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TRobot user manual

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1. 6 10 1 Examples Query of block temperature b 1 l 6 Bytes in Mnemonics BLCK 1 TEMP 12 Bytes 6 11 Error codes Error codes consist of three decimal numbers The first sign determines the error group Variable numerical values of several formats in this description are represented by format signs beginning with Examples 4x hexadecimal 4 digit 2d integer 2 digit 5f1 decimal value signed 5 digit altogether consisting of 3 integer digits decimal point and 1 post decimal digit All error texts end with l See also chapter 6 2 3 2 Special error code 1 checksum error 6 11 1 Error Group 0xx System Errors 001 remote control off remote control not activated 002 Error 4x 4x 4x 4x 4x 4x 4x software error system halted 003 wait too cold cooler too cold 004 cooler too hot cooler too hot 005 no printer no printer available 006 no heated lid heated lid not connected 56 Instruction Manual TRobot 10 2009 007 008 009 010 6 11 2 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 printer active 1 2d only 1 2d only handshake not possible Error Group 1xx Edit Errors max 1d max 2d edit not possible pgm full 2d steps pgm is empty pgm is active pgm is edited RAM full only 3d programs possible max 2d min 1 max
2. Note For analysis of temperature data the file Temperature_profile_ThermocyclerManager xls see chapter 5 can be used 5 3 3 Connect Thermocycler to the Thermocycler Manager Software After the network setup is completed Thermocyclers can be addressed by the Thermocycler Manager Software Press the button 100e in the Thermocycler Manager Software main screen see chapter 5 3 After a connection is established the type of Thermocycler that is Instruction Manual TRobot 10 2009 25 biometra a part of Analytik Jena controlled and the status of various parameters are displayed in blue by the Thermocycler Manager Software Moreover different buttons that previously were grayed out and therefore inactive see figure chapter 5 3 now have become active and can be used B Biometra Thermocycler Manager 4 11 Cycler Thobot Com 1 Settings ucler TRobo o a Start Sege ER Block Fause in Director g 1 GG inactive e Continue Name Test 3 Gs Block info Shop View pgm Tbemmocucler TRobat Block 1 Is off Block temperature SOUL Lid temperature 61 6 C Active directory Active program Time Step Remaining time Program es Memory management 5 3 4 Block Info Press button Fleck inte to receive information on the block type of the Thermocycler that is currently addressed by the software Block type C Standard 96 C Standard 48 C Standard 384 C Combi C Combi 2048 C Gradient 96 Standard 20
3. Instruction Manual TRobot 10 2009 2 biometra a part of Analytik Jena loj x New pom Load pom Directory number 8 Program number Jo Lid temperature Jo sr Preheating Iw on Frogram name Step Block Loop Temp Time number temperature Time Goto number inc inc fp epeka ee E Poep ep e Slope Delete step Insert step Save as tert There are a number of input fields for various parameters available First define the directory number program number lid temperature lid preheating and assign a program name Then enter parameters for the first program step Goto This parameter specifies the starting point of a loop Loop number This specifies how many times a cycle is run total cycle number number backloops 1 Temp inc Temperature increment This option allows increasing decreasing temperature within a cycle Time inc Temperature increment This option allows increasing decreasing dwell time within a cycle Slope This parameter specifies the maximum ramping rate value 5 means maximum heating and cooling Confirm your entries by pressing the button SE The parameters of the first step will be displayed in a spreadsheet and the next program step can be entered Step Block Loop Temp Time number inc inc er MM cP P ap Pf po ef sfr Time Goto Slope number t enp erature 5 4 2 Edit program To open a program stored on TRobot Thermocycler enter program number and directory num
4. MTAS_O_3 ENSCH RAMPE_O_6 Im DyZ 35 _0_7 RAMPEHD_O_9 Abbrechen After copying you receive a message how many programs have been copied to the selected folder Thermocycler Manager Wi 10 programs From cycler TPersonal on com 1 copied 34 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 5 5 7 Transfer programs from PC to Thermocycler Press button Dawnload programs tram PL in the window for memory management see chapter 5 5 In the next window specify which programs to be transferred to the Thermocycler Select File s for Send _ x Suchen mr E Temperature_Programs de t m be HKRater_0_10 B TREPPE_O 4 al Holger i MTAS_O_3 NOP_1_1 RAMPE_O_6 F RAMPEHD_O_3 D ateiname Dateityp Temperature Programs Files mb ll Abbrechen Note If you would like to select multiple programs at one time hold down the Ctrl key during the selection process E Press Upen to confirm your selection If a memory location is already in use you will receive a message If the existing program shall be overwritten confirm with lt yes gt otherwise press lt no gt Thermocycler Manager Eu Replace existing program D steps Finally you receive a message how many programs during the process have been copied Thermocycler Manager 1 programs From PC to cycler copied 5 5 8 Create Service Info File This function creates an information file
5. 25 5 3 4 BIOER III One seen E 26 9 3 9 ie BE 27 5 4 Programming SCHEIN ana ee ren 27 5 4 1 CIE BIO AN ea ee eer ee esse 27 5 4 2 EDOT AT et ee 28 5 4 3 Beie elge OAM ee ee ee 29 5 4 4 Save elle MO EE 29 5 4 5 Send program to Thermocvcler nenn nano nenne nnnennnenennenen 30 5 4 6 STALL OVO GANA eege 30 5 4 7 PAUSE ele ell EE 30 Instruction Manual TRobot 10 2009 3 biometra a part of Analytik Jena 5 4 8 SOP Ol OGL AN E 31 5 4 9 Show remaining time cece ceccc cece eeeece cece eeceeeeseeeceueeseeeseeeseeesseeeseeesaeeeseeeseneeaes 31 5 5 Memory manageme Mi EE 31 5 9 1 FIN DIOG EI tee ee S 31 9 9 2 VIEW Progra een ee reed 32 5 5 3 CODY Droga WE 32 5 5 4 Delete program 33 9 9 8 RT ge ele een esse nee 33 5 0 6 Save all programista as Cane eee eee ee ee ee 34 5 9 7 Transfer programs from PC to Thermocvcler 35 5 9 8 Create Service MINTO FINS unse 35 5 5 9 ECE COM In Le EE 36 5 5 10 Exit memory management window 36 5 6 Exit Thermocycler Manager Gotbware 36 5 7 Analysis of temperature data 36 Serial Communication Protocol uurs2uu20202an000nannunnnnnunnnnnnunnnnnunnnnnnnnnnnnunnnnnnnnnnnnn 39 6 1 ENEE teg le EE 39 6 2 Asynchronous Communication 39 6 2 1 GENE Al gebeten 39 6 2 2 COMANA ier d EE 39 6 2 3 Command OY siropa ee en re ee eine 40 6 2 4 Spontaneous Error Messages nenne nnnenenne nenn nnnnennnenn 42 6 2 5 Menu Structure cccccc cece sr rianne EEEN EEPOSE EENAA 43 6 3 Synchronous tran
6. ventilation slots Figure 4 TRobot front view 18 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 4 2 The TRobot rear view front locking unit 96 well silver block er E wheel for plate lifter e adjustment of ee M a lid pressure plate lifte nn ventilation slots port for E power cable contact for CH gt emergency release GEN __ of motorized lid N port for use only 9V block battery controller cable 37 pin Figure 5 TRobot rear view English Deutsch SSS Only use a 9V block battery to open Verwenden Sie ausschlie lich eine the TRobot lid manually 9V Blockbatterie um den TRobot Deckel manuell zu offnen 4 3 Initializing motorized lid pressure The TRobot lid pressure can be set in 15 increments The maximum pressure on the whole block is 10kg Before using the motorized pressure lid it has to be adjusted to the type of plasticware Because the plate dimensions of different suppliers significantly vary the lid has to be adjusted to the ZERO position no pressure turn knob to 0 Always unplug the power cable before you adjust the lid pressure of the TRobot Vor dem Justieren des TRobot Heizdeckels immer den Netzstecker ziehen Instruction Manual TRobot 10 2009 19 biometra a part of Analytik Jena Never put your hands inside the instrument during the motorized lid is in action The closing lid exerts significant pr
7. 2d min 4f1 max 5f1 min 5f1C max 5f1C max 2h 2m 2s max 2d too many nested cycles back cycles only max 2d cycle into cycle cycle out of cycle gradient out of temperature range YotiC 5f1C max 2d C max 3d s value cycle value 6 11 3 Error Group 2xx Block Errors biometra a part of Analytik Jena printer is already active paper feed 1 2 or 3 display contrast communication cable not suitable max directory number max program number program is active max number of steps reached program is empty program cannot be changed Editing of this program isn t finished memory full max number of programs exceeded step number larger than progr length 1 step number too small step number larger than progr length heated lid temperature out of range block temperature out of range gt max time exceeded max number of back cycles exceeded too many nested cycles destination step is larger than start step cycle into cycle cycle out of cycle temp out of range because of gradient gradient out of range temperature increment too large time increment too large step incomplete temperature time back cycle destination or number of back cycles missing In the long format after the error text or in the short format after the error code follows the block number blocknr b 1d controller number cir
8. Before switching on the instrument make sure that the instrument is fully assembled see chapter 3 4 3 4 Installation Conditions e Place the TRobot Thermocycler on a stable surface in a dry environment e Let equilibrate the TRobot Thermocycler to room temperature before starting operation 1 to 6h e Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the controller from the mains e Make sure that the ventilation slots at the front and the rear of the TRobot are not obstructed Make sure that there is no object in front of the Thermocycler that may block the ventilation slots at the front e g a piece of paper etc e There must be sufficient distance between the ventilation slots on the rear side of the Thermocycler and a wall or another instrument min 10 cm Ensure that the ventilation slots at the front and back of the instrument are unobstructed Die Luftungsschlitze an der Vorder und Ruckseite mussen freigehalten werden Insufficient ventilation can cause overheating of the instrument Eine unzureichende Bel ftung kann zu einer berhitzung des Ger tes f hren e Make sure that the main supply voltage is in accordance with the label above the power connection see section 3 3 e Connect the TRobot Thermocycler and controller to a grounded socket e For installation of the TRobot connect controller an
9. C Gradient 48 C Standard 48a Additionally in the main screen an information is displayed which blocks are currently active This feature is especially interesting for multi block systems like the T3000 Thermocycler that may also be controlled by the Thermocycler Manager Software Since the TRobot is not a multi block system while in use always block 1 is set active Block 1 Ge active E Sab 26 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 5 3 5 Lid By activating the button Lid 1 anew window opens providing the functions for controlling the automatic lid Lid it heating and closed Press ren o open the TRobot lid Press 08 to close the TRobot lid Press Eu to close the window Moreover a status line is displayed below the buttons giving information on the lid status Note See chapter 7 4 how to open and close the lid off line EEE English Never put your hands inside the Beim Schlie en des Deckels niemals instrument during the motorized lid is die H nde in den Thermocycler in action The closing lid exerts halten Durch die Kraft des Deckels significant pressure and can cause besteht bei unsachgem lfer bruises when operated improperly Handhabung die Gefahr von Quetschungen 5 4 Programming screen 5 4 1 Create program To create anew a program press button New in the Thermocycler Manager Software main screen see chapter 5 3 3 The programming screen opens
10. Please keep the original packing material for return shipment in case of service issues A I Attention A Please fill out and send back the warranty registration card This is important for you to claim full warranty 3 3 Operation Voltage The TRobot Thermocycler can operate at 100 115 or 230 Volt Important Prior to connecting the controller to the mains make sure that the setting of the Voltage selector at the backside of the controller is in accordance with your mains Voltage and that it is equipped with the correct fuses Backside Controller Backside Controller Voltage Indicator X Me Fuse holder Adjustement slot Mains supply 2 Figure 1 Voltage adjustment slot Figure 2 Fuse holder 1 To change the operation Voltage settings switch off the instrument and disconnect the mains plug Instruction Manual TRobot 10 2009 15 biometra a part of Analytik Jena 2 Use a coin or another round shape item to turn the adjustment slot of the Voltage selector at the backside of the controller see figure 1 to the new Voltage setting 3 Pull out the fuse holder at the backside of the controller see figure 2 Load the fuse holder with two fuses corresponding to the designated operation Voltage At an operation Voltage of 230V use two slow blow fuses of 2 Ampere 2x 2ATT at an operation Voltage of 100 or 115 Volt use two slow blow fuses of 4 Ampere 2x 4ATT 4 Reinsert the fuse holder 5
