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1. 14 4 3 NEW EXPERIMENT WIZARD 17 4 4 TEMPERATURE GRAPHG cccceccccecccceccccececceccacecsaceceaceceaeeceeeseaeeceaees 24 4 5 TEMPERATURE RAMPING erste sese sese 25 4 6 IAE Sado DOS e DR BER UdeMM o 26 4 7 THE HELP RG REU Da ad a di a 29 5 FREQUENTLY ASKED QUESTIONS 30 6 TOMOU SERVICE opens iones sve ansioso M METRUM EI NUM PaMEE VIDE 33 6 1 BATTERY REPLACEMENT cccccecceccecccccccsccecccccecccceccecceccecceceecessessenseas 33 7 SPECIPICATIONS EN E CURES PPM QUESO 35 8 OPERATING ENVIRONMENT cccccccccccccccccccccccccccccccccccccccccccccces 35 9 DECLARATION OF CONFORMITY ccccccccccccccccccccccecccccceccccccecs 36 10 WARRANTY Id Lda dd 38 11 NO TES oct waren TT P 40 Centeo Biosciences Ltd 3 1640 User Manual 1 TG40 System 1 1 Welcome Thank you for purchasing this Centeo Biosciences product In order to get the best performance from the equipment please read this manual carefully before using the TG40 System 1 22 Introducing the TG40 The TG40 System is designed to enable temperature optimization for vapour phase diffusion protein cr2. 1640 User Manual 3 Initial Setup 3 1 Setupthe TG40 System Connect Docking i 4 DS Station to Electrical er Power Mains e Unpack the docking station NW UM Power Connection Power switch power supply and USB to Serial cable Plug the power supply into the connector on the back of the docking station Connect the power supply to mains power The power supply will automatically detect the mains voltage from 100VAC 240VAC and supply the correct output to the docking station Ensure the power switch on the rear of the TG40 docking station is in the off position Do not connect the USB to Serial adapter cable yet 3 2 Place TG40 Microplate in Docking Station Place the TG40 Microplate in the Docking Station aligning the contacts on the TG40 Microplate with the connectors on the Docking Station Using the switch on the rear of the docking station turn on the power The status lights on the front of the TG40 Microplate will illuminate and the heat exchange fans will be heard 3 3 Install the TG40 Control Centre Software The TG40 Control Centre software runs on a PC with the Windows XP Windows Vista or Windows 7 operating systems Open the CD case and remove the CD labelled TG40 Control Centre software Please note that Microsoft Windows operating system administrator access is required to install the software 10 Centeo Biosciences Ltd 1640 User Manual Insert this CD into the PC rem
3. go to TOOLS gt SETTINGS and select the COM port that the device is connected to If you do not know the COM port select the next entry in the list Repeat this procedure until the TG40 is detected Usually there are only 1 2 items in the list Centeo Settings COM Part Please select the COM Port the TGRID is connected ta COMI Polling Multiple The temperature fram the TG40 is recorded every 3 seconds The data will be plotted the graph and stored every 3 seconds times the multiple here Please note that small multiples will result in very large data files i 2 Temperature Error Threshold Please enter the number of degrees the set point temperature before the user is warned about the temperature being out of range 4 05 20 If you have tried all COM ports in the list and the TG40 is not detected make sure that the TG40 is powered on and the data cable is connected Follow the procedures in the Operation section to create temperature controlled experiments with the TG40 System Centeo Biosciences Ltd 13 1640 User Manual 4 Operation The TG40 System operation is managed through the TG40 Control Centre Software The Control Centre Software provides continuous status updates from TG40 Microplate and enables programming of temperature control operations 41 Setting Up Crystallization Experiments with the TG40 Before setting up your first experiment with the
4. TG40 please read the Overview below to understand the process requirements Overview of Crystallization Experiment with TG40 System For best results follow these steps to set up the experiment 1 Ensure that the TG40 Docking Station is connected to power and the PC 2 Define an experiment using the TG40 Control Centre Software 3 Placean Insert into each of the five 5 rows of the TG40 Microplate 4 Using a pipette or automated liquid dispensing system load reagents and proteins into the wells 5 Sealthe inserts to prevent evaporation using sealing tape or sealing sheets 6 Return the TG40 Microplate to the Docking Station Start the experiment 8 Remove TG40 from Docking Station at regular intervals to observe crystallization The battery will maintain the set temperatures for at least 30 minutes during observations Return to Docking Station after imaging to maintain charge on battery p 42 Temperature Control Operations Reagents and protein drops can be added to the TG40 either before or after starting the experiment In either case it is advantageous to set up the experiment before proceeding to the liquid handling 14 Centeo Biosciences Ltd 1540 User Manual Start the TG40 Control Centre Software Start the TG40 Control Software and Login The default login is admin and does not require a password CENTEO amp biosciences LOGIN Username Admin Password When the software starts for the
