Home

System Manual

1. Main power switch Figure 7 3 Imaging chamber external gas ports GAS IN means the direction of flow is into that port Similarly the port labeled GAS OUT means that flow can be exhausted out of this port WARNING Use only isoflurane with the IVIS Lumina XR DO NOT USE FLAMMABLE ANESTHESIA GAS 30 IVIS Lumina XR Imaging System Manual CAUTION Caliper recommends using the XGI 8 Gas Anesthesia System when imaging small animals Figure 7 4 This system supplies a controlled amount of isoflurane to the imaging chamber and continuously reduces the build up of isoflurane in the chamber If you want to use a gas other than the recommended isoflurane oxygen gas mixture or pure air contact Caliper technical support Figure 7 4 XGI 8 Gas Anesthesia Delivery System NOTE Be careful to use only tubing and other plumbing fixtures that do not fluoresce or phosphoresce glow in the imaging chamber Contact Caliper technical support for a list of acceptable materials 7 4 Door Operation The IVIS Lumina XR imaging chamber door has custom designed hinges seals and a four point closure mechanism The door is designed to provide a light tight seal over numerous opening closing cycles and should close easily without excessive handle turning resistance The door has shielding to prevent the escape of X ray radiation and should not be tampered with or modified in any way The door also contains part of the X ray safety in
2. The only exception to this is removing the light source module front panel mounted lamp assembly for tungsten halogen lamp replacement see page 53 Do not modify the IVIS Lumina XR in ANY manner by making any kind of hole or aperture in the instrument or removing any component that is part of the radiation shielding Optional optical emission filters are available for the VIS Lumina XR see Optical Filter Wheel page 25 and Appendix C page 63 When installing the optical filter wheel make sure that the steel Illumination Plate Assembly has been re installed since this plate is also part of the X ray shielding 12 IVIS Lumina XR Imaging System Manual This page intentionally blank Dr Y Ca ec 4 Legal Notices Limited IST soora A A ee Hs 13 Pais p ai par aaa AAA AAA A A 14 Trademarks gt e e m ae e a a A A A oe i Bow ee on me G 14 4 1 Limited Warranty Caliper Life Sciences Inc Caliper provides the following limited warranty for each new IVIS Lumina XR System as follows Limited Warranty 111 1 a il a This Limited Warranty for the System extends to the original purchaser Customer for a period of one 1 year following installation of the System and is not assignable or transferable to any successor During the Limited Warranty Caliper will repair or replace at Caliper s sole option any defective parts 1f such repair or replacement is needed because of System malfuncti
3. Install the equipment in an environment where e The temperature does not fluctuate widely and is maintained between 15 25 C 59 77 F e The humidity does not exceed 80 e No strong electric or magnetic fields exist 6 IVIS Lumina XR Imaging System Manual Heat e No vibrations are present e No corrosive gases are present e High amounts of dust are not present e No open flame is present e There is sufficient space behind the VIS Lumina XR equipment A minimum space of four inches from the flat surface of the rear panel should be provided behind the IVIS Lumina XR to provide unobstructed air flow and access to the main power on off switch e The work space is level The system should be situated away from heat sources such as open flames radiators heat registers stoves and other heat generating electrical equipment Water amp Moisture VOLTAGE Do not use this product near water for example near a sink or wet room due to risk of electric shock electrical damage and or equipment failure 2 6 Cleaning or Moving the System Components Cleaning Liquid Entry VOLTAGE Do not use liquid or aerosol cleaners and never spill liquid of any kind on any of the IVIS Lumina XR components Sprays and liquids that come into contact with the IVIS Lumina XR hardware may result in damage to the system or electrocution For more details on proper care of the system see Cleaning the IVIS Lumina XR page 34
4. System Manual IVIS Lumina XR Real Time Bioluminescence Fluorescence amp X Ray Imaging System October 2009 2009 Caliper Life Sciences Inc All rights reserved PN 125644_Rev00 To order replacement parts contact Sales Order Management Caliper Life Sciences 68 Elm Street Hopkinton MA 01748 USA Tel 1 508 4971 3302 Fax 1 508 435 0967 E mail order entry caliperLS com For technical support contact Caliper Life Sciences 68 Elm Street Hopkinton MA 01748 USA Tel 1 877 522 2447 US Tel 1 508 435 9500 Fax 1 508 435 3439 E mail tech support caliperLS com www caliperLS com IVIS and Living Image are registered trademarks or trademarks of Caliper Life Sciences Inc The names of companies and products mentioned herein may be the trademarks of their respective owners Apple Macintosh and QuickTime are registered trademarks of Apple Computer Inc Microsoft PowerPoint and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries Adobe and Illustrator are either registered trademarks or trademarks of Adobe Systems Incorporated in the United States and or other countries Intel is a registered trademark of Intel Corporation DIr Y Ca cc Contents 1 Welcome asa a a ARES AAA AAA 1 1 1 Introduction os ea eee ee irene de he eee 1 1 2 Caliper Technical Support so saosaoa 2 2 Important Safety Instructions e
5. The fluorescence light source module Figure 10 3 provides the fluorescence excitation light This light source consists of a 150 watt quartz tungsten halogen lamp with a dichroic reflector Figure 10 4 shows the relative spectral radiance output of the lamp reflector combination and indicates emission throughout the VIS Lumina XR wavelength range of 400 950 nm 46 IVIS Lumina XR Imaging System Manual Lamp assembly RS 232 connector for control cable Manual intensity control must remain in R position to function correctly Lamp indicator light Fiber optic cable On off power switch Fan Figure 10 3 Fluorescence light source module front view left and rear view right 1 0 0 8 0 6 0 4 0 2 Relative Spectral Radiance 0 0 400 500 600 700 800 90 Wavelength nm Figure 10 4 Relative spectral radiance for the quartz halogen lamp The dichroic reflector reduces infrared transmission gt 700 nm enough to prevent overheating of fiber optic bundles while still allowing sufficient infrared light thoughput to permit imaging at these wavelengths The lamp intensity level is computer controlled by the Living Image software The user can adjust the lamp output intensity by means of software to settings of low or high The fluorescence light source module operates under software control therefore manual adjustment of the front panel lamp potentiometer is disabled The lamp output is delivered to the excitation
6. Moving the IVIS Lumina XR You can move the light source module on the laboratory bench within the extent of the fiber optic cable Be careful not to bend the fiber optic cable minimum radius of bending 3 inches 7 6 cm CAUTION The IVIS Lumina XR is sensitive scientific equipment and should not be moved by any user unless the system is located on the XWS 260 workstation Due to the risk of potential damage it is critical that only a trained Caliper technician moves the IVIS Lumina XR If it is necessary to relocate the IVIS Lumina XR contact Caliper technical support For more details on moving the IVIS Lumina XR on the XWS 260 workstation see page 57 A Y Caliper Chapter 2 Important Safety Instructions 7 2 7 Power Considerations Power Sources The IVIS Lumina XR is configured for the voltage requirements of the installation locality that was specified at the time of order If the IVIS Lumina XR is moved to another area make sure that the same voltage requirements exist VOLTAGE The IVIS Lumina XR can operate at multiple voltages 100 240 VAC however you are not permitted to change the input voltage to any of the system components Several internal modifications are required for voltage change If the operating voltage must be changed contact Caliper technical support Power Cord Protection Power supply cords should be routed so that they are unlikely to be walked on or pinched by items placed upon or against the
7. Weight 22 lbs 10 Kg Computer Monitor Specifications Computer Monitor Description Flat screen Power requirements 0 6 Aat 120 V 0 35 A at 240 V 50 60 Hz Dimensions with stand 17 5 x 17 5 x 9 45 cm x 45 cm x 23 cm Weight with stand 33 lbs 15 Kg Dimensions without stand 17 5 x 17 5 x 2 5 45 cm x37 cm x 6 5 cm Weight without stand 20 Ibs 9 Kg 6 10 Environmental Requirements Temperature 15 Cto 25 50 F to 78 F Humidity 0 to 80 non condensing Type of use Indoor Imaging chamber shelf temperature Ambient to 37 C Altitude rating lt 2000 meters 6560 ft Pollution degree 2 Installation category II Y Caliper ifeSciences 7 Operating the IVIS Lumina XR Starting the Lumina XR Imaging System 2 a a a 27 Restarting the System After a Power Outage a a 00 29 Gas PIDA bss ae B eh oh Se AMR Gh ve Ah A eee Bd Bok Boh a wh we 29 Door Opera saca eo acer d Ss Amt es we Ss Gk So Go See Se a Sea Sak 30 Magina Basics o a od ho 6 Ha 84 a w oe We AAA WSR Be BO acer 31 System Shut Down Procedure e 32 The IVIS Lumina XR is intended for use in biophotonic imaging procedures The system 1s designed to detect extremely low level light emissions that are orders of magnitude dimmer than can be seen with the naked eye The IVIS Lumina XR allows you to monitor and record cellular and genetic activity within a living organism in real time The imag
8. anesthesia gas plumbing 29 anesthesia delivery system 30 B bandgap 48 bandpass filter 48 black paper 31 burn hazard 10 C Caliper technical support 2 camera noise 41 CCD camera 20 centering a subject 31 chemical amp biological safety 11 cleaning imaging system 6 cleaning solutions 34 cleaning the system 34 35 compliance 17 computer 25 specifications 26 computer monitor specifications 26 cover 11 D door operation 30 E electrical power 44 electrical safety 9 emission bandpass 49 emission filter transmission curve 48 emission filter wheel 47 emission filter wheel replacement 63 65 emission fluorophore spectra 48 environmental considerations heat 6 water and moisture 6 environmental requirements 26 excitation filter 49 filter transmission curve 48 filter wheel 46 47 fluorophore spectra 48 eye and burn hazard 10 F filter lock 50 filter replacement 55 filter spectra 48 fluorescence equipment See Fluorescence Module Fluorescence Module installation requirements 43 system components 45 fluorescent dyes 49 fluorescent imaging 50 51 fluorescent proteins 49 fuse 44 55 fuse replacement 52 53 G gas plumbing 29 glowing materials 32 H hardware problems 51 hazardous voltage symbol 4 hazards 10 heat safety 6 humidity 44 l imaging chamber 23 24 door 30 gas plumbing 29 specifications 24 stage curtain 31 imaging system cleaning 6 servicing 7 installation requirements 43 isoflurane gas anest
9. e Low dark current e Thermoelectrically cooled e 16 bit CCD digitization e Low noise electronic readout for extremely low background images CCD Camera Specifications CCD Camera Specification Sensor Type Back illuminated CCD Format 1024 x 1024 Pixel Dimensions 13x 13 um Quantum Efficiency gt 85 400 700nm gt 50 350 900nm 550 650nm Readout Noise bin 1 lt 2 e RMS Dark Charge lt 0 0015 e pixel sec 6 2 X Ray System amp Components The X ray modality of the VIS Lumina XR operates as a separate capability within the Living Image operating environment The instrument can operate in the following modes e Bioluminescence mode e Fluorescence mode e White light photographic A Y Caliper Chapter 6 IVIS Lumina XR Components amp Specifications 21 e X ray mode e Any combination of bioluminescence fluorescence and or X ray mode Operating in X ray mode requires activation with a key operated selector switch on the imaging chamber front panel For more details see page page 28 The X ray source is located underneath the imaging chamber in a separate shielded enclosure in the electronics drawer The X ray beam cone is directed upward through a transparent radiolucent plate in the imaging chamber floor The beam passes through the movable shelf through the subject animal and finally impinges on the scintillation plate The CCD camera captures the scintillation image produced in the plate Wh
10. s w 2 2 e e a E E N A R e a e 17 6 IVIS Lumina XR Components Specifications 19 6 1 CCD Camera 3 224 24a te dadas daa ELAS ERED AES EOE BE AS 20 6 2 X Ray System amp Components 20 6 3 X Ray Scintillation Module 0000000002 2 22 6 4 X Ray System Control Panel 2 2 0 02 2 a 22 6 5 Key Selector Switch amp Lost Keys 2 2 0 02 ee 23 ii IVIS Lumina XR Imaging System Manual 6 6 Imaging Chamber s s a a 24 44 6 eR A ae ee ae eR ee ee ee eS 6 8 Optical Filter Wheelc can see aa ee ea ma eae Bae ee ee eae Paes 6 9 Acquisition Computer aoaaa a 6 10 Environmental Requirements ooa a a a 7 Operating the IVIS Lumina XR 0 000000 204s 7 1 Starting the Lumina XR Imaging System aoaaa 7 2 Restarting the System After a Power Outage aaao aa e 7 3 Gas Plumbing oca e esca oc er eaea h ds a e ed T A Door Operation s ss s w daa ag ab a eee a e A a 0 Imaging Basics scs os as ew a e be ek eS a ee a ea a 7 6 System Shut Down Procedure a a 8 Care Maintenance o o 8 1 Surveying the VIS Lumina XR for Radiation Leakage 8 2 Maintenance amp Safety Checks 2 e 8 3 Cleaning the VIS Lumina XR 2 2 0 000 0000 0000200007 8 4 Cleaning the Scintillation Plate Window amp Holder o a 9 Troubleshooting 82 ee eS eER OD RRER ES RED ER ORT Se RRR a 9 1
11. U S A excluding its conflicts of laws principles and excluding the United Nations Convention on Contracts for the International Sale of Goods a Vil The detection and imaging of light originating within mammals is the subject of several issued patents and pending patent applications in the United States and around the world including U S Patent Numbers 5 650 135 6 217 847 6 649 143 6 890 515 and European Patent Commission Number EP0861093 for which Caliper is the exclusive licensor The use of an IVIS Imaging System for such applications requires a sublicense from Caliper In addition many of the hardware and software components of the System are the subject of various issued patents and pending patent applications owned by Caliper including United States Patent Number 6 614 452 Graphical User Interface for In Vivo Imaging 6 775 567 Improved Imaging Apparatus 7 113 217 Multi view Imaging Systems 7 190 991 Method and Apparatus for 3 D Reconstruction of Light Emitting Sources 7 403 812 Method and Apparatus for Determining Target Depth Brightness and Size Within a Body Region 6 894 289 Fluorescence illumination assembly for an imaging apparatus and 6 919 919 Light calibration device for use in low level light imaging systems 4 3 Trademarks IVIS and Living Image are registered trademarks or trademarks of Caliper Life Sciences Inc The names of companies and products mentioned herein may be the trademarks of
