Pharmacia MultiTemp III user manual
Contents
1. Set the safety temperature limit using the dial on the rear of the unit Set the setting range limit of the circulator if required by the application or any safety precautions Set the working temperature Press Pe to select the S mode 5 FG Use Ad or aU to set the working temperature Confirm with enter before the display automatically returns to the actual working temperature Set the temperature correction value if required O amersham pharmacia biotech Printed in Sweden by TK i Uppsala Sept 19982. aaa reset 3 Press for start The version number of the software is briefly shown on the display The pump motor and compressor start up liquid starts to circulate and the heater will be activated if the set temperature is greater than the actual temperature Add more liquid if necessary until the level is 2 cm below the top plate and proceed as described under Section 4 2 Setting the temperature limit Resetting mode 8 to OFF Change to mode 8 OFF with the following procedure 1 Press The display flashes f 2 Press w mode 3 Press select mode 8 simultaneously with the main switch PAS Press to select OFF 5 Confirm by pressing when the display is flas hing 6 Wait until the display shows the actual temperature 7 Switch off the unit using the mains switch The operation mode is now changed to OFF To start MultiTemp III in 8 mode OFF follow the instruc tions described in Section 4 1 Start up 21 8 Technical specifiations 22 8 Technical specifications Technical specifications ambient temperature 20 C Working temperature range Temperature control constancy at 20 C Range of temperature limit switch Heater capacity 230V 115V Cooling capacity 115V at20 C at 5 C Cooling capacity 230V at 20 C at 5 C Compressor coolant Bath volume Bath opening depth Pump capacity pres
3. dent of the control temperature sensor that regulates the set working temperature The temperature cut off limit is set on the dial at the rear of the circulator This has a range of 20 to 95 C You should select a cut off limit that is 5 to 10 C above the set working temperature If the temperature rises above this limit the general cut off is activated and the following error message is displayed AL DC After the bath liquid has cooled down to below the cut off limit the following code is fa Pec displayed The built in temperature limit switch also senses low levels of coolant If the liquid level falls and exposes the heating ele ment the temperature will rise above the temperature limit cut off level and the general cut off will be activated Top up with coolant to remedy this situation The same two error messages shown above will be displayed Restart the unit by pressing Regular topping up with the liquid coolant is recommended See Section 4 1 Start up 18 7 Detailed Description Accurate Setting of Temperature Limit If a more accurate temperature limit is required for example 50 C follow the procedure below 1 Set the temperature limit switch to its highest value 2 Set the working temperature to 50 C according to Section 4 4 3 Run the unit and wait until it reaches exactly 50 C Turn the temperature limit dial very slowly anticlock
4. III must be manually re started using reset after power failure See Section 7 5 Manual restart in mode 8 enter Enters a set value if pressed before the display returns to actual temperature value The display changes from flas hing to constant to confirm the enter operation reset After the safety circuit has shut down MultiTemp III and the error has been eliminated press fest to re start the unit Warning After a power interruption the circulator restarts automatically Ensure that this cannot cause a dangerous situation Note If you need to start MultiTemp II manually after power failure by pressing reset proceed directly to Section 7 5 Operation mode 8 17 7 Detailed Description 7 4 Description General cut off of safety The heater the compressor and the circulation pump can be features switched off by a relay built into the unit This general cut off will be activated if a malfunction is detected by the safety sensors When the general cut off switch is activated the circulator can only be restarted after the cause of the indicated fault has been eliminated The display will show when this has occur red The circulator can then be re started by pressing the Reset key Protection against over heating A built in temperature limit switch protects against uncon trolled heating of the bath liquid by activating the general cut off if this limit is exceeded This safety device is indepen
