ÄKTAdesign Pump P-920
Contents
1. End 1 00m min Run 1 00ml min Pause 1 00ml min Hold 1 00ml min I Run 23 40mi min Set Concentration B 0 0 B 5 0 I Set Gradient Length Set Flow Rate Set Gradient Target 0 0 B 10 0 I Set Conc Gradient 0 0 B I Pump Wash 20ml min J Pump Wash 20ml min Washing Warning Pump paused Pressure at 3 ml min Pump Sync 20ml min _ Pump Sync 20ml min _ Synchronizing pumps L Warning Pump paused Pressure at 3 ml min Change direction Change direction Check Check Piston Status A Left compressing Check Piston Strokes BH Canes ee A_123456789 B 3467 Check mixer run time 15 hours Check Service Mode Telephone Service 012345678901 Contract Number Setup Setup Hi ae tet Serial Number Setup Lo Press Limit Pump P 920 ee Press Lim Date of Maintenance Setup Pressure Unit Buzzer Test Setup Gradient Base Setup Mixer Q Setup Pulsecomp Setup Press Offset D Note Zero pressure Alarm Timer 12 30 52 __ Alarm Timer action Setup language P GATE ME Setup Unit number Calib Press Offset Set Timer S2. csssssscssssesssessssesessssesscseecscseseaes 49 Appendix D Accessories and Consumables Appendix E Short Instructions About this manual This manual comprises 3 parts a practical part sections1 5 a reference part sections A D and a quick guide section E Sections 1 5 contain the necessary information for installing operating and maintaining the instrument Pump P 920 User Manual 18 1125 54 Edition AF Important user information All users must read this entire manual to fully understand the safe use of Pump P 920 Important Pump P 920 is intended for research use only and should not be used in any clinical or in vitro procedures for diagnostic purposes Pump P 920 should not be used in any clinical or in vitro procedures for diagnostic purposes Safety notices This manual contains warnings and cautions concerning the safe use of the product See definitions below WARNING The WARNING symbol and notice highlight instructions that must be followed to avoid personal injury Do not proceed until all stated conditions are clearly understood and met CAUTION The CAUTION notice highlights instructions that must be followed to avoid damage to the product or other equipment Do not proceed until all stated conditions are met and clearly understood Notes Note A Note is used to indicate information that is important for trouble free and optimal use of the product Recycling This symbol i
3. Setup Press Offset Note Zero pressure Calib Press Offset Calib Press Offset Setup language Menus B B 2 5 Set mixer operation Sets the condition of the mixer connected to the pump 1 Select sub menu Setup Mixer press OK 2 Select either on off or auto press OK auto means the mixer is running when the pump is in Run Pause or Hold mode B 2 6 Set pulse compensation mode Sets the condition for pulse compensation 1 Select sub menu Setup Pulsecomp press OK 2 Select either on or off press OK B 2 7 Set pressure offset Allows pressure offset calibration 1 Select sub menu Setup Press Offset press OK 2 Awarning message is shown that the pressure must be zero in the pressure transducer to perform the calibration 3 Press OK to acknowledge or Esc to cancel When acknowledged the display returns to Setup Press Offset 4 Press OK to perform the calibration B 2 8 Setup language Sets the language used in the display 1 Select sub menu Setup Language and press OK 2 Select the desired language GB English D German F French E Spanish Italian Pump P 920 User Manual 18 1125 54 Edition AF 43 B Menus B 3 Alarm timer Setup Unit Number 0 Set Display Angle gt l gt gt l gt B 2 9 Setup unit number The unit number is the identification the pump has on the UniNet 1 communication link It should correspo
4. Inlet tubing B1 Mixer outlet 15 3 Operation 3 1 On off 3 Operation 3 1 On off Selftest Please wait Pump P 920 lt versionno gt End 0 00ml min 0 00MPa 0 0 B Switch on the module at the mains switch on the front panel At switch on the module performs a selftest Several beeps are heard during this process If an error is detected an error message is shown Name and software version number is shown for 2 seconds The selftest takes approximately 1 2 minute When start up is completed with no errors the display shows the main menu with the pump in End mode All parameters are factory set to default values 3 2 Menu selection and settings 16 Menu selection A specific menu is selected by turning the front selection dial clockwise or counter clockwise When the required menu is visible the menu or selection is accepted by pressing the OK button Menu selection OK button Esc button Go down one menu level Return one menu level or accept setting If a menu has sub levels the sub menu is displayed by pressing OK Pressing ESC moves back one menu level Return to higher menu levels Pressing ESC repeatedly returns to the main operating menu Turn the dial one click counter clockwise to return to the work mode changing menu Work mode menu Main operating menu Pump P 920 User Manual 18 1125 54 Edition AF Operation 3 Selecting a value A cursor below a text o
5. 1 Introduction General Pump P 920 is a high precision laboratory pump for use in liquid chromatography and other applications where constant flow is required The performance of Pump P 920 is accurate and reproducible from low flow rates over the whole pressure range The chemical resistance of the pump allows its use with corrosive liquids and organic solvents as well as aqueous solutions with high salt concentrations Pump P 920 facilitates routine chromatography work It is utilized as system pump in AKTAfplc chromatography systems or is used as a stand alone unit The flow rate is set locally with a front dial or from a PC running UNICORN 3 0 or higher Pump P 920 works with a wide range of columns and media supplied by GE Healthcare The pump can be used to form accurate and reproducible gradients In stand alone configurations setup parameters are stated in a Setup menu see reference information in section B 2 Pump P 920 features e A pressure sensor connected to pump module A and B e Pulse compensation 1 Introduction 1 2 Accessories 12 Accessories Mixer M 925 can be connected to the pump When P 920 is connected to UNICORN the mixer can be controlled on off auto from UNICORN When P 920 is run as a stand alone unit the mixer is operated via the local user interface 13 Safety e The module is designed for indoor use only e Do not use in a dusty atmosphere o
6. 4 9 Cleaning the 6 port pump valve 2 Clean the valve in place If improvement is not seen contact GE Healthcare for service 1 Replace the piston seal according to the instructions in section 4 7 Replacing the piston seal 1 Flush through to clear blockage 2 Ifnecessary replace tubing 3 Check inlet tubing filter It can become clogged if unfiltered buffers or samples are applied See instructions for flushing through at the end of the run in section 3 8 Ending the run and storage 1 Replace or re install the faulty part s 1 Disassemble the pump cylinder assembly and examine the piston seal and glass cylinder walls according to section 4 7 Replacing the piston seal Replace if necessary 2 Ifthe glass cylinder walls are scratched check the piston seal Ensure that the piston rinsing system is always used especially when working with aqueous buffers with high salt concentrations 3 Check the piston for damage If damaged replace the piston according to section 4 8 Replacing a damaged piston 4 Never reinstall used or old parts that may be worn 1 Unscrew the tubing connector and check if it is worn or incorrectly fitted If so replace the connector 2 Tighten the connector properly 1 Check the chart recorder according to its manual Pump P 920 User Manual 18 1125 54 Edition AF Trouble shooting 5 5 4 Error messages If the suggested actions do not correct the fault contact GE Healthcare 1 ERROR i