12. WARNING If you need to replace the tungsten halogen lamp or lamp assembly in the light source module wait until the lamp and socket are cool Confirm that the components are cool before proceeding See Lamp Replacement page 53 3 4 Mechanical Safety The imaging chamber of the IVIS Lumina XR is heavy and weighs 160 lbs 73 kg IMPORTANT ALERT The IVIS Lumina XR may only be moved on the XWS 260 workstation For more details see Appendix A page 57 The IVIS Lumina XR has many internal motorized components that can move at any time The imaging stage can move when the door is open Care should be taken to keep hands and equipment away from the sides of the platform when it is moving Never place anything underneath the platform Do not attempt to put anything into the lens opening of the camera as there are optical components that can be compromised or damaged If the imaging chamber makes an unusual noise or appears to be jammed turn off the power switch located on the back of the chamber The X ray scintillation plate is a delicate part and should not be handled with bare hands or sprayed with cleaning agents WARNING If the gas hoses become caught kinked or disconnected do not operate the instrument Over exposure to anesthesia gas may occur CAUTION DO NOT touch or expose the four diffusing reflectors and the exposed emission filter to contaminants described in System Components page 45 and shown in Figur
13. all servicing to Caliper technical support 16 IVIS Lumina XR Imaging System Manual WARNING Do not for any reason attempt to defeat the built in safety interlocks described in Safety Features amp Safety Systems page 16 Operating the IVIS Lumina XR without the safety interlocks can result in exposure to X rays Exposure to X rays can cause serious bodily injury or death Refer all servicing to Caliper technical support 5 3 X Ray Dose Limits A sample of Caliper Model IVIS Lumina XR has been tested at maximum operating conditions Caliper has determined the local X ray dose rate at a distance of 5 cm from the surface of the equipment is less than 1 0 mSv h Caliper declares that the Product IVIS Lumina XR system conforms to 1 1996 29 Euratom Directive Dose rate of 1 mSv h at 10 cm from any accessible surface under normal operating conditions US CFR21 Part 1020 40 Regulation Dose rate of 0 5mrem h at 5cm outside of the external surface under maximum operating conditions in accordance with the following standard IEC 61010 1 2001 Standard Dose limit of 0 Smrem h at 5 cm from the surface of the equipment under maximum operating conditions N Caliper certifies that Lumina XR system has achieved the objectives of 1 ICRP 60 recommendations of annual public dose limit of 100 mrem 2 ICRP 103 recommendations of annual public dose limit of 100mrem 3 US OSHA workplace annual public dose limits of 100mrem and other inte
14. country where the equipment is operated This includes all local state and federal regulations In some states of the US it may be necessary to register radiation sources with the governing state and or local public health agencies before operating the instrument Equipment registration may be required immediately or within 30 days of acquiring the equipment Owners and operators of the IVIS Lumina XR are responsible for contacting the appropriate public health agencies for registration information that pertains to installation of the IVIS Lumina XR If you need assistance with this requirement contact Caliper technical support For more details and contact information see Safe Operating and Emergency Procedures for the Operation of the IVIS Lumina XR Cabinet X Ray System This document was provided with the pre installation instructions WARNING A Caliper employee will conduct a radiation leakage survey and safety tests when the IVIS Lumina XR is installed Caliper employees are trained in radiation safety However check with your local radiation control authority to determine the specific radiation survey requirements at your facility If necessary have a qualified expert other than a Caliper employee survey the installation before operating the instrument 2 5 Environmental Considerations for the System Components Locating the IVIS Lumina XR Before the VIS Lumina XR is installed consider the proper environment for the components
15. includes many cables and lines To avoid damaging the system components it is very important to closely follow all directions Figure A 1 I VIS Lumina XR configured with the XWS 260 Workstation A 1 Shutting Down the Imaging System 1 Close the Living Image software and save any information of interest at the prompt g g y promp 58 IVIS Lumina XR Imaging System Manual 2 Return the X ray ON key selector switch to the OFF position and remove the key 3 Turn off the VIS Lumina XR imaging chamber 4 Turn off the computer using the standard Windows shut down procedure 5 Turn off the power to the other system components and power surge protection devices 6 If moving the system unplug the devices from the wall If you have any problems during the shut down procedure please contact Caliper technical support for assistance NOTE The Emergency OFF switch is not intended as a main X ray source control and should not be used to turn the X ray function ON or OFF on a routine basis It should only be used in the unlikely situation where the X ray source must be immediately turned OFF Under normal circumstances it should be left in the ON position and left as is A 2 Moving the Imaging System on the XWS 260 Workstation 1 Unlock the wheels on the XWS 260 workstation and carefully roll the workstation to the new location CAUTION When moving the imaging system on the XWS 260 workstation be sure to grasp and push
16. of View C y System Status X Rays will be produced X Rays will be produced rdle when energized z Demand Measured when energized Service 10 cm Acquire Service fio cm Camera Temp pm g BRE Acquire Sequence Setup Se Stage Temp 37 0 quence Setup S foco Jem 00 p Initialize Focus fuse subject height y e Locked Subject height fo 00 3 cm Focus use subject height Temperature A Click the temperature square to display the demand and measured temperatures of the camera and imaging chamber Figure 9 1 VIS System Control panel in the Living Image software Problem Possible Cause Corrective Action Measured temperatureis Ambient temperature may be too Verify that the room temperature is within operational warmer than the demand high camera air vents my be limits Check air vents in the camera head by removing temperature blocked or system needs service protective cover and inspecting Contact Caliper A problem may exist with the technical support for assistance camera Something is obstructing 1 Open the door to the imaging chamber and visually the stage inspect the stage 2 Remove anything that is physically obstructing the stage If there is no obstruction and or the stage still does not move contact Caliper technical support for assistance 40 IVIS Lumina XR Imaging System Manual 9 2 Photographic Image Is Unacceptable Photog
17. on the sample stage WARNING DO NOT disconnect or reattach the electrical control cable that connects the fluorescence equipment excitation filter assembly to the light source module and IVIS Lumina XR electronics tray when the power is on See System Components page 45 Figure 10 1 and Figure 10 2 for photographs of these components Disconnecting or reconnecting the control cable when the system has electrical power will damage the system Always turn off the front panel light source module switch and the rear mounted ON OFF switch on the IVIS Lumina XR before making or breaking any of these cable connections Fuse Replacement WARNING The following procedure can expose the user to hazardous voltages unless the electrical power to the fluorescence light source module is completely eliminated As instructed in the procedure turn off the lamp from the module front panel ON OFF switch and remove the electrical power to the module by disconnecting the plug from the surge protector and the back of the module 1 N See the Fuse Replacement section in the separate manual for the light source module SCHOTT Fostec DCR III Direct Current Regulated Light Source User s Manual and Technical Reference Press the ON 1 OFF O switch to the OFF 0 position on the light source module 3 Remove AC line cord from the AC power receptacle on the surge protector 4 Remove the AC line cord from the power entry module on the rear pan
18. sets Instructions for mounting are included with the set Contact Caliper technical support for information on installing additional optical filters or visit us at https store caliperls com A 12 position excitation filter wheel with 10 equally spaced narrow band filters is attached to the back of the imaging chamber For a details on filter wheel options see Table 10 1 page 49and Appendix B page 61 The filter wheel settings are selected in the Living Image software For more details see the Living Image Software User s Manual 6 9 Acquisition Computer The computer contains an Intel family processor and Windows operating system Figure 6 1 Microsoft Office is installed as well as the Living Image software that controls the IVIS Lumina XR The computer controls the VIS Lumina XR hardware including the CCD camera A printer can be connected to the computer Computer Features e High speed Windows based PC e Microsoft Windows family operating system e Living Image software installed This software controls the VIS Lumina XR and displays and analyzes image data e CD burner installed for data storage and transport e Network ready e 20 high resolution flat screen monitor for image viewing e Microsoft Office installed 26 IVIS Lumina XR Imaging System Manual Computer Specifications Power requirements 1 0A at 120 V 0 5 A at 240 V 50 60 Hz Dimensions 15 75 x17 x 4 75 40 cm x 44 cm x 12 cm
19. solutions in the imaging chamber Gently wipe the imaging chamber surfaces as described on page 35 Table 8 1 Acceptable cleaning solutions for the IVIS Lumina XR imaging chamber Cleaning Solution Manufacturer Cidexplus Solution 3 4 glutaraldehyde Johnson amp Johnson Medical 70 methyl alcohol 30 deionized water solution Y Caliper 4 Chapter 8 Care amp Maintenance 35 ifeSciences Table 8 1 Acceptable cleaning solutions for the IVIS Lumina XR imaging chamber continued Cleaning Solution Manufacturer 70 ethyl alcohol 30 deionized water solution Sporicidin Sterilizing Solution 1 56 phenol Sporicidin International Clidox s Disinfectant Pharmacal Research Laboratories Inc NOTE Caliper makes no claims as to the sterility of the IVIS Lumina XR imaging chamber after using the solutions in Table 8 1 Please refer to the manufacturer s literature for information as to the applicability of the compound for the organism of interest It is recommended that you use a lint free wipe such as Scott Pure wipe or a Kaydry EX L wipe to minimize the presence of particulate matter in the imaging chamber After saturating a lint free wipe clean the internal surfaces using a gentle circular motion Use extra care when cleaning the radiolucent insert since it is a delicate assembly Do not pour or spray the solution directly onto internal surfaces especially the garage housing the scintillation se
20. source module by turning the retaining screw counter clockwise using a straight blade screwdriver The retaining screw will be disengaged from the front panel but will remain in place in the lamp assembly 54 IVIS Lumina XR Imaging System Manual 6 Remove the lamp assembly from the light source module by slowly pulling outward 7 Check the lamp assembly to verify that the lamp and socket are cool before proceeding 8 Push back on the lamp holder release lever to raise the lamp L Figure 10 12 9 Gently unplug the ceramic lamp socket K E DD EKE DOL Figure 10 12 Light source module 10 Lift the release lever Insert a new lamp into the lamp holder Push the lamp all the way down Be careful not to touch the bulb or the inside of the reflector Fingerprints may affect the light output 11 Push the ceramic socket onto the two lamp pins until flush with the lamp base 12 Return the Modulamp assembly into the recess and tighten the captive thumbscrew 13 For complete Modulamp assembly removal and installation see sections 5 1 and 5 2 in the SCHOTT Fostec DCR III Direct Current Regulated Light Source User s Manual and Technical Reference 14 Reattach the AC line cord to the back of the module and then to the AC receptacle on the surge protector 15 Return the ON OFF switch to the ON 1 position the red indicator will be visible on the switch NOTE For the software to control lamp
21. surface Figure D 3 Remove the flathead screws A Y Caliper Chapter D Removing amp Replacing the Scintillation Plate Holder 69 D 2 Replacing the Scintillation Plate Holder 1 To re install the plate holder orient the scintillation plate mounting disk so that the un chamfered hole is towards the rear of the imaging chamber and slide the paddle underneath this disk Figure D 4 Figure D 4 Orientation of the paddle mounting 2 Reattach the plate holder using the 3 flathead screws that were previously removed Figure D 3 3 Rotate holder clockwise until it is horizontal with the back of the imaging chamber Use a gentle pushing motion to avoid bending the paddle up or down 4 Re install the sheet metal cover with the two 6 buttonhead screws 5 Carefully grip the edge of the paddle when it is under the cover and pull it back to a position about 45 degrees from the center of the imaging stage Figure D 5 Figure D 5 Pull the plate holder to a position 45 degrees from the center of the imaging stage 6 Close the imaging chamber door and turn on the power to the Lumina XR at the rear main power switch 7 Re initialize the instrument through the Living Image software This moves the plate holder to the home position by locating the limit sensors 70 IVIS Lumina XR Imaging System Manual This page intentionally blank Y Ca AR Index A accessories 61 acquiring fluorescent images 50 51
