GH-3.8 Swinging Bucket Rotor
Contents
1. Figure 13 Lubricating Pivot Pin Bucket Contact Areas Do not use any type of lubricant or grease on the threads of the drive shaft e Refer to publication TJ6 TB 011 for information on Aerosolve cannister maintenance Refer to Chemical Resistances publication IN 175 for the chemical compatabilities of rotor and tube materials Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center CLEANING gt NOTE Do not wash the rotor components or accessories in a dishwasher Do not soak components in detergent solution for long periods such as overnight Do not autoclave the tie down nut GH 3 6 Rotor The Rotor Components e fspillage has occurred or if salt solutions or other corrosive materials are used clean the rotor and components immediately Do not allow corrosive materials to dry on rotor components Approximately once a week or every 1000 runs clean the pins and bucket pin sockets then lubricate as described under MAINTENANCE Under normal conditions remove the rotor from the centrifuge at least once a month for cleaning and lubrication to prevent buildup of residues Use a mild detergent such as Beckman Solution 555 339555 diluted 10 to 1 with water and a soft brush to clean the rotor yoke and tie down nut The Rotor Cleaning Kit 339558 contains two quarts of Solution 555 and brushes that will not scratch the rotor Thoroughly rinse the2. Modular disk adapters and tube racks for Aerosolve cannisters are sold in weight matched sets Mark sets of adapters so that they can be easily identified When cleaning adapters do not mix sets Check the weight of adapters prior to use DoNOT intermix adapters or their components from Beckman Coulter s J6 series rotors with those used in the GH 3 8 rotor These adapters are similar in appearance see Figure 5 but have very different weights and functional characteristics Mixing them will cause centrifuge imbalance and potential instrument damage OPERATION SPEED DERATING The maximum allowable run speed 3750 rpm listed in the rotor specifications is for operation when all conditions are within the standard specifications using four buckets Maximum allowable run speed for the GH 3 8 rotor must be reduced in any of the following circumstances Maximum allowable run speed when Micro Plus plate carriers are used is 3250 rpm If two buckets and two carriers are used reduce run speed to the speed allowed for carriers Ifthe weight of the load in a bucket exceeds 1000 grams or if the solution density is greater than 1 2 g mL use the following square root reduction formula to determine the allowable rotor speed 1000 grams d 3750 M 1 pee ipao heaviest load in grams 0 Ifthe weight of the load in a Micro Plus multiwell plate carrier exceeds 500 grams including base pad labware and sample use the following sq
3. pkg 24 96 well nonsterile Aluminum foil lid 538619 pkg 100 Cap strip nonsterile 267002 pkg 10 Deep well 267007 polypropylene plate 1 mL well Cap strip sterile 267005 pkg 10 pkg 24 96 well sterile Aluminum foil lid 538619 pkg 100 Square well 2 mL well 140504 Aluminum foil lidt 538619 pkg 100 polypropylene plate pkg 24 Microfuge tube 1 5mL 357448 Rack insert white 373696 373661 polyallomer clear pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356090 Rack insert white 373696 373661 polypropylene clear pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356091 Rack insert white 373696 373661 polypropylene blue pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356092 Rack insert white 373696 373661 polypropylene green pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356093 Rack insert white 373696 373661 polypropylene yellow pkg 500 pkg 25 holds 24 tubes Microfuge tube 1 5 mL 356094 Rack insert white 373696 373661 polypropylene orange pkg 500 pkg 25 holds 24 tubes Caps are optional Requires soft rubber roller 4 in part number 538618 for installation GH 3 6 Rotor Figure 2 Rotor Yoke and Centrifuge Drive Shaft Be sure the pin in the drive shaft is in the groove in the rotor yoke 3 When the yoke is seated ensure that the rotor tie down nut O ring 927571 is lubricated in good condition
4. LOW brake setting Table 7 Approximate Acceleration and Deceleration Times for the GH 3 8 Rotor in GP Series Centrifuges Acceleration Deceleration H Deceleration L Instrument seconds seconds seconds 60 Hz GP or GPK 165 85 120 60 Hz GPR or GPKR 140 80 125 50 Hz GP or GPK 180 105 120 50 Hz GPR or GPKR 150 85 130 H HIGH brake setting L LOW brake setting gt NOTE Line voltage fluctuations can cause variations in acceleration and deceleration times The times listed in Tables 6 and 7 were achieved with line voltage ratings at 120 volts for 60 Hz instruments and 230 volts for 50 Hz instruments gt NOTE Do not use high brake when Micro Plus plate carriers are run as the force of the deceleration will disturb the contents of the plate Set the centrifuge brake switch to either LOW or OFF to coast to a stop 32 GH 3 6 Rotor CARE AND MAINTENANCE gt NOTE Accumulations of dust or other foreign matter can damage rotor pins resulting in rotor vibrations To prevent this clean the centrifuge bowl daily Spray the centrifuge bowl with an antistatic solution and wipe it clean INSPECTION Periodically at least monthly inspect the rotor yoke buckets and or multiwell plate carriers especially inside cavities for rough spots or pitting white powder deposits frequently aluminum oxide or heavy discoloration If any of these signs are evident do not run the rotor Contact your B
5. 0 961648 silicone vacuum grease 202 1 2 ec eee eee eens 335148 Micro Plus multiwell plate carriers includes bases and pads set of 2 362394 Micro Plus replacement carriers set of 2 lees 361304 Replacement bases for Micro Plus carrier includes bases and pads set of 2 361302 Replacement pads for Micro Plus carrier set of 4 0 00000 362390 SUPPLIES Modular disk adapters polypropylene 0 cee eee eee eee see Table 2 Tubes and bottles einet ie reme EES BARE EE as a eee NA see Table 3 Micro Plus labWates oe recor ox acs Wika RR as he OUR a ets UK see Table 4 Soft rubber roller for aluminum foil lids 0 0 0 0 000 cece eee 538618 Torquing bar Rr IRR a wil Shaw ites RR eg tat 356036 Tube decanter for use with blue adapter 0 0 eee eee eee 343108 includes gasket for 10 mm dia tubes silicone 0 0 c ee eee eee eee 343106 gasket for 12 mm dia tubes silicone lesse eese 343107 Adapter plate 1 5 mL for use with blue adapter 0 000 354511 Aerosolve cannister kit set of 4 each kit includes cannister 1 lid 1 O ring silicone vacuum grease and 1 pad 0000 359232 Aerosolve cannister O ring ethylene propylene 04 345366 Aerosolve pad placed beneath cannister in bucket 0004 361269 Tube racks for Aerosolve cannisters 0 eee eee eee ee
6. All parts must be accompanied by a note plainly visible on the outside of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office 39 GH 3 6 Rotor SUPPLY LIST Contact Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed on the back cover of this manual or see the Beckman Coulter Benchtop Rotors Tubes amp Accessories catalog BR 9742 available at www beckmancoulter com for detailed information on ordering rotors tubes and accessories For your convenience a partial list is given below REPLACEMENT ROTOR PARTS Rotorassembly i senes eb EEUU E Lege ER 360581 Tie dOoWn nut zm DU ENSE ae igs eG ee fees eee E e UNS e USE T 366642 O ring Buna N rubber 20 0 eect eee eens 927571 Anodized aluminum buckets set of 4 20 0 0 0 ccc eee 360589 Bucket cover kit set of 2 2 0 ccc teen e 360585 each set includes cover assembly cose powestbe bagi E pU TELE peus tier eng 360586 O ring ethylene propylene
7. can be imagined as a line extending across the bucket or carrier from one pivot pin to the other If a bucket or carrier is loaded so that its weight is unequally distributed on either side of its pivotal axis it will not hang vertically at rest and more importantly may not swing to a horizontal position during a run see Figure 6 As a result extra stress will be placed on the bucket carrier tubes and or multiwell plates during the run increasing the possibility of breakage or rotor imbalance GH 3 6 Rotor AT REST AT SPEED re re Proper Weight Distribution Improper Weight Distribution Figure 6 Examples of the Effects on the Horizontal Swing of Buckets Caused by Proper and Improper Weight Distribution 21 GH 3 6 Rotor 22 USING BUCKETS You can load buckets before or after they are installed on the rotor yoke In either case we recommend filling the appropriate labware first and then loading the labware into the buckets This is especially important when using blood bags you can trip the imbalance detector in the centrifuge by pushing blood bags into cups within buckets installed in the rotor Refer to Tables 2 and 3 to determine the appropriate labware required for your application Whether you are running tubes bottles or blood bags you must load the buckets symmet rically around the center of rotation and each bucket must be loaded symmetrically with respect to its pivotal axis see SYMMETR
