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ShellPa

1. CLEANED PRIOR TO USE 1 After the ShellPa is properly pressurized turn the knob on the Controller to adjust frequency from 1 120 cycles minute The Controller must read STOP to change the frequency The default setting is 60 cycles minute 2 Press the START STOP button to start or stop a run Turning the knob during a Run will change the display to show either time of current run or total time of run The current time is reset every time the run is STOP The total time will reset to zero when the power switch on the back panel of the Controller is turned OFF 3 Inthe Main Unit turn the Offset Screw CLOCKWISE to properly align chamber holder with support arms Depress the Unload levers on each side of the holder and then lift the chamber holder out of the Main Unit a Depending on the ShellPa model some models need to be pressurized connected to air compressor to be able to unload load the chamber holder from the Main Unit Four holes to align chamber holder with support arms of Main Unit Stretch Ratio Adjuster Four Unload Load levers 12 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 4 Place the chamber holder in a sterile biological safety cabinet Select the desired stretch ratio using the Stretch Ratio Adjuster Refer to Stretch Programs for details 5 To load the seeded stretch chambers ont
2. air pressure is highly recommended during operation 10 Guidelines Working Valv Closed Pressure 1 regulator _ Counter clockwise Lift up turn Lift up turn 2 regulator Clockwise Counter clockwise 3 4 Bar Push down Push down Air cock valve Counter clockwise Clockwise Waste valve Counter clockwise Clockwise menn yellow tube 2 Regulator DO NOT pressurize over 5 Bar for safe use lt 10 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 When operating the Main Unit for long periods of time at frequencies of 60 cycles minute 1Hz or faster lower the pressure to 2 Bar to reduce load on the air compressor Turn OFF Air Compressor and ShellPa 1 2 Turn OFF the Controller using the toggle switch CLOSE the air cock valve on the black tubing Remove the BLACK tubing from the Controller by pushing in the grey plastic washer surrounding the tubing This will release the tube CLOSE the 1 regulator by turning the valve CLOCKWISE OPEN the air cock valve on black tubing to release pressure Decrease in pressure will be indicated by the 2 regulator CLOSE the 2 regulator by lifting up the knob turn COUNTER CLOCKWISE until it stops turning push down to lock position Release pressure from the air compressor tank by OPENING the waste valve Turn the valve COUNTER CLOCKWISE Pre
3. stretch chambers on the square plastic dish and pour fibronectin solution into the stretch chambers so that the bottom of the chamber is covered with fibronectin solution C Incubate at 37 C for at least 30 minutes or up to 2 hours D Aspirate the fibronectin solution The chambers are ready to seed with cells 3 Coated chambers are ready to be seeded with cells Incubate the seeded chambers overnight The next day check cells for attachment to the chamber 4 Place the chambers into ShellPa Allow the cells to stretch a few minutes Check the cells to confirm cell attachment is maintained 14 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 7 Stretch Programs Main Unit Stretch Ratio Adjuster Stretch Distance Lock 6 mm __8__ ew 16mm_ 20 Controller po Frequeney n Default 60 cycles minute 1 120 cycles minute 15 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 8 Trouble Shooting lf system is not stretching check the following items All tubings and cables connected Air valve cock open First air pressure regulator valve is open Both the first and second air pressure regulators reading the correct pressure Both the Controller and air compressor are turned on and plugg
4. 200 Fax 1 408 252 6220 email customersupport b bridge com The information contained herein such as specification configuration and data or alike in part or in whole may be subject to change without notice ae B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200