22. the IVIS Lumina XR is serviced by Caliper Caliper employees are trained in radiation safety However check with your local radiation control authority to determine the specific radiation survey requirements at your facility It necessary have a qualified expert other than a Caliper employee survey the installation before operating the instrument e After any abnormal condition that could impair any of the safety systems For example the light box door becomes difficult to open or close For more information see Conducting the X Ray Radiation Survey page 33 Additionally Caliper recommends a daily or weekly light leak check as described in Maintenance amp Safety Checks page 33 Using the High Reflectance Hemisphere to look for possible light leaks into the imaging chamber may help detect potential radiation leaks out of the chamber Performing this check DOES NOT invalidate the requirement for the 12 month TOTAL radiation survey It is possible for X rays to leak from areas outside of the imaging chamber for example from the electronics enclosure 5 5 Regulatory Compliance Customers in the US are directed to check with their state radiation control program director for registration requirements For a list of U S state agencies and Canadian Provinces see the Safe Operating and Emergency Procedures for the Operation of the IVIS Lumina XR Cabinet X Ray System document This document was provided as part of the pre installation instru
23. their respective owners Apple Macintosh and QuickTime are registered trademarks of Apple Computer Inc Microsoft PowerPoint and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries Adobe and Illustrator are either registered trademarks or trademarks of Adobe Systems Incorporated in the United States and or other countries Intel is a registered trademark of Intel Corporation DIr Y Ca cc 5 X Ray Safety amp Radiation Hazards IMPEQUENOA lt a ic ae aed ees rs Be Sas as Bete hg Be Ses ew iaa 15 Effects of Radigiioh si cc cee cee eRe eka RE eK A 15 X Ray Dose LIMITS so s e mba Gee be Behr dk ee Bod By Sauk Gb a a A hm A 16 Safety Features amp Safety Systems a 4 o 16 Regulatory Compliance lt lt s ss e sesa cacion ae tiua ka naa 17 5 1 Introduction The IVIS Lumina XR produces X rays when the X ray function has been energized and initiated The instrument may also be operated in standard bioluminescence or fluorescence mode without X ray generation The IVIS Lumina XR is defined by most regulatory agencies as a Cabinet X Ray System A cabinet system is one that produces little or no X ray exposure to the user and is safe to operate with the user in close proximity Caliper certifies the Lumina XR to produce not more than 0 5 millirem per hour at a distance of 5 cm from the instrument surface In addition the instrument is certi
24. 34 Cleaning the Optical Components amp Filter Replacement Contact Caliper technical support for information about cleaning or sterilizing any of the optical components or the optical filter replacement 10 7 Fluorescence Hardware Replacement Parts List Item Part No Quartz tungsten halogen lamp for fluorescence light source module 123435 Fuse 3 0A 5x20 250V 123436 Low fluorescent imaging mat 117839 For a complete list of the available options and accessories for the IVIS Lumina XR system see Appendix B page 61 56 IVIS Lumina XR Imaging System Manual This page intentionally blank DIr Y Caliper Appendix A Moving the IVIS Lumina XR With the XWS 260 Workstation Shutting Down the Imaging System 4 57 Moving the Imaging System on the XWS 260 Workstation Starting the Imaging System This appendix explains how to move the IVIS Lumina XR configured with the XWS 260 workstation Figure A 1 CAUTION Caliper recommends that you do not move an IVIS Lumina XR that is not located on the XWS 260 workstation If you need to move an imaging system that is not on the workstation contact Caliper technical support for assistance The procedural steps include e Shut down the system components and unplug them from the line power e Move the workstation with the components e Restart the system components after the power is restored CAUTION The IVIS Lumina XR
25. ED mounts using the nut wrench that is supplied with the filter wheel kit NOTE The left rear screw is difficult to see from the front of the chamber Both screws must be removed before proceeding to step 3 otherwise the plate can cause injury as it becomes unrestrained 3 Remove the two front retraining screws located behind the LED mounts Support the illumination plate assembly with one hand as you remove the last screw 64 IVIS Lumina XR Imaging System Manual 5 Remove the three flathead retaining screws using the T handle tool supplied in the filter kit NOTE At this point the filter wheel is only retained by magnets Wear the cotton gloves provided in the filter wheel kit to avoid putting fingerprints on the filters W Ca i N Chapter C Emission Filter Wheel Replacement Instructions 65 6 Remove the filter wheel from the gear a C 2 Replacing the Filter Wheel 1 Putthe filter wheel onto the gear Fit the two pins on the gear into the slots in the filter wheel Either pin orientation is acceptable Replace the flat head screws and illumination plate Replace the front and rear retaining screws C 3 Installing the Calibration Data 1 Place the Emission Filter Wheel Data CD ROM into the drive and copy the contents of the folder to Living Image Application folder C Program Files Xenogen Living Image 4 x EMFilters NOTE The folder Living Image 3 x refers to the versi
26. HALL NOT BE LIABLE FOR SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES INCLUDING BUT NOT LIMITED TO LOSS OF ANTICIPATED BENEFITS OR PROFITS LOSS OF SAVINGS OR REVENUE PUNITIVE DAMAGES LOSS OF USE OF THE SYSTEM OR ANY ASSOCIATED EQUIPMENT COST OF CAPITAL COST OF ANY SUBSTITUTE EQUIPMENT OR FACILITIES DOWNTIME THE CLAIMS OF ANY THIRD PARTIES INCLUDING CUSTOMERS AND INJURY TO PROPERTY RESULTING FROM THE PURCHASE OR USE OF THE SYSTEM OR ARISING FROM BREACH OF THE WARRANTY BREACH OF CONTRACT NEGLIGENCE STRICT TORT OR ANY OTHER LEGAL OR EQUITABLE THEORY EVEN IF CALIPER KNEW OF THE LIKELIHOOD OF SUCH DAMAGES CALIPER SHALL NOT BE LIABLE FOR DELAY IN RENDERING SERVICE UNDER THE LIMITED WARRANTY OR LOSS OF USE DURING THE PERIOD THAT THE SYSTEM IS BEING REPAIRED Some countries states or provinces do not allow the exclusion or limitation of implied warranties or the limitation of incidental or consequential damages for certain products or the limitation of liability for personal injury so the above limitations and exclusions may 14 IVIS Lumina XR Imaging System Manual 4 2 Patents be limited in their application to you When any implied warranties are not allowed to be excluded in their entirety they will be limited to the duration of the applicable written warranty This Limited Warranty gives you specific legal rights which may vary depending on local law This Limited Warranty shall be governed by the laws of the State of California
27. IS Lumina XR in ANY manner by making any kind of hole or aperture in the instrument or removing any component of the radiation shielding Dr Y Ca asc 3 Warnings Electrical Saloy ezo a a A a Ge ee 9 X Ray DIME 2c ce nee aR SERRE ER RRR ERS ERE REM Re ER ES 9 Eye Safety and Burn Hazard e 10 Mechanical Saleh q a uo Say dk ee A A Sw AR 10 Chemical amp Biological Safety atodi std 11 Panels Cover and Modules 0000 pee eee eee 11 3 1 Electrical Safety VOLTAGE DO NOT attempt to service the IVIS Lumina XR yourself Although there are no voltages in excess of 24V inside the imaging chamber local line voltages 110VAC or 230VAC are present in the lower electronics tray The light source module may be user serviced for line fuse and lamp replacement only There are no other user serviceable electrical parts in the light source module with the exception of the line fuse and tungsten halogen lamp See Troubleshooting page 51 for instructions on these procedures Contact Caliper technical support for other electrical service needs WARNING If necessary wipe exterior surfaces of the light source module with a soft cloth DO NOT use fluids to clean the exterior of the module Do not allow fluids of any kind to enter the light source module under any circumstances See Cleaning the IVIS Lumina XR page 34 WARNING When the power is on DO NOT disconnect or reattach the electri
28. Measured Temperature Does Not Equal the Demand Temperature 9 2 Photographic Image Is Unacceptable 0 02 00 9 3 Luminescent Image Is Unacceptable o o 9 4 No lmage Produced ca sea ca 0 00000 a a a a a a eaaa a e a a 10 Fluorescence Module o eee ee 10 1 Installation Requirements 0 2 0 00022 ee 10 2 Specifications ss scs aoso eee ee a a MR AA a e a 10 3 Description amp Theory of Operation 0 0 2 0 00200000 10 5 Troubleshooting lt as aa c a sanee 0 00002 a a e e a a e a e e oa 10 7 Fluorescence Hardware Replacement Parts List oaaao a a Appendix A Moving the IVIS Lumina XR With the XWS 260 Workstation A 1 Shutting Down the Imaging System 2 e A 2 Moving the Imaging System on the XWS 260 Workstation A 3 Starting the Imaging System 2 a Appendix B IVIS Lumina XR Options amp Accessories Appendix C Emission Filter Wheel Replacement Instructions C 1 Removing the Filter Wheel 2 2 0 0 00 00 0000000000000 C 2 Replacing the Filter Wheel o C 3 Installing the Calibration Data 2 2 0 002 2 000 000 0200 000 Appendix D Removing amp Replacing the Scintillation Plate Holder 67 D 1 Removing the Scintillation Plate Holder o ee 67 D 2 Replacing the Scintillation Plate Hol
29. OFF position and remove the key Turn off the VIS Lumina XR imaging chamber Turn off the computer using the standard Windows shut down procedure vk ON Turn off the power to the other system components and power surge protection devices 6 If moving the system unplug the devices from the wall If you have any problems during the shut down or start up procedure please contact Caliper technical support for assistance NOTE The Emergency OFF switch is not intended as a main X ray source control and should not be used to turn the X ray function ON or OFF on a routine basis It should only be used in the unlikely situation where the X ray source must be immediately turned OFF Under normal circumstances it should be left in the ON position and left as is lt Q Y Ca ec 8 Care 8 Maintenance Surveying the IVIS Lumina XR for Radiation Leakage 4 33 Maintenance amp Safety Checks 1 aa a 33 Cleaning the IVIS Lumina XR aaau a eo 34 Cleaning the Scintillation Plate Window Holder 35 8 1 Surveying the IVIS Lumina XR for Radiation Leakage Caliper recommends and some local government agencies may require an X ray leakage safety test be performed under the following conditions e Every 12 months e When the system is installed at a new site unless the instrument was moved using the XWS 260 workbench and no abnormal mechanical shocks occurred e After Caliper performs mainten
30. WJ IYIS Acquisition Control Panel Imaging Mode Exposure Time Binning FiStop Excitation Filter Emission Filter Temperature Status Field of View fc y X Rays will be produced Demand Measured when energized sece fio si Camera Temp 30 ggo bane Stage Temp 370 4 a Subject height 0 00 Jem SAP ee EEE Focus fuse subject height Temperature M Locked Initialize The temperature square is green when the operating temperature is reached Click the temperature square to display the current temperature Figure 7 1 VIS Acquisition Control panel in the Living Image software 8 Allow the system to initialize You will hear the motors move The System Status box displays the current changes The temperature square in the IVIS System Control panel is red at startup and turns green when the operating temperature is reached The IVIS System Control panel displays the current temperature Figure 7 1 When the temperature is locked at 90 C as indicated by the green light in the System Control panel Figure 7 2 the instrument is ready for operation For operating instructions see the Living Image Software Manual 9 To use the X ray modality a Verify that the Emergency OFF switch is in the ON position b Turn the key selector switch to the ON position c Confirm that the amber arming switch has been pushed and the light is ON d Verify that the X ray source
31. ance or service e After any abnormal condition that could impair any of the safety systems For example the light box door becomes difficult to open or close Conducting the X Ray Radiation Survey A radiation leakage test is a complicated matter requiring sensitive and expensive equipment Some states or localities may require special training and certification to perform the test Contact Caliper technical support for information regarding these tests or for scheduling a Caliper trained person to conduct the survey as part of an overall safety check 8 2 Maintenance amp Safety Checks Daily Safety Checks The following safety checks should be performed on a daily basis 1 Verify that the door interlock discussed in Section 7 4 page 30 is in good repair 2 Verify that the key switch functions properly 3 Verify that the X Ray On red indicator lights are functioning properly when the X ray modality is used The lights are located e On the lower electronics panel e In the camera cover e On the rear of the instrument only visible from the rear of the instrument and is intended to warn service personnel 4 Verify that the amber X ray armed indicator is working 5 Light leak check described on page 41 34 IVIS Lumina XR Imaging System Manual Weekly Safety Checks The following safety checks should be performed on a weekly basis 1 All checks performed on a daily basis 2 Inspect all screws holding the door shi
32. as hose on the right hand side of the stage and move the manifold out of the way The gas hose can be removed from the barbed fitting on the stage or from the right hand barbed elbow on the manifold Figure D 1 Gas tubing connected at Gas tubing connected Manifold gas hose disconnected from the right hand the barbed fitting on stage at the right hand elbow elbow on the gas manifold Figure D 1 Gas manifold connections 3 Using the 5 64 Hex screwdriver remove the two 6 32 buttonhead screws that hold the sheet metal cover Figure D 2 68 IVIS Lumina XR Imaging System Manual a a e Figure D 2 Remove the buttonhead screws from the sheet metal cover 4 After the sheet metal cover is removed rotate the scintillation plate out into the center of the stage by pulling the plate holder forward and then turning it in a counter clockwise direction AN CAUTION CAUTION Avoid putting fingerprints on the window Pull straight forward without bending the paddle up or down Nn Using the 5 64 Hex screwdriver remove the three 6 32 flathead screws that hold the scintillation plate holder and lift the holder away from the drive mechanism Figure D3 The scintillation plate holder and its window can now be cleaned For more details see Section 8 4 page 35 NOTE Support the scintillation plate when the screws are loosened These screws are called flathead but the head of the screw has a bevel with a flat on the top
