Hardware User`s Manual Data Logger
Contents
1. gt lt Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente gt lt Nota em Protecc o Ambiental Ap s a implementagao da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instru es ou a embalagem indicam que o produto est sujeito a estes regulame2. PARAMETER VALUE Animal Single Upper Off Lower Hole Board Number Int 1 Duration Int 3 00 Level Upper 7 Level Upper 10 Module Identif 1 Send PC Off Beeps On 33 Panlab s 13 USINGTHE ACTIMETER TO MEASURE REARINGS If it is desired to measure rearings in addition to activity the lower frame should be configured as actimeter and the upper one for rearings The counts of the upper frame will be distributed over two counters depending on whether they are short FR or long SR rearings according to the level of discrimination SETUP LEVEL The time level ratio is the same as in the case of the Hole Board Data Logger 34 Panlab 14 CONDUCTING AN EXPERIMENT To conduct an experiment first connect the control unit The following screen will appear and remain visible for 3 seconds LE 8811 V 5 56 LSI Letica Scientific Instruments Figure 30 Screen that appears when you turn on the device If there is no error message the following screen should appear Stand by SET DATA TEST START Figure 31 Main screen Data Logger 35 14 1 Panlab pub ACTIMETER REARINGS Once all the parameters have been duly configured press the F4 button START to start a new experiment On doing so the control unit will show a new screen which will remain there throughout the experiment showing Ex SM 8 FM 174 7 Int 4 0 04 SS 1 FS 50 NEXT Lower STOP
3. using the lower frame and rearings using the upper frame SEPARATE FRAMES The upper frame can be disassembled separated and used as a second actimeter but it should be noted that no information will be obtained on possible rearings Data Logger 18 Panlab s 11 CONTROLUNIT MENU After powering up the Control Unit and following the parameter check the program s main screen will appear Stand by SET DATA TEST START Figure 13 Control unit main screen The four programmable sections will appear 1 SET Configuration of the actimeter parameters 2 DATA Information on the experiments 3 TEST Checks the frames 4 START Beginning of an experiment The layout of buttons Fa F2 F3 and F4 has been conceived to match the instruction on screen that is at the same height as each one in this case F1 will be used to access the SET menu F2 to access DATA F3to access TEST and F4 to access START In the course of these instructions whenever an instruction is referred to it will be assumed that the associated key will be the one used and vice versa Data Logger 19 Panlab HA toraa 11 1 SET UP MENU This section contains all the information necessary to program the variables needed to define the Actimeter s work parameters To scroll through all the variables one by one until finding the one to define simply press to scroll forward or to go back through the menu Once the desired option is
4. TROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION e Ensure that the voltage of mains is the same as that selected in the fuse holder qo Start e Check the condition of the fuses e This occurs if the parameter Send Data is set to On and the control unit is not connected to the serial port of the PC To fix this you must turn off the control unit and connect it to the PC serial port or leave the previous parameter to Off See Section Pressing F4 START button the control unit is blocked e Check that the cable is connected between the RS 232 device and the PC serial port see Figure 11 e If there are multiple devices connected in series check MAIN REMOTE connections see Figure 12 e f there are multiple control units The equipment does not send data connected in series check that all have a to the program Sedacom different ID number see section Make sure that both the serial port as the device selected in the Sedacom program are correct e f you have several control units connected in series all should be on in order not to cut communication MAIN REMOTE e Check with the TEST see section 11 3 in which infrared beams appear the problem e Remove the transparent cage or arena Without animals on the arena or in and check with the test if the problem is the cage clear the device detects gone If so the problem
