panel of the pump see below and set it to the voltage being used If other than 115V 60 Hz is being used the plug must be cut off and an appropriate plug installed observing the polarity of the international line cord used Brown high Blue neutral Green ground Turn on main power switch located directly above the line cord on the rear
25. ollar to stop the pump before the microliter plunger hits bottom Momentary Loss of Power If during the course of pumping theA C power has been interrupted the pump will stop running and will not resume when the power goes on This condition results in the display flashing and the RUN light going out To resume pumping press the START key Maintenance Pump 22 requires no special maintenance other than keeping it clean by avoiding accidental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated periodically with the Magnalube G R grease provided with the pump ThisTeflon R based grease is available either from Harvard Apparatus or Carleton Stuart Corp 13 02 44th Ave Long Island City New York 11101 Solvents of any type should never be used to clean the pump A mild detergent solu tion may be used to clean the key pad s 22 Harvard Apparatus Syringe Pump Model Remote Control All new Model 22 Pumps are equipped with both TTL Logic and a built in RS 232 Interface via the 25 pin connector at the rear TTL Logic Transistor to Transistor Logic TheTTL Logic exists in all pumps manufactured after April 1 1988 with serial numbers A 24090 or higher TTL is a system of control in which there are only two states HIGH In which a voltage of 2 5 5 0 volts is applied to a pin LOW In which aTTL voltage of 0 0 5 volts is applied In this application low is a short circuit to gr
26. ommand preceded by its address it terminates its response with its prompt character preceded by the pump s address For example with the above command the pump will respond with CR LF 1 To just request a pump s current prompt send just the pumps address 1CR The addressed pump will respond with its prompt preceded by its address By default if the pump address is omitted from any command address 0 will be used and the pump with address 0 will respond to the command but will not return its address before the prompt Pump Daisy Chain to Computer Connection For each Pump 22 in the Daisy Chain Attach a 25 Pin to Daisy Chain Adapter 55 2239 to the RS232 Connector on the rear of each pump in the Daisy Chain Attach a 25 Pin to Daisy Chain Cable 55 4145 for IBM and compatibles or 55 4145 for other computers from the computer s RS 232 port to the socket labeled IN on the Daisy Chain Adaptor of the first pump For each additional pump in the Daisy Chain after the first pump attach a Daisy Chain to Daisy Chain Cable 55 7760 from the socket labeled OUT on the previous pump to the socket labeled IN on the next pump 22 Harvard Apparatus Syringe Pump Model Appendix A Syringe Diameters in mm Size Diameter Size Diameter Scientific Glass 8cc 9 525 mm 3cc 8 95 mm Engineering PU cU en 5 13 00 Size Diameter 50cc 28 600 10 15 80 25 ul 0 73 mm 100cc 34 900 20 20 15 50 1 03 30 23 10 100 1 46 we al
27. or return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and is not for clinical use on human patients CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS 7 22 Harvard Apparatus Syringe Pump Model Specifications Specifications Type Size LxWxH Weight Power Leakage to Ground Ground Resistance Voltage Operating Range Drive Motors Timing Belt Drive Lead Screw Pitch Motor Steps per 1 Revolution of Lead Screw Step Rate inimum aximum Pusher Advance S tep Pusher Travel Rate inimum aximum Dynamic Speed Range Accuracy Reproducibility Calibration Syringe Size for Pump 22 with Holders for Up to 2 Syringes Up to 4 Syringe
28. ound 14 15 16 17 18 19 20 21 22 23 2 2 AA Vcc 5 V lt q Running Indicator TXD RXD Daisy Chain Reverse Foot Switch Note This view is from within the pump looking outwards throuth this Connector All pin numbers refer to the 25 pin D Sub connector 10 22 Harvard Apparatus Syringe Pump Model TTL Functions Pump Condition Outputs Available Pin 15 High output 2 5 5 0 volts when pump running Low output 0 0 5 volts when pump is stopped or stalled Forward Reverse Pin 16 High Normal Running in Infusion Low Short to ground pump reverses and withdraws Remote START STOP Timer Pin 17 High Normal Running Low Short to Ground Pump Stops Remote START STOP Momentarily Footswitch Pin 18 When momentary shorted to ground if pump is stopped it starts if pump is running it stops Note that pump will not respond to a command of less than 50 millisecond duration Power amp Ground Pin 1 Ground Pin 7 Ground Pin 14 5 Volts in series with 47 ohm resistor Current drawn should not exceed 25 milliamps Non TTL Pin Functions Pins 1 Used for RS 232 computer control Pins 2 B Used for RS 232 computer control Pins 3 Used for RS 232 computer control Pins 7 a Used for RS 232 computer control Pins 19 Used for RS 232 computer control Pins 21 Used for RS 232 computer control 22 Harvard Appa