5. by service per sonnel Restart after power failure MultiTemp III automatically restarts after a power failure Make sure that this automatic restart will not cause a dange rous situation to arise The unit can be restarted manually instead by pressing P see Section 7 5 MultiTemp III is factory set to normal mode Normal mode means that the unit automatically re starts after power failu re As most modern electrophoresis power supplies automati cally restart after short power failures we strongly recom mend that you leave MultiTemp III set in its normal mode However if automatic re start is inconvenient the unit can be restarted manually by setting Operation mode 8 to ON follow the procedure below Setting mode 8 to ON mode 1 Press simultaneously with the mains switch Then mode again until mode 8 is selected on 2 Press J iii to select ON instead of OFF OFF is factory set 3 Confirm by pressing hing press ener when the display is flas 4 Wait until the display shows the actual temperature 5 Switch off the unit using the mains switch The operation mode is now changed to ON 7 Detailed Description To start MultiTemp III in 8 mode ON follow the instructions below 1 Check that all tubings are firmly connected and secured with hose clamps 2 Switch the power on with the mains switch The display flashes gl
6. wise temperature down until the safety circuit is trigge red and the display indicates a malfunction 5 Change the working temperature setting to any value lower than 50 C 6 Wait until the bath liquid has cooled down to below 50 C 7 Press the reset key The circulator can now be used for temperatures up to 50 C As soon as 50 C is reached the unit switches off Protecting the pump motor When the temperature in the winding of the pump motor reaches a certain level the general cut off will be activated This protection prevents the motor from overheating The following error message will be displayed Al P After the winding of the pump has cooled down the following code A DOO is displayed Check the pump and wait a few minutes until the motor tem perature has dropped further before restarting the unit If this fault happens frequently contact service personnel Sensor check The temperature sensor is permanently monitored The gene ral cut off is activated if sensor breakage or current leakage is detected This fault is indicated by lA L F i 19 7 Detailed Description 7 5 Manual 20 restart in operation mode 8 Note Although this feature activates the general cut off it is not a dedicated safety function of the type listed above When the error message shown above is dis played the sensor must be exchanged
7. 2 Siria 4 2 3 Safety features iaia 5 De Instalati 5 oe sasecdenesscseGeceatacabeveuectatecunsaenSaueseiaccaueleatedesss 6 3 1 Unpacking and installation 6 3 2 Tubing COMMECHOUS sects csncsssccesenscreaseerdacsoieneesdcennees 7 3 3 Recommended bath liquida 7 3 4 Filling and draining the bath 8 3 5 Mains CONNECTION urina 8 LO LL PRRPRRTO PRA RR RS 9 A SS CAME up 9 4 2 Setting the temperature limit 0s0csseseeerees 10 4 3 Temperature setting limit scssssccccsssseeecees 10 4 4 Temperature setting iridata 11 4 5 Temperature correction value 11 5 Trouble shooting anne 13 I EmOr MESSAGES argani 13 6 Maintenance ivi icvccenoacdedacceaueceneeas caved cane caceucceauncareedatencceanas 14 Goll G neral issscicines eadeencatesssdabiciacasasectdaveanedameadasanceasacs 14 G2 O A E EN Rae idea 14 7s Detailed Descripti ti sesisissisrcrnisrerssntississrscnrssiiaiss 16 7 1 General operating prmeiple 10 16 7 2 Front and rear panels ssssessseserssssereesssresesecerssseee 16 To Gotto Pale aironi 16 7 4 Description of safety features 18 7 5 Manual restart in operation mode 8 20 8 Technical specification rina 22 9 Ordering officinale 23 Short instructions Back page 1 Introduction and 2 Safety information 2 1 Safety precau tions 1 Introduction MultiTemp III circulates constant temperature
8. IEC 536 all conductive parts that might become accessible are connected to earth e Protection IP 32 according to IEC 529 EN 60529 protec tion against accidentally touching accessible live parts by penetration of particles with a thickness or a diameter of more than 2 5 mm and protection against dropping water onto the unit inclined at max 15 degree angle This product meets the following EG directives e 72 23 EEC through the harmonised standard EN 61010 1 1993 A1 1992 Safety requirements for electrical equipment for measurement control and labora tory use Part 1 General requirements Part 2 Requirements for laboratory Equipment for hea ting of material Note The operator has to apply the safety precautions listed in section 2 1 above e 89 336 EEC through the standards EN 50081 Part 1 03 93 Electromagnetic Compatibility emission standard EN 50082 Part 1 03 93 Electromagnetic Compatibility Generic immunity standard The CE symbol and corresponding declaration of confir mity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked Amersham Pharmacia Biotech instruments or connected to other products recommended or descri bed in this manual and is used in the same state as it was delivered from Amersham Pharmacia Biotech except for alterations described in this manual 1 Introduction and 2 Safety information 2 3 Safety features Genera