5. 50 60Hz 0 3AOP DC12V Transformer For air compressor bd Rate ee D a A a LS SEVEN STAR OUTPUT 110V OR 220V Noise suppressor Plastic Square Dish covering chambers in Main Unit 13A NR Plasma treated 20mm x 20mm x 10 mm 4 attachment holes 3c B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 For seeding cells in stretch chambers and Disposable Stretch Chamber SC4B 3 Component Descriptions Main Unit Offset Knob For putting in or taking out Chamber Holder Plastic Square Petri Dish to seed cells in the stretch chambers and cover chambers in Main Unit Chamber Holder Holds 1 6 stretch chambers Button to open cover Stretch Ratio Adjuster Controller jo a Read out of experimental conditions Control Dial Turn dial to set stretch frequency Range is 1 cycle min to 120 cycles min The default value is 60 cycles min Start Stop Switch W B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 Back panel of Controller Connecting Port for AC Adapter Black Connecting Port Black tubing with air cock valve connects to air compressor Blue amp Red Connecting Port Blue amp Red tubing connects to Main Unit White Connecting Port White tu
6. bing with noise suppressor Power Button JUN AIR 3 4 MC Air Compressor 2 Air Pressure Regulator gauge and valve Power Switch BMF Integrator Mist Filter 5 u 1 u Connect to Controller via Black tubing with air cock valve 1 Air Pressure Regulator gauge and valve Single Phase 100V 0 15Kw Tank 4L Working Pressure 0 5MPa Switchback Pressure 0 7MPa 5 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 4 Set Up and Shut Down of Air Compressor and ShellPa AT THE POINT OF PURCHASE THE CHAMBER HOLDER IS LOADED ON THE MAIN UNIT THE MAIN UNIT MUST BE PRESSURIZED TO UNLOAD THE CHAMBER HOLDER READ THE ENTIRE USER MANUAL BEFORE USING SHELLPA OR ATTEMPTING TO ASSEMBLE OR DISASSEMBLE THE INSTRUMENT Set up air compressor 1 Take off the yellow and black protective cap on the side of the air compressor and replace it with the intake air filter 2 Screw the BMF Integrator Mist Filter 2 Air Pressure Regulator into the 1 Air Pressure Regulator Tighten the adjoining screw 1 air pressure regulator valve BMF Integrator Mist Filter gt 3 Make sure all the valves are in the CLOSED position a First air pressure regulator turn CLOCKWISE to close b Second air pressure regulator pull up knob turn COUNTER CLOCKWISE to close push down knob to lock position c Waste
7. d A Shell Pa Standard Mechanical Cell Stretch System Model No NNMS Serial User Manual To operate the system properly and safely read the manual before using ShellPa This system is not a medical device For RESEARCH USE ONLY Manufactured by y Menicon Life Science B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 Table of Contents We WIP OCUIC WO Miss ii rai p deci ti ay FAT E a si Ok ae E e l FF kep one FFF FY FEN FFAN 2 2 POMC UPS COMO ONC INS scene antr kr di ns kato iv ta ik atis e n e e e e nn AU Dy YF aoe DOW a a 2 3 COMPONENT D SCNP WO FI vois sa zak aa aa l a kt e a e a ak a l a j ae e an n bn bale beke OD di bn 4 4 Set Up and Shut Down of Air Compressor and ShellPa cc ccc cece ee LL LL LL LL LL Y iun 6 du OPEN OF SiC se SEE FEN TR FN FFR FA NW T a 12 6 Coating and Seeding Chambers 99 9YYYY Y aaa RL RL RR Y HL RY RR LY LY LY Lwy 14 fe CLCH TO TO a GO RODO GELE a i ene n ee OOED DO AUU DEU 15 8 Troubleshooting 9 YYS YL anana LL a aaa taa taesettesteseseoeseoesteseseoeeeeoseseeoesooeseneon 16 di VYANN ANY LIY HT ota tk tite ti tk ia an lt aa ANF E td kn pi e a ei ek en a FYFYR e a ADAR 17 A Safety Precautions In case of misuse of ShellPa it may lead to a risk of severe injury and or breakdown of the system Only use Shel
8. ed into an appropriate power source Power source on Stretch Ratio Adjuster is not in the lock position 0 stretch ee B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 Warranty Model No NNMS001 1 The warranty is for one year commencing the date the customer receives the product and includes the instrument casing and non wearable parts The stretch chambers are considered consumables and are only replaced if received damaged or found defective 2 The warranty does not cover damage to the instrument that is a result of the following circumstances 1 Damage caused by dropping or other impact 2 Damage caused by inappropriate