User Manual
Contents
1. 18 Dual scope channel can be checked at once by pressing Dual view button when 2 scope unit is connected to station unit To close dual view mode double click it 000 00 00 If the movie should be finished while recording press Stop button Then the confirmation message as below will be shown Press OK button Unexpected problems such that the power is off suddenly or the scope unit is not connected to station unit while movie recording could be happened Then the error message as below would be shown after restarting the instrument Recorded data before getting in problem is saved in the disk of instrument temporarily So press OK button if the movie recording should be continued 8 Recorded data can be opened in Data menu page 20 JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 19 Data 1 All of saved data could be checked in Data menu Monitoring data including movie file and time lapse images were saved Open data H in each of folder set up by user And other data except monitoring are saved in a default folder Captured Images Results 1E Default folder for captured images mnjh mnvb movie_name movie name Monitoring data folders set up by user movie name movie name3 movie name4 List category 2 There are sub folders in case of dual monitoring data Each control sample and experimental sample data is in the sub folder Results Capt2. Ee Neh EU JuLI Br EN rm Model JULI BRSC Scope unit is only used in conjunction with the Station unit Product overview An increasing number of researchers are using live cell imaging to study cellular functions The JuLI Br a smart Bright cell movie analyzer was designed for a variety of biological experiments JuLI Br uses state of the art optics to get live cell images from various cell culture dishes It is able to detect the quantified cell confluence results with low variation and make growth curve using image based analysis JuLI Br is able to capture sequential time lapse bright images which can be converted to movie files avi automatically The compact design allows you to install the system in your cell culture incubator easily It can be used to compare control and experimental samples using dual systems optional concurrently Features of JuLI Br live cell movie analyzer Compatible with cell culture incubator Operate inside cell culture incubator with compact amp compatible design Time lapse image capture amp recording movie Sequential time lapse images are stored and can be converted to movie file automatically Automated quantitative cell confluence analysis Quantified cell confluence results with low variation and growth curve 10 1 color LCD touch screen Easy to use system control Semi automated focusing through LCD Focus adjustment using focus inte
3. User can review saved image from JuLI Br in the PC User can recalculate confluence of all images and edited movies To install PC software follow the directions as below Check to ensure that the PC is connected to Internet and then the PC software could be installed Installation 1 Connect the supplied USB driver to the computer Then open the file JuLI Br PC software 2 The start up dialogue of the ae ee software as shown like left image will Pet ed rectal uL ee ee appear Ir ergeet Tult vu eet ad of applicamons mone eana aei Chick but o panra oe Cancel Set Sein PE Ex 3 Click Next to start installation Zeap ill re A7 Be PC erg fe Folien Schier D dgeggs Ach Text H rai sa Ei be ne eec 8 derer Noleles rho Bree SOGITIDL TIC Ug owas Akt LM of ee d par im rere 32 Operation PC software Installation Browse For Folder Select a folder in the bet below then cick CK C WProgeam Piles ctis WuLT PC gt d Progam Files laj Program Files x86 0 fies meth TO s Et Framework fe are to cre dy wad ant anderen af fe ght nid EE ee Seaibed in te kores terme Fou must accept the kee me before you an retai he gue Welcome to Setup MICROSOFT SOFTWARE SUPPLEMENTAL fren Pe Page Down hey Fr ze mere feat A Ehren reed ord ACCEPT the terme of fe Lom Agron LOO NOT ACCEPT the tema of the Leem Agreement em gend infermeton about
4. Ut u D E Delete function can be used for folders of monitored data and files in default folder Captured Images but not for each files in monitored data folder Multi select 00 00 To delete several folders or files at once select all of them after pressing Multi select button 2 Delete the folder or file with Delete button If Captured Images folder is selected all of image files in the folder will be deleted but not the folder Only main folder can be deleted not sub folders individually in case of dual monitoring data 3 Press OK button when confirmation message shows up JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 27 4 Check confirmation message Delete complete 28 Settings 1 Setting menu works for each scope unit if 2 unit is connected to station unit Press Channel tab to select each scope unit Focusing Settings Set the recommended parameter value corresponding to cell line Sensitivity refers to the contrast of the objects from the back ground Increased sensitivity provides better recognition of fainter objects in bright field Background level decreased background level provides better recognition of fainter objects in bright field Parameter Cell line Recommendation A Default HepG2 GH3 Hep3B Sensitivity level 9 A549 MCF7 Background level 2 SH SY5Y SCN2 2 NIH 3T3 F9 HeLa DU 145 B LNCaP Sensitivity level 10
