SPEKOL 2000 UV VIS Spectrophotometer
Contents
1. Lamp change not available for SPEKOL 1300 From this list box select the lamp to be used for sample measurement or the wavelength at which the lamp shall be changed 7 4 SPEKOL Mode tab On this tab choose the desired measuring mode The Meas mode list box provides the following options e Scan Mode e Time Scan e Wavelengths Depending on the selected measurement mode the appearance of the bottom part of the Mode tab changes and shows only those options textboxes list boxes and buttons that are necessary for this mode Manual SPEKOL 2000 Issue 01 2008 21 Analytik Jena AG Measurement parameters for spectrophotometers of SPEKOL series SPEKOL Mode tab 7141 Measuring mode Scan Mode In Scan Mode the grating drive is continuously moving on during the measurement Settings Device Mode Meas mode 42 72 Scan Mode Range nm 150 1100 Delta lambda nm Ko Fig 7 4 Mode tab Activated Scan Mode Parameter Description Range nm Wavelength range of the scan Setting range 190 nm 1100 nm Delta lambda nm Data point interval step size in the selected spectral range Setting range 0 1 10 nm 7 4 2 Measuring mode Time Scan In this mode you may measure a sample repeatedly at a fixed preselected wavelength Settings Device Mode Meas mode aki Time Scan Wavelength nm 500 0 Waiting time Isl 0 Meas time 60 fo fo2. Photometric accuracy lt 0 01Aat1A Long term stability at 500 nm lt 0 004 A h Toluene hexan resolution gt 1 6 Line voltage 230 VAC or 115 VAC switchable Line frequency 50 60 Hz Fuses 2x T 4 AH Line fuses Temperature range 15 C 35 C Humidity lt 85 Weight 22 kg Dimensions W x H x D 410 x 275 x 656 mm Power consumption 200 VA Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 Technical data 8 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Connectors and display elements 4 Connectors and display elements OO SPEKOL 2000 analytikjena L 1 Lid of sample compartment 2 Lid for extended sample compartment 3 Status indicator LEDs Fig 1 SPEKOL 2000 overall view il 1 RS 232 port PC Fig 2 SPEKOL 2000 PC terminals on left hand side panel Manual SPEKOL 2000 Issue 01 2008 9 Analytik Jena AG Connectors and display elements 1 Ventilation slots Nameplate 3 Line power inlet jack Fan 1 Selector switch to convert to 110 V or 220 V supply level 2 Fuse holder 3 Line power switch Fig 4 SPEKOL 2000 right hand panel terminals Sample compartment Reference 1 Cell holder reference 2 Cell holder sample Fig 5 SPEKOL 2000 sample compartment Both the measuring beam and the reference beam enter the sample compartment from the back Two windows are provided to protect th
3. by any solvent vapors Wipe off spilt samples in the sample compartment or on accessory units instantly with Cleaning tissue Wipe off contamination on the instrument with a soft clean cloth slightly moistened with a commercially available neutral detergent 6 3 Replacement of fuses A defective line fuse can be replaced A Danger Electric shock Use main power switch to turn electric supply to the SPEKOL 2000 off and detach line power cable from power inlet of the SPEKOL 2000 before you replace a fuse 1 Disconnect line power cable from SPEKOL 2000 2 Open fuse holder Fig 4 p 10 by turning the bayonett connector to the left Use a screwdriver for this purpose The fuse holder will slightly come up once it is disengaged 3 Take fuse holder with fuse out and replace fuse For replacement use only fuses of this type e T4AH 4 Put fuse holder back into place and lock bayonet connector Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 13 Care maintenance 8 replacement of lamps Replacement of lamps 6 4 5 Reconnect line power cable to the SPEKOL 2000 on one end and to the line power socket on the other end Replacement of lamps gt gt gt gt Danger Electric shock Use main power switch to turn electric supply to the SPEKOL 2000 off before you replace a halogen lamp Danger Hot lamps The lamps heat up strongly during operation Allow for sufficient
4. cooling down before you replace a lamp Danger Eye injury from exposure to optical radiation Do not look into a burning lamp Its radiation may damage your eyes Soiling of lamps Do not touch the glass body of a new lamp Use particular care to protect the beam outlet window of guartz glass of the deuterium lamp If there was finger contact with a glass body use a piece of clean non shedding cloth and pure alcohol to wipe any finger marks off Contamination will deteriorate the lamp s performance features Open lamphouse 1 Turn the SPEKOL 2000 off and detach its power plug 2 Remove all samples cells and unfixed parts from the sample compartment 3 Place the SPEKOL 2000 onto a soft support base turning it upside down in order to provide access to the bottom side 1 Screws 2 Lamp cover Fig 6 Lamphouse cover at the bottom of the SPEKOL 2000 4 Turn out both screws 1 and remove lamphouse cover 2 on the bottom side 14 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Care maintenance 8 replacement of lamps Replacement of lamps 5 4 Fixing screws of deuterium lamp 2 Deuterium lamp with lamp base 3 Fixing screw for halogen lamp holder 4 Connector for deuterium lamp 5 Knurled thumb screws to fix feed wires of halogen lamp Fig 7 SPEKOL 2000 lamphouse with cover removed Replacement of halogen lamp 1 2 Open lamphouse as described above Loosen knurled thumb scr