33. assistance A pe Zu Click DW20010705 122916 Series Plate Test Acq Date Thu Jul 05 2001 Expe rime nt Acq Time 12 29 29 Label Bin M 2 FOV 15 8 Comment Camera IVIS 1356 LN1300EB Analysis Comment Figure 9 2 Condensation example 9 3 Luminescent Image Is Unacceptable Binning f stop and exposure time affect the appearance of a luminescent image Please refer to the Living Image Software Manual for instructions on setting binning exposure time and f stop values In order to function properly and reduce camera noise the CCD camera must be cooled to the demand temperature before acquiring an image If the camera is not cooled to the demand temperature imaging may result in false positive signals Y Caliper F cris Chapter 9 Troubleshooting 41 Problem Corrective Action Light contamination Check to see that there are no extraneous light sources in the imaging Internal chamber Many substances phosphoresce when exposed to light Be especially cautious of plastics and substances that contain pigment Be sure to pre screen any substance or material before performing actual experiments Light contamination A 2 diameter high reflectance hemisphere Caliper part no 118937 is External used to help check for light leaks Figure 9 3 Contact Caliper technical support to purchase this accessory To check for light leaks 1 Place the high reflectance hemisphere in the imaging chamber on the stage usi
34. ay Scintillation Module The main component of the scintillation module is a cesium iodide CsI screen that is used to convert the image shadow produced by the X rays into visible light which can be imaged by the camera The screen is composed of a structured layer of CsI salt on a substrate The screen is housed in a plastic retainer with a protective glass cover After X ray imaging the screen rotates out the way into a protective housing or garage The garage serves two functions 1 prevents incidental afterglow from interfering with other imaging modalities and 2 protects the screen window from contamination such as cleaning liquids CAUTION DO NOT direct strong sprays of cleaning liquids at the scintillation module While reasonably water tight a directed spray could penetrate the seals of the module and damage the sensor WARNING The glass window covering the scintillation sensor is very thin and fragile Use care when cleaning or handling to prevent injury from broken glass See page 35 for recommendations regarding cleaning the scintillation screen 6 4 X Ray System Control Panel The front panel located under the door of the imaging chamber has two switches and two indicator lights associated with the X ray function of the instrument The main ON OFF switch see Figure 7 3 page 29 that controls the electrical power to the full instrument is on the rear of the VIS Lumina XR Activation of this switch provides power to the
35. cal control cable that connects the fluorescence equipment excitation filter assembly to the light source module and the IVIS Lumina XR electronics tray See System Components page 45 for photographs of these components Disconnecting or reconnecting the control cable when the system has electrical power will damage the system Always turn off the switch on the front panel of the light source module and the rear mounted ON OFF switch on the IVIS Lumina XR before you connect or disconnect any of these cable connections 3 2 X Ray Safety AV A YV WARNING This equipment produces X rays when energized WARNING The IVIS Lumina XR should be operated only by personnel who have been trained in radiation safety and the operation and safety instructions contained in this manual Caliper also recommends that personnel who operate the equipment or are close proximity to the equipment use a radiation film badge or other type of appropriate personal dosimeter 10 IVIS Lumina XR Imaging System Manual 3 3 Eye Safety and Burn Hazard The light source module and the connecting fiber optic cables produce intense light that can cause eye damage The module uses a tungsten halogen lamp bulb that operates at a high temperature which if exposed to a user s skin could cause a burn WARNING DO NOT operate the light source module or the fluorescence equipment without all of the fiber optic cables connected at both of their end connections
36. citation filter and fluorescence level as well as standard annotations such as exposure time f stop FOV and date time of exposure 10 5 Troubleshooting Hardware Problems If you have difficulty during fluorescent imaging it may be due to the lack of excitation light Loss of excitation light can result from either a burned out quartz tungsten halogen lamb or a blown line fuse The following procedure describes a troubleshooting process for determining the problem Figure 10 3 page 46 shows the fluorescence light source module 1 Verify that the fiber optic cables are not loosened or disconnected from their proper connectors N Adjust Fluor Light Level excitation lamp to a value of high in the IVIS System Control Panel uy Check the Change Lamp When Red indicator on the front of the light source module If indicator is red proceed to Lamp Replacement page 53 52 IVIS Lumina XR Imaging System Manual 4 Take a fluorescent image and check for light and fan operation by looking through the Nn fan guard on the rear panel of the light source module If there is neither a blown fuse is the probable cause for light loss See Fuse Replacement below Observe the operation of the system by selecting Inspect in the Fluorescent Lamp Level drop down list This causes the selected filter to move into place and the lamp to turn on Open the chamber and visually inspect to see if the excitation light is incident
37. controls the illumination intensity level of the lamp with options Off Low High and Inspect The Low setting is approximately 18 of the High setting Inspect turns on the illumination lamp allowing the user to manually inspect the excitation lamp NOTE Make sure that the filters of interest are selected in the filter popup menus before you select Inspect The inspect operation automatically positions the filters that are selected in the filter popup menus before turning on the lamp Subsequent changes to the filter popup menus will have no effect until another inspect operation is performed A IVIS Acquisition Control Panel TE e ECN Subject height 1 50 Blom Focus use subject height v Temperature EE Locked Figure 10 10 VIS Acquisition Control panel Procedure for Acquiring Fluorescent Images 1 Ifitis not already on start up the acquisition computer and start the Living Image software The System Control panel appears Figure 10 10 2 Click Initialize IVIS system After initialization the Temperature box in the center of the panel should be green indicating that the CCD camera is adequately cooled Allow 10 to 15 minutes for the camera to reach the proper temperature The Temperature box changes from red to green when the CCD camera has reached the proper operating temperature of Y Caliper Chapter 10 Fluorescence Module 51 3 Place the sample to be imaged in the center of the s
38. ctions and is also included on the same CD ROM as this manual International customers should check with their governing bodies about possible registration or other requirements Caliper certifies that the VIS Lumina XR meets the Japanese standard JAIMASO101 2001 with respect to X ray containment Caliper certifies that the VIS Lumina XR complies with FDA regulation CFR 1020 40 after installation at the customer s site Caliper also certifies that the instrument meets all of the International regulations of the country where it is installed 18 IVIS Lumina XR Imaging System Manual This page intentionally blank Dr Y Ca N 6 IVIS Lumina XR Components amp Specifications COD CAMA A AR oe e a 20 X Ray System amp Components a 20 X Ray Scintillation Module gt e ss sss o 22 X Ray System Control Panel 4 2 4 2 26 5 srera tre ras nares 22 Key Selector Switch amp Lost Keys 1 ee 4 o 23 Imaging Chamber a s s a tob 22 ha ee Se be Rae amp Eee a 23 ES 4 a dio ce cd it dd E E A Se Kos oe 25 Optical Filter Wheel a oie s ie eo ea Re oe a a a SS 25 Acquisitian COMPUTES ke cd be EE AR re REE RED A 25 Environmental Requirements 5 4 6 6 6 ew eR RR ee 26 The IVIS Lumina XR is an imaging system that consists of a charged coupled device CCD camera that can image animal subjects primarily mice using three modalities bioluminescence fluorescence and X ray The Lumina XR includes e A cam
39. der 2 2 ee 69 iv IVIS Lumina XR Imaging System Manual This page intentionally blank a Y Ca N 1 Welcome IMA CUEMAN aaa A ARA Re ee E ee 1 Caliper Technical Support o 2 1 1 Introduction The IVIS Lumina XR is a high sensitivity in vivo imaging technology platform that enables noninvasive visualization and tracking of cellular and genetic activity within a living organism through optical and X ray modalities Figure 1 1 Figure 1 1 IVIS Lumina XR system Optical imaging allows the detection of both bioluminescent and fluorescent illuminating sources within an organism by tagging specific proteins within cells or organisms by a gene encoding one of the luciferase enzymes or fluorescent protein that enable bacteria insects and animals to emit light at defined wavelengths Fluorescence allows not only reporter based approaches i e fluorescent protein expression but the ability to target specific molecules within the living organism with the use of fluorescent probes The emitted light corresponds to the number and location of the tagged entities This enables a non invasive approach to observe biological functions the spread of disease and or the effects of a drug throughout an organism The IVIS Lumina XR takes very low light level images that can be saved and displayed for subsequent analysis In addition to bioluminescent and fluorescent imaging the IVIS Lumina XR incorporates pla
40. e right hand barbed elbow on the manifold Figure 8 1 NOTE This step is not necessary but may make it more convenient to clean the plate holder 36 IVIS Lumina XR Imaging System Manual Gas tubing connected Gas tubing connected at Gas manifold gas hose disconnected from the right at the barbed fitting on the right hand elbow on hand elbow the stage the gas manifold Figure 8 1 Gas manifold connections 2 Using the Living Image software service software commands move the scintillation plate to the center of the stage Figure 8 2 Figure 8 2 Scintillation plate moved to the center of the stage 3 Wet a small portion of the soft lint free wipe Caliper part no 126291 with the optical cleaner Caliper part no 123495 Figure 8 3 Y Caliper 4 eet Chapter 8 Care amp Maintenance 37 Figure 8 3 Apply optical cleaner to a soft lint free wipe 4 Starting at the middle of the plate wipe the window surface using a gentle circular motion to remove dirt or smudges Figure 8 4 Figure 8 4 Wipe the window surface 5 To clean the bottom surface of the scintillation plate holder soak a soft wipe in ethyl alcohol and rub the underside of the holder It may be convenient to use a foam paint applicator to support the alcohol soaked wipe Figure 8 5 Support the applicator with the wipe as shown in Figure 8 5 to prevent bending the plate holder A solution of 3 to 5 bleach may be used on the underside of the
41. e 10 2 or imaging performance may be impaired The reflectors surfaces have been surface treated for optimum light diffusion Y Caliper 4 Ber os Chapter 3 Warnings 11 CAUTION The Living Image software controls excitation filter selection Do not manually turn the numbered knob on the Excitation Filter Wheel Assembly see Figure 10 5 page 47 3 5 Chemical amp Biological Safety Normal operation may involve the use of test samples that are pathogenic toxic or radioactive It is your responsibility to ensure that all necessary safety precautions are taken before such materials are used Dispose of all waste materials according to appropriate environmental health and safety guidelines It is your responsibility to decontaminate the VIS Lumina XR before requesting service by Caliper technical support Ask your laboratory safety officer to advise you about the level of containment required for your application and about the proper decontamination or sterilization procedures to follow Handle all infectious samples according to good laboratory procedures and methods to prevent the spread of disease 3 6 Panels Cover and Modules There are no user serviceable components in the lower electronics tray of the VIS Lumina XR Do not remove the electronics tray from the VIS Lumina XR or the cover from the light source module unless you are instructed by and under the supervision of a Caliper technical service representative
42. el of the light Nn nN source module Pull out the fuse drawer The fuse drawer is part of the power entry module and is located directly beneath where the AC line cord plugs in Use a thin bladed screwdriver or penknife blade if necessary Use care not to damage the drawer Remove the blown fuse that is positioned closest to the light source module The second fuse in front of the holder is a spare Discard the blown fuse Examine the spare fuse and verify it is the correct rating See Electrical Power amp Fuses page 44 Place the replacement fuse into the fuse drawer The fuse will work in either direction Fill the spares slot in the drawer holder if another good fuse of correct rating is available Fuse value 3 0A 5 x 20 250V Xenogen part no 20395 8 Push the fuse drawer until it clicks into position a Reattach the AC line cord to the power entry module then to the AC receptacle on the surge protector 10 Return the ON OFF switch to the ON 1 position the red indicator will be visible on the switch Y Calipe a Chapter 10 Fluorescence Module 53 NOTE For the software to control lamp functions the front panel switch on the light source module must be in the ON position 11 Resume normal operation Lamp Replacement WARNING The following procedure can expose the user to hazardous voltages unless the electrical power to the light source module of the fluorescence equipment is com
