x - American Instrument Exchange, Inc.
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1. 7000 GA 24 Outer Row and GA 10 GA 24 Inner Row Rotor Speed At Fmax At tmax rpm 123 mm 108 mm 6400 5640 4950 6000 4960 4350 5500 4170 3660 5000 3440 3020 4500 2790 2450 4000 2200 1940 3500 1690 1480 3000 1240 1090 2500 861 753 2000 551 483 1500 309 272 1000 137 120 6000 4000 amp GA 10 5 2000 0 1000 2000 3000 4000 5000 6000 RPM 6000 4000 _ Outer Row GA 24 2 Imax 123 mm e Inner Row Tmax 108 mm E 2000 0 1000 2000 3000 4000 5000 6000 RPM 7000 GA 10 and GA 24 Rotors DESCRIPTION These Beckman Coulter rotors have been manufactured in an NSAI registered ISO 9001 or 9002 facility for use with the appropriately classified Beckman Coulter centrifuge The GA 10 and GA 24 rated for 6400 rpm are fixed angle rotors designed to centri fuge up to 10 GA 10 or 24 GA 24 containers at a 35 degree angle to the axis of rotation Used in Beckman Coulter GS 6 Spinchron and GP series centrifuges the rotors develop centrifugal forces that can efficiently pellet bacterial cells from large volumes or particles from tissue homogenates Short column methods such as partially fi2. Subject to the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions in effect at the time of sale Beckman Coulter agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of a benchtop centrifuge rotor to the orig inal buyer by Beckman Coulter or by an authorized represen tative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge all centrifuge parts required for repair CONDITIONS 1 Except as otherwise specifically provided herein this warranty covers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor characteristics or operated in a Beckman Coulter centri fuge that has been improperly disassembled repaire
3. see Table 7 Weight of fully loaded rotor 0000 6 4 kg 14 1b Rotor material uas rora ter ee eee eee aluminum lt lt min E a av gt The critical speed range is the range of speeds over which the rotor shifts so as to rotate E about its center of mass Passing through the critical speed range is characterized by Pa ie max a some vibration Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed rw to the standard acceleration of gravity g according to the following formula Axis of Rotation RCE 12 g where r is the radius in millimeters w is the angular velocity in radians per second 2 RPM 60 and g is the standard acceleration of gravity 9807 mm s2 After substitution 2 RCF 1 12r e The GA 24 rotor should always be run with the LOW brake setting to prevent sample mixing GA 10 and GA 24 Rotors Table 1 Relative Centrifugal Fields for GA 10 and GA 24 Rotors Entries in the table are calculated from the formula RCF 1 12 r RPM 1000 2 and then rounded to three significant digits Relative Centrifugal Field x g
4. 120 grams heaviest load in grams GA 10 and GA 24 Rotors GA 24 speed 6400 rpm 36 grams ws heaviest load in grams Heaviest single cavity load including tube and cap e When 50 mL glass tubes are run in the GA 10 reduce rotor speed to 1000 rpm to avoid breakage no adapters are used with these tubes e When certain glass tubes made by other manufacturers are used reduce rotor speed to prevent tube breakage Tables 6 and 7 indicate recommended g force limits to minimize breakage and are intended as guides only Because the strength of other manufacturers glass and plastic tubes can vary from lot to lot and will depend on handling and usage Beckman Coulter highly recommends that you pretest these tubes in the appropriate labware in these rotors using water samples to determine optimal operating conditions REMOVAL AND SAMPLE RECOVERY AN CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories 1 Remove the rotor lid and unload the containers 2 If removing the rotor use the torquing bar to release the tie down screw then lift the rotor straight up and off the drive shaft GA 10 and GA 24 Rotors Table 6 Recommended g Force Limits to Minimize Breakage of Certain Glass Tubes and Bottles Made by Other Manufacturers in the GA 10 Rotor Volume Dimensions Sa