5. as Disconnected as shown below While disconnected the temperatures on the Status tab are not updated as the TG40 Microplate is no longer connected to the Docking Station 22 Centeo Biosciences Ltd 1640 User Manual End the Experiment When the experiment is concluded such as when crystals are observed the TG40 experiment must be ended Note that an experiment will run as long as power is supplied to the Docking Station and must be ended using the TG40 Control Centre Notes 1 TG40 temperature control experiment do not have a set end time They will run until the operator chooses to end them 2 Itis important to end the experiment using the Control Centre software A new experiment cannot be started if another experiment is still running in the software To end the experiment go to the top menu select Experiment and Stop This will terminate temperature control operation and the rows will quickly go to ambient temperature The TG40 Microplate must be in the Docking Station when ending the experiment 1040 Control Centre Experiment Tools Help Start Ckri 5 biosciences Expenment 1 Finished Summay TER Summary Centeo Biosciences Ltd 23 1640 User Manual 44 Temperature Graphs The Graph tab shows a plot of the measured temperatures during the experiment To view the plot click on the Graph tab after the experiment has started The time axis on the graph will auto
6. ended with no pre set end time They continue until the operator decides that the experiment successfully produced crystals or until the operator decides that no useful crystals will be obtained At either point the operator must manually terminate the experiment 32 Centeo Biosciences Ltd 1640 User Manual 6 TG40 Service Contact Centeo Biosciences directly if your TG40 System appears to require service The TG40 System only has a single user serviceable part the battery Centeo Biosciences Ltd Polaroid Building Vale of Leven Industrial Estate Dumbarton G82 3PW United Kingdom Tel 44 0 1389 750939 Fax 44 0 1389 750939 6 1 Battery Replacement Replace battery with Centeo Biosciences Part Number CB 105 only Use of another battery may present a risk of fire or explosion Replacement batteries can be obtained from Centeo Biosciences chemical burn hazard if mistreated Do not disassemble heat Caution The battery used in this device may present a fire or above 100 C 212 F or incinerate To replace the battery remove the TG40 Microplate from the Docking Station Using the correct screwdriver carefully remove the four 4 Battery Compartment Replace battery with Centeo Biosciences Part p d h d th Number CB 105 only Uu f ther batt t isk of ozidrive screws olding e Use of another battery may present a risk o T See owner s manual for safety instructions compartment door Remove the
7. first time after installation the login screen is bypassed Summary Status Screen After login you will see the Summary status screen This screen shows the current status of the 40 System including Plate connectivity Battery status Battery charge Current temperature reading of each row Check to make sure that the TG4O Microplate module registers as connected in the software before proceeding to setup and run an experiment Centeo Biosciences Ltd 15 1640 User Manual Guidelines for setting up the temperatures of the TG40 The temperatures of the rows of the TG40 must be set according to the desired operating temperatures An ambient temperature of 20 C is assumed which is the highest ambient temperature at which row 1 can be set to 4 C Here are the temperature setting guidelines Note that the limiting temperature in these cases is the set point of Row 1 the coldest row Operation between 4 C and 9 C When Row 1 is set between 4 C and 9 C the temperatures of rows 2 to 5 have to be higher than that of row 1 in addition the temperature difference between the rows has to be 4 degrees Operation between 10 C and 50 C When Row 1 is set between 10 C and 50 C then the Minimum interval between rows O C Maximum interval between rows 4 C Note that as the ambient temperature changes the temperature setting guidelines change For example when the ambient temperature is reduced to 19 C then r