5. notes given on the labels and the information on the use storage and disposal of reagents reaction cells and the notes on the package inserts of test kits Intended use The SPEKOL 2000 including any original accessory may only be used for the applications described in this Manual The manufacturer cannot assume liability for any other application including that of individual modules or single parts This also applies to all service or repair work that is not carried out by authorized service personnel All warranty claims shall be forfeited Local safety regulations Observe local safety regulations that are relevant to SPEKOL 2000 operation e g labor safety regulations regulations for prevention of and protection against accidents References to potential risks given in this manual do not replace the relevant labor safety regulations that are to be followed Personnel The SPEKOL 2000 may only be operated by appropriately gualified and trained personnel For this reason knowledge of the information provided by this Manual is indispensable and assumed to have been obtained Emergency stop In the case of an emergency disconnect the SPEKOL 2000 and its components from line power supply by removing the power plug from the power outlet Electric shock The SPEKOL 2000 is electrically powered At several parts inside the system extremely hazardous electrical voltages are accessible To ensure Protection Cla
6. 2000 14 Fig 7 SPEKOL 2000 lamphouse with cover removed 15 Fig 8 Holder base of halogen lamp ooooo WooWoWoWoWoW WWW W W 16 Fig 7 1 Device driver dialog DOX Jaa enek 17 Fig 7 2 Measurement parameters Scanning SPEKOL Settings tab 19 Fig 7 3 Measurement parameters Scanning SPEKOL Device tab 21 Fig 7 4 Mode tab Activated Scan Mode oooooWoWoWoWoWoW kek 22 Fig 7 5 Mode tab Activated Time Scan oooWoWooWoWoW Woo o W oWo 22 Fig 7 6 Mode tab Activated Wavelengths mode ooWoWoW oo 24 Manual SPEKOL 2000 Issue 01 2008 29 Analytik Jena AG
7. KRA kk 13 6 2 Cl6aning He ana r kena anna ha AOS 13 6 3 Replacement of fuses oooooooW Woo oma 13 6 4 Replacement of lamps oooooo W W Woman 14 7 Measurement parameters for spectrophotometers of SPEKOL Sense ae aan ee NA naa 17 7 1 Measurement parameters window for scanning spectrophotometers 17 7 2 SPEKOL Settings tab met ia aaa aslam sana 19 7 3 SPEKOL Device tab ic iais sanga anang 21 7 4 SPEKOL Mode tab an Ah NA aa tee Kania 21 7 4 1 Measuring mode Scan Mode Eiuul Wo WWW Wo WWW Wo kk kk keke 22 7 4 2 Measuring mode Time SCan Wo oWoW Wo WWW 22 7 4 3 Measuring mode Wavelengths iui EEE Wo 24 8 First measurement with SPEKOL 25 9 DIS OS alll ses ST E SEAT SS RR DYE aras T TE 27 10 rablet offfigd reSs T 29 Manual SPEKOL 2000 Issue 01 2008 1 Analytik Jena AG Contents 2 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Introduction Intended use of SPEKOL 2000 1 Introduction 1 1 Intended use of SPEKOL 2000 The SPEKOL 2000 is a compact computer controlled two beam spectral photometer to measure the transmission extinction concentration and energy level of liguid samples It is designed to perform measurement within the spectral range of 190 nm to 1100 nm Its optical system ensures genuine real time operation so even variations in the absorption of a reference sample can be compensated while measurement is going on Combin
8. Mode Title Operator UE EEE Cycle Mode Display Correction None Transmittance C No Manual 1 Reference Automatic Time controlled Number Min jo Interval time isf Max 3 lsaved Fig 7 1 Device driver dialog box The dialog box contains a toolbar with labeled buttons as well as four tabs Settings Device Mode for the selection of measurement parameters Manual SPEKOL 2000 Issue 01 2008 17 Analytik Jena AG Measurement parameters for spectrophotometers of SPEKOL series Measurement parameters window for scanning spectrophotometers Toolbar functions Function Description DA OK x Cancel Confirms the selected parameters for the following measurement s and closes the device driver dialog box The measurement parameters thus become the current measurement parameters Rejects any previous changes of measurement parameters and closes the device driver dialog box The changes will not be activated for the following measurement s Open Opens a saved measurement parameter file The Open standard dialog box appears The default path for measurement parameter files is WinASPECT para Saves the current settings of measurement parameters to the currently active file Saves the current settings of measurements parameters to a new file This will bring up the Windows standard dialog box for saving files where you ca