5. Villepinte France 95942 ROISSY CDG Cedex Telephone 01 49 90 90 00 Fax 01 49 90 90 10 e mail bioresearch france beckman com GERMANY Beckman Coulter GmbH Siemensstrasse 1 D 85716 Unterschleissheim Lohhof Germany Telephone 89 35870 0 Fax 89 35870 490 e mail bioresearch de beckman com Ozons COULTER Beckman Coulter Inc HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 2814 0481 Fax 852 2814 1599 2873 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 02 953921 Fax 02 95392264 e mail beckman_italia beckman com JAPAN Beckman Coulter K K Toranomon 37 Mori Bldg 3 5 1 Toranomon Minato ku Tokyo 105 0001 Japan Telephone 03 5404 8395 Fax 03 5404 8436 MEXICO Beckman Coulter de Mexico Calle Adolfo Prieto 1213 Colonia Del Valle Mexico D F C P Mexico 03100 Telephone 525 559 16 35 Fax 525 559 36 12 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 0297 230630 Fax 0297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 331 North Bridge Road Odeon Towers 07 01 02 Singapore 188720 Telephone 65 339 3633 Fax 65 336 6303 SOUTH AFRICA Beckman Coulter Stand 1A Fedsure Park
6. cap separate 11x39 1 8 white polyethylene 340196 344497 GA 10 29 x 103 50 polypropylene 357005 none GA 10 29 x 103 50 polycarbonate 363664 none GA 10 Continued Table 4 Beckman Coulter Tubes and Bottles for GA 10 and GA 24 Rotors continued Tube Dimensions Volume Part mm mL Description Number Adapter Rotor Bottles 16 x 80 10 polycarbonate 355672 342327 GA 10 with cap 29 x 104 50 polycarbonate 357001 none GA 10 with cap assembly 29 x 104 50 polycarbonate 357002 none GA 10 with screw cap 29 x 104 50 polycarbonate 361693 none GA 10 with cap assembly 29 x 104 50 polyallomer 357003 none GA 10 with screw cap PREPARATION AND USE Specific information about the GA 10 and GA 24 rotors is given here Information about the centrifuge is contained in the centrifuge operator s manual which should be used together with this manual for complete rotor and centrifuge operation gt NOTE Although rotor components and accessories made by other manufacturers may fit in these rotors their safety in these rotors cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the GA 10 or GA 24 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor GA 10 and GA 24 Rotors GA 10 and GA 24 Rotors PREPARATION A
7. Beckman Coulter Inc This RGA form may be obtained from your local Beckman Coulter sales office and should contain the following information e serial number e history of use approximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible e name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and e name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Sterilization and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the outside of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office GA 10 and GA 24 Rotors 18 SUPPLY LIST
8. no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included in each box of tubes or bottles for tube and bottle sterilization and disinfection procedures TUBE BREAKAGE If a glass tube breaks remove the glass very carefully If all the glass particles are not contained in the rotor thoroughly clean the interior of the centrifuge chamber bowl and the underside of the gasket including the groove where it contacts the bowl the rotor and lid and the adapters in use if any re Be careful when examining or cleaning the gasket and chamber as glass particles may be imbedded in their surfaces If all the glass is not removed from the chamber and gasket subsequent runs will cause the remaining glass fragments to be pulled into the chamber where they will abrade the surface of the bowl The procedure for removing glass particles from the centrifuge chamber and gasket is in your centrifuge manual Carefully remove any glass particles from adapters in which tubes have broken Scrub them with a brush to be sure they are clean 4 Flammability hazard Do not use in or near operating ultracentrifuges 16 GA 10 and GA 24 Rotors RETURNING A ROTOR Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from
