BioDoc-It Imaging System - Cole
Contents
1. UVP Focus Target The UVP Focus Target fluoresces when placed on a transilluminator or when exposed to overhead UV The target provides sharp fluorescent images to aid in adjusting the focus of the camera Removable USB Stick The removable USB stick included with the system has 1GB memory minimum and connects to the system to allow for saving and transferring of images UVP Focus Target BioDoc It Imaging Systems 6 Optional Equipment Refer to the Replacement Parts and Accessories section of this manual for optional equipment part numbers Thermal Printer The Thermal Printer provides archive quality 256 grayscale prints and five optional cost effective print sizes Thermal Printer Converter Plates The UV White Converter Plate allows imaging of non fluorescent stained media with an ultraviolet transilluminator The converter plate is specially coated to convert 302nm UV output to white light The Visi Blue Converter Plate not shown converts UV to 460 470nm and is designed for use with blue excitation samples such as SYBR Green SYPRO Orange and GFP stains UV White Converter Plate BioDoc It Imaging Systems 7 Setup Instructions Components BioDoc It Darkroom CCD Camera and Zoom Lens Camera Lens Mounting Bracket and Hardware Power Jumper and USB Cables Transilluminator USB Storage Device 1GB minimum WARNING DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS CONN2. select Printing Preferences 7 Choose the following settings High Density paper 1280X1280 Portrait mode 8 Click on the Option tab and click the Enlarge Image to Fit Paper box Then click Apply and OK 9 Restart the BioDoc it system If the thermal printer does not work correctly contact UVP Technical Support for assistance See the Technical Support section of this manual for contact information BioDoc It Imaging Systems 12 Service Procedures Return Procedure A Returned Goods Authorization RGA number must be obtained from UVP Customer Service before returning any product Replacement Parts and Accessories To order accessories or replacement parts for the BioDoc It Imaging System contact UVP s offices Part Description Part Number Filter Ethidium Bromide 50mm Square 38 0220 01 Filter SYBR Green 50mm Square 38 0219 01 Filter SYBR Gold 50mm Square 38 0221 01 White Light Converter Plate 21x26cm 38 0191 01 White Light Converter Plate 25x26cm 38 0191 04 Visi Blue Converter Plate 21x26cm 38 0200 01 Visi Blue Converter Plate 25x26cm 38 0200 04 Gel Cutter 85 0002 01 Gel Ruler 85 0003 01 Gel Scooper 85 0006 01 Gel Tray small 85 0007 01 Gel Sentry DNA Preparation Plate 97 0076 01 Fluorescent Standard Step Tablet 33 0014 02 Doc It LS Analysis Software 97 0185 02 Spectacles UV Blocking UVC 303 98 0002 01 Goggles UV Blocking UVC 503 98 0002 02 Faceshield UV Blocking UVC 80
3. 20561 Write to Ultra Violet Products Ltd Unit 1 Trinity Hall Farm Estate Nuffield Road Cambridge CB4 1TG UK Write to UVP LLC 2066 W 11 Street Upland CA 91786 USA Warranty UVP s products are guaranteed to be free of defects in materials workmanship and manufacture for one 1 year from date of purchase Consumable and disposable parts including but not limited to tubes and filters are guaranteed to be free from defects in manufacture and materials for ninety 90 days from date of purchase Transilluminators carry a two 2 year warranty from date of purchase If equipment failure or malfunction occurs during the warranty period UVP shall examine the inoperative equipment and have the option of repairing or replacing any part s which in the judgment of UVP were originally defective or became so under conditions of normal usage and service No warranty shall apply to this instrument or part thereof that has been subject to accident negligence alteration abuse or misuse by the end user Moreover UVP makes no warranties whatsoever with respect to parts not supplied by UVP or that have been installed used and or serviced other than in strict compliance with instructions appearing in this manual In no event shall UVP be responsible to the end user for any incidental or consequential damages whether foreseeable or not including but not limited to property damage inability to use equipment lost business lost profits