5. Figure 34 Screen with Hole Board mode Ex Number of the experiment in this example 12 Int Number of the interval in this example 1 Time Time of the experiment in mm ss SH Number of long blockings FH Number of short blockings Up Lo Shows which frames are working Upper Lower or Up Lo STOP Stops the experiment 1 Conducting a new experiment using the same conditions as the previous experiment In this case press the F4 START key again to start the new experiment The number of experiments that may be carried out depends on the memory available in the control unit If there is not enough memory available to store the new experiment the experimenter will be informed and the experiment will not start 2 Changing work conditions to conduct a new experiment To do so access the different menu options press key F1 SET and change the target parameter You may then start a new experiment under the new conditions 3 Sending the accumulated information in the control unit to the PC for analysis In this case access the SEND option of the options menu Once the connections with the reception unit PC and the Sedacom program are ready press F4 SEND It is important to note that the send data option refers to all the information present in the memory of the control unit not only the last experiment so that data pertaining to experiments already sent to the PC should be cleared from m
6. for its next use e Theuser is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT OR VOLTAGE SETTING CHANGE In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment or if the equipment voltage setting is incorrect check fuses according to the following procedure 1 Remove power cord from the power inlet Data Logger Panlab tenere 2 Open fuse holder by pulling the flange with regular screwdriver Figure 2 Open fuse holder door 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert the fuse holder again positioning it according to the voltage in the AC net 115V POSITON 230V POSITION Figure 5 Fuse holder position 6 Ifthe fuses blow again unplug the equipment and contact technical service N For electrical safety reasons never open the equipment The power waRNiNG SUPply has dangerous voltage levels Data Logger Panlab 5 TABLE OF CONTENTS 1 SYMBOLS TABLE 2 GOOD LABORATORY PRACTICE 3 UNPACKING AND EQUIPMENT INSTALATION 4 MAINTENANCE 5 TABLE OF CONTENTS 6 INTRODUCTION 7 EQUIPMENT DESCRIPTION 7 1 CONTROL UNIT KEYBO
7. found press ENT to access the option Figure 14 Setup menu screens As already explained since the parameter is placed between square brackets this screen is the one with which it can be modified The F2 F3 and F4 function keys will carry out different operations These functions will now be discussed in greater detail Data Logger 20 Panlab HA teva e F1 ESC Return to the main screen e F2 Go to the previous screen If F4 is pressed before it modifies the value The value will decrease by one unit whenever the button is pressed e Go to the next screen If is pressed before it modifies the value The value will increase by one unit whenever the button is pressed e F4 ENT Activate the parameter to be modified When the identification value is believed correct press this key to store it and go back to the previous screen which as already explained provides access to other parts of the menu 11 1 1 MODE Press ENT and use to go through the different possible values for the parameter and ENT to accept the choice and go to the next one Animal Single Upper Actimeter Lower Actimeter ESC ENT Figure 15 Mode screen A parameter value will be displayed between square brackets while being edited until itis validated e ANIMAL choose between working with one SINGLE or SEVERAL animals e UPPER choose the mode desired for the upper Frame to work in
8. is due to the activity or rearing transparent cage or arena clean them with a wet cloth and try again e Clean the red translucent plastic on the inside of the frame with a wet cloth if it s dirt it can produce false positives Data Logger 40 Panlab e This is because the memory of the control unit is full or has not enough free space in the course of the experiment Send the data to the PC if you want to save see section o and clear the memory see Section 11 2 4 When starting an experiment the device displays the error message WARNING Too many intervals for the remaining memory and does not run the experiment Data Logger 41 Panlab pedido 17 PREVENTIVE MAINTENANCE EXPERIMENT FRAMES CLEANING FRAMES SUPPORT CLENING TRANSPARENT ARENA CLEANING CHECK CABLES CONNECTION E E QIN Only when used Data Logger 42 18 SPECIFICATIONS Panlab pub POWER SUPPLY Input voltage Frequency Fuse Maximum power Conducted noise 115 230 VAC 50 60 Hz 2 fuses gmm 20mm 250mA 250V 12 W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity Storage temperature 10 C to 40 C 0 to 85 RH non condensing o C to 50 C non condensing COMUNICATIONS OUTPUT Standard interface RS232C Connector Delta 9 contacts connector ACTIVITY