9. ample cell in the sample beam path m Activate the menu command Measurement Measurement or click on on the toolbar The sample measurement is immediately being started The results of the measurement appear in a separate document window You can learn more about the properties of document windows in Section Properties and functions of document windows in WinASPECT manual 26 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Disposal 9 Disposal SPEKOL 2000 owners operators will be responsible for disposal of waste sample material accruing from analytical procedures Disposal must be as reguired under valid legislation and local regulations On expiry of its service life the SPEKOL 2000 including related electronic components must be disposed as electronic waste under relevant legal provisions Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 27 Disposal 28 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Table of figures 10 Table of figures Fig 1 SPEKOL 2000 overall view oooooooooooo oooooo nan 9 Fig 2 SPEKOL 2000 PC terminals on left hand side panel 9 Fig 3 SPEKOL 2000 rear side terminalS o oooooooooooo 10 Fig 4 SPEKOL 2000 right hand panel terminals 10 Fig 5 SPEKOL 2000 sample compartment oooooooooooooooooo 10 Fig 6 Lamphouse cover at the bottom of the SPEKOL
10. analytikjena SPEKOL 2000 UV VIS Spectrophotometer 4 pE Analytik Jena AG analytikjena Kundendienst Konrad Zuse Str 1 07745 Jena Phone Hotline 4 49 3641 77 7407 Fax 4 49 3641 77 7449 e mail service analytik jena de General information about Analytik Jena AG on the internet http www analytik jena de Copyrights and Trademarks Microsoft Windows are registered trademarks of Microsoft Corp Publication No 210 021 23 Edition January 2008 Technical documentation made by Analytik Jena AG This documentation describes the state of this product at the time of publishing It need not necessarily agree with future versions of the product Subject to change Copyright 2008 Analytik Jena AG Contents Contents 1 Introduction a 3 1 1 Intended use of SPEKOL 2000 Woo Woo Wo mma 3 1 2 SPEKOL 2000 warning labels ooooooooooo Woo Woo Wcom 3 1 3 Practical notes for working with this Manual 4 2 Safety note sret AAA Ea en eta 5 3 Technicaldata soo oes eos rece ect ease L aaa s 7 4 Connectors and display elements 9 5 Installation and Start Up oo oom 11 5 1 Installation Conditions eoooWooWoWoo WWW mna 11 5 2 Connecting the SPEKOL 2000 o oooooo WoWoW Woo Waah 12 6 Care maintenance amp replacement of lamps 13 6 1 Care and maintenance aaa aaa ease kaka aaa ula aka k
11. are started manually After every measurement a dialog box appears allowing you to start another sample measurement or terminate the measurement series In this mode you need not define in advance the number of measurements to be taken in the cycle Runs automatic repeat measurements of a sample In the Number textbox type the number of repeat measurements to be run Time controlled repeat measurements for reaction kinetics In the Number textbox type the number of repeat measurements to be run In the Interval time textbox enter the desired time interval between successive measurement starts within a measurement series in seconds Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 19 Measurement parameters for spectrophotometers of SPEKOL series SPEKOL Settings tab Consider that for the actual measurement a certain time is needed If the interval time turns out to be too short during the measurement the program switches over to the Automatic mode and the measurement will then be executed as fast as possible Cr Note The time controlled cycle mode is suitable for kinetic measurements over a spectral range or at several discrete wavelengths For time dependent measurements at a single wavelength only the Time Scan mode selectable on the Mode tab is more comfortable and better suitable Section Measuring mode Time Scan p 22 Display In this field select the desired ordinate unit and the ordina
12. e photometer space from dust and exposure to reagents The holder pocket for the measuring cell 2 is located in front left position and that for the reference cell in back right position 1 10 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Installation and start up Installation conditions 5 Installation and start up 5 1 Installation conditions A Caution Do not cover any of the ventilation slots Do not place any objects on the SPEKOL 2000 Do not operate the SPEKOL 2000 in rooms where there is risk of an explosion Keep aggressive vapors e g strongly corrosive acid or caustic vapors away The installation site of the SPEKOL 2000 must meet the following reguirements The place should be free of draft dust corrosive vapors as well as vibrations Do not set up the SPEKOL 2000 near electromagnetic fields e g of motors Avoid dripping or splashing water near the instrument Do not expose the device to direct sunlight or the radiation of heaters Temperature range 15 35 C Humidity up to 85 at 30 C Footprint requirements Based on its specified overall dimensions plus an extra 100 mm stripe of clearance on all sides the SPEKOL 2000 requires a minimum footprint area of 610 x 765 mm Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 11 Installation and start up Connecting the SPEKOL 2000 5 2 Connecting the SPEKOL 2000 A Observe corr