8. 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 01494 441181 Fax 01494 447558 e mail beckman_uk beckman com Authorized dealers in other countries 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 Internet www beckmancoulter com Telex 678413 Fax 1 800 643 4366 CA S Printed on recycled paper
9. 2700 3500 green B D Vac RB plain 15 16 x 130 2900 3550 green Corex 8060A LT 15 17 x 120 max 3750 green Corex 8080A LT G 15 17 x 120 max 3750 green Corex 8442 RB 15 16x 115 max 3750 green Corvac RB SB 15 16 x 131 max 3750 green Kimax LT G 15 17 x 118 3000 3600 green Pyrex 8500 ST 15 17 x 110 2700 3500 green Pyrex 8080 LT G 15 17 x 120 max 3750 green Pyrex 8320 ST 40 29 x 116 max 3750 yellow Kimax 45212 ST 50 29 x 11 1000 2100 yellow Pyrex 8280 ST 50 29 x 11 2500 3300 yellow Most common usage Code B D Becton Dickinson Dispo disposable FB flat bottom G graduated LT long taper RB round bottom SB silicone gel barrier SC screw capped The following are registered trademarks Corvac Sherwood Medical Industries Inc Pyrex and Corex Corning Glass Works Kimax Kimble Division of Owens Illinois SST and Vacutainer Becton Dickinson and Company ST short taper Vac Vacutainer B D GH 3 6 Rotor 31 GH 3 6 Rotor Table 6 Approximate Acceleration and Deceleration Times for the GH 3 8 Rotor in Allegra 6 Series GS 6 Series and Spinchron Series Centrifuges Acceleration Deceleration H Deceleration L Instrument seconds seconds seconds 60 Hz Nonrefrigerated Centrifuge 160 80 110 60 Hz Refrigerated Centrifuge 140 80 110 50 Hz Nonrefrigerated Centrifuge 160 80 110 50 Hz Refrigerated Centrifuge 160 80 110 H HIGH brake setting L
10. 356985 Cannister Kit 500 183 2880 359481 359232 4 Tube retainers and adapter plates are sold individually Continued b Tube racks used with Aerosolve cannisters do not provide full tube support some manufacturers plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks Beckman Coulter highly recommends that you pretest other manufacturers tubes in the appropriate Aerosolve cannister labware using water samples c Cannisters and lids are made of polyphenylsulphone O rings are ethylene propylene Cannister kit includes the pad that must be used beneath the cannister in the GH 3 8 rotor sold in sets of two or four 4 Corning polypropylene bottle Light purple adapter replaces the previous tan adapter See note under SYMMETRIC AND BALANCED LOADING for weight difference information GH 3 6 Rotor Table 2 Modular Disk Adapters Bottle Adapters and Cups Available for the GH 3 8 Rotor continued BOTTLE ADAPTERS polypropylene Nominal Nominal Maximum Tmax at RCF at Bottle Bottle Number Bottle Maximum Adapter Color Volume Diameter Bottles Bottom Speed Part Number Code mL mm in Rotor mm x g qty one orange conical 230 62 4 195 1 3070 356983 use with 349946 yellow 250 62 4 195 1 3070 349946 warm red conical 2504 62 4 203 2 3200 349849 light purplee 50
11. Adapter Rotor mm x g Adapter Two Four blue 3 10 37 148 184 7 2910 5 359469 359148 5 12 tan 3 amp 13 30 120 184 7 2910 5 359478 359157 5 orange 7 amp 14 24 96 184 7 2910 6 359470 359149 10 purple 12 16 19 76 184 7 2910 7 359471 359150 green 15 18 14 56 194 7 3070 6 359472 359151 conical green 15 amp 18 14 56 184 7 2910 7 359473 359152 20 It green 30 amp 30 4 16 191 3010 5 359475 359154 conical 50 yellow 50 29 7 28 184 7 2910 6 359474 359153 dk blue 50 35 4 16 184 7 2910 7 359476 359155 brown 100 44 2 8 184 7 2910 3 359477 359156 tube 3 10 37 148 1 3431082 decanter 5 12 each 1 5 mL 1 5 amp 11 26 104 1 35451 12 adapter 1 8 each plate a Tube retainers and adapter plates are sold individually Continued b Tube racks used with Aerosolve cannisters do not provide full tube support some manufacturers plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks Beckman Coulter highly recommends that you pretest other manufacturers tubes in the appropriate Aerosolve cannister labware using water samples c Cannisters and lids are made of polyphenylsulphone O rings are ethylene propylene Cannister kit includes the pad that must be used beneath the cannister in the GH 3 8 rotor sold in sets of two or four 4 Corning polypropylene bottle Light purple adapter replaces the previous tan adapter See note under SYMMETRIC AND BALANC
12. cleaned rotor and components with water and air dry upside down Do not use acetone to dry the rotor When the rotor is dry lubricate the bucket sockets and pins as described under MAINTENANCE Before reinstalling the rotor lightly lubricate the drive hole of the rotor with Anti Seize 961660 to prevent the rotor from sticking as follows 1 Apply the lubricant onto a swab 2 Draw the coated swab through a paper towel to remove excess lubricant 3 Lightly coat the inside of the drive hole with the lubricant remaining on the swab Buckets 1 Remove the O ring from the bucket before cleaning 2 Clean the bucket and O ring with a mild detergent such as Solution 555 339555 diluted 10 to 1 with water then rinse thoroughly and dry completely 3 Coat the O ring with a light film of silicone vacuum grease 335148 Place the O ring on the ledge inside the bucket After cleaning lubricate bucket pin sockets with Paint On Graphite Lubricant 977212 as described under MAINTENANCE Micro Plus Plate Carriers Including Base and Pad Blood Bag Cups and Bucket Covers Wash with a mild detergent such as Solution 555 339555 diluted 10 to 1 with water and a soft brush Thoroughly rinse them and air dry upside down 35 GH 3 6 Rotor 36 Modular Disk Adapters 1 To disassemble adapters for washing first pull the bail out of the groove in the disks then remove the disks and unsnap the bail from the rubber bottom 2 Use a
13. do not run the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center To reduce the potential for corrosion clean buckets or carriers thor oughly immediately following a tube or well plate breakage Be sure to remove all glass particles from buckets or carriers Components or accessories designed for other rotors may cause rotor mishap if used in this rotor Use only components and accessories that have been designed for use in this rotor 7he safety of rotor components and accessories made by other manufacturers cannot be ascertained by Beckman Coulter Use of other manufac turers components or accessories in the rotors may void the rotor warranty and should be prohibited by your laboratory safety officer If tubes microplates or other labware made by manufacturers other than Beckman Coulter are used reduce rotor speed to prevent breakage The strength of glass and plastic tubes can vary between lots and will depend on han dling and usage we highly recommend that you pretest labware in the rotor using water samples to determine optimal operating conditions Scratches even micro scopic ones significantly weaken glass tubes Do not substitute a metal fastener for the plastic tie down nut supplied with the rotor If a tie down nut comes off the shaft during centrifugation the plastic nut furnished will break apart in the chamber causing minimal d