8. is the white plastic module with 5 rows of 8 wells It provides the portable temperature control for protein crystallization experiments Note that the battery is factory installed Please record the serial number of the TG40 Microplate in the space provided at the end of this manual TG40 Docking Station It provides connections to the power supply and the PC interface The TG40 Docking Station keeps the TG40 Microplate battery charged and maintains connection to the TG40 software for setting up experiments The TG40 Microplate sits in the TG40 Docking Station when not being moved N around the lab to prepare experiments or observe the results Please record the serial number of the TG40 Docking Station in the space provided at the end of this manual Battery This is a special Lithium polymer battery for the TG40 Microplate The battery fits in the compartment in the bottom of the TG40 Microplate module Care must be taken when replacing the battery to align the contacts and properly secure the battery compartment The battery is factory installed in new TG40 Systems Centeo Biosciences Ltd 1640 User Manual USB to Serial Adapter This enables connection of the serial port on the back of the Docking Station to a USB port on the computer PC controlling the TG40 system Do not connect the cable to the PC until the software has been installed See initial setup for detailed instructions Power Supply The Po
9. G40 Microplate The TG40 Microplate is automatically powered on when it is in the Docking Station and the Docking Station is powered on Why do the fans in the TG40 keep running when the Docking Station is turned off The fans run continuously while the TG40 is powered on They will continue to run even when the Docking Station is powered off until the battery charge is depleted Turning off the TG40 using the Power Reset switch will turn off the fans Caution This will terminate any experiments currently running What are the volumes of the wells in the inserts The reagent large well holds a maximum of 60uL but it advisable to use two thirds of this volume 40 LL The protein small well holds 3uL Can the TG40 Microplate sit in a standard microplate holder Yes The TG40 Microplate conforms to the footprint of an SBS standard microplate 85mm W X 128mm L H Can I operate the TG40 in a cold room Yes The TG40 can be safely operated in a non condensing environment at temperatures down to 49C Operated in a sub ambient temperature environment the minimum temperature of the TG40 will be 19C Do I have to seal the TG40 Inserts Yes they must be sealed to prevent evaporation as in other vapour diffusion crystallization experiments Use standard crystallization sealing tape sealing sheets or oils Centeo Biosciences Ltd 31 1640 User Manual How do I decide when to end an experiment TG40 experiments are open
10. User Manual TG40 System 1 Anglian Business Park Royston SG8 BTW United Kingdom www centeo com info centeo com 44 1765 252011 TG40 User Manual Publication Title TG40 User Manual Publication Date September 2010 Version Number 1 1 Product Number 5200017 Copyright 2010 Centeo Biosciences Ltd Centeo Biosciences Ltd 1640 User Manual Contents 1 Co SYSTEM 4 1 1 atis IN ERROR 4 1 2 INTRODUCING THE TG4O e en enne emen meses eren ns 4 2 UNPACKING THE 40 SYSTEM cccccecccccccccccccccccccccccccccecccccces 5 2 1 IDENTIFYING COMPONENTS OF THE TG4O 6 2 2 TG40 MICROPLATE DETAILS ccccecececcccecccccceceaceceaceceaceceaceceaceceaseaes 8 3 INITIAL SETUP sas sais di a 10 3 1 SET UP THE TG4O SYSTEM ires etr terr trea aeo isa atrasa E S rk raw 10 3 2 PLACE TG40 MICROPLATE IN DOCKING 10 3 3 INSTALL THE TG40 CONTROL CENTRE SOFTWARE 10 3 4 CONNECTING THE TG40 SYSTEM TO A 11 3 5 USING THE 40 CONTROL CENTRE SOFTWARE 11 4 OPERATION 14 4 1 SETTING UP CRYSTALLIZATION EXPERIMENTS WITH THE 40 14 4 2 TEMPERATURE CONTROL
11. ays from the date of shipment Users should retain proof of delivery date Items not covered This warranty does not apply to 40 Inserts which are consumables Warranty repair In the event of failure within the warranty period Centeo Biosciences Ltd will either repair or replace the product not conforming to this warranty It is the customer s responsibility to pay for the shipment of the product under warranty to Centeo Biosystems This warranty covers shipment charges for returning the repaired or replaced product to the customer Please contact Centeo Biosciences Ltd directly for further information A Return Goods Authorization Number must be obtained from our Technical Service before returning any product for in warranty repair or replacement 38 Centeo Biosciences Ltd 1640 User Manual Update policy We may from time to time revise the performance of our products and in doing so incur no obligation to furnish any such revisions to our customers Centeo Biosciences Ltd 39 1640 User Manual 11 Notes Please record the serial numbers and purchasing information for future reference TG 40 Microplate Serial Number TG 40 Docking Station Serial Number Date Received Purchase Order Number 40 Centeo Biosciences Ltd