13. ect position of device Move the SPECORD only in upright position observe label on transport case Do not turn the device upside down AN Electric shock Check that the nameplate specification for operating voltage is in agreement with the actual level of line voltage supply before you connect the SPEKOL 2000 to a mains supply point Operation at any other than the specified voltage level may result in destruction of the SPEKOL 2000 Make absolutely certain that the voltage selector switch Fig 4 p 10 is set to the right mains voltage level 1 Carefully unpack shipping case contents and visually check product delivery 2 Remove all air stream obstacles below and around the product 3 Set the selector switch to the right voltage level 1 in Fig 4 p 10 4 Connect line power cable to power inlet Fig 4 p 10 then plug power connector into power socket 5 Use RS232 cable to connect the SPEKOL 2000 Fig 2 p 9 and the PC to a free COM port 6 Remove cells or other obstacles as may be necessary from the light beam path in the sample compartment 7 Use main power switch to turn power on 3 in Fig 4 p 10 8 Start your PC and install WinASPECT software if necessary using the special SPEKOL 2000 driver refer to WinASPECT manual 9 Launch WinASPECT software and select SPEKOL 2000 via Measurement Configuration Measurement 10 Select Set parameters in Measurement Then check Device tab for correct COM in
14. ed with powerful WinASPECT software the SPEKOL 2000 is particularly suited for applications in clinical biochemical chemical or environmental laboratories as well as other sectors of routine analytics research development and guality control This User Manual contains a detailed description of the SPEKOL 2000 its specifications available accessory items and useful advice on maintenance Once the photometer has been carefully unpacked you should visually check for completeness of delivery against the packing list Likewise one should perform an inspection for proper condition On noticing any kind of non conformity you are advised to immediately contact our Representative Office or our Service 1 2 SPEKOL 2000 warning labels gt es AN Warning notes at the SPEKOL 2000 must be followed The following warning labels are provided at the SPEKOL 2000 Disconnect power cable Always remember to disconnect the power plug before you open the product casing or replace a fuse Danger UV radiation Do not look into the radiation that is emitted by the UV lamp whether directly or indirectly using a mirror Exposure to lamp radiation may cause conjunctivitis Danger Hot surface Once a lamp is turned on its shield will heat up strongly To replace a lamp or remove its shield turn the lamp off and allow for sufficient time to cool down Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 3 Introductio
15. ews for fixing of halogen lamp feed wires 5 in Fig 7 and remove cables from their clamping points Unscrew fixing screw 3 in Fig 7 of halogen lamp holder then cautiously retrieve the holder from the inner space Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 15 Care maintenance 8 replacement of lamps Replacement of lamps 1 Halogen lamp 2 Bracket with springs 3 Holder Fig 8 Holder base of halogen lamp 4 Pull bracket 3 in Fig 8 from halogen lamp Remove halogen lamp from its holder base 1 in Fig 8 5 Cautiously insert the new halogen lamp into the holder base Prevent hand contact with the lamp s surface Use a non shedding cloth and alcohol to wipe off contamination or dirt Install spring back onto the halogen lamp 6 Put holder base with the new halogen lamp back into place Fix it with the screw 3 in Fig 7 7 Clamp cable ends again with the help of knurled thumb screws 5 in Fig 7 8 Close lamphouse cover 2 in Fig 6 and fix cover with the two screws 1 in Fig 6 9 Cautiously turn the SPEKOL 2000 around again Replacement of deuterium lamp 1 Open lamphouse as described above A 2 Unfix connector of deuterium lamp 4 in Fig 7 3 Unscrew fixing screw for deuterium lamp holder base 1 in Fig 7 and cautiously remove lamp from the inner space Caution Do not touch the deuterium lamp with bare fingers Use a piece of paper or non shedding cloth for replace
