Project D Autura 1000 - Instruction Manual v1.04
Contents
1. Carriage stop Remove plate from carriage Support arm with one hand and pull the pivot pin out with the other Slide arm out of carriage Take care not to drop the spring Turn the arm over and locate the spring on the other side Slide arm into carriage Make sure the arm stop is located behind the carriage stop and replace the pivot pin Autura User Manual Page 10 of 48 The head needs to be changed Disconnect tubing from head Slide 8 way head out of head cradle Slide 12 way head into head cradle Connect 12 way tubing to head 8 way set up aN WAE 12 way set up The software needs to be changed Go into the engineering menu Chapter 7 B and change the head size to 12 way The plate offset the dispense height the well depth and pump calibration will need to be adjusted to suit the new plate orientation Autura User Manual Page 11 of 48 4 Familiarising yourself with Autura 4 A General Your Autura is the most versatile strip vvasher currently available Using separate exchangeable upgrade componenis you can adapt your basic Autura to vvash any 96 or 384 vvell plates vvith V U or flat bottomed wells deep well plates factory configured of any possible well depth introduce soak times between wash stages and dispense up to three different wash reagents of any volume Autura is available with a single or double row wash heads Being a strip washer by definition m2. PROTOCOL 1 30 1 Press RUN SK to run a chain or a protocol UTILS RUN EDIT PROTOCOL CHAIN RUN START ROW 1 12 NEXT Autura User Manual Page 22 of 48 Press PROTOCOL SK to run the current protocol as normal or press CHAIN SK to select a chain to run If you selected chain this menu will appear Select the chain you want to run by using the up down keys Press SELECT SK To run the chain Autura starts the vacuum pump in order to build up the required vacuum in the waste bottle First time it will initiate a self priming cycle before it runs If you can still see air bubbles in the delivery tube you can prime again by pressing the PRIME AGAIN SK or press RUN SK Choose the row you want to start from by scrolling through using the up down keys Then press NEXT SK Choose the row you want to end on by scrolling through using the up down keys Then press NEXT SK PROTOCOL 1 RUNNING PROTOCOL 2 RUNNING RUN COMPLETE PRESS ANY KEY 1 REPEAT QUIT UTILS RUN EDIT Autura User Manual Page 23 of 48 Autura runs the selected wash protocol for the number of rows you have selected You can stop the process by pressing the STOP button on the control panel
3. open the carton and remove the washer and its components g Note Lift the main unit out of the box by putting your hands underneath the main body of the washer It will lift out of the box with its foam packing blocks on each end Following the unpacking make sure that you have all the following components Please contact your supplier immediately if you notice any of the components missing or damaged Note Do not attempt to assemble a unit using damaged components A Autura washer Cat No 1000 B Mains cable with European mains plug Cat No 5520 British mains plug Cat No 5521 American mains plug Cat No 5522 C Power supply unit Cat No 6004 D VVash reservoir blue cap vvith level control Cat No 1000 316 E Waste reservoir red cap with level control Cat No 1000 315 F User manual Cat No 1000 6000 Retain the packaging for any future shipping needs The remote power supply unit is a Switch Mode Power Supply and automatically adjusts to the main supply characteristics and will work with any voltage from 110V to 250V and 50 60Hz 1 B Positioning Autura requires an operation environment of between 10 C 40 C The fully assembled Autura 1000 occupies a work surface area of 40cm width and 55cm depth clear this area on your bench and position it to the left of this space Autura is fitted with levelling feet on each corner adjust them so the unit is horizontal and level The wash and waste bottles are then po
4. EMPTY PRESS ANY KEY To fill the wash bottle or to empty the waste bottle first unscrew the caps and lift the sensing floats vertically from the bottles and lay on a tissue paper to drain Empty or fill the bottles Autura User Manual Page 32 of 48 9 Maintenance routine These instructions are intended as a daily routine that will help you to keep the equipment fit for a long operational life 9 Safety first Always wear disposable gloves safety goggles and a laboratory coat before performing any maintenance 9 A At the end of a working day 10 1 Do not leave the wash head to dry out between wash runs The salts in your wash buffers will crystallise in the tubes and block them Between runs make sure that the dispense tubes of the wash head are left soaking in the prime trough 10 2 Fill the wash bottle with distilled water and flush all the delivery tubes and the head thoroughly by priming the system until the distilled water is dispensed through the wash head 10 3 Empty the waste reservoir and rinse them thoroughly with distilled water Saline solution that might crystallise on the level detection float will stop the system from working properly Remove the level detection rods from both bottles and let them dry on a tissue paper 10 4 Any dispense tubes that become blocked can be cleaned using the cleaning probe supplied as part of the accessory kit 10 5 t is advised th
5. If you selected chain it will automatically move onto the next protocol in the chain Autura confirms that the wash cycle is complete This message is to assure you that the cycle was not terminated prematurely Press any key to confirm Press REPEAT SK to repeat protocol or chain Press QUIT SK to return to main menu When you press QUIT SK Autura moves the priming trough under the wash head dispenses a pre set amount of wash solution into it and rests the head into the trough Autura User Manual Page 24 of 48 7 C Engineering menu This menu should only be used by the principle laboratory operator and is dedicated to changing various software configurations such as changing the machine from 8 to 12 way wash head use and the fine positional of the plate head geometry You can scroll through the menus by pressing the NEXT SK button Changes made to any of the menus will be automatically entered into the machines memory You can quit at any point In order to inhibit unauthorised personnel from making changes to the settings the access to this set of menus is via a hidden key PROTOCOL 1 30 1 The hidden key is positioned to the left of the keypad between the two rows of keys Press the hidden key four times to access the engineering az n T UTLS ED T RUN This menu will only appear if the head motor has been enabled factory option Press CHANGE SK to enter this procedure or N