7. Replacing the piston seal Replacement instruction CAUTION Read the following instructions carefully Avoid fitting the individual parts of the pump cylinder assembly incorrectly Ensure that the orientation of each part is correct before continuing with the next instruction Setup Pulsecomp 1 Use operating menu Setup Pulsecomp to check that pulse compensation on onoff is off see Section B 2 6 If not select off Otherwise adjustment is not possible Root 2 Start the pump and run until the piston head reaches the middle of its cylinder Stop the pump by setting it to END work mode WN Rinsing tubing Switch OFF the pump at the mains power switch on the front panel 4 Remove the protective perspex cover by unscrewing the fixing screws 5 Remove the protective metal cover over the piston rod by gently pressing it together while pulling it off 6 Remove the rinsing tubing Also remove the tubing cover by undoing the Covers Phillips screws two Phillips screws Piston rod 7 Disengage the piston rod from the driving arm by loosening the stop screw stop screws with the 2 5 mm Allen key Unscrew the locking screw for the pump cylinder assembly in a clock wise direction and remove it Disconnect the inlet and outlet tubings from the pump cylinder assembly Unscrew the M3 nuts from the support rods Carefully r
8. are Run The pump is running with set flow rate End The pump is not running Flow rate and gradient are reset Pause The pump is stopped but the set flow rate and the gradient are retained Hold The gradient is held at the value displayed and the pump continues to run Continue Not a work mode Used to continue from Hold or Pause Work mode changing menu Work mode changes are made in the work mode changing menu above the main operating menu turn dial counter clockwise The current work mode is shown in the upper left corner of the display Available actions are shown at the lower right There are four different displays for this menu and the menu displayed will depend on the current mode see below When a new mode is selected the appearance of the menu will change Pressing OK in a work mode changing display selects the underlined work mode Different work modes are selected by turning the dial End 1 00ml min Pause 1 00ml min Hold 1 00ml min Run End Hold Continue End Continue Pause e Tostart the pump select Run and press OK e To stop the pump select End and press OK Pump P 920 User Manual 18 1125 54 Edition AF Operation 3 Preparation before starting CAUTION Before the start of each run always ensure that there is an adequate supply of eluent in the reservoirs Do not allow the pump to run dry since this will affect the lifetime of the sealings Check that there is suffi
9. pump modules A and B If the pump is stopped stopped is shown instead B 1 2 Checking the number of piston strokes 1 Select operating menu Check Piston Strokes and press OK 2 Thedisplay shows the accumulated number of piston strokes for the upper and lower pump cylinder assemblies B 1 3 Checking mixer run time 1 Select operating menu Check Mixer run time and press OK 2 The display shows the accumulated run time for the mixer B 1 4 Checking service mode Service information relevant to the module can be checked Information may not be available in all menus 1 Select operating menu Check Service Mode and press OK The service telephone number is displayed Press OK The service contract number is displayed Press OK The module serial number is displayed Press OK The module name and software version are displayed Press OK The date of the last service is displayed Press OK s OA OW FR WN N A test of the module buzzer is performed Press OK Not always pre programmed Depends on customer specific contract Pump P 920 User Manual 18 1125 54 Edition AF 41 B Menus B 2 Setup menu B 2 Setup menu Setup Hi Press Limit 5 00MPa 4 50 Setup Lo Press Limit 0 00MPa 1 00 Setup Wash Press Lim 5 00MPa 4 50 Setup Pressure Unit bar MPa bar psi Setup Gradient Base time time volume 42 Select main operating menu Setup and press OK to ac
10. sample injection valve blocked 36 Pump P 920 User Manual 18 1125 54 Edition AF Description A Appendix A Description A1 Module Pump P 920 is a high performance laboratory pump for use in liquid EE chromatography and other applications where constant liquid flow is required ov An eluent in an external vessel is drawn into the 6 port pump valve by the action a EZ of the pump cylinder assemblies Twin reciprocating piston heads work in unison to deliver a smooth low pulsation flow from the pump pressure sensor housing The module contains no internal user replaceable items A 2 Rear panel Pressure UniNet 1 gt 6 Mans x Voltage Frequency Power ma Fuse CEO 100 200v soson sova 163 at of the specified type and cur rent ratings Connector switch Function Pressure analogue out Oto 1V Pressure signal to chart recorder UniNet 2 Connection to Mixer M 925 and UniNet 2 network UniNet 1 Connection to UniNet 1 network Pump P 920 User Manual 18 1125 54 Edition AF 37 Description A3 Fluid delivery Connector switch Function Mains inlet with fuse Supply voltage grounded Mains outlets Supply voltage to UPC 900 Frac 920 and external units 0 1 Module on off switch on front panel Remote Input output for digital signals Auxiliary equipment with digital signal int
11. 1125 54 Edition AF Operation 3 3 8 Ending the run and storage Pump Wash 20 ml min Pump Wash 20 ml min AB B amp A Washing Please wait Overnight storage The pump can be left filled with a buffer Note f buffers or water are stored at room temperature bacterial growth may occur If no further runs are planned the pump should be washed immediately with pure eluent If aqueous buffers have been used washing with pure distilled water is particularly important to prevent salt precipitation Weekend and long term storage Wash the pump with water and then fill it with 20 ethanol Pump wash operation 1 Immerse the inlet filters in a wash solution Select operating menu Pump Wash and press OK 2 When the display shows Pump Wash A B and B amp A select B amp A and press OK 3 First pump B and then pump A is washed in sequence The pump wash is performed with 20 ml min for seven strokes each In UNICORN select instruction PumpWash in System Control Manual Pump In the Parameters window set PumpA and PumpB Click on Execute 3 9 Changing eluent CAUTION To prevent precipitation of crystals when changing from a salt containing buffer to organic solvent always wash the system with water as the intermediate liquid Note When changing from one eluent to another it is extremely important that the two eluents are totally miscible with one another If the two eluents are immiscible
12. 18 1125 54 Edition AF 29 4 Maintenance 4 8 Replacing a damaged piston 4 8 Replacing a damaged piston 1 Typical symptoms of a damaged piston are observed as excessive piston seal wear unstable pressure a reduction in the flow or insome cases noise as the piston moves The piston should be removed examined for damage or salt precipitation and then replaced with a new piston if necessary 2 Ifadamaged piston has been in operation the piston seal will be destroyed and should also be replaced To replace the piston and the seal follow the instructions in section 4 7 Replacing the piston seal 3 In addition to the spare parts listed in section 4 4 a pump head complete and possibly if scratches are found inside the glass cylinder a glass cylinder are also required see appendix D Accessories and Consumables for code numbers 4 9 Cleaning the 6 port pump valve 30 1 Faulty operation of the 6 way pump valve is usually indicated by no flow and that the pressure sensor gives no indication that the back pressure has increased The probable cause is that one or more channels are blocked 2 Identify the relevant pump cylinder assembly by observing which pump head is not delivering flow WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are being used Connect the tubing by first inserting the tubing fully then tightening the connector fin