29. panel The display will now illuminate indicating that the power connections are correct The flashing number displayed is the current flow rate Flashing indicates that power has just been applied Rear panel showing OFF ON switch and voltage selector set for 115V m 22 Harvard Apparatus Syringe Pump Model Loading Syringes Model 55 2222 will hold one or two syringes from 140 ml capacity to 0 5 ul To load syringes release the black pusher block by depressing the bronze button and sliding the block to the left Lay the syringe s on the black syringe holder where they are held in place by the spring loaded fingers Make sure that the flange on the barrel of the syringe touches the syringe holder Advance the pusher block until it touches the head of the syringe At this point the pusher can be advanced manually to clear any air bubbles from the syringe and lines To use small diameter syringes it will be necessary to unscrew the black knob that retains the syringe holder fingers remove the fingers invert them and replace Model 55 2275 has anti siphon protection Both the pusher block and syringe holder have adjustable retainers to secure both the plunger and flange of the barrel 22 Pumps with Infusion and Withdrawal Models 55 2226 55 2219 and 55 2316 are infusion and withdrawal versions of the 22 Pump A reversing switch is provided This switch plugs into the 25 pin RS 232 conne
30. preter may be called GWBASIC or BASICA This tutorial assumes a rudimentary knowledge of programming in BASIC Refer to the manuals supplied with the operating system to learn how to start the BASIC interpreter edit BASIC programs run and debug programs and save and load programs on diskettes The RS232C interface operates one bit at a time However the interface is character oriented from the programmer s point of view Programs send characters to the interface and receive them from the interface in the same way they accept characters from the keyboard and display them on the monitor Each operation is controlled independently by the program which is running Example 1 demonstrates how to tie the keyboard display and interface together so that the data transmission can be controlled and observed RS 232 Tutorial Contd B 5 Example 1 Terminal Emulator Start the BASIC interpreter At the OK prompt type in EXAMPLE1 22 10 REM EXAMPLE1 20 OPEN COM1 1200 N 8 2 RS CS DS AS 1 30 KEY OFF LOCATE 1 0 7 40 K INKEY 50 PRINT KS 60 PRINT 1 KS 70 IF LOC 1 lt 1 THEN 40 80 55 INPUTS LOC 1 1 90 PRINT S 100 GOTO 40 interface Harvard Apparatus Syringe Pump Model This sort of program is usually called a terminal emulator since it connects the key board display and RS 232C interface so that they function similarly to acomputer terminal Its use here is to permit testing and experimenta
31. ratus Syringe Pump Model RS 232 Tutorial Tutorial Contents Tutorial Contents Contd 1 Introduction 9 Example 4 Volume Dispensing 2 Computer to Pump Connection 10 Example 5 Ramping Flovv Rate 3 Setting Baud Rate 11 Other Languages 4 Starting BASIC 5 Example 1 Terminal Emulator Appendix 1 Pump 22 Interface 6 Keyboard Commands via RS232C Command Reference 2 7 2 Handling the Pump Appendix Il Interface Specification sponse for Model 22 Infusion 8 Example 3 Interpreting the P ump Response Tutorial Step by Step 1 Introduction The purpose of this manual is to introduce users of Pump 22 to the use of the built in RS 232C computer interface It is designed to assist those who are not familiar with computer control of laboratory instruments to write programs in BASIC on IBM PC or compatible computers Experienced programmers may prefer to skip to the technical specification in Appendix II The example programs are intended to show the fundamentals of communication to the instrument and to serve as building blocks when writing programs tailored for a specific application Each example program builds on the previous one They include programs that permit keyboard control of the pump and demonstrations of automatic volume dispensing and variable flow rate operation A pre wired cable for interface to IBM PC or compatible computers is available from Harvard Apparatus Order catalog number 55 2223 IBM to Model 22 RS232C