6. the cap on the waste container and connect the clear Tygon tubing which exits the back of Autura onto the shorter spigot Insert the plug from the wash reservoir into its respective socket Insert the plug from the waste reservoir into its respective socket Connect the clear Tygon tubing which leads from the outlet top port of the peristaltic pump onto the top spigot on the wash head Connect the clear Tygon tubing from the top of your waste bottle cap onto the side spigot on the wash head going through tube support bracket Power up the unit using the toggle switch on the socket panel The back illuminated LCD will display the message Autura 1000 Welcome Wash Head Wash Head Cradle Plate Carrier Hp Inlet Port il ii mn Autura User Manual Page 5 of 48 3 B The peristaltic pump Tube Clamp Tube Clamp Se inlet Port Autura User Manual Page 6 of 48 3 C Changing the peristaltic pump tubing y 1 Turn off the unit Press top and bottom of the tube clip together and slide out Autura User Manual Page 7 of 48 3 D Wash Head assembly and alignment To give the best wash results Autura s wash head is adjusted to the correct position prior to shipping However the size variations between plate manufacturers may necessitate the user fine tuning this position for the type of plates most commonly used Adjustment to the heads front to back position is achieved via the softw
7. through to the next menu Press TEST SK the plate will move into the shaker position and shake Check whether the speed is correct If shaker speed is correct press SAVE SK to scroll through to the next menu If the shaker speed is not correct press CHANGE Sk Use the up down keys to change the speed Press TEST SK to check whether the shaker speed is correct If shaker speed is correct press SAVE SK to scroll through to the next menu If the shaker speed is not correct press CHANGE SK and repeat above steps Press CHANGE SK to enable the head motor or NEXT SK to scroll through to the next menu Use the up down keys to toggle between YES or NO Press SAVE SK to scroll through to the next menu HEAD MOTOR SPEED CH CHANGE NEXT QUIT m WDD HEAD MOTOR SPEED CH 255 TEST QUIT p Ge GD G GD HEAD MOTOR SPEED CH CHANGE SAVE QUIT p Ge GD aD GD DISABLE SENSORS NEXT CHANGE QUIT DISABLE SENSORS NO SAVE Autura User Manual Page 45 of 48 This menu will not appear if you chose NO in the previous menu i e no head motor Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the head motor wont turn Press SAVE SK to scroll through to the next menu Press CHANGE SK to disable the level sensors or NEXT SK t
8. wet after cycle Wash head does not move feely on its shaft Low vacuum Check that the protocol calls for the wells to be left dry Apply a small drop of oil to the shaft Check vacuum efficiency 8 Grinding noise when head moves up or down Y axis speed is set too low Increase speed through engineering menu Autura User Manual Page 34 of 48 11 Sending back for maintenance or repair Please do not send the equipment back for repair or maintenance until you have agreed the procedure with Mikura Ltd or your distributor You will receive a Return Authorisation Number RAN once you have agreed that the instrument must be returned Units will only be accepted for repair if accompanied with a fully completed and signed Return amp Decontamination Certificate A copy of the certificate is attached to this manual Units which are not accompanied with the certificate will not be accepted into the factory and returned at your expense 6 Note The equipment can be returned only after the follovving decontamination procedure has been completed 9 Safety first Always wear disposable gloves safety goggles and a laboratory coat before performing any decontamination routine 1 Empty the waste bottle completely 2 Hill the wash bottle with at least 200ml 10 Chlorine bleach solution and prime the system thoroughly 3 Disassemble wash head and immerse in 10 bleach solution for 2 hours 4 Flush waste and was
9. 0 List of Chains Autura User Manual Page 39 of 48 Chain Protocol Protocol Protocol Protocol oy A cool N 14 Appendix A Service Engineer Menus Autura User Manual Page 40 of 48 This appendix is for routines that are intended for the service engineer only Most menus are identical to user engineering menu There are however some additional menus that allow fine adjustment and return to factory default settings The following instructions focus on the menus that are in addition to the normal user engineering menus S R 1 30 UTLS RESET FACTORY DEFS RESET NEXT QUIT ARE YOU SURE RESET GD D GD X AXIS SPEED NEXT CHANGE X AXIS SPEED CHANGE SAVE To access the service engineer menu press the hidden key three times and than the key once RESET FACTORY DEFS command sets all the protocols and engineering settings to the factory s default settings The user will loose all the custom written protocols Press RESET SK to enter this procedure Press NEXT SK to scroll through to the next menu DO NOT PRESS RESET UNLESS YOU ARE ABSOLUTLY SURE Press QUIT SK to scroll through to the next menu X AXIS SPEED is the speed in which the plate carrier moves along the front to ba
10. 0 in steps of 25 For 384 plates you can enter any volume between 5 to 125 in steps of 5 Press STORE SK to scroll through to the next menu Use the up down keys to toggle between YES amp NO for sweep action A sweep is achieved by moving the plate a predefined distance along the X axis when the wash head is in its lowest position in the well This sweeping action will obtain very low residual volumes in the wells thus reducing any chance of cross well contamination Press STORE SK to scroll through to the next menu Use the up down keys to toggle between the options to dispense the wash reagent whilst the head is in its top position above the wells or in its bottom position inside the wells Press STORE SK to scroll through to the next menu Use the up down keys to toggle between the options to leave the plate full with wash solution at the end of a wash cycle or dry The option to leave the plate wet after a wash cycle is of particular use for protocols that require an extended soak or incubation period Press STORE SK to scroll through to the next menu Use the up down keys to toggle between the options In strip format the wash head will process the strip s to the set number of wash cycles that you determined previously before moving on to the next strip s In plate format Autura will allow the head to process a strip once move to process the next rows and return to process the previous strip again Press STORE S