13. 4 Edition AF 55 For contact information for your local office please visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Drop Design KTA and AKTAFPLC are trademarks of GE Healthcare companies UNICORN Any use of this software is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products All third party trademarks are the property of their respective owners 2004 2008 General Electric Company All rights reserved First published Jan 2004 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 18 1125 54 AF 06 2008
14. 55011 GR 2 Class A emission e This device complies with part 15 of the FCC rules emission Operation is subjectto the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation Pump P 920 User Manual 18 1125 54 Edition AF 49 C Technical Specifications C 3 Regulatory Requirements 50 Pump P 920 User Manual 18 1125 54 Edition AF Appendix D Accessories and Consumables Accessories and Consumables D Item Quant pack A C Code no Sealing kit containing two sealings 1 C 18 1032 16 two gaskets and two wipers Pump head complete 1 A 18 1032 15 Glass cylinder 1 A 18 1512 01 nternal tubing kit P 920 1 C 18 1128 70 Rinsing tubing 1 1 mm i d 3 1 mm o d 2m A 18 1032 11 Union 1 16 female M6 male PEEK 6 A 18 1112 57 Fingertight connector 1 16 10 A 18 1112 55 for PEEK tubing o d 1 16 Union 1 16 male 1 16 male 10 A 18 1120 92 for 1 16 o d tubing PEEK Stop plug 5 16 PEEK 5 A 18 1112 50 Stop plug 1 16 PEEK 5 A 18 1112 52 Tubing cutter 1 A 18 1112 46 U wrench M6 1 A 19 7481 01 U wrench 1 4 1 A 18 1112 45 Allen key 2 5 mm 1 A 19 4442 01 Signal cable 6 pin mini DIN open 1 A 18 1110 64 Chart Recorder REC 111 1 channel 1 A 18 1132 32 Chart Recorder REC 112 2 channel 1 A 18 1132 33 1 I A accessories C consumables P
15. GE Healthcare AKTAdesign Pump P 920 User Manual KTA Contents 1 Introduction Did Generdl 5355 ns necessite 1 2 Accessories 13 Safety oseese irris R RAAR 2 Installation 2 1 Unpacking sisi 2 2 General precautions 2 3 Connecting electrical signal cables cssssesesesecseseeeeeeees 11 2 4 Connecting to UniNet 1 communication link e 12 2 5 Connecting to supply voltage ccccsessssseseseseseseseeteeseeseseers 12 2 6 RINSING tubing essee nennir iir ioei 12 2 7 Connecting the inlet and outlet tubing cccsececeeeesseeees 13 2 8 Running in the new pump sn 13 2 9 Installation of accessories 0 0 0 0 esesessceceetceeeeceesececscessececsceeeeeees 14 3 Operation 3 1 OM OFF aise nas ns en rats 16 3 2 Menu selection and settings ssssssccssesssssssesssessssesseeeeeseees 16 3 3 Menu OVErvieW nn nee nrsreneneesrsrsrereresrsrsee 17 3 4 Starting and stopping the PUMP cssssecesssesesssseseeeeessecees 18 3 5 Setting the flow rate and starting the pump cesseee 19 3 6 Setting concentration B ss 20 3 7 Running a simple gradient ccscscscsesssssssssssesssssecececseseseees 20 3 8 Ending the run and storage scssscsccesssssssssssesessseessseseeseees 21 3 9 Changing eluent eesssssssesesessccsesescsessssssssscsessssseseeseseseerees 21 3 10 Restart after power failure ccsccsscssssssssesesesessseeseseset
16. P 920 is equipped with covers to protect both the user and the pump from accidental damage A perspex cover is fitted over the fluid delivery side of the pump The perspex cover is fixed with screws Metal covers are located over the moving part of the piston rods out side the glass cylinders These covers are held in position by spring action Pump P 920 User Manual 18 1125 54 Edition AF 39 Description A 7 Using an external chart recorder A 7 Using an external chart recorder The facility for recording the actual pressure at the pump outlet can be used for recording the pressure during an overnight run and for checking the pump function for any abnormality The external chart recorder output for pressure is 0 to 1 V where 0 1 V corresponds to 1 MPa 40 Pump P 920 User Manual 18 1125 54 Edition AF Menus B Appendix B Menus B 1 Check menu CheckPistonStatus A Left compressing B Rightcompressing CheckPistonStrokes A 123456789 B2398 CheckMixerrun time 15 Hous CheckServicdViode Telephon ervice ContractNumber SerialNumber PumpP 920 Date of Maintenance BuzzerTest Select main operating menu Check and press OK to access the check sub menus B 1 1 Checking piston status 1 Select operating menu Check Piston Status and press OK 2 Thedisplay shows if Left and Right cylinder assemblies are compressing for both
17. ailed to set offset 86 ERROR in EEPROM Recalib all values 88 Not allowed current mode 3300 Error software notify instr support 3400 Error software notify instr support 3401 Error software notify instr support 3410 Error software notify instr support 3411 Error software notify instr support 3510 Error software notify instr support 3621 Error software notify instr support 3622 Error software notify instr support 3624 Error software notify instr support 34 Switch off the module and let it cool Clean or clear the air inlets Switch on the module and check that the fan is running Call service Reboot system If error remains call service Call service for recalibration Select other instruction or change mode End Run or Pause Reboot system If error remains notify instrument support Pump P 920 User Manual 18 1125 54 Edition AF 3600 Low lim gt hi lim 3605 Low lim gt hi lim Hysteresis 3610 Disconpressure then set analog V 3620 Error hardware notify instr suppor 3636 Value must be greater than 0 ERROR key OK ERROR key Esc ERROR key OK Esc ERROR number 100 ERROR number 109 113 ERROR number 120 121 ERROR number 106 108 ERROR number 118 Trouble shooting 5 Low pressure limi
18. al cables O Pressure Analogue out 0 1 V Remote UniNet 2 UniNet 1 Mains output Leakage current ma 3 5 mA o GE Voltage Frequency Power ma Fuse 100 240 V 50 60 Hz 600 VA T6 3 AL WARNING For continued protection against O risk of fire replace only with fuse of the specified type and current ratings O The sockets for electrical signals are located on the rear panel Connecting to chart recorder if used 1 Connect the chart recorder to the Mini DIN socket Pressure Analogue out 0 1 V using the cable supplied E Pin Signal 1 Pressure signal 2 Signal ground 3 6 Not used reserved for factory testing Note The signal cable is delivered with protective covers on each wire Do not remove the protective covers from unused connections as a short circuit may disturb the measurement 2 Set the recorder to 0 to 0 5 V input full scale 0 1 V corresponds to 1 MPa Pump P 920 User Manual 18 1125 54 Edition AF 11 2 Installation 2 4 Connecting to UniNet 1 communication link 2 4 Connecting to UniNet 1 communication link When used in AKTAdesign chromatography system the pump is controlled from a PC running UNICORN version 3 0 or higher via UniNet 1 cables CAUTION The mains power to the AKTAdesign chromatography system must be switched OFF before connecting the module t
19. ate pressure and eluents used The seals are regarded as consumables and are available as a sealing kit The wear of the piston seals is not covered by the warranty of the pump A typical lifetime is around 1000 run hours WARNING Always disconnect the power supply before attempting to replace any item on the module during maintenance WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are being used Connect the tubing by first inserting the tubing fully then tightening the connector fingertight CAUTION Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the module 4 1 Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Follow local rules and regulations when disposing of waste substances Pump P 920 User Manual 18 1125 54 Edition AF 23 4 Maintenance 4 2 Periodic maintenance 4 2 Periodic maintenance Interval Action see procedures below Daily General care Monthly Change rinsing solution 20 ethanol Every two months Leakage test When required Fixing leaking connections Replacing the piston seals Replacing a damaged pist