9. of times the animal stands up below the threshold Frame in Rearing mode FR Fast rearing number of times the animal stands up above the threshold Frame in Rearing mode ESC Returns to the previous screen Moves to the previous saved data Moves to the next saved data 11 2 3 SEND SEND ALL Sending Data Figure 23 Send Data screen Sends data to the PC to the Sedacom software via the RS 232 communications port Data Logger 27 Panlab HA tere 11 2 4 CLR Clears all data saved in the memory of the Control Unit Pressing CLR produces the following screen ALL DATA WILL BE LOST Are you sure NO YES Figure 24 Clear Data screen Press NO to return to the DATA screen Press YES to erase the data saved in the Control Unit memory Data Logger 28 Panlab pedal 11 3 TEST Use TEST to check the operation of the photocells An asterisk indicates that the beam is being broken by an opaque body A dash indicates that the beam is reaching the receiver Pressing TEST produces a screen like this LE 8811 25256 LSI Letica Scientific Instruments Stand by SET DATA TEST START TEST LOWER TEST Figure 25 Test screen UPPER Is the selected frame in this case the Upper one Toggle UPPER between frames by pressing the MORE button Y Is the Y axis indicates a cut beam indicates an uncut beam X Is the X axis indicates a cut
10. ARD 7 2 CONTROL UNIT FRONT PANEL 7 3 CONTROL UNIT REAR PANEL 7 4 ACTIVITY FRAMES 8 EQUIPMENT CONNECTION 9 INSTALLATION 9 1 ASSEMBLING THE FRAMES 10 STARTING UP THE UNIT 10 3 FRAME POSITION 11 CONTROL UNIT MENU 11 1 SET UP MENU 11 1 1 MODE 11 1 2 SETUP TIME 11 1 3 SETUP LEVEL 11 1 4 SETUP PC 11 1 5 SETUP SOUND 11 1 6 DATE AND TIME 11 2 DATA 11 2 1 ESC 11 2 2 READ Data Logger Harvard Apparatus 11 11 12 13 14 15 17 17 18 18 19 20 21 22 23 24 25 25 26 26 26 Panlab 11 2 3 SEND 11 2 4 CLR 11 3 TEST 11 4 START 12 12 1 USING THE ACTIMETER AS A HOLE BOARD TYPES OF BOARDS AVAILABLE 12 1 1 LE8820 12 1 2 LE8850 12 2 APPLICATION EXAMPLE 13 USING THE ACTIMETER TO MEASURE REARINGS 14 CONDUCTING AN EXPERIMENT 14 1 REARINGS 14 2 HOLE BOARD 14 3 CLEAN THE FRAMES 14 4 CLEAN THE FRAMES SUPPORT 14 5 CLEAN THE TRANSPARENT ARENA 15 WORKING WITH THE SEDACOM SOFTWARE 16 TROUBLESHOOTING 17 PREVENTIVE MAINTENANCE 18 SPECIFICATIONS Data Logger Harvard Apparatus 27 28 29 30 31 32 32 32 33 34 35 36 37 38 38 38 39 40 42 43 Panlab pol 6 INTRODUCTION The LE 8825 system is used to measure motor activity in experimentation with animals It is based on a grid of infrared cells that make it possible to determine the magnitude of motor activity by anal
11. Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet Il peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique 5 Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt
12. FRAMES Dimensions Rat 45 X 45 cm Mouse 25 X 25 cm IR cells Xaxis 16 Y axis 16 Distance between IR beams Rat 2 5cm Mouse 1 5cm DIMENSIONS Width x Height x Depth 150 mm x 66 mm x 250 mm Weight 1 41 kg Data Logger 43 Panlab pub DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direcci n del fabricante Energ a 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto IR MOTOR ACTIVITY MONITOR Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Mod le LE 8825 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lec
13. Figure 32 Screen during the experiment Number of the current experiment The number increases Ex 7 EXPERIMENT with each new experiment begun by a user Up to 200 experiments may be conducted in a single session Current interval number The main unit accumulates the Int 4 INTERVAL data and when the interval is complete it stores the data resets the accumulators and starts a new interval 0 04 TIME mm ss Time mm ss left for the interval to finish SM SLOW Number of slow deambulatory movements logged in this MOVEMENTS interval FM FAST Number of fast deambulatory movements logged in this MOVEMENTS interval SLOW N f sl Bs MOVEMENTS 9 Number of fast stereotyped movements logged in this ix STEREQTYPED interval in the case s le animal MOVEMENTS 9 x Activity in the upper frame UPPER Press F1 NEXT to Upper prend see the data ofthe lower frame If you use the upper frame to measure rearings you will see the following on the display corresponding to this frame Ex 2 Int 1 0 10 SR 1 FR 50 NEXT Upper STOP Figure 33 Screen with Rearing selection SR SLOW Number of long rearings REARING FR FAST REARING Number of short rearings Data Logger 36 Panlab 14 2 HOLE BOARD Once the experiment is under way the frame used as a Hole Board will show 1 0 10 174 FH 50 Up Lo STOP