11. a or only the if there is no data for transfer to the master 6 3 1 Data format of synchronously transferred data separator After the identifier follows a blank The data is separated by a comma ASCII presentation internal format value separator buffer Format Bytes Bytes Bytes Bytes identifier 1 1 1 2 block NR 1 to 2 1 to 3 block status 2 to 4 1 to 5 actual time 4 to 8 1 to 9 hold time 4 to 8 1 to 9 step NR 1 to 2 1 to 3 loop counter 1 to 2 1 to 3 0 2 to 4 1 to 5 44 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena heated lid temperature 2 to 4 1 to 5 heat sink temperature 2 to 4 1 to5 format identifier 1 1 1 2 temperature data depends on the format identifier actual time counter which is incremented each 64ms hold time Increment every second Shows the hold time from the beginning of a temperature plateau or during a break the time of this break format identifier of temperature data A middle temperature 2 to 4 Bytes counter of the innermost loop The termination of synchronous data is marked by lt CR gt and if tuned in in front of each terminating sign the CRC16 check value The format of the data is flexible Leading zeros are not transferred Example _1 23 84C7F3 54 B 3 0 230A 10A4 A 1B58 Explanation 1 0023 8701939 64ms 84s 11 3 0 89 70 C 42 60 C A 70 0 C 6 3 2 Sending only
12. a long command the interpretation switches to long commands As a result error messages will be sent also in the long format see chapter 6 11 The use of any short command switches the communication back into the short format 6 2 3 Command Reply 6 2 3 1 Standard Reply A command reply starts with the short command code with a capital letter without distinguishing between the short and long format The format of the parameters depends on the form of the last command which was sent With each transferred data block only one reply is sent reply code _parameterT parameterz CRC 16 lt CR gt reply code command code with a capital letter blank 40 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena parameter parameter according to the list of instructions CRC16 check code optional lt CR gt ASCII Carriage Return OD If a reply is sent with parameters after the reply code there will follow a blank and then one or more parameters see chapter 2 2 Description of Commands Example command a lt CR gt HEAD menu EDIT reads the program head data reply A_ 63 1 TESTT lt CR gt heated lid 99 C heated lid preheating on program name TEST1 6 2 3 2 Error Message ve is Error identifier error messages always have the sign as error identifier detection and a following three figure error code in HEX short commands are replied from the cycler by error messages in
13. chapter 5 4 5 how to send a file to the Thermocycler 5 4 5 Send program to Thermocycler To send a saved program to the Thermocycler press button Send in the Thermocycler Manager Software main screen see chapter 5 3 Choose the program to be transferred and press Iren D ateiname P Datetup Temperature Programs Files tpb Abbrechen Note The program store directory and program number is pre defined in its parameter list see chapter 5 4 and can not be selected before transmission If you would like to save the program to a certain store number first specify the desired location in the parameter list and save the program prior to transmission EZ 5 4 6 Start program To start a program first enter the program and directory number in the Thermocycler Manager Software main screen Program m in Director g Mame ASA View pgm Note The name of the program will be displayed automatically To check the program prior to start press button en pam Press button tart in the Thermocycler Manager Software main screen see chapter 5 3 to start the program 5 4 7 Pause program To pause a program press button F in the Thermocycler Manager Software main screen see chapter 5 3 Press Long to resume the started program 30 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 5 4 8 Stop program Press button eP to stop the program in the Thermocycler Manager Soft
14. limited non transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under one or more of US Patents Nos 5 038 852 5 656 493 5 333 675 5 475 610 and 6 703 236 or corresponding claims in their non US counterparts owned by Applera Corporation No right is conveyed expressly by implication or by estoppel under any patent claim reagents kits or methods such as 5 nuclease methods or under any other apparatus or system claim including but not limited to US Patent No 6 814 934 and its non US counterparts which describe and claim thermal cyclers capable of real time detection Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA Biometra biomedizinische Analytik GmbH Rudolf Wissell Str 30 mR 37079 Goettingen Germany icensed and quiharized 1 4 2 Copyright All rights reserved It is not allowed to copy and publish the manual or parts of it in any form as copies micro film or other methods without a written authorisation from Biometra Biometra is pointing out that applied company and brand names are usually protected trade marks 1 4 3 Liability Biometra is not liable for damages and injuries caused by use not considering these operating instructions in parts or completely 1 4 4 Meaning of the Instructions Bio
15. step With parameter 1 and 2 sets all step data 0 htime loop loops 30 hex defaults Joop 0 loops 0 no back loop loops 0 tempi 0 cycle tempinc 0 timeinc 0 slope maximuminc timeinc slope tempinc tim slope ei nc siopell l d STPS number Sends number of steps of edited program steps of steps e ISTP stepnr insert stepnr must not be more than the previous step bltemp htime step number 1 0 30 hex defaults b temp loop loops 50 00 C htime 30 s loop 0 loops 0 no 0 back cycle tempinc 0 timeinc 0 slope tempinc tim maximum slope ei NC slonel f DSTP stepnr delete stepnr must not be more than the previous step step number g EEND edit end gt UM LIBR Ends editing and returns to LIBR h RUNT runtime Sends runtime of edited program runtime 6 9 9 Menu Protocol PROT co mnemo parameter description reply parameter a CHCK checktype check Data transfer check checktype type 0 without CRC16 check value 1 with CRC16 check value without parameter sends check type b ECHO echoflag echo 0 without echo echoflag type 1 quasi echo without parameter sends echo type c WATM waittime waiting Waiting time for the repetition function of CRC waittime time without syncdata It is active only for once command and for more commands with inactive Values between 0 and 254 64ms 52 Instruction Manua
16. the foils Biometra will not be responsible for transport damage by improper packing Always use two separate boxes for the shipment of the controller and the TRobot instrument to prevent damages Gerat zwischen den Folien schwebend einspannen GZ SE AS Mount instrument floating in between the foils Instruction Manual TRobot 10 2009 65 biometra a part of Analytik Jena 11 Equipment Decontamination Certificate To enable us to comply with German law i e 871 StriSchV 817 GefStoffV and 19 ChemG and to avoid exposure to hazardous materials during handling or repair please complete this form prior to the equipment leaving your laboratory COMPANY INSTITUTE ADDRESS PHONE NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluation Start Date Finish Date Hazardous materials used with this equipment Method of cleaning decontamination The equipment has been cleaned and decontaminated NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER 66 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena General Information for Decontamination Please contact your responsible health amp safety officer for details Use of ra
17. the short format long commands are replied by error messages in the long format see chapter 6 11 Command codes with errors short form error code_command block CRC16 lt CR gt long form error code error text_command block CRC16 lt CR gt error code error identifier see error list _ blank command block invalid command block error text plain text error message English CRC16 check code optional lt CR gt ASCII Carriage Return OD A command with an error results in a reply with an error identifier a blank followed by the error text with a terminating blank using the long format and the invalid command block Optionally the checksum may be transferred in front of the terminating sign Example I501_k 78 lt CR gt short form 1501_ invalid command _ k 78 lt CR gt long form Parameter short form reply code_ error code_parametef CRC16 lt CR gt long form reply code_ error code_ error text_parameter CRC16 lt CR gt reply code command code in capital letter Instruction Manual TRobot 10 2009 41 biometra a part of Analytik Jena _ blank error code error identifier see error list parameter invalid parameter error text plain text error message English CRC16 check code optional lt CR gt ASCII Carriage Return OD A long command with an incorrect parameter receives a reply in the long format otherwise in the short form The reply code is identic
18. 1 not possible syO read denied reading from transmitting buffer 1 not possible Instruction Manual TRobot 10 2009 623 631 632 633 641 overflow syO sy1 write denied sy1 read denied overflow sy1 sync data fault Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena overflow of the transmitting buffer O writing into transmitting buffer 2 not possible reading from transmitting buffer 2 not possible overflow of the transmitting buffer O writing into transmitting buffer 1 and 2 not possible 59 biometra a part of Analytik Jena 7 Trouble shooting 7 1 Slow heating and cooling See chapter 0 7 2 Autorestart Running programs will be continued from the same step where the power failure has happened as soon as the power is supplied again In the Thermocycler Manager Software main screen see chapter 5 3 a message is displayed showing that the TRobot has been restarted Block 1 is restarted 7 3 Autorestart due to unrecognised power failure High voltage fluctuation can lead to an automatic restart of the TRobot Thermocycler In this case the cycler restarts at the step where there power failure has occurred A message is displayed showing that the TRobot has been restarted see chapter 7 2 To avoid voltage fluctuation do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a centrifuge 7 4 Opening and closing heated lid off line The mot
19. 9 1 4 1 PCR License Legal Dscdamer 9 1 4 2 GOP MORT ee ee reinen 9 1 4 3 Brei Uh ote ese tc ese ance does anise eee BUN SEN A NEN E E NER E N E NEE ERANAERE 9 1 4 4 Meaning of the Instructions 0000 0nnn0 nenn ennne nenne nenn namen nnne nennen 9 2 Safety and Warning Notices uu2 002a00000n nnn00nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnunnnnnnnnnnunnnnnnn 10 2 1 DENAN OOTY PMO ONS E 10 EVAN ANON EE 15 3 1 KEE EE 15 3 2 Uppackand NS ER ana nor acon ese tethers a bate ee EHE Nee nen 15 3 3 Operation Voltage nano nenn nano nenne nano nnnn nenne nenn nano nenne nnnennnennnne nennen 15 3 4 Installation Conditions aannaaneannannnnnnennannnnnnnnnnnnonrnnrnnrrnrrrnrrnnrnnrrnnrenrnrirerrenrenrrerrnnene 16 4 Operating ClEIMC CC 18 4 1 The ERODOE ION VIEW ee ee ep 18 4 2 Toe Kee TEA VOW ee ee ee nee 19 4 3 Initializing motorized lid pressure nenn nenn anno anne anne anne ann nnn nenn nennen 19 4 4 Adjust TRobot lid to zero position 20 4 5 Setting lid Beil 21 5 Thermocycler Manager Softwar e cccccssssecsssssseescsssseesccesseeeccenseeeeenseeesenseessosseesees 23 5 1 A OCU de ee ee ee E 23 5 2 Installing the Thermocycler Manager Software nnannnnnnennannnnnnnnnnnnnnnnnnnnnnnnnnennenne 23 5 3 The Thermocycler Manager Software main screen 23 5 3 1 Network ue LN 23 5 3 2 Record temperature profle nenn ennne nenn sans 24 9 3 3 Connect Thermocycler to the Thermocycler Manager Software
20. Details The TRobot Thermocycler must not be used with explosive flammable or volatile liquids Der TRobot Thermocycler darf nicht mit brennbaren explosiven oder fl chtigen Substanzen betrieben werden Instruction Manual TRobot 10 2009 gt gt PPP gt gt Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena Do not place fingers between lid and housing when opening or closing the lid Beim Offnen und Schliessen des Deckels keine Finger zwischen Block und Deckel bringen Gefahr von Quetschungen It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes If you still decide to use oil do not use silicon oil Mineral oil may be used Die Verwendung von l zwischen Proben und Block f r einen besseren W rme bergang ist nicht erforderlich Sofern Sie dennoch Ol einsetzen wollen auf keinen Fall Silikon l sondern Mineral l verwenden Ensure that both the rear and bottom ventilation slits not clogged by dust or other material Danger of overheating Stellen Sie sicher dass die L ftungsschlitze an der Unter und R ckseite frei zug nglich und nicht durch Staub oder andere Materialien verstopft sind Gefahr der berhitzung Let equilibrate the TRobot Thermocycler to room temperature before starting operation Insofern der TRobot transportiert wurde das Ger t bitte vor dem ersten Anschalten auf Ra