12. battery i Centeo Biosciences Ltd from the box and 111 Model TG40 M Serial no CBM1010003 se Input 4 0V ITT 3 5A Made in UK Centeo Biosciences Ltd 33 1640 User Manual install with the contacts facing the connectors in the compartment Replace the compartment door and carefully tighten the screws Do not over tighten Dispose of used battery promptly Keep away from children 34 Centeo Biosciences Ltd 1640 User Manual 7 Specifications Power 110 240VAC 50 60Hz 0 7A Software Requires a PC running Microsoft Windows XP Vista 7 Operating environment 49C 309C non condensing Microplate Dimensions 127 7 mm L 85 4 mm W 28 0 mm H Temperature control range 19C to 602C Temperature accuracy 19C Well pitch and position Standard SBS 96 well 9mm Portable operation 30 minutes battery power Microplate inserts 8 wells insert 60uL reagent well 3uL protein well 8 Operating Environment The TG40 System is designed to operate at lab temperatures of 49C 309C in a non condensing environment In this range it will provide temperature control from 19C 609C See section 4 for possible combination of temperatures for a given ambient temperature For optimum performance and safety the user should consider the following e Do not touch the contacts of the docking station or short any of the pins with a conducting object e Do not operate the device next to a heat source
13. ches the start time The TG40 clock is set by the clock on the controlling PC 1040 Control Centre File Experiment Tools Help Running Experiment TGRID Status 4025 0006 CBM 1010001 Channel Temperatures 1 0 C Ld Plate Connected Connected vag J Ramp Running Lud System Status biosciences Running Time Ramp Time Remaining L4 Battery Status Charging 00 07 04 n a LJ Battery Charge 100 Experiment 1 unning Summary I Graph Ramp Temperature Summary Graph General Ramps Logs General Select Ramp Parameters R i ua Increase Temperature w by 2 Logs Over 0 Hours 10 Minutes v 02 February 2010 14 22 27 E Ramp Start Date Time Duration Minutes Status 2 0 C 02 02 2010 14 22 27 10 Not Run Currently selected ramp parameters can be added to a queue of ramps by pressing the save button Ramps in the queue can be deleted by right clicking on the appropriate entry and selecting delete from the pop up menu Centeo Biosciences Ltd 25 1640 User Manual The current temperature limits based on ambient temperature apply to temperature ramping It is not possible to ramp above or below the limiting temperatures For example if Row 1 is currently at 69C then a ramp of 49C reducing the temperature of each row by 49C is not allowed as it would take Row 1 to 29C below the operating limit at ambient temperature of 209C Note Ramps cannot be started until the experiment has been run
14. e TG40 Microplate and Docking Station are illuminated and that the USB to Serial cable is properly connected Centeo Biosciences Ltd 11 1640 User Manual From the Windows Start menu select gt Centeo Biosciences gt TG40 Control Centre When the program is run for the first time after installation it will bypass the login screen and the main screen will appear 1040 Control Centre File Experiment Tools Help TG40 Status 4024 0120 CBM1010003 Ld Plate Connected Connected Lud System Status biosciences Battery Status Charging I Battery Charge M Summary General Logs Summary Current Set Point 21 8 C FORRO enin PEDIDO DOE GCSB When an experiement is running you can exit the main window and the program will continue running inside the system tray To restore the Main window right click on gt the icon in the Windows System Tray area open You can also log on or off or to shut down Log off the program from the System Tray icon Exit Do not start multiple instances of the 14 12 56 control center through the program menu During start up of the Control Centre the software will look to see if a TG40 System is connected to the PC It may take a couple of minutes to locate the TG40 System and update the status on the screen 12 Centeo Biosciences Ltd 1640 User Manual If the TG40 isn t detected after a few minutes