11. Autura 1000 m ku ra Automatic Microplate VVasher eraya User Manual 04 06 2009 Issue 1 04 Autura User Manual Page 1 of 48 Autura 1000 Automatic Microplate Washer User Manual Contents 1 Unpacking hecne baa band uda Real Da dal a 2 TA Unpack ng ya READ E A aa a k b t 2 1 2 2 mans ee At ec ait A 3 PASSE DIY ce secede ten scatter yaaa R ted tetas 4 si eta conte tet eral eit a ea and 4 The pernstaltie POUNM set oes m BA os eco Pas ges eee as ea cee 5 3 C Changing the peristaltic pump tubing cece eee eeeeeeceeeeeeeeeeeeeeeaaeeeeeeeeeeeenesaaaeeeeeees 6 3 D Wash Head assembly and 1 2 7 3 E Changing from 8 way head to 12 Way 9 4 Familiarising yourself With 5522 11 000 900220 00 0 11 4 B Feamiliarising yourself with Autura system hardware componenis 11 4 C Familiarising yourself with Autura system software interface componenis 12 PRIUS es c b s 13 Plimini iheoyasler ee 13 ala la 14 o Q
12. DEPTH 120 TEST el QUIT af Autura User Manual Page 27 of 48 This sets the speed of the peristaltic pump Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Use the up down keys to change the speed Each key action increments the speed by a factor of 1 255 is the maximum speed Press TEST SK If the pump speed is correct press SAVE SK to scroll through to the next menu If the pump speed is not correct press CHANGE SK and repeat above steps This menu is designed to allow the adjustment of the wash tips in the prime trough Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the head will lower into the prime trough Check whether the head tips are about 0 5mm above the bottom of the prime trough If head tips are correct press SAVE SK to scroll through to the next menu If the head tips are not correct press CHANGE Sk Use the up down keys to step the head up and down Press TEST SK to check whether the head tips are correct Note The numbers refer to steps approx 22 steps per 1mm The position 0 is to the top of the washer Increment the number in order to move the head downwards Decrement the number if you wish the head to move upwards TROUGH DEPTH CHANGE SAVE QUIT DISPENSE HEIGHT CHANGE NEXT QUIT Haz Ge GD db GD DISPENSE HEIGH
13. E SK and repeat above steps WELL DEPTH CHANGE NEXT 157 157 VVELL DEPTH TEST QUIT WELL DEPTH CHANGE SAVE QUIT WELL DEPTH 255 TEST QUIT WELL DEPTH CHANGE SAVE QUIT Autura User Manual Page 29 of 48 WELL DEPTH is the height that the head aspirates wash reagent from the plate In most cases it is advisable to set this position to below the bottom of the wells Such setting will allow the head to rest on the bottom surface of the wells for best aspirate results Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the head will position itself at the bottom of the first row Check whether the head tips are touching the bottom of the wells If head tips are correct press SAVE SK to scroll through to the next menu If the head tips are not correct press CHANGE Sk Use the up down keys to step the head up and down Press TEST SK to check whether the head tips are correct Note The numbers refer to steps approx 22 steps per 1mm The position 0 is to the top of the washer Increment the number in order to move the head downwards Decrement the number if you wish the head to move upwards If head tips are correct press SAVE SK to scroll through to the next menu If the head tips are not correct press CHANGE SK and repeat above steps CALIB
14. EXT SK to scroll through to the next menu PLATE TYPE CHANGE NEXT Use the up down keys to toggle between 96 and 384 Press SAVE SK to scroll through to the next menu PLATE TYPE save HEAD SIZE Press CHANGE SK fo enter this procedure or NEXT SK to scroll through to the next menu CHANGE NEXT QUIT Use the up down keys to toggle between 8 12 8 X 2 and 12 x 2 Press SAVE SK to scroll through to the next menu PLATE OFFSET CHANGE NEXT PLATE OFFSET TEST Quit 1 PLATE OFFSET CHANGE SAVE QUIT PLATE OFFSET 353 TEST PLATE OFFSET CHANGE SAVE QUIT maz GD GD SHAKER INCUBATE POSN CHANGE NEXT QUIT SHAKER INCUBATE POSN 1401 TEST QUIT Autura User Manual Page 25 of 48 This menu is designed to allow the front to back alignment of the wash tips in the wells Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the plate will move under the head Check whether the head alignment is centralised over the first row of wells If head is aligned central over the first row of wells press SAVE SK to scroll through to the next menu If the head is not aligned press CHANGE SK Use the up down keys to step the plate under the wash head Press TEST SK
15. K to scroll through to the next menu Use the up down keys to toggle between YES amp NO The step over function will determine whether Autura will process the plate as if all rows are washed regardless of the number of rows to be washed This function is of particular importance whenever the soak time between wash cycles has to remain constant Press STORE SK to scroll through to the next menu EVENT ENABLED NO STORE QUIT EVENT TIME STORE QUT REAGENT BOTTLE STORE QUIT a CD STORE PROTOCOL 1 OVERWRITE QUIT a PROTOCOL 1 30 1 UTILS EDIT 1 l i Autura User Manual Page 19 of 48 Use the up down keys to toggle between YES amp NO The event enable function allows for a plate soak time after each wash Only one event can be assigned for any particular protocol Press STORE SK to scroll through to the next menu This menu vvill not appear if you chose N0 in the previous menu i e no event In this menu you can assign the duration of the event Using the up down keys the event duration will increment from 15 to max 900 seconds in steps of 15 seconds For example if you choose 120 this means a soak time of 2 minutes If you previously chose three washes the washer will fill the rows with wash solution and move the plate to the designated event position after each cycle for a period of 2 minute
16. L 1 30 UTILS PROTOCOL 1 30 1 ED T NUMBER WASHES STORE ler W d 42 af WELL STYLE por soe cur DE The EDIT SK will allow you to edit any one of 30 protocols You assign the position of the edited protocol when you overwrite it The following chart will run you through all the necessary steps Choose a protocol to edit by scrolling through using the up down keys Press EDIT SK Use the up down keys enter the number of wash cycles A wash cycle is defined as the number of times that the wash head will fill and aspirate the row of wells Press STORE SK to scroll through to the next menu Use the up down keys select the type of wells in you plate F flat bottom V shaped or U shaped Press STORE SK to scroll through to the next menu STORE WASH TOP OR BOTTOM TOP STORE WASH VOLUME STORE Quit SWEEP WELL il END VVET OR DRY STORE Quit WASH PLATE OR STRIP STORE PLATE QUIT a CD aL STEPOVER NO STORE Autura User Manual Page 18 of 48 Use the up dovvn keys select the desired vvash volume per well The volume is expressed in micro litres per well For 96 plates you can enter any volume between 25 to 150