14. Hardware User s Manual Data Logger IR Actimeter References LE8825 76 0134 LE8815 76 0127 LE8816 76 0128 LE8817 76 0131 L E8838 76 0132 LE8820 76 0133 Version Vo3 11 2014 a Panlab s l u International Calls 34 934 750 697 Pan b LAS Harvar d C Energ a 112 Domestic Call 934 190 709 A Apparatus 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Spain Info panlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab 1 SYMBOLSTABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION lt UJ O Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be exposed to a hazard Protection terminal ground connection Warning about a hot surface which temperature may exceed 65 C Warning about a metal surface that can supply electrical shock when it s touched Decontamination of equipments prior to dis
15. It may work as an actimeter ACTIMETER for rearings REARING a hole board HOLE BOARD or be inactive OFF e LOWER choose the mode desired for the lower Frame to work in The same functions as for the UPPER frame are available Once the values of each parameter have been selected press ENT to conclude the configuration of the parameters and return to the previous parameter You may press ESC to exit the menu you are in and go back to the previous one at any point Data Logger 21 Panlab 11 1 2 SETUP TIME SETUP TIME Number Int 1 Duration Int 0 10 ESC ENT Figure 16 Set up Time screen An INTERVAL is the length of time during which the data will be accumulated in the relevant COUNTERS The user may access this screen to decide the duration of the data accumulation interval i e the period during which motor activity counts should be accumulated in the respective counters for each zone fast or slow movements When the accumulation interval is complete the data is transferred to the control unit memory and sent via the RS232 port if thus established by the respective parameter the counters are reset again and a new interval starts This process is repeated the number of times set in the Number of intervals parameter The minimum duration of the interval is 10 seconds and the maximum is 59 min 59 secs The operating mode is similar to the one explained previously Thus press F4 ENT t
16. beam indicates an uncut beam ESC S Returns to the main screen If a beam is failing skip it by pressing TARE The control TARE NODE unit will ignore this cell MORE This button toggles between the Upper and Lower frames Data Logger 29 Panlab s 11 4 START Pressing this button starts an experiment LE 8811 LSI Letica Scientific Instruments Mis biz 56 Stand by Ex 1 Int 0 11 Ex 1 Int 1 0 12 SM 8 Ed 174 SM 8 174 SS al FS 50 65 1 50 NEXT Lower STOP NEXT Upper STOP Figure 26 Start menu screens WARNING If the memory is full or there is not enough capacity for the current experiment the system displays the following error message and does not run the experiment by pressing the START button Too many intervals for the remaining memory Figure 27 Full memory error screen To solve the problem send the data to the PC if you want to save see section o and clear the memory see Section 11 2 4 Data Logger 30 Panlab H pub 12 USINGTHE ACTIMETER AS A HOLE BOARD The system counts the number of times that the animal s head is inserted into the holes of the board It has 16 holes of 3omm in diameter The count is broken down into two counters SH slow and FH Fast thereby distinguishing the counts with long lock times SH and short times FH The discrimination threshold SETUP LEVEL determines the boundary between them The levels range fr
17. e is needed for transferring the data to a computer please contact your local sales delegate for more information The Sedacom software reference is composed a USB Flash key containing the software Installer License for use and Sedacom User s Manual Follow next instructions e Please refer to the Sedacom User s Manual for instructions on how to install and use the software with the present device e Aserial port RS232 communication cable provided with the present device is needed for connecting the present devices to the computer in which the Sedacom software is installed Please refer to the present User s Manual chapter 8 for instructions about how to connect this cable to the device e f the computer does not have any serial port the RS232 USB adapter is needed ref CONRS232USB contact your local sales delegate for more information WARNING the RS232 communication cable provided with the device is used for any connection from the device to any associated software Sedacom etc Even when the device is used without software in first instance the cable is to be preserved and kept in a secure place in case the need of using the system with a software arises in the future In this last case if the user loses the cable a new one should be purchased to his local sales delegate ref CONRS232 The warranty duration of the cable is the same than the warranty duration of the device Data Logger 39 Panlab poli 16