15. emperature 20 Set all rows to the same temperature Operate exclusively above ambient temperature 3 Operate above or below ambient temperature Row Temperatures Define Temperature for each row Temperatures for each row must be entered next The temperatures must be within the specified temperature range of 12C 602C and within the range specified by the max and min temperatures shown next to each row Note that the entire TG40 range cannot be covered in a single experiment Note that any given row can only be set to temperature between the maximum and minimum temperatures shown for that row 18 Centeo Biosciences Ltd 1640 User Manual Describe wells for the experiment Each well can have a useful description of the protein and reagents used for the experiment Use this screen to enter any desired and useful data for the wells Note that this is optional and can be skipped by clicking Finish to complete the setup New Experiment Wizard Well Contents Well Contents Please enter the description of the contents of this well M Na amp c pH 4 8 Lysozyme The New Experiment Wizard is now complete The experiment has been saved and can be started when desired Centeo Biosciences Ltd 19 1640 User Manual 1640 Control Centre tatu A024 01 BM101001 L4 Plate Connected Connected L4 System Status Ok L4 Battery Status Charging Ld Battery Charge Experiment 1 Summary General mena ND Cur
16. ensors also report the temperature which is displayed by the software when the TG4O is in the docking station Deviations from the set temperature are logged and reported as error conditions during the operation of an experiment How long does it take the TG40 to attain the programmed temperatures Normally the programmed temperatures are attained in 5 10 minutes from the start of the experiment Once the programmed temperatures are reached they will be maintained as long as the experiment continues unless a temperature ramp is programmed Why won t Row 1 the coldest row reach 42C The ambient lab temperature may be too high If the ambient temperature goes above 209C the TG40 may not be able to attain 49C Move the TG40 to a lower ambient temperature location or modify the experimental conditions Guidelines for Temperature Programming When defining the temperature of each row there are operating parameters guidelines to keep in mind 1 There is a software limit to maximum and minimum temperature of each row based on the maximum ambient temperature and the temperature of the adjacent rows 2 Allthe wells in a row must be at the same temperature 30 Centeo Biosciences Ltd 1640 User Manual f 3 Generally each row can only differ in temperature from the adjoining row s by 49C or less 4 Every row can be at the same temperature or the rows can all be set at different temperatures How do I turn on power to the T
17. esult in very large data files al Temperature Error Threshold Please enter the number of degrees the set point temperature before the user is warned about the temperature being out of range 10 05 20 B Apply Centeo Biosciences Ltd 27 1640 User Manual Update Firmware The software program that runs inside the Microplate commonly referred to as the firmware can be updated by the user We are continuously working to enhance our products and will be supplying firmware updates on a regular basis 28 Centeo Biosciences Ltd TG40 User Manual 4 7 The Help Menu TG40 Control Centre File Experiment Tools Contents CENTE 1 biosciences Updates on the web Experiment 1 Running Summary Graph Summary This manual is available as part of the software help system In addition the menu provides an easy link to our website for software and firmware updates Centeo Biosciences Ltd 29 1640 User Manual 5 Frequently Asked Questions FAQs How do I know the TG40 reaches the set temperature The TG40 has internal temperature sensors that are factory calibrated The temperature of each row is controlled by an independent digital PID proportional integral derivative controller and measures the absolute temperature of each block The wells in the COC insert are thermally coupled to the metal block when inserted These s