5. Background level 2 C U 2 OS Sensitivity level 10 ADSC Background level 8 JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 29 Update data amp time Control LED power Update software amp firmware Set up local date amp time with up and down button DD MM YYYY HH mm Adjust the LED power intensity tapping the bar to point Default LED power is 10 Default 1 Periodically NanoEnTek adds functionality and other improvements to JuLI Br user interface We recommend keeping your JuLI M Br update with the latest software and firmware using update button If you have any questions about software updates contact sales 9 nanoentek com 2 Visit JuLI Br website at www nanoentek com to download updated software which is supplied as a zip file in your desktop 3 Unzip the zip file and save the software in a USB driver 4 nsert the USB drive into the USB port on JuLI M Br 30 5 Check if the version of software and firmware are the latest one Press Update button The update process takes a few seconds 6 After updating Press OK button to restart JuLI Br automatically Check the disk space of JuLI Br If no disk space left saved data should be deleted in Data menu Check the disk on page 20 space JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 31 PC software PC software is designed to manage data from JuLI Br Introduction l l l
6. NanoEnTek Inc 43 Cleaning amp maintenance Clean the surface of JuLI Br instrument with a damp cloth To clean the LCD screen turn off JuLI Br instrument disconnect the power cable and clean the LCD screen with a soft cloth lightly moistened with LCD cleansing detergent Cleaning the screen with excessive force can damage the LCD the screen Wipe the screen dry immediately If liquid spills on JuLI Br turn off the power immediately and wipe dry JuLI M Br does not need regular maintenance To troubleshoot problems with JuLI Br contact Technical Support page 52 Z IMPORTANT Never disassemble or service JuLI Br by yourself Unauthorized repairs may damage JuL Br or alter its functionality which will void your warranty Contact sales amp nanoentek com or your local JuLI Br distributor to arrange for Service Always wipe surfaces with ethanol soaked paper towels Do not directly spray ethanol anywhere on Jul TH Br Avoid exposing JuLI Br to UV light UV light may degrade components including plastic Damage from UV exposure is not covered under the manufacturer s warranty 44 Troubleshooting Installation JuLI Br does not power up Check on off switch on back side of unit Check power source or contact your distributor LCD screen is black Touch the LCD screen with your finger Check if power supply is connected and power switch is on Reset the power bu
7. drive is not inserted into USB port you can not use this function 2 Select the folder or file by tapping it once Settings 00 00 3 Several folders or files can be selected at once using Multi select button If the wrong file is selected press Multi select button again Multi select 4 Press the Save to USB button Only main folder can be saved not sub folders Save to USB individually in case of dual monitoring data 24 5 Confirmation message Save complete is shown saving process is completed Experimental sample data channel 2 is included in control sample data channel 1 folder in case of dual monitoring Channel 1 JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 25 Rename 1 If you want to change folder or file name tap the file or folder Rename 00 00 Rename function can be used for folders of monitored data and files in default folder Captured Images Not for each files in monitored data folder 2 Then press Rename button Only main folder can be renamed not sub folders individually in case of dual monitoring data 3 Enter new name of folder or file using the keypad display Then press Save button 4 Check the confirmation message Rename complete and renamed folder or file in List and Result category 26 1 Select folder or file which should be deleted from the instrument Delete u Pry o