14. matched sets of adapters together and to weigh other adapters to be sure they are approximately the same Marking matched sets will help you keep them together gt NOTE The light purple 500 mL adapters 349945 replace the previous tan adapters There is a weight differ ence between the old tan adapters and the current purple adapters If you run a mix of the purple and tan adapters be sure to run matching colors in opposing positions or rotor imbalance could occur It is not necessary to completely fill all tubes positions in adapters or wells in multiwell plates however partially filled adapters or multiwell plates must be balanced with respect to the pivotal axis of the bucket or carrier as discussed below In adapters each tube should be placed so that its weight is balanced by a tube in a diametrically opposite position across the pivotal axis in the same adapter see Figure 4 Adapters placed in opposing buckets should also be filled the same way If you must run only one tube in an adapter be sure this tube rests over the bucket s pivotal axis Use the center hole of the adapter if one is available Use a side hole in line with the bails vertical supports 19 GH 3 6 Rotor 20 Turn the adapter so that the tube rests across the pivotal axis of the bucket The bails will no longer be in line with the pivotal axis check that they do not interfere with the proper swing of the bucket gt NOTE Be sure t
15. mild detergent such as Solution 555 339555 diluted 10 to 1 with water and a soft brush to scrub the adapters 3 Rinse and dry then reassemble 4 f necessary apply a light film of silicone vacuum grease 335148 to the grooves on the rubber bottom to make reassembling easier Keep matched sets of adapters together do not intermix sets Aerosolve Cannisters Refer to publication TJ6 TB 011 DECONTAMINATION If the anodized aluminum buckets or carriers become contaminated with radioactive material decontaminate them using a solution that will not damage their anodized surfaces Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash 4 Beckman Coulter does not however warrant the performance of these products with respect to their effect on the carriers or their ability to decontaminate these parts If the rotor or other components are contaminated with toxic or pathogenic materials follow appropriate decontamination procedures as outlined by your laboratory safety officer STERILIZATION AND DISINFECTION The rotor yoke buckets tube adapters and aluminum parts of multiwell plate carriers can be autoclaved at 121 C for up to an hour Blood bag cups bucket covers and Aerosolve cannisters purchased after April 1993 see the date code on the cannister bottom can be autoclaved at that temperature for about 1
16. with potentially hazardous materials open cannisters in an appropriate hood or biological safety cabinet in case of tube breakage If a glass tube breaks in an Aerosolve cannister tube rack discard and replace the O ring and rubber tube cushion in the base of the rack Imbedded glass particles that remain in the bucket or adapters can break tubes during subsequent runs 5 Flammability hazard Do not use in or near operating centrifuges 37 GH 3 6 Rotor 38 SST or Corvac Tubes If an SST or Corvac tube breaks the tube s silicone gel barrier material becomes laced with glass fragments and the tube contents contaminate the entire tube adapter and bucket or tube rack and cannister If one of these tubes breaks in an adapter in a bucket use the following procedure 1 Fill the bucket with water 2 Autoclave the entire bucket and contents at 121 C for 20 minutes 3 Decant any remaining water and dislodge the tube adapter do not remove it over a waste container 4 Working under a hood and using plastic gloves immerse the bucket and tube adapter intact in a 2 liter beaker or similar vessel containing ethyl acetate 5 Scrub the adapter with a brush and decant the ethyl acetate mixture into a waste bottle for proper disposal 6 Rinse with water and air dry the bucket and tube adapter If one of these tubes breaks in an Aerosolve cannister use the following procedure 1 Working in a biologic safety cabinet and wearin
17. 0 70 4 200 2 3150 349945 blue 750 96 4 195 2 3070 349846 Tube retainers and adapter plates are sold individually b Tube racks used with Aerosolve cannisters do not provide full tube support some manufacturers plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks Beckman Coulter highly recommends that you pretest other manufacturers tubes in the appropriate Aerosolve cannister labware using water samples Cannisters and lids are made of polyphenylsulphone O rings are ethylene propylene Cannister kit includes the pad that must be used beneath the cannister in the GH 3 8 rotor sold in sets of two or four 4 Corning polypropylene bottle Light purple adapter replaces the previous tan adapter See note under SYMMETRIC AND BALANCED LOADING for weight difference information Table 3 Beckman Coulter Tubes and Bottles for the GH 3 8 Rotor Adapter Volume Part Dimensions mL Description Number Set of Two Set of Four OPEN TOP TUBES 16 x 76 mm 10 polyallomer 355640 349471 359150 3594842 3591622 16x 76 mm 10 polycarbonate 355630 359471 359150 3594842 3591622 16 x 76 mm 10 stainless steel 301108 359471 359150 3594842 3591622 18 x 98 mm 15 polycarbonate 342080 359473 359152 18 x 98 mm 15 polyethylene 342081 359473 359152 18 x 98 mm 15 polypropylene 342082 359473 359152 a Adapter used in Aerosolve cannister part number 359232 Continued b Tube retainers and adapter plates are sold ind
18. 08 20060 Cassina de Pecchi Milano Italy Telephone 02 953921 Fax 02 95392264 JAPAN Beckman Coulter K K TOC Ariake West Tower 2 5 7 Ariake Koto ku Tokyo 135 0063 Japan Telephone 03 5404 8359 Fax 03 5404 8436 MEXICO Beckman Coulter de Mexico S A DEC V Avenida Popocat petl 396 Colonia Gral Pedro Maria Anaya Codigo Postal 03340 Mexico D F Mexico Telephone 52 55 9183 2800 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 0297 230630 Fax 0297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Telephone 65 6339 3633 Fax 65 6336 6303 SOUTH AFRICA SUB SAHARAN AFRICA Beckman Coulter Stand 1A Primegro Park Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 e mail beckman intekom co za 2007 Beckman Coulter Inc SPAIN Beckman Coulter Espa a S A C Caleruega 81 28033 Madrid Spain Telephone 91 3836080 Fax 91 3836096 email bioresearch espana beckman com SWEDEN Beckman Coulter AB Archimedesvaegen 7 Box 111 56 SE 168 11 Bromma Sweden Telephone 0 8 564 85 900 Telefax 0 8 564 85 901 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 0800 850 810 Fax
19. 5 minutes Do not autoclave the plastic tie down 3 In U S A contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo In U S A contact Biodex Medical Systems Shirley NY internationally contact the U S office to find the dealer nearest you GH 3 6 Rotor nut or Aerosolve cannisters purchased prior to April 1993 Ethanol 7096 may be used on all rotor components including those made of plastic Refer to publication IN 175 for other chemical resistances of tubes bottles and accessories gt NOTE IsoClean can cause fading of colored anodized surfaces Use it only when necessary and remove it promptly from rotor surfaces While Beckman Coulter has tested these methods and found that they do not damage components no guarantee of sterility or disinfection is expressed or implied When steril ization or disinfection is a concern consult your laboratory safety officer TUBE BREAKAGE N CAUTION To reduce the potential for corrosion clean buckets or carriers thoroughly immediately following a tube or well plate breakage If a glass tube breaks remove the glass very carefully from the adapter and bucket Imbedded glass particles that remain in the bucket or adapters can cause tube failure during subsequent runs Aerosolve Cannisters N WARNING When working
20. 6983 polypropylene use w 349946 62 x 136 mm 250 polycarbonate 355673 3499465 with screw cap round bottom a Adapter used in Aerosolve cannister part number 359232 Continued b Tube retainers and adapter plates are sold individually These tubes cannot be run with bucket covers in place 4 Do not load the 750 mL bottle directly into the bucket always use the adapter Replacement bottle cap part number is 356263 set of 6 11 GH 3 6 Rotor Table 3 Beckman Coulter Tubes and Bottles for the GH 3 8 Rotor continued Adapter Volume Part Dimensions mL Description Number Set of Two Set of Four BOTTLES continued 62 x 122 mm 250 wide mouth 356013 349946b polycarbonate with cap 62 x 122 mm 250 wide mouth 356011 349946 polypropylene with cap 62 x 122 mm 250 wide mouth 358275 3499465 polycarbonate 62 x 120 mm 250 wide mouth 358326 3499465 polypropylene 69 x 160 mm 500 polypropylene 355607 349945 gt with cap assembly 69 x 159 mm 500 polypropylene 355665 349945 gt with cap 69 x 159 mm 500 polypropylene 355650 3499455 96 x 130 mm 750 polycarbonate 3582994 3498465 with screw cape 96 x 130 mm 750 polypropylene 3568554 3498465 with screw cape a Adapter used in Aerosolve cannister part number 359232 b Tube retainers and adapter plates are sold individually These tubes ca