18. m E E that will control the TG40 System and install the Install TG40 Control Center Software software The software will Visit the Centeo Biosciences website autorun on loading but if it Exit does not click on My Computer on the desktop Net and browse to the correct di drive Operating System Hirosot Windows XP Professional 324 Service Pack 3 CENTEO 27 Run Centeo InstallManager Exe and select Install 40 Control Centeo Software to install the software Note that during installation there will be four 4 programs installed on the PC TG40 Control Centre software FTDI USB to Serial Driver Microsoft NET Framework 3 5 SP1 Microsoft SQL Server Compact 3 5 SP2 DU De Deae The NET Framework 3 5SP1 is needed for the Control Centre Software to run Please note that most Computers will have the NET Framework 3 5 SP1 already installed If the TG40 System will be used on multiple PC s the software will need to be installed on each of them When the installation is completed remove the CD from the drive Do not start the software yet 3 4 Connecting the TG40 System to a PC Connect the USB to Serial cable s USB plug to a USB connector on the PC Connect the Serial plug to the Docking Station In the icon tray Windows will indicate that it has found new hardware and that it is installing the drivers for USB to serial 3 5 Using the TG40 Control Centre software Check that the power lights on th
19. matically change as the experiment progresses Note that after starting the experiment it typically takes 5 minutes for the TG40 Microplate to reach the set temperatures TG40 Control Centre File Experiment Tools Help Running Experiment TGRID Status 4025 0006 CBM 1010001 L4 Channel Temperatures 1 0 L4 Plate Connected Connected Ramp Running Lud System Status biosciences Running Time Ramp Time Remaining L4 Battery Status Charging 00 06 39 n a Battery Charge 100 Summary Graph General Ramps Logs Temperature o 2 5 o 9 2 10 9 2 10 9 2 10 9 2 10 9 2 18 16 00 18 17 00 18 18 00 18 19 00 18 Time 9 2 10 9 2 10 0 18 21 00 18 22 00 The data displayed in the graph can be exported for further analysis to a plain text file by pressing the export button Please note that historical graphs can only be viewed when no experiment is running 24 Centeo Biosciences Ltd 1640 User Manual 45 Temperature Ramping The TG40 Control Centre Software enables ramping of temperatures up and down during an experiment During a ramp all rows will be ramped by the same amount This is to keep constant the temperature difference between the rows and prevent undesired heat transfer among the rows To program a temperature ramp set the desired temperature change the start time and date and the duration of the ramp The ramp will start when the TG40 clock rea
20. ning for at least 10 minutes in order to ensure that the programmed temperatures are attained 4 6 The Tools menu 1040 Control Centre File Experiment Manage Users Settings F N Update Firmware biosciences Experiment 1 Summary Graph Summary Ranaral Summary User accounts Each user of the TG40 Control Centre software can have their own user account To add or manage user accounts go to the top menu select Tools and Manage Users 26 Centeo Biosciences Ltd 1640 User Manual Settings There are three main user settings 1 COM port This is the COM port is the port that TG4O is connected to 2 Polling Multiple The Software will read temperatures from the Microplate every 3 seconds when it is in the docking station The user can choose how often this data is recorded and plotted on the graph Note that for experiments running over a number of weeks it is advised to choose a multiple above 10 otherwise the resulting data file will be very large 3 The software will generate a log entry if the setpoint temperatures are outside the thresholds entered in the settings Centeo Settings COM Port Please select the COM Port the TRRID ts connected to COM Folling Multiple The temperature from the 7640 is recorded every 3 seconds The data will be plotted on the graph and stored every 3 seconds times the multiple here Please note that small multiples vall r
21. ow 1 can be set to 3 C The absolute temperature range the TG40 can control is 1 C to 60 C however the entire range is not accessible in a single experiment 16 Centeo Biosciences Ltd 1640 User Manual 43 New Experiment Wizard From the File menu select New to set TG40 Control Centre up an experiment This starts the New Experiment Wizard Import Alt F4 Name the experiment Lee When the New Experiment wizard starts give the experiment a name enter any descriptive information that is useful and click Next New Experiment Wizard New Experiment Wizard Step 1 General Experiment Name Experiment 1 a Experiment Description 05 01 2010 My Experiment Define Temperature Control Operations The next screen in the New Experiment Wizard controls the temperature settings Enter the ambient temperature in the laboratory where the TG40 System will operate While the TG40 module will actively measure the ambient temperature during operation the Ambient setting is needed to define the operating range Next decide what temperature control operation to select 1 Set all channels to same temperature 2 Operate exclusive above ambient temperature 3 Operate above and below ambient temperature Centeo Biosciences Ltd 17 EM TG40 User Manual Click Next to go to the next screen New Experiment Wizard New Experiment Wizard Step 2 Temperatures Average Ambient T