43. eld and make sure that none are loose 3 Inspect the metal knife edges on the door and the light box for damage such as bending The knife edges keep X rays in the light box and prevent light from entering Monthly Safety Checks The following safety checks should be performed every month 1 All safety checks performed on a daily basis 2 All safety checks performed on a weekly basis 3 Activate the X Ray Emergency Off switch to verify operation All indication of X ray generation should cease when the switch is pushed in NOTE X rays will need to be generated when performing this test 4 Reset the X Ray Emergency Off switch by turning the red knob clockwise The knob should pop out 5 Re arm the X ray source and restart X ray generation from the Living Image software Annual Safety Checks The following safety checks should be performed every 12 months 1 All safety checks performed on a daily weekly and monthly basis 2 A full radiation survey performed by a qualified person 8 3 Cleaning the IVIS Lumina XR Approved Cleaning Solutions The compounds shown in Table 8 1 do not damage the internal finish of the VIS Lumina XR imaging chamber and are suitable for use as cleaners if required Do not use any solution not included in this list In particular avoid strong bases bleach or acids that may potentially damage the unit and compromise its operation 1 IMPORTANT ALERT Do not spray cleaning
44. en not in X ray mode the scintillation plate moves out of the way during other imaging modalities including photographic imaging Specific information including care of the scintillation plate is given in the next section The X ray system schematic is shown in Figure 6 2 CCD camera _ Scintillation plate Movable shelf X ray tube and X ray filter Figure 6 2 IVIS Lumina XR X ray schematic A solenoid driven X ray filter is located near the X ray source The filter screens out un wanted X ray energies from the total radiation spectrum The filter s normal position is over the source but it can be moved out of the way for X ray imaging requiring the full radiation spectrum The Living Image software controls the filter The X ray filter is part of the X ray source enclosed within the electronics drawer and is not accessible or serviceable by the user WARNING DO NOT attempt to access the interior of the electronics compartment underneath the imaging chamber Improper reassembly could result in disruption of radiation shielding Table 6 1 X ray source specifications Item Description High Voltage Potential 20 to 40 kV 22 IVIS Lumina XR Imaging System Manual Table 6 1 X ray source specifications continued Maximum Current 100 mA Anode Type Tungsten Window Beryllium Spot Size 300 mm Cone Angle 46 degrees Field of View 5 7 5 10 cm Filter Aluminum 0 4 mm thick 6 3 X R
45. era e An imaging chamber with controlling electronics e An X ray source and detector including controls and safety systems e Fluorescence Module including excitation and emission filters The system is controlled by a pre configured computer running Living Image software There are no user serviceable parts in the VIS Lumina XR For more details on the fluorescence equipment see Chapter 10 page 43 Figure 6 1 VIS Lumina XR imaging system 20 IVIS Lumina XR Imaging System Manual VOLTAGE While the IVIS Lumina XR can operate at multiple voltages 100 240 VAC users are not permitted to change the input voltage to any of the system components Several internal modifications are required for voltage change when carried out by Caliper technical support If you want to change the operating voltage contact Caliper technical support 1 IMPORTANT OOO ALERT If you modify the IVIS Lumina XR in any way without prior approval from Caliper all warranties that cover this product are void In addition the computer included with the IVIS Lumina XR is specifically configured to run all system related applications Any modification of existing software or hardware voids all warranties If you have any questions please contact Caliper technical support 6 1 CCD Camera The camera is a scientific grade back thinned back illuminated large format CCD manufactured by Andor Technologies Figure 6 2 CCD Camera Features
46. fied to meet all international exposure requirements typically 0 1 millirem per hour and other regulations for where itis sold The Lumina XR meets all US FDA regulations regarding a cabinet X ray system 5 2 Effects of Radiation The IVIS Lumina XR produces ionizing radiation in the energy range from 20 to 40 kilovolts X ray While this energy can be hazardous to the human body the shielded cabinet protects the user or others in the vicinity from any exposure above background The user is responsible for minimizing the total X ray exposure to the individual mouse or other animal subject as part of the total amount of time spent imaging Only individuals who have been trained to operate the equipment should be permitted to use it In some locations government regulations may require that the user have radiation training and be certified WARNING This product produces X rays Do not attempt to open the IVIS Lumina XR door when X rays are being generated as indicated by the two X Ray On lights WARNING Do not modify this product in ANY way Do not drill or modify the shielding panels in ANY way Do not operate the instrument or turn on the source unless all shielding is in place and is in good repair Do not attempt to access the electronics compartment below the imaging chamber Operation of the instrument in a modified condition could result in exposure to X rays Exposure to X rays can cause serious bodily injury or death Refer
47. filter wheel assembly Figure 10 5 on the back of the IVIS Lumina XR imaging chamber using a 1 4 inch diameter fiber optic bundle Figure 10 6 shows a cross section of this excitation filter wheel assembly Light from the input fiber optic bundle passes through a collimating lens followed by a 25 mm diameter excitation filter Twelve filter wheel locations allow you to choose up to eleven excitation filters A light block is provided in one filter slot and is used during bioluminescent imaging to prevent external light from entering the VIS Lumina XR imaging chamber The twelve position excitation filter wheel is motor controlled through the Living Image software Y Caliper Chapter 10 Fluorescence Module 47 Fiber optic cable connector Filter wheel motor Connector for motor control cable Figure 10 5 Excitation filter wheel assembly Fused Silica Collimating Fiber Optic Bundle Input Lenses Fiber Optic e Bundle Excitation Motor Filter Figure 10 6 The cross sectional area of the excitation filter wheel Following the excitation filter a second lens then focuses light into a one quarter inch fused silica fiber optic bundle inside the IVIS Lumina XR imaging chamber Fused silica core and clad fibers are used in this bundle to avoid the generation of autofluorescence in the fiber as is the case with ordinary glass fibers The fused silica fiber bundle splits into four separate legs that deliver filtered ligh
48. ftware Manual 9 To use the X ray modality a Verify that the Emergency OFF switch is in the ON position b Turn the key selector switch to the ON position c Confirm that the amber arming switch has been pushed and the light is ON d Verify that the X ray source is armed e Use the Control panel in the Living Imaging software to turn on the X ray source during an imaging sequence or session I IS Acquisition Control Panel Imaging Mode Exposure Time Binning F Stop Excitation Filter Emission Filter Temperature Status X Rays will be produced when energized Field of View B y Demand Measured _Service_ fic an Camera Temp 90 3 ggo Acquire St Te 1137 0 o Subject height 0 00 em age Temp 3 ee Sequence Setup Focus Juse subject height y Temperature IT Locked Initialize Figure A 3 Control panel Living Image software 60 IVIS Lumina XR Imaging System Manual This page intentionally blank a Y Y Ca Rc ies Appendix B IVIS Lumina XR Options Accessories Table 2 1 Optional equipment and accessories for the IVIS Lumina Part No Item 7837 XBP 24 Black Paper 1 tablet of 24 sheets 8897 XLS 4 Calibrated Light Source 8918 XGI 8 Anesthesia System 120V 8919 XGI 8 Anesthesia System 230V 8957 XGI 8 Anesthesia System 100V 25698 Kit 3 Port Manifold X Ray Compatible plus Accessories 8937 XRH 2 Reflective Hemisphere 8948 XWS 360 WorkStation Comple
49. functions the front panel switch located on the light source module must be in the On position 16 Resume normal operation Dr Y Caliper Chapter 10 Fluorescence Module 55 10 6 Care amp Maintenance of the Fluorescence Equipment Cleaning the Fluorescence Light Source Module If necessary wipe the exterior surfaces of the light source module with a soft cloth WARNING DO NOT use fluids to clean the exterior or interior of the module Do not allow fluids of any kind to enter the light source module under any circumstances Sprays and liquids that come into contact with the light source module or the IVIS Lumina XR may result in damage to the system or electrocution If the fluorescence light source module requires more aggressive cleaning or sterilization contact Caliper technical support Cleaning the IVIS Lumina XR amp Other Components of the Fluorescence Equipment WARNING DO NOT use fluids or moistened towels to clean the any part of the IVIS Lumina XR where electrical or fiber optic cables make connections Do not use fluids of any kind in the vicinity of the Excitation Filter Wheel Assembly mounted on the rear of the imaging chamber Turn off electrical power to the IVIS Lumina XR before engaging in cleaning operations using fluids The Imaging Chamber power switch is located in the rear on the electronics tray For more details on how to clean the imaging chamber see Cleaning the IVIS Lumina XR page
50. g 7 servicing the equipment 9 shut down procedure 32 specifications computer 26 computer monitor 26 electrical power 44 environmental 44 fuse 44 humidity 44 imaging chamber 24 lamp 44 optics 25 temperature 44 stage curtain 31 start up 27 28 58 59 startup restarting after power outage 29 Y Ca AR subject placement 31 system cleaning 34 35 environmental requirements 26 moving with XWS 260 workstation 57 59 restarting after power outage 29 shut down 32 start up 27 28 58 59 system components Fluorescence Module 45 X ray system 20 22 T technical support 2 temperature 44 measured unequal to demand 39 trademarks 14 troubleshooting camera noise 41 measured and demand unequal 39 no image produced 41 unacceptable luminescent image 40 unacceptable photographic image 40 V ventilation requirements 44 voltage requirements 43 W water and moisture safety 6 Xx X ray dose limits 16 effects 15 hazard regulations 4 5 safety 15 17 safety symbol 4 safety symbol Canada 4 scintillation module 22 source specifications 21 system amp components 20 22 system control panel 22 23 X ray modality 28 59 XWS 260 workstation 57 59 Index 73 73 74 IVIS Lumina XR Imaging System Manual
51. hat is not blocked properly by the filter and is detected by the camera Dr Y Caliper Chapter 10 Fluorescence Module 49 Separation 20 nm Emission Optical Density Wavelength Figure 10 9 Illustration of typical excitation and emission curves The vertical axis in Figure 10 9 is optical density defined as OD log T where T is the transmission An optical density of 0 indicates 100 transmission whereas OD7 indicates a reduction of the transmission to 10 7 Typical transmission of a filter in the bandpass region is about 0 7 ODO 15 and typical blocking outside of the bandpass region is about OD7 The band gap between the two filters is usually defined as the gap at 50 transmission ODO 3 There is a slope in the transition region from bandpass to blocking as indicated in Figure 10 9 A steep slope is required to avoid overlap between the two filters The fluorescence filters are high quality interference filters constructed from alternating layers of dielectric films on a substrate of low autofluorescent glass Care has been taken to minimize autofluorescence of the filter so that its level is below OD7 Filter passbands for the standard set are listed in Table 10 1 Table 10 1 Standard filter sets fluorescent dyes and proteins used with the IVIS Lumina XR Fluorophore Standard High Resolution Emission Filter Options Excitation Filter Set Built In GFP YFP and PKH26 500 Serie
52. he me Bd A Se Od we On Gwe 4 X Ray Safety amp Hazards Regulations 4 4 Environmental Considerations for the System Components 5 Cleaning or Moving the System Components 00 ee eee 6 Power Considerations e o s soe se onerosa eR we we ee es 7 o 5 4h ade te A Bs A ea ee ee A Be me a es Be 7 Other EQUIpment s s cr ee RR EKER EOE stered kerer teni wa 8 2 1 Safety Information This manual provides safety information in the following formats CAUTION A caution note indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury and or mechanical damage It is also used to alert you to unsafe practices It reminds you that all safety instructions should be read and understood before installation operation maintenance or repair of this instrument When you see this symbol pay particular attention to the safety information presented Observance of safety precautions will help avoid actions that could damage or adversely affect the performance of the IVIS Lumina XR If the equipment is used in a manner not specified in this manual the protection provided by the equipment may be impaired WARNING Used when an action or condition may potentially cause serious personal injury or loss of life Mechanical damage may also result VOLTAGE Provides safety information about high voltage or risk of electric shock 4 IVIS Lumina XR I
53. hesia 29 IVIS Acquisition Control panel 50 72 IVIS Lumina XR Imaging System Manual K key selector switch 23 L lamp 44 lamp replacement 53 54 light block 46 Light Source Module 45 dimensions 44 weight 44 lightning 7 limited warranty 13 14 Living Image software 46 low fluorescent imaging mat 55 Lumina options and accessories 61 luminescent image unacceptable 40 M maintenance amp safety checks 33 34 manual information 4 mechanical safety 10 modules 11 moving the system 57 59 N no image produced 41 O optic specifications 25 optical density 49 optical filter wheel 25 options 61 other equipment 8 P panels 11 patents 14 photographic image unacceptable 40 power cord 7 power line surges 7 power outage restarting the system 29 power outages 7 power overloading 7 power sources 7 R radiation survey for leakage 33 radiation effects 15 radiation hazards 15 17 regulatory compliance 17 replace filter 55 fuse 52 53 lamp 53 54 replacement parts fuse 55 low fluorescent imaging mat 55 quartz tungsten halogen lamp 55 requirements installation 43 voltage 43 restarting after power outage 29 S safety chemical amp biological 11 electrical 9 electrical safety 9 eye hazard 10 mechanical 10 panels cover and modules 11 safety features amp systems 16 17 safety instructions 3 8 safety symbols hazardous voltage 4 X ray warning 4 X ray warning Canada 4 scintillation module 22 servicin