17. LS EDIT Press UTILS SK in order to access the utilities menu s SELECT SK in order to access the prime PRIME SELECT QUIT Press SELECT SK to dispense a pre set volume through the tubes Press the same key as many times as is required to clear all air bubbles from the delivery tubes If the washer is fitted with multiple peristaltic pumps use the up down keys to select the desired pump to prime UTILITY OPERATIONS Press QUIT SK to return to the main menu PRIME SELECT QUIT Autura User Manual Page 14 of 48 5 B Plate aspirate This utility allows you to aspirate a plate s contents into the waste container This utility does not allow you to select the number of rows i e only aspirates a complete plate Press UTILS SK in order to access the utilities menu PROTOCOL 1 30 1 UTILS EDIT GD db GD UTILITY OPERATIONS PRIME SELECT QUIT UTILITY OPERATIONS Press SELECT SK Following the aspiration of all rows Autura will then return to the Utility ASE 5 QUIT Operations menu Select the Asp function by scrolling through using the up down keys Press QUIT SK to return to the main menu UTILITY OPERATIONS PRIME QUIT 6 Quick Start 6 A Running a wash protocol Autura User Manual Page 15 of 48 Subject to the machines specification Autura is suppl
18. RATE PUMP A CHANGE NEXT QUIT m GD db G amp D CALIBRATE PUMP A 100 TEST CALIBRATE PUMP A CHANGE SAVE QUIT CALIBRATE PUMP A TEST QuIT CALIBRATE PUMP A CHANGE SAVE QUIT GD db GD CALIBRATE PUMP B NEXT CHANGE FLUSH ENABLE CHANGE NEXT QUIT Autura User Manual Page 30 of 48 CALIBRATE PUMP A is the calibration of the peristaltic pump To run this function the wash bottle needs to be filled with distilled water and the wash and waste bottles must be connected to the Autura The head will need to be primed in the UTILS menu You will also need a clean dry plate Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Weigh the new plate and zero the balance place the plate into the plate carrier Press TEST SK Autura will dispense a quantity of water into each well if the Autura is set to run 96 well plates it should dispense 3001 per well If the Autura is set to run 384 well plates it should dispense 60ul per well Weigh plate if plate weighs 28 8g 300Ll x 96 wells or 23 0g 60Lul x 384 wells calibration is correct press SAVE SK to scroll through to the next menu If the plate weight is different press CHANGE SK Use the up down keys to change the calibration value Empty and dry plate re weigh the pl
19. T 140 TEST DISPENSE HEIGHT CHANGE SAVE QUIT DISPENSE HEIGHT 140 TEST QUIT DISPENSE HEIGHT CHANGE SAVE Autura User Manual Page 28 of 48 If head tips are correct press SAVE SK to scroll through to the next menu If the head tips are not correct press CHANGE SK and repeat above steps DISPENSE HEIGHT is the height that the head dispenses wash reagent into the plate The head must be adjusted so that there is a small positive meniscus on each well setting the head to about 1 0mm above the top of the plate should achieve this Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST Sk the head will position itself above the first row Check whether the head tips are about 1 0mm above the top of the wells If head tips are correct press SAVE SK to scroll through to the next menu If the head tips are not correct press CHANGE Sk Use the up down keys to step the head up and down Press TEST SK to check whether the head tips are correct Note The numbers refer to steps approx 22 steps per 1mm The position 0 is to the top of the washer Increment the number in order to move the head downwards Decrement the number if you wish the head to move upwards If head tips are correct press SAVE SK to scroll through to the next menu If the head tips are not correct press CHANG
20. are Head alignment within the wells is however achieved through the hardware There are three adjustments to the heads which you can carry out All hardware alignment procedures should be done with the plate you most commonly use in the plate carrier and the machine switched off 1 Auxiliary head alignment Move the plate with the plate carrier until its front edge is just below the first row of wash tips Gently lower the head by pushing it down until the tips are just below the top level of the plate Without forcing the plate against the tips check whether all the tips touch the edge of the plate To remedy any misalignment using the appropriate Allen key loosen the 2 off M3 socket head screws on the top of the head block align the head to the edge of the plate and retighten the screws M3 socket head screw for M3 socket head screws for level adjustment auxiliary head alignment 2 Left to right alignment Move the plate with the plate carrier under the head until the tips are aligned at the edge of the first row of wells Gently lower the head by pushing it down until the tips are level with the plate The head requires a realignment of its left to right axis if the tips do not align with the centres of the wells To remedy any misalignment using the appropriate Allen key loosen the two M3 socket head screws on the metal head locating bracket move the wash head left or right for alignment retighten the screws Autura User Man
21. at the pressure is removed from the peristaltic pump tubes to do this remove the tube clamp on the pumps 10 6 Dry out any liquid residues from the wash trough with tissue paper 9 B At the beginning of a working day 10 7 Check that the wash head is in perfect condition and none of the tubes are bent damaged or blocked 10 8 Check that the floats move freely on the level detection rods and reassemble the level detection system onto the waste amp wash bottles 10 9 Reassemble the peristaltic tubes into the pumps A click is heard when the tube clamp is properly inserted into position on both sides of the pump 10 10 Fill the wash reservoir and prime wash solution into the prime trough so that the tips are immersed 10 11 t is advised to prime the delivery system into an empty plate making sure that all head needles both dispense and suction are clear from obstruction if not use the supplied cleaning probe needle to clear any dispense tubes which are clogged 10 12 Flushing the complete system with warm clean water can clear any blocked aspirate tubes Note this may take several priming cycles to completely clear the probes 10 Trouble shooting Autura User Manual Page 33 of 48 The following guide is aimed to help you to address operational problems of the equipment Please contact our qualified service engineer if the fault continues to exist after you have completed these checks Problem Possible Caus