18. emory to avoid sending redundant information see the section on Clear data in the chapter describing the options menu Data Logger 37 Panlab poble 14 3 CLEAN THE FRAMES WARNING In order to clean the red transparent IR frames parts never use N neither alcohol nor alcoholic derived products otherwise stripes will appear in the transparent plastic To clean the frames you can use a lightly wet cloth and then dry them with a dry cloth If they re too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth 14 4 CLEAN THE FRAMES SUPPORT To clean the frame support you can use a lightly wet cloth and then dry it with a dry cloth If it s too dirty you can wet the cloth with a soapy solution to clean it then remove foam with a wet cloth and finally dry it with a dry cloth 14 5 CLEAN THE TRANSPARENT ARENA To clean the transparent arena you can use a lightly wet cloth and then dry the transparent walls with a dry cloth If they re too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth WARNING In order to clean transparent walls never use neither alcohol nor alcoholic derived products otherwise stripes will appear in the transparent plastic Data Logger 38 Panlab s 15 WORKING WITH THE SEDACOM SOFTWARE The purchase of the Sedacom softwar
19. en designed Data Logger A Panlab s PC Control Some of these instruments are designed to be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Data Logger Panlab 4 WARNING Failure to follow the instructions this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition
20. mestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment gt lt Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de anos usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente PE Remarques concernant la protection de l environnement
21. n F3 or F2 Data Logger 25 Panlab ever 11 2 DATA Figure 21 Screens of DATA menu Pressing the DATA button on the main screen will access the screen shown in Figure 21 The second line contains the number of stored intervals The buttons have the following functions 11 2 1 ESC Press this button to return to the main screen 11 2 2 READ Press this button to access the saved data from the experiments Ex 1 Int 1 Lower SM 8 FM 174 SS 1 FS 50 ESC Figure 22 Example of DATA screen Data Logger 26 Panlab The information that appears is Ex 1 The number of the experiment in this example 1 Int i The number of the interval in this example 1 Lower The frame which can be Upper or Lower SM Slow movements number of displacements below the threshold FM Fast movements number of displacements above the threshold SS Slow stereotype number of movements without displacement below the threshold Frame in Actimeter mode FS Fast stereotype number of movements without displacement above the threshold Frame in Actimeter mode SH Slow hole number of times that the mouse places its head in the Hole Board below the threshold Frame in Hole Board mode FH Fast hole number of times that the mouse places its head in the Hole Board above the threshold Frame in Hole Board mode SR Slow rearing number
22. ntos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribuig o para a protecc o do ambiente gt lt Data Logger 45
23. o go to the next screen where the value can be modified Press Fa ESC to return to the previous screen Press F2 or in succession to modify this interval Once the desired interval is achieved press F4 ENT to store the value displayed e NUMBER OF INTERVALS Up to 200 intervals may be defined Therefore the experimenter may ascertain the duration of the experiment by multiplying the number of intervals by each of their duration The maximum work time is 200 59 59 2 199 56 40 i e around 8 5 days e DURATION INTERVALS This value stands for the duration of each interval It can be set from 10 seconds up to 59 min 59 sec in steps of 1 second by pressing the and keys Data Logger 22 Panlab s 11 1 3 SETUP LEVEL SETUP LEVEL Upper 5 Lower 5 ESC ENT Figure 17 Set up Level screen The LE 8825 activity monitor can detect between 2 and 100 breaks in each one of the photocells per minute Therefore these are the margins the experimenter will have to define as the value they deem appropriate for the threshold between fast or slow movements A distinction should be made between counts made by the actimeter depending on whether there is one or several animals e UPPER Is the threshold for the upper frame e LOWER 15 the threshold for the lower frame e FOR SEVERAL animals Two counters are used SM SLOW MOVEMENTS and FM FAST MOVEMENTS It measures the activity of seve