8. saving movie file Check the unused volume of the hard disk Can not continue recording Do you want to save the recording files Recording files are going to be saved as the last state before recording stopped Data Can not play the movie properly Create movie file with time lapse images using JuLI Br PC software program Setting vull Br Update folder does not exist in the USB drive Download JuLl Br Update folder on NanoEnTek website www nanoentek com The update files do not exist in the USB drive Check the files as below image Then download JuLl Br Update folder on NanoEnTek website www nanoentek com py GER z be JullILexe Profilexml TS500SDK dll 46 PC software Movie doesn t play Load the full folder to PC software program not just avi file Can t create the dual movie using 2 different single monitoring data Check the interval and total time and match them with same conditions Graph is not be shown on the movie tap Confluence is not measured on Movie only mode However movie can be edited using PC software program ETC Fail to capture Connect scope unit with station unit using connection cable again Movie doesn t play on windows media player Install the codec provided by JuLI Br USB drive or NanoEn Tek website Can t transmit the screen to exter
9. the Open button Open data Folders and files can be opened by dragging and dropping on List region directly 2 Browse the folder or files and select it 3 If data opened successfully data list could be checked out as below image HIH 37T3 APOPTOSIS List 1120 NIH 3T3_APOPTOSIS 36 Operation 4 Click the name of image in data list Preview window will show the image and movie PC software Open data Home Conflunce Mene Dual view wy NC ke ai NS 3 Le SoHE Ka i EN GAO Rs i NO OYE RA De 3 SN DD 7 2 27 db ON Iz e Ka N ge Ge CRN EN DES SE D YA SE reate H v f N d sa NN y g eS Mer Narre SE Ak NM NIH 3T3 APOPTOSIS ED ex e A C H 4 lt 120 NIH 3T3 APOPTOSIS D In case of dual monitoring data control sample folder and experimental sample folder can be checked easily using Channel tab Channel 1 5 The image can be magnified by tapping zoom bar JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 37 1 Click the confluence menu button Conflunce 2 Select one image that needs recalculated confluence value from data list Confluence 3 Adjust the image by changing parameters recommended sensitivity level and background level corresponding to cell line Sensitivity Background level Parameter Cell line Recommendation A Default HepG2 GH3 Hep3B Sensitivity level 9 A549 MCF7 SH SY5Y Background l
10. wy ctup cpenenors to Moose Corpor eter Dem regarding the dete colecton m Dread Fe Sur Dzsczkrei Tane bimete ih em id 11mm 512 ee 4 Ifyou want to change installation folder click Browse and choose the location that you want 5 After choosing installation folder click Install to proceed with the installation 6 The computer activates the Installation of the software 7 Ifthe installation is successful the PC software program icon will appear on your desktop JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 33 Function guide When PC software program starts the interface as below image will be shown Preview window Open Save as Create Export Home Confluence Movie Dual view dual movie EL Conflunce o Dual view Zoom bar Image information Preview window Graph Data list 34 1 Press Insert scale bar button to insert scale bar on viewing image if it is necessary Insert scale bar Click Save with scale bar button to save image with scale bar information Home Save with scale bar Harre di te ok NIH 3T3_APOPTOSIS a GI Hal ie E H d 2 a T rte fh KE ELE ER ie KK Sep JT est SEMEN 1120 NIH 3T3 APOPTOSIS C s A E yp DU CAS j 2 e i P dP af wi SESSUALI Lo P mmm SI 4H Cb De Rap FE a Tim i umm Mel JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 35 1 Click
11. JuLT Br H Live cell movie analyzer User Manual Nano nlek Table of contents Product components 2 Product overview 3 Installation 4 5 Description 6 10 Operation 11 43 Focusing 11 15 Adjust the focus 11 12 Confluence 13 14 Capture 15 Monitoring 16 19 Data 20 28 Open data 20 22 Screenshot 23 Save to USB 24 25 Rename 26 Delete 27 28 Settings 29 31 Set up parameter 29 Update date amp time 30 Control LED power 30 Update software amp firmware 30 Check the disk space 31 PC software 32 43 Introduction 32 Installation 32 33 Function guide 34 Home 35 Open data 36 37 Confluence 38 Edit movie file 39 Dual view 40 41 Create dual movie 42 Export data 43 Cleaning amp maintenance 44 Troubleshooting 45 47 Warranty 48 Safety precaution 49 50 Product specifications 51 Technical support 52 JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 1 Product components JuLI Br is shipped with the following components Please check all components listed below when an instrument was delivered If any items are missing or damaged contact your local distributor or e mail sales 9 nanoentek com B 00 M Scope unit Station unit Power cord Connection cable JULI BRSC JULI BRST 4 pcs set for U S Canada 3M 1EA 1 EA Taiwan Japan Europe or UK 1 EA Went MF OM BL M USB drive User manual Quick manual 4 GB 1 EA 1EA 1EA e User Manual Quick Manual Lei RA