32. s Up to 6 Syringes Up to 10 Syringes Microdialysis Up to 4 Syringes Flow Rate Range Minimum Maximum Display RS 232C Interface Non Volatile Memory Microprocessor multiple syringe infusion or infusion withdrawal 11 x 8 75 x 5 5 inches 280 x 220 x 140 mm 10 Ibs 4 5 kg 115 230 VAC 50 60Hz via selector switch 45 Watts Typically less than 15 micro amps Typically less than 0 2 ohms 100 125 VAC 200 250 VAC 0 9 microprocessor controlled stepping motor 2 1 reduction from motor 24 threads per inch 3200 1 4 stepping 6 8 seconds per step 416 7 microseconds per step 0 330729 microns minimum 2 9068 u m min 41 6 mm min 16 384 to 1 1 0 1 Automatic enter syringe size up to 50 mm Glass plastic or stainless steel From 0 5 ul to 140 ml From 30 ml to 140 ml From 0 5 ul to 50 ml From 30 ml to 10 ml From 0 5 ulto 10 ml 0 002 ul hr with 0 5 ul syringe 55 1 ml min with 140 ml syringe 3 1 2 digit numeric LED 7 LED indicators for range diameter Run Stop and remote control Chained dual bidirectional ports Storage of all settings m 22 Harvard Apparatus Syringe Pump Model Features Pump 22 is an Infusion Only syringe pump employing a microprocessor controlled small step angle stepping motor driving a lead screw and half nut A key pad is used for data entry of flow rate and syringe diameter The microprocessor calculates the cross sectional area of the syringe to be used a
33. s that in each direction computer to pump and pump to computer data is transmitted one bit at a time The rate of data transmission in bits per second is the baud rate The Pump 22 will operate at baud rates of 300 1200 2400 or 9600 To Set The Baud Rate Press SET and START STOP keys simultaneously The display will show the current baud rate and the REMOTE lamp will blink Each time the START STOP key is pressed the display will change showing 300 1200 24 2400 or 96 9600 Pressing ENTER will select the currently displayed baud rate The display will return to its normal condition displaying flow rate The REMOTE lamp will extinguish The Pump 22 may be used with an IBM PC or compatible computer at any of these baud rates The fastest rate 9600 baud is recommended The examples assume that the pump has been set for 1200 baud The pump baud rate is preserved in non volatile memory it does not need to be reset each time the pump is turned on Starting Basic The serial interface in the IBM PC or compatible computer contains the hardware necessary to communicate with the pump To control this hardware a program is required All of the example programs are written in the BASIC language which is supplied with the operating system of the computer Depending on which kind of computer is used the operating system may be PC DOS Compaq DOS or MSDOS Whichever it is Version 2 0 or higher should be used The BASIC inter
34. tion with the Pump 22 The OPEN statement in line 20 initializes the interface and assigns it a file number An OPEN statement must be executed once before attempting to use an interface The file number 1 in this case may be any value from 1 to 255 and will be used by subsequent statements to refer to this interface There may be other OPEN statements in a program for other interfaces or disk files if so they should use a different file number The particular RS 232C port is identified by COM If your computer has two ports then COM2 may also be used The statement sets the COMI baud rate to 1200 and defines the particular format of serial bits to be used With Pump 22 this Is no parity bit eight data bits per character and two stop bits Line 30 clears the bottom line of the screen and turns on the flashing cursor Line 40 reads the keyboard and assigns a single character string to string variable K if a key has been pressed This statement does not wait for a keystroke if no key has been pressed then the null string is assigned to K Line 50 sends the character to the display and line 60 sends it to file 1 which has been assigned to COM1 These statements connect the computer keyboard to both the display and the pump connected to COM1 If no key is pressed then K is the null string nothing is displayed or transmitted and the program continues execution Line 70 uses the LOC file number function to determine if any ch
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