20. cess the setup sub menus B 2 1 Set pressure limit Sets high and low pressure limits When the limit is reached the pump is set in Pause mode a buzzer sounds and an error message Overpressure for ascending pressure or Underpressure for descending pressure is shown This message has to be confirmed by pressing OK 1 Select sub menu Setup Hi Press Limit press OK 2 Set the value press OK 3 Repeat steps 1 and 2 for Setup Lo Press Limit Note Low pressure limit cannot be set above High pressure limit and High pressure limit cannot be set below Low pressure limit The low pressure limit check is delayed at start up to allow for system pressure build up B 2 2 Set wash pressure limit Sets wash pressure high limit When the limit is reached the pump is set in Pause mode a buzzer sounds and an error message Wash overpressure is shown This message has to be confirmed by pressing OK 1 Select sub menu Setup Wash Press Lim press OK 2 Set the value press OK B 2 3 Set pressure unit Sets the pressure unit used in the display 1 Select sub menu Setup Pressure Unit press OK 2 Select either MPa bar or psi press OK B 2 4 Set gradient base Sets the base for the gradient to either time or volume 1 Select sub menu Setup Gradient Base press OK 2 Select either time or volume press OK Pump P 920 User Manual 18 1125 54 Edition AF Setup Mixer auto onoff auto Setup Pulsecomp on on off
21. cient solvent present for the run and that the solvent filter is fully immersed If the eluent is to be changed see section3 9 Changing eluent Note The pump may not work if the buffer vessels are sealed or if they are placed too far below the pump inlets Do not close the vessels off completely Place the buffer vessels on the workbench or on the buffer tray on top of AKTAfplc Check that there is rinsing liquid 20 ethanol in the rinsing system behind the piston heads Set the pressure limit Refer to section B 2 1 for reference information If the pressure limit is exceeded the pump is stopped Emergency stop Use the front power switch or disconnect the mains supply from the pump 3 5 Setting the flow rate and starting the pump Set Flow Rate 0 00ml min 2 50 Select operating menu Set Flow Rate and press OK Set the desired value and press OK Go to the work mode menu select Run and press OK To change the flow rate while the pump is running repeat steps 1 and 2 To stop the pump go to the work mode menu select End and press OK In UNICORN select instruction Flow in System Control Manual Pump Click on Execute and the pump will start Pump P 920 User Manual 18 1125 54 Edition AF 19 3 Operation 3 6 Setting concentration B 3 6 Setting concentration B Set Conc Gradient 0 0 B Set Concentration B 0 0 B 5 0 A percentage of eluent B can be set 1 Select operating m
22. ector Locking screw Outlet connector 18 19 Piston rod Rinsing tubing connector End piece Refit the pump cylinder assembly Check that the end piece rinsing tubing connector points towards you Connect the inlet and outlet tubings to the pump cylinder assembly Check the assembly alignment and the connections carefully to eliminate the risk of glass cylinders bursting when pressure is applied Tighten the locking screw for the pump cylinder assembly and pull the piston rod to the end piece WARNING Hands and fingers risk being squeezed between the piston driving arm and the pump housing when running the pump without the protective covers fitted 20 21 22 23 24 Switch on the pump with the mains power switch on the front panel After the selftest run the pump at a low flow rate so that the piston rod slips into the hole in the driving arm Continue to run the pump until the 6 port pump valve switches Just as the valve switches stop the pump Engage the piston rod in the driving arm by tightening the stop screw with the Allen key Fill the rinsing system with 20 ethanol and replace the rinsing tubing Replace the tubing cover the protective cover over the piston rod and the perspex cover Make sure that no capillary is stuck between covers and the pump housing Run in the new sealings as described in section 2 8 Running in the new pump Pump P 920 User Manual
23. emove the glass cylinder together with the end piece and gently pull out the piston Remove the piston seal using a needle Be careful not to damage the piston Inlet and i i i i ll outlet rear head during this operation Fit the new seal by hand connections 11 The wiper is removed by pressing it out from the rod Press a new wiper onto Glass cylinder the rod The hole on the wiper should point towards the pump head Piston seal CAUTION Do not push the piston at an angle to the cylinder and do not Piston head twist the piston Support rods RE 12 Wet the piston head with distilled water and push it into the glass cylinder Piston 13 Change the end piece gasket located between the end piece and the glass cylinder Use a spatula to remove the worn gasket Wiper 14 Place the flat edge of the support rod assembly on a flat surface o TO piece 15 Mount the end piece on the support rod assembly so the tubing connectors M3 nuts are parallel to the flat edge 28 Pump P 920 User Manual 18 1125 54 Edition AF Maintenance 4 16 Mount the glass cylinder with the piston and the wiper onto the end piece Check that the rinsing tubing connector points upwards 17 Make sure that both the end piece gasket and the wiper are in position Insert the two support rods into the holes in the end piece When the glass cylinder is properly seated tighten the two M3 nuts gently Inlet conn
24. enu Set Conc Gradient and press OK 2 When the display shows Set Concentration B press OK 3 Set the desired B value and press OK In UNICORN select instruction Gradient in System Control Manual Pump Set the desired B value 3 7 Running a simple gradient Set Conc Gradient 0 0 B Set Gradient Length 0 0min 8 0 Set Gradient Target 0 0 B 50 0 20 Gradients can be run in time or volume base The default is time base To change base see section B 2 4 for reference information 100 Gradient length gt Target concentration Current concentration 0 Start of gradient The gradient is run from current concentration to target concentration over a set time or volume The gradient can be set in any operating mode 1 Select operating menu Set Conc Gradient and press OK 2 When the display shows Set Concentration B turn the dial to select operating menu Set Gradient Length and press OK 3 Set the desired value and press OK 4 Turnthe dial to select operating menu Set Gradient Target and press OK Set the desired gradient target value in B and press OK If the pump is in work mode Run the gradient starts immediately 5 Atthe end of the set gradient time the pump continues to run at the target concentration In UNICORN select instruction Gradient in System Control Manual Pump in the Parameters window set Target and Length Click on Execute Pump P 920 User Manual 18
25. erface can be connected to the9 pole D SUB female REMOTE connector 5 V TTL signals only Pin Signal Function e5 ol o e2 ol 14 Digital input 1 4 Active status low or closed terminal to pin 5 SEd OV Signal ground 6 9 Digital output 1 4 Active status 5 V in reference to pin 5 When used in combination with UNICORN and the AKTAfplc strategy 4 digital inputs and 4 digital outputs can be handled via the REMOTE connector Pins 1 4 are used as inputs and pins 6 9 as outputs All input output signals are 5 V TTL signals with ground reference to pin 5 A 3 Fluid delivery Pumping action is provided by a stepper motor through a gear box and a ball screw to two alternating pistons mounted on the right hand side of the pump module The driving mechanism is located behind the side panel The pistons reciprocate in two high precision glass cylinders The pistons are driven so that one piston draws in solvent while the other expel solvent The change of piston direction is controlled by opto electronic switches at the end positions These switches also control the 6 port pump valve movement Each piston consists of three parts e head e sealing e connecting rod The pump cylinder assembly is resistant to corrosive fluids and is easy to disassemble when servicing 38 Pump P 920 User Manual 18 1125 54 Edition AF A 4 A 5 A 6 Description A 6 port pump valve The 6 port pump valve is a motorized rotary valve driven b