9. 0 and GA 24 Rotors 10 Table 5 Approximate Acceleration and Deceleration Times for the GA 10 and GA 24 Rotors Acceleration Deceleration LOW brake setting gt NOTE seconds seconds Instrument Empty Loaded Empty Loaded 60 Hz Nonrefrigerated Centrifuge 65 70 55 60 60 Hz Refrigerated Centrifuge 65 70 55 60 50 Hz Nonrefrigerated Centrifuge 80 85 55 60 50 Hz Refrigerated Centrifuge 80 85 55 60 Line voltage fluctuations can cause variations in acceleration and deceleration times Values in Table 5 were achieved with line voltage ratings at 120 volts for 60 Hz instruments and 220 volts for 50 Hz instruments RUN SPEEDS The centrifugal force at a given radius in a rotor is a function of speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rotational speed is adjusted so that identical samples are subjected to the same RCF in two different rotors the samples are subjected to the same force The RCF at a number of rotor speeds is provided in Table 1 Maximum allowable runs speed for these rotors must be reduced in any of the following circumstances e When any single cavity load exceeds the rated maximum including the weight of the tube and cap or if the solution density is greater than 1 2 g mL use the applicable square root reduction formula to determine the allowable rotor speed GA 10 speed 6400 rpm
10. 4 white 344497 15 16 x 100 118 5410 blue 870329 30 25 x 105 121 5550 blue 870331 50 29 x 104 123 5640 none Maximum speed when 50 mL glass tubes are run is 1000 rpm GA 10 and GA 24 Rotors Table 3 Acceptable Tube Sizes and Adapters for GA 24 Rotors Tmax at Adapter or RCF at Maximum Cavity Bottom Speed Nominal mm x 9 Tube Nominal Adapter Volume Dimensions Outer Inner Outer Inner Adapter Part mL mm Row Row Row Row Color Number 7 13 x 100 119 104 5460 4770 orange 342642 10 16 x 100 119 104 5460 4770 purple 342641 15 16 x 100 123 108 5460 4950 none Table 4 Beckman Coulter Tubes and Bottles for GA 10 and GA 24 Rotors Tube Dimensions Volume Part mm mL Description Number Adapter Rotor Open Top Tubes 16 x76 10 polyallomer 355640 342641 GA 24 16 x 76 10 polycarbonate 355630 342641 GA 24 16 x 76 10 stainless steel 301108 342641 GA 24 29 x 103 50 polypropylene 357007 none GA 10 29 x 103 50 polycarbonate 357006 none GA 10 Tubes with Snap On Caps 11x 38 1 5 natural polyallomer 357448 344497 GA 10 11x 38 1 5 natural polypropylene 356090 344497 GA 10 11 x 38 1 5 blue polypropylene 356091 344497 GA 10 11x 38 1 5 green polypropylene 356092 344497 GA 10 11x 38 1 5 yellow polypropylene 356093 344497 GA 10 11 x 38 1 5 orange polypropylene 356094 344497 GA 10 11x 38 1 5 natural polypropylene 343169 344497 GA 10
11. GS6 TB 009A AA August 2000 BECKMAN COULTER INSTRUCTIONS FOR USING THE GA 10 AND GA 24 FIXED ANGLE ROTORS In Beckman Coulter GS 6 Series Spinchron Series and GP Series Centrifuges 2000 Beckman Coulter Inc Printed in the USA GA 10 and GA 24 Rotors min Tay gt lt Tmax gt Axis of Rotation GA 10 SPECIFICATIONS Maximum speed 0 0 cee cece eee eee 6400 rpm Critical speed range 00 0 eee eee eee 400 to 1400 rpm Density rating at maximum speed 000 1 2 g mL Relative Centrifugal Field see Table 1 at maximum speed Fmax 123 mm 5640 x g Conditions requiring speed reduction see RUN SPEEDS Number of tube cavities 0 0 eee ee eee 10 Available tubes and bottles 0002000005 see Table 4 Maximum single cavity load at rated speed including tube and cap 120 grams Maximum tube length for full complement of tubes 31 mm diameter 0000000 00e 124 mm Maximum rotor capacity 0 eee eee ee eee 500 mL Approximate acceleration and deceleration times see Table 7 Weight of fully loaded rotor 0006 6 4 kg 14 Ib Rotor material io peda tee Pie Aa Cake ae Re aluminum The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing th
12. ND LOADING For runs at other than room temperature refrigerate or warm the rotor beforehand for faster equilibration A N CAUTION The centrifuge drive shaft can be bent or broken if the rotor is forced sideways or dropped onto it Install the rotor by centering it over the shaft and carefully lowering it straight down 1 The rotor is marked with four dots at 3 6 9 and 12 o clock positions indicating the direction of the grooves Align the dots in the rotor top with the centrifuge drive pins Install the rotor into the centrifuge by centering it over the drive shaft and lowering it straight down Make sure that the grooves in the rotor drive cavity are seated on the centri fuge drive pins if the rotor is not correctly seated the lid can not be secured to the rotor gt NOTE Do not use adapters in which glass tubes have previ ously broken pieces of glass will be embedded in the rubber and will damage or break subsequently used containers 2 Load the filled containers symmetrically into the rotor If fewer than the maximum number of tubes or bottles or multi tube adapters are being run they must be arranged symmetrically in the rotor Opposing containers must be filled to the same level with liquid of the same density 3 Put the lid in place and tighten by hand as firmly as possible 4 When the rotor is seated ensure that the rotor tie down nut O ring is in place and in good condition then place the tie down nut on th
13. Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 SPAIN Beckman Coulter Espa a S A Avda del Llano Castellano 15 28034 Madrid Spain Telephone 91 728 7900 Fax 91 728 7971 SWEDEN Beckman Coulter AB Archimedesvaegen 2 Box 11156 168 11 Bromma Sweden Telephone 08 98 53 20 Telefax 08 98 74 34 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 0800 850 810 Fax 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Bucks HP11 1JU England U K Telephone 01494 441181 Fax 01494 447558 e mail beckman_uk beckman com Authorized dealers in other countries 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 Sales 1 800 742 2345 Service 1 800 551 1150 Internet www beckmancoulter com Telex 678413 Fax 1 800 643 4366 fry QO Printed on recycled paper 2000 Beckman Coulter Inc amp LS EN ISO 9001
14. d or modified 4 Thermoplastic rotors or components used in some bench top centrifuges are warranted for one 1 year from date of purchase DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR RE PLACEMENT OF THE PRODUCT Beckman Coulter Worldwide Bioresearch Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email myork beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 905 819 1234 Fax 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100022 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 EASTERN EUROPE MIDDLE EAST AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 994 07 07 Fax 41 22 994 07 00 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359
15. e centrifuge drive shaft Tighten it firmly by inserting the torquing bar 356036 through the cross drilled hole in the tie down nut and turning the nut clockwise until it is tight If the rotor is left in the centrifuge between runs tighten the tie down nut before each run GA 10 and GA 24 Rotors AN WARNING Do not substitute a metal object for the plastic tie down nut supplied with this rotor If a tie down nut comes off the shaft during centrifugation the plastic nut furnished will break apart in the chamber causing minimal damage to the instru ment A loose metal object could substantially damage the rotor chamber and lid and could potentially escape the chamber into the labora tory causing personal injury or property damage AN CAUTION Failure to securely tighten the tie down nut may allow the tie down nut and the lid assembly to come off This could cause severe damage to the rotor and the centrifuge OPERATION Refer to the instrument instruction manual for centrifuge operation Table 5 indicates approximate acceleration and deceleration times for these rotors in both 60 Hz and 50 Hz instruments To avoid sample mixing caused by rapid deceleration always run GA 10 and GA 24 rotors with the centrifuge set for LOW brake gt NOTE For low temperature runs precool the centrifuge and rotor without samples at 0 C for 30 minutes at 2000 rpm N CAUTION Make sure the tie down nut is tight before each run GA 1
16. fe g Force Safe Speed Tube Description mL mm RCF RPM B D SST RB SB 10 16 x 100 B D Vac RB plain 10 16 x 100 Kimax 45049 RB 10 16 x 100 Kimax 45200 LT G SC 10 17x 116 Pyrex 9820 RB 10 16 x 100 Pyrex 8140 LT G 12 17x 117 B D SST RB SB 15 16 x 130 B D Vac RB plain 15 16 x 130 Corex 8060A LT 15 17 x 120 Corex 8080A LT G 15 17 x 120 Corex 8442 RB 15 16x 115 Corvac RB SB 15 16 x 131 Kimax LT G 15 17x 118 Pyrex 8500 ST 15 17x 110 Pyrex 8080 LT G 15 17 x 120 Pyrex 8320 ST 40 29x 116 2500 4200 Kimax 45212 ST 50 29x 118 1000 2600 Pyrex 8280 ST 50 29x 115 2500 4200 Most common usage Speed must be reduced to 1000 rpm when some 40 and 50 mL glass tubes are used in the GA 10 rotor because no adapters are used Code B D Becton Dickinson G graduated SB silicone gel barrier Vac Vacutainer B D Dispo disposable The following are registered trademarks Corvac Sherwood Medical Industries Inc Pyrex and Corex Corning Glass Works Kimax Kimble Division of Owens Illinois SST and Vacutainer Becton Dickinson and Company LT long taper SC screw capped FB RB ST flat bottom round bottom short taper Table 7 Recommended g Force Limits to Minimize Breakage of Certain Glass Tubes and Bottles Made by Other Manufacturers in the GA 24 Rotor Volume Dimensions Safe g Force Safe Speed Tube Descri