9. Multi Temp III 18 1106 33 Edition AE User Manual amersham pharmacia biotech Important user information Reading this entire manual is necessary for full under standing and safe use of this product Should you have any comments on this manual we will be pleased to receive them at Amersham Pharmacia Biotech AB SE 75184 Uppsala Sweden Amersham Pharmacia Biotech reserves the right to make changes in the speci fications without prior notice Amersham Pharmacia Biotech AB 1998 All rights reserved Trademarks MultiTemp and Multiphor are trade marks of Amersham Pharmacia Biotech Limited or its subsidiaries KLEA 134a is a trademark of ICI Amersham is a trademark of Nycomed Amersham plc Pharmacia and Drop Design are trade marks of Pharmacia amp Upjohn Inc Terms and Conditions of Sale All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Pharmacia Biotech group which supp lies them A copy of these terms and conditions is available on request Office Addresses Amersham Pharmacia Biotech AB SE 751 84 Uppsala Sweden Amersham Pharmacia Biotech UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Pharmacia Biotech Inc 800 Centennial Avenue P O Box 1327 Piscataway N J 08855 1327 USA Contents 1 Introductioni ssaa 3 2 Safety informati ana 3 Zipoli 3 2
10. Tempera ture correction value 2 Use a or J to set the maximum allowed set tempe rature The factory pre set value is 90 0 C enter 3 Confirm with al temperature value before the display returns to the actu enter Note The new value is not saved until has been pres sed m LI uu 1 Press sa to select the S mode 5 2 Use or J to set the working temperature 3 Confirm with before the display automatically returns to the actual temperature value enter Note The new set value is not saved until has been pressed The display continuously indicates the actual temperature in the bath at the position of the sensor This temperature does not correspond directly to the temperature in the externally connected equipment Under most circumstances this tempe rature difference is very small To adjust the display value to reflect the temperature in the external system proceed as fol lows 1 Measure the temperature in the external system 2 Calculate the temperature difference between this value and the value displayed Taiff Tdisp Tmeas Taiff is the correction value c that has to be entered mode 3 Press to select the C mode alg 4 Use 1 or to set the correction value with its or sign The range is 25 0 to 2 50 C The factory pre set value is 0 0 11 4 Operation enter 5 Confirm with before the display re
11. eating and maintains the liquid temperature constant at any pre set level See Figure 1 for a description of the front and rear panels with connection points for cooling liquid and power Cin LI ASA The five digit numeric display shows either the actual opera ting temperature error messages or the set temperature After selecting mode or setting temperature the display automati cally switches back to the actual temperature after approxi mately 10 seconds In the actual temperature mode a constantly lit dot in the left digit position indicates that the heater is working to reach the set temperature A flashing dot indicates that the liquid in the bath has reached this working temperature COOLING The control light is lit only when the compressor is in operation 7 Detailed Description Mains Switch Starts the cooling compressor and the pump motor that circulates the temperature controlled liquid Activates the heater if the temperature set is greater than the actual temperature LA Change the temperature value Values are changed in an accelerating manner when either key is held down mode Changes between showing the actual temperature and the various program modes Actual temperature mode shows the actual temperature in the bath S mode sets the working temperature c mode sets temperature correction value H mode sets the maximum allowed set temperature 8 mode in 8 mode on MultiTemp
