ReproSet™ - GE Healthcare Life Sciences
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1. mode selector E and the time set dial F The out indicator C will give a steady light when the lamps are on and the power indicator D will flicker when the time count function is active Fig 2 Settings and indicators on the ReproSet timer Do not alter the factory set setting of E Note The factory settings of A B and E are 0 12 min and E respectively Do not alter the mode selector E from this setting Alter the time setting by turning the setting dial F to the selected time from 1 to 12 minutes ReproSet 4 User M anual 18 1125 68 Edition AA Installation 4 4 Installation ReproSet User M anual 18 1125 68 Edition AA Check the contents against the enclosed packing list and inspect all parts for damage that may have occurred during transport Report deviations or damage to your local Amersham Biosciences representative and the transport company concerned Unpack and install the lamps according to the separately enclosed instructions see also Section 6 M aintenance Place ReproSet on a bench of suitable height within reach of a grounded mains outlet Check that the bench top is level This will allow even distribution of gel solution when filling the cassette Position ReproSet so that the fan outlet is not blocked or restricted when the lamp housing lid is in a horizontal position Before connecting the unit to mains ensure that the fuse holder mains voltage selector F2. ReproSet User Manual 18 1125 68 Amersham Edition AA r Biosciences Important user information A M eaning Consult the instruction manual to avoid personal injury or damage to the product or other equipment CAUTION The Caution sign is used to call attention to instructions or conditions that shall be followed to avoid damage to the product or other equipment Be sure not to proceed until the instructions are clearly understood and all stated conditions are met Note The N ote sign is used to indicate information important for trouble free or optimal use of the product Should you have any comments on this instruction we will be pleased to receive them at A mersham Biosciences S 751 84 Uppsala Sweden Warranty and liability Amersham Biosciences guarantees that the product delivered has been thoroughly tested to ensure that it meets ts published specifications The warranty included in the conditions of delivery is valid only if the product has been installed and used according to the instructions supplied by Amersham Biosciences Amersham Biosciences shall in no event be liable for incidental or consequential damages including without limitations lost profits loss of income loss of business opportunities loss of use and other related exposures however caused arising from the faulty and incorrect use of the product Trademarks ReproSet ALF ALFexpress and ReproGel are the ex
3. clusive trademarks of Amersham Biosciences In view of the risk of trademark degeneration it is respectfully suggested that authors wishing to use these designations refer to their trademark status at least once in each article Copyright 1997 Amersham Biosciences All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted in any form by any means without permission in written form from Amersham Biosciences Amersham Biosciences reserves the right to make changes to the design and specifications without prior notice Contents Contents 1 Introduction miii saias pusdegaantoaad alessicuesass anaio 1 2 Safety AAA 2 3 Product description cccseccssccesseceenseeensesenceessnecesnseeeneesnserseneessne 3 AA A 5 ne A 6 6 Maintenance e a cece secede coe esaerea aeo FESTA Ea Seda Eea aee Naaa Fe Eear Fea Eai 10 7 Technical specifications nnnnerennsnennnnnnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 11 8 Ordering information 2 4 2 sen ae 12 ReproSet User M anual 18 1125 68 Edition AA l Introduction 1 1 Introduction ReproSet is a laboratory instrument that emits UV light It is intended for use during the photopolymerisation of Amersham Biosciences ReproGel The unit is designed for gel cassettes from the ALF family of DNA sequencers ReproSet is equipped with 4 fluorescence lamps that have the major part more than 99 of the
4. ct function use only the UVA lamps and lamp starters sold as replacement parts by Amersham Biosciences CAUTION The UVA lamps contain mercury When replacing the lamps follow your local regulations for disposal of this material We recommend you to replace all four UVA lamps and lamp starters if the light starts to flicker or at the latest after one year s use or approximately 2000 3000 exposures Disconnect ReproSet from the mains before replacing lamps or starters Replace old lamps by raising the lamp housing lid to its upright position turn the lamp 90 and gently pull the lamp towards you from the holder Before inserting new lamps make sure that the slits in the turnable ring shaped part A and the fixed part B of the lamp holders are correctly aligned according to Figure 9 Insert the lamp with the two contact pins oriented in line with the slit in the lamp holder Turn the lamp 90 so that the print on the lamp faces the reflector in the lamp housing lid Fig 9 Align the slits in parts A and B before inserting new lamps Remove the lamp starters by turning them 90 anti clockwise and pulling straight out from the holder Insert the replacement starter and turn it clockwise until it snaps into position ReproSet User M anual 18 1125 68 Edition AA Technical specifications 7 Technical specifications Light source Mains voltage Power consumption Exposed area Timer range factory
5. er M anual 18 1127 13 Note the ROP Starting the run Available at Internet web site http www amershambiosciences com ReproSet User M anual 18 1125 68 Edition AA Operation 5 Polymerising the gel When the gel has settled pull down the lamp housing lid of ReproSet and push the start button The gel will be exposed to UV light for the time set on the timer Note Unpolymerised gel solution will be polymerised unless it is screened from the UV light and sunlight ReproSet Fig 8 Polymerising the gel Preparing the cassette for the run The gel is ready for immediate use but can be stored up to 1 hour at room temperature before starting the run If necessary clean the glass plate in the area of the laser beam before mounting the cassette in the instrument Mounting the cassette and starting the run M ount the cassette in the instrument and fill the buffer chambers with buffer Carefully remove the comb when the temperature has reached running temperature Rinse the wells with buffer add the samples and start the run For more information see ALF express User M anual 18 1127 13 Note the ROP Starting the run Available at Internet web site http www amershambiosciences com Cleaning the ReproSet table Remove spilled gel solution from the protection pad with water and or detergent ReproSet User M anual 18 1125 68 Edition AA 9 6 Maintenance 6 Maintenance A 10 For corre