12. eration Focusing Confluence 6 Check confirmation message Capture complete Saved data can be opened in Data menu on page 20 The images below show the guideline for focus to obtain accurate cell confluence Du 145 Parameter A Good setting Exposure 4 Brightness 1 Normal setting Exposure 6 Brightness 3 A e Confluence 36 22 Bad setting Exposure 6 Brightness 5 Confluence 43 03 14 1 Adjust the focus of JuLI Br using Focusing menu Capture on page 11 12 2 Press the Capture button to acquire the image 3 Type the name of image that should be saved Then press Save button Time wen 4 Check confirmation message Capture complete Press OK button 5 Saved data can be opened in Data menu on page 20 JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 15 Monitoring 1 Each scope channel can be viewed by pressing Channel tab if 2 scope unit is connected to station unit 000 00 22 2 Adjust the focus of JuLI Br and select the region that should be monitored using Focusing menu on page 11 12 Press Setting button 000 00 22 3 Press the Name form to type file name for saving images or movies Then press Save button 16 4 Choose the monitoring mode among Growth rate Wound healing and Movie only Growth rate fe Mode Wound healing Movie only Movie only means
13. evel 2 SCN2 2 NIH 3T3 F9 HeLa Du 145 B LNCaP Sensitivity level 10 Background level 2 C U 2 OS ADSC Sensitivity level 10 4 Click the Confluence button the value of confluence would be recalculated by new sensitivity level and background level Confluence 5 If the confluence value from step 4 is good enough to use then check Apply box for recalculating confluence value of all images in same folder by new sensitivity and background level _ Apply 6 Confluence graph would be redrawn before after 7 Click Save as button if recalculated data should be saved 38 1 Recorded movie can be shortened easily through PC Edit movie file software Set the start point and end point by moving the bar Dual movie creation Fapor 1221 APOPTOSIS HeLA 40hr 1221 APOPTOSIS HeLA 40 0000000 E 000 10 00 000 20 00 000 30 00 000 40 00 000 50 00 001 00 00 001 10 00 001 20 00 001 30 00 Time Hour Min 001 40 00 001 50 00 Confluence 96 3 Click Save as button if edited movie should be saved save as 4 Select the folder to save edited movie and press OK button towne for Fide Make New Tode ox j Cem JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 39 1 Dual monitoring data can be checked at once using Dual view menu button Click Dual view menu button Dual view 3 Individual movie files from control sample and experimental sam
14. hey cannot be reproduced translated published or distributed without the permission of the copyright owner JuLI Br User Manual Website www nanoentek com E mail sales 9 nanoentek com il Manufactured by NanoEnTek Inc 12F 5 Digital ro 26 gil Guro gu Seoul 152 740 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 The information in this manual is described as accurately as possible The changes involved in firmware and software may be applied without notification Copyright 2012 by NanoEnTek Inc All rights reserved Published in Korea Documentation NESMU JUB 001E Revision History V O O Date Oct 2012 V 1 0 Date Jan 2013 V 1 5 Date May 2013 V 1 6 Date Jan 2014 JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 53 NESMU JUB 001E V 1 6 NanotnTek NanoEnTek Inc HQ 12F 5 Digital ro 26 gil Guro gu Seoul 152 740 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 A TET A mne 5627 Stoneridge Drive Suite 304 Pleasanton CA 94588 USA Tel 1 925 225 0108 1 888 988 0108 Toll free Fax 1 925 225 0109 Website www nanoentek com
15. ive cell movie analyzer 2012 NanoEnTek Inc 11 Operation f While viewing cells use the Focus knob or Focus interface Focusing to further adjust the focus Adjust the focus coarse focus interface fine focus interface Type of focus interface would be switched by tapping circle point Coarse focus is larger knob which moves up or down rapidly to get the sample into coarse focus Fine focus is smaller knob which moves short distances slowly and is used to get the sample into sharp focus The images below show the reference images to adjust focus Du 145 Parameter A Exposure 4 d setti Good setting Brightness 1 Exposure 6 Normal setting Brightness 3 Exposure 6 Bad settin g Brightness 5 Focusina 1 Adjust the focus of JuLI Br using Focusing menu Confluence on page 11 12 2 Press Confluence button Confluence 3 Value of confluence would be shown If the value of confluence should be more accurate try again after adjusting parameter in Setting menu on page 29 Parameter is the recommended value for corresponding to cell line If confluence should be recalculated press Confluence button after pressing Preview button first 4 Press the Capture button to acquire image with confluence 5 Type the name of image that should be saved Then press Save button Save JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 13 Op