26. ers for both the A and B pump Select 50 B and run the pump at 20 ml min to flush out the used rinsing solution Allow the pump to perform a couple of strokes to remove as much rinsing solution as possible from the glass cylinders Stop the pump when the pistons are at the middle of the glass cylinders Refit the rinsing tubing to the lower cylinders in each pump module Add fresh rinsing solution to the lower cylinders by submerging the rinsing tubing open ends in rinsing solution and starting the pump Continue to run until approximately half of the cylinder chambers are filled Stop the pump Refit the rinsing tubing to the upper cylinders Run the pump and allow a couple of strokes to wash out as much contamination from the old rinsing solution as possible If required repeat steps 1 to 6 one or two times to ensure that all contamination is washed out 4 6 Testing leakage When using the Pump P 920 regularly a leakage test should be performed every second month When the pump is used intermittently a leakage test should be performed before every use After disassembly due to cleaning or repair a leakage test should also be performed before use Stop plug Leakage is tested by checking the pump pressure against a blocked outlet using distilled water and a 1 16 stop plug The test is carried out from the pump dial and display A chart recorder connected to the analogue pressure output can be useful to record the pressure readi
27. et Display Angle Calib Press Offset Set Clock Alarm Timer off 46 Pump P 920 User Manual 18 1125 54 Edition AF Technical Specifications C Appendix C_ Technical Specifications C 1 Flow rate range isocratic mode gradient mode Pressure range Pressure pulsation pH stability range Viscosity Flow rate reproducibility flow rate 0 5 to 10 ml min Gradient composition accuracy between turnings accuracy during turnings reproducibility Leakage Pressure sensor range offset error scale error Operating data 0 01 to 20 ml min in steps of 10 ul min 0 1 to 20 ml min in steps of 1 l min 0 to 5 MPa 50 bar 725 psi Max 6 dP P during pump stroke 1 to 13 1 to 14 lt 1 day exposure Max 5 cP for complete flow rate range At reduced flow rate 5 ml min 10 cP rsd lt 0 2 2 at 0 5 to 5 ml min and lt 5 MPa 2 at 0 5 to 5 ml min and 0 5 to 2 0 MPa rsd lt 0 5 at 0 5 to 20 ml min and lt 5 MPa lt 0 5 ml min pump module A and B each O to 7 MPa Max 0 05 MPa Max 2 Pump P 920 User Manual 18 1125 54 Edition AF 47 C Technical Specifications C 2 Physical data C 2 Physical data Delay volumes per pump cylinder assembly Inlet tubing Outlet tubing Fuse specification Control Degree of protection housing Wetted materials piston head piston rod glass cylinder pump seal 6 port valve Chemical resistance Power requirement a
28. gertight Finally tighten the internal connectors a further 1 4 turn using the key supplied CAUTION Change the solvent to distilled water and flush out all salt before flushing the 6 port pump valve First try to clean the inlet channel in place by disconnecting the inlet tubing and the tubing from the corresponding pump cylinder assembly Clean the channels in the valve by flushing nitrogen in reverse flow direction through the valve Observe the capillary markings when reconnecting the tubing If this does not correct the problem contact GE Healthcare for service Pump P 920 User Manual 18 1125 54 Edition AF Trouble shooting 5 5 Trouble shooting 5 1 General 5 2 Recycling When contacting GE Healthcare for support state the version number of the module This is shown for 2 seconds during start up The version and model can also be checked by using the Check Service Mode operating menu We recommend you make a pressure recording since much information can be gained from the pressure trace See section 5 5 Checking the pump pressure for more information WARNING The module must not be opened by the user It contains high voltage circuits that can give a lethal electric shock This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufact
29. ime unit 4 If you want to set a count down time turn the dial to select sub menu Set Timer Press OK to enter the count down value in the form HH MM SS Press the OK button after entering each time unit 5 Press ESC button to return to the Alarm Timer menu which now shows the set alarm time or count down time as BuzzerHH MM SS 6 When the alarm time is due or the count down timer reaches 00 00 00 an alert display is shown and the module beeps until the OK button is pressed Asecond alert display is shown until the OK button is pressed Pump P 920 User Manual 18 1125 54 Edition AF Menus B Set Clock The alarm timer is based on the internal module clock which can be set in the Set Clock menu located after the Alarm Timer menu The clock will be reset when power is turned OFF A set alarm timer function can be reset by pressing OK in the menu Alarm Timer off Alarm Timer off B 4 Service displays The module has service displays for use by authorized service personnel If the service display Enter Access Code is accidentally selected press the ESC button to return to the normal operating menus Enter Access Code Pump P 920 User Manual 18 1125 54 Edition AF 45 B Menus B 5 Menu and text overview B 5 Menu and text overview Turn Dial to select menus on same level Press OK to acknowledge selection Press Esc to move up one menu level Press Esc again to move up further
30. lected Pump synchronization selection This means that the pump cylinder s are run to one stop position Individual pump cylinder assemblies or a complete pump synchronization can be selected Change piston direction selection Individual piston direction or both piston directions can be selected Check internal operating values See reference information in section B 1 Setup language pressure limits and unit etc See reference information in section B 2 Set different timer options The pump can be started or stopped at set times See reference information in section B 3 Pump P 920 User Manual 18 1125 54 Edition AF 17 3 Operation 3 4 Starting and stopping the pump 3 4 Starting and stopping the pump Run 13 40m min 2 00MPa 45 5 B Run 1 00ml min End Hold Pause Current Available work actions for mode the current work mode 18 WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are being used Connect the tubing by first inserting the tubing fully then tightening the connector fingertight WARNING All protective covers over internal capillaries piston rods and glass cylinders must be in place when running the pump Main operating menu The main operating menu shows the current flow rate together with work mode indication pressure and B if used The available work modes
31. n software notify instr support 33 ERROR in software notify instr support 34 ERROR in software notify instr support 35 ERROR in software notify instr support 70 WARNING no mixer Check connection 71 Error in mixer Check mixer 72 Error in UniNet2 Checkunits 73 WARNING UniNet2 Check connection 75 Error in valve A Cont Call service 76 Error in valve B Cont Call service 80 WARNING Pump overpressure 81 WARNING Pump underpressure 82 WARNING pump overheated check fan 1 2 WN N Reboot system If error remains notify instrument support Switch off the module Check the UniNet 2 connections to the mixer Check the mixer Switch on the module Switch off the module Check all UniNet 2 connections Switch on the module Switch off the module Switch on the module If still persistent call service Check high pressure limit setting Check the column may be blocked Check the flowpath for blockage Check low pressure limit setting Check the column may be leaking Check the flowpath for leakage Check for air in the pump Pump P 920 User Manual 18 1125 54 Edition AF 33 5 Trouble shooting 5 4 Error messages 80 WARNING Pump overpressure 81 WARNING Pump underpressure 83 ERROR endsensors pump A call service 84 ERROR endsensors pump B call service 85 F