21. EMP _ temp Sends current block temperature ee ra lid Sends current heated lid temperature ee cycle Sends current counters of all open loops of the cyclcount cycl counter current step starting with the innermost Without count cyclcoun reply parameter no loop f RUNS running Sends directory program step number and all set dirnr orognr pr step values of the current step If direct mode active jogname lidtem sends invalid value FFhex of directory and p preheating program numbers Nothing if the block is off stepnr bltemp h time loop loop s 0 tempinc tim ein c slope r REMT remaining Sends remaining time of active program if the remtime time direct mode is active then 0 50 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena u AUTR aurestepnr cy clcount cyclco ntl cyclcount autorestart Sends step number and all cycle counter of data program run at the moment of the last restart Counters of all open loops starting with the innermost If the direct mode is active then sends step number 0 Without reply parameter Block was off during restart or data were cleared when block was startet once more 6 9 5 Menu Block Setup BLCK BSET co jmnemon paramete description reply a BTYP e mern Sets block type Without parameter sends block type blocktype e ype b INTV interval interval interval seconds Sets measu
22. OT f CHCK a ECHO b WATM IC ITOR d HTMP LOOP p HWHS e DNAM e RUNS q REMT r AUTR u EDIT EDIT a HEAD a STEP b NSTP c STPS d ISTP e DSTP f EEND g RUNT h Block Setup BSET b BTYP a INTV b STMP c BDAT d 6 9 2 Description of Commands SM Change to submenu UM Return into the upper menu 6 9 3 Main Menu Parameter is optional comimnemonicparameterdescription reply a SSTT 0 status Sends system status and switches off the present systatus alarm off jacoustic signal With O all error flags of sysstatus apart the block error flag are cleared The block error flag is automatically cleared by starting the next program or restarting the cycler software b BLCK blocknr block gt SM BLCK blocknr number Current block number is saved up to the next change c LIBR library gt SM LIBR Instruction Manual TRobot 10 2009 49 biometra a part of Analytik Jena d INFO information gt SM INFO f PROT protocol gt SM PROT j ARM alarmfll set clear 0 without acoustic signal 1 with acoustic signal aarm alarm flag 6 9 4 Menu Block BLCK com mnemonic Mmnemonicparameter description reply parameter BSTT block status Sends block status Controller error flag and bistatus autorestart flag are automatically cleared by starting the next program or restarting the cycler
23. One Time Synchronous transferable data can be sent from the cycler after the demand by the master only one time see SDAT command in menu BLCK In this case the reply begins with E in place of HP 6 4 Setting of the Transfer Protocol interface configuration 9600 Baud 8 Bit no parity 1 Stop bit data format US ASCII sign handshake RTS CTS switchable at the cycler echo mode Quasi Echo switchable at the cycler buffer size 124 signs for each buffer sending and receiving buffer check process CRC16 according to ISO 3309 switchable Instruction Manual TRobot 10 2009 45 biometra a part of Analytik Jena 6 5 Communication Start and Stop The cycler only sends data if the master is ready and signals this by sending a sign to the cycler 6 5 1 Power up of the cycler Connection start During the power up of the TRobot the cycler stores one or more messages for the master After the cycler receives the first sign from the master connection start the saved messages of the cycler are transferred to the master before the cycler evaluates and replies the message to the master Check process CRC16 and long commands are deactivated after the initialisation of the cycler which results in a switch on message without a check value in the short format The first message which is stored by the cycler is always the message of the protocol version 1000_ protocol version lt CR gt _ blank protocol version version number of the c
24. Turn the metal ring to alter the height of the internal lid A special tool is included Turn the ring three times clockwise to raise the lid Turn the metal ring counter clockwise to lower the internal lid until you can feel that the lid has just come in contact with the plate on the thermoblock This is the defined zero position direct contact between plate and lid but no pressure fix release Hold the metal ring tight eventually use a pliers to fix lid height and screw hexagonal screw clockwise to fix the position of the inner lid u a emm STT Figure 6 Setting lid pressure Instruction Manual TRobot 10 2009 21 biometra If evaporation occurs despite elevated pressure please choose different brand of plastic ware Since microplate dimensions vary between manufactures not every plate may be compatible with the instrument Perfect fit between block and plate is essential for trouble free operation Biometra recommends 96 well plates 050 232 and 384 well plates 050 231 Never dose liquid to the PCR microplate inside the TRobot Dose liquids outside of the instrument and always use already sealed PCR microplates Keine Fl ssigkeiten innerhalb des TRobots in die PCR Mikrotiterplatte dosieren Dosieren Sie die Fl ssigkeiten au erhalb des Ger tes und verwenden Sie immer bereits versiegelte Mikrotiterplatten 22 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jen
25. a 5 Thermocycler Manager Software 5 1 Introduction The Thermocycler Manager V4 11 is a PC Software designed to address the following Biometra Thermocycler models T1 plus TRobot T3000 und TPersonal Up to 16 Thermocyclers can be connected and controlled in a network The software allows to create modify and store programs that can be exchanged between different Thermocyclers Since the TRobot is designed for integration in workstations the cycler will normally be controlled by the software of the robotic station For this purpose the cycler has to be integrated in the workstation software Integration will be done by the manufacturer of the workstation For a detailed description of all commands see section 6 9 5 2 Installing the Thermocycler Manager Software To install the Thermocycler Manager Software execute the file Setup hermocyclerManager exe and follow the instructions of the installation Wizard All software drivers will be automatically installed 5 3 The Thermocycler Manager Software main screen After the Software is started the Thermocycler Manager main screen is displayed that allows access to all functions B Biometra Thermocycler Manager 4 11 Com Settings Lid Stan ai Black Pause in Directory m I 7 2 7 Sab we Eontinue Name Block mto Stop je DOM Thermospeler P Block i Block temperature 7 Lid temperature 7 Active directory Actve program Time Step Remaining time Program r Me
26. al in both forms The error message consists of the reply code command code with a capital letter a blank an error identifier a blank in the long form the error text with a following blank and the erroneous parameter Example E 112_ FE lt CR gt short form E 112 max 5 FE lt CR gt long form Other errors short form reply code_ error code CRC16 lt CR gt long form reply code_ error code_ error textfCRC16 lt CR gt Reply code command code in capital letter _ blank error code error identifier see error list error text plain text error message English CRC16 check code optional lt CR gt ASCII Carriage Return OD After a long command the error message returns in the long form otherwise in the short form The reply code is identical in both forms The error message consists the reply code command code with a capital letter blank error identifier as well as in the long form the error text separated by a blank Example E 108 lt CR gt short form E 108 RAM full lt CR gt long form 6 2 4 Spontaneous Error Messages Spontaneous error messages are not connected with a precursory command of the master They are not a reply to a command short form error code btip CRC16 lt CR gt 42 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena long form error code_error text btip CRC16 lt CR gt error code error identifier see error list u blank err
27. al the cycler gives only a status signal after the request of the master Exceptions a Spontaneous message of the cycler If the cycler detects an error by the use of its security system e g overheating the cycler sends a spontaneous error message to the master if the connection to the master is still online see chapter 6 2 4 b Synchronous data see chapter 6 3 Next data block Before a new data block can be transferred to the cycler the reply relating to the last data must be received by the master or else the cycler sends an error message 6 2 2 Command Structure General output format command block 1 command block2 command block3 CRC 16 lt CR gt command block command code and parameter CRC16 CRC16 Check value optional lt CR gt ASCII Carriage Return OD Instruction Manual TRobot 10 2009 39 biometra a part of Analytik Jena The elements in angular brackets are optional A transmission block may be constructed of multiple command blocks which are separated by Each command block terminates with lt CR gt If the check process is activated a check value will be sent in front of each termination signal see chapter 6 6 1 Each command block has the following format command code _parameterT parameter2 command code command code blank Parameter parameter according to the list of instructions If parameters were sent in combination with a command a blank
28. am source dirnr Progi Instruction Manual TRobot 10 2009 51 biometra a part of Analytik Jena d NPRG dirnf next Sends directory and program number of next existing dirnr prognr existing program in order of the numbers If no number was saved program before yet e g by editing the search starts with directory 0 program 0 With dirnr the start directory can be determined The search ends with the last program in the last directory e g directory 9 prg 99 Without reply parameter no program is found e DNAM dn directory Without directory name sends directory name With dirname dirname name directory name sets directory name 6 9 8 Menu Edit LIBR EDIT co mnemo parameter description reply parameter a HEAD flidtemp program Without parameters sends program header data idtemp preheating preheating head With parameters sets all program header default progname progname lidtemp 0 without lid heating preheating 1 b STEP stepnr edit Without any optional parameter sends step data bltemp htime loop bltemp read With parameter 1 3 sets all step data 0 30 loops 0 tempinc htime step hex defaults oop 0 loops 0 no back timeinc slopel loop loops cycle tempinc 0 timeinc 0 slope maximum 0 slope c NSTP bitemp next Without any optional parameter sends step data stepnr bltemp htime
29. ame and location of the files can be defined Speichern unter Speichern E cycl ans 13h35m5e s 15 7 2009 E cycl ans 17h30m45s 13 2 2008 Dateiname Joycl_ans_O8h47m54s_1 B24 2009 Dateityp Text Files txt M Abbrechen i The data format of the stored files can subsequently be changed Choose under Temperature data conversion the appropriate format by activating the corresponding radio button see figure 8 chapter 5 3 1 The temperature data can either be displayed separated by a point or a comma Then click the button Fett and in the new window that opens select the file to convert 24 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena Suchen mr E Data d t ES cycl_ans_13h35m52s_ 135 7 2009 E cycl_ ops 17h30md5s_ 15 3 2008 Dateiname SEE Dateityp Text Files D txt Abbrechen A Click on Hpen to confirm your selection A new window opens where the name and location of the converted file can be defined Speichern Convert_Data den t S EEE be E cycl ans 15h35m5e s 135 3 2009 tempdat 1 Dateiname RER Dateityp Ten Files tet sl Abbrechen we Click on lt save gt to complete the process After the file is converted a message window with the location and name of the file is displayed Thermocycler Manager Ki 1 Converted data are saved in the file Programme Biometral Thermacycler Manager Convert Dakalcycl ans 13h355m52s 15 2 2009 bempdat_1 txt