12. ked display Undefined cause Press both arrow keys and the main switch simultaneously to get 7 fai E in the display LIO dl Switch off the unit and restart Note The unit is now changed to the factory set parameters 13 6 Maintenance 6 1 General 14 6 Maintenance Warning Always disconnect MultiTemp III from the mains Pull out the mains plug before maintenance and or service Service should be carried out by autho rised personnel only To maintain efficient operation of the compressor cooling unit have the air grid and liquifier heat exchanger checked and cleaned by service personnel two to four times a year depending on the amount of dust in the air Always ensure the liquid bath has sufficient circulating liqu id The cooling coil in the bath and the bath vessel itself should be periodically checked and any deposits removed Metal particles and acidic or alkaline substances should be removed quickly as they could harm the stainless steel material and cause corrosion After some time the stainless steel surface and the housing may show spots and become tarnished Clean them with stainless steel liquid cleaners normally used in the kitchen Do not use scouring pads or powder Disposal MultiTemp III will provide many years of efficient operation When its working life is over however please remember that the unit contains coolant R 134a which despite its environ mental frie
13. l Add more liquid until the level is once again 2 cm below the top plate If the safety circuit is triggered please top up with more liqu id and restart the unit using fest Repeat this action if necessary Note Check the liquid level after the unit has been in use for a while When MultiTemp III is switched on the compressor starts and the heater will be activated if the set temperature is grea ter then the actual temperature A constantly lit dot in the left digit position 9 in Fig 2 indicates that the heater is working to reach the set temperature A flashing dot indica tes that the bath has reached the set temperature Note Setting a temperature limit is important for the safe operation of the unit It does not set the working temperature Set the temperature limit using the dial on the rear of the unit The scale shows approximate temperatures Set the dial to a value 5 10 C 8077 TINO i s 60 40 above the desired working temperature If a more accurate temperature limit is required the procedu re described in Section 7 4 should be followed The range of temperatures that can be set can be limited if required by the application or for other reasons Caution This is a setting limitation not a primary safety measure The temperature limit protection must be set separately see Section 4 2 mode E 7 m 1 Press to select the H mode HI 19010 4 Operation 4 4 Tempera ture setting 4 5
14. l cut off The heater the compressor and the circulation pump can be switched off by a relay built into the unit This general cut off will be activated if a malfunction is detected by the safety sensors See 7 4 Description of Safety Features Protection against over heating A built in temperature limit switch protects against uncon trolled heating of the bath liquid by activating the general cut off if this limit is exceeded The built in temperature limit switch also senses low levels of coolant See 7 4 Description of Safety Features Pump motor protection When the temperature in the winding of the pump motor reaches a certain level the general cut off will be activated preventing the motor from overheating See 7 4 Description of Safety Features Sensor check The temperature sensor is permanently monitored The gene ral cut off is activated if sensor breakage or current leakage is detected See 7 4 Description of Safety Features Restart after power failure MultiTemp III automatically restarts after a power failure See 7 4 Description of Safety Features 3 Installation 3 1 Unpacking and installation 3 Installation 1 Check the contents against the packing list supplied Inspect or any damage that may have occurred during transit Report any damage immediately to your local Pharmacia Biotech representative and to the transport company concerned 2 Place MultiTemp III in its working location en