32. nd to the number set in UNICORN for the pump The number should be set to 0 if one pump is used If more than one pump is used they must all have different identification numbers Factory default setting 0 1 Select sub menu Setup Unit Number press OK 2 Select unit number 0 25 press OK B 2 10 Setup display angle Sets the display angle to compensate for different viewing heights 1 Select main menu Setup press OK 2 Select sub menu Set Display Angle press OK 3 Select display angle gt Up gt Mid or gt Down press OK B 3 Alarm timer Alarm Timer 12 30 52 Alarm Timer Action Set Alarm 12 32 21 Set Timer Alarm Timer 12 30 52 12 41 29 12 41 49 44 You can set the alarm function to either a fixed alarm time or use a count down timer The pump can be started or stopped automatically or an alarm can sound at the set time It is not possible to set both an alarm time and the count down timer Current values are shown in parentheses 1 Select main menu Alarm Timer press OK 2 Set the action to take place Press OK to select action Buzzer will generate an audible alarm for 15 s with a message Run will start the pump at the set flow rate End will stop the pump Each generates one beep and a message 3 Use the sub menu Set Alarm if you want to set an alarm at a fixed time Press OK to enter the time in the form HH MM SS Press the OK button after entering each t
33. ndicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment WARNING This is a Class A product In a domestic environment it might cause radio interference in which case the user might be required to take appropriate measures WARNING All repairs should be done by personnel authorized by GE Healthcare Do not open any covers or replace parts unless specifically stated in the instructions CE certification This product complies with the European directives listed below by fulfilling corresponding standards A copy of the Declaration of Conformity is available on request e 73 23 EEC Low Voltage Directive e 89 336 EEC EMC Directive The CE logo and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used inthe same state as it was delivered from GE Healthcare except for alterations described in this manual Note The Declaration of conformity is valid only for systems that are marked with the CE logo CE 1 1 1 Pump P 920 User Manual 18 1125 54 Edition AF Introduction
34. ng during the test Pump P 920 User Manual 1 Set the pump to 0 B and select a flow of 20 ml min 2 Set the high pressure limit to 4 5 MPa and press OK 3 Run the A pump until the piston which is delivering flow is at the middle of the glass cylinder Stop the pump 4 Plug the pump outlet at the pressure manifold using a 1 16 stop plug 18 1112 52 5 Select a flow of 0 2 ml min 8 1125 54 Edition AF 25 4 Maintenance 4 6 Testing leakage 26 10 11 Start the pump and let it run until the pressure limit is reached and the pump stops Check the pressure reading for two minutes The pressure must not decrease more than 0 45 MPa during this time If the decrease is larger see Chapter 5 Trouble shooting for actions Release the pressure from the pressure manifold by loosening the stop plug Select a flow of 20 ml min and run the A pump until the other piston which is now delivering flow is at the middle of the glass cylinder Stop the pump Repeat steps 4 to 7 Set the pump to 100 B and select a flow of 20 ml min Release the pressure from the pressure manifold by loosening the stop plug Run the B pump until the piston which is delivering flow is at the middle of the glass cylinder Stop the pump Repeat steps 4 to 7 Repeat steps 8 for the B pump Repeat steps 4 to 7 Remove the stop plug and reconnect the outlet tubing to the pressure manifold S
35. o the power outlets on the back of P 920 The maximum permissible load 500 VA The leakage current may not exceed 3 5 mA gt gt gt e gt Pump P 920 User Manual 18 1125 54 Edition AF 9 2 Installation 2 1 Unpacking 2 Installation 2 1 Unpacking Unpack the module and check the items against the supplied packing list Inspect the items for obvious damage that may have occurred during transportation We recommend that all packing materials be retained if onward transport of the module is expected CAUTION Read the following information carefully to ensure that the module is installed correctly 2 2 General precautions 10 The module should be installed in a non corrosive atmosphere The module should be located in a place of low temperature variations away from heat sources draughts and direct sunlight Ventilation openings should not be blocked The module may be operated at normal ambient temperatures in the range 4 C to 40 C The module should be installed on a stable laboratory bench or in an AKTA design chromatography system To ensure correct ventilation a free space of 0 1 m is required behind and in front of the module Place the module directly on the bench To ensure that the ventilation inlet below the front is not blocked do not place soft material under the module Pump P 920 User Manual 18 1125 54 Edition AF Installation 2 2 3 Connecting electrical sign
36. o the UniNet 1 link 1 Connect two UniNet 1 cables to the UniNet 1 connectors e The module can be connected in series anywhere in the chain between the PC and the termination plug e The UniNet 1 link connects in series the PC with Pump P 920 and other modules e The termination plug is connected to the last module in the chain 2 5 Connecting to supply voltage 1 Make sure the on off switch is in the OFF position marked O 2 Connect a mains cable between the module and a grounded mains socket Any voltage from 100 to 240 V AC 50 to 60 Hz can be used WARNING The module must be connected to a grounded mains socket The module contains one user replaceable fuse See Technical Specifications for fuse ratings 2 6 Rinsing tubing Pump rinsing tubing I AT 7 DS Ce D AD RS ey CALS Pump rinsing tubing The rinsing tubing for the piston seals is installed at the factory and filled with 20 ethanol Check that the rinsing solution is present behind the pistons and that the rinsing tubing is connected and undamaged Always use 20 ethanol as rinsing solution Change rinsing solution at least every month 12 Pump P 920 User Manual 18 1125 54 Edition AF 2 7 2 8 Installation 2 Connecting the inlet and outlet tubing Inlet pump Inlet pump module A module B p Outlet to mixer In AKTAfplc the inlet and outlet tubing are in
37. on Cleaning or replacing 6 port pump valve 4 3 Pump cleaning Perform a pump wash using a cleaning or sanitizing agent The standard recommendation is to use 1 M NaOH and then wash out with buffer or distilled water WARNING NaOH is injurious to health Avoid spillage 4 4 General care Inspect the pump daily for eluent leaks Grange aieeuion If at any time air bubbles are trapped in a cylinder assembly purge the pump AB A amp B by manually changing the direction of the piston when the piston reaches its end position Change direction several times until the air bubbles are removed General recommendations for all eluents It is essential that all liquids passing through the pump are clean pure and degassed Impure or dirty eluents will not only cause baseline noise and drift but also block the channels in the 6 port pump valves shortening their lifetime Degassing prevents formation of air bubbles which can cause baseline noise drift and deteriorating gradient formation Additional recommendations for aqueous eluents After running with an aqueous eluent the pump should always be thoroughly washed with pure distilled water to prevent salt precipitation 24 Pump P 920 User Manual 18 1125 54 Edition AF Maintenance 4 4 5 Changing rinsing solution The rinsing solution should be changed monthly Use a 20 solution of ethanol distilled water 1 Remove the rinsing tubing between the glass cylind