22. ate and zero the balance place the plate into the plate carrier Press TEST SK to repeat the dispense routine Weigh plate if plate weighs 28 8g 300Ll x 96 wells or 23 0g 60Lul x 384 wells calibration is correct press SAVE SK to scroll through to the next menu If the plate weight is different press CHANGE SK and repeat above steps Note This menu will only appear if a second peristaltic pump is fitted CALIBRATE PUMP B is the calibration of the second peristaltic pump Repeat above steps for pump A to calibrate this pump Press NEXT SK to scroll through to the next menu Press CHANGE SK to enable flushing or NEXT SK to scroll through to the next menu Note Flushing can only be done if a second peristaltic pump is fitted FLUSH ENABLE SAVE PROTOCOL CHAINING CHANGE NEXT QUIT PROTOCOL CHAINING save QuIT 8 Error messages Autura User Manual Page 31 of 48 Use the up down keys to toggle between YES or NO Press SAVE SK to scroll through to the next menu Press CHANGE SK to enable protocol chaining or NEXT SK to scroll through to the next menu Use the up down keys to toggle between No or Yes Press SAVE SK to scroll through to the next menu The error messages are there to inform you if a situation occurs that is detrimental to the smooth operation of the unit Take the necessary steps before attempting to continue the work WASH BOTTLE
23. ck axis Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the plate carrier will move back and forwards Check whether the speed is correct If plate speed is correct press SAVE SK to scroll through to the next menu If the plate speed is not correct press CHANGE Sk X AXIS SPEED 10 TEST X AXIS SPEED CHANGE SAVE QUIT G CD CH DD X AXIS ACCELERATION CHANGE NEXT 15 CHANGE SAVE 157 X AXIS ACCELERATION 8 TEST X AXIS ACCELERATION CHANGE SAVE QUIT Autura User Manual Page 41 of 48 Use the up down keys to change the speed Press TEST SK to check whether the plate speed is correct If plate speed is correct press SAVE SK to scroll through to the next menu If the plate speed is not correct press CHANGE SK and repeat above steps X AXIS ACCELERATION is the acceleration in which the plate carrier moves along the front to back axis Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the plate carrier will move back and forwards Check whether the acceleration is correct If plate acceleration is correct press SAVE SK to scroll through to the next menu If the plate acceleration is not correct pres
24. e Possible Remedy 1 LED on remote Power Supply is not illuminated s Blown fuse in plug Faulty Power Supply Unit Replace fuse in mains plug Replace power supply 2 Display amp Power LED do not illuminate power to the unit Replace the fuse and check power connection is well inserted into the socket Check the LED is illuminated on remote power supply 3 Waste Bottle Full Reagent Bottle Empty message although OK Level detection DIN plug not properly plugged into sockets Floats on level sensing rods not moving freely Check level detection connectors Clean level sensing rods 4 Dripping dispense tubes Pump speed too low Blocked dispense tubes Pump tubes not properly inserted in peristaltic pump Increase pump speed Unblock tubes with unclogging needle Open pump clip and reassemble tubes 5 Wash reagent spilling over Vacuum malfunction Blocked aspirate tubes Aspirate tubes too high over well s edge Check air lines and connectors between waste bottle and wash head Unblock aspirate tubes using syringe Lower head height in engineering menu 6 Waste bottle collapses Aspirate tubes are clogged Vacuum tube between wash head and waste bottle is kinked Unblock aspirate tubes using syringe Un kink tube check for damage 7 Plates remain wet after wash Software programmed to leave wells
25. e up and down axis Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the head will move back and forwards Check whether the acceleration is correct Autura User Manual Page 43 of 48 Z AXIS ACCELERATION If head acceleration is correct press SAVE SK to scroll through to the next menu If the head acceleration is not correct press CHANGE SK CHANGE SAVE QUIT Pettitt ttt AAAA AALAN s s n tesis it n istisna seiri istini aa Use the up dovvn keys to change the acceleration Press TEST SK to check whether the head acceleration is correct R n eee s SEES esses 2465 EA SERS tet b 2966 s 2655 ttt ttt ttl ttt 245 244 H 245 24 245 xz d f head acceleration is correct press SAVE SK to scroll through to the next menu f the head acceleration is not correct press CHANGE SK and repeat above steps MAX PUMP AVAILABLE Press CHANGE SK to change the number of pumps or NEXT SK to scroll through to the next menu CHANGE NEXT QUIT A EENETI ATETEA ANEETA EAEE s s ses Hisse s s isin n ise Hisse irisi ATETEA AETAT RER RER m m Use the up down keys to toggle between A or C Press SAVE SK to
26. eans that the Autura washes in a strip format and not as a full plate washer You can choose to wash any number of strips as long as they are arranged as adjacent rows l e using a double row 8 way head you cannot choose to process rows 1 2 and 5 6 whilst skipping row 3 4 The washer is designed as an open frame transparent unit in order to facilitate maintenance and upgrades as well as allowing easy integration with existing robotic systems 4 B Familiarising yourself with Autura system hardware components For added safety Autura washers are fitted with a remote 12v 4 3A power supply Therefore there is no mains voltage apparent within the unit The remote power supply plugs into your mains supply socket and should be kept away from the unit and dry at all times The Autura system operates by moving the microplate under the wash head The wash head then moves into the wells to aspirate any liquid within them and dispense the wash solution from a controlled height The wash head is located in a cradle and is magnetically connected to allow free floating up and down this is to avoid damaging the wells coating and the tips whenever the tips come into contact with an obstacle or when resting on the bottom of the wells The fluid pathways on Autura are very simple All tubes are exposed to allow you to pin point any air bubbles that may be apparent in the tubes and to easily maintain the system The wash liquid is pumped from the wash rese
27. ess SELECT SK In order to access the chaining menu CHAIN QUIT SELECT CHAIN Select the Chain to be edited by scrolling through using the up down keys 1 SELECT QUIT Press SELECT SK In order to access the chain editing menu SELECT QUIT SELECT CHAIN Select the first Protocol to be chained by scrolling through using the up down keys LINK 1 PROTOCOL 1 SAVE QUIT Autura User Manual Page 21 of 48 Press SAVE SK To move to the next link menu LINK 1 PROTOCOL 5 11 QD GD LINK 2 PROTOCOL Select the second Protocol to be chained by scrolling through using the up down keys 16 2 DD LINK 2 PROTOCOL SAVE Quit i bea W 00 db LINK2 PROTOCOL Repeat the above steps to chain more Protocols If you don t want to chain anymore Protocols use the up down keys to select Press SAVE SK To move to the next link menu When all 4 links are saved it will return to the utilities menu Press SAVE SK To move to the next link menu m SAVE QUIT UTILITY OPERATIONS PRIME SELECT QUIT When chaining is enabled the run menu will change The following chart will run you through all the necessary steps to run a chain or a protocol You can quit the running of a chain or a protocol at any time by pressing the STOP button Press QUIT SK to return to the main menu