22. rent Logs Set Point Summary Logs 21 7 C 20 0 Experiment Selection 21 8 C Pane DON DOADA 21 8 C URIA RUE RIR DURO 21 9 C 8 0 22 2 C 40 C Start the Temperature Control Experiment To start an experiment select the experiment in the selection pane on the left of the screen then go the top menu and select Experiment and Start Note that the Summary Tab provides complete system status indicating that the TG40 Microplate is connected the Status is OK and the battery is charging The current temperatures of each and the experimental set point temperatures for each row are shown also Note that the experiment can be started either before or after the addition of reagents and protein drops If you desire to have the TG40 rows at the set temperatures prior to adding the reservoir reagents and the protein drops start the experiment and allow time to reach the set temperatures This will take typically 5 minutes 20 Centeo Biosciences Ltd 1640 User Manual depending on the set temperatures and the ambient temperature for the TG40 rows to stabilize at the programmed temperatures Once these programmed temperatures are attained remove the TG40 Microplate from the docking station and perform the liquid handling reagent and protein dispensing tasks The battery will maintain the set temperatures for at least 30 minutes Seal the inserts and return the TG40 Microplate to the Docking S
23. such as a hot plate or radiator e Do not expose the device to intense sun light when running an experiment e Do not operate the device next to or under equipment that produces a fast moving stream of air such as air conditioning equipment or fans including computer fans When the device is set to operate at temperatures above 40 C do not touch the thermal blocks containing the wells This is the maximum range Range at any given ambient temperature will be less Centeo Biosciences Ltd 35 1640 User Manual 9 Declaration of Conformity Thermally Controlled Microplate with SBS footprint Model TG40 This product complies with the requirements of the EU Directives listed below Electromagnetic Compatibility Directive EMC 2004 108 EC Low Voltage Directive 2006 95 EC Compliance with these standards is claimed by meeting the following requirements EN61326 1 2006 EMC and 61010 1 2001 LVD Compliance Certificates and Full Reports Ref 2927TC1 2926TR1 2925TC1 2924TR1 Signed Cte i Authority Dr Philipp Steinmann Technical Director Date 02 02 2010 For Centeo Biosciences Ltd Polaroid Building Vale of Leven Industrial Estate Dumbarton G82 3PW United Kingdom Tel 44 0 1389 750939 Fax 44 0 1389 750939 36 Centeo Biosciences Ltd 1640 User Manual FCC This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to par
24. t 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at their expense Centeo Biosciences Ltd 37 1640 User Manual 10 Warranty TG40 System Product Warranty Centeo Biosciences Ltd warrant that the TG40 System is free from defects in labor and materials and shall conform to its product specifications as defined in the product documentation This warranty covers parts except those specified below and labor and applies only to equipment which has been installed and operated in accordance with the documentation supplied by us and which has been serviced only by authorized dealers or service personnel This warranty does not apply to equipment and accessories that have been modified or tampered with in any way misused or damaged by accident neglect or conditions beyond our control Warranty period TG40 Microplate and Docking Station are covered by a standard warranty that is one 1 year from the date of shipment The TG4O Battery is warranted for 90 d
25. tation to continue the experiment 1040 Control Centre File Experiment Tools Help Running Experiment TG40 Status Not Connected E N T O Ld Channel Temperatures 1 0 C Plate Connected Disconnected 122 Ramp Running System Status Unknown biosciences Running Time Ramp Time Remaining Battery Status Unknown 00 18 23 n a Battery Charge Unknown Experiment 1 Running Summary Graph General Ramps Logs Summary Current Set Point 20 0 C 20 0 C 16 0 C 16 0 C I5 CHAH ERR OURO E RT DCL 11 99 COO oc 7 9 8 0 C 4 0 C 4 0 C Alternatively the liquid handling and insert sealing procedures can be accomplished prior to starting the experiment In either case the set temperatures will be maintained as long as the experiment is running and the TG40 Microplate is in the Docking Station At any time during the experiment the TG40 Microplate may be removed from the Docking Station for observation or imaging of the wells While the TG40 Microplate is removed from the Docking Station the battery Centeo Biosciences Ltd 21 1640 User Manual provides power to the TG40 Microplate maintaining the set temperatures and recording the status in the microplate memory Typically the TG40 Microplate will operate for at least 30 minutes under battery power When removed from the Docking Station TG40 Control Centre software will show the status