4. 3 98 0002 04 Troubleshooting No Power to the Darkroom or Transi luminator 1 Recheck the main power cord connection to the BioDoc lIt darkroom as well as the power cables between the darkroom and transilluminator Check the fuses located at the back of the unit next to the power port A flat head screwdriver will be required Turn the fuseholder cap counterclockwise and the fuse holder will pop out Inspect the thin wire within the glass fuse to see if there is a break in the wire If so replace the fuse s If fuses are blowing repeatedly contact UVP Technical Support for additional troubleshooting Transilluminator Will Not Turn On 1 Make sure to turn ON the two transilluminator power switches and that both switches are glowing green One switch is on the front of the BioDoc lt unit and is labeled UV Trans The other switch is located on the front of the transilluminator itself directly below the darkroom door If the switches do not glow green refer to No Power to the Darkroom or Transilluminator above Be sure the darkroom cabinet s door is completely closed There is a UV exposure safety cut off switch that turns the transilluminator off when the darkroom cabinet s door is opened Be sure the transilluminator s power jumper cord is securely connected at both the ends of the cable BioDoc It Imaging Systems 13 Touch Screen Not Accurate 1 If touching or clicking on the buttons becomes inaccurate the touc
5. BioDoc Ite Imaging System Installation and User Instructions UVP UVP LLC Ultra Violet Products Ltd 2066 W 11th Street Unit 1 Trinity Hall Farm Estate Upland CA 91786 Nuffield Road Cambridge CB4 1TG UK Phone 800 452 6788 909 946 3197 Phone 44 0 1223 420022 Fax 909 946 3597 Fax 44 0 1223 420561 Web Site www uvp com 81 0301 01 RevE BioDoc It Imaging Systems 2 System Introduction BioDoc It Imaging System The BioDoc lIt Imaging System enables simple documentation of fluorescent and non fluorescent gels membranes blots film plates and assays with the ability to save images to a USB storage device for later quantitative analysis or enhancement for publication Images are saved in 8 bit TIFF or JPEG formats allowing saved images to be read by most PC or MAC programs The BioDoc It Imaging System is a cost effective solution for capturing quality images ina compact standalone package as no external computer is required The BioDoc It comes in two configurations BioDoc It 220 Imaging System includes a high resolution 1 3 megapixel camera 8 48mm zoom lens and side access doors BioDoc It 210 Imaging System includes a 0 3 megapixel camera and an 8 48mm zoom lens BioDoc It Imaging Systems 3 System Components BioDoc It Components Refer to the packing slip and pictured components for parts included with the system FluorCam 210 or 220 Camera and Zoom Lens 8 LCD Touch Screen with Tilt C
6. ECTED TO THE POWER SUPPLY CAUTION Do not install the system in places with high moisture dust or high temperatures Do not use any oil or petroleum based cleaners on the cabinet Use only a mild soap or detergent solution for cleaning Ensure that the system is turned OFF and is unplugged from its power source during cleaning Keep the equipment away from motors or other large magnetic equipment apparatus Installing the Filter Systems include a 50mm sq Ethidium Bromide EtBr filter To install the filter in the BioDoc It darkroom 1 Carefully remove the filter from the protective plastic case holding the filter at the edges to prevent fingerprints 2 The filter tray is located under the camera bracket on the top right side of the darkroom Slide the tray open 3 Place the filter inside the filter tray Ensure that the text on the edge of the filter is positioned so it is right side up when facing the installer Additional filters are available Refer to Replacement Parts and Accessories for ordering information Camera Setup The camera and zoom lens are assembled at the UVP factory Note The zoom lens shipped may appear different than the one pictured at right 1 Remove the cap from the lens 2 If not already assembled attach the step up ring and diopter to the lens The step up ring and diopter will only fit one way BioDoc It Imaging Systems 8 3 Using the four brass thumb screws provided secure
7. apability Filter Tray located under the USB Drive a camera with EtBr Filter ba Cuan Main System Power Button Wide Access Door d UV Gel Viewer Window White Light and Darkroom Power Switches UV to White Light Converter Plate order separately E Side Access Doors on BioDoc lIt 220 models Transilluminator BioDoc lt Imaging Systems System Specifications Camera BioDoc It 220 Models The BioDoc lt 220 is equipped with a FluorCam 220 Camera resolution of 1 3MP BioDoc lIt 210 Models The BioDoc lt 210 is equipped with a FluorCam 210 Camera resolution of 0 3MP Both the FluorCam 220 and FluorCam 210 use a USB 2 0 PC connection All camera settings are factory pre set for optimum performance when viewing gels films or membranes under low light level conditions Contact UVP Technical Support before making any adjustments to the camera settings Lens The BioDoc It is equipped with an 8 48mm zoom lens fitted with a close up diopter and step up ring allowing the camera to be held in a fixed position The diopter is for focusing on objects at the focus length of the BioDoc It Imaging System s darkroom Built In Computer Internal Storage 60GB or more USB 2 0 Ports 1 front 4 in the back USB Storage Device Capacity 1GB minimum Software TS software Ethidium Bromide Filter EtBr The Ethidium Bromide EtBr filter 50mm sq UV blocking band pass interference fi