28. ess TEST SK to check whether the plate alignment is centralised over the shaker or incubate position Note The numbers refer to steps approx 22 steps per 1mm The position 0 is to the front of the washer Increment the number in order to move the plate backwards Decrement the number if you wish the plate to move towards you If the plate is aligned press SAVE SK to scroll through to the next menu If the head is not aligned press CHANGE SK and repeat above steps This sets the length of time the head rests at the bottom of the wells In most cases the longer the head rests at bottom the drier the well will be left Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Use the up down keys to change the time The number is incremented in steps of 100 of a second You can choose from a minimum of 1 this equates to 100 of a second to a maximum of 255 this equates to 2 55 seconds Press TEST SK If the delay is correct press SAVE SK to scroll through to the next menu If the delay is not correct press CHANGE SK and repeat above steps PUMP SPEED CHANGE NEXT QUIT 20 5 255 TEST QUIT PUMP SPEED CHANGE SAVE QUIT S DDD TROUGH DEPTH CHANGE NEXT QUIT TROUGH DEPTH 120 TEST QUIT Ea W 00 GD TROUGH DEPTH CHANGE SAVE QUIT OD TROUGH
29. f EthylAl ormamid Component Material Acetone cohol e DMF Delivery tubes Tygon X L X Peristaltic pump tubes Santoprene R R R Level sensing tubes Tube PP R R R fittings Acetal Polyoxymethylene Delrin Du Pont R R R Reagent bottles PC X R X Bottle tops ABS Acrylonitrile Butadiene Styrene X R L X Dispense tubes Head lifting rods Screws R 2 Dispense head PPS Ryton polyphenylene sulfide R R R Nylon R R R Plate carrier Top plate PPS Ryton polyphenylene sulfide R R R Front and back mouldings ABS Acrylonitrile Butadiene Styrene X R L X X Not Recommended L Limited R Recommended 13 General Specification Dimensions main unit Weight complete system Operating voltage Power consumption Power Supply Environmental operating temperature Environmental storage temperature Precision 350ul Dispensing volume Residual volume Protocols Max number of protocols Max number of washes Min wash volume Max wash volume Min soak time Max soak time Min aspirate delay Max aspirate delay e Autura User Manual Page 37 of 48 230mm W X 485mm D X 275mm H 6 5Kg 100 240Vac 50 60Hz 51 6VA 12V 4 3A DC Switch Mode centre 10 C 40 C 4 C 40 C CV 3 300ul 25 1500Hl in 25ul increments 3ul per well typical action Tul per well with sweep 30 10 1500Hul 1 seconds 15 minutes 0 01 second 2 55 seconds The CE sign certifies that the instrument
30. h bottles with 10 bleach solution 5 Weta cleaning pad with bleach solution and clean all outside surfaces of the equipment and bottles 6 Pack the unit in its original packaging Mikura Ltd is not liable for any damage incurred in transport if the original packaging is not used Return amp Decontamination Certificate RAN number LTL TL L L L L1 Serial Number N BEH Emi Name Laboratory name Full address Street Town ZIP Tel No Fax No Email Laboratory supervisor Describe nature of fault Is the equipment s surface or components damaged in any other way mikura Mikura Limited Spinningwood Farm Burnthouse Lane Lower Beeding W Sussex RH13 6NN United Kingdom 44 0 1403 891875 44 0 1403 892651 info mikura co uk The equipment has been in contact through use or otherwise with the following media Body fluids Toxic media Carcinogenic media Radioactive media Please specify Other hazardous materials hereby certify that the enclosed equipment has been fully decontaminated as per the instruction in the Autura Manual To the best of my knowledge service personnel can handle the equipment without exposing them to health hazards Signed Print name Date 12 Materials compatibility chart Autura User Manual Page 36 of 48 Ethanol Dimethyl
31. hine is either delivered with pre programmed wash protocols or with a default sample protocol In both cases you can edit the protocols with ones of choice The control panel is equipped with a total of seven keys soft keys Soft keys are keys that are assigned to carry out a certain function These three keys are located adjacent to the bottom line of the display and its function is indicated on the display For the purpose of clarification soft key functions will be accompanied with SK in this manual ENTER key The enter key saves in some cases your selection from the screen into Autura s memory UP amp DOWN keys These keys will either increment or decrement the values on your setting or toggle between various setting options STOP key The STOP key allows you to terminate any protocol at any time Autura User Manual Page 13 of 48 5 Utilities 5 A Priming the washer Before you start to use the washer you should ensure that the wash solution delivery tubes that lead from the wash bottles through the peristaltic pump to the wash head are primed i e full with wash reagent and no air bubbles can be seen in the delivery tubes For this purpose Autura has a dedicated priming utility The following chart will run you through all the necessary steps to prime the tubes You can quit the protocol editing process at any stage by pressing the QUIT SK button PROTOCOL 1 30 1 UTI
32. ied with either a set of pre programmed protocols or with a single default protocol in all 30 positions The following chart will run you through all the necessary steps to run a protocol You can quit the running of a protocol at any time by pressing the STOP button PROTOCOL 1 30 1 UTILS RUN 1 30 1 UTILS RUN EDIT W d 42 PRIMING a ee Ge GD D GD START ROW 1 12 NEXT You have to determine which of the 30 protocols you would like to run Select the desired protocol by scrolling through using the up down keys Press RUN SK to run a protocol Autura starts the vacuum pump in order to build up the required vacuum in the waste bottle First time it will initiate a self priming cycle before it runs If you can still see air bubbles in the delivery tube you can prime again by pressing the PRIME AGAIN Sk or press RUN SK Choose the row you want to start from by scrolling through using the up down keys Then press NEXT SK Choose the row you want to end on by scrolling through using the up down keys Then press NEXT SK PROTOCOL 1 RUNNING RUN COMPLETE PRESS ANY KEY PROTOCOL REPEAT a PROTOCOL 1 30 1 UTILS EDIT Autura User Manual Page 16 of 48 A