38. outlet in a waste container Snr 2 Switch the mains power switch on the front panel to ON A selftest is carried Please wait out Note When the pump is run for the first time after replacing a sealing friction between the glass cylinder and the dry sealing may give rise to some noise This will disappear when the sealing has been wetted 3 Leakage is tested by checking the pump pressure against a blocked outlet using distilled water and a 5 16 stop plug The test is carried out from the pump dial and display A chart recorder connected to the analogue pressure output can be useful to record the pressure reading during this test See section 4 6 Testing leakage for details 4 Remove the stop plug and reconnect the outlet tubing The pump is then ready for operation Before changing solvent see section 3 9 Changing eluent 2 9 Installation of accessories Install Mixer M 925 as follows Mount the attached mounting bracket with Use one of the mounting rails CAUTION Before connecting the mixer M 925 make sure the power switch is in OFF position 14 Pump P 920 User Manual 18 1125 54 Edition AF Remote UniNet 1 Pressure Analogue out 0 1 V Q Mains autant Inlet tubing A1 Outlet tubing Pump P 920 User Manual 18 1125 54 Edition AF Stop plug Installation 2 The mixer is connected to the UniNet 2 connector
39. r close to spraying water e Do not block the air inlet and outlet of the unit WARNING The module must be connected to a grounded mains socket WARNING Always disconnect the power supply before attempting to replace any item on the module WARNING The module must not be opened by the user It contains high voltage circuits that can give a lethal electric shock WARNING When using hazardous chemicals all suitable protective measures such as protective glasses must be taken WARNING NaOH is injurious to health Avoid spillage gt gt gt gt e 8 Pump P 920 User Manual 18 1125 54 Edition AF Introduction 1 WARNING Incorrectly fitted tubing may loosen causing a jet of liquid to spray out This is especially dangerous if hazardous chemicals are being used Connect the tubing by first inserting the tubing fully then tightening the connector fingertight WARNING During normal operation all protective covers over internal capillaries piston rods and glass cylinders must be in place when running the pump WARNING Hands and fingers risk being squeezed between the piston driving arm and the pump housing when running the pump without the protective covers fitted WARNING The power switch shall always be easily accessible so that the unit can easily be disconnected in the event of an emergency WARNING Only GE Healthcare approved products may be connected t
40. r numerical value shows what is affected by the dial To increase the value turn the dial clockwise To decrease the value turn the dial counter clockwise The value can be reset by turning the dial several clicks counter clockwise Parameter Setup Hi Press Limit 5 00MPa 5 00 Current value E New value to be set To simplify entering large numerical values the cursor moves up to the next digit if the dial is turned quickly in one direction The cursor moves back one place to the right every two seconds if the dial is not turned The text or numerical value displayed is accepted by pressing OK To cancel press ESC 3 3 Menu overview End 1 00ml min Run Run 13 40m min 2 00MPa 45 5 B Set Flow Rate 0 00ml min Set Conc Gradient 0 0 B Pump Wash20ml min Pump Sync20ml min Change Direction Check Setup Alarm Timer12 30 52 Work mode changing menu From here the pump is started stopped held paused and continued This menu is accessed from all positions by turning the dial one click counter clockwise The appearance of this menu depends on the current mode Main operating menu The menu is accessed from all positions by pressing ESC repeatedly Setting flow rate in ml min Setting concentration and gradient values Wash program selection Individual pump cylinder assemblies or a complete pump wash can be se
41. seeees 22 4 Maintenance GD ROCY CHING cissssescccccsccisccesesccteinscscsccesascsussntetessesisesivesbsovansessindvcastsestees 23 4 2 Periodic maintenance sen 24 4 3 PUMP cleaning sn 24 44 General CO Eroen cavacsesdeiasdssasescveunsetensnsesesasteesevassessensesesastsesedndts 24 4 5 Changing rinsing solution sscssesessseceesesseseseescececeesesesees 25 4 6 Testing leakage Pump P 920 User Manual 18 1125 54 Edition AF 3 4 7 Replacing the piston Seal ccsssscesssesssesessesesecscsssescecseseeees 4 8 Replacing a damaged piston cssssssssssssessssssessscscececessessaees 4 9 Cleaning the 6 port pump valve 5 Trouble shooting 5 1 General isc ccssncusevccces scevcavesies een 5 2 Recycling ce 5 3 Faults and actions 5 4 Error MOSSAGES tn nn T 5 5 Checking the pump pressure Appendix A Description Al Module et E E A 2 Rear panel A 3 Fluid delivery A4 6 port PUMP valve nes A5 Pressure monitoring A 6 Protective COVES rennes A 7 Using an external chart recorder sssssssssssssssssssssscsescsesees 40 Appendix B Menus Bele CHECK MENU ssssssnnnssenintsnsnsiinnnn n ssh B 2 2SQEUP MENU is nee eenen renees eree E EEO ERS Eoi se sae B 3 Alarm timer B 4 Service displays B 5 Menu and text overview ren 46 Appendix C Technical Specifications C1 Operating datae riria 47 C2 Physical data naniesienia 48 C 3 Regulatory Requirements
42. stalled at the factory When using P 920 in stand alone applications the inlet and outlet tubing must be installed The outlet tubing PEEK tubing i d 0 50 mm o d 1 16 has tubing connectors attached at both ends The inlet tubings A1 B1 teflon tubing i d 1 6 mm o d 1 8 have tubing connectors attached at one end and inlet filters at the other end The inlet filters have replaceable filter inserts The inlet tubings are connected to the inlets marked A IN and B IN on the pump valves and the outlet tubing is connected to the upper connection on the pressure sensor marked OUT The other end of the outlet tubing is connected to a mixer inlet Running in the new pump General To be able to follow this section you must know how to operate the pump Read through section 3 Operation before continuing Note Running with air is not harmful to the pump sealings However the sealing ring lifetime might be shortened if the pump pumps air for long periods The pump is tested at the factory prior to delivery and washed with20 ethanol Remains of this is removed by running in the pump with a liquid such as distilled water that is miscible with ethanol After running in the pump is primed for the chromatographic run Pump P 920 User Manual 18 1125 54 Edition AF 13 2 Installation 2 9 Installation of accessories Running in 1 Submerge the inlet tubings in a suitable reservoir filled with degassed distilled water Place the
43. t can not be set higher than high pressure limit Reset limit Low pressure limit for warning can not be set higher than high pressure limit for warning Overlapping ranges due to hysteresis are not allowed Reset limit Release pressure in system before setting analogue output voltage Call instrument support Full scale pressure must be greater than zero Reset value The key was pressed during self test or is faulty Switch off the module Switch on the module Switch off the module Check all connections Switch on the module Switch off the module Check all UniNet 1 and UniNet 2 connections Switch on the module Pump P 920 User Manual 18 1125 54 Edition AF 35 5 Trouble shooting 5 5 Checking the pump pressure 5 5 Checking the pump pressure To check the pump function make a recording of the pressure on a chart recorder or check the pressure in UNICORN This pressure recording is more sensitive than the reading on the display By observing the piston running indicator in Check menu see section B 1 1 together with the pressure trace the pump cylinder assembly which is functioning abnormally can be identified There can be several causes of an abnormal pressure recording for example e partially blocked solvent filters e leaking connections e piston seal leakage e 6 port pump valve malfunction e piston and glass cylinder damaged e flow restrictor blocked e column blocked e mixer blocked e