8. d brightness is achieved Readjust the Aperture adjustment ring if needed 4 Rotate the Focus adjustment ring on the lens Adjust this so that the image appears in clear focus on the LCD monitor Note Once the proper zoom range is set the lens will have to be refocused when zoomed in completely 5 Rotate the Zoom adjustment ring on the lens so that the image is as large as possible Readjust the Focus ring on the lens making the image clear Adjust the zoom so that the object of interest is within the picture on the LCD monitor Decrease Min Sec mSec Increase Integration Time Interval Selectors Integration Time Time Printer Setup The thermal printer is an optional accessory If this order did not include a thermal printer move on to the next section Otherwise to set up the printer 1 Plug the power cable from the printer into the power source 2 Press the Open button to open the lid of the printer and load the paper by allowing the loose end of the roll to come off the top of the roll After shutting the printer lid tear off the excess paper that is visible Plug in the USB cable from the printer into the BioDoc It USB port With the BioDoc lIt turned on press the Windows button on the lower right portion of the TS software screen 6 To set the printer settings click Start at the lower left of the computer Next click Printers and Faxes then right click on the appropriate printer icon and
9. ernal components will now have power and the TS software will automatically load To power down the system push the Main Power button Adjusting the Screen Angle 1 To adjust the angle of the screen loosen the black rubberized knobs on each side of the monitor Twist each knob counter clockwise note that the knobs will be turning opposite directions Tilt the screen to the desired angle Turn each knob clockwise to tighten Black Rubberized Knob SS Main Power Button BioDoc It Imaging Systems 10 Operating the TS Software Once the Main Power button is pressed the system will go through the boot up process Upon completion the LCD monitor will display the TS software screen similar to the one below To shut down the TS software touch the X on the bottom left corner of the screen To turn the system off press the Main Power button or power down from the Windows icon on the bottom right corner of the screen Refer to the TS Software User Manual for further instructions on using the software Using the Transilluminator Once the BioDoc It darkroom power is turned on power is supplied to UV Trans all components This includes the jumper cable that supplies power to the transilluminator To use the transilluminator turn ON the two transilluminator power switches One switch is on the front of the BioDoc It unit and is labeled UV Trans The other switch is located on the front of the tran
10. h screen may need to be recalibrated Please follow all instructions in the touch screen calibration instructions included in the TS software CD Error Messages Appear on the Screen 1 An error message that is related to the TS software interface or Microsoft Windows may appear on the screen If the message is related to Microsoft Windows such as a reminder to activate or update the copy of Windows please contact your system administrator for assistance 2 If an error message appears repeatedly and your system administrator does not recognize it as a Microsoft Windows error contact UVP Technical Support for further assistance Technical Support UVP offers technical support on all of its products If there are questions about the product s use operation or repair please contact UVP s offices at the locations below Or go to UVP s web site and click the Tech Support gt Biolmaging Systems If in North America South America If in Europe Africa the Middle East of East Asia or Australia Western Asia Call 800 452 6788 or 909 946 Call 44 0 1223 42002 and ask for 3197 and ask for Customer Service Customer Service during regular during regular business days between business days between 9 00 am and 7 00 am and 5 00 pm PST 5 30 pm E mail your message to ae techsupport uvp com E mail your message to uvp uvp co uk Fax Customer Service and send it to Fax Customer Service and send it to 909 946 3597 44 0 1223 4