54. ight position removable optical filter emission wheel and a 12 position excitation filter wheel e High efficiency lens assembly e Sample illumination system e f stop control e Heated and regulated sample shelf temperature to reduce stress on an animal under anesthesia e Gas anesthesia manifold including gas delivery and exhaust plumbing e Software controlled field of view f stop focus and optical filter wheel Imaging Chamber Specifications IVIS Lumina XR Imaging Description Chamber Power requirements 4 0 A max at 120 V 2 0 A max at 240 V 50 60 Hz Dimensions 19 x 28 x 39 48 cm x 71 cm x 100 cm Door opening dimensions 15 x 20 25 38 cm x 51 cm Weight 160 lbs 160 Ibs 73 Kg A Y Caliper Chapter 6 IVIS Lumina XR Components amp Specifications 25 6 7 Optics Optics Specification Lens f stop f 95 1 16 Field of View Fluorescence or bioluminescence mode A 5cm B 7 5cm2 C 10cm2 and D 12 5cm2 X ray mode A B and C 6 8 Optical Filter Wheel The imaging chamber has an 8 position computer controlled optical filter wheel that is located at the top of the imaging chamber in front of the imaging lens The system has four emission filter set options The first is a the standard GFP DsRed Cy5 5 and ICG Four other emission filter wheels with more narrow band filters are available as options See Appendix B page 61 for ordering information regarding these optional emission filter wheel
55. ing system captures quantifies and images the light emitted by a sample The supplementary X ray capability allows additional anatomical information 7 1 Starting the Lumina XR Imaging System NOTE All components of the IVIS Lumina XR should be left on at all times Periodically rebooting the computer is permissible and does not affect the camera operation 1 Plug the devices into the wall sockets in the new location 2 Turn on the power surge protection devices 3 Turn on the computer and monitor 4 Turn on the VIS Lumina XR imaging chamber the power switch is located on the back of the unit and verify that the other components such as the camera power supply X ray controller both on the back of the unit and fluorescence lamp are also turned to the On position Nn After the desktop screen is displayed start the Living Image software 6 Enter a User ID up to three letters when prompted then click Done 7 To initialize the system click Initialize IVIS System in the System Control panel Figure 7 1 28 IVIS Lumina XR Imaging System Manual IVIS Acquisition Control Panel Imaging Mode Exposure Time Binning F Stop Excitation Filter Emission Filter Field of View fc y Service fio cm Subject height 0 00 3 cm Focus use subject height IE Locked System Status Idle X Rays will be produced en energized Acquire Sequence Setup Initialize
56. instrument but does not permit energizing the X ray source unless the following conditions have been met 1 The imaging chamber door is completely closed and the door handle is in the completely locked position N The Emergency OFF switch is in the ON out position See the note below The key selector switch is turned ON The amber switch has been pushed and the light is ON indicating that all safety interlocks are functioning properly The X ray source cannot be energized from the Living Image software until these conditions have been fulfilled Dx Y Caliper Chapter 6 IVIS Lumina XR Components amp Specifications 23 NOTE The Emergency OFF switch is not intended as a main X ray source control and should not be used to turn the X ray function ON or OFF on a routine basis It should only be used in the unlikely situation where the X ray source must be immediately turned OFF Under normal circumstances it should be left in the ON position and left as is a Figure 6 3 IVIS Lumina XR front panel 6 5 Key Selector Switch amp Lost Keys X ray safety regulations require controlled access to the IVIS Lumina XR The objective of this requirement is to prevent untrained and unauthorized personnel from operating the X ray functionality of the instrument The key operated switch on the IVIS Lumina XR fulfills this requirement when used in conjunction with the user s own written radiation safety procedures The con
57. is armed e Use the Control panel in the Living Imaging software to turn on the X ray source during an imaging sequence or session I IS Acquisition Control Panel Imaging Mode Exposure Time Field of View B y service fio cm Subject height 0 00 cm Focus fuse subject height y Binning F Stop Temperature Status Excitation Filter Emission Filter Demand Camera Temp 30 3 Stage Temp 37 0 X Rays will be produced Measured when energized gy Acquire p Sequence Setup Temperature Locked Initialize Figure 7 2 Control panel Living Image software D Y Caliper Chapter 7 Operating the IVIS Lumina XR 29 7 2 Restarting the System After a Power Outage If the IVIS Lumina XR experiences a loss of supply power turn off the power switch on each component Do not restart the system until reliable line power has been restored Turn off all components and wait until stable line power has been restored 1 Turn on the computer 2 Turn on the imaging chamber 3 Start the Living Image software and click Initialize IVIS System in the IVIS System Control panel 4 For X ray mode the system will need to be re armed by pressing the amber arming switch on the front panel 7 3 Gas Plumbing Anesthesia gas tubing is built into the VIS Lumina XR imaging chamber On the back of the imaging chamber are 0 25 hose barbs that are marked GAS IN and GAS OUT Figure 7 3
58. m Pay close attention to receptacles and to points of connection between cords and equipment Lightning amp Power Line Surges Power Outages Overloading 2 8 Servicing The IVIS Lumina XR is supplied with a surge protector All components should be connected to this device to protect against electrical transient events Failure to isolate the camera from electrical transients may result in damage to the CCD camera If the IVIS Lumina XR experiences a loss of supply power turn off the power switch for all components and do not restart the system until reliable power has been restored WARNING Do not overload wall outlets extension cords or integral convenience receptacles as this can result in a risk of fire or electric shock For more details on the power requirements of the IVIS Lumina XR equipment see Chapter 6 page 26 Facilities should be adequately wired according to local building codes Refer all servicing to Caliper technical support If the IVIS Lumina XR is damaged and requires service unplug the IVIS Lumina XR from the outlet and contact Caliper technical support Servicing by anyone other than an authorized Caliper representative voids the warranty covering the IVIS Lumina XR 8 IVIS9 Lumina XR Imaging System Manual 2 9 Other Equipment Use of any equipment other than that recommended by this manual has not been evaluated for safety and therefore is the sole responsibility of the user Do not modify the IV
59. maging System Manual 2 2 Safety Symbols Table 2 1 shows safety symbols that are found on the VIS Lumina XR and in this manual Table 2 1 Safety symbols Symbol Definition Warning Hazardous voltage Warning The equipment produces X rays when energized Y Warning Canada The equipment produces X rays when energized 2 3 Instructions WARNING The IVIS Lumina XR should be operated only by personnel who have been trained in radiation safety and the operation and safety instructions contained in this manual Caliper also recommends that personnel who operate the equipment or are close proximity to the equipment use a radiation film badge or other type of appropriate personal dosimeter Read Instructions Read and understand all the safety and operating instructions before you install operate or perform maintenance on the IVIS Lumina XR Make sure that you fully understand the following safety instructions warnings and disclaimers before proceeding to the rest of the manual Retain Instructions Retain the safety and operating instructions for future reference Follow Instructions Follow all operating and handling instructions Failure to follow operating or handling instructions may void any warranty covering this product Heed Warnings Abide by all warnings on the product and in the operating instructions Failure to adhere to warnings or safety precautions may void any warranty covering the IVIS Lu
60. mina XR 2 4 X Ray Safety 8 Hazards Regulations This equipment produces X rays when energized Before operating the equipment read and understand the specific information in X Ray Safety amp Radiation Hazards page 15 A Y Caliper Chapter 2 Important Safety Instructions 5 DO NOT operate the IVIS Lumina XR unless an X ray safety survey has been performed within the last 12 months For more information please contact Caliper technical support An X ray safety survey must be performed when the instrument is installed or after it has been moved unless the instrument was moved on the XWS 260 Workstation cart and no abnormal mechanical shocks occurred A survey is also to be performed when the IVIS Lumina XR has undergone any form of service in which the electronics drawer has been opened the safety interlocks have been adjusted or any of the shielding has been removed and re installed After servicing if the safety interlocks are not operating properly or if the X ray shielding is not properly re installed serious injury can result when operating the system Conducting an X ray safety survey is the only way to confirm proper shielding and interlock operation WARNING For radiation survey of the IVIS Lumina XR please comply with your own laboratory radiation regulations or contact Caliper technical support for further assistance Owners and operators of the IVIS Lumina XR are responsible for complying with all regulations in the
61. nar X ray equipment that enables automated X ray co registration to optical images The IVIS Lumina XR is an integrated imaging system that includes 2 IVIS Lumina XR Imaging System Manual e A scientific grade thermoelectrically cooled CCD camera mounted on a light tight imaging chamber with integrated optical modules e The module for X ray imaging e The Living Image Software for automated image acquisition post processing and data analysis e A Windows based computer system for data acquisition and analysis This manual explains how to operate and maintain the equipment and provides guidelines for obtaining the best bioluminescent and fluorescent images Before using the IVIS Lumina XR please read this manual carefully to obtain safe optimum performance and a maximum service life from the unit For instructions on how to acquire fluorescent images using the Fluorescence Module please see Chapter 10 page 43 For instructions on using the Living Image software please see the Living Image Software User s Manual If you have questions regarding this manual or the IVIS Lumina XR please contact Caliper technical support 1 2 Caliper Technical Support Telephone 1 877 522 2447 US 1 508 435 9500 E mail tech support caliperLS com Fax 1 508 435 3439 Dr Y Ca EN 2 Important Safety Instructions Safety ld E IAE 3 Safety Symbols sa ck RRR RRR RRR RRR RR EERE ER 4 HOSP ONS sa ee wm oa o A ee Ee Be Sr
62. ng a subject height of 3 5 cm at field of view D 2 Take a luminescent image of the hemisphere using the luminescent settings f stop 1 Binning Large high sensitivity and exposure time 5 minutes If the hemisphere can be easily seen there is a light leak Contact Caliper technical support for assistance Camera noise Verify that the camera is cooled to the demand temperature 1 Check the measured temperature in the IVIS System Control panel to ensure that it is locked If the camera temperature is locked the camera temperature box is green 2 If the camera temperature box is red click the red box to display the actual temperature See Measured temperature is warmer than the demand temperature page 39 Figure 9 3 High reflectance hemisphere Wear powderless gloves when handling the hemisphere to prevent surface contamination that may glow when using the IVIS Lumina XR 9 4 No Image Produced If no image is produced there may be an error in the Living Image software a problem with the physical connections to the camera or a hardware failure 1 Close the Living Image software and restart the computer 2 Restart the Living Image software and try to acquire an image If after restarting the computer you are still unable to produce an image contact Caliper technical support for assistance 42 IVIS Lumina XR Imaging System Manual This page intentionally blank a Y Y Ca EN 1 0 Fluorescence M
63. nning FiStop Excitation Filter Emission Filter Imaging Mode Exposure Time Binning F Stop Excitation Filter Emission Filter Temperature Status Field of View fc y Field of View fc y System Status X Rays will be produced X Rays will be produced ath eh en energized Demand Measured n energiz R Idle Service fio cm Acquire Service fio cm Camera Temp 20 3 ggo Acquire Stage Temp 370 4 0 Sequence Subject height 0 00 Jem Sequence Setup Subject height fo oo Jem ENE ee Sequence setup Initialize Focus fuse subject height Temperature M Locked Initialize Focus use subject height y a Locked The temperature square is green when the operating temperature is reached Click the temperature square to display the current temperature Figure A 2 IVIS Acquisition Control panel in the Living Image software 8 Allow the system to initialize You will hear the motors move The System Status box displays the current changes The temperature square in the IVIS System Control panel is red at startup and turns green when the operating temperature is reached The IVIS System Control panel displays the current temperature Figure A 2 When the temperature is locked at 90 C as indicated by the green light in the System Control panel Figure A 3 the instrument is ready for operation For operating instructions see the Living Image So