21. Before the first use of a new rotor lubricate the rotor pins and bucket pin sockets as described under MAINTENANCE Also remove the lubrication decal over the rotor drive hole in the yoke and lubricate the inside of the drive hole as described under MAINTENANCE 1 Note the orientation of the pin on the drive shaft and the groove in the bottom of the rotor yoke see Figure 2 2 Holding the rotor yoke with both hands carefully lower it onto the drive shaft Make sure the pin on the drive shaft fits into the groove in the bottom of the rotor yoke ts CAUTION Never drop the yoke onto the shaft The shaft can be bent or broken if the yoke is dropped onto it 13 GH 3 6 Rotor 14 Table 4 Labware for Use with Micro Plus Carriers Required Accessory Part Description Volume Number Description Part Number Rack Multiwell plate 300 uL well 609844 96 well nonsterile pkg 100 Cap strip nonsterile 267002 pkg 10 Deep well polystyrene 267001 EE plate 96 well nonsterile 1 mL well pkg 24 Cap strip sterile 267005 pkg 10 Aluminum foil lid 538619 pkg 100 Cap strip nonsterile 267002 pkg 10 Deep well polystyrene 267004 Td plate 96 well sterile 1 mL well pkg 24 Cap strip sterile 267005 pkg 10 Aluminum foil lidt 538619 pkg 100 Cap strip nonsterile 267002 pkg 10 Deep well 267006 polypropylene plate 1 mL well Cap strip sterile 267005 pkg 10
22. ED LOADING for weight difference information GH 3 6 Rotor Table 2 Modular Disk Adapters Bottle Adapters and Cups Available for the GH 3 8 Rotor continued BLOOD BAG CUPS polypropylene Number Tmax RCF at Bags at Cup Maximum Part Color Cup Size per Bottom Speed Number Code Capacity mm Cup mm x g qty one yellow single bag double pack 90 2 196 2 3090 356856 orange triple pack quad pack 97 1 196 7 3100 356857 AEROSOLVE CANNISTER RACKS polypropylene Adapter Part Nom Nom Max No Max No max at RCF at Number Tube Tube Tubes Tubes Adapter Maximum Color Vol Dia per in Base Speed Set of Set of Code mL mm Adapter Rotor mm x g Two Four white 1 5 11 24 96 174 2740 354495 each blue 3 amp 5 12 24 96 174 2740 359482 359160 tan 5 12 24 96 180 2840 359489 358993 orange 10 14 18 72 175 2760 359483 359161 purple 12 16 12 48 177 2790 359484 359162 3 amp 5 12 6 24 178 2800 white vials 15 14 10 40 174 2740 344517 each green 15 amp 20 18 12 48 174 2740 359485 359163 3 amp 5 12 6 24 176 2770 It green 15 conical 17 6 24 181 2850 359487 358991 3 amp 5 12 6 24 180 2840 round bottom lime green 50 conical 30 4 16 181 2850 359488 358992 3 amp 5 12 4 16 180 2840 round bottom yellow 50 29 4 16 177 2970 359486 359164 3 amp 5 12 4 16 178 2800 orange 230 62 1 4 180 2852
23. Examples of Symmetrically Loaded Trays Examples of Nonsymmetrically load opposite trays the same way Loaded Trays OOo0000000000 OOOOOOOOOOOO O00000000000 OOO0000000000 Ooooooooooooo OOOOOOOOOOOO 000000000000 OooooooooooooO OOOO0O0O0000000 OO00Q00000000 OOOOOOOOOOOO OO0O0000000000 OOOO0O00000000 Figure 4 Examples of Correctly and Incorrectly Loaded Buckets and Carriers Contents of opposing buckets must be the same and each bucket must be balanced on its pivotal axis 18 GH 3 6 Rotor N CAUTION If only two buckets are loaded with blood bags bottles or modular disk adapters the other two buckets should contain at least a minimal blank load such as empty modular disk adapters to achieve optimal results and to avoid rotor imbalance If only two blood bags are being run and modular disk adapters are not available we recommend using two water filled blood bags in cups in the empty buckets Placing empty blood bag cups or bottle adapters in the open buckets does not provide enough weight to prevent possible imbalance Beckman Coulter supplies buckets in weight matched sets to make balancing easier the weight and warranty expiration date are marked on the side of each bucket Modular disk adapters are also sold in weight matched sets However there are variances in weight between sets as well as variance in weight between previously purchased adapters To prevent accidental imbalance it is important to keep
24. GS6 TB 003M y March 2007 INSTRUCTIONS FOR USING THE GH 3 8 SWINGING BUCKET ROTOR In Beckman Coulter Allegra 6 Series GS 6 Series Spinchron Series and GP Series Centrifuges This rotor has been manufactured in a registered ISO 9001 or 9002 facility for use with the specified Beckman Coulter centrifuges 2007 Beckman Coulter Inc Printed in the U S A SAFETY NOTICE This safety notice summarizes information basic to the safe use of the equipment described in this publication The international symbol displayed above is a reminder to the user that all safety instructions should be read and under stood before operation or maintenance of this equipment is attempted When you see the symbol on other pages of this publication pay special attention to the specific safety information presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor system This rotor is warranted for 7 years see the warranty at the back of this publication Beckman Coulter recom mends that you retire the rotor from use at the end of the 7 year warranty period to prevent the possibility of a rotor mishap resulting from material fatigue Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria an
25. IC AND BALANCED LOADING above S CAUTION Buckets are designed to be used only with modular disk adapters bottle adapters and blood bag cups Do not pour samples directly into buckets bottle sleeves or blood bag cups Do not load bottles or blood bags directly into the buckets Do not use labware that is not specified for use in this rotor Assembling Modular Disk Adapters Refer to Table 2 to determine which color adapter is required for the tubes you are using and the number of disks needed to support the length of the tubes l Select the appropriate adapter base from Table 2 and attach a bail to it see Figure 7 Place the base and bail in an empty bucket on the lab bench not in the rotor Position one of the disks in the bucket so that its grooves are aligned with the bail Push the disk down until the bail snaps into the grooves Figure 7 Assembling a Modular Disk Adapter Add more disks in this way until the height of the assembly is nearly as tall as the tubes you will be using If the height of the disks is very tall you may have to push the bail into the grooves of the top disks by hand Remove or add disks to the bail to accom modate shorter or longer tubes If the tubes fit too snugly in the adapter s rubber base apply a light film of powder such as talcum powder to prevent the tubes from sticking GH 3 6 Rotor Loading Tubes in Adapters When placing tubes in modular disk adapters it i
26. NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 905 819 1234 Fax 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 994 07 07 Fax 41 22 994 07 00 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 01 49 90 90 00 Fax 01 49 90 90 10 e mail bioresearch france beckman com GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 513335 Fax 49 21 51333633 e mail bioresearch de beckman com Qi ooo COULTER Beckman Coulter Inc Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 2814 0481 Fax 852 2873 4511 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 1
27. ags bottles except 230 mL conical and tubes up to 130 mm long They are not designed to contain aerosols 25 GH 3 6 Rotor 26 gt NOTE Because of the shape of the covers 130 mm tubes can be used only in the center cavities of the adapters when covers are used Install the cover as follows 1 Make sure that the bucket and the bucket cover surface are clean dry and undamaged 2 Make sure that the O ring 961648 is in good condition and lightly coated with silicone vacuum grease 335148 3 Place the O ring on the ledge inside the bucket see Figure 10 4 Place the cover on the bucket with the latches perpendicular to the pin sockets see Figure 11 5 Snap the latches down and secure them under the bucket rim Insert O ring here Figure 10 Proper Placement of O Ring Snap Down Pin Socket Cover E Latch ep Bucket T NE LL Spring Figure 11 Securing Bucket Cover Latches GH 3 6 Rotor Using Aerosolve Cannisters N CAUTION Do not run chloroformed samples in Aerosolve cannisters Chloroform vapors can damage the cannister material Aerosolve cannisters can be used as wide mouth bottles or with tubes in racks that are specially designed to fit in the cannisters Table 2 lists the Aerosolve tube racks and the number and sizes of tubes they accommodate When Aerosolve cannisters are used in the GH 3 8 rotor buckets pads 361269 must be p