24. of each unit is connected to the REMOTE port of the previous control unit e REMOTE DB9 male connector When several control units are connected to computer the REMOTE connector of each control unit is connected to the MAIN connector of the next control unit and the REMOTE connector of the last control unit is left free e OUTPUTS Two BNC connectors one for each activity frame upper and lower Each time an IR beam is cut in the frame a 12 5 ms TTL pulse is given in the respective output e LOWER DB15 female connector used to connect the lower IR frame to the control unit e UPPER DB15 female connector used to connect the upper IR frame to the control unit Data Logger 12 Panlab 7 3 CONTROL UNIT REAR PANEL POWER LE 8825 Made in Spain Figure 9 Control unit rear panel e POWER Main switch power inlet and fuse holder Data Logger 13 Panlab toners 7 4 ACTIVITY FRAMES UPPER FRAME LOWER FRAME FRAME SUPPORT DB15 Figure 10 Mouse activity frames Figure 10 shows mouse activity frames but all of the same elements are also present in rat activity frames The only difference is that the rat frames are larger The frames are placed in the frame support which has two guides to align the frames that are fixed to the guides by two large black plastic screws These two guides can turn from a vertical to a horizontal position to reduce space when the frames support i
25. om 1 to 15 LEVEL TIME sec 1 0 025 2 0 050 3 0 075 4 0 125 5 0 175 6 0 25 7 0 325 8 0 5 9 0 75 10 1 11 1 25 12 1 675 13 2 5 14 3 325 15 5 The times greater than the selected threshold are considered as SH and those who are below the selected threshold are considered as FH The module LE8825 can be connected together two panels of holes or combinations of a board and a frame of activity or rearing Although it is customary to use a single board To facilitate the choice of threshold level the display shows the shutter time produced This time appears only when using a single hole board Data Logger 31 Panlab toraa 12 1 TYPES OF BOARDS AVAILABLE There are available two types of hole boards 12 1 1 LE8820 Figure 28 Assembly of the frames with the Hole board The figure shows that the lower frame is for Poke Hole detection and the upper frame Rearing or Activity detection 12 1 2 LE8850 This is a specific HOLE POKE board Figure 29 LE8850 Data Logger 12 2 APPLICATION EXAMPLE Panlab Apparatus For the specific case of using only one hole board connected to the LOWER frame single animal 3 minute interval and level of discrimination of 1 second with sound of beeps in module 1 and no option to send the PC The SET menu must be set so Data Logger
26. posal at the end of their operative life Waste Electrical and Electronic Equipment Directive WEEE 5 gt gt gt 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Data Logger Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Panlab s 3 UNPACKING AND EOUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrumen
27. ral animals at the same time regardless of their position Each movement is logged on the SM or FM counter depending on the speed at which the movement was made A discrimination threshold SETUP LEVEL ranging from 1 maximum speed to 15 minimum speed should be established beforehand LEVEL SPEED cm s 1 100 2 50 3 33 3 4 20 5 15 6 10 7 7 5 8 5 9 3 33 10 2 5 11 2 12 1 5 13 1 14 0 75 15 0 5 Data Logger 23 Panlab s e FORONE ANIMAL In this case a distinction is made between the deambulatory movements described above SLOW and FAST Movements and stereotyped movements rocking in the same position Fast and Slow Stereotipic FS SS which will be counted according to the speed of the movement and a constant pre set threshold 11 1 4 SETUP PC SETUP PC Module Identif 3 Send data E On ESC ENT Figure 18 Set up PC screen When activated ON information is relayed to the computer and displayed immediately on the monitor whenever an interval is completed e MODULE ID ENTIFICATION This menu is needed to configure the LE 8825 system when more than one control unit is active and connected to the computer In this case each control unit must have an associated identification number ID that distinguishes it from the rest The experimenter can use up to 16 numbers for this purpose Obviously the experimenter should ensure