64. ns on how to use the Living Image software that controls fluorescent image acquisition see the Living Image Software Manual that is provided with the software The Schott Fostec DCR III Direct Current Regulated Light Source User s Manual and Technical Reference is a separate manual for the light source module It provides additional useful information on the light source module and its safe operation 10 1 Installation Requirements The fluorescence equipment requires 100 240 VAC electrical power The system automatically accepts the required voltage 1 IMPORTANT ALERT The fluorescence equipment operates at the same voltage as the IVIS Lumina XR chamber and must not be used at other than its labeled voltage For more details on the Fluorescence Module operating requirements see Specifications page 44 44 IVIS Lumina XR Imaging System Manual 10 2 Specifications Electrical Power amp Fuses Voltage Available 100VAC 50 60 Hz 240VAC 50 60Hz Power Consumption 200 W NOTE For more on additional electrical power requirements of the IVIS Lumina XR see Chapter 6 page 19 Environmental Temperature amp Humidity Temperature 15 25 C Humidity 0 to 80 Non Condensing Type of Use Indoor Lamp and Fuse Lamp 150 W Quartz Tungsten Halogen 21 V EKE lamp life 400 hrs High setting 6 000 hrs Low setting Color temperature 3250 K Fuse 3A 10 250VAC Ventilation Requirements Pr
65. nsor assembly Rinse surfaces using a wipe saturated with sterile deionized water Do not allow puddles of water to remain on the surfaces To avoid any phosphorescence from the cleaner be sure that the surfaces are dry before using the imaging chamber Be careful not to smudge the camera lens and optical filters The anti reflective coated glass window on the scintillation module may be cleaned using an Optical Cleaning solution Caliper PN 123495 and lint free wipes Caliper PN 123740 Extreme care should be exercised because the window is very thin and fragile Consider dedicating an IVIS Lumina XR for immunodeficient animals to remove the risk of cross contamination 8 4 Cleaning the Scintillation Plate Window amp Holder The scintillation plate is protected by a glass window with anti reflection coating This window may be cleaned using the optical cleaner and soft absorbent wipes provided in the Scintillation Plate Maintenance Kit Window cleaning may be accomplished without removing the scintillation plate holder but rotating it to the center of the stage enables access Plate rotation is performed using the service utility in the Living Image control panel No tools are required for simple window cleaning other than the optical cleaner and soft wipes 1 Disconnect the manifold gas hose at the right side of the stage and move the manifold out of the way The gas tube can be removed either from the barbed fitting on the stage or from th
66. nt such as the IVIS Lumina XR Typical excitation and emission fluorophore spectra are shown in Figure 10 8 along with idealized excitation and emission filter transmission curves shown as rectangles The excitation and emission filters are called bandpass filters ideally they transmit all the wavelengths within the bandpass region and block absorb or reflect all wavelengths outside the bandpass This spectral band is like a window characterized by its central wavelength and its width at 50 peak transmission full width half maximum FWHM Excitation Emission Excitation Emission Filter Filter Transmission Wavelength nm Figure 10 8 Typical excitation and emission spectra for a fluorescent compound Included in the picture are two idealized bandpass filters that would be used with this fluorescent compound Real filters have some leakage outside of the bandpass region and can also exhibit autofluorescence depending on the materials used in the filter construction More realistic filter transmission curves are shown in Figure 10 9 Referring to Figure 10 8 we can define several terms Bandgap the spacing between the transmission regions of the excitation and emission filters Transmission the fraction of light that passes though the filter bandpass region Blockage the light rejection in the non transmitting region or the region outside the band of the filter spectrum Leakage undesirable light t
67. ntrol panel under the Focus drop down menu select the Manual Focus option The software displays a Manual Focus Window of the subject 32 IVIS Lumina XR Imaging System Manual 3 If the subject is not centered open the door and reposition the subject Click the Update button to refresh the image Repeat this step until the subject is properly centered 4 When the subject is centered properly click the Done button on the Manual Focus Window to close the window For more details Living Image Software User s Manual Glowing Materials Always keep in mind that nearly EVERYTHING glows that is has the potential to phosphoresce and contaminate the image Most plastics almost all tape plants paint rodent food mostly plants mouse urine and animal bedding have been found to glow Use caution when introducing materials into the VIS Lumina XR It is advisable to pre screen all items by imaging them alone before imaging them with samples under study Caliper recommends using non powdered gloves when working with IVIS Lumina XR equipment 7 6 System Shut Down Procedure Caliper does not recommend power cycling the IVIS Lumina XR turning the system components on and off If it is necessary to shut down the imaging system for any reason it is important to follow the procedure below 1 Close the Living Image software and save any information of interest at the prompt Return the X ray ON key selector switch to the
68. o 3 2 1 Safety Information s s s e d s coe Gee eR A A o e 3 2 3 Instructions scor w a Poe a eee aaa ar E Bae amp 4 2 4 X Ray Safety amp Hazards Regulations 20 00 0000 00004 4 2 5 Environmental Considerations for the System Components 5 2 6 Cleaning or Moving the System Components 2 2004 6 2 7 Power Considerations s se cs ca mawena ad ajed aa aie A a a a a 7 2 8 Servicings 2 6463 setta bw eee eS ea ER ee A a e E 7 3 WamMminGS occiso HERES HE YRS ES HES A EOS RGS SHS 9 3 1 Electrical Safety vcs w ada de Go Wa a a a RA ee 9 32 X Ray SaS sone ase oe Ge RR A Sse a er he 9 3 3 Eye Safety and Burn Hazard 2 2 e 10 3 4 Mechanical Safety iec a eia oes sece ee ea w E e a e e Ga e 10 3 5 Chemical amp Biological Safety oaoa 20 2 000 200 0020 00020000008 11 3 6 Panels Cover and Modules 2 a a a a 11 4 LegaliNGuces y cce aae bee AR a a wee AS 13 4 1 Limited Warranty 13 4 2 Patents 28 4 cestas ae a BA ee ee oe Oe eae 14 4 3 Trademarks 24 2 6 aca d amp rss OM aoe we AMA RO OR eS we eee we BG 14 5 X Ray Safety amp Radiation Hazards o a 15 3 1 Introduction ed Peek ee ee ee be ea ee ek ae ede Rope Ga ad 15 5 2 Effects of Radiation 4 230224 ca ba teen oe boa dw doe ed Be wa De we ws 15 5 3 X Ray Dose Limits a oa csser ese bente cas dradu dto tatws 16 5 4 Safety Features amp Safety Systems oaa aa a 16 5 5 Regulatory Compliance
69. odule Installation Requirements e 43 SPECWiCAtiIGNe asc crio AA 44 Description amp Theory of Operation 2 2 e o e 45 Acquiring Fluorescent Images lt lt um ss eds aa ee 50 Troubleshogting 2 0 ha a AA A AA es 51 Care amp Maintenance of the Fluorescence Equipment 4 55 Fluorescence Hardware Replacement Parts List 55 The Fluorescence Module provides the VIS Lumina XR with fluorescent imaging capability The fluorescence equipment can be used for in vitro or in vivo applications The sensitive range of the VIS Lumina XR CCD camera sets the wavelength range for fluorescence applications which is approximately 400 950 nm As with bioluminescent imaging wavelengths greater than 600 nm are preferred for in vivo fluorescent applications due to lower absorption in tissue The Living Image software controls fluorescent image acquisition including lamp power level and filter selection This chapter explains how to operate the fluorescence equipment with the IVIS Lumina XR It also provides important safety and maintenance information CYIMPORTANT ALERT To ensure optimum and safe performance of the Fluorescence Module with maximum service life read this chapter carefully before you use IVIS Lumina XR with the Fluorescence Module You should also be familiar with and refer to the other chapters of this manual for the VIS Lumina XR For instructio
70. on of Living Image you are running If your computer has more than one version copy the contents of the CD ROM into all versions of Living Image 2 Re initialize the camera system The new filter wheel wavelengths are now available in the filter wheel selection 66 IVIS Lumina XR Imaging System Manual This page intentionally blank DIr Y Ca ee ise Appendix D Removing amp Replacing the Scintillation Plate Holder Removing the Scintillation Plate Holder 0 2 000008 67 Replacing the Scintillation Plate Holder 00 0050 ae 69 There may be occasions when the scintillation plate holder has to be removed for replacement or for a more thorough cleaning than can be accomplished in situ This appendix explains how to remove the holder or paddle For this procedure you will need the 5 64 Hex screw driver from the Scintillation Maintenance Kit PN 126373 that was provided with the IVIS Lumina XR imaging system This kit also contains some additional screws in case some are lost when conducting this procedure If screws are lost inside the imaging chamber you should try to recover them so they do not cause injury to the mechanisms under the stage D 1 Removing the Scintillation Plate Holder 1 Close the Living Image software and turn off the power to the VIS Lumina XR at the main power switch that is located at the rear of the instrument near the power cord 2 Disconnect the manifold g
71. on or failure to confirm to published specifications during normal usage in accordance with the instructions in this manual Repairs and replacements under the Limited Warranty will be made at Caliper s expense Caliper s limit of liability under the Limited Warranty shall be the purchase price of the Imaging System Caliper shall not be liable for any other losses or damages These remedies are the Customer s exclusive remedies for breach of this Limited Warranty No coverage or benefits shall be provided under this Limited Warranty if any of the following conditions apply a The System has been subjected to unauthorized modifications e g unauthorized installation of hardware or software unauthorized repair or servicing misuse neglect abuse accident alteration any use inconsistent with or in contradiction to the instructions in this manual or other acts which are not the fault of Caliper b Caliper was not advised in writing by the Customer of the alleged defect or malfunction of the System within the earlier to occur of ten 10 days after the expiration of the Limited Warranty period or 15 days after becoming aware of the defect or malfunction iv Ifa problem develops during the Limited Warranty the Customer shall contact Caliper vi me technical support for assistance THE FOREGOING LIMITED WARRANTY IS THE CUSTOMER S SOLE AND EXCLUSIVE REMEDY AND IS IN LIEU OF ALL OTHER WARRANTIES EXPRESS OR IMPLIED CALIPER S
72. ovide sufficient space minimum 6 inches behind the fan of the light source module so that airflow is unobstructed Provide a similar minimum distance behind the IVIS Lumina XR to enable fan cooling as well as adequate room for fiber optic cable routing Chemicals Required for Operation No chemicals are required for the operation of the IVIS Lumina XR or the fluorescence equipment Other user supplied chemicals or materials may be required for your specific biological testing procedures Weight amp Dimensions of the Fluorescence Light Source Module Weight 4 94 lbs 2 24 kg Depth 8 61 inches 21 9 cm Width 7 27 inches 18 5 cm Height 4 6 inches 17 7 cm D Y Caliper Chapter 10 Fluorescence Module 45 10 3 Description amp Theory of Operation System Components The Fluorescence Module provides fluorescent imaging capability You can conveniently switch between bioluminescent and fluorescent imaging applications Figure 10 1 shows the fluorescence equipment and Figure 10 2 depicts the interior of an IVIS imaging chamber green indicates the excitation light Excitation filter Imaging chamber wheel assembly Control cable Fiber optic cable Lightsource module Figure 10 1 VIS Lumina XR and fluorescence equipment CCD camera Emission filter wheel assembly Excitation filter wheel assembly aeei eflectors Light source module sail Figure 10 2 IVIS and fluorescence equipment
73. plate holder if care is used not to get it on the AR coated window 38 IVIS Lumina XR Imaging System Manual NOTE If this method does not completely clean the plate holder it may be necessary to remove the plate holder from the drive mechanism for cleaning Instructions on how to remove and clean the plate holder are detailed in Appendix D page 67 Y Calipe AMN 9 Troubleshooting Measured Temperature Does Not Equal the Demand Temperature 39 Photographic Image ls Unacceptable 4 40 Luminescent Image ls Unacceptable 40 Na Image Produced 4302 4 0 S24 e465 Gawd baw eG eee Ge ewe OT 9 1 Measured Temperature Does Not Equal the Demand Temperature At start up the Living Image software programs the CCD camera to maintain the CCD at 90 C If the camera power supply remains on IVIS Lumina XR box the system maintains this temperature regardless of whether the Living Image software is open or the computer is turned on m Tocheck the temperature of the CCD click the Temperature square red or green in the VIS System Control panel of the Living Image software Figure 9 1 WJIYIS Acquisition Control Panel IVIS Acquisition Control Panel 1x Imaging Mode Exposure Time Binning F Stop Excitation Filter Emission Filter Imaging Mode Exposure Time Binning F Stop Excitation Filter Emission Filter Field of View B y Temperature Status Field
74. pletely eliminated As instructed in the procedure turn off the lamp from the module front panel ON OFF switch and remove the electrical power to the module by disconnecting the plug from the surge protector and the back of the module WARNING Before you replace the tungsten halogen lamp or lamp assembly in the fluorescence light source module verify that the lamp and socket are cool before proceeding 1 Review the sections Lamp Module Replacement and Lamp Replacement in the separate manual for the fluorescence light source module SCHOTT Fostec DCR III Direct Current Regulated Light Source User s Manual and Technical Reference It contains additional useful information and warnings regarding the light source N Inthe IVIS System Control panel of the Living Image software put a check mark next to Fluorescent and set the Lamp Level to Off Figure 10 11 Allow the fan on the light source module to run for several minutes until the fiber optic connector nosepiece with thumb screw is cool to the touch j IVIS Acquisition Control Panel D CO OMITE EN STEIN CEM O a a ECM a System Status ide sore E Blen Sa O Figure 10 11 VIS Acquisition Control panel 3 Press the ON 1 OFF 0 switch to the OFF 0 position on the light source module 4 Remove AC line cord from the AC power receptacle on the surge protector and from the back of the module 5 Release the lamp assembly from the front panel of the light