operation of the instrument 3 Damage resulting from an attempted repair or modification of the instrument by the user 4 Damage caused by unavoidable external causes such as earthquakes lightening fire flood gas leak power surges or other acts of providence 3 B Bridge International Inc is free from any responsibility for effects or loss or damages arising from the result of the machine operation This warranty assures that B Bridge International Inc will repair our product free of charge as stipulated in our warranty policy Any shipping charges will be born by buyer B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 USA Phone 1 408 252 6
9. er surrounding the tubing then pull out the tubing 2 Connect the White tubing with the noise suppressor to the White port on the back panel of the Controller 3 Connect the Black tubing with air cock value to the Controller The air cock valve should be closer to the Controller and not the air compressor The air cock valve should be in the CLOSED position Attach the other end of the tubing to the BMJ Integrator Mist Filter on the air compressor Air cock valve Side view of BMJ Integrator Mist Filter Eti Or Open Closed Attach Black tubing to BMJ 8 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 4 Plug the AC Adapter into the Controller The AC Adapter is made for both 110 and 240 volts therefore no transformer is needed Plug the transformer with air compressor and AC Adapter into a 240 volt power source Turn ON Air Compressor and ShellPa CAUTION This system is pneumatic The misuse of the system may lead to a risk of breakdown Incomplete connections may cause an air leakage Be sure to confirm the proper connections before operating the system 1 Turn ON Air Compressor Power Switch The green light next to intake air filter will illuminate 2 OPEN the valve of 1 regulator by turning the valve COUNTER CLOCKWISE The pressure will build to 7 Bar on the regulator When
10. lPa with the an air compressor Turn OFF power when not in use Read the entire ShellPa manual before assembling and using the instrument Read the operating manual for JUN AIR air compressor as well Only use the designated air pressure Using other values may lead to damaging the instrument Keep away from flammable agents Do not touch with wet hands Instrument and air compressor should not be operated in standing water ie B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 1 Introduction ShellPa was designed to replicate the dynamic cellular environment of the body by applying mechanical strain to cultured cells Physiological cell response to mechanical stress is a more realistic model than conventional static cell culture 2 ShellPa Components Main Unit Controller D N m al Start Stop y Menicon j Life Science Air compressor Intake air filter with BMF integrator mist filter and 2 air pressure regulator attached BMF Integrator Mist Filter and 2 Air Pressure Regulator LO B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 Red and Blue Air Tubes Black Air Tube with air cock valve White Air Tube Exhaust tube with noise suppressor AC Adapter Model US301210 AC100 240V
11. o the chamber holder turn Offset Screw COUNTER CLOCKWISE to release tension and allow the chambers to easily slide on and off the metal pins Once 6 chambers are all set turn the Offset Screw CLOCKWISE to put holding tension on the chambers 6 Use the plastic square dish lid or bottom to protect the chambers The lid is held in place by the 4 fixed pins toward the bottom edge of the chamber holder Push the edge of the lid between the 4 pins 4 fixed pins to hold plastic dish 7 Load the chamber holder back into the Main Unit B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 8 The entire Main Unit can be placed in a cell culture incubator during use THE MAIN UNIT CAN BE PLACE IN INCUBATORS WITH TEMPERATURES RANGING FROM 10 40 C AT 95 HUMIDITY THE CONTROLLER SHOULD REMAIN ON THE OUTSIDE OF THE INCUBATOR INA DRY CLEAN ENVIRONMENT THE AIR COMPRESSOR SHOULD BE OPERATED INA DRY CLEAN ENVIRONMENT AND ON A LEVEL AND STRONG PLATFORM OR FLOOR 6 Coating and Seeding Chambers 1 Stretch Chambers are disposable and are sold ready to use They are ethylene oxide gas sterilized 2 Chambers should be coated with an extracellular matrix like fibronectin prior to seeding with cells Example coating procedure A Dilute fibronectin to a final concentration of 2 20ug mL in Phosphate Buffered Saline PBS B Place the