28. amage to the instrument A loose metal object could substantially damage the rotor chamber and lid and could potentially escape the chamber into the labo ratory causing personal injury or property damage The rotor must be run with a full complement of buckets and or multiwell plate carriers attached to the yoke You can run four buckets four multiwell plate carriers or two buckets and two carriers with like components loaded opposite each other If only two buckets are loaded with blood bags bottles or modular disk adapters the other two buckets should contain at least a minimal blank load e g empty modular disk adapters to achieve optimal results and to avoid rotor imbalance The maximum allowable run speed 3750 rpm listed in the rotor specifications is for operation when all condi tions are within the standard specifications using buckets Maximum allowable run speed when using Micro Plus multiwell plate carriers is 3250 rpm Do not overload the rotor without reducing the speed see SPEED DERATING Failure to derate will reduce the safe useful life of the rotor If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appro priate decontamination procedures should be applied to the centrifuge and accessories GH 3 8 ROTOR lmin Axis of Rotation te SPECIFICATIONS Maximum speed buckets llle 3750 rpm Maxi
29. and in place see Figure 3 4 Screw the tie down nut 366642 onto the centrifuge drive shaft N WARNING Do not substitute a metal fastener for the plastic tie down nut 366642 supplied with this rotor If a tie down nut comes off the shaft during centrif ugation the plastic nut furnished will break apart in the chamber causing minimal damage to the instrument A loose metal object however could substantially damage the rotor chamber and lid and could potentially escape the chamber into the laboratory causing personal injury or property damage 5 Insert the torquing bar 356036 through the cross drilled hole in the tie down nut and turn the nut to the right clockwise until it 1s tight Do not overtighten the tie down nut 15 GH 3 6 Rotor gt NOTE If the rotor is left in the centrifuge between runs make sure the rotor is seated on the drive shaft and the tie down nut is tight before each run O Ring Groove Figure 3 Rotor Tie Down Nut and O Ring BUCKETS OR CARRIERS 1 Place buckets or carriers over the pivot pins on the rotor yoke Fill all four positions on the yoke with buckets and or carriers N CAUTION All four positions on the rotor yoke must contain either a bucket or a carrier during a run Never run the rotor with only two positions filled 2 Make sure buckets or carriers are properly seated by gently swinging them on the pivot pins You can use four buckets four carriers or two bu
30. ce by attached latches Although the covers are not designed to contain aerosols that may result from tube breakage they will contain most liquids and broken tube particles reducing the need to clean the centrifuge chamber and allowing you to take appropriate precautions before opening the covers in the event of tube breakage Aerosolve Cannisters Aerosolve cannisters designed to minimize aerosol leakage and liquid spills can be used in the GH 3 8 buckets when this type of containment is required The cannister was tested to demonstrate containment of microbiological aerosols under normal operating condi tions of the associated Beckman Coulter centrifuge when used and maintained as instructed Aerosolve cannisters hold a variety of tube sizes in racks or they can be used as 500 mL wide mouth bottles Validation of microbiological containment was done at an independent third party testing facility CAMR Porton Down UK or USAMRIID Ft Detrick MD U S A Improper use or maintenance may affect seal integrity and thus containment Table 2 Modular Disk Adapters Bottle Adapters and Cups Available for the GH 3 8 Rotor GH 3 6 Rotor MODULAR DISK ADAPTERS polypropylene Nom Nom Max No Max No Imax at RCF at No Adapter Part No Tube Tube Tubes Tubes Adapter Maximum Disks Color Vol Dia per in Bottom Speed per Set of Set of Code mL mm
31. ckets and two carriers with like components positioned opposite each other If two buckets and two carriers are used reduce run speed to the speed allowed for multiwell plate carriers refer to SPEED DERATING below 16 GH 3 6 Rotor LOADING THE ROTOR WORKING WITH PHYSIOLOGICAL FLUIDS N WARNING Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Take appropriate safety precautions when handling toxic pathogenic or other hazardous materials When working with potentially hazardous materials always fill or open containers in an appropriate hood or biological safety cabinet Three levels of containment are offered by Beckman Coulter and can be used singly or combined depending upon your application 1 Capped tubes or bottles are designed to provide fluid containment We strongly recom mend that all containers carrying physiological fluids be capped to prevent leakage 2 Bucket covers for the GH 3 8 rotor help to contain fluids within the bucket in the event of tube breakage or blood bag failure 3 Aerosolve cannisters are designed to minimize the possibility of aerosol and fluid leakage during centrifugation SYMMETRIC AND BALANCED LOADING To ensure
32. d certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good lab oratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organiza tion Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection Dispose of all waste solutions according to appropriate environmental health and safety guidelines The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials such as chloroform or ethyl alcohol in or handle or store them near the centrifuge If glass tubes break remove the glass very carefully from the adapter bucket or cavity If all the glass particles are not contained in the bucket or cavity be careful when examining or cleaning the centrifuge gasket and chamber as glass particles may be embedded in their surfaces Inspect the rotor once a month especially inside cavities for rough spots or pitting white powder deposits frequently aluminum oxide or heavy dis coloration If any of these signs are evident
33. eckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center Regularly check the condition of the O ring 927571 in the tie down nut if it is worn or damaged replace it Regularly check the condition of the Micro Plus plate carrier base 361302 and pad 362390 and do not use them if there are visible signs of damage MAINTENANCE Buckets and Micro Plus carriers are made of anodized aluminum Do not use sharp tools on them as scratches in the anodized surface may lead to corrosion Approximately once a week and after cleaning and or autoclaving wipe the rotor pins and pin sockets see Figure 13 with a paper towel then coat with Paint On Graphite Lubricant 977212 Allow the lubricant to dry for at least 5 minutes before installing the rotor in a centrifuge Before using the tie down nut 366642 check it for damage such as distortion splitting or stripped threads replace it if it is damaged Inspect the O ring 927571 in the base of the nut for cracks replace if damaged Do not use any type of lubricant or grease on the threads of the tie down nut Before first use of the rotor and after washing apply Anti Seize 961660 to the rotor yoke drive hole as described under CLEANING below Anti Seize is a registered trademark of Permatex 33 GH 3 6 Rotor 34 Lubrication Points all pins Lubrication Area both pin sockets
34. g plastic gloves open the cannister and immerse the cannister lid and contents intact in a 2 liter beaker or similar vessel containing 5 sodium hypochlorite undiluted bleach 2 Leave the cannister to soak overnight 3 Carefully dislodge the tube rack and scrub it with a brush 4 Decant the bleach into a waste bottle for proper disposal 5 Rinse with water and air dry the cannister and tube rack 6 Flammability hazard Do not use in or near operating centrifuges GH 3 6 Rotor RETURNING A ROTOR Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form which may be obtained from your local Beckman Coulter sales office should contain the following information serial number history of use approximate frequency of use reason for the return original purchase order number billing number and shipping number if possible name and phone number of the person to be notified upon receipt of the rotor or acces sory at the factory and name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are free from pathogens and or radioactivity Sterilization and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag
35. ge align the blood bag seam with the rotor pivot pins see Figure 9 with the label facing out 5 Place bucket covers on rotor buckets to prevent blood bags or tubing from contacting the rotor yoke during centrifugation GH 3 6 Rotor Blood Bag Label Sandwich Coiled Blood Bag Seam Tubing between Ports Aligned with Transfer Bags Pivotal Axis of and Filled Bucket Blood Bag Pss Blood Bag Seam Blood Bag Label dp E Blood Bag i Label Blood Bag Label Blood B Blood Bag Label ood Bag eup Figure 9 Blood Bag Loading Procedures N CAUTION If bucket covers are not used make sure the super structure of the blood bag protruding from the cup does not inhibit the bucket from reaching its horizontal position If it does remove the cup from the rotor and reposition the blood bag so that it seats further into the cup Allowing the blood bags to contact the rotor yoke during centrifugation can cause the bucket to come off the pivot pins and can seriously damage both the rotor and the centrifuge If only two filled cups are run place them in opposing buckets The other two buckets should contain similar blank loads to prevent imbalance either empty modular disk adapters or water filled blood bags in cups Bucket Covers Beckman Coulter recommends that you use bucket covers 360586 to help contain spills and glass particles in the event of tube breakage Bucket covers can be used with all blood b
36. ividually These tubes cannot be run with bucket covers in place 4 Do not load the 750 mL bottle directly into the bucket always use the adapter Replacement bottle cap part number is 356263 set of 6 GH 3 6 Rotor Table 3 Beckman Coulter Tubes and Bottles for the GH 3 8 Rotor continued Adapter Volume Part Dimensions mL Description Number Set of Two Set of Four OPEN TOP TUBES continued 29 x 104 mm 50 polycarbonate 363075 359474 359153 graduated pkg 8 3594862 3591642 29 x 103 mm 50 polypropylene 357007 359474 359153 3594862 3591644 29 x 103 mm 50 polycarbonate 363647 359474 359153 3594862 3591642 TUBES WITH SNAP ON CAPS 11 x 38 mm 1 5 polyallomer 357448 359469 359148 35451 16 354495 11 x 38 mm 1 5 polypropylene 356090 359469 359148 354511 3544952 11x 38 mm 1 5 blue 356091 359469 359148 polypropylene 354511 354495 11x 38 mm 1 5 green 356092 359469 359148 polypropylene 354511 3544952 11x 38 mm 1 5 yellow 356093 359469 359148 polypropylene 35451 16 3544952 11x 38 mm 1 5 orange 356094 359469 359148 polypropylene 35451 16 3544952 11 x 38 mm 1 5 polypropylene 343169 359469 359148 cap separate 35451 16 354495 11x 39 mm 1 8 white 340196 359469 359148 polyethylene 354511 3544958 29 x 103 mm 50 polypropylene 357005 359474 359153 3594863 3591642 29 x 103 mm 50 polycarbonate 363664 359474 359153 3594863 3591642 Adapter used in Aerosolve ca
37. l appropriate safety precautions are taken Also see WORKING WITH PHYSIOLOGICAL FLUIDS above N CAUTION Make sure the rotor is seated on the drive shaft and the tie down nut is tight before each run Refer to the appropriate instrument instruction manual for centrifuge operation It is best to precool or warm the rotor to the desired temperature before installing it in the centri fuge Tables 6 and 7 indicate approximate acceleration and deceleration times for this rotor in both 60 Hz and 50 Hz instruments Table 5 Recommended g Force Limits to Minimize Breakage of Certain Glass Tubes and Bottles Made by Other Manufacturers in the GH 3 8 Rotor Safe g Safe Modular Volume Dimensions Force Speed Disk Adapter Tube Description mL mm RCF RPM Color Pyrex Kimax B D or Dispo 3 10x 75 max 3750 blue RB or FB or less Pyrex LT 3 11x75 max 3750 blue B D Vac RB plain 5t 12x 75 max 3750 blue Kimax RB 5 12x 75 max 3750 blue Pyrex 9820 RB 5 12x 75 max 3750 blue B D SST RB SB 7 13 x 100 max 3750 orange B D Vac RB plain 7 13 x 100 max 3750 orange B D SST RB SB 10 16 x 100 3000 3600 purple B D SST RB plain 10 16 x 100 max 3750 purple Kimax 45049 RB 10 16 x 100 max 3750 purple Kimax 45200 LT G SC 10 17 x 116 max 3750 purple Pyrex 9820 RB 10 16 x 100 max 3750 purple Pyrex 8140 LT G 12 17 x 117 max 3750 purple B D SST RB SB 15 16 x 130
38. laced beneath the cannisters in the buckets to provide support and prevent the cannisters from being damaged during centrifugation These pads are included in the GH 3 8 Aerosolve cannister kits 359232 N CAUTION Be sure to remove the pad when you remove the cannister placing other accessories on top of the pad could cause tube breakage rotor imbalance improper run results or rotor mishap Tubes bottles or blood bags placed in labware that rests on Aero solve cannister pads will be closer to the rotor yoke and can interfere with the proper swing of the bucket If using tube racks load them symmetrically refer to Figure 4 If only two cannisters are being run place the cannisters in opposite buckets place empty adapters or blank loads in the two empty buckets for balance Read publication TJ6 TB 011 supplied with the cannister for complete information regarding its use and maintenance USING Micro Plus MULTIWELL PLATE CARRIERS Micro Plus plate carriers see Figure 12 can be used in place of the buckets normally used with the rotor For complete information about the carriers see publication GS6 TB 011 which accompanies the carriers If only two carriers are run they must be installed opposite each other in the rotor and either two buckets or two other carriers must also be run they need not be loaded to prevent rotor imbalance The rotor should never be run with only two buckets or two carriers installed Oppo
39. mum speed Micro Plus carriers 3250 rpm Critical speed range 00 000 400 to 1450 rpm Maximum solution density slele esee 1 2 g mL Relative Centrifugal Fields at maximum speed see Table 1 for RCF at other speeds using buckets Fmax 204 mm 2 20 ee eee 3210 x g using Micro Plus carriers fmax 163 mm 1924 x g Conditions requiring speed reduction see SPEED DERATING Number buckets or carriers 0 0 eee ee eee 4 Available tubes and bottles 0 0 02 lees see Table 3 Maximum load allowed in each bucket at rated speed excluding weight of bucket and cover 1000 grams Maximum load allowed in each Micro Plus carrier at rated speed excluding weight of carrier 500 grams Maximum rotor capacity lees 3 liters Approximate acceleration and deceleration times 0 000 00s see Tables 6 and 7 Weight of fully loaded rotor buckets with covers 0000 eee eeee 11 4 kg 25 2 Ib Rotor yoke material llle stainless steel Bucket material 0 0 0 eee eee eee anodized aluminum Carrier material 0 0 eee eee eee eee anodized aluminum The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration Relative Centrifugal Field RCF is the ratio of the ce
40. nee see Table 2 Neoprene tube cushions for blue rack pkg of 24 2 0 eee eee 344117 for orange rack pkg of 18 2 2 ee ee eee 344118 for purple rack pkg of 18 12 large 6 small 0 0004 344119 for dark green rack pkg of 18 12 large 6 small 344120 for yellow rack pkg of 8 4 large 4 small 00 0000 344121 Blood bag cup 90 mm yellow polypropylene 0004 356856 40 Blood bag cup 97 mm orange polypropylene 0000 356857 Balancing pads six pads 3 grams each red 000 358365 Balancing pads six pads 6 grams each grey 00000 358364 Microfuge tube rack inserts Blue insert 13 mm dia 25 pkg 0 0 oe ee 373697 Red insert 12 mm dia Q5 pkg 2 0 ee eee 373698 White insert 11 mm dia for 1 5 mL Microfuge tubes 25 pkg 373696 Black insert 10 mm dia 25 pkg 0 eee eee eee eee 373699 Rotor Cleaning Kit cecar sted eee eked Gob Ree ette Rer 339558 Solution 555 bgt oaa steep RUE Rr Rp es 339555 Silicone vacuum grease 202 seeeeeeeeee hn n 335148 Anti Seize 1 1 2002 i scien Siesta CORE Sa ww epe E e RU 961660 Paint On Graphite Lubricant 00 0 eee cee ee 977212 GH 3 6 Rotor 41 BENCHTOP ROTOR WARRANTY Subject to the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and condi