28. rmation although the DATA LOGGER may be the same since it monitors up to two FRAMES simultaneously Data Logger 17 Panlab 10 STARTING UP THEUNIT The activity monitor may be used in two different work modes each of which has its own specific characteristics AUTONOMOUSLY In this mode the operating parameters of each detection unit will be programmed directly through the control unit The computer only receives the data stored in each control unit and displays them on the control unit display or in a computer via the Sedacom software PROGRAMMING FROM THE COMPUTER The user has the option of centralising the configuration of each control unit from the PC This work mode is particularly useful if the experiment entails collecting data on several detection units the experimenter will not have to configure each one of the control units directly This operating mode works with both programs Sedacom and ACTI TRACK both allows the simultaneous control of up to 16 activity monitors At this point it should be made clear that while detection units will work in the same way using both options autonomously and centrally programmed from the computer they do not generally generate the same results This will now be discussed in greater depth 10 1 FRAME POSITION The LE 8825 actimeter allows two work modes SUPERIMPOSED FRAMES This is the usual working mode The experimenter will be able to measure activity
29. s not in use Frames have 16 IR beams in an X axis and 16 IR beams in a Y axis The frames have a DB15 connector to connect them to the control unit connectors labelled UPPER and LOWER A transparent arena is also available It makes it possible add walls to the area inside frames to prevent animals from escaping and divide the area into four smaller parts to work with two animals at the same time Data Logger 14 CONNECTORS Panlab 8 EQUIPMENT CONNECTION The necessary connections are shown in the following schematic Figure 11 Equipment connection The necessary cables and connections are listed in the following table FROM TO CABLE LE 8825 MAIN PC Serial port DB9 Cable LE 8825 LOWER Lower Frame DB15 Cable LE 8825 UPPER Upper Frame DBas Cable P N 9 Data Logger 15 Panlab computer is able to control up 16 control units connected in series but the following rules must be respected when connecting more than one control unit to the same computer esy Veo Q 9 Lower UPPER Figure 12 Example of a 3 control units connection e Allunits must have different IDs so that the computer can identify them e It is not necessary for all ID numbers to be correlative For example it is as correct to work with cages 1 2 and 3as it is to work with cages 1 5 and 7 e Iti
30. s not necessary for cages to be physically located in the order of their ID numbers For example it will be correct to work with cages 1 2 3 1 3 2 2 1 3 2 3 1 3 1 2 Or 3 2 1 e The computer serial port is always connected to the MAIN port of the first control unit e The REMOTE port of each control unit is always connected to the MAIN port of the next control unit e The REMOTE port of the last control unit is left free Data Logger 16 Panlab pub 9 INSTALLATION 9 1 ASSEMBLING THE FRAMES The Detection Unit consists of one or two height adjustable frames that should be installed in the support provided for this purpose Height is adjusted using the fixing screws located on the sides of each frame The FRAMES are equipped with 32 transmitters and 32 receivers all of which are aligned with each other The transmitters receivers are split into groups of 16 along each one of the axes x y The frames are functionally identical and may be installed anywhere on the support regardless of which one is used as the upper or lower frame If two FRAMES are used the lower one will provide information on deambulatory movement while the upper one will supply information on rearings assuming that the second frame is installed at a height which prevents it from being triggered by the deambulatory movements Alternatively the frames may be installed separately in which case each one may accumulate deambulatory movement info
31. t and report the problem to your supplier C Ensure all transport locks are removed before use The original packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones 5 A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category Il over voltage according to the General Safety Rules WARNING The manvfacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has be