6. ig 3 is equipped with the correct fuse and placed so that the appropriate mains voltage marking is positioned next to the arrow symbol see T able 1 Fig 3 Fuse holder mains voltage selector Table 1 Ensure that the fuse holder mains voltage selector is equipped with the correct fuse Mains voltage Frequency Selector setting Fuse 100 V 50 or 60 Hz 100 2 5 AT 110 115 120 V 60 Hz 120 2 5 AT 220 230 240 V 50 Hz 220 or 240 1 6 AT For correct function place the protection pad on the support plate so that the logotype shows towards you 5 Operation 5 Operation A This section describes the procedure for UV polymerisation of sequencing gels for the ALF family of automated DN A sequencers Placement of ReproSet During use place ReproSet on a horizontal bench top to allow even distribution of the gel solution over the cassette surface Position the ReproSet out of sunlight to avoid exposing the gel solution to UV light during casting For the same reason polymerise the gel in ReproSet immediately after pouring Cleaning the cassette Clean and assemble the cassette according to the instrument user manual ALFexpress Recommended O perating Procedure ROP s are available at Internet web site http www amershambiosciences com When using ReproSet for polymerisation clean both sides of the glassplates since dirt on the surface will affect the light reaching the gel solution and result in uneven polymeri
7. ir light content in the UV A region 315 400 nm This region is not considered harmful to the skin or eye in normal use and thus permits an open design with easy access to the gel cassette and convenient working conditions for the user ReproSet User M anual 18 1125 68 Edition AA 1 2 Safety information 2 Safety information ReproSet complies with Safety Standard EN 61010 1 IEC 1010 1 Even though the light from R eproSet is not considered harmful to the skin or eye at moderate exposures we still recommend the user to avoid unnecessary exposure from close range For this reason ReproSet is equipped with a switch that cuts off the light when the lamp housing lid is raised to its upright position ReproSet 2 User M anual 18 1125 68 Edition AA Product description 3 3 Product description Figure 1 shows the parts of ReproSet that are referred to in the description installation and maintenance sections of this manual Start button Lamp housing PS 4x lamp starters 4x UVA lamps Mains inlet en Fan outlet Fuse holder and mains voltage selector Fig 1 ReproSet parts referred to in this User Manual ReproSet User M anual 18 1125 68 Edition AA 3 3 Product description Functional description of the timer Figure 2 shows the different settings and indicators on the timer These are The timer range A the time unit B the out indicator C the power indicator D the
8. sation With the lamp housing lid in the upright position make sure the timer display scale is set to minutes and range to 0 12 Turn the setting dial to the appropriate time for ALFexpress see ROP Starting the run Available at Internet web site http www amershambiosciences com A AY SNE Fig 4 ReproSet with the lamp housing lid in its upright position ReproSet User M anual 18 1125 68 Edition AA Operation 5 AN ReproSet User M anual 18 1125 68 Edition AA Placing the cassette in ReproSet Place the gel cassette in ReproSet according to Figures 5 or 6 Make sure that the cassette is positioned in the centre using the position guide marks on the protection pad la Fig 6 Placing the ALF cassette standing on its clamps in ReproSet Note M ake sure the solution is at room temperature before casting the gel Preparing the gel solution The gel solution is prepared according to instructions on the package insert CAUTION Use gloves and avoid skin contact with the solutions 5 Operation Casting an ALFexpress gel Remove the top from the nozzle Pour the solution slowl
9. setting Operating temperature range Operating humidity range Safety EMC ReproSet User M anual 18 1125 68 Edition AA 4 x 40 W low pressure mercury lamps emitting UV A 100 120 V 10 50 60 Hz 230 V 10 50 Hz 450 VA 30 x 52 cm 1 12 min 15 to 40 C lt 80 relative humidity up to 31 C decreasing linearly to 50 at 40 C according to EN 61010 1 LVD 73 23 EEC EN 61010 1 class installation category II 89 336 EEC EN 50082 1 11 6 Ordering information 8 Ordering information 12 Designation Code No ReproSet complete 18 1125 64 UVA lamp 4 pack 18 1125 67 Protection pad 18 1125 66 Lamp starter 4 pack 18 1125 69 ALFexpress User manual 18 1127 13 ReproSet User manual 18 1125 68 ReproGel High Resolution 17 6001 08 ReproSet User M anual 18 1125 68 Edition AA 1086 ejesddn YL Aq uapams u paund Amersham e Biosciences
10. y and evenly back and forth along the open slit between the glass plates at the bottom of the gel cassette Keep a steady pressure on the bottle during this procedure Note Lowering the pressure on the bottle can let air in and cause bubbles in the gel This can be avoided by first turning the nozzle upwards before releasing the pressure on the bottle Tap the glass plate if the gel solution tends to stick to certain spots This tendency to stick may be due to dirty glass plates and or the glass plates not being treated with sufficient amounts of ethanol before casting and can cause bubbles in the gel Unlike chemically polymerised gels there is no need to hurry when removing air bubbles If bubbles do occur carefully remove them with a bubble chaser Remove the comb first if necessary If you use the bubble chaser apply some more gel solution to the bottom of the cassette This ensures that there is Fig 7 Casting the gel sufficient solution in the cassette Let the filled cassette stand for a minute so that the capillary movement of gel solution in the cassette ceases Uncap the large bottle with the remaining gel solution and leave it under the UV light during exposure of the gel This prevents unpolymerised hazardous gel solution being left in the bottle For more information about gel casting procedures see AL Fexpress Us
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