12. ssure will decrease in 1 regulator The air compressor motor may automatically turn on Leave the motor ON until the fluid collected in the air pressure tank is flushed out the waste tubing a The air compressor compresses the atmospheric air and absorbs the moisture in the air that causes the fluid to pool in the tank A periodical fluid removal from the tank drain and mist filters is required In case of interfusion of such a fluid into the main unit or and controller may lead to a risk of breakdown of the system Once pressure in 1 regulator reads zero and waste fluid is expelled turn OFF motor Blot the bottom of the BMJ filters with an adsorbent towel CLOSE the waste valve by turning CLOCKWISE Unplug the Controller and air compressor from the power source Unplug the tubes red blue white and black with air cock valve from the Main Unit Controller and air compressor by pushing in the grey washer which will release its grip on the tubing 11 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 13 When transporting the air compressor or if not using for extended period of time replace the intake air filter with the yellow protective cap to prevent oil leakage and dust interfusion 5 Operation of ShellPa DISINFECT THE SHELLPA WITH 70 ETHANOL WIPES ALL SURFACES THAT POTENTIALLY CAN HARBOR CONTAMINANTS SHOULD BE
13. the pressure reaches 7 Bar the compressor will automatically turn off and the green light will also turn off a The regulator will indicate pressure regardless of the valve being open or closed Therefore the measurement of pressure in the regulator is not necessarily an indication that the valve is open 1 regulator at 7 Bar SO B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 3 OPEN the 2 regulator by lifting the knob up then turning it CLOCKWISE Continue to turn the knob until the regulator reaches 3 4 Bars 4 Once 2 regulator reaches the desired pressure lock the position by pushing down on the knob 2 regulator at 3 Bar 5 OPEN air cock valve on black tubing leading to Controller This will pressurize the ShellPa 6 When the air pressure in the 1 regulator lowers to 5 Bar the compressor will automatically turn on until it pressurizes to 7 Bar then the compressor will turn off 7 Turn ON the Controller using the toggle switch on the back panel 8 Turn the knob on the Controller to adjust frequency Press the START STOP button to start or stop a run a The Controller must read STOP to change the frequency Default setting is 60 cycles minute b Turning the knob during a Run will change the display The knob will toggle between current run and total run time 9 Routine checking of
14. valve turn CLOCKWISE to close 7 Waste value 6 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 4 Make sure both drain tubes thick yellow tube and black tubing drain into a waste container The small black tubing may already be connected to a plastic bottle Waste tubes 5 Check oil level in the Air Compressor If oil is below the minimum MIN level add SJ 27 oil through the oil inlet F WN AIR q E w Oil inlet 6 THE COMPRESSOR IS MADE FOR 110 VOLTS AND NEEDS A STEP DOWN TRANSFORMER IF USING A 220 240 VOLTS POWER SOURCE PLUG THE AIR COMPRESSOR INTO AN ELECTRICAL TRANSFORMER INCLUDED WITH THE TRANSFORMER IS A PLUG ADAPTER 27 2 B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 www b bridge com customersupport b bridge com 1 408 252 6200 Set up Main Unit and Controller Depending on the ShellPa model the location and orientation of the ports blue red white black may be different than the image below For all models the color of the port corresponds to the color tubing it connects to Back panel of Controller Back or side panel of Main Unit 1 Use the Blue and Red tubing to connect the Main Unit to the Controller by gently pushing the tubing into the same color port To release the tube from the port push in the grey plastic wash

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