16. fo fs gt J Fig 7 5 Mode tab Activated Time Scan Parameter Description Wavelength nm Wavelength to be used 22 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Measurement parameters for spectrophotometers of SPEKOL series SPEKOL Mode tab Wait time s Measuring time Data points Isl Setting range 190 nm 1100 nm The wait time is the delay between the release of the measurement and the first actual measurement This time can be used as reaction time after the addition of the starting reagent for a kinetic measurement The total measuring time can be subdivided into maximally four partial periods For every partial period you can define the measuring times and the integration times individually The measuring time is the time used for one partial period It can be specified in seconds or minutes to be selected from the corresponding list box List boxes for selection of temporal data point interval This parameter indicates the time after which the next measurement is started Setting range 1 50 0 s You can also left click on the boxes and enter any desired time via the keyboard In kinetic measurements after activation of menu command Measurement Measurement or a click on 6 first the system will make the necessary device settings driving to measurement wavelength checking the reference etc The actual measurement or wait time see above begins only after you confir
17. gths with more than one decimal the software will round the wavelength up or down to one decimal 24 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG First measurement with SPEKOL 8 First measurement with SPEKOL In this section a survey spectrum of a sample is measured as an example for a first acguaintance with the operation of the SPEKOL Beginning with the switch on of the device preparation for measurement through the entry of measurement parameters to the reference and sample measurement all steps are explained in succession Which preparations are necessary for a measurement If you installed the system you have already done the following actions e SPEKOL and PC have been interconnected by the serial interface cable e Both units have been connected to a power outlet and switched on e Onthe PC WinASPECT has been loaded You have initialized the SPEKOL If you have not done these steps yet make these steps up now To initialize the device activate the menu command Measurement Initialize Device How to set the measurement parameters The possibilities of measurement parameter selection are very extensive with the SPEKOL because of the various operating modes accessories and correction modes available For a more detailed description of the individual options refer to the Section Measurement parameters for spectrophotometers of SPEKOL series p 17 m Activate menu command Measureme
18. med the program query Start time cycle OK Thus there is sufficient time left for preparing the sample such as the addition of a starting reagent Note When you select the Time Scan mode a cycle mode automatic time controlled previously selected on the Settings tab will be deactivated Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 23 Measurement parameters for spectrophotometers of SPEKOL series SPEKOL Mode tab 7 4 3 Measuring mode Wavelengths This measuring mode allows the measurement at up to ten preselected wavelengths Settings Device Mode Meas mode ESA Wavelengths 500 0 Fig 7 6 Mode tab Activated Wavelengths mode Buttons textbox Add Edit Remove Description Adding a wavelength to the list By a click on the Add button a textbox appears for the entry of another wavelength When you confirm the entry with the ENTER key the wavelength is added to the list Editing a wavelength selected on the list By a click on the Edit button a textbox appears allowing you to edit the selected wavelength When you confirm the action with the ENTER key the wavelength on the list will be corrected accordingly Deleting a selected wavelength from the list On activation of the Remove button the wavelength will be instantly deleted from the list KP Note The system accepts wavelengths with one decimal If you enter wavelen
19. ment to prevent soiling of lamp surfaces 4 5 6 7 Install the new deuterium lamp and fix it with the two screws 1 in Fig 7 Fix connector 4 in Fig 7 Close lamphouse cover 2 in Fig 6 and fix cover with both screws 1 in Fig 6 Turn the SPEKOL 2000 cautiously around into working position 16 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Measurement parameters for spectrophotometers of SPEKOL series Measurement parameters window for scanning spectrophotometers 7 Measurement parameters for spectrophotometers of SPEKOL series This Manual only contains measurement and performance settings that are valid for SPEKOL series products For a description of any other WinASPECT software function that facilitates data evaluation and data handling you should refer to the WinASPECT Software for Spectrophotometers manual 7 1 Measurement parameters window for scanning spectrophotometers In the measurement parameters window choose the settings required for the measurement You can save the settings to a parameter file This allows you to create parameter files for different analytical tasks and recall them at any time for a measurement Open the measurement parameters dialog box by activating menu command Measurement Set Parameters SPEKOL 2000 C Dokumente und Einstellungenj All Users Dokumente Y X D E LD amp OK Cancel New Open Save Save as Help Print Settings Device