26. te is a small recessed button that can be used to turn the TG40 Module on or off Use a pen to activate the button pushing against it The Microplate will automatically switch on when it is docked and the main power switch at the back of the docking station is switched on To turn off the TG40 Microplate for storage or when no experiments are being conducted make sure that the plate is either undocked or the main power switch at the back of the docking station is in the off position Then push the Power Reset Switch This switch can also be used to reset the TG40 Microplate to clear experiments that were previously loaded To reset the device push the button and keep it pressed for 3 seconds then release Centeo Biosciences Ltd 1640 User Manual Status Indicator Lights There are three indicator lights on the front of the TG40 Microplate module These lights indicate the following from left to right Solid Green Flashing Green Solid Red Flashing Red Temperature control active and temperatures in range Temperature out of range Set points not reached Temperature control inactive Centeo Biosciences Ltd Docked Battery fully charged Undocked Battery Charge gt 50 Docked Battery charging Undocked Battery Charge lt 50 Undocked Battery Charge lt 10 No Battery Running on external Power Non critical error Critical error Running on battery power
27. wer Supply looks like a standard wall transformer It provides the appropriate power to operate and charge the TGAO Microplate system The power cable connects to the back of the Docking Station See initial setup for detailed instructions Do not unplug the power supply from the mains socket while an experiment is running If the docking station needs to moved to different location while an experiment is running disconnect the plate first CD Contains the TG40 Control Centre software and communications software See initial setup for detailed instructions Inserts The TG40 Microplate uses specially designed inserts made from cyclic olefin copolymer COC for crystallization experiments These Inserts provide good thermal contact to the metal heat exchangers of the TG40 Microplate Each Insert has eight 8 wells for crystallization reagent and protein sample drop The maximum volume that the reagent large wells hold is 60uL however for protein crystallisation we recommend using two thirds of the total volume 40 uL The protein small wells hold 3uL Each experiment requires five 5 inserts Plastic Lid The plastic lid fits over the top surface of the TG40 Microplate and can be used to reduce condensation and provide additional temperature stability Centeo Biosciences Ltd 7 1640 User Manual 2 2 TG40 Microplate Details Power Reset Switch Located on the right hand side of the TG40 Micropla
28. ystallization experiments The TG40 Systems provides five 5 rows of eight 8 wells in a temperature controlled microplate The TG40 Microplate has an SBS footprint to fit into standard laboratory systems for liquid handling and automation Each row can be set to a different temperature so that eight 8 experiments can be conducted simultaneously at five 5 different temperatures The goal is to find the temperature that provides the most optimal crystals 4 Centeo Biosciences Ltd 1640 User Manual 2 Unpacking the TG40 System The TG40 system includes the TG40 Microplate TG40 Docking Station COC inserts a Plastic Cover Battery USB to Serial adapter cable power supply with mains plug and a CD with the software Unpack these items carefully and ensure that they are all present before proceeding with initial setup Note that the battery is pre installed and is not shipped separately The TG40 System is packed in recycled packaging material as far as possible designed to minimize carbon footprint Please retain the packaging until you have determined that all components were received and in good working order When the packaging is no longer needed please recycle or reuse it Centeo Biosciences Ltd 5 1640 User Manual 2 1 Identifying Components of the TG40 System Here is a listing of the components and their function Please ensure that all components are present before starting the setup TG40 Microplate This
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