32. tely The data can even be processed as trajectory tracking using the ACTITRACK program not included should be purchased separately The HOLE BOARD is an accessory that lets the user measure the animal s curiosity shown by how often it pries into the holes It uses a frame to measure the number of times the animal puts its head into the holes Another frame may be used simultaneously to measure deambulatory movement Data Logger 10 Panlab HA teneri 7 EQUIPMENT DESCRIPTION 7 1 CONTROL UNIT KEYBOARD DISPLAY Fa F2 F3 F4 Panlab HARVARD APPARATUS Figure 7 Control unit keyboard e DISPLAY Display with 4 rows and 20 columns of characters It is used to show menus IR frame tests and data recorded during experiments Fa F2 F3 and Function buttons The function of these buttons can change depending on the menu There is always a label on the display close to the button that informs as to the current function of the respective button Data Logger 11 Panlab 7 2 CONTROL UNIT FRONT PANEL MAIN OUTPUTS RS 232 MAIN At Sm RS 232 REMOTE LOWER UPPER REMOTE LOWER UPPER Figure 8 Control unit front panel e MAIN DBg female connector When several control units are connected a computer the MAIN connector of the first control unit is connected to the computer serial port and the MAIN connector
33. that each control unit has a different ID By default all control units will have the number 1 associated WARNING If two or more control units have the same number associated the computer program will not alert users to this nor will any indication appear on the central unit display Experimenters must remember that the system will not work properly in these conditions e SEND DATA This menu option allows you to transfer all the available information into the control unit s memory using the RS 232 communication port To proceed the information reception unit a PC must be connected to the control unit and the Sedacom communication program supplied with the motor activity monitor should be running WARNING If Send Data is set to ON and RS 232 cable is not connected between computer serial port and control unit as soon as you press START to begin an experiment control unit will be blocked and you must re stat it To prevent this happens set the parameter to OFF or connect the RS 232 cable Data Logger 24 Panlab pub 11 1 5 SETUP SOUND SETUP SOUND Beeps On ESC ENT Figure 19 Set up Sound screen You enable disable the BEEP by setting this parameter to ON or OFF 11 1 6 DATE AND TIME SETUP DATE 04 11 2011 14 44 47 ESC ENT Figure 20 Set up Date screen A date and time will appear which may be modified except for the seconds by pressing F4 ENT and the
34. troniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mecanica Machinery directive Directive m canique Para su evaluaci n se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2012 Class B Safety of machinery EN ISO 12100 2010 En consecuencia este producto puede incorporar el marcado CE Consequently this product can incorporate the CE marking En cons quence ce produit peut incorporer le marquage CE En representaci n del fabricante Manufacturer s representative En repr sentation du fabricant CarmeCanalis ____ A General Manager I E Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 30 06 2014 Data Logger 44 Panlab Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with do
35. ysis of the position and frequency with which the experimental animal breaks the infrared beams Figure 6 LE 8825 system The system is comprised of the following parts RAT MOUSE IR FRAME LE 8815 LE 8816 FRAME SUPPORT LE 8817 LE 8818 DATA LOGGER LE 8825 HOLE BOARD LE 8820 ARENA LE 8814 LE 8833 ARENA FOR 2 SUBJECTS LE8821 LE 8823 With the following possible combinations 1FRAME 1 FRAME amp HOLE BOARD 1HOLE BOARD 2 HOLE BOARDS Data Logger Harvard Panlab H Apparatus The IR FRAME configures the detection unit where the experimental subjects are placed and consists of a 45x45 cm frame RAT or 25x25 cm frame MOUSE containing a total of 16x16 infrared beams at an interval of 2 5 cm RAT or 1 5 cm MOUSE located on the sides there are a total of 32 cells in each frame The FRAMES are held in place by the FRAME SUPPORT This component facilitates adjustment to the required height depending on the type of activity being recorded The CONTROL UNIT or DATA LOGGER can control two FRAMES independently It is designed to let the researcher configure parameters and functions definable in an experiment analyse the data generated by the breaks of the infrared beams accumulate results and visualize them in the display or send them to the PC via RS 232 communication for viewing and subsequent analysis by means of the Sedacom program not included should be purchased separa
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