17. gt NOTE To obtain copies of referenced publications Contact Beckman Coulter Inc Technical Publica tions Department 1050 Page Mill Road Palo Alto CA 94304 U S A phone 650 859 1753 fax 650 859 1375 Contact Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed on the back cover of this manual for detailed information on ordering parts and supplies For your convenience a partial list is given below GA 10 GA 24 REPLACEMENT ROTOR PARTS Rotor assembly 1 0 0 0 cee EENI eee 349826 ereenn eke sg 349827 Tie down nut 0 a ccc eee nes 356035 0 0 cee 356035 O ring Buna N rubber 0 0 cece eee eee eee OD TIT sence ee es eet 927571 Lidiassembly a eea E e a ee a eee esas 355816 0000s 355816 O ring Buna N rubber 0 0 e cece eee eee 012780 04 012780 SUPPLIES Adapters soin sande San a Raid MEG eae eRe se Table 2 5 os 36 as see Table 3 Tubes and bottles 0 0 0 cece nee see Table 4 see Table 4 Torquing bar jo gis ogee ie SAN ea SB aan 356036 05 oes ieee RS 356036 Rotor Cleaning Kit 0 0 00 0 cee eee eee eee 339558 eo teks Gees Ga 339558 Beckman Solution 555 1 qt 0 0 e eee eee eee 339999 eas aa Sah aes 339555 Silicone vacuum grease 1 0Z 0 0 0 cee eee eee eee 335148 005 335148 Anti Seize 1 1 2 02 0c eee nes 961660 961660 BENCHTOP ROTOR WARRANTY
18. lled tubes may be used to purify a large quantity of virus in a step gradient They are used for binding studies and to separate serum from whole blood The rotor body and lid are made of aluminum and are anodized for corrosion resistance the rotor body is black and the lid is blue The rotor lid is secured to the rotor by air pressure differential caused by the rotor as it spins Grooves inside the rotor drive hole mate with drive pins on the centrifuge drive shaft to prevent the rotor from slipping during acceleration and deceleration See the Warranty at the back of this manual for warranty information TUBES AND BOTTLES The GA 10 can accommodate up to ten 50 mL tubes Acceptable tube sizes and the adapters required to hold them in the cavities are listed in Table 2 Beckman Coulter tubes available for the use with the GA 10 rotor are described in Table 4 The GA 24 can accommodate up to twenty four 15 mL tubes in two rows of cavities an outer row of sixteen and an inner row of eight Acceptable tube sizes and the adapters required to hold them in the cavities are listed in Table 3 Beckman Coulter tubes available for use with the GA 24 rotor are described in Table 4 Table 2 Acceptable Tube Sizes and Adapters for GA 10 Rotors Nominal Nominal Imax at Adapter RCF at Adapter Tube Volume Dimensions or Cavity Bottom Maximum Speed Adapter Part mL mm mm x 9 Color Number 1 5 11 x 38 58 2660 white 344497 1 8 11x39 57 261
19. o prevent buildup of residues 1 2 Remove the O rings before washing Wash the rotor and lid in a mild detergent such as Beckman Solution 555 339555 that won t damage the rotor The Rotor Cleaning Kit 339558 contains two brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water Rinse the cleaned rotor and components with distilled water Air dry the rotor and lid upside down Do not use acetone to dry the rotor GA 10 and GA 24 Rotors Before reinstalling the rotor lightly lubricate the drive hole of the rotor with Anti Seize to prevent the rotor from sticking as follows 1 Dip a swab into the lubricant 2 Draw the coated swab through a paper towel to remove excess lubricant 3 Lightly coat the inside of the drive hole with the lubricant remaining on the swab DECONTAMINATION If the rotor and or accessories becomes contaminated with radioactive material it should be decontaminated using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash 3 gt NOTE IsoClean can cause fading of colored anodized surfaces Use it only when necessary and remove it promptly from surfaces While Beckman Coulter has tested these methods and found that they do not damage components no gua