30. and und Liechtenstein Cet symbol conteneur a d chets barr d une croix signifie que le produit en fin de vie doit tre retourn a un des syst mes de collecte mis a la disposition des utilisateurs finaux en consequence des regulations par la loi de votre pays Pour des information additionel nous Vous demandons de contacter votre distributeur Cet symbole s applique uniquement aux pays de EEE EEE Espace conomique europ en qui regroupe les Etats membres de UE et la Norv ge Islande et le Liechtenstein 68 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 13 Declaration of Conformity EU Konformit tserkl rung EC Declaration of Conformity G ttingen August 2004 im Sinne der EG Richtlinie ber elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 73 23 EWG following the EC directive about electrical equipment for use within certain limits of voltage 73 23 EEC und and im Sinne der EG Richtlinie f r die elektromagnetische Vertr glichkeit 89 336 EWG following the EC directive about the electromagnetic compatibility 89 336 EEC Hiermit erkl ren wir dass folgende Thermocycler Herewith we declare that the following Thermocyclers Typen types TRobot Thermocycler 96 TRobot Thermocycler 384 Best Nr Order No 050 991 050 992 den grundlegenden Anforderungen der corresponds to the basic requirements of EG Niederspannungsrichtlinie 73 23 EWG un
31. apter 6 9 2 Example of a command sequence in the short form editing of a programme cja_3 2 a_ 63 TEST13 b 1 251C 1E c_1388 1E g lt CR gt explanation C LIBR main menu goes to menu LIBR a_ 3 2 EDIT menu LIBR goes to menu EDIT editing of programme 2 directory 3 a_63 TEST1 HEAD menu EDIT editing of the header heated lid 99 C preheating of the lid on default name Test b 1 251C 1E STEP menu EDIT editing of programme step 1 Instruction Manual TRobot 10 2009 43 biometra a part of Analytik Jena temperature 95 00 C hold time 30 s c 1388 1E NSTP menu EDIT editing of the next step temperature 50 00 C hold time 30s g EEND menu Edit terminating of editing and return to menu LIBR lt CR gt terminating sign of the command block Return to the main menu If the command interpreter should return to the main menu the command has to start with The colon has always to combine with a command code Example request of the system status a lt CR gt 6 3 Synchronous transferred data Synchronous data are used for the control of the activity of a block They can be sent serially in an adjustable gap of 1 to 15 seconds The start and stop of the synchronous data transfer can be controlled with the command INTV in the menu BSET During the initialisation the release is deactivated After the activation of the synchronous data transfer the cycler sends periodically every second data beginning with
32. ber and press button En P in the Thermocycler Manager Software main screen see chapter 5 3 28 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena Program lo in Directo D Name ASA View pgm The programming screen opens see chapter 5 4 1 By the functions Delete step Insert step and new program steps can be inserted or existing program steps deleted To modify a step activate it in the programming screen and then change desired parameter in the input form Step Block Loop number temperature Name Goto number E fed ve fo nf af eh E 5 4 3 Load program To load a program stored on the computer press button Ge The program will be opened in the programming screen and can be edited as described in chapter 5 4 2 5 4 4 Save program Enter the parameters for all the required steps of the PCR program and then either click on the button eo or the button ae to save the program on the computer Define the name and location of the program and then click on lt save gt Speichern unter KE Speichern IC Temperature_Programs den TC m EZ Dateiname az Dateityp Text Files txt M Abbrechen E Save az text Press the button to export protocols as text files These may be opened and printed out with the Windows Editor Instruction Manual TRobot 10 2009 29 biometra a part of Analytik Jena Note In each case the program is saved to the computer harddisk See
33. biometra a part of Analytik Jena Robot Thermocycler Instruction Manual Model TRobot 96 TRobot 384 A I Warning Please read these instructions carefully before using this Ver 09 10 Order No 050 991 050 992 A apparatus Biometra GmbH Rudolf Wissell Str 30 D 37079 G ttingen Tel 49 0 551 50 68 6 0 Fax 49 0 551 50 68 6 66 email Info biometra com Internet http www biometra com Service Department Rudolf Wissell Strasse 14 16 D 37079 G ttingen Tel 49 0 551 50 68 6 10 or 12 Fax 49 0 551 50 68 6 11 email Service biometra com biometra a part of Analytik Jena This document describes the state at the time of publishing It needs not necessarily agree with future versions Subject to change 2 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena beer 6 1 1 Feld OW FAD NIC AU ON E 6 1 2 Special Features ccccecc cece escceeeceeeeeeceeeneeeneeeneeeeeseeeeeeeseeeeeegeesnetseeseeeseeeneeeaseeaeeegeees 6 1 2 1 Motorized heated Misere een ee ae ee a ee 6 1 2 2 Reliable uniform cycling nenn nano nenne nnnennnen nennen 6 1 2 3 SPAT OU GIN EE 6 1 2 4 VIOLOFIZEA DIALS MOE nannten 6 1 2 5 Communication with the TRobbot cccccecccsececeeeeeeeeseeeseeeeseeeceeetseeseeeaeeseeees 7 1 2 6 Internal Memory cccccccccceeccsecceeccueceuecaeeceecseecaeeseeecuecauecauesauesaeeseeeceeeeuessuesagees T 1 3 lee eeler EE 8 1 4 Rei Te
34. commands of the master become lost 6 7 2 Receiving Buffer overflow from the Cycler If the master sends more signs in one transmission block than the receiving buffer can store the cycler reacts with an error message As soon as the cycler receives a lt CR gt Carriage Return the receiving buffer will delete and the sent data will be lost 6 7 3 Checksum Error If the cycler recognises a checksum error a repeat transmission will be demanded from the master with an The same occurs for a checksum error recognised by the master It also demands a repeat of the last transmission from the cycler with an If this does not occur in the space of a reaction time especially after the faulty receiving of anew command the master has no access to the transmission The reaction time can be set in PROT WATM in 64ms steps Note If a fault message has been received it must be checked if the message begins with 1000 Then it is a reboot of a cycler and the check process is deactivated 6 7 4 Software Error If there is an error in the cycler software the safety programme SetError will be started After the registration of a software error the block stops an error message will be sent to the master and the cycler is inactivated After this the cycler must be started by switching off the cycler or the command 4 see chapter 6 5 4 The analyses of the error is checked by Biometra 6 7 5 Restart of the communication Se
35. d Thermocycler Both units are connected with a power cable 9 pin and a controller cable 37 pin Cables and plugs are shown in Figure 3 Both cables have a length of 3m 16 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena power cable controller cable 9 pin 37 pin Figure 3 Cable and plugs for connection of TRobot Thermocycler and controller unit e Connections on the back of the Thermocycler module are shown in figure 5 see chapter 4 2 e The plugs fit only the correct ports Incorrect connection is avoided Before connecting the unit to the power source please ensure that the voltage selector at the back side of the controller is set to the correct voltage Vor Inbetriebnahme unbedingt berpr fen ob der Wahlschalter fur die Netzspannung am Controller mit der rtlichen Betriebsspannung bereinstimmt Danger of electric shock Unplug the controller mains cable and the TRobot power and controller cable see Figures 3 and 5 before you open the Thermocycler or controller Gefahr eines elektrischen Schlages Vor dem Offnen des TRobot oder Controllergehauses unbedingt den Netzstecker vom Controller und das Controllerkabel und Netzkabel vom TRobot abziehen Instruction Manual TRobot 10 2009 17 biometra a part of Analytik Jena 4 Operating elements 4 1 The TRobot front view sealing around block heated lid 96 well silver block front locking unit plate lifter plate lifter
36. d der EC low voltage directive 73 23 EEC and the EG Richtlinie Uber die elektromagnetische Vertraglichkeit 89 336 EWG entsprechen EC directive about the electromagnetic compatibility 89 336 EEC Dr Jurgen Otte Quality Manager Instruction Manual TRobot 10 2009 69 biometra a part of Analytik Jena 14 Warranty This Biometra instrument has been carefully build inspected and quality controlled before dispatch Hereby Biometra warrants that this instrument conforms to the specifications given in this manual This warranty covers defects in materials or workmanship for 24 months as described under the following conditions This warranty is valid for 24 month from date of shipment to the customer from Biometra or an authorised distributor This warranty will not be extended to a third party without a written agreement of Biometra This warranty covers only the instrument and all original accessories delivered with the instrument This warranty is valid only if the instrument is operated as described in the manual Biometra will repair or replace each part which is returned and found to be defective This warranty does not apply to wear from normal use failure to follow operating instructions negligence or to parts altered or abused 70 Instruction Manual TRobot 10 2009 15 Subject Index Autorestart 60 Block Info 26 Connect Thermocyc ler 25 Declaration of Conformity 69 Decontamination Certificate 66 Direct command 36 Director
37. dioactive substances Please contact your responsible person for details Use of genetically change organism or parts of those Please contact your responsible person for details Instruction Manual TRobot 10 2009 67 biometra a part of Analytik Jena 12 Note for the disposal of electric electronic waste Note for disposal of electric electronic waste Hinweis fur die Entsorgung von Elektroaltgeraten du traitement des dechets des appareils lectrique lectronique a This symbol the crossed out wheelie bin means that this product should be brought to the return and or separate systems available to end users according to yours country regulations when this product has reached the end of its lifetime Renseignement For details please contact your local distributor This symbol applies only to the countries within the EEA EEA European Economics Area comprising all EU members plus Norway Iceland and Liechtenstein Dieses Symbol die durchgestrichene Abfalltonne bedeutet dass dieses Produkt von der Firma Biometra fur eine kostenlose Entsorgung zuruckgenommen wird Dies gilt nur fur Gerate die innerhalb Deutschlands gekauft worden sind Kontaktieren Sie fur die Entsorgung bitte die Biometra Service Abteilung AuBerhalb Deutschlands wenden Sie sich bitte an den lokalen Handler Dieses Symbol gilt nur in Staaten des EWR EWR Europaischer Wirtschaftsraum umfasst die EU Mitgliedsstaaten sowie Norwegen Isl
38. e chapter 6 5 3 and chapter 6 5 4 6 8 Special Commands 6 8 1 Commands to operate the Automated Lid The lid can be opened and closed only if the block is inactive or paused Use the commands OPEN and CLOS in menu BLCK After the cycler receives one of the two commands the cycler sends the reply to the command and moves the lid The successful opening and closing of the automated lid will not be communicated to the master The status of the lid can be checked with the command HSTT in menu BLCK If the lid is already in the position of the received command the reply is a corresponding error message to the master If the command STRT in menu BLCK program start follows the CLOS command the start of the program is delayed until the lid is closed The following errors result in a spontaneous error message by the cycler e Release of the safety switch e A defined time after the action the lid has not moved from his start position e After a period of waiting the lit did not reach his target position e Both end switches are released 48 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena e Emergency switch off of the control logic 6 9 Structure of Menus and Description of Commands 6 9 1 HOLD j CONT k TEMP I o Overview of the Structure of Menus System Main Menu SSTT a BLCK b LIBR c INFO d PROT f ALRM j Library LIBR c EDIT a CLRP b COPY c NPRG d Protocol PR
39. e profile ThermocyclerManager xls RE P IC H 1 Run protocol of program CYCLIST Lpen new source file 36 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena To open a file containing temperature data click button Open new source file and select the file to open The Excel file is divided into two sheets a Log Data b Log Curve a The sheet Log Data in lines 1 4 shows run data like the protocol name used type of Thermocycler and date From line 5 the data for the programmed target temperature the measured block temperature and lid temperature listed b In the sheet Log Curve the temperature data is displayed graphically Terperatcre o progremi C Temperature Run protocol of program CYCL TST Dir 0 Prog 0 e SE H on block 2 of T3000 S N 0000000 10 34 12 23 Aug 2004 a We L Run time h m s es N A BEE o o BEE BEE BEE oo The function Zoom allows the customized the display of temperature curves Click on button Zoom A new window opens where you can change the corresponding parameters to optimize the display Instruction Manual TRobot 10 2009 37 38 Yin Time me Wax Time Unni gt Il o s 0 O60 p biometra a part of Analytik Jena BE xi a Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 6 Serial Communication Protocol The following chapters describe all commands fo