41. nnister part number 359232 Continued b Tube retainers and adapter plates are sold individually These tubes cannot be run with bucket covers in place 4 Do not load the 750 mL bottle directly into the bucket always use the adapter Replacement bottle cap part number is 356263 set of 6 10 GH 3 6 Rotor Table 3 Beckman Coulter Tubes and Bottles for the GH 3 8 Rotor continued Adapter Volume Part Dimensions mL Description Number Set of Two Set of Four CONICAL TUBES 17 x 120 mm 15 polypropylene 355663 359472 359151 graduated 3594872 35899 12 62 x 141 mm 230 polycarbonate 356987 3499465 356983 with cap 3569852 62 x 141 mm 230 polypropylene 356989c 3499465 356983 with cap 3569854 BIOVIALS 14 x 55 mm 4 polypropylene 566353 359470 359149 3445174 each BOTTLES 29 x 104 mm 50 polycarbonate 361693 359474 359153 with cap assembly 3594862 3591642 29 x 104 mm 50 polycarbonate 357002 359474 359153 with screw cap 3594862 3591642 29 x 104 mm 50 polyallomer 357001 359474 359153 with cap assembly or 361694 3594862 3591642 29 x104 mm 50 polyallomer 357003 359474 359153 with screw cap 3594862 3591642 28 5 x 107 mm 50 Teflon with high 363076 359474 359153 speed screw cap 3594862 359164a 62 x 141 mm 230 conical wide mouth 356987 356983 polycarbonate use w 349946 62 x 141 mm 230 conical wide mouth 356989c 35
42. nnot be run with bucket covers in place 4 Do not load the 750 mL bottle directly into the bucket always use the adapter Replacement bottle cap part number is 356263 set of 6 N CAUTION Do not run chloroformed samples in Aerosolve cannisters Chloroform vapors can damage the cannister material 12 GH 3 6 Rotor Micro Plus MULTIWELL PLATE CARRIERS Micro Plus multiwell plate carriers see Figure 1 are used by installing them on the pivot pins in place of the buckets normally used with the rotor Micro Plus carriers are made of aluminum and are black anodized for corro sion resistance The design of this carrier allows higher operating speeds than were possible in older carrier designs A blue thermoplastic base is used to house the multiwell plates A flexible plastic pad between the base and the plate s supports the plates during a run Each Micro Plus carrier will accommodate a deep well multi well plate or can run up to three 96 well microplates when more than one are run one plate is placed directly on top of the other Refer to Table 4 for a list of labware that can be used with the carrier Multiwell plates will break if g forces are too high Rotor speed must not exceed 3250 rpm when Micro Plus carriers are run and additional speed reduction is required if maximum load limits 500 grams per carrier are exceeded Figure 1 The Micro Plus Multiwell Plate Carrier INSTALLATION THE ROTOR YOKE gt NOTE
43. ntrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 9 amp where r is the radius in millimeters is the angular velocity in radians per second 2 tRPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution 2 RCF 1 127 REM 1000 GH 3 6 Rotor Table 1 Relative Centrifugal Fields for the GH 3 8 Rotor Entries in the table are calculated from the formula RCF 1 12 r RPM 1000 and are then rounded to three significant digits Relative Centrifugal Field x g at fmax Rotor Speed Buckets Micro Plus Carriers rpm 204 mm 163 mm 3750 3210 3500 2800 Don t run above 3250 3250 2400 1928 3000 2060 1643 2750 1730 1381 2500 1430 1141 2250 1160 924 2000 913 730 1750 700 559 1500 514 411 1000 228 183 500 57 46 Relative Centrifugal Field RCF GH 3 8 Rotor 3500 3000 2000 amp 2500 P Buckets 5 1909 Micro Plus 1000 500 0g 1000 2000 3000 4000 Speed rpm GH 3 6 Rotor DESCRIPTION Specific information about the GH 3 8 rotor is given here Information about the use and care of centrifuges is contained in the individual instrument instruction manuals which should be used in combination with this manual for complete centrifuge and rotor opera tion information THE ROTOR The GH 3 8 rotor rated fo
44. o run a tube of the same approximate weight in the same configuration in the opposite bucket In multiwell plate carriers samples should be loaded into the wells symmetrically with respect to the pivotal axis of the carrier the pivotal axis runs parallel to the crossbar and opposing carriers should contain the same loads see Figure 4 N CAUTION gt NOTE If multiwell plate carriers are not loaded symmetri cally with respect to each carrier s pivotal axis the carriers could come off the rotor pivot pins and could damage the rotor and the centrifuge The same adapters can be used in the GH 3 8 and GH 3 7 rotors see Table 2 However do not intermix adapters or their individual parts from Beckman Coulter J6 series rotors with those for the GH 3 8 rotor While the adapters are very similar in appearance see Figure 5 they have very different weights J6 adapters have bails that are curved at the top bails for the GH 3 8 adapters are straight Keep J6 and GH 3 8 disks and bases separate mixing them can cause imbalance Figure 5 The Bails Vertical Supports of the GH 3 8 and GH 3 7 Modular Disk Adapters and the J6 Series Adapters are Not the Same Bails for the GH 3 8 are straight and end in a disk bails for the J6 have curved ends Do not intermix adapters or disks from adapters between sets During a run buckets and carriers swing 90 degrees from their at rest position The pivotal axis of a bucket or carrier
45. optimal performance and stability the GH 3 8 rotor must be loaded symmetri cally see Figure 4 Two factors affect symmetric loading The buckets must be loaded symmetrically with respect to their pivotal axes The rotor should be loaded symmetrically with respect to its center of rotation This means that for best results you should load opposing buckets or carriers with the same type of labware containing the same amounts of fluid of equal density Additionally opposing buckets and their contents should weigh approximately the same within 6 grams Do not exceed the rated maximum load for either buckets 1000 grams each including labware and sample or Micro Plus carriers 500 grams each including base pad plate s and sample 17 GH 3 6 Rotor BALANCED LOAD UNBALANCED LOAD Pivotal Axis of Bucket Center of Rotation CORRECT INCORRECT eeooooee 00000000 ooe OOOOOOOO O OOOOOOOO eeooooee 000008000 ooe OoOoOOOOOO O OOOOOOOO eeooooee oooooooo 000 OOOOOOOO O OOOOOOOO eeooooee oooooooo 000 OOOOOOOO O OOOOOOOO eeooooee oooooooo 000 00000000 O OOOOO0OO eooooee OOOOOOOO ooe 00000000 O OOOOOOOO eeooooee oooooooo 000 00000000 O eeeeeoee eeooooee oooooooo 000 00000000 O 0e L4 4 eeooooee oooooooo 000 OOOOOOOO Oj e eeooooee oooooooO 000 OoOOoOOOOO Oj e eeooooee 00000000 ooe OOOOOOOO O e e eecoooee eeeeeeee ooe OOOOOOOO Oj je e
46. r 3750 rpm is used in Beckman Coulter Allegra 6 series GS 6 series Spinchron series and GP series centrifuges This four place swinging bucket rotor carries a wide range of tube and bottle sizes from 1 5 to 750 mL or single to quad pack blood bags in buckets as well as 96 well multiwell plates in specially designed carriers This rotor rapidly sediments protein precipitates large particles cells and cell debris It can also be used for binding studies and for separating serum from whole blood The rotor yoke is made of stainless steel Either buckets or multiwell plate carriers both made of anodized aluminum may be run by placing them over pivot pins on the arms of the yoke Both buckets and carriers swing out to a horizontal position during centrifugation N CAUTION Although rotor components and accessories made by other manufacturers may fit in the GH 3 8 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the GH 3 8 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor BUCKETS AND ACCESSORIES Several types of labware can be placed in the buckets depending upon your application modular disk adapters for tubes of various sizes bottle adapters blood bag cups and Aerosolve cannisters when aerosol containment is