75. raph imaging parameters are automatically controlled and generally produce a good quality photo If shiny objects are imaged creating specular reflections the automatic algorithm may get confused and produce an underexposed image Also refer to the Living Image Software Manual for further details on acquiring images Problem Possible Cause Image is streaked Subject moved during the exposure Corrective Action Check to see if the subject may have moved If the subject is not on the sample stage it is probably on the floor of the imaging chamber If the sample has moved locate and reanesthetize it If gas anesthesia is being used confirm that the anesthesia is turned on and the flow rate is appropriate Image is blurry Subject height is significantly less than or greater than 1 5 cm Incorrect f stop setting The focus is set for a sample height of 1 5 cm Significant deviation from this height results in an out of focus photograph The f stop for photographs should be set to f 8 or f 16 An f stop smaller than 8 reduces the depth of field in the photograph A white spot appears in the center of the field of view An excessively moist environment in the imaging chamber can result in condensation on the CCD window Figure 9 2 Turn off the entire system and remove excess moisture in the imaging chamber Allow the chamber to thoroughly dry If the problem persists contact Caliper technical support for
76. rnational public safety standards and regulations 5 4 Safety Features amp Safety Systems The IVIS Lumina XR is enclosed within shielding that limits X ray exposure to normal background levels The access door to the imaging chamber is provided with two safety interlocks which cut power to the X ray source if they are interrupted Additionally the door is provided with a solenoid activated lock that prevents the door from being opened during an imaging session when X rays could be generated For reasons discussed in the warning above it is important not to attempt defeat of any of these safety features Caliper will perform at least two safety tests on every system at the time of installation e At the time of manufacturing e At the user s laboratory or facility The safety test includes but is not limited to an X ray radiation leakage test Caliper recommends and some local government agencies may require an X ray leakage safety test be performed under the following conditions e Every 12 months e When the system is installed at a new site unless it has been rolled there on the XWS 260 workbench and no jarring shocks have occurred during transport e After a Caliper technician performs maintenance or service in which case the safety survey will be conducted by Caliper ya Y Caliper _ Chapter 5 X Ray Safety amp Radiation Hazards 17 WARNING A Caliper employee will conduct a radiation leakage survey and safety tests after
77. s Low Range 500 520 540 560 580 600 and 620 nm DsRed and Tomato 430 465 500 570 605 640 675 600 Series Mid Range 580 600 620 640 660 680 and 700 nm 710 745 CY 5 5 XenoLight 680 Mid High Range 640 660 680 Katushka Cherry FP 700 720 740 and 760 nm Indocyanin Green and 700 Series High Range 720 740 XenoLight 750 770 760 780 800 820 and 840 nm Multiple Fluorophores Standard Emission Filter Set 515 Spanning 500 900 nm 575 575 650 695 770 810 875 nm Broad Imaging Solution 20 nm bandpass emission filters 35 nm bandpass excitation filters 50 IVIS Lumina XR Imaging System Manual 10 4 Acquiring Fluorescent Images NOTE This chapter provides a quick start guide to acquiring fluorescent images See the Living Image Software User Manual for complete details IVIS System Control Panel Overview Acquiring fluorescent images using Living Image software is controlled through the VIS Acquisition Control Panel Figure 10 10 To acquire a fluorescent image the user must check the Fluorescent box on the left side of the panel Once selected controls for the illumination lamp Fluor Lamp Level and Filter Lock will appear in the top half of the panel Checking the Filter Lock box ensures that the excitation and emission filters are properly paired During image acquisition the lamp is computer controlled through Living Image software The Fluorescent level pull down menu
78. t to four reflectors located on the ceiling of the VIS Lumina XR imaging chamber Figure 10 2 page 45 Typical illumination profiles for stage locations A D fields of view 5 12 cm respectively are shown in Figure 10 7 Note that the profiles for all the stage locations are peaked near their center The non uniformity of the illumination pattern is compensated for in Living Image when units of efficiency are selected for more details see the Living Image Software User s Manual When imaging 96 well plates the lower stage positions C and D are preferred to minimize shadowing effects due to the off axis illumination Fluorescent emission from the target fluorophore is collected through an emission filter wheel located at the top of the IVIS Lumina XR imaging chamber and then focused into the CCD camera The emission filter wheel contains eight openings Users have the ability to choose up to seven emission filters 60 mm diameter leaving one position open for bioluminescent imaging 48 IVIS Lumina XR Imaging System Manual Intensity o co o o o o te No a Width cm Figure 10 7 Typical illumination profiles for stage locations FOV of 5 7 10 and 12 cm respectively measured from the FOV center Understanding Filter Spectra The use of high quality filters is essential for obtaining good signal to background levels contrast in fluorescence measurements particularly in a high sensitivity instrume
79. tage in the imaging chamber and close the door 4 Select the Fluorescent check box 5 Make a selection from the Field of View drop down menu on the left side of the Control Panel 6 Enter the approximate 0 5 cm Subject Height height in the lower left entry box or focus manually 7 Select the Emission Filter and Excitation Filter If the Filter Lock box is checked select the excitation or emission filter of interest Select only one as the other filter will be selected automatically 8 Select High or Low from the Fluor Lamp Level drop down menu High is the recommended setting 9 Set the Exposure Time Binning and f stop Fluorescence is generally brighter than bioluminescence so the exposure time is shorter and f stop higher smaller lens opening Typical fluorescent image camera settings might be 10 sec exposure time Binning small and f 2 10 Click Acquire After the exposure is completed the overlay image is displayed NOTE During the fluorescent image acquisition the Acquire button becomes a Stop button which can be used to terminate the exposure if necessary 11 To save the data select Living Image Save Living Image Data from the main menu bar This completes the data acquisition To obtain additional images repeat the process beginning with step 3 The Living Image image window that displays the fluorescent image includes annotations specific to fluorescence including emission filter ex
80. te 8948 XPP 1 Plate Positioner 8993 XPM 2 Bioluminescent Phantom Mouse 21365 XFM 2 Fluorescent Phantom Mouse 8999 XAF 8 Anesthesia System Filters 9000 XFM 1 Low Fluorescence Mat set of 10 9001 XNC 2 Mouse Nose Cones set of 10 9006 XRS 10 Rubber Stoppers for Anesthesia Manifold 7740 XFS 20 Flat Panel Monitor 9207 XWS 260 Workbench 9208 XWS 248 Workbench 119212 XWS 272 Workbench 123280 Enhanced Excitation Filter Wheel 123325 Emission Filter Wheel Set Low Range 123326 Emission Filter Wheel Set Mid Range 126138 Emission Filter Wheel set Mid High Range 123327 Emission Filter Wheel Set High Range 123495 Optical Lens Cleaner 126291 Wipe Lint Free 50 pk 121313 FOV Targeting Mat each 3 3 Sa SB SB Sy SB By Sy SB SB SB BS SBS SB So 62 IVIS Lumina XR Imaging System Manual This page intentionally blank Y Caliper ifeSciences Appendix C Emission Filter Wheel Replacement Instructions Removing the Filter Wheel 0 tonada aea 63 Replacing the Filter Wheel s lt lt eo 65 Installing the Calibration Data 2 ee 65 This appendix explains how to replace the emission filter wheel on the following systems e IVIS Lumina e IVIS Lumina XR e IVIS Kinetic C 1 Removing the Filter Wheel 1 Move the stage to Position C 2 Remove the two rear retaining screws located in front of the rear L
81. terlock system This component is a copper plug attached to but insulated from the door When the door is open this plug removes part of the electrical circuit powering the X ray source If the copper plug becomes damaged in anyway do not operate the X ray function of the system and contact Caliper technical support Never try to defeat its function by defeating its purpose or modifying the mating safety interlock This safety interlock should be visually inspected daily for signs of malfunction WARNING Do not try to open door when any status light is red either the X Ray ON or the green red instrument status light located at the lower right hand corner of the instrument If the door handle is actuated when the status light is red the acquisition is immediately discontinued and the X ray source will need to be re armed The solenoid door lock will prevent physically opening the door until it has been unlocked in the Living Image software Dr Y Caliper Chapter 7 Operating the IVIS Lumina XR 31 Stage Curtain A stage curtain is attached to the IVIS imaging chamber platform and covers the empty space beneath the platform Figure 7 5 The stage curtain serves as a reminder to not place anything below the platform The curtain attaches to the platform by a bar that is held in place by clips at two locations If it is necessary to access this area the curtain can be easily removed and replaced To release the rod and curtain from
82. the clips pull the stage rod slightly forward Do not allow the curtain to retract around the roller To reattach the rod push the rod back into the clips Platform Clip Figure 7 5 Stage curtain 7 5 Imaging Basics Black Paper The imaging platform is a black anodized aluminum shelf with a special radiolucent insert To protect this surface and to minimize the need to clean it Caliper recommends that you image on a high quality black paper Caliper has surveyed many types of paper and recommends Swarthmore Artagain Black part no 445 109 size 8 5 inch x 11 inch This paper prevents illumination reflections and helps keep the stage clean Caliper also recommends not using the FOV mat supplied as an accessory to the Lumina XR during imaging in the X ray mode where high resolution is desired The FOV mat material permits more X ray scatter Some compromise may be necessary however if the X ray acquisition is coupled with fluorescence imaging In this case the low auto fluorescence image produced with the FOV mat material may be preferred to the X ray image with scattering Centering a Subject It is recommended that you confirm that the subject is centered on the stage before acquiring an image Manual Focus adjusts the stage position to yield the best possible focus but can also be used as a centering tool 1 Place the subject on the center of the imaging stage and close the imaging chamber door 2 In the IVIS System Co
83. the workstation from as low as comfortably possible Caliper advises having two people present during any major move to minimize the risk of damage to the imaging system If the IVIS Lumina XR is damaged during movement Caliper cannot be held responsible and any warranty will be voided 2 When you are finished moving the workstation lock the wheels If you moved the imaging system to a new location verify that the power outlets meet the power requirements for the system For more details on equipment power requirements see Chapter 6 page 19 A 3 Starting the Imaging System 1 Plug the devices into the wall sockets in the new location 2 Turn on the power surge protection devices 3 Turn on the computer and monitor 4 Turn on the VIS Lumina XR imaging chamber the power switch is located on the back of the unit and verify that the other components such as the camera power supply X ray controller both on the back of the unit and fluorescence lamp are also turned to the On position 5 After the desktop screen is displayed start the Living Image software 6 Enter a User ID up to three letters when prompted then click Done 7 To initialize the system click Initialize VIS System in the System Control panel Figure A 2 ifeSciences Dx Y Cali er Chapter A Moving the IVIS Lumina XR With the XWS 260 Workstation WJ IYIS Acquisition Control Panel IVIS Acquisition Control Panel Imaging Mode Exposure Time Bi
84. trol of the key is typically managed by a Master Key person The switch is designed so that the key cannot be removed except in the OFF position When the authorized user is finished using the instrument the key is removed from the switch Two keys are provided with the instrument and it is a good practice to archive the spare key If the keys are lost contact Caliper technical support 6 6 Imaging Chamber The imaging chamber Figure 6 4 is a highly specialized device consisting of the imaging chamber housing a heated moveable platform an auto focusing lens system with f stop control a filter wheel and sample illumination LEDs All adjustable components are motorized and computer controlled including the scintillation module The imaging chamber is light tight so that no light penetrates from the outside The interior of the imaging chamber is constructed from materials that are non phosphorescent and non fluorescent to prevent internal light contamination that could compromise sample measurements In addition to being light tight the imaging chamber is shielded to prevent X ray radiation from escaping WARNING Under no circumstances should you attempt to make any mechanical modifications to the imaging chamber with the exception of changing the emission filter wheel 24 IVIS Lumina XR Imaging System Manual Figure 6 4 VIS Lumina XR imaging chamber Imaging Chamber Features e Custom zero background imaging chamber e E

System Manual

Contents

Download Pdf Manuals

Related Search

Related Contents