16. just monitoring cells without a confluence 5 Press the Total time to set up the total running time Press the Interval to set the time interval Select on for AVI file to create AVI file with time lapse images Totaltime GGGIOG 00 ats 000 00 00 Movie setting Images 0 AVI file on scope CH ON CH2 Ner Select scope channel should be monitored Choose both channel 1 and channel 2 in case of dual monitoring AN IMPORTANT Warm up scope unit for 1 hour in incubator before making a movie If not it might be the cause of uncertain data or unclear movies Z IMPORTANT Cell culture flask should be wetted by the culture media before making movie JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 17 Time interval should be set up more than 1 minute for the best quality result in case of growth rate and wound healing but in case of dual monitoring more than 5 minutes is recommended The number of total images will be displayed automatically when data value of Total Time and Interval are set up E A GE D Apply 6 Press the Apply button to save all options If the disk volume of the instrument is not enough to save recording data then the error message as below will be shown Please try again after deleting data in Data menu on page 20 7 Press the Rec button to begin recording JuLI Br displays live cell images continuously during recording
17. ly Operating 5 40 C temperature Maximum relative 20 95 humidity Instrument specifications Cat no JULI BR04 Single set 1 Scope amp 1 Station JULI BRDO4A Dual set 2 Scopes amp 1 Station JULI BRSC 2 Scope JULI BRCM Counting starter kit JULI BRTB XY Stage Magnification Objective 4X and digital zoom 400X Image resolution 2560 x 1920 pixels BM Exported formats JPEG image AVI movie CSV raw data Display 10 1 LCD touch screen Light source White LED Dimension amp weight Scope unit 300 x 190 x 188 mm 4kg Station unit 282 x 285 x 160 mm 3 2kg Storage 320 GB Hard drive 4 GB USB drive JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 51 Technical support Visit the our Website at www nanoentek com for Technical resources including manuals FAQs etc R Technical support contact information Additional product information and special offers For more information or technical assistance please call or email NanoEnTek Inc HQ 12F 5 Digital ro 26 gil Guro gu Seoul 152 740 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 NanoEnTek USA Inc 5627 Stoneridge Drive Suite 304 Pleasanton CA 94588 USA Tel 1 925 225 0108 1 888 988 0108 Toll free Fax 1 925 225 0109 Email sales 9 nanoentek com Website www nanoentek com 52 All the materials in this user manual are protected by Korean and international copyright laws T
18. mages to PC Display port connect to external monitor resolution 1366 X 768 pixels make sure to turn off an instrument before connecting Scope unit gt Connection port port to connect scope unit with station unit lt Scope unit gt Connection port lt Station unit gt Display port USB port Power button JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 7 Description Rear view lt Station unit gt Power inlet connects JuLI Br to an electrical outlet using the supplied power cord and the appropriate plug based on the electrical outlet configuration in your country On off switch the main power switch It is not necessary to use the on off switch for day to day operation of the instrument Connection port port to connect scope unit with station unit Scope unit gt Fan machinery cooling system lt Scope unit gt Fan Station unit Connection port On off switch Power inlet User The user interface of JuLI Br Live cell movie analyzer provides new tools to expand cellular research through proprietary software Insightful data can be acquired such as proliferation assays interface Focusing menu Focusing menu Scope unit channel Zoom in Navigation Zoom out Sacle bar Focus interface Exposure Brightness Capture Confluence Monitoring menu Monitoring menu Focusing Settings Gra
19. n an industrial environment In the documentation for the user a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 49 Safety precautions Review and follow the safety instructions below Symbol Meaning A Caution amp Warning D Protective earth Ground A This instrument and consumables conforms to the Declaration of Conformity FCC Compliance This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at his own expense 50 Product specifications Environmental conditions Electrical input AC 100 240Va c 1 9A 100W Frequency 50 60 Hz Installation site Indoor use on