11. lter blocks UV and IR radiation emitted from the transilluminator The filter is placed in the filter tray below the camera lens assembly The filter allows visualization of fluorophores from 580 630nm targeting the EtBr emission peak of 605nm The EtBr filter can be substituted for other specific fluorophore filters or removed when imaging non fluorescent media protein gels colony plates etc in order to produce brighter images BioDoc It Imaging Systems 5 Darkroom Darkroom features include UV gel viewer window Overhead white light m Wide access door with UV safety interlock switch Transilluminator A transilluminator is included with the BioDoc It system UVP offers a variety of transilluminators from benchtop models with 8 watt single wavelength and single intensity to models with multiple wavelengths and high low intensities UVP also offers the high end FirstLight a highly uniform 302nm UV transilluminator LCD Touch Screen The display is an 8 inch VGA color touch screen with tilt adjustment connected to the darkroom cabinet The touch screen allows the user to preview snap save and print images as well as select certain preference options without the need to use an external mouse or keyboard However should the user desire a mouse and or keyboard not included can be used with the BioDoc It A stylus pen is included for increased pointer control for selecting software options on the touch screen
12. or inconvenience arising out of or connected with the use of instruments produced by UVP Nor is UVP liable or responsible for any personal injuries occurring as a result of the use installation and or servicing of equipment This warranty does not supersede any statutory rights that may be available in certain countries BioDoc lt Doc It and FirstLight are registered trademarks of UVP LLC Visi Blue is a trademark of UVP LLC
13. silluminator itself directly below the darkroom door On Refer to the Transilluminator Manual for additional instructions on using the transilluminator Using the UV Gel Viewer Window The UV Gel Viewer Window has a metal cover to block out ambient lighting and to protect the UV safe glass The cover is held in the closed position by two small magnets built into the UV safe glass To open the window cover pull down on the metal tab The cover will pop open To close the cover pivot it up until it reengages with the two small magnets The glass is UV blocking while providing a clear UV safe view to the transilluminator surface for visibility of samples Metal tab BioDoc It Imaging Systems 11 Image Focusing Prior to capturing any images prepare the image focus capabilities of the system 1 Turn on the transilluminator and place the UVP Focus Target on the transilluminator surface NOTE The BioDoc lt darkroom has a UV safety switch that turns the transilluminator off when the door is open After closing the door be sure the overhead white light switch is turned OFF 2 Press the LIVE button on the TS software screen While watching the LCD screen rotate the Aperture adjustment ring on the lens so that the image is as bright as possible 3 Using the integration time settings at the bottom of the screen press the mSec time interval selector square Then use the button to increase integration time until the desire
14. the bracket to the base Camera bracket 4 Slide the camera and lens assembly into the camera bracket Slide the lens through the center hole in the gasket on the base and use the gasket to form a light tight seal around the lens _ a Camera USB cable Camera and lens assembly Brass thumb screws secure the bracket to the base 5 Insert and tighten the black thumb screw and washer in the central hole in the camera to secure the camera to the bracket 6 Plug the camera cable into the top of the camera and the other end into a USB port on back of the darkroom Connection for the main darkroom power 7 Connect the jumper cord from the darkroom to the transilluminator Connection for jumper cord to the transilluminator 8 Connect the main power cord from the back of the darkroom to a surge protected power outlet Stylus and Holder The BioDoc It screen is touch sensitive Use the stylus included with the system to operate the software controls For easy access to the stylus when operating the system remove the tape from the back of the stylus holder and adhere it to the BioDoc It Slide the stylus into the holder BioDoc lt Imaging Systems Using the System Powering Up the System 1 Ensure that the main power cord is connected from the back of the darkroom to a surge protected power outlet Power up the system by pressing once on the Main Power button located on the right side of the unit All int
Download Pdf Manuals
Related Search