44. the pump should be washed first with an intermediate liquid that is miscible with both eluents Failure to do this will disrupt the flow from the pump When changing from a salt containing buffer to an organic solvent use water as the intermediate liquid to prevent precipitation Pump P 920 User Manual 18 1125 54 Edition AF 21 3 Operation 3 10 Restart after power failure 1 Stop the pump by setting it in Pause mode If not carried out from UNICORN use a bypass tubing to replace the column Transfer the inlet tubing into the new eluent or into the intermediate liquid Perform a pump wash n FP NWN N If an intermediate liquid is being used transfer the inlet tubing into the final eluent and repeat step 4 with the new eluent In UNICORN select instruction PumpWash in System Control Manual Pump In the Parameters window set PumpA and PumpB Click on Execute Note When high buffer purity is important use an intermediate step This avoids remains of the previous buffer in the inlet filter diffusing out in the new buffer 3 10 Restart after power failure If the power supply to the pump is interrupted it automatically restarts after performing a selftest when power is restored All values under the Setup menu are retained Other operating values for example flow rate are reset 22 Pump P 920 User Manual 18 1125 54 Edition AF Maintenance 4 4 Maintenance Note The piston seals have a limited lifetime depending on the flow r
45. tore the pressure recordings in the system logbook Pump P 920 User Manual 18 1125 54 Edition AF 4 7 Maintenance 4 Replacing the piston seal If there are signs of leakage between piston seal and glass cylinder replace the piston seal of the leaking pump cylinder A seal leakage will gradually fill up the rinsing chamber completely causing excess fluid to leak out around the rinsing tubing connections CAUTION Do not disassemble the pump mechanism unless there is good reason to believe that the seal is leaking Always ensure that sufficient spare components are available before attempting to replace the piston seal We do not recommend refitting a used piston seal after removal Note Before disassembling the pump mechanism move all input buffer bottles to below the level of the pump pistons to prevent siphoning Note Always replace the piston seals on both piston heads of a pump module at the same time Required spare parts and tools Seal kit containing see appendix D Accessories and Consumables for code numbers e Piston seal kit containing two piston seals two gaskets and two wipers e M6 wrench included in AKTAfplc tool kit e 2 5mm Allen key included in AKTAfplc tool kit e U wrench NV 5 5 included in AKTAfplc tool kit Note After new sealings have been installed check the pump for leakage See section 4 6 Testing leakage Pump P 920 User Manual 18 1125 54 Edition AF 27 4 Maintenance 4 7
46. ump P 920 User Manual 18 1125 54 Edition AF 51 D Accessories and Consumables 52 Pump P 920 User Manual 18 1125 54 Edition AF IH fr ILE Selftest Pump P 920 End 0 00 ml min Set Flow Rate 0 00ml min 2 5 End 2 50ml min Run Run 2 50 ml min 2 0 MPa 0 0 B Set Conc Gradient 0 0 B 10 0 Set Gradient Length Set Gradient Target 0 0 B 10 0 End 2 50ml min Run Short Instructions E Short Instructions The following short instructions are intended as a guide to users who are fully familiar with the safety precautions and operating instructions described in this manual These short instructions assume that the module is installed according to the installation instructions ms a 3 N Select OK ESC a 1 Switch on the module with the mains switch on the rear panel 2 Aself test is performed 3 The version number is shown for two seconds 4 The main operating menu is shown 5 Set the flow rate 6 Start the pump by setting it in RUN mode 7 The main operating menu is shown 8 Set concentration and gradient 9 Start a gradient by setting the Gradient Length and Gradient Target 10 Stop the pump by setting it in END mode Pump P 920 User Manual 18 1125 54 Edition AF 53 E Short Instructions 54 Pump P 920 User Manual 18 1125 54 Edition AF Pump P 920 User Manual 18 1125 5
47. urer for information concerning the decommissioning of equipment Always follow local rules and regulations for the disposal of waste materials and substances 5 3 Faults and actions Fault If the suggested actions do not correct the fault contact GE Healthcare Action Large spillage over into system module 1 Unplug the mains inlet cable 2 Clean and dry the system module with a dry cloth or paper If necessary tilt the system module backwards to drain 3 Contact GE Healthcare local service for advice No text on the front display 1 Check that the mains cable is connected and the power switch is in ON position 2 Check the mains power supply 3 Check the mains inlet fuse Pump P 920 User Manual 18 1125 54 Edition AF 31 5 Trouble shooting 5 3 Faults and actions Fault Action Erratic flow noisy baseline signal irregular pressure trace Air bubbles passing through or trapped in the pump 6 port pump valve not functioning correctly Piston seal leaking Blockage or partial blockage Liquid leaking from the assembly or worn Wiper and or end piece gasket incorrectly fitted pump cylinder Low eluent flow and noise as the pistons move Leaking connection and or crystallized material around a connector Error in external chart recorder 32 1 Check that there is sufficient eluent present in the reservoirs 2 Check all connections for leakage 1 Follow the instructions in section
48. x Power consumption Digital input 5 V Digital output Recorder output Functions Display Environment Dimensions H x W x D Weight 48 10 ml Tubing connector for 1 8 o d tubing Fingertight connector 1 16 o d T 6 3 AL 250V Stand alone or from a PC running UNICORN version 3 0 or higher via UniNet 1 cable connection IP 20 PEEK Titanium Borosilicate glass Simriz PEEK titanium and ruby sapphire The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules 100 to 240 V AC 50 to 60 Hz 600 VA including accessories TTL low or contact closure see section A 2 for pin connections TTL open collectors 0 to 1 V full scale 0 1 V corresponds to 1 MPa Languages available English German Spanish French Italian 2 rows with 20 characters each 4 C to 40 C 20 to 95 relative humidity 84 to 106 kPa 840 to 1060 mbar 150 x 260 x 370 mm 18 kg Pump P 920 User Manual 18 1125 54 Edition AF Technical Specifications C C 3 Regulatory Requirements Safety standards This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC through the following harmonized standards e EN 61010 1 e IEC 61010 1 e CAN CSA C22 2 No 61010 1 e UL61010 1 EMC standards This device meets the requirements of the EMC Directive 89 336 EEC through the following harmonized standards e EN 61326 emission and immunity e EN
49. y a DC motor through a gear box The function of the valve is to control the solvent flow to and from the cylinder assemblies The valve has six tubing connections The 6 port pump valve operates automatically when the pistons reach their end positions The end positions are detected by optoelectronic switches The valve is also operated when manual change of direction is ordered via the front dial In stand alone applications all connections except IN and OUT are fitted at the factory When used in AKTAfplc chromatography systems the IN and OUT connections are also connected at the factory Pressure monitoring The pressure generated by the pump is continuously monitored by a pressure transducer housed in an outlet manifold block The pressure transducer generates a signal which is proportional to pump pressure This signal is read by UNICORN via the UniNet 1 connection and is displayed on the front panel display It is also fed to the rear panel connector Pressure analogue out 0 1 V where 0 1 V corresponds to 1 MPa The pressure transducer has two main functions e to continuously measure the operating pressure e to sense excessive pressure that could damage the columns If the pressure builds up to the set limit the pump automatically shuts down and an alarm is given Protective covers WARNING All protective covers over internal capillaries piston rods and glass cylinders must be in place when running the pump Pump
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