15. liquid to external electrophoresis equipment The liquid temperature range is 10 to 90 C The unit contains KLEA 134a an ozone friendly HFC type coolant MultiTemp III both supplies coolant for high voltage techni ques and provides temperature control at higher temperature e g 60 C when performing DNA sequencing When used with equipment such as the Amersham Pharmacia Biotech Multiphor II electrophoresis unit two units can be connec ted to MultiTemp III at the same time MultiTemp III can also be used as a stand alone constant temperature water bath 2 Safety information Read this entire manual before using MultiTemp III 1 This instrument is designed for indoor use only 2 The unit must be connected to a grounded mains socket 3 Ensure that the vents at the sides of the instrument are not covered 4 Do not use in a dusty atmosphere or close to spraying water 5 Set the temperature limit cut off correctly 6 Use only water or water with ethylene glycol in the bath 7 Do not use the circulator beyond its specifications or for other than its intended use 1 Introduction and 2 Safety information 2 2 Safety standards 8 Do not touch the stainless steel bath cover and tubings when the unit is used at a high working temperature Under these conditions the surface temperature can rise up to approximately 70 C MultiTemp III is designed in accordance with e Protection class 1 according to
16. ndliness may only be discarded by authorised per sonnel 6 Maintenance 6 2 Fuses The circuit breaker thermal fuses for MultiTemp III are loca ted on the rear of the unit Note If the circuit breaker has been triggered there may be some delay before it can be released Please push the circuit breaker button only do not pull PUSH Ratings for the circuit breaker for 198 to 253 V version two circuit breakers 2 x 8 Amps for 95 to 132 V version one circuit breaker 1 x 13 Amps Fuses for the electronics located inside the unit must be repla ced by authorised personnel only for 198 to 253 V version fuse 1 x 40 mAmps T for 95 to 132 V version fuse 1 x 63 mAmps T 15 7 Detailed Description 7 1 General operating principle 7 2 Front and rear panels 7 3 Control 16 panel 7 Detailed Description MultiTemp III regulates temperature by balancing the effects of constant cooling and controlled pulses of heating Constant cooling is provided by a built in unit that includes a compressor an air cooled liquifier and an evaporator a coo ling coil as heat exchanger located in the chamber This coo ling unit operates at temperatures up to 68 0 C Beyond this temperature the compressor automatically switches off Heating is provided by pulses of power at a frequency esta blished by the set working temperature This type of control achieves an optimal balance between cooling and h
17. ning the screw on the front of the unit see Fig 1 Warning Do not drain hot water Use protective gloves and clothing when draining the bath liquid Compare the local mains voltage and frequency with the spe cifications written on the label on the rear of the unit before connecting the unit to a grounded mains socket Deviations of voltage and frequency must not be more than those speci fied in this manual 95 to 132 V 60 Hz 95 to 125 V 50 Hz 198 to 253 V 50 Hz Connect the unit to a grounded mains socket only The socket must be rated as suitable for the total power consumption of the unit 1 200 W 4 Operation 4 Operation 4 1 Start up COOLING Fig 2 Front panel of MultiTemp III 1 Mode selector 6 Mains switch 2 Set value down 7 Control lamp cooling on off 3 Set value up 8 Display 4 Enter 9 Heater control sign 5 Reset 10 Display Before switching on confirm that the outlet and inlet nozzles are either firmly connected to external equipment or to each other and that the tubing is secured with hose clamps Switch the power on at the mains switch The version number of the software is briefly shown on the display lt lt n Fad After starting the unit the pump will circulate the liquid to 4 Operation 4 2 Setting the temperature limit 4 3 Tempera 10 ture setting limit the external system and the level in the bath will fal
18. section Condensation on the cooling plate of Multiphor II during sample application can be avoided by shutting off the circula tion to the cooling plate with a three way valve See 3 way Valve Set in the Ordering Information section Note Do not touch or remove tubing when the unit is in operation Stay alert to the dangers of hot water The surface temperature of the tubing can reach 70 C at high working temperatures Use distilled water in the temperature range of 5 to 90 C A mixture of distilled water and ethylene glycol in a concentra tion up to 20 is suitable for lower ranges 10 to 40 C Warning Ethylene glycol is poisonous Handle with care and follow recommended safety procedures Warning Methanol and ethanol are not recommended because of their low flash points Both liquids are unsa fe to use Remove the plastic lid and fill the bath with liquid The dis tance between the surface of the liquid and the stainless steel top plate should be about 2 cm If it is more than 4 cm a sensor will activate the temperature limit switch and cause cut off 3 Installation 3 5 Mains connection To drain the bath switch off the mains power and remove the outlet tubing from the external equipment and let the liquid pour into a large vessel using hydrostatic pressure The bath contains 3 litres of liquid plus the volume in tubing and connected equipment The last millilitres can be removed by ope