47. required Bucket covers are also available as an aid toward containment GH 3 6 Rotor Modular Disk Adapters Tubes are supported in modular disk adapters which can also serve as tube racks in the laboratory The adapter disks are color coded by the tube size they accommodate see Table 2 the number of disks used in an adapter assembly depends upon the length of tubes used A tube decanter is available to hold either 10 mm or 12 mm tubes securely in the blue adapter allowing all the tubes to be decanted at once Additionally 1 5 mL Microfuge tubes can be run using a special plate that fits on top of the blue adapter Both of these accessories are described in Table 2 Beckman Coulter tubes and bottles available for use in the GH 3 8 rotor buckets are described in Table 3 Bottle Adapters Bottles are supported in polypropylene adapters that fit inside the rotor buckets The adapters are ribbed for strength and accommodate three bottle sizes including one conical bottle see Table 2 Blood Bag Cups Polypropylene cups provide support for blood bags in the rotor buckets Blood bag cups are available in two sizes one for single or double pack bags and one for triple or quad pack bags see Table 2 Bucket Covers Transparent covers made of a high impact plastic are available for the GH 3 8 rotor buckets Each cover requires an O ring made of ethylene propylene that seats on a ledge inside the bucket The covers are held in pla
48. revent rotor imbalance If modular disk adapters are not available use two water filled bottles in adapters to balance the load See SYMMETRIC AND BALANCED LOADING above Using Blood Bag Cups gt NOTE This rotor is suitable for obtaining cell free plasma or for cell packing not for separating blood components The yellow 90 mm cup 356856 accommodates up to two single bags or one double pack the orange 97 mm cup 356857 supports either one triple or one quad pack Do not pour liquid directly into blood bag cups Fit blood bags into cups before loading the cups into the rotor buckets you can trip the imbalance detector in the centrifuge by pushing blood bags into cups within buckets installed in the rotor Load the blood bag cups as follows 1 Load the bags as far down into the cups as possible gt NOTE Make sure the bags stay as vertical as possible with no folds at the top or corners If folds are present blood cells could remain in the folds and then mix with the plasma when the bag is removed 2 Sandwich the tubing between the blood bag and any transfer packs see Figure 9 3 Make sure the loaded blood bag cups opposite each other on the rotor yoke are approx imately the same weight within 1 gram Soft pliable balancing pads that will not puncture blood bags see the SUPPLY LIST can be used if necessary to maintain weight balance 4 Place loaded cups into rotor buckets To reduce the possibility of bag breaka
49. s important to make sure that they will not contact the rotor yoke during a run One way to do this is to place empty tubes in an adapter place the adapter in a bucket on the rotor yoke and manually swing the bucket to the horizontal position making sure that all tubes clear the yoke Another method is to place the adapter in a bucket and position a bucket cover over the tubes checking that tubes do not touch the cover If all positions in an adapter are not filled the adapter should be loaded symmetrically with respect to its pivotal axis see SYMMETRIC AND BALANCED LOADING above Loading Adapters in the Buckets After placing tubes in the disk adapters lower the adapters into the buckets so that the bails line up with the rotor pivot pins see Figure 8 If only two loaded adapters are run place them in opposing buckets The other two buckets should contain empty adapters to prevent imbalance See SYMMETRIC AND BALANCED LOADING above n Pivot Pin Figure 8 Installing an Adapter in a Bucket Be sure the bails vertical supports line up with the bucket pivot pins mil 23 GH 3 6 Rotor 24 Using Bottles in Adapters Load bottles into their appropriate adapters see Table 2 then place the filled adapters in the rotor buckets If only two bottles are run place them in opposite buckets Make sure the other two buckets contain at least a minimal blank load such as empty modular disk adapters to p
50. sing Micro Plus carriers must balance to within 10 grams See SYMMETRIC AND BALANCED LOADING above 27 GH 3 6 Rotor Deep well Plate set Carrier Figure 12 The Micro Plus Multiwell Plate Carrier Base Pad and Deep well Plate gt NOTE If symmetrical pelleting is required use round U bottom or V bottom multiwell plates commercially available from many suppliers for best results Load a multiwell plate into a carrier as follows 1 To prevent plate breakage during centrifugation place the flexible plastic pad 362390 ridged side up into the flat indented area of the blue base 361302 2 Place the filled labware on top of the pad being careful not to spill the contents If you are using two or three microplates place a 96 well cap strip 267002 between the plates to prevent plate breakage during centrifugation gt NOTE If running stacked polystyrene plates place a 96 well cap strip 267002 beneath the bottom plate as well as between each set of plates 3 Slide the base pad and plate assembly into the carrier until the base locating feet click into place 28 GH 3 6 Rotor IF YOU USE MORE THAN ONE GH 3 8 ROTOR Complete sets of buckets Micro Plus plate carriers and labware may be used in any GH 3 8 rotor Remember the following points e Buckets are sold in weight matched sets Keep matched sets together and check their weights prior to use to avoid centrifuge imbalance
51. this warranty for seven 7 years from the date of purchase and will be replaced or repaired during such period according to the terms and conditions of this warranty The date of manu facture marked on the bucket may be earlier than the date of purchase and the expiration date marked on the bucket which is seven 7 years after the date of pur chase may be correspondingly offset 5 Buckets should not be used after the expiration date marked on the bucket If at the time of purchase the marked expiration date is less than 7 years from the date of purchase the expiration date becomes the date of pur chase plus seven 7 years use of a bucket after such expiration date voids Beckman Coulter s warranty obli gations with respect to any rotor and or centrifuge in which such a bucket is used 6 Thermoplastic rotors or components used in some bench top centrifuges are warranted for one 1 year from date of purchase DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BEIN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR RE PLACEMENT OF THE PRODUCT Beckman Coulter Worldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville
52. tions in effect at the time of sale Beckman Coulter agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of a benchtop centrifuge rotor to the orig inal buyer by Beckman Coulter or by an authorized represen tative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge all centrifuge parts required for repair CONDITIONS Except as otherwise specifically provided herein this warranty covers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor characteristics or operated in a Beckman Coulter centri fuge that has been improperly disassembled repaired or modified 4 Each bucket whether purchased with a rotor assembly or purchased separately is covered by
53. uare root reduction formula to determine the allowable rotor speed 500 grams d 3250 M 2 Speed ey d heaviest load in grams 2 29 GH 3 6 Rotor 30 Reduce rotor speed if the desired rotor temperature when run in a refrigerated centri fuge is lower than 3 C and ambient temperature is higher than 25 C e When certain plastic tubes made by other manufacturers are used in Aerosolve cannister tube racks you must reduce speed to prevent tube deformation or breakage as these racks do not provide full tube support Beckman Coulter recommends that you pretest other manufacturers tubes in these racks using water samples to determine run speed and duration reductions to avoid tube deformation or breakage e When using certain glass tubes you must reduce rotor speed to prevent tube breakage Table 5 indicates recommended g force limits to minimize breakage and is intended as a guide only Because the strength of glass and plastic tubes can vary between lots and will depend on handling and usage Beckman Coulter highly recommends that you pretest these tubes in the appropriate labware in the GH 3 8 rotor using water samples to determine optimal operating conditions gt NOTE Scratches even microscopic ones significantly weaken glass tubes RUN PROCEDURE N WARNING Operator error or tube failure may generate aerosols Toxic pathogenic or other hazardous materials must not be run in this rotor unless al
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