20. n Practical notes for working with this Manual 1 3 Practical notes for working with this Manual DP PB 06 Throughout this Manual special warning and information symbols are used Warning Warning messages alert you to a specific procedure or practice which if not followed correctly may cause personal injury Caution Caution messages refer you to procedures which if not observed may result in damage to the eguipment Dangerous electric voltage Emission of UV radiation Disconnect power cable Note This note must be followed to obtain correct measurement results For easier navigation this Manual is organized as follows e Chapters and illustrations are numbered consecutively e Every illustration has its own caption e Working steps are numbered e Cross references to other sections are marked by an arrow e g Practical Notes for Working with this Manual p 4 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Safety notes 2 Safety notes gt gt gt gt gt For your personal safety and for a trouble free operation carefully read this chapter before starting up the SPEKOL 2000 Observe all safety notes given in this Manual and all messages and control software notes displayed on the screen In addition observe the safety notes for system components of other manufacturers e g PC printer supplied together with the device In particular you should also observe the safety
21. n choose a new name Opens the Online Help on measurement parameter settings Print Page Layout Printer Setup Preview Print Opens the dialog box for setting the page layout Section Printing files in WinASPECT manual Opens the dialog box for selecting printer specific options Opens a dialog box with a preview of the printout Starts the printout of measurement parameters 18 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Measurement parameters for spectrophotometers of SPEKOL series SPEKOL Settings tab 7 2 SPEKOL Settings tab Title Settings Device Mode Title fC _ O Operator Cycle Mode None Manual Automatic Time controlled Number 3 Interval time 5 fi Display Correction Transmittance C No i 6 Reference C Energy Min jo Max ja Fig 7 2 Measurement parameters Scanning SPEKOL Settings tab Entries in this textbox are optional In this textbox enter e g a designation for the type of measurement to be taken with this parameter file Cycle Mode Here choose single or cyclic repeat measurements The results of cyclic repeat measurements are stored together in cycle files Option None Manual Automatic Time controlled Description Runs a single measurement normal measuring mode Runs repeat measurements of a sample The individual sample measurements
22. n on the bulb affects the radiation properties of the lamps Especially protect the beam exit window of the deuterium lamp Make sure to remove the protective cap from the halogen bulb after having inserted it to avoid any risk of fire Non observance will result in damage of the lamp Heat build up Heat accumulation might result in overheating and operating faults Make sure not to cover up the ventilation slots of the SPEKOL 2000 Before you switch on the SPEKOL 2000 remove its dust cover Handling of liquids Take care to ensure that no liquids can enter the SPEKOL 2000 to avoid any damage to the device Risk of corrosion Do not set up the device in the vicinity of aggressive vapors e g strongly corrosive acid or caustic vapors The vapors might corrode the connections mechanical and optical components of the device Avoid exposing the sample compartment to strongly corrosive substances For the analysis of such samples use vapor tight stoppered cells After the measurement do not leave the samples unnecessarily long in the sample compartment of the device Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Technical data 3 Technical data Technical data SPEKOL 2000 Optical system Double beam spectrophotometer PC controlled Optical slit width 0 5 1 2 and 4 nm Wavelength range 190 1100nm Wavelength accuracy lt 1nm Photometric range 0 1A to 3A