19. sure max Pump capacity flow rate max Noise level at 1 m in front of the unit Mains requirements Power consumption Ambient operating humidity 80 at 31 C maximum 50 Ambient operating temperature Ambient operating pressure Dimensions H x W x D Weight 10 to 90 C lt 0 1 C 20 to 95 C 800 W 800 W 240 W 200 W 265 W 215W R134a 3 litres 130x100 100 mm 0 32 bar 12 l min lt 63 dB A 95 to 132 V 60 Hz 95 to 125 V 50 Hz 198 to 253 V 50 Hz 1 200 W Relative humidity maximum at 40 C 4 to 40 C Maximum altitude of 2 000 m 480 x 220 x 420 mm 28 kg 9 Ordering information 9 Ordering information Designation Code No MultiTemp III 100 120 V 18 1102 77 MultiTemp III 220 240 V 18 1102 78 Accessories Item Quantity Code No Cooling Tubing 8 12 mm silicone 4m 80 1106 56 Tubing Connector Set female and male 4 18 1104 26 Hose clamps 10 18 1104 27 Insulation for cooling tubing 14 27 mm 8m 80 1116 11 3 way Valve Set 1 18 1106 39 23 Short instructions Note The following short instructions are intended as a guide to users who are fully familiar with safety procedures and operating instructions described earlier in this manual Check to make sure that all tubings are firmly connected and secured with hose clamps Check the liquid level and fill the bath if needed the level should be 2 4 cm below the lid Switch the power on at the mains switch
20. suring ade quate space for ventilation of air through the grids on the sides air outlet and rear air inlet of the unit 3 Allow MultiTemp III to stand in an upright position for 24 hours before use Compressor lubricant may have run into the liquifier and evaporator if the unit has not been transported in an upright position 2 3 4 Fig 1 Front and rear view of MultiTemp III 6 Circuit breaker 1 Control Panel 220 V two thermal fuses 2 Temperature limit dial 115 V one thermal fuses 3 Outlet l 7 Bath Drain I as i 8 Handles front and rear 4 Inlet nozzle 9 Covet lid 5 Mains cable 10 Air ventilation 3 Installation 3 2 Tubing connec tions 3 3 Recom mended bath liquids 3 4 Filling and draining the bath Silicone tubing inner diameter 8 mm can be used over the entire working temperature range of the unit PVC tubing can only be used in the temperature range of 10 to 60 C 1 Connect the inlet and outlet tubing to the nozzles on the rear of the circulator Fig 1 2 Connect the other ends to the electrophoresis unit The nozzles have an outer diameter of 9 5 mm To ensure safe tubing connections secure all tubing with hose clamps When MultiTemp III is used as a water bath the nozzles should be connected to each other Condensation of water on cold tubing can be avoided by covering it with insulating tubing See Insulation for cooling tubing in the Ordering Information
21. turns to the actual temperature value i fenter Note The new value is not saved until has been pres sed 12 5 Trouble shooting 5 Trouble shooting 5 1 Error messages Message on display Cause Remedy g I ar The bath temperature has exceeded Pour in more liquid SIL the safety temperature limit Too low water level Pour in more liquid fa P The winding of the pump motor is too hot Check pump and wait a few minutes until the Vie motor temperature has dropped If this fault occurs often return the unit for service ms C The temperature sensor is broken The sensor must be exchanged by service A L U personnel n alalar The bath liquid has cooled down below Check the water level in the bath and L the temperature limit adjust if necessary Restart the unit by pressing reset g g Gln n The winding of the pump has cooled down Check pump and wait a few minutes until the U below the alarm level motor temperature has dropped If this fault occurs often return the unit for service Restart the unit by pressing reset ol Doo F The temperature sensor is Restart the unit by pressing reset functioning again This error may require service personnel G nono An undefined fault has occurred However before contacting service personnel top up the bath with some water This can solve the problem Loc
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