33. meets the requirement of the EEC directive and has been tested according to the specified test methods WEEE COMPLIANCE This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol x Mikura Ltd has contracted with one or more recycling disposal companies and this product should be disposed of or recycled through them Further information on Mikura Ltd s compliance vvith these Directives the recyclers and information on Mikura Ltd products which may assist the detection of substances subject tothe RoHS Directive are available on request Produced by Mikura Ltd Spinningwood Farm Burnthouse Lane Lower Beeding W Sussex RH13 6NN England possible 4 It is the stated philosophy of Mikura Ltd to preserve the environment wherever Mikura will only use materials and production techniques that cause least environmental damage Autura User Manual Page 38 of 48 List of Protocols moq juabeay 1 adh 1u AZI p jqeu 1U9A2 dils JO lEld USEAA M pu ulonoq JO do USPAA jam SWNIOA USEAA l 1S M S USEAM JO ON 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 3
34. o scroll through to the next menu Use the up down keys to toggle between YES or NO Press SAVE SK to scroll through to the next menu Autura User Manual Page 46 of 48 15 Appendix B Hardware information for the Service Engineer Autura has a microprocessor of type Winbond W78E516B40PL This is located on the PCB behind the front cover It is programmable via the USB socket The PCB also contains an eeprom that is rewritten with new hardware settings through the engineering menus This eeprom is not replaceable 16 Appendix C Upgrading software via USB socket The USB Interface CD contains all the software required for uploading new software versions to the Autura 1000 washer from a PC running all versions of Windows 9X to Windows Vista To install the software copy the folder called PC Software from the CD to your local HD Quit all other applications Open the folder and create a shortcut for Autura memory loader to your desktop The Autura is equipped with a USB type B socket Connect your Autura Washer 1000 to your PC using an USB type A to type B cable i e printer cable Connect USB cable to Autura first then to the computer Switch on your Autura and launch the Autura memory loader software application from your desktop Autura Memory Loader Open File Initialise Click the Initialise button to start communication with your Autura The Autura display will read Memory Loader Mode and the sof
35. rvoir via a peristaltic pump This type of pump is used for this function so that you can optimise the delivery volume and pressure of the wash solution by adjusting the speed and rotor revolutions through the software Peristaltic pumps pulse the liquid with every revolution of the rotor and knowingly provide a very efficient washing system Each tip is constructed from concentric Stainless Steel tubes The liquid is pumped into the wells through the internal tube and aspirated via the external tube A pump is housed within the unit and produces a vacuum within the waste bottle The pump runs all the time the unit is carrying out a wash cycle to avoid the possibility of well to well overflow and reduce cross contamination The wash head height is set such that its top position will not allow overflow of liquid in the dispense position and leaves positive meniscus in the wells any danger of overfill is therefore eliminated Over dispensing has been found to be a very effective way of washing a plate i e dispensing an amount of liquid into the well which is larger than the well s capacity After plate washing Autura positions the wash head into the soak trough and dispenses a small amount of wash solution into the soak trough Autura User Manual Page 12 of 48 4 C Familiarising yourself with Autura system software interface components Autura stores up to 30 wash protocols in its memory According to the machine s specification the mac
36. s before moving it back under the wash head for the next wash cycle Press STORE SK to scroll through to the next menu Use the up down keys to toggle between the options Here you can choose which reagent you wish to use for this wash protocol This function is of use for those washers that have been configured with more than one peristaltic pump Choose between A B or C for any of up to three reagents Press STORE SK to scroll through to the next menu Store the protocol that you have just edited in any of the 30 positions The up down keys will step you from position to position The OVERWRITE SK will store the new protocol NOTE Storing a protocol in a particular position overwrites the protocol which was previously in that position Following the storage of a protocol Autura will return to display the main menu Autura User Manual Page 20 of 48 7 B Chaining protocols A simple way to achieve complex wash sequences is to chain together various protocols If chaining is enabled you can chain together up to four protocols Autura will run each chain as a single protocol characterised by your setting for each of the individual protocols according to any sequence you desire You can create up to 5 different chains PROTOCOL 1 30 1 Press UTILS SK in order to access the utilities menu UTILS RUN EDIT Select the Chain function by scrolling through using the up down keys UTILITY OPERATIONS Pr
37. s CHANGE Sk Use the up down keys to change the acceleration Press TEST SK to check whether the plate acceleration is correct If plate acceleration is correct press SAVE SK to scroll through to the next menu If the plate acceleration is not correct press CHANGE SK and repeat above steps Z AXIS SPEED CHANGE NEXT QUIT DDD Z AXIS SPEED 35 TEST Z AXIS SPEED CHANGE SAVE QUIT Z AXIS SPEED TEST QuiT Z AXIS SPEED CHANGE SAVE QUIT Z AXIS ACCELERATION CHANGE NEXT QUIT DDD Z AXIS ACCELERATION 16 TEST QUIT GD db G amp D Autura User Manual Page 42 of 48 Z AXIS SPEED is the speed in which the head moves along the up and down axis Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the head will move up and down Check whether the speed is correct If head speed is correct press SAVE SK to scroll through to the next menu If the head speed is not correct press CHANGE SK Use the up down keys to change the speed Press TEST SK to check whether the head speed is correct If head speed is correct press SAVE SK to scroll through to the next menu If the head speed is not correct press CHANGE SK and repeat above steps Z AXIS ACCELERATION is the acceleration in which the head moves along th
38. scroll through to the next menu m Bt Sas S ss 52535 s 2455 better H sss H s ett 245 Hiss 245 ey tet 7 s nd H SHAKER ENABLE Press CHANGE SK to enable the shaker or NEXT SK to scroll through to the next menu CHANGE NEXT QUIT SEO OOOO FE sesini AATETTA s re istisi s r r irs n is es Pitter 2222 22 Use the up down keys to toggle between YES or NO Press SAVE SK to scroll through to the next menu PE attest n 566 Bee Bitte 26 EEEN aes da HAS Bette HAAS bette 9526 Gitte 245 0 24 tte tates d Sse 2 d RRS SHAKER SPEED CHANGE NEXT SHAKER SPEED TEST QUIT y o W db Gb SHAKER SPEED CHANGE SAVE QUIT maz GD d GD SHAKER SPEED 248 TEST SHAKER SPEED CHANGE SAVE QUIT 5 ENABLE HEAD MOTOR NO SAVE Autura User Manual Page 44 of 48 This menu will not appear if you chose NO in the previous menu i e no shaker Press CHANGE SK to enter this procedure Press NEXT SK to scroll