40. epnr DD HHu step number of auto restart 0 99 if O then direkt mode was active blocknr D HHHHu block number 1 blocktype D HHHHu block type 0 96 2 384 blocktyps C CC C CCC C CC C CCC list of block types 0 96 2 384 Instruction Manual TRobot 10 2009 53 biometra a part of Analytik Jena bistatus BBBBBBBB HHHHu block status O FFFF BBBBBBBB low byte bit O block is running bit 1 controller or cooler error bit 2 plateau bit 3 ramp bit 4 autorestart bit 5 cooling 1 heating 0 bit 6 lid preheating bit 7 pause high byte bit O headed lid error bit 1 cooler error bitemp DD D HHHHs set block temperature 3 00 99 90 bltempa DDD D HHHHs actual block temperature 7 0 101 7 checktype B B protocol check 0 no check 1 check company CCCCCCCC CCCCCCCC Iname of company Biometra cooltempa DD D HHHHs actual cooler temperature 2 30 70 00 ctrlnr D HHHHu controller number 1 3 cyclcount DD HHHHu cycle counter 1 100 cyclertype CCCCCCCCC CCCCCCCCCC cycler type TRobot dirname CCCCCCCC CCCCCCCC directory name A 2Z lt gt amp dirnr D HHHHu directory number U 9 FF if direct mode is active echoflag B B echo on off 0 off 1 on gradient DD D HHHHs set temperature gradient 0 40 0 if gradient possible 0 if gradient not possible handsflag B B hardware handshake on off 0 off 1 on htime DD DD DD HHHHu s
41. essure and can cause bruises when operated improperly Biometra strongly recommends to use a 9V battery to open and close the lid see chapter 7 4 Beim SchlieBen des Deckels niemals die Hande in den Thermocycler halten Durch die Kraft des Deckels besteht bei unsachgemaBer Handhabung die Gefahr von Quetschungen Biometra empfiehlt den Heizdeckel mittels einer 9V Batterie zu ffnen und zu schlieBen siehe Abschnitt 7 4 4 4 Adjust TRobot lid to zero position The TRobot lid has to be initially adjusted to the specific type of microplates that will be used The reason is that plates from different suppliers differ in height which has a strong impact on the lid pressure 1 Turn the knob on the TRobot lid to 0 2 Remove the white cap on top of the knob unscrew two screws 3 Open the TRobot lid either by using a battery see chapter 7 4 or with the Thermocycler Manager software 4 Put the desired microplate on the TRobot block complete with sealing 5 Close the TRobot lid either by using a battery or with the Thermocycler Manager software 6 Release the internal heated lid by screwing the hexagon socket counter clockwise ge PN Hexagonal socket Release Fix Down ap Metal rin g Hexagonal socket Metal ring fix and release lid alter lid height 20 Instruction Manual TRobot 10 2009 7 4 5 Setting lid pressure biometra a part of Analytik Jena
42. et holding time s or m 00h00m01s 08h59m59s short format 09h00m 99h59m hh mm ss long format 0 pause htimea DD DD DD HHHHu holding time sor m 00h00mO1s 08h59m59s up till now 09h00m 99h59m interval DD HHHHu synchronous data 1 15 interval s 0 life sign no parameter gt stop lidtemp DDD HHHHu set lid temperature 30 99 0 no heating 54 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena lidstatus BBBBBBBB HHHHu heated lid status 0 FFFF BBBBBBBB low byte bit O lid is heated bit 1 lid too hot bit 2 lid too often on bit 3 lid is fast heated high byte bit O lid is open bit 1 lid is closed bit 2 HW error 1 bit 3 HW error 2 bit 4 time out of engine bit 5 safety switches on lidtempa DDD HHHHu actual lid temperature 10 00 139 00 lintempfac DDDDD HHHHs up to 12 factors for calculation 16000 16000 DDDDD HHHHs of line temperatures separated by commas loop DD HHHHu back cycle 1 actual step number step number 0 no loop preheating B B set preheating lid 0 no preheating 1 preheating progname CCCCCCCC CCCCCCCC program name A 2Z lt gt amp prognr DD HHHHu program number 0 99 FF if direct mode is active protver DD DD DD DD DD DD DD DD protocol version 00 00 00 00 99 99 99 99 remtime DDDDD HHHHU remaining time of active 0 59940 min
43. firm your selection with lt yes gt Thermocycler Manager N Program 10 in Direcory 9 delete When the program is deleted a new window opens showing the following message Thermocycler Manager u Direcory 9 program 10 deleted 5 5 5 Delete directory In the window for memory management see chapter 5 5 enter the number of the directory that you would like to delete Director m Press button Melete directo and in the next window enter the serial number of the Thermocycler Delete directory For DELETE ALL PROGRAMS of directory number 9 Cancel type the senal number of cycler Note The serial number of the instrument is written on the type label Press button Uk In the next window press lt yes gt to confirm the deletion of the directory Instruction Manual TRobot 10 2009 33 biometra a part of Analytik Jena Thermocycler Manager AN All programs of direcory 9 delete When the directory is deleted a new window opens showing the following message Thermocycler Manager A In directory 9 are all 1 programs deleted 5 5 6 Save all programsto PC The Thermocycler Manager Software offers the function to save all programs located in the memory ofa Thermocycler to PC Press button BEE EES EE in the window for memory management see chapter 5 5 In the next window define the path where to programs are going to be saved to and confirm your selection with lt save gt al Holger
44. follows the command code and one or more parameters Several parameters are separated by a An optional parameter between two other parameters could be left out by setting a separator Optional parameter at the end may be left out totally For an example a command block with multiple commands see chapter 6 2 5 Command Codes Basically you have to distinguish between long and short commands The current version implements only short commands The following description also shows the long commands The list of all command codes is given in chapter 6 9 2 short Commands The command code of short commands is defined by a single letter The command letter may be used for different commands in different submenus but each command may by used once within the same submenu see chapter 6 9 2 Note When short commands are used the parameters are also transferred in a compact format see chapter 6 10 Long Commands yet to be implemented The command codes of long commands are constructed of four letters The letter combination is used in such a way that it draws conclusions from the commands Each letter combination exists only once see chapter 6 9 2 Note When using of long commands the parameters are transferred in a simple readable format see chapter 6 10 Shifting between Short and Long Commands yet to be implemented After the Initialisation the communication works with short commands If the cycler receives from the master
45. ilm Heat Sealing Film Heat Sealing Film Aluminium 96 well aluminium plate 384 well aluminium plate Nop mat 96 well Nop mat 384 well Quantity 1000 pcs 1000 pcs 125 pcs 125 pcs 50 pcs 25 pcs 25 pcs 25 pcs 50 pcs 50 pcs 50 pcs 100 pcs 100 pcs 10 pcs 1 pcs 1 pcs 20 pcs 20 pcs 63 biometra a part of Analytik Jena 10 Service Should you have any problems with this unit please contact our service department or your local Biometra dealer Biometra GmbH Service Department Rudolf Wissell Stra e 14 16 D 37079 G ttingen Phone 49 0 5 51 50 68 6 10 or 12 Fax 49 0 551 50 686 11 e mail Service biometra com If you would like to send the unit back to us please read the following return instructions in chapter 10 1 10 1 Instructions for return shipment In case of an instrument failure that cannot be fixed by the procedures described in section 7 please proceed as follows e Return only defective devices For technical problems which are not definitively recognisable as device faults please contact the Technical Service Department at Biometra Tel 49 551 50881 10 12 Fax 49 551 50881 11 e mail service biometra com e Please contact our service department for providing a return authorization number RAN This number has to be applied clearly visible to the outer box Returns without the RAN will be not be accepted e Important Carefully clean all parts of the i
46. ing standard features the TRobot can provide extensive status messages that allow monitoring of all instrument parameters A software tool for directly operating the TRobot from a Personal Computer is also available 1 2 6 Internal memory The TRobot provides an internal memory organized in 10 subdirectories Programs can be started either by downloading the protocol prior to starting this program Alternatively an existing program can be started from the internal memory of the TRobot Instruction Manual TRobot 10 2009 T biometra a part of Analytik Jena 1 3 Technical specifications TRobot 96 TRobot 384 motorized heated lid motorized heated lid Capacity 96 well microplate 384 well microplate Block 96 well gold plated silver block 384 well aluminium block special coating Heated Lid BE Temperature range 30 99 C 30 99 C adjustable in 15 increments adjustable in 15 increments Thermocylcer dimensions EEE ee 7 kg 37 pin controller 37 pin controller 10 pin power 10 pin power Controller ee Maximum power 350 Waitt 350 Waitt consumption 37 pin controller 37 pin controller 10 pin power 10 pin power serial RS232 serial RS 232 Dimensions W x L x H 190 x 440 x 125 mm 190 x 440 x 125 mm Weight 5 9 kg 5 9 kg 8 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 1 4 Legal Notes 1 4 1 PCR License Legal Disclaimer Purchase of a Biometra Thermocycler conveys a
47. inr c 1d temperature scb temp t 5f1C current scpeltcur i 4f1A and pulse scpuls p 4x See chapter 6 2 4 200 201 202 203 204 205 206 no block error bctip no error unidentified error bctip unidentified block error emergency off bctip error detected by hardware peltier contact bctip missing peltier contact temp fault bctip block temperature out of range cooling current low bctip set temperature cannot be reached heating current low bctip set temperature cannot be reached Instruction Manual TRobot 10 2009 57 6 11 4 6 11 5 6 11 6 6 11 7 58 207 208 209 210 211 biometra a part of Analytik Jena lid too hot bctip heated lid temperature out of range cooler fault bctip cooler temperature out of range opening not possible bctip lid cannot be opened automatically closing not possible bctip lid cannot be closed motor disconnected bctip both endposition switches are active Error Group 3xx Block Using Errors 300 301 302 303 304 305 306 307 308 309 310 pgm finished or paused regular program end or pause no error start not possible block is already active block off block is already off blocks 1 1d wrong block number lid is open lid is already open lid is closed lid is already close lid is not in endposition lid not ready start not possible block is active not possible block is active wrong b
48. l TRobot 10 2009 biometra a part of Analytik Jena d ITOR timeoutlen timeout 0 disables time out of the receiving buffer timeoutlen e HWHS handsflag handsha 0 without handshake default handsflag K length gt 0 enables time out of the receiving buffer Parameter is the initialisation value of the time out counter values between 1 and 255 64ms Without parameter sends time out length e 1 hardware handshake type without parameter sends handshake type 6 10 Formats of the parameters D decimal B bit C character sign nothing HHHH flexible format max 2 bytes converted in 1 2 3 or 4 ASCII hex characters CO 9 A F a Ti HHHHHH the same but max 3 bytes possible value 100d The real value is multiplied by 100 before converted to hex special time format if value lt 9 hour then seconds convert to hex else then minutes converted to hex and add up 8000h u unsigned s signed highest bit determines the sign 0 plus 1 minus Parameter long format short format description range blocks D HHHHu number of blocks cet lines DD HHHHu number of lines 1 12 loops DD HHHHu number of back cycles 1 99 0 no loop sensors D HHHHu number of sensors 1 3 steps DD HHHHu steps 0 99 alarmil B B acoustic signal 0 no alarm 1 alarm acttime HHHHu HHHHu cyclical time counter 64ms 0 FFFF aurest
49. lid exerts significant pressure and can cause bruises when operated improperly Der TRobot ist fur die Integration in automatisierte Anlagen konzipiert Wahrend des Betriebs sollte fur Anwender kein Zugang zum Ger t m glich sein Das Ger t ist mit einem motorisierten Heizdeckel ausger stet der beim Schlie en erhebliche Kraft aus bt Beim Schlie en des Deckels niemals die H nde in den Thermocycler halten Durch die Kraft des Deckels besteht bei unsachgem er Handhabung die Gefahr von Quetschungen Do not use alcohol e g methanol ethanol organic solvents or abrasives to clean the instrument Verwenden Sie keine Alkohole Methanol Ethanol organische L sungsmittel oder Scheuermittel zur Reinigung des Instruments For transports always use the original Biometra box Verwenden Sie zum Transport des Ger tes ausschliesslich die Biometra Originalverpackung Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 3 Installation 3 1 Content of delivery 1 TRobot Thermocycler 2 Controller TRobot 3 Manual 4 Thermocycler Manager Software Please keep the original packaging material for return shipment in case of servicing The TRobot shipping box provides a specially developed system for contact free transport of this electronic device 3 2 Unpack and Check Unpack and carefully examine the instrument Report any damage to Biometra Do not attempt to operate this device if physical damage is present
50. location of the Thermocycler If the currently viewed program is stored at the highest memory location the software starts to search at the beginning of the memory and the program at the first used memory location will be displayed b Find active program Search for the currently used program c Find the program with name Search for a program by name d Begin to search in directory number Finds the program that is stored at the first used memory location in the specified directory 5 5 2 View program Press button Vu program in the memory management window see chapter 5 5 to enter the programming screen see chapter 5 4 In the programming screen programs can be viewed and edited 5 5 3 Copy progam The function Lopy Program allows to copy programs After activation the following windows opens Copy program Copy Program i mo Director g Cancel ho Program fo in Directory g In the window you can specify which program where to be copied Press Uk to confirm your selection The program then will automatically be copied to the chosen memory location Note Existing programs will be overwritten 32 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 5 5 4 Delete program In the window for memory management see chapter 5 5 enter the location of the program that you would like to delete Program fia in Directory H Press button Hegle program and in the next window con
51. lock type wrong block type no in tube sensor no intube sensor connected pgm not paused program isn t paused Error Group 4xx Memory Error 401 402 403 404 405 406 name deleted Error in directory name pg 1d 2d and the following empty Error in program memory 3d pgm s empty Error in program memory no steps program without any step data error block 1d stopped error in active program after autorestart checksum error checksum error of software Error Group 5xx Input Syntax Error 501 502 503 504 505 506 507 508 invalid command command not available in this menu missing command command expected missing separator separator expected separator error wrong use of separator missing apostrophe apostrophe expected convert error conversion error in parameter missing parameter parameter expected wrong parameter wrong parameter Error Group 6xx Buffer Error 601 602 603 604 611 612 613 621 622 rx write denied writing into the receiving buffer not possible rx read denied reading from the receiving buffer not possible overflow rx overflow of the receiving buffer rx time out time out of the receiving buffer tx write denied writing into transmit buffer O not possible tx read denied reading from transmit buffer O not possible overflow tx overflow of the transmit buffer O syO write denied writing into transmitting buffer
52. metra recommends that you first read these instructions carefully This operation instruction is part of the product and should be kept over the full life time of the instrument It should also be forwarded to subsequent owners and users Make sure that additions and updates are inserted into the operation instructions Instruction Manual TRobot 10 2009 9 biometra a part of Analytik Jena 2 Safety and Warning Notices 2 1 Definition of Symbols Symbol EPP Definition Caution Refer to instruction manual Achtung Beachten Sie die Hinweise im Handbuch Danger High voltage Gefahr Hochspannung Fragile Zerbrechlich Danger Hot surface Gefahr Heisse Oberflache Please read this manual carefully before starting operation of the TRobot Thermocycler The TRobot Thermocycler is intended for sample incubation at varying temperatures e General safety precautions for laboratory work must be observed when working with the TRobot Thermocycler e The TRobot does not produce a sound power level that could be hazardous for the user A A A A The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Das schnelle Heizen des Thermoblocks kann dazu fuhren dass Flussigkeiten explosingsartig verkochen Verwenden Sie daher immer eine Schutzbrille w hrend des Betriebs Schliessen Sie vor dem Start eines Programms den Deckel des Ger tes Rapid heating
53. mon management Figure 7 Thermocycler Manager Software main screen Since it is not yet a Thermocycler connected to the software the status messages contain blue question marks and most of the buttons appear greyed out 5 3 1 Network settings Click on button Settings to open the menu for network settings Instruction Manual TRobot 10 2009 23 biometra a part of Analytik Jena C Come Com Comd C Com5 Comb Com Comd Com3 Comid Com Coml Default C Comi3 Coml4 Comi5 Comi6 Timeout vw Onja Record temperature profile Temperature data conversion l Select number 7 1 0 Save data in Vase f 10 File Convert Figure 8 Network settings screen Activate the radio button for the serial port Com Port the Thermocycler that you would like to address is connected to 5 3 2 Record temperature profile The Thermocycler Manger Software can save a temperature profile for each run Each second the set and the actual temperature of the block and the actual temperature of the lid are recorded The data later can be analysed with a Microsoft Excel file specially configured by Biometra Temperature profile ThermocyclerManager xls To record temperature data activate the check box M Save datain see figure 8 chapter 5 3 1 The software either saves the files in a File preset folder or you may also set another path Click on the button Anew window opens where the n
54. nstrument of biologically dangerous chemical or radioactive contaminants If an instrument is contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible losses resulting from insufficient decontamination of the device e Please prepare written confirmation that the device is free from biologically dangerous and radioactive contaminants The declaration of decontamination see section 11 must be attached to the outside of the packaging e Use the original packing material If not available contact Biometra or your local distributor e Label the outside of the box with CAUTION SENSITIVE ELECTRONIC INSTRUMENT e Please enclose a note which contains the following a Senders name and address b Name of a contact person for further inquiries with telephone number c Description of the fault which also reveals during which procedures the fault occurred if possible 64 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 10 2 Packing of the TRobot Thermocycler Biometra uses an extra designed packaging system where the instrument is mounted in between two tearproof foils The TRobot Thermocycler and controller are put onto the lower inlet and is fixed in between the foils by pressing the upper inlet down Note The instruments are only protected from transport damage if the packing instructions are followed and if they are mounted in between
55. of the thermoblock can cause liquids to boil explosively Always wear safety goggles and gloves during operation Close the lid before starting a program Das schnelle Erhitzen von Fl ssigkeiten kann zu plotzlichem berkochen f hren Tragen Sie daher immer Schutzbrille und Handschuhe w hrend des Betriebs Schliessen Sie den Deckel bevor Sie ein Programm starten Do not heat samples without having the lid locked securely Stellen Sie vor Programmstart sicher dass der Deckel sicher geschlossen ist Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena Be aware that samples are reaching high temperatures Do not touch or open hot tubes or microplates because hot liquid may quickly spill out Die Proben k nnen hohe Temperaturen erreichen Fassen Sie heisse Probengef e oder platten nicht an und offen Sie diese nicht andernfalls kann kochende Flussigkeit herausspritzen Do not touch the heated lid Fassen Sie nicht an den Heizdeckel Use only suited plastic ware in the TRobot thermocycler Tubes and plates must show good fit when placed in the thermoblock Only use tubes that are suited for high temperatures tight lids Benutzen Sie nur Platten und Gef e die f r hohe Temperaturen bis 100 C geeignet sind die gut in den Thermoblock passen kein Wackeln und deren Deckel dicht schliessen The TRobot Thermocycler contains no user serviceable parts Do not open the housing instrument Service and
56. ommunication protocols Example 000_0 0 1 0 lt CR gt During the power up the cycler checks its own memory content If an error is found during the check a further message block with the corresponding error message will be added to the initialisation message Example During the save test an uncompleted program is found because the cycler has been switch off during the editing of this program The uncompleted program is removed from the memory and the following message is shown I403 lt CR gt program empty If the cycler software comes across an error in the software the following error message is shown a new power up and the initialisation message 1002 Error code1 code2 code3 code4 code5_code6_code7 lt CR gt blank code HEX code 4 digit This special message is the same in the long and short format The analysis of the code will be done by Biometra Spontaneous messages which are generated between the power up of the cycler and the first communication with the master see chapter 6 2 4 are also temporarily stored 6 5 2 Activation of the Remote Control 6 9 Sending and a command activates the remote control of the Cycler 6 5 3 Re Initialisation of the Communication The command lt ESC gt lt ESC gt 2 ASCII 1B without any other sign re transfers the communication to its starting point All sending and receiving buffers are reset all settings of the communication are re transferred to the setting of
57. or 384 well microplates 050 231 The 384 well block is coated with a special alloy which supports easy removal of plates by the motorized lifter 1 2 3 Small footprint To produce a compact instrument the TRobot block module has been separated from the controller unit Thus the controller can be placed outside the workstation By switching the Thermocycler chassis the airflow direction can be inverted front to back or vice versa This minimizes contamination due to air flowing towards a liquid handler or other components 1 2 4 Motorized plate lifter Removing plates from the block has been one of the major obstacles to Thermocycler automation Due to the tight fit air between the block and the plate is displaced during cycling 6 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena causing microplates to stick This makes it difficult for the plates to be removed by a robotic arm To solve this problem the TRobot is equipped with four levers that lift the plate from the block as the lid opens Once lifted the plate can be easily removed by a robotic arm The levers then return to their initial position allowing a new plate to be positioned 1 2 5 Communication with the TRobot All features of the TRobot are controlled via its serial port A detailed communication protocol is available see chapter 6 Integration in existing software environments of individual robotic stations is very easy In addition to controll
58. or text plant text error message English btip only in error group 2xx see chapter 6 11 3 CRC16 check code optional lt CR gt ASCII Carriage Return OD After a long command the error message returns in the long form otherwise in the short form The reply code is identical in both forms The error message consists the reply code command code with a capital letter blank error identifier as well as in the long form the error text separated by a blank If the last command was sent in the long form the error message is in the long form Otherwise it is in the short form The error message consists of the error identifier as well as in the long form the error text separated by a blank from the error identifier In Error group 2xx see chapter 6 11 3 the parameter btip block number b temperature t current i and pulse p will be transferred Example 1004 lt CR gt short form 1004 cooler too hot lt CR gt long form 6 2 5 Menu Structure All commands are sorted in a menu structure with a main menu and several submenus see chapter 6 9 1 Within a menu only the commands of this menu are available The command codes in the short form are termed alphabetically a bc The short command code b has different meanings in different submenus After the power up the command interpreter is in the main menu To move from one menu to a submenu a command has to be sent All commands are explained in the command list see ch
59. orized lid can be operated with a 9V block battery This option can be used in case of power failure to remove the samples For operating the lid a battery is contacted to the socket on the rear side of the Thermocycler see chapter 4 2 Open Close Deutsch oo Only use a 9V block battery to open Verwenden Sie ausschlie lich eine the TRobot lid manually 9V Blockbatterie um den TRobot Deckel manuell zu ffnen 7 5 Recommendations for the use of 384 well Thermocyclers a Use the recommended 384 well plates and sealing materials To prevent sample evaporation by insufficient sealing please use 384 well plates and sealings tested and recommended by Biometra A list of plates and sealing is available at www biometra com gt thermocyclers gt plasticware b Reduce the temperature of the heated lid 60 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena Due to the fact that the heated lid is very close to the reaction volume with a 384 well plate compared to 0 2 ml vessels the lid temperature can have a significant impact on the effective incubation temperature For reaction volumes less than 10 ul the lid temperature is recommended to be set at 95 C When using a reaction volume of 10 ul or more set lid temperature at 85 C Even at these lowered lid temperatures possible condensation will not occur as problem due to a mixture of physical factors like dew point salt concentration temperature difference air p
60. r serial communication with the TRobot This serial documentation protocol is the basis for integrating the TRobot in automized workstations Since workstations from different manufacturers have different software systems the TRobot has to be integrated indidvidually For integration please contact the manufacturer of your workstation 6 1 General Information The definition describes the communication between a Biometra TRobot and a controlling device the master You have to distinguish between asynchronous communication and synchronous data With the asynchronous communication the master transfers single commands to control the cycler The synchronous data is used for the control of the thermoblock activity If this mode is online the cycler transfers in periodic cycles data about the operating conditions of the thermoblock 6 2 Asynchronous Communication 6 2 1 General Commands and replies The controlling device transfers one or more commands to the cycler in one block The command interpreter works through the commands in chronological order The cycler sends conformation to the master on completion of each task Each data block terminates with lt CR gt ASCII Carriage Return Each Command Induces a Reply Exceptions If the cycler notices an error during the processing of a command all following commands in the same data block will be ignored and the master receives no reply from the cycler In gener
61. repair may only be carried out by the Biometra Service department or otherwise qualified technical personal Der TRobot Thermocycler beinhaltet keine Teile die vom Anwender gewartet oder repariert werden k nnen Offnen Sie nicht das Geh use des Thermocyclers Lassen Sie Service und Reparaturen von der Biometra Service Zentrale oder von qualifiziertem Fachpersonal durchf hren Do not use the instrument when damages of the housing block cable or other parts are visible Benutzen Sie den TRobot Thermocycler nicht wenn Besch digungen am Bock Geh use Kabel oder an anderen Teilen erkennbar sind Prior to connecting the unit to the power source please ensure that the voltage selector at the backside of the controller is set to the required voltage and make sure that the appropriate fuses are inserted in the fuse holder see chapter 3 3 Stellen Sie vor dem Anschalten sicher dass die Einstellung des Spannungswahlschalters auf der R ckseite des Controllers mit der Netzspannung bereinstimmt und die passenden Sicherungen im Sicherungshalter eingesetzt sind siehe Abschnitt 3 3 Instruction Manual TRobot 10 2009 11 A A A A A biometra a part of Analytik Jena Make sure that the main supply voltage is in accordance with the label above the power connection Die Netzspannung mu mit dem Wert auf dem Typenschild ber dem Netzanschluss bereinstimmen Unplug the power cable before you open the TRobot The
62. ressure within the tube and reaction volume It is strongly recommend to reduce the lid temperature according to the reaction volume Otherwise at conventional lid temperature settings sample volumes or amplification yields might be reduced Instruction Manual TRobot 10 2009 61 biometra a part of Analytik Jena 8 Maintenance and repair 8 1 Cleaning and Maintenance The TRobot was built to operate for a long time without the need for periodical maintenance Nevertheless occasionally cleaning of the air inlet may be necessary to maintain the efficiency of the Thermocycler Insufficient airflow may lead to reduced heating and cooling rates The inlet for the airflow is located at the front side of the instrument See chapter 4 Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dust can be removed easily from the inlet with a conventional vacuum cleaner Additionally the Thermocycler housing may be cleaned from time to time with a smooth cotton cloth Do not use strong detergents or organic solvents for cleaning Never treat the block with abrasive agents Important Appropriate safety regulations must be observed when working with infectious or pathogenic material 8 2 Service and repair The TRobot Thermocycler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried ou
63. ring interval of synchronous syncaata sync data and starts output of data interval 0 Sends sign of repeated data life without data each second interval 0 Sends data every interval and if necessary sign of life each second in which no seconds data are sent Without parameter Stops output of c SIM tempfor tmp Sets or sends temperature format of synchronous data A tempform m format middle temperature Without parameter query for svnchro d BDAT block Sends block characteristics Number of sensors lines sensors characte spreading factor conversion factor 0 if gradient not lines ristics possible spreadfac lintemofac or 6 9 6 Menu Info INFO co mnemon paramete description reply a COMP compan company b CTYP cycler cyclertype type CG ISVER software sversion version d SERN serial Software serial number ssernr number e PVER protocol protver version f BIPS block string of possible block types blocktyps types g BLKS number blocks of blocks 6 9 7 Menu Library LIBR co Imnemo paramet description reply a EDIT dirnr jedit gt SM EDIT Without parameters last used program With dirnr prognr prognr program parameters edit program prognr of directory dirnumber b CLRP dirnr clear Error if program is active dirnr prognr prognr program c COPY l dirnr copy Error if destination is active prognr progr
64. rmocycler Danger of electric shock Vor dem ffnen des Geh uses unbedingt den Netzstecker ziehen Gefahr eines elektrischen Schlages Make sure that the appliance connector and the plug of the supply cord are accessible so you can separate the instrument from the mains Stellen Sie sicher dass der Netzschalter und das Netzkabel gut zug nglich sind so dass das Ger t im Bedarfsfall von der Netzspannung getrennt werden kann Connect the TRobot Thermocycler to a grounded socket Schliessen Sie den TRobot Thermocycler an eine geerdete Steckdose an When only few samples are put in the block place additional tubes in the four corner positions This is to evenly distribute the lid pressure and prevents single tubes from excessive pressure Use of few tubes may result in damage of the tubes by excessive pressure Sofern nur wenige Proben behandelt werden bitte zus tzlich jeweils ein leeres Gef ss gleicher H he in die Eckpositionen des Blocks setzen Bei einer zu geringen Probenzahl im Block besteht die Gefahr dass Gef sse besch digt werden Appropriate safety regulations must be observed when working with infectious pathogenic or radioactive material Ask the responsible local safety inspector for details Wenn pathogene radioaktive oder infekti se Proben behandelt werden m ssen die einschl gigen Sicherheitsbestimmungen ber cksichtigt werden Fragen Sie den f r Ihren Standort zust ndigen Sicherheitsbeauftragen f r
65. rmocycler have to be controlled via user friendly software After cycling plates need to be easily removed for downstream processing These specifications were the starting point for the development of the new TRobot Thermocycler 1 2 Special Features Designed specifically for robotic systems Integrated cycler with external controller Proven peltier technology Unique motorized plate lifter Motorized heated lid Lid opening angle 110 Adjustable lid pressure Small footprint Available with 96 well silver or 384 well aluminium block Low power consumption Communication with PC via serial RS232 protocol Instrument provides extensive status report 1 2 1 Motorized heated lid The TRobot lid is powered by a two step electric motor First the lid closes quickly then the second mechanism carefully interlocks the lid at the front This ensures that a defined reproducible pressure is applied When the lid is closed the electric motor is uncoupled for a long life The amount of pressure exerted by the lid can be precisely set to one of 15 different positions 1 2 2 Reliable uniform cycling The TRobot Thermocycler is available in two different block formats Taking advantage of the high thermal conductance of silver the TRobot 96 achieves high ramping rates as well as excellent temperature uniformity The silver block is coated with gold to ensure lasting performance The TRobot 384 block is made of aluminium and achieves perfect fit f
66. sferred data 44 6 3 1 Data format of synchronously transferred data 44 6 3 2 Sending Only One TiNe an dese saneaiceunedwiGurnmnendwinermdereued 45 6 4 Setting of the Transterbrotocol nano nenn nano nnnnn anne nenn ennenen 45 6 5 COMMUNMIGAUON Start and Ee e u nenne 46 6 5 1 Power up of the cycler Connection start 46 6 5 2 Activation of the Remote Control 46 6 5 3 Re Initialisation of the Communication 46 6 5 4 Re Initialisation of the Cycler Software 00nnaannannnnnnennnnnnnnnennnnnsnrnnrnnrrnrrnenrnne 47 6 5 5 PEO Si EE 47 6 6 Setting of the Communication 47 6 6 1 Check DrocessChRCIp nenn nenn nnne nennen snnn nennen 47 6 6 2 HANSA AAT E a nero 47 6 6 3 EENEG 47 6 6 4 Transfer of Synchronous Data 47 6 6 5 Time out of the Receiving Butter 47 6 7 Complications during the Communication 48 6 7 1 Communicational Collisions ccccecce seen eeeeeeeeeteeeteeeteeceeeeeeeeeueeseeteeeteeeeeeenaes 48 6 7 2 Receiving Buffer overflow from the Cycler aannannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnrnnnennnne 48 6 7 3 SM CK U ET de E 48 6 7 4 SOHWArE EINOT E 48 6 7 5 Restart of the COMMUNICATION ccceccceceseeeceeeceeceeeeeeeceeeseeeseeeseeeneeeeeeeeeseeeees 48 6 8 See E e e ln ne E 48 6 8 1 GommandetooperatetfeAutomated gd 48 6 9 Structure of Menus and Description Of Commande 49 6 9 1 Overview of the Structure Of Menus n ennonnennennnnnnnnnnonnsnrorrsnrerrerrsrrsrrnrrererernne 49 Instruc
67. software without running block Lid error flag is cleared with clearing the heated lid status lidstatus reset cooler error flag b BSET __ iiblocksetup gt SMBSETO _ _ o HSTT heated lid Sends status of heated lid With O following error idstatus status flags are cleared emergency off of automated lid safety switch released motor time out The flags lid too hot and lid too often on are automatically cleared with starting the next program or reset of the cycler software e Soar Wm Sends once data in the same format as the syncdata ma once synchronous data once a lid open Opens lid doesn t send any success message The lid position can be questioned in lidstatus see HSST If the lid is already open then error TT lid close Closes lid doesn t send any success message The lid position can be questioned in lidstatus see HSST If the lid is already closed then error Ka rnr start block With parameter starts program prognr of directoryldirnr prognr dirnumber Without parameter starts the last used program Error message if this program isn t edited completely Fo ke block Only active block otherwise error message j Hoo pause pause Only active block otherwise error message active Only active block otherwise error message otherwise error message a u continue Continues the paused program switches off the present acoustic signal Only at pause otherwise error message T
68. t by the Biometra Service department or otherwise qualified technical personal The Service department offers Thermocycler maintenances and temperature verifications Biometra recommends an annual maintenance and a biannual temperature check for all Thermocyclers Please call the following phone number for detailed information 49 551 50881 10 12 8 3 Firmware update For instruction for firmware upgrade please contact the Biometra Service Department or your local distributor sales representative 8 4 Replacement of Spare Parts Only original spare parts mentioned in these operating instructions are allowed 62 Instruction Manual TRobot 10 2009 9 Accessories 9 1 Plasticware biometra a part of Analytik Jena Biometra offers a broad range of plasticware for the use in PCR The following table provides an overview for the product portfolio Cat no 050 310 050 320 050 254 050 255 050 225 050 232 050 213 050 253 050 231 050 240 050 237 050 256 050 257 050 236 050 194 050 694 050 251 050 252 Instruction Manual TRobot 10 2009 Description Single tubes 0 2 ml tubes with caps 0 5 ml tubes with caps Tube strips Strips 8 tubes and flat caps Strips 8 tubes and domed caps 48 well plates 48 well microplate 96 well plates 96 well skirted 96 well non skirted low profile 96 well non skirted 384 well plates HSQ 384 well skirted 384 well microplate Miscellaneous Silcone mat Adhesive f
69. t of Spare Hate 62 GCOS SS asc ste se ae tm we ses sie nenn un ec ein es ates eee ENAERE ern 63 9 1 Plasticware recent sauna einen een ER Eee 63 10 EENEG 64 10 1 Instructions for return ship Ment nenn nnnennnennn san snnnnnnn nennen 64 10 2 Packing of the TRobot Themmocvcer nenn anne ernennen 65 11 Equipment Decontamination Certificate euuruunnaannunnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 66 12 Note for the disposal of electric electronic waste uuuunnanennannnnnnnnnnnnennanennnnnnnnnnenn 68 13 Declaration of Conformity essen se setae suede nstoncancadsuicusmeteruvencnsemecssenssvecseussieutsesseaweevens 69 1 WV ABU In ar a dasseeveseteastedesmeen nese EEE UNE NEE SENSOR SEC EEENENEINS SEERNSERPEREEEUERERER 70 15 SUISSE TNO ee 71 Instruction Manual TRobot 10 2009 5 biometra a part of Analytik Jena 1 Introduction 1 1 Field of Applications The Biometra TRobot is a Thermocycler that is licensed for PCR applications research use only and intended to be used for performing polymerase chain reactions For integration in automated environments a Thermocycler has to meet very different needs than a bench top instrument Since space under the robotic arm is limited a Thermocycler needs a small footprint To ensure automated workflow the instrument must have a motorized lid that allows the robotic arm to access the block easily and that is strong enough to reliably seal microplates All features of the The
70. t using hardware handshake both CTS and RTS line of the communication cable must be wired a null modem cable is not suitable Handshake must be enabled at the Cycler with command HWHS in menu PROT and the controlling device 6 6 3 Quasi Echo Operation To check the quality of a connection the quasi echo operation can be used During the quasi echo operation the cycler repeats the transferred command string of the master During the active check the cycler attaches its check value to the received command The ECHO command can be activated in menu PROT 6 6 4 Transfer of Synchronous Data See chapter 6 3 Activating with the INTV command in menu BSET 6 6 5 Time out of the Receiving Buffer The serial communication can be controlled by the measurement of the receiving time Whenever the first sign arrives at the cycler the time out counter starts and stops when the lt CR gt reaches the cycler If the adjusted time has elapsed an error will be reported and the received data will be deleted The Time out time can be set in 64ms steps with the ITOR command in menu PROT Instruction Manual TRobot 10 2009 47 biometra a part of Analytik Jena 6 7 Complications during the Communication 6 7 1 Communicational Collisions The Master did not wait for all replies and sends new commands before the last message arrives from the cycler In this case it receives the error message 601 the cycler works out the previous commands and the last
71. that can be sent to the Biometra service by email This allows to some degree an advanced remote diagnosis in case of problems To create a Service Info File press Make a service fle Assign a file name and then select the location to save the file on your computer Instruction Manual TRobot 10 2009 35 biometra a part of Analytik Jena Speichern unter Speichern E Service da Ir E Biometra Service Info File Serial Number 1308276 Dateiname Biometra Service Info File Serial Number 13082 Dateityp Ten Files D tot sl Abbrechen Send the file by email to service biometra com 5 5 9 Direct command Biometra Thermocyclers can be directly controlled by serial commands Press button Direct command E to activate the Thermocycler Manager Software command line Enter a command and press button Send command Chapter 6 gives a comprehensive overview over all serial commands 5 5 10 Exit memory management window Exit Press to leave the window for memory management 5 6 Exit Thermocycler Manager Software Press button Exit 5 3 to leave the software in the Thermocycler Manager Software main screen see chapter 5 7 Analysis of temperature data For Microsoft Excel based analysis of temperature data the file Temperature profile _ ThermocyclerManager xls has to be installed on the computer The file allows the reading and the graphic representation of temperature data Open the Excel file Temperatur
72. the start and the command interpreter is in the main menu The cycler replies with a message of the protocol version 46 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena 6 5 4 Re Initialisation of the Cycler Software The command AN 2 ASCII 5E without any other sign re starts the software and the cyclers itself see chapter 6 5 1 The modus remote control is deactivated 6 5 5 Auto Restart A cycler with an active block which is switched off or terminates as a result of a power failure checks the aborted temperature program and if it is without an error the program starts again from the point of the deactivation of the block The communication is the same as above The auto restart status can be controlled with the command BSTT in the menu BLCK 6 6 Setting ofthe Communication The following attributes of the communication are deactivated after the initialisation and have to be switch on with the corresponding commands 6 6 1 Check Process CRC16 The check process CRC16 according to ISO 3309 is for transmission reliability The check value will be sent in 4 ASCll signs at the end of a transmission block in front of lt CR gt without a separator The check process is applied during the asynchronous as well as during the synchronous data transfer by activating the CHCK command in menu PROT 6 6 2 Handshake Hardware handshake makes sure that no data are sent as long as the receiving device is busy A
73. tion Manual TRobot 10 2009 biometra a part of Analytik Jena 6 9 2 DESC DUO OF elle E 49 6 9 3 Main IV FST E 49 6 9 4 Menu Block IG ee een EAN rennen 50 6 9 5 Venu Block Setup BECKY BSEM iccssisccossssuinwtacunitnsnntiesageatteiiessinniendeubidectiencns 51 6 9 6 MENO NPO ee te ee ee ee 51 6 9 7 MENU GD EIER ee een 51 6 9 8 Menu Edit UIDRTEDITI 52 6 9 9 Menu Protocol PROT E 52 6 10 FORMALS of Ee EE 53 6 10 1 EXMO EEN 56 O SEIOFCOGE ee 56 6 11 1 Error Group 0xX Systemi EMOS sssrinin oine EERENS 56 6112 Emor rou p XX Edit EMO S Eee ee 57 6 11 3 Error Group 2xx Block Errors ee a en ee an nn een 57 6 11 4 Error Group 3xx Block Using Errors cccccccceecceececeeeeeeeeceeeeeeeseeeseeeeseeeaeeeaes 58 6 11 5 Error Group 4xx Memory Error 58 6 11 6 Error Group 5xx Input Syntax Emor nennen 58 OF ErorGroup Oxx Buer ENOI een ersten een engen 58 7 Trouble shooting sssini ea een a tendieren 60 7 1 Slow heating and cooling cece ceccceeccceeeceeeeceeeceeeeeeeceeeceuceeeeeceeeeseeesneeseeeseeeesneeseeees 60 1 2 AUGS e ee 60 7 3 Autorestart due to unrecognised power TIoalure 60 7 4 Opening and closing heated lid ottlImne 60 TD Recommendations for the use of 384 well Tbhemmocvcers 60 8 Maintenance and repair uuusuanunnanunnnnnonnanunnnnunnnnnnnnnnunnanunnnnunnnnnennnnennanunnunnnnnnnennanennane 62 8 1 Cleaning and Mamtenance 62 8 2 SO SA NG LE Al ceca a a ar ae ne ee Eee un ne 62 8 3 PIRI WANS ien CN 62 8 4 Replacemen
74. umtemperatur equilibrieren lassen 1 bis 6 Stunden There must be sufficient distance between the ventilation slots on the front and rear side ofthe Thermocycler and a wall or another instrument min 10 cm Danger of overheating Das Ger t sollte mit einem Mindestabstand von 10 cm zwischen der Ger ter ckseite und einer Wand oder anderen Gegenst nden aufgestellt werden Gefahr der berhitzung This instrument is designed and certified to meet EN 61010 1 safety standards It should not be modified or altered in any way Alteration of this instrument will void the warranty void the EN61010 1 certification and create a potential safety hazard Das Ger t ist gebaut und zertifiziert nach dem Sicherheitsstandard EN 61010 1 Es darf in keiner Weise modifiziert werden Jegliche Ver nderung des Ger tes f hrt zum Verlust der Garantie der EN 61010 1 Zertifizierung und ist eine potentielle Gefahrenquelle Place the TRobot Thermocycler on a stable non flammable surface ina dry safe environment Stellen Sie den TRobot Thermocycler auf eine stabile nicht entflammbare Unterlage in einer sicheren und trockenen Umgebung biometra a part of Analytik Jena The TRobot is designed for operation in robotic workstations Access during operation should be restricted The instrument is equipped with a motorized lid that opens and closes automatically Never put your hands inside the instrument during the motorized lid is in action The closing
75. utes runtime DDDDD HHHHu complete runtime 0 59940 minutes scbitemp DDD DD HHHHs temperature at block error 7 00 101 70 scpeltcur EDD D I HHHHs peltier current at block error 0 99 99 scpuls HHHH HHHHu pulse width at block error U FEEF slope D DD HHHHu set slope 0 01 1 99 2 0 5 0 spreadfac DDDDD HHHH spreadfactor for gradients 0 FFFF ssernr CCCCCCC ss CCCCCCC serial number 0000000 9999999 stepnr DD HHHHu step number 1 99 sversion DD DDCC DD DDCC software version 00 01tr 99 99tr syncdata see short see short format synchronous data are used for see range of single format of single of single the control of the activity of a parameters parameters parameters block Contents blocknr bistatus acttime htimea stepnr cyclcount 0 lidtempa cooltempa tempform bltempa see chapter 6 3 1 Instruction Manual TRobot 10 2009 55 biometra a part of Analytik Jena systatus BBBBBBBB HHHHu system status 0 FF bit O low voltage bit 1 block error controller d cooler bit 2 bit 3 bit 4 remote control error bit 5 memory error bit 6 software error tempform C HHHHu temperature format of A tempinc DD DD HHHHs set temp increment C 0 20 00 timeinc DDD HHHHu set time increment s 0 240 timeoutlen DDD HHHHu length of timeout 0 255 64ms waittime DDD HHHHu waiting time of repeat function 0 254 64ms if crc16 check is selected
76. ware main screen see chapter 5 3 5 4 9 Show remaining time Press button Memaining time In the Thermocycler Manager Software main screen see chapter 5 3 to let the software display the remaining time of started programs A new window opens that shows the remaining time Thermocycler Manager u Remaining time 2 hourls 15 minutets 5 5 Memory management The Thermocycler Manager Software provides various functions for the management of programs and directories on the computer or on Thermocyclers The button Memory management will take you to an extra window for the memory management Memory management ct Program I in Directory m es program Name AAA Copy program Delete program Delete directory Save all programs ta PL Download programs from PL Make a service file Direct command 5 5 1 Find program The Thermocycler Manager Software offers several options to search for a program After pressing the button Find program see chapter 5 3 a new window opens where the parameters for the search can be set Instruction Manual TRobot 10 2009 31 biometra a part of Analytik Jena Search options f Find the next program Cancel Find the active program Find the program with name Begin to search in directory number jo a Find next program Searches for the program which is based on the currently viewed program deposited at the next higher memory
77. y delete 33 Disposal 68 Errorcodes 56 Firmware update 62 Heated lid 60 Heated Lid 27 Installation 15 Lid 27 Maintenance 62 Memory management 31 Memory management exit 36 Motorized lid 19 20 Network settings 23 Operation Voltage 15 Packing 65 Plasticware 63 Plate lifter 6 Progam copy 32 Program create 2 delete 33 edit 28 Instruction Manual TRobot 10 2009 biometra a part of Analytik Jena find 31 load 29 pause 30 remaining time 31 save 29 save to PC 34 send 30 start 30 stop 31 Transfer to Thermocycler 35 view 32 Programming screen 27 remaining time 31 Repair 62 Return shipment 64 Serial Communication Protocol 39 Service 62 Service Info File 35 Technical specifications 8 Temperature data 36 Thermocycler Manager exit 36 Thermocycler Manager Software 23 Installation 23 Main screen 23 TRobot front view 18 rear view 19 Trouble shooting 60 Warranty 70 71

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