23. nt Set Parameters and in the appearing SPEKOLxxx dialog box choose the following settings and options Option Setting Entry Settings tab Title Enter a title of the measurement optional e g survey measurement Cycle Mode None Display Absorbance Min 0 Max 3 Correction Reference Device tab Lamp change at 325 COM Port COM port of your PC to which the SPEKOL has been connected Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 25 First measurement with SPEKOL Mode tab Meas mode Scan Mode Range nm 190 0 1100 0 Delta lambda nm 2 0 Then save the selected measurement parameters m Click on the Save as button to open the Save standard dialog box The default path for parameter files is NWinASPECTN para m Enter Survey as file name and confirm the entry with Save m Exit the SPEKOLxxx device driver dialog box with OK On doing so the parameter file just created will be automatically activated and is available for the measurements to follow How to run a reference measurement m Place the reference cell in the sample beam path of the spectrophotometer On double beam spectrophotometers this is the front beam m Activate the menu command Measurement Reference or click on 6 on the toolbar The reference measurement is immediately being started How to start the sample measurement m Place the s
24. ss I protective earth connection of the device connect the power plug of the device only to a power outlet with protective earth conductor The protective effect must not be undone by the use of an extension cable without protective earth conductor Before opening the device or removing any device covers disconnect the power cable Before connecting the SPEKOL 2000 to the power outlet make sure the line voltage supplied agrees with the operating voltage specified on the rating plate at the rear panel of the device Operation of the device with any other operating voltage than that specified may result in the destruction of the device Only use fuses of the specified type Manual SPEKOL 2000 Analytik Jena AG Issue 01 2008 5 Safety notes gt gt gt B gt gt D Do not operate the SPEKOL 2000 in rooms with an explosive atmosphere UV radiation Never look into a lamp beam whether directly or indirectly e g via a mirror Failure to comply may cause conjunctivitis Turn power off for lamp replacement Hot surface The lamphouse will heat up strongly while the lamps are on Physical contact with the hot lamp house surface may cause skin burns Turn the SPEKOL 2000 off before you proceed to lamp replacement detach the power plug and wait until the lamps have cooled down sufficiently Lamp damage Do not hold a halogen or deuterium lamp in normal working state by its glass bulb Contaminatio
25. te range in which the results shall be displayed while the measurement is running m Choose the desired option e Transmission e Absorbance e Energy m Inthe Min and Max textboxes enter the desired lower and upper limits of the display range Correction In this field you can choose the type of baseline correction reference measurement Option Description No No baseline correction is carried out Reference A reference is measured before the sample This registration of reference data is performed using the same measurement parameters as in the following sample measurement In most cases distilled water or the solvent of the sample is used as reference 20 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Measurement parameters for spectrophotometers of SPEKOL series SPEKOL Device tab 73 SPEKOL Device tab On this tab choose slit width spectral resolution the interface for PC connection and the lamp change wavelength Settings Device Mode Interface Lamp change at com zl a Slit C 5 nm C inm 2nm C 4nm Fig 7 3 Measurement parameters Scanning SPEKOL Device tab COM Port From this list box choose the COM port of the PC to which the SPEKOL has been connected Slit adjustable only for SPEKOL 2000 Choose the spectral slit width optical resolution to be used for the measurement SPEKOL 2000 SPEKOL 1500 SPEKOL 1300 0 5 1 2 4 nm 4 nm 4 nm
26. terface setting 11 Trigger initialization routine via Measurement Full Initialization This function test may take some minutes to run through Keep the sample compartment closed until initialization is complete Allow for run in time of 20 minutes Wait for the SPEKOL 2000 to reach a thermally stable condition approximately 20 minutes before you trigger the first measurement job A Caution During measurement the sample compartment cover must be closed The SPEKOL 2000 is ready for operation 12 Issue 01 2008 Manual SPEKOL 2000 Analytik Jena AG Care maintenance amp replacement of lamps Care and maintenance 6 Care maintenance amp replacement of lamps 6 1 Care and maintenance The SPEKOL 2000 is largely free of maintenance To ensure your laboratory certification with device validation Analytik Jena AG offers you a service maintenance agreement User care and maintenance is limited to the following operations e Cleaning of sample compartment and product casing e Lamp replacement Replacement of line fuses You should only use spares from Analytik Jena AG You can order consumables and wear parts by phone from our Service Department In the case of malfunction of the device please contact our Service Department For the address refer to the inside cover page 6 2 Cleaning Avoid keeping filled cells unnecessarily long in the sample compartment to prevent the spectrometer from being affected
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