20. ption mL mm RCF RPM Pyrex Kimax B D or 3 10x 75 Dispo RB or FB or less Pyrex LT 3 11x75 B D Vac RB plain 5 12x75 Kimax RB 5 12x75 Pyrex 9820 RB 5 12x75 B D SST RB SB 7 12 x 100 max 6400 B D Vac RB plain 7 13 x 100 max 6400 B D SST RB SB 10 16 x 100 3000 4650 B D Vac RB plain 10 16 x 100 max 6400 Kimax 45059 RB 10 16 x 100 max 6400 Kimax 45200 LT G SC 10 17x 116 900 2600 Pyrex RB 10 16 x 100 max 6400 Pyrex 8140 LT G 12 17 x 117 900 2600 B D SST RB SB 15 16 x 130 900 2600 B D Vac RB plain 15 16 x 130 900 2600 Corex 8060A LT 15 17 x 120 900 2600 Corex 8080A LT G 15 17 x 120 900 2600 Corex 8442 RB 15 16 x115 max 6400 Corvac RB SB 15 16 x 131 max 6400 Kimax LT G 15 17 x 118 900 2600 Pyrex 8500 ST 15 17x 110 900 2600 Pyrex 8080 LT G 15 17 x 120 900 2600 Most common usage Code B D Becton Dickinson Dispo disposable FB flat bottom G graduated LT long taper RB round bottom SB silicone gel barrier SC screw capped ST short taper Vac Vacutainer B D The following are registered trademarks Corvac Sherwood Medical Industries Inc Pyrex and Corex Corning Glass Works Kimax Kimble Division of Owens Illinois SST and Vacutainer Becton Dickinson and Company GA 10 and GA 24 Rotors GA 10 and GA 24 Rotors 14 CARE AND MAINTENANCE MAINTENANCE gt NOTE Do not use sharp tools on the rotor as the surface can be scratched Cor
21. rantee of decontamination is expressed or implied Consult your laboratory safety officer regarding the proper decontamination methods to use If the rotor or accessories are contaminated with toxic or pathogenic solutions follow appropriate decontamination procedures Check the chemical resistances list in Chemical Resistances to be sure the decontamination method will not damage any part of the rotor A registered trademark of Permatex 2 In the United States contact Nuclear Associates New York in eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo 3 In the United States contact Biodex Medical Systems Shirley New York internationally contact the U S office to find the dealer closest to you 15 GA 10 and GA 24 Rotors STERILIZATION AND DISINFECTION e The rotor and all rotor components can be autoclaved at 121 C for up to an hour Remove the lid from the rotor and place the rotor and lid in the autoclave upside down Ethanol 70 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anodized surfaces Use the minimum immersion time for each solution per laboratory standards While Beckman Coulter has tested these methods and found that they do not damage the rotor or components
22. rosion begins in scratches and may open fissures in the metal with increased use Periodically at least monthly inspect the rotor especially inside cavities for rough spots or pitting white powder deposits frequently aluminum oxide or heavy discolor ation If any of these signs are evident do not run the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center Regularly check the condition of the O ring 927571 in the tie down nut 356035 if it is worn or damaged replace it Before using the tie down nut check it for damage such as distortion or splitting replace it if it is damaged If no damage is apparent lightly coat the O ring in the base of the nut with silicone vacuum grease 355148 Do not use any type of lubricant or grease on the threads of the tie down nut or the threads of the drive shaft Store the rotor in a dry environment not in the centrifuge with the lid removed Refer to Chemical Resistances publication IN 175 for the chemical compatibilities of rotor and accessory materials Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center CLEANING Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently t
23. rough the critical speed range is characterized by some vibration Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed rw to the standard acceleration of gravity g according to the following formula RCE 12 g where r is the radius in millimeters w is the angular velocity in radians per second 2 RPM 60 and g is the standard acceleration of gravity 9807 mm s2 After substitution RCF 1 127 REM 1000 The GA 10 rotor should always be run with the LOW brake setting to prevent sample mixing GA 10 and GA 24 Rotors GA 24 SPECIFICATIONS Maximum speed 0 0 c ee eee eee nee nee 6400 rpm Critical speed range 0 2c eee ee ee ee 400 to 1400 rpm Density rating at maximum speed 006 1 2 g mL Relative Centrifugal Field at maximum speed see Table 1 outer row Fmax 123 mM 6 eee 5640 x g inner row Fmax 108 mmM 2 ee eee 4950 x g Conditions requiring speed reduction see RUN SPEEDS Number of tube cavities spase erota Einn eee eee 24 Available tubes and bottles 00000 see Table 4 Maximum single cavity load at rated speed including tube and cap 36 grams Maximum tube length for full complement of tubes 19 mm diameter 000000000 eee 124 mm Maximum rotor capacity 0 eee ee eee ee 360 mL Approximate acceleration and deceleration times
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