39. sitioned to the right of the washer unit Conveniently place the remote power supply so the lead can easily be attached to the washer insure that it is not covered and has access to a free flow of air over it Autura User Manual Page 3 of 48 2 The socket panel The socket panel is located on the right hand side of Autura and contains the following USB socket for software upgrades Power On green LED which illuminates whenever the unit is switched on Power On Off switch Fuse Holder containing a F4A fuse Mini stereo socket for liquid level sensing from the wash bottle Mini stereo socket for liquid level sensing from the waste bottle A 5 0mm power socket that connects to the remote power supply Autura User Manual Page 4 of 48 3 Assembly 3 A General assembly 1 2 3 Control Panel Socket Panel Remove all packing material from the head and plate carrier area Plug the power supply cable into the socket on Autura s sockets panel Plug the mains cable into the remote power supply unit and the mains plug into your main supply Note that a green LED illuminates on the remote power supply unit indicating the correct operation of this component Do not switch on the unit at this time Fill the wash bottle with the chosen wash reagent and tighten bottle cap Connect the clear Tygon tubing from the longer outlet spigot on the bottle cap to the inlet bottom tube on the peristaltic pump Tighten
40. to check whether the head alignment is centralised over the first row of wells Note The numbers refer to steps approx 22 steps per 1mm The position 0 is to the front of the washer Increment the number in order to move the plate backwards Decrement the number if you wish the plate to move towards you If head is aligned central over the first row of wells press SAVE SK to scroll through to the next menu If the head is not aligned press CHANGE SK and repeat above steps This menu is designed to allow the front to back alignment of the plate in the shaker or incubate position Press CHANGE SK to enter this procedure Press NEXT SK to scroll through to the next menu Press TEST SK the plate will move into position Check whether the plate alignment is centralised over the shaker or incubate position SHAKER INCUBATE POSN CHANGE SAVE m SHAKER INCUBATE POSN 1401 TEST QUIT SHAKER INCUBATE POSN CHANGE SAVE QUIT ASPIRATE DELAY NEXT CHANGE QUIT ASPIRATE DELAY TEST Quit ASPIRATE DELAY CHANGE SAVE QUIT Autura User Manual Page 26 of 48 If plate is aligned central over the shaker or incubate position press SAVE SK to scroll through to the next menu If the plate is not aligned press CHANGE SK Use the up down keys to step the plate over the shaker or incubate position Pr
41. tware will display Initialisation success Click the Open File button and select the new XXX hex file to upload After sending the data the software will display File opened Ok End of data Click the Write Data button The new software will be uploaded to the Autura After the data has been sent and verified the software will be display Programming Successful The Autura will return to the main menu Click the Quit button to close the program Switch off your Autura and unplug the USB cable
42. ual Page 8 of 48 M3 set screws for left to right adjustment 3 Level alignment Level alignment denotes the level of the head to the horizontal plane Move the plate with the plate carrier under the wash head and lower the head until the tips nearly touch the flat portion of the plate To remedy any misalignment loosen the M3 socket head screw on the front of the wash head cradle and rotate the cam from behind using a small slotted screwdriver until the head is level When it is level retighten the M3 socket head screw on the front Optical flag reader adjustment screws 4 Left to right alignment with head motor Move the plate with the plate carrier under the head until the tips are aligned at the edge of the first row of wells Gently lower the head by pushing it down until the tips are level with the plate The head requires a realignment of its left to right axis if the tips do not align with the centres of the wells To remedy any misalignment lift head motor using the appropriate Allen key loosen the two M3 socket head screws on the optical flag reader move the optical flag reader left or right by the same amount the head is out of alignment retighten the screws The Autura will need to be switched on for the motor to adjust to the new position Autura User Manual Page 9 of 48 3 E Changing from 8 way head to 12 way head The plate clamping arm needs to be turned around Ae Oe eh Pivot pin Spring Arm stop
43. uick LIA 15 6 A Running a wash PlOlOCOl ix 15 7 Navigating through the 1 iii 17 7 A a 17 7B Chaining Ea EEA ari 20 Engineering IN GMS ccc T 24 B Error MOSSAGSS oa o terete vibes ces a bee ted eee NR Sevag aba 31 9 Maintenance 32 9 A nen tte eines eae d ede 32 9 B At the beginning of a working day 2 32 10 Troubleshooling ees a la h R si 33 11 Sending back for maintenance or sa aei iii iii iii ian 34 12 Materials 36 13 General Specification eniri e 37 14 Appendix A Service Engineer Menus 40 15 Appendix B Hardware information for the Service Engineer 46 16 Appendix C Upgrading software via USB 46 Autura User Manual Page 2 of 48 1 Unpacking the unit 1 A Unpacking Place the shipping carton on the floor
44. utura runs the selected wash protocol for the number of rows you have selected You can stop the process by pressing the STOP button on the control panel Autura confirms that the wash cycle is complete This message is to assure you that the cycle was not terminated prematurely Press any key to confirm Press REPEAT SK to repeat protocol or QUIT SK to return to main menu When you press QUIT SK Autura moves the priming trough under the wash head dispenses a pre set amount of wash solution into it and rests the head into the trough 7 Navigating through the control panel T A Editing wash protocols Autura User Manual Page 17 of 48 The following chart will run you through all the necessary steps to edit a protocol You can quit the protocol editing process at any stage by pressing the QUIT SK button Always press the STORE SK button to enter your selection into Autura s memory and move to the next screen Press STORE SK in order to move to the next screen even if you do not change any values Use the edit function to review the settings of any protocol stored NOTE If you quit a screen at any stage of the editing none of the changes you have made will be saved In order to save any of your changes to a protocol you must scroll to the last screen that asks you to overwrite or change the protocol position you want to change PROTOCOL 1 30 UTILS RUN UTILS ot PROTOCO
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