20. nal monitor through display port Check the resolution of the outer monitor resolution 1366 X 768 pixels Make sure to turn off instrument before connecting JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 47 Warranty NanoEnTek provides 1 year warranty service for defects of material and workmanship If any defect occurs in JuLI NanoEnTek provides free repair service for the defective parts at its discretion The following defects however are specifically excluded Defects caused by improper operation Repair or modification done by anyone other than NanoEnTek or an authorized agent Damage caused by substituting with alternative parts Use of fittings or spare parts supplied by anyone other than NanoEnTek Damage caused by accident or misuse Damage caused by disaster em bah Corrosion caused by improper solvent or sample For your protection JuLI Br units being returned must be insured against possible damage or loss NanoEnTek cannot be responsible for damage incurred during shipment of a defective instrument It is recommend that you save the original packing material in which the instrument was shipped This warranty is limited to the replacement of defective products For any inquiry or request for repair service contact sales 9 nanoentek com or your local distributor 48 Safety precautions Review and follow the safety instructions below If water or other material enter
21. per ventilation and prevention of overheating of electronic components 2 Plug the supplied power cord into JuLI Br 3 Connect scope unit to station unit using supplied connection cable For dual monitoring 2 9 scope unit also should be connected to station unit Cm i S z y i H 2 SS 3 3 N IMPORTANT Connect to the 2 scope unit after connecting to the 1s scope unit completely 4 Plug the power cord into the electrical outlet Be sure to only use the power cord supplied with the instrument Powering the instrument with an unapproved power cord may damage the instrument 5 When JuLI M Br is ready to use start JuLI Br by pressing the Power button on the station unit JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc Description Front view lt Station unit gt LCD touch screen located at the front of the instrument it contains buttons for necessary functions and displays bright field images lt Scope unit gt Focus knob is used to adjust the image quality to obtain better bright cell images Stage place sample here White LED provides bright image viewer White LED Neck Stage Focus knob lt Scope unit gt Station unit LCD touch screen Description Side view lt Station unit gt Power button turn an instrument on and off by pressing the Power button USB Port insert a USB drive to save and transport i
22. ph Confluence File Name Scope unit channel 000 00 22 Monitoring data Monitoring Monitoring REC button Stop button infomation movie setting JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 9 Description User inte rface Monitoring movie Data menu Graph Data list Data menu 2 NIH 3T3 APOP File Name 70 33 Monitoring data information Zoom bar Multi Delete Save to Rename select button USB button button button User Sensitivity Data amp Time Settings Scope inte rface amp Background level information menu unit channel Settings menu Monitoring Gel 24 f en 12075 MELLE 16 9 30 Disk space Software Firmware OS LED version version version power 10 Operation e TM Focusing 1 Press the Power button to start JuLI Br Adjust the focus The main screen will be displayed 2 Place the sample on the stage 3 Adjust the focus for each scope unit if 2 unit is connected to station unit Press Channel tab to select each scope unit 4 To adjust Illumination intensity use the Exposure and Brightness bar on the touch screen Touch the arrow button a gt for more a gt minute adjustment 5 If desired use Zoom in button to magnify the desired region The scale of enlargement can be checked by pressing Scale bar tab 6 If focus of viewing region should be moved tap a point on navigation window or drag preview window JuLI Br L
23. ple can be made into one movie using Export dual movie button Export dual movie 40 Operation PC software 4 Type file name for merged movie and click Save button Dual view 5 Merged movie file will be created after merging process is done JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 41 1 Individual movie files from 2 different single monitoring data can be made into one movie using Create dual movie button Create dual movie Create dual movie 2 Select 2 different single monitoring data folder for making movie Selected movie files should have been monitored under the same interval and total time Channel 1 Channel 2 2 single movie files had been monitored under the same interval and total time 3 Click Make New Folder button and type folder name for dual movie Make New Folder BE Desktop wa Ubranes ech Homegroup A ahnssang M Computer th Network BD Control Panel amp Recycle Bin AF TI juli br p image juli br p lt wnaged Jul Br ver Dual 1 1 0 backup Make New Folder 42 1 Click Export button Information of each image in folder can Export data be exported 2 Select the folder where exported file should be saved Type the file name to save exported file Wows e Mane Ces rww 07 C tm M SS A fm mme L cONNNANNF gt e T d 1 JuLI Br Live cell movie analyzer 2012
24. rface on LCD with knob Dual system Optional Compare the control A experimental data at the same time JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 3 Installation Installing Live cell images from various cell culture dishes are directly captured in a cell culture incubator This compact design allows JuLI Br ME an instrument to be installed in an incubator and prevents contamination by maintaining a sterile environment Also the easily viewed display allows quick and convenient use in a cell culture incubator while capturing time lapse images Dimensions of Scope unit Size Width 300 mm Depth 190 mm Height 188mm Weight 4 kg Scope unit LK ei En el Station unit Cell culture incubator Z IMPORTANT JuLI Br is optimized into standard size of cell culture incubator It is recommend that the temperature should be stabilized in case of small size incubator before using the instrument Avoid exposing JuLI Br to UV light UV light may degrade components including plastic Damage from UV exposure is not covered under the manufacturer s warranty Always wipe surfaces with ethanol soaked paper towels not directly spray ethanol anywhere on Ju IN Br Installation Installing JuLI Br 1 Place the instrument on a flat dry and level surface after unpacking the instrument Allow at least 10 cm 4 inches of free space at the back of JuLI Br for pro
25. s the instrument adaptor or power inlet disconnect the power cord and contact a service person For operating environment refer to Product Specifications page 51 Do not touch the main plug or power cord with wet hands Always ensure that the power supply input voltage matches the voltage available at your location This instrument is air cooled and its surfaces may become hot during operation When installing leave a space of more than 10 cm 4 inches around the instrument and do not place any objects between the instrument and the walls Do not install an instrument on a slant or a place prone to vibrations which induces the risk of malfunction or damage of the instrument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the wall outlet and AC adapter To avoid potential shock hazard make sure that the power cord is properly grounded Be sure to position the equipment such that it is easy to disconnect Turn off the instrument before unplugging the power cord and or moving the instrument If the instrument is dropped or broken disconnect the power cord and contact a service person The warranty will be void in case of disassembly Use only authorized accessories adaptor power cord and USB drive Class A equipment is intended for use i
26. tton Focusing and monitoring menu are not activated Check the connection between scope and station Get an error message during the connecting the 2 scope unit Connect connection cable to the 2 scope unit after connecting to the 1 scope unit completely Focus Focus knob or focus interface doesn t work Check whether the focus interface is inactive or not Move the focus knob to the opposite side Monitoring Poor image Too dark and too bright Re optimize the brightness and exposure value Reset the power button Dirty image Swipe the stage with cotton swab carefully Eliminate any dust on culture dish Check for and remove any condensation on the lid of the culture dish Time lapse images become dark and bright Warm up the instrument during 1 hour before monitoring power should be on when warming up the instrument Cell culture flask should be wetted by the culture media before making movie Eliminate dust on a culture dish Check for and remove any condensation on the lid of the culture dish JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 45 Monitoring To prevent any problem such as shaking of culture dish or inflowing light pay special attention when you open or close incubator door during monitoring Can t set the interval Please check the minimum interval on page 18 Problem for
27. ured Images SINGLE i eee e Main folder ch NIH 3T3_APO chz NIH 3T3 APO HJKL 123PPY chi 123PPY j Sub folder 20 If you double tap main folder of dual monitoring data summary data view will show up 3 Data can be opened or shown by double tapping folder name in List category or tapping folder name once and pressing Result category Focusing Monitoring ta Settings 001 20 00 001 30 00 4 Recorded movie or image file can be shown by tapping its file name Movie file can be checked by pressing Play button 00 00 96 JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 24 5 Magnify movie screen or image screen widely for convenience File Rugg 22 L tze f n m 1 Insert USB drive into USB port Screenshot If a USB drive is not inserted into USB port screenshot function can not be used 2 Press Screenshot button 3 Select the desired capture region using moving icon ES D 4 Select the screen size of snapshot using size icon Snapshot capture process can be canceled by pressing cancel icon 5 After then double tap the selected region 00 00 i5 6 Type the name for saving it then press Save button 7 Check confirmation message Screenshot is saved successfully JuLI Br Live cell movie analyzer 2012 NanoEnTek Inc 23 Save to USB 1 Insert